Cell and tissue kinetics最新文献_第7页

Theoretical implications for cell migration through the crypt and the villus of labelling studies conducted at each position within the crypt. 在隐窝内的每个位置进行的标记研究对细胞通过隐窝和绒毛迁移的理论意义。

Cell and tissue kinetics Pub Date : 1983-09-01

S Tsubouchi

{"title":"Theoretical implications for cell migration through the crypt and the villus of labelling studies conducted at each position within the crypt.","authors":"S Tsubouchi","doi":"","DOIUrl":"","url":null,"abstract":"The migration and turnover of epithelial cells was examined in the crypt and villus of the mouse jejunum using 1-micron plastic and 6-microns paraffin sections both with or without [3H]thymidine radioautography. The mean diameter of columnar cells was constant at 13.8 microns throughout the crypt, whereas it gradually increased from 16.8 microns in the lower villus to 25.4 microns in the upper villus. The cell cycle time (Tc) and the duration of DNA synthesis (ts) obtained by plotting labelled mitoses against time after [3H]thymidine injection, were 13.4 and 7.5 hr respectively. The labelling index (LI) at the various cell positions in the crypt, of mean length 27.5 cells, averaged 28.6% with a peak of above 55% in positions 7 to 11. The migration velocity of cells in the villus epithelium was estimated from the leading labelled cells at 2.04 cell/hr. The fraction of epithelial cells present in crypt and villus was 0.44 and 0.56 respectively. The migration velocity in the crypt (expressed as cell positions entered per hour), calculated from a cumulative cell production rate (LI/ts) in each cell position, increased in the lower and middle crypt (that is, in the proliferative zone, cell positions 1-18), but remains constant at a level of 1.0 in the upper crypt in the non-proliferative zone (cell positions 19-28). Therefore the migration velocity increases from 1.05 cell/hr in the upper crypt to 2.04 at the base of villus. Accordingly the velocity (expressed as distance traversed per hour) also shifts from 13.8 microns in the upper crypt to 34.2 microns in the base of villus and further increases to 51.8 microns in the upper villus because of continuous enlargement of the cells. The transit times of epithelial cells through the non-proliferative upper crypt (above ten cell positions) and the villus (67.9 cell positions on the average) are 8.4 and 33.4 hr respectively. The turnover times of crypt, villus, and whole epithelium are estimated at 26.5, 33.4, and 59.6 hr respectively.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 5","pages":"441-56"},"PeriodicalIF":0.0,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17933508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidermal keratinocytes actively maintain their intracellular polyamine levels. 表皮角质形成细胞积极维持细胞内多胺水平。

Cell and tissue kinetics Pub Date : 1983-09-01

D I Roseeuw, C L Marcelo, L M Rhodes, J J Voorhees

{"title":"Epidermal keratinocytes actively maintain their intracellular polyamine levels.","authors":"D I Roseeuw, C L Marcelo, L M Rhodes, J J Voorhees","doi":"","DOIUrl":"","url":null,"abstract":"Increased cellular polyamine levels are thought to be essential for epidermal keratinocyte proliferation. However, a number of studies report that the induction of keratinocyte proliferation and of ornithine decarboxylase, the rate-limiting enzyme of putrescine, spermidine and spermine biosynthesis, is not concordantly expressed. The relationship between epidermal keratinocyte polyamine synthesis and proliferation was studied in neonatal mouse keratinocyte cultures using specific inhibitors of ODC activity to decrease the intracellular polyamine levels. The ODC inhibitors alpha-methyl ornithine (alpha-Me-Orn), alpha-hydrazino ornithine (alpha-HO) and difluoro-alpha-methylornithine (alpha-DFMO) did not significantly inhibit epidermal keratinocyte proliferation at 5 X 10(-3) to 10(-4) M concentrations. At these doses, only alpha-DFMO was seen to decrease (by 70%) the cellular levels of putrescine, but not of spermidine or spermine. Epidermal keratinocyte growth in the higher dose of 20 mM alpha-DFMO, however, did not decrease the cellular levels of putrescine. Polyamine analyses of the spent medium showed that growth in 10 mM alpha-DFMO decreased the normal epidermal cell transport of putrescine and spermidine into the medium. At 20 mM alpha-DFMO concentration, the keratinocytes actually transported, intracellularly, the putrescine and spermidine that are naturally found in the foetal bovine component of the growth medium. We conclude from these studies that epidermal keratinocyte polyamine levels are determined by both the rate of synthesis, and of the transport of these amines into the extracellular medium. Since epidermal keratinocytes actively maintain specific polyamine levels, it appears that these molecules are essential for epidermal keratinocyte function.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 5","pages":"493-504"},"PeriodicalIF":0.0,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17257879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid cell cycle analysis. II. Phase durations and dispersions from computer analysis of RC curves. 快速细胞周期分析。2计算机分析RC曲线的相位持续时间和色散。

Cell and tissue kinetics Pub Date : 1983-09-01

J W Gray, E Bogart, D T Gavel, Y S George, D H Moore

{"title":"Rapid cell cycle analysis. II. Phase durations and dispersions from computer analysis of RC curves.","authors":"J W Gray, E Bogart, D T Gavel, Y S George, D H Moore","doi":"","DOIUrl":"","url":null,"abstract":"In this paper, we present a procedure for the rapid, quantitative estimation of the G1, S, and G2 + M phase durations and dispersions and the growth fraction for asynchronously growing cell populations. In this procedure, the cell population is pulse-labelled with a radioactive DNA precursor at the beginning of the analysis and then sampled periodically. The samples are dispersed, stained with a DNA specific dye, and processed through a cell sorter where cells from mid-S phase and G1 phase are sorted. The radioactivity per cell (RC) is determined for each sorted sample. In addition, the variation in the rate of incorporation of the radioactive DNA precursor across S phase is determined and the fractions of cells in the G1, S, and G2 + M phase are estimated from DNA distributions measured during sorting. We also describe an automatic computer analysis procedure for estimation of the G1, S, and G2 + M phase durations and dispersions and growth fraction by simultaneous analysis of the variations with time in the radioactivity per cell in G1 (RCG1) and radioactivity per cell in mid-S phase (RCS) curves, the G1, S, and G2 + M phase fractions and the variation in the rate of incorporation of radioactive DNA precursor uptake across S phase. The experimental and analytical aspects of the RC procedure are applied in the cell cycle analysis of Chinese hamster M3-1 cells grown in vitro. The parameters estimated by RC analysis agree well with similar parameters estimated by fraction-of-labelled-mitoses analysis.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 5","pages":"457-71"},"PeriodicalIF":0.0,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17933509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The percentage labelled mitoses technique shows the mean cell cycle time to be half its true value in Carcinoma NT. II. [3H]deoxyuridine studies. 百分比标记有丝分裂技术显示NT癌的平均细胞周期时间为其真实值的一半。[3 h]脱氧尿苷的研究。

Cell and tissue kinetics Pub Date : 1983-09-01

E Hamilton, J Dobbin

引用次数: 0

Summary of the Ninth International Cell Cycle Conference, 12-14 May 1982 at San Antonio, Texas. Abstracts. 第九届国际细胞周期会议总结，1982年5月12日至14日在德克萨斯州圣安东尼奥举行。摘要。

Cell and tissue kinetics Pub Date : 1983-09-01

引用次数: 0

Rat serum factors inhibiting the G1-S transition in hepatocytes. II. Properties of the low molecular weight factor. 抑制肝细胞G1-S转化的血清因子。2低分子量因子的性质。

Cell and tissue kinetics Pub Date : 1983-07-01

E Le Rumeur, J J Winchenne, G A Boffa, C Nadal

引用次数: 0

In vivo studies on the regeneration kinetics of enriched populations of haemopoietic spleen colony-forming cells from normal bone marrow. 正常骨髓造血脾脏集落形成细胞富集群体再生动力学的体内研究。

Cell and tissue kinetics Pub Date : 1983-07-01

J W Visser, J F Eliason

引用次数: 0

In vivo studies on the regeneration kinetics of haemopoietic spleen colony-forming cells from long-term bone marrow cultures. 长期骨髓培养的造血脾脏集落形成细胞再生动力学的体内研究。

Cell and tissue kinetics Pub Date : 1983-07-01

J F Eliason, M Edelstein

{"title":"In vivo studies on the regeneration kinetics of haemopoietic spleen colony-forming cells from long-term bone marrow cultures.","authors":"J F Eliason, M Edelstein","doi":"","DOIUrl":"","url":null,"abstract":"Production of murine haemopoietic progenitor cells, capable of forming colonies on the spleens of irradiated recipients (CFU-s), can be maintained in vitro for up to several months. We have examined the properties of the suspension (SUSP) and adherent (ADH) CFU-s from these long-term marrow cell cultures in the spleen colony assay system. The numbers of colonies formed was linear with the numbers of SUSP or ADH cells injected, although the concentrations were only 20-25% of that found in normal bone marrow (NBM). The seeding efficiencies for SUSP and ADH CFU-s, measured in spleen and femur 24 hr after transplantation, were not significantly different from those for CFU-s from NBM. The results demonstrate that the CFU-s assay can give valid estimates of colony-forming cell numbers in the cultured cell populations. We therefore used the CFU-s assay to study the regeneration of SUSP, ADH and NBM CFU-s in spleen and femur at various times after transplantation. The growth of the cultured cells appeared to be delayed by 36-48 hr compared to the regeneration of CFU-s from NBM. Thereafter, ADH CFU-s grew at the same rate as NBM CFU-s, whereas the rate of SUSP CFU-s regeneration was slightly, but not significantly, slower. This similarity in growth rates indicates that the CFU-s from long-term marrow cultures have similar self-renewal probabilities to normal CFU-s.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 4","pages":"375-83"},"PeriodicalIF":0.0,"publicationDate":"1983-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17201498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mouse epidermal cell proliferation after a single application of dimethyl sulphoxide (DMSO). 二甲基亚砜(DMSO)单次应用后小鼠表皮细胞增殖。

Cell and tissue kinetics Pub Date : 1983-07-01

K Elgjo, O P Clausen

引用次数: 0

Kinetics of DNA synthesis in feeding-dependent and independent hepatocyte populations of rats after partial hepatectomy. 大鼠部分肝切除术后摄食依赖型和独立型肝细胞群DNA合成动力学。

Cell and tissue kinetics Pub Date : 1983-07-01

M Kallenbach, N O Roome, R Schulte-Hermann

{"title":"Kinetics of DNA synthesis in feeding-dependent and independent hepatocyte populations of rats after partial hepatectomy.","authors":"M Kallenbach, N O Roome, R Schulte-Hermann","doi":"","DOIUrl":"","url":null,"abstract":"The effects of food consumption on the kinetics of hepatic DNA synthesis after partial hepatectomy (PH) have been studied in rats. Short-term (4-24 hr) fasting before or after PH resulted in depression and/or delay of DNA synthesis on days 1, 2 and 3 of regeneration. This depression was found in hepatocytes and, to a lesser extent, in littoral cells. Re-feeding resulted in an increase of DNA synthesis within 3-8 hr. The results suggest that two different hepatocyte subpopulations exist in regenerating rat liver: one which proceeds to DNA synthesis without apparent exogenous signals, and another one which needs, in addition to the specific mitogenic action of PH, food intake as a secondary permissive signal in order to initiate DNA synthesis. In the latter population food consumption appears to be required at two different stages: (1) in G0 or the early pre-replicative phase (PRP); (2) in the late PRP 3-8 hr before initiation of DNA synthesis. In the latter stage dietary protein is needed, but no so in the former. The dependence on feeding in the late PRP increases relatively with time after PH. No evidence was found to suggest a different distribution of the two cell populations throughout the liver acinus. The findings support the hypothesis that the known effects of the light-dark rhythm on the timing of DNA synthesis after PH are mediated by the natural feeding rhythm of rats fed ad libitum. In addition they offer a means for improving the synchrony of hepatocyte proliferation in regenerating rat liver.","PeriodicalId":75682,"journal":{"name":"Cell and tissue kinetics","volume":"16 4","pages":"321-32"},"PeriodicalIF":0.0,"publicationDate":"1983-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17911545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0