Advances in Virology最新文献_第8页

Molecular Detection of Torque Teno Sus Virus and Coinfection with African Swine Fever Virus in Blood Samples of Pigs from Some Slaughterhouses in Nigeria 尼日利亚部分屠宰场猪血液样本中扭力猪瘟病毒及合并感染非洲猪瘟病毒的分子检测

IF 2.2

Advances in Virology Pub Date : 2016-10-19 DOI: 10.1155/2016/6341015

P. Luka, J. Erume, B. Yakubu, O. Owolodun, D. Shamaki, F. Mwiine

{"title":"Molecular Detection of Torque Teno Sus Virus and Coinfection with African Swine Fever Virus in Blood Samples of Pigs from Some Slaughterhouses in Nigeria","authors":"P. Luka, J. Erume, B. Yakubu, O. Owolodun, D. Shamaki, F. Mwiine","doi":"10.1155/2016/6341015","DOIUrl":"https://doi.org/10.1155/2016/6341015","url":null,"abstract":"Torque teno sus virus 1 (TTSuV1a/TTSuV1b) infection is present in pig herds worldwide. This study investigated the prevalence of TTSuV1a/TTSuV1b infections in domestic pigs from some slaughterhouses in Nigeria as well as coinfection with African swine fever virus (ASFV) and described the phylogeny in relation to global strains. One hundred and eighty-one (181) blood samples from four slaughterhouses were used for the study and viral nucleic acid detection was carried out by PCR. Comparative sequence analysis was carried out to infer phylogeny. The overall prevalence of TTSuV1a/b was 17.7%. Prevalence of individual genotypes was 10.5% and 7.2% for TTSuV1a and TTSuV1b, respectively. Coinfection of ASFV/TTSuV1a/b was 7.7% while that of TTSuV1a and TTSuV1b was 1.7%. ASFV alone was detected in 11.91% of the total samples. The Nigerian TTSuV1a and TTSuV1b shared a sequence identity of 91–100% and 95–100%, respectively, among each other. The ASFV sequences were 100% identical to members of genotype 1. This is the first report on the presence of TTSuV1a/b in domestic pigs in Nigeria and coinfection with ASFV. Although the prevalence of TTSuV1a/b in Nigeria was low, we recommend further studies to establish the trend and possible role in the pathogenesis of ASFV.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"45 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/6341015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64471802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Immunogenicity of RSV F DNA Vaccine in BALB/c Mice RSV F DNA疫苗在BALB/c小鼠中的免疫原性

IF 2.2

Advances in Virology Pub Date : 2016-09-05 DOI: 10.1155/2016/7971847

E. Eroğlu, Ankur Singh, S. Bawage, P. Tiwari, K. Vig, S. Pillai, V. Dennis, S. Singh

{"title":"Immunogenicity of RSV F DNA Vaccine in BALB/c Mice","authors":"E. Eroğlu, Ankur Singh, S. Bawage, P. Tiwari, K. Vig, S. Pillai, V. Dennis, S. Singh","doi":"10.1155/2016/7971847","DOIUrl":"https://doi.org/10.1155/2016/7971847","url":null,"abstract":"Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths among the infant and elderly populations worldwide. There is no vaccine or a less effective drug available against RSV infections. Natural RSV infection stimulates the Th1 immune response and activates the production of neutralizing antibodies, while earlier vaccine trials that used UV-inactivated RSV exacerbated the disease due to the activation of the allergic Th2 response. With a focus on Th1 immunity, we developed a DNA vaccine containing the native RSV fusion (RSV F) protein and studied its immune response in BALB/c mice. High levels of RSV specific antibodies were induced during subsequent immunizations. The serum antibodies were able to neutralize RSV in vitro. The RSV inhibition by sera was also shown by immunofluorescence analyses. Antibody response of the RSV F DNA vaccine showed a strong Th1 response. Also, sera from RSV F immunized and RSV infected mice reduced the RSV infection by 50% and 80%, respectively. Our data evidently showed that the RSV F DNA vaccine activated the Th1 biased immune response and led to the production of neutralizing antibodies, which is the desired immune response required for protection from RSV infections.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/7971847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64543335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Serological Survey of Hantavirus in Inhabitants from Tropical and Subtropical Areas of Brazil 巴西热带和亚热带地区居民汉坦病毒血清学调查

IF 2.2

Advances in Virology Pub Date : 2016-03-10 DOI: 10.1155/2016/8628949

Felipe Alves Morais, Alexandre Pereira, Aparecida Santo Pietro Pereira, Marcos Lazaro Moreli, Luís Marcelo Aranha Camargo, Marcello Schiavo Nardi, Cristina Farah Tófoli, J. Araujo, Lilia Mara Dutra, Tatiana Lopes Ometto, R. Hurtado, Fábio Carmona de Jesus Maués, Tiene Zingano Hinke, Sati Jaber Mahmud, Monica Correia Lima, Luiz Tadeu Moraes Figueiredo, Edison Luiz Durigon

{"title":"Serological Survey of Hantavirus in Inhabitants from Tropical and Subtropical Areas of Brazil","authors":"Felipe Alves Morais, Alexandre Pereira, Aparecida Santo Pietro Pereira, Marcos Lazaro Moreli, Luís Marcelo Aranha Camargo, Marcello Schiavo Nardi, Cristina Farah Tófoli, J. Araujo, Lilia Mara Dutra, Tatiana Lopes Ometto, R. Hurtado, Fábio Carmona de Jesus Maués, Tiene Zingano Hinke, Sati Jaber Mahmud, Monica Correia Lima, Luiz Tadeu Moraes Figueiredo, Edison Luiz Durigon","doi":"10.1155/2016/8628949","DOIUrl":"https://doi.org/10.1155/2016/8628949","url":null,"abstract":"Brazil has reported more than 1,600 cases of hantavirus cardiopulmonary syndrome (HPS) since 1993, with a 39% rate of reported fatalities. Using a recombinant nucleocapsid protein of Araraquara virus, we performed ELISA to detect IgG antibodies against hantavirus in human sera. The aim of this study was to analyze hantavirus antibody levels in inhabitants from a tropical area (Amazon region) in Rondônia state and a subtropical (Atlantic Rain Forest) region in São Paulo state, Brazil. A total of 1,310 serum samples were obtained between 2003 and 2008 and tested by IgG-ELISA, and 82 samples (6.2%), of which 62 were from the tropical area (5.8%) and 20 from the subtropical area (8.3%), tested positive. Higher levels of hantavirus antibody were observed in inhabitants of the populous subtropical areas compared with those from the tropical areas in Brazil.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/8628949","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64578333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cumulative Impact of HIV and Multiple Concurrent Human Papillomavirus Infections on the Risk of Cervical Dysplasia HIV和多种并发人乳头瘤病毒感染对宫颈发育不良风险的累积影响

IF 2.2

Advances in Virology Pub Date : 2016-02-22 DOI: 10.1155/2016/7310894

D. Adler, M. Wallace, T. Bennie, B. Abar, T. Meiring, A. Williamson, L. Bekker

引用次数: 17

Adapted Lethality: What We Can Learn from Guinea Pig-Adapted Ebola Virus Infection Model 适应性致死性:我们可以从豚鼠适应性埃博拉病毒感染模型中学到什么

IF 2.2

Advances in Virology Pub Date : 2016-02-18 DOI: 10.1155/2016/8059607

S. Cheresiz, E. A. Semenova, A. Chepurnov

引用次数: 10

Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis 禽传染性支气管炎的发病机制及诊断方法

IF 2.2

Advances in Virology Pub Date : 2016-02-03 DOI: 10.1155/2016/4621659

F. Bande, S. Arshad, A. Omar, M. Bejo, M. Abubakar, Y. Abba

{"title":"Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis","authors":"F. Bande, S. Arshad, A. Omar, M. Bejo, M. Abubakar, Y. Abba","doi":"10.1155/2016/4621659","DOIUrl":"https://doi.org/10.1155/2016/4621659","url":null,"abstract":"Infectious bronchitis (IB) is one of the major economically important poultry diseases distributed worldwide. It is caused by infectious bronchitis virus (IBV) and affects both galliform and nongalliform birds. Its economic impact includes decreased egg production and poor egg quality in layers, stunted growth, poor carcass weight, and mortality in broiler chickens. Although primarily affecting the respiratory tract, IBV demonstrates a wide range of tissues tropism, including the renal and reproductive systems. Thus, disease outcome may be influenced by the organ or tissue involved as well as pathotypes or strain of the infecting virus. Knowledge on the epidemiology of the prevalent IBV strains in a particular region is therefore important to guide control and preventions. Meanwhile previous diagnostic methods such as serology and virus isolations are less sensitive and time consuming, respectively; current methods, such as reverse transcription polymerase chain reaction (RT-PCR), Restriction Fragment Length Polymorphism (RFLP), and sequencing, offer highly sensitive, rapid, and accurate diagnostic results, thus enabling the genotyping of new viral strains within the shortest possible time. This review discusses aspects on pathogenesis and diagnostic methods for IBV infection.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/4621659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64389564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

Serological Investigation of Akabane Virus Infection in Cattle and Sheep in Nigeria 尼日利亚牛羊感染阿卡巴纳病毒的血清学调查

IF 2.2

Advances in Virology Pub Date : 2016-01-26 DOI: 10.1155/2016/2936082

D. Oluwayelu, C. Aiki-Raji, E. Umeh, S. Mustapha, Adebowale Adebiyi

{"title":"Serological Investigation of Akabane Virus Infection in Cattle and Sheep in Nigeria","authors":"D. Oluwayelu, C. Aiki-Raji, E. Umeh, S. Mustapha, Adebowale Adebiyi","doi":"10.1155/2016/2936082","DOIUrl":"https://doi.org/10.1155/2016/2936082","url":null,"abstract":"Akabane virus (AKAV) is recognized as an important pathogen that causes abortions and congenital malformations in ruminants. However, it has not received adequate attention in Nigeria. Therefore, in investigating this disease, serum samples from 184 (abattoir and farm) head of cattle and 184 intensively reared sheep from two states in southwest Nigeria were screened for antibodies against AKAV using enzyme-linked immunosorbent assay. An overall seropositivity of 70.1% (129/184) was obtained with antibodies being detectable in 73.8% of abattoir (trade) cattle and 40.0% in farm cattle, while 4.3% (8/184) seropositivity was observed in sheep. All the age groups of cattle tested had seropositive animals, 0-1 year (1/7, 14.3%), 2-3 years (17/34, 50.0%), 4-5 years (92/121, 76.0%), and >5 years (19/22, 86.4%), while in sheep only the age groups of 2-3 and 4-5 years showed seropositivity of 4.1% (4/97) and 8.2% (4/49), respectively. The detection of antibody-positive animals among unvaccinated cattle and sheep provides evidence of AKAV infection in Nigeria. These findings call for continuous monitoring of the disease among ruminants in order to ascertain the actual burden and increase awareness of the disease. This will facilitate early detection and aid the development of appropriate control measures against the disease in Nigeria.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/2936082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64305183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Exosomes in Human Immunodeficiency Virus Type I Pathogenesis: Threat or Opportunity? 人类免疫缺陷病毒I型发病机制中的外泌体:威胁还是机遇?

IF 2.2

Advances in Virology Pub Date : 2016-01-04 DOI: 10.1155/2016/9852494

Sin-Yeang Teow, Alif Che Nordin, S. A. Ali, A. Khoo

{"title":"Exosomes in Human Immunodeficiency Virus Type I Pathogenesis: Threat or Opportunity?","authors":"Sin-Yeang Teow, Alif Che Nordin, S. A. Ali, A. Khoo","doi":"10.1155/2016/9852494","DOIUrl":"https://doi.org/10.1155/2016/9852494","url":null,"abstract":"Nanometre-sized vesicles, also known as exosomes, are derived from endosomes of diverse cell types and present in multiple biological fluids. Depending on their cellular origins, the membrane-bound exosomes packed a variety of functional proteins and RNA species. These microvesicles are secreted into the extracellular space to facilitate intercellular communication. Collective findings demonstrated that exosomes from HIV-infected subjects share many commonalities with Human Immunodeficiency Virus Type I (HIV-1) particles in terms of proteomics and lipid profiles. These observations postulated that HIV-resembled exosomes may contribute to HIV pathogenesis. Interestingly, recent reports illustrated that exosomes from body fluids could inhibit HIV infection, which then bring up a new paradigm for HIV/AIDS therapy. Accumulative findings suggested that the cellular origin of exosomes may define their effects towards HIV-1. This review summarizes the two distinctive roles of exosomes in regulating HIV pathogenesis. We also highlighted several additional factors that govern the exosomal functions. Deeper understanding on how exosomes promote or abate HIV infection can significantly contribute to the development of new and potent antiviral therapeutic strategy and vaccine designs.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9852494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64644726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken. 与肉鸡死亡相关的禽白血病病毒的分离及宏基因组鉴定。

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-08-15 DOI: 10.1155/2016/9058403

Faruku Bande, Siti Suri Arshad, Abdul Rahman Omar

{"title":"Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken.","authors":"Faruku Bande, Siti Suri Arshad, Abdul Rahman Omar","doi":"10.1155/2016/9058403","DOIUrl":"https://doi.org/10.1155/2016/9058403","url":null,"abstract":"Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"9058403"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9058403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34419526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle. 牛乳头瘤病毒1型的分子和系统发育分析:伊拉克牛首次报道。

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-06-20 DOI: 10.1155/2016/2143024

Mohammed A Hamad, Ahmed M Al-Shammari, Shoni M Odisho, Nahi Y Yaseen

{"title":"Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle.","authors":"Mohammed A Hamad, Ahmed M Al-Shammari, Shoni M Odisho, Nahi Y Yaseen","doi":"10.1155/2016/2143024","DOIUrl":"https://doi.org/10.1155/2016/2143024","url":null,"abstract":"This study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef cattle. In the current study, 140 cutaneous papilloma specimens were collected from cattle in central Iraq. These samples were submitted to histopathological examination, PCR, and sequencing analysis. The histopathology revealed that the main lesion type among the specimens was fibropapilloma. BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. Phylogenetic analysis confirmed the presence of BPV-1 and showed that the origin of infection may be imported European cattle. Obtaining a complete E5 gene sequence enabled us to perform structural predictions. This study presents the first report of BPV-1 infection in the Iraqi cattle and contributes to extending the knowledge of the origin of the spread of this disease. The results of this study will aid in the development of appropriate control measures and therapeutic strategies. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"2143024"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/2143024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34731293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20