Cell and tissue kinetics最新文献_第6页

Factors modifying the synergistic toxicity of deoxycytidine in combination with thymidine plus 5-fluorouracil in HeLa cells. 修饰脱氧胞苷与胸苷加5-氟尿嘧啶联合在HeLa细胞中的协同毒性的因素。

Cell and tissue kinetics Pub Date : 1983-11-01

J Fried, A G Perez, J M Doblin, B D Clarkson

{"title":"Factors modifying the synergistic toxicity of deoxycytidine in combination with thymidine plus 5-fluorouracil in HeLa cells.","authors":"J Fried, A G Perez, J M Doblin, B D Clarkson","doi":"","DOIUrl":"","url":null,"abstract":"We reported previously that deoxycytidine (CdR) enhances the cytotoxic effects of the drug combination thymidine (TdR) plus 5-fluorouracil (FUra) against HeLa S-3 cells. We have now examined the relationships between the concentration of CdR and its cytotoxic and cytokinetic effects, and have also investigated the role of certain other components of the culture medium in this phenomenon. Cell survival was determined by a colony-forming assay; cytokinetic effects were monitored by flow cytometry. In the initial experiments, cells were grown in Ham's F12 medium and exposed for 22 hr to 4 mM TdR, 0 X 025 mM FUra, and dCyd ranging from 1 microM to 4 X 0 mM. The individual drugs were at most only slightly toxic under these conditions; for TdR plus FUra, the survival decreased to 50% (in 5% FCS), and in the three-drug combination it varied from 8% at 1 microM CdR to 28% at 0 X 10 mM and back to a low of 3% at 4 X 0 mM CdR. Results from flow cytometry appeared correlated with the survival data, in that cells accumulated in the S phase to a greater extent in the region around 0 X 10 mM CdR than at higher or lower concentrations. When cells were exposed to the drugs in MEM medium in place of F12, their sensitivity to FUra and the TdR-FUra combination was enhanced, although the additional synergistic effect of CdR was reduced. We found that hypoxanthine, present in F12 but not in MEM, was the principal compound responsible for the observed differences between media.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 6","pages":"539-48"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17683295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reparative growth in the rat thyroid. 大鼠甲状腺的修复性生长。

Cell and tissue kinetics Pub Date : 1983-11-01

B M Stringer, D Wynford-Thomas, H R Harach, E D Williams

引用次数: 0

Generation time of leukaemic blast progenitor cells. 白血病母细胞祖细胞的生成时间。

Cell and tissue kinetics Pub Date : 1983-11-01

M Minden, P Major, J Griffin, A Wu, D Kufe

引用次数: 0

Quantitative study of deoxycytidine incorporation in large and small lymphocytes of the mouse. 小鼠大、小淋巴细胞脱氧胞苷掺入的定量研究。

Cell and tissue kinetics Pub Date : 1983-11-01

K Hamatani, A Kawahara, M Amano

{"title":"Quantitative study of deoxycytidine incorporation in large and small lymphocytes of the mouse.","authors":"K Hamatani, A Kawahara, M Amano","doi":"","DOIUrl":"","url":null,"abstract":"Using radioautographic smear preparations of thymocytes and mesenteric lymph node (MLN) cells labelled with three different tritiated pyrimidine deoxyribonucleosides, the incorporation of DNA precursors was studied separately on large lymphocytes and small lymphocytes. Radioautographic reaction due to generally tritiated deoxycytidine ( [G-3H]CdR) labelling in vivo in large lymphocytes was more intense than that in small lymphocytes. When mice were sacrificed 6 hr after the administration of tritiated thymidine ( [3H]TdR), small lymphocytes were labelled more heavily than large lymphocytes. However, labelling intensity with [3H]TdR in large lymphocytes was greatly enhanced by the administration of 5-fluoro-deoxyuridine, whereas in small lymphocytes labelling intensity was only fairly enhanced by the same treatment. When cells were incubated in vitro with 5-tritium labelled deoxycytidine [( 5-3H]CdR) for 10 min, there was no significant difference in labelling intensities between large and small lymphocytes. In the case of [G-3H]CdR incorporation, the labelling intensity in large lymphocytes was found to be significantly stronger than that in small lymphocytes. Large as well as small lymphocytes incorporated [3H]TdR very well in vitro. However, addition of 5 X 0 X 10(-5) M of non-radioactive CdR to the medium greatly decreased the incorporation of [3H]TdR by large lymphocytes, whereas the effect of non-radioactive CdR in small lymphocytes was not so marked as that in large lymphocytes. Furthermore, the [3H]TdR-labelling percentages were decreased at the same rate by the addition of non-radioactive CdR in both large and small lymphocytes. These results indicate that large lymphocytes and a proportion of small lymphocytes have a strong tendency to convert CdR to thymidine mono-phosphate, which is utilized for DNA synthesis, whereas this ability is relatively weak in the rest of small lymphocytes. Thus, it is probably that this metabolic ability changes during the transition of the large lymphocyte to the small lymphocyte.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 6","pages":"557-70"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17661501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diurnal variation of DNA synthesis in premalignant hamster cheek pouch. 癌前仓鼠颊袋DNA合成的日变化。

Cell and tissue kinetics Pub Date : 1983-11-01

L M Lin, R A Goepp

引用次数: 0

Polyamine-mediated inhibition of in-vitro cell proliferation is not due to acrolein. 多胺介导的体外细胞增殖抑制不是由于丙烯醛。

Cell and tissue kinetics Pub Date : 1983-11-01

J I Hussain, C J Smith, J C Allen

引用次数: 0

Erythroid-committed progenitors and spleen colony-forming cells in adult thymus-deprived mice. 成年胸腺剥夺小鼠的红系祖细胞和脾脏集落形成细胞。

Cell and tissue kinetics Pub Date : 1983-09-01

P Milenković, L Biljanović-Paunović, M Lukic, V Pavlović-Kentera

引用次数: 0

Accumulation of non-cycling cells with a G2-DNA content in ageing solid tumours. Study of the Ca 755 mammary adenocarcinoma of mice. 老化实体肿瘤中含有G2-DNA的非循环细胞的积累。小鼠乳腺ca755腺癌的研究。

Cell and tissue kinetics Pub Date : 1983-09-01

P Coninx, F Liautaud-Roger, A Boisseau, M Loirette, A Cattan

引用次数: 0

The percentage labelled mitoses technique shows the mean cell cycle time to be half its true value in Carcinoma NT. I. [3H]thymidine and vincristine studies. 百分比标记有丝分裂技术显示癌NT的平均细胞周期时间是其真实值的一半。[3H]胸苷嘧啶和长春新碱研究。

Cell and tissue kinetics Pub Date : 1983-09-01

E Hamilton, J Dobbin

{"title":"The percentage labelled mitoses technique shows the mean cell cycle time to be half its true value in Carcinoma NT. I. [3H]thymidine and vincristine studies.","authors":"E Hamilton, J Dobbin","doi":"","DOIUrl":"","url":null,"abstract":"Cell kinetic parameters measured by the percentage labelled mitoses (PLM) technique have been compared with those derived from continuous labelling and stathmokinetic data in the mouse tumour Carcinoma NT. In [3H]TdR labelled tumours, a PLM curve showed the length of DNA synthesis (TS) to be 9 hr. With continuous labelling, 2.5% of cells entered S phase per hour and TS was 16 hr. Six per cent of cells incorporated [3H]TdR between 1 and 3 hr after an injection of the label. The stathmokinetic technique showed that the rate of entry to mitosis was 2.0% hr. The turnover time (TT) of the population was found to be 16 hr with the PLM data and 35 and 37 hr with the continuous labelling and stathmokinetic data, respectively. The PLM technique therefore showed the mean cell cycle parameters to be about half the values obtained with other methods of measurement. We conclude that in Carcinoma NT there is a wide range of cell cycle times. The continuous labelling and stathmokinetic data represent the mean cell cycle parameters, while the PLM data give the minimum values. A cell loss factor calculated from the PLM data would, therefore, be too high by a factor of two.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 5","pages":"473-81"},"PeriodicalIF":0.0,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17933510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell kinetics of early gestation mouse uterus. 早孕小鼠子宫细胞动力学。

Cell and tissue kinetics Pub Date : 1983-09-01

R Herken

引用次数: 0