Smart medicine最新文献

{"title":"LINC00973/DTX3L Axis Promotes Non-Small Cell Lung Cancer Progression and Serves as a Therapeutic Target.","authors":"Yanke Chen, Yu Qian, Jiayuan Shi, Yi Wang, Tingyu Fu, Shuting Meng, Maoye Wang, Min Fu, Jiahui Zhang, Xiaoxin Zhang, Runbi Ji, Jianmei Gu, Xu Zhang, Zhe-Sheng Chen, Xiuqin Ma, Xinjian Fang","doi":"10.1002/smmd.70029","DOIUrl":"10.1002/smmd.70029","url":null,"abstract":"<p><p>Long non-coding RNAs (lncRNAs) constitute a critical class of regulatory molecules involved in cancer biology and play pivotal roles in tumor initiation and progression. Nevertheless, the biological functions of many newly identified lncRNAs in non-small cell lung cancer (NSCLC), as well as their potential therapeutic relevance, remain insufficiently characterized. In this study, high-throughput sequencing analysis of paired NSCLC tumor tissues and adjacent non-tumorous samples revealed that LINC00973 is significantly upregulated in tumor specimens. Moreover, elevated LINC00973 expression was found to be closely associated with poor clinical outcomes in patients with NSCLC. Functional assays showed that LINC00973 knockdown inhibits NSCLC cell proliferation, migration, and invasion while inducing apoptosis, whereas overexpression produces opposite effects. These observations were confirmed in vivo, where LINC00973 depletion markedly suppressed tumor growth and metastasis. Mechanistically, LINC00973 interacts with and stabilizes deltex E3 ubiquitin ligase 3L (DTX3L), preventing its ubiquitination-mediated degradation and activating the AKT signaling pathway. Therapeutically, RGD-modified exosome-mediated delivery of LINC00973 siRNA significantly inhibited NSCLC progression in mouse models. Moreover, a synthetic biology-based strategy enabling hepatic production of exosomes carrying LINC00973-targeting siRNA achieved robust anti-tumor effects. Together, these findings establish LINC00973 as an oncogenic lncRNA that promotes NSCLC progression via DTX3L stabilization and highlight LINC00973 as a promising therapeutic target.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"5 1","pages":"e70029"},"PeriodicalIF":12.8,"publicationDate":"2026-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146183814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Insights Into NFIX-Mediated DNA Recognition and Transcriptional Regulation in Skeletal Muscle.","authors":"Ci Zhu, Shuang Liu, Xi Chen, Chengxiao Qin, Yueyu Wang, Chunchun Xue, Lingxing Li, Wenlan Du, Xin Chen, Xiaofeng Li, Jie Shen, He Song","doi":"10.1002/smmd.70027","DOIUrl":"10.1002/smmd.70027","url":null,"abstract":"<p><p>Skeletal muscle is essential for voluntary movement and exhibits a remarkable capacity for regeneration following injury. NFIX, a member of the Nuclear Factor I (NFI) family of transcription factors, plays a critical role in both skeletal muscle development and regeneration. Despite its emerging importance, the molecular basis of NFIX-mediated DNA recognition and transcriptional regulation in skeletal muscle remains poorly defined. Here, we demonstrate that NFIX promotes key cellular processes in skeletal muscle cells, as siRNA-mediated knockdown of NFIX significantly reduces cell proliferation, increases apoptosis, and impairs differentiation. Transcriptomic analysis revealed that NFIX regulates a network of genes involved in muscle metabolism, stress responses, and immune inflammatory responses. Biophysical characterization showed that NFIX exists as a monomer in solution and binds palindromic DNA with a 1:1 stoichiometry. A high-resolution crystal structure of the NFIX_DBD bound to palindromic DNA reveals a monomeric binding mode driven by base-specific recognition of the TGGCA motif. Mutations that disrupt key DNA-contacting residues abolished both DNA binding and transcriptional activation in luciferase reporter assays. Together, these findings define the molecular mechanism of NFIX-dependent gene regulation in skeletal muscle and establish a structural framework for its function, providing new insights into the potential therapeutic targeting of NFIX in muscle diseases.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"5 1","pages":"e70027"},"PeriodicalIF":12.8,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12854730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146108853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiome Dysbiosis is Associated With Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection and Disease Progression to HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis: A Cross-Sectional Study.","authors":"Lorena Abreu Fernandes, Ana Olivia de Souza, Youko Nukui, Rosa Maria Marcusso, Augusto César Penalva de Oliveira, Jorge Casseb, Patricia Bianca Clissa, Silas G Villas-Boas, Sabri Saeed Sanabani","doi":"10.1002/smmd.70024","DOIUrl":"10.1002/smmd.70024","url":null,"abstract":"<p><p>Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic neuroinflammatory disease. Given the established role of the gut-brain axis in other neurological diseases such as multiple sclerosis, the role of the gut microbiome in the pathogenesis of HAM/TSP remains a critical unexplored area. The aim of this study was to characterize alterations in the gut microbiome associated with HTLV-1 infection and its clinical stages. We performed a cross-sectional analysis of the gut microbiome from 112 Brazilian individuals, including 24 healthy controls and 88 HTLV-1-infected individuals at different disease stages: 38 HAM patients, 17 patients with intermediate syndromes, and 33 asymptomatic carriers. Fecal samples were collected and analyzed using Illumina MiSeq sequencing to assess bacterial composition and diversity. Functional analysis was performed to identify differentially enriched gene categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) modules. Significant dysbiosis was observed in HTLV-1-infected individuals, characterized by reduced bacterial diversity, an inverted Firmicutes/Bacteroidetes ratio, and specific changes in bacterial genera. Notably, HAM patients exhibited decreased <i>Faecalibacterium</i> and increased <i>Ruminococcus_g2</i> abundance. These associations should be interpreted with caution, as patient cohorts were significantly older and differed in sex distribution from healthy controls. Functional analysis revealed 13 differentially enriched gene categories and five KEGG modules that were more abundant in HAM patients, indicating alterations in metabolic processes. These findings provide the first comprehensive insight into gut microbiome changes associated with HTLV-1 infection and disease progression. This study provides the first comprehensive insight into gut microbiome changes associated with HTLV-1 infection and disease progression. The identified microbial signatures and functional alterations highlight potential diagnostic and therapeutic targets for HTLV-1-associated diseases, particularly HAM. These findings open new avenues for further research and clinical applications.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"5 1","pages":"e70024"},"PeriodicalIF":12.8,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12794673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145968007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Artificial Intelligence-Based Computer Vision Model for Human Sperm Concentration, Motility, and Kinematics Analysis.","authors":"Sahar Shahali, David Mortimer, Moira K O'Bryan, Robert McLachlan, Deirdre Zander-Fox, Klaus Ackermann, Gulfam Ahmad, Adrian Neild, Reza Nosrati","doi":"10.1002/smmd.70026","DOIUrl":"10.1002/smmd.70026","url":null,"abstract":"<p><p>Accurate assessment of sperm concentration and motility is critical for the diagnosis and management of male infertility. However, current methods, manual hemocytometer counting and commercial computer-aided sperm analysis (CASA) systems, are limited by labor intensity, human error, and variable performance under diverse sample conditions. Here, we present an artificial intelligence (AI)-driven computer vision tool for high-resolution, quantitative analysis of sperm motility and concentration. In a prospective study of 26 semen samples (22 patients, 4 donors), we benchmarked the AI model against manual tracking (using Fiji software) and a commercial CASA system (Hamilton Thorne IVOS II). Our method computed concentration and motility parameters, including straight-line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), linearity (LIN), amplitude of lateral head displacement (ALH_max), and beat cross frequency (BCF). Calibration using donor samples enabled accurate mapping of tracked sperm counts to concentrations. The AI tool presented a strong linear correlation with manual tracking (<i>R</i> ² = 0.93-0.98; Root Mean Square Error (RMSE) = 3.3-7.3 μm/s for VSL, VCL, VAP), and outperformed CASA in both accuracy and consistency across all motility parameters. Post-calibration, ALH_max and BCF estimates improved substantially, with a 30%-50% reduction in RMSE. Grading of sperm motility by the AI model aligned closely with manual classification, avoiding the systematic misclassification typically observed with CASA. Furthermore, the AI system exhibited higher repeatability and robustness across duplicate samples and variable imaging conditions, with deviations below ± 2%. These findings demonstrate that our AI-based tool offers a quantitative and reliable alternative to current semen analysis platforms, supporting improved fertility diagnostics and potentially a more informative treatment process.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"5 1","pages":"e70026"},"PeriodicalIF":12.8,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12794671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conductive Nerve Guidance Conduits Loaded With Adipose Mesenchymal Stem Cells for Peripheral Nerve Regeneration.","authors":"Hong Cheng, Yangnan Hu, Menghui Liao, Xinyi Pang, Hui Zhang, Meihan Yu, Bin Zhang, Yu Wang, Huan Wang, Tingting Liu, Renjie Chai","doi":"10.1002/smmd.70025","DOIUrl":"10.1002/smmd.70025","url":null,"abstract":"<p><p>Peripheral nerve injury (PNI) presents a significant clinical challenge due to the intrinsic limitations of nerve regeneration and poor functional recovery. Although nerve guidance conduits (NGCs) offer a promising alternative to autografts, their therapeutic efficacy is often constrained by insufficient bioactivity and electrical conductivity. To address these dual deficiencies, we engineered an electroactive living nerve conduit by integrating silk sericin (SS)-modified carbon nanotubes (SCNTs) with adipose-derived stem cells (ADSCs). The SCNTs serve as a conductive scaffold, whereas the ADSCs provide a sustained release of neurotrophic factors. This design creates a synergistic microenvironment to promote neuronal maturation and axonal regeneration. In an experimental rat model featuring a 10-mm sciatic nerve gap, ADSC/SCNT/RAM NGCs demonstrated regenerative performance comparable to autografts, facilitating axon connection and recovery of motor functions. Histological assessment revealed that the implanted ADSC/SCNT/RAM NGCs promoted the most extensive nerve and axon regeneration among all groups, as evidenced by the significantly higher counts of S100 calcium-binding protein B (S100-β)-positive cells (10,152 ± 986.00) and Neurofilament Protein 200 (NF200)-positive cells (11,517 ± 795.70). Corroborating these histological findings, functional analysis demonstrated that the ADSC/SCNT/RAM group achieved the highest sciatic nerve function index (SFI) at 12 weeks post-surgery (-58.06 ± 1.46), a value comparable to the Autograft group (-57.73 ± 1.80). This strategy proposes a promising tissue-engineered alternative to autografts for nerve repair.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"4 4","pages":"e70025"},"PeriodicalIF":12.8,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12697924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145758673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating Single-Cell and Spatial Transcriptomics Reveals NK Cell Subpopulations Associated With Immunotherapy for Melanoma.","authors":"Zhicheng Hu, Yongfei Chen, Hao Yang, Qiuming Pan, Hongrui Li, Shuting Li, Junxi Wang, Yudi Huang, Guanglong Huang, Shanqiang Qu","doi":"10.1002/smmd.70023","DOIUrl":"10.1002/smmd.70023","url":null,"abstract":"<p><p>Immune checkpoint inhibitors (ICI) have demonstrated prolonged efficacy in certain melanoma patients, yet a significant portion of patients do not experience clinical improvement, with the mechanisms underlying this resistance still not fully understood. Using established cell markers, we partitioned the single-cell transcriptome into clusters, finding a notable link between NK cells and patient response to immunotherapy. We further identified four distinct subpopulations of NK cells, profiling marker gene sets and unique biological functions associated with each subpopulation. This analysis provides insights into the trajectory of NK cell development and differentiation, along with identifying the transcription factors driving these processes. The study pinpointed NK cluster 01 as pivotal in influencing patient sensitivity and prognosis during immunotherapy. Single-cell transcriptome and spatial transcriptomics (ST) analysis revealed the proximity of NK cluster 01 cells to melanoma cells, hinting at a potential regulation of cell-cell interaction via the IFN-II signaling pathway network. ST analysis revealed the spatial arrangement and interaction of NK cluster 01 cells with melanoma cells. This study explores the feasibility of targeting NK cluster 01 cells with small molecule drugs via molecular docking, offering a promising approach to bolster the clinical utility of NK cell therapy. We comprehensively analyze the heterogeneity of NK cells within melanoma, elucidate the potential regulatory interactions between NK cells and other microenvironmental components, and establish a basis for the future clinical utilization of distinct NK cell subsets as therapeutic targets.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"4 4","pages":"e70023"},"PeriodicalIF":12.8,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12677594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145703185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-Integrated Porcine Small Intestine Decellularized Extracellular Matrix Hydrogel Microparticles for Wound Healing.","authors":"Shangrui Rao, Hongzheng Li, Letian Meng, Lijun Cai, Weijian Sun, Yuyang Zhang","doi":"10.1002/smmd.70022","DOIUrl":"10.1002/smmd.70022","url":null,"abstract":"<p><p>Decellularized materials show good prospects in wound healing. Current research focuses on developing advanced decellularized scaffolds and designing multifunctional structures to enhance tissue repair. In this study, a kind of polydopamine (PDA)-integrated decellularized porcine small intestine tissue (dSI) and alginate (ALG) composite hydrogel microparticles (PDA@dSI/ALG) are proposed for efficient wound healing. These PDA@dSI/ALG are prepared via microfluidic electrospray technology combined with ionic crosslinking, followed by PDA coating. The dSI is derived from porcine small intestine tissue and is rich in various nutrients, which can promote the repair of damaged tissues. Moreover, incorporating PDA imparts the microparticles with superior photothermal responsiveness under near-infrared irradiation, enabling efficient heat generation to eradicate bacteria. Overall, the PDA@dSI/ALG composite hydrogel microparticles combine extracellular matrix support with photothermal antibacterial function, underscoring their translational potential for future wound healing strategies.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"4 4","pages":"e70022"},"PeriodicalIF":12.8,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12631813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145590087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-Responsive Microcapsules Delivering Oxygen and Traditional Chinese Medicine for Wound Healing.","authors":"Baojie Wen, Danqing Huang, Chuanhui Song, Yi Chen, Yuanjin Zhao","doi":"10.1002/smmd.70021","DOIUrl":"10.1002/smmd.70021","url":null,"abstract":"<p><p>Hydrogel microparticles with different actives encapsulation have reliable efficacy in wound repair, and the challenge is to improve the active substance to improve the efficacy. Here, a novel ultrasonic responsive core-shell microcapsule delivery system is proposed, which can simultaneously load traditional Chinese medicine sonosensitizer and oxygen synergistic effect to promote the healing of infected wounds. The microcapsule has a core of oxygen-rich perfluorocarbon (PO) and a hydrogel shell with curcumin (PO/C-MC). Ultrasound can trigger the release of oxygen from perfluorocarbon and stimulate the sonodynamic effect of curcumin, which is not only an anti-inflammatory traditional Chinese medicine but also a sonosensitizer, thus realizing the synergistic treatment of traditional Chinese medicine and acoustic dynamics. Under the irradiation of low-intensity ultrasound, PO/C-MCs can effectively increase the oxygen concentration and enhance the antibacterial effect of sonodynamic therapy, synergizing with the anti-inflammatory effect of traditional Chinese medicine to promote the rapid healing of chronic infected wounds. The study demonstrated that ultrasound-responsive PO/C-MCs microcapsules significantly enhanced the healing process of infected wounds in mice. Given these beneficial characteristics, PO/C-MCs represent a promising therapeutic candidate with considerable potential for clinical application in managing chronic infected wounds.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"4 4","pages":"e70021"},"PeriodicalIF":12.8,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145552247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of Sex-Biased Expressed Genes in Osteoarthritis.","authors":"Cuicui Wang, Meng Shi, Tiandao Li, Shuang Liu, Liang Fang, Ming Xu, Bo Zhang, Jie Shen","doi":"10.1002/smmd.70019","DOIUrl":"10.1002/smmd.70019","url":null,"abstract":"<p><p>Human osteoarthritis (OA) displays sex-specific patterns in its clinical presentation. Key features of the disease-such as prevalence, age of onset, progression, and response to treatment-vary between males and females. These differences have been associated with sex hormones, as well as anatomical, biomechanical, and behavioral distinctions between the sexes. However, the underlying mechanisms driving these sex-specific disparities in OA pathogenesis remain largely unknown. In this study, we analyzed transcriptomic data from human knee articular cartilage to investigate sex-specific gene expression in articular chondrocytes. We identified genes that are uniquely or predominantly expressed in either males or females in healthy cartilage. Notably, many of these sex-biased genes were significantly dysregulated in osteoarthritic cartilage, particularly those with higher expression in females. Furthermore, female-specific OA genes may exert protective effects on cartilage degeneration, whereas male-specific OA genes could impair cartilage homeostasis. Our findings provide insights into the genetic regulation of OA and highlight the influence of sex on its molecular pathology.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"4 4","pages":"e70019"},"PeriodicalIF":12.8,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12503069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements and Future Perspectives of Microfluidic Technology in Pediatric Healthcare.","authors":"Xuting Zhang, Andong Liu, Yanke Wang, Chao Niu, Xing Rong, Chang Jia, Jia Sun, Shiyang Song, Lexiang Zhang, Fangfu Ye, Changmin Shao, Maoping Chu","doi":"10.1002/smmd.70018","DOIUrl":"10.1002/smmd.70018","url":null,"abstract":"<p><p>Due to the ability to precisely control and manipulate fluids at the microscale, microfluidics provides unmatched advantages such as reduced sample size, rapid analysis, and enhanced sensitivity. Microfluidic technology has emerged as a revolutionary approach in pediatric healthcare, offering innovative solutions for diagnostics, monitoring, and treatment. This review presents a comprehensive overview of the recent advancements and future directions of microfluidic technology in the field of pediatrics. We begin with a brief introduction of several types of microfluidic devices that are more common in the pediatric field. Then, the substantial advances in biomedical applications of microfluidics in pediatric healthcare are explored, encompassing diagnosis, research, and treatment. Finally, challenges and limitations such as material selection, device standardization, stability, and regulatory considerations are also discussed that must be addressed to increase the utilization of microfluidics in the pediatric clinical field. Overall, this review underscores the transformative potential of microfluidics to improve the quality of healthcare and outcomes for pediatric patients, while also highlighting the opportunities for future research and development in this burgeoning field.</p>","PeriodicalId":74816,"journal":{"name":"Smart medicine","volume":"4 3","pages":"e70018"},"PeriodicalIF":12.8,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12442622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}