Advances in Virology最新文献_第8页

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates. NxTAG呼吸道病原体检测面板及其与xTAG快速v2呼吸道病毒检测面板和薄膜阵列呼吸检测面板检测鼻咽部吸入物呼吸道病原体和培养分离株猪/禽流感亚型的比较

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-08-29 DOI: 10.1155/2017/1324276

K H Chan, K K W To, P T W Li, T L Wong, R Zhang, K K H Chik, G Chan, C C Y Yip, H L Chen, I F N Hung, J F W Chan, K Y Yuen

{"title":"Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates.","authors":"K H Chan, K K W To, P T W Li, T L Wong, R Zhang, K K H Chik, G Chan, C C Y Yip, H L Chen, I F N Hung, J F W Chan, K Y Yuen","doi":"10.1155/2017/1324276","DOIUrl":"https://doi.org/10.1155/2017/1324276","url":null,"abstract":"This study evaluated a new multiplex kit, Luminex NxTAG Respiratory Pathogen Panel, for respiratory pathogens and compared it with xTAG RVP Fast v2 and FilmArray Respiratory Panel using nasopharyngeal aspirate specimens and culture isolates of different swine/avian-origin influenza A subtypes (H2N2, H5N1, H7N9, H5N6, and H9N2). NxTAG RPP gave sensitivity of 95.2%, specificity of 99.6%, PPV of 93.5%, and NPV of 99.7%. NxTAG RPP, xTAG RVP, and FilmArray RP had highly concordant performance among each other for the detection of respiratory pathogens. The mean analytic sensitivity (TCID50/ml) of NxTAG RPP, xTAG RVP, and FilmArray RP for detection of swine/avian-origin influenza A subtype isolates was 0.7, 41.8, and 0.8, respectively. All three multiplex assays correctly typed and genotyped the influenza viruses, except for NxTAG RRP that could not distinguish H3N2 from H3N2v. Further investigation should be performed if H3N2v is suspected to be the cause of disease. Sensitive and specific laboratory diagnosis of all influenza A viruses subtypes is especially essential in certain epidemic regions, such as Southeast Asia. The results of this study should help clinical laboratory professionals to be aware of the different performances of commercially available molecular multiplex RT-PCR assays that are commonly adopted in many clinical diagnostic laboratories.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2017 ","pages":"1324276"},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1324276","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Patterns of Human Respiratory Viruses and Lack of MERS-Coronavirus in Patients with Acute Upper Respiratory Tract Infections in Southwestern Province of Saudi Arabia. 沙特阿拉伯西南省急性上呼吸道感染患者呼吸道病毒分布与mers冠状病毒缺失

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-02-27 DOI: 10.1155/2017/4247853

Ahmed A Abdulhaq, Vinod Kumar Basode, Anwar M Hashem, Ahmed S Alshrari, Nassrin A Badroon, Ahmed M Hassan, Tagreed L Alsubhi, Yahia Solan, Saleh Ejeeli, Esam I Azhar

{"title":"Patterns of Human Respiratory Viruses and Lack of MERS-Coronavirus in Patients with Acute Upper Respiratory Tract Infections in Southwestern Province of Saudi Arabia.","authors":"Ahmed A Abdulhaq, Vinod Kumar Basode, Anwar M Hashem, Ahmed S Alshrari, Nassrin A Badroon, Ahmed M Hassan, Tagreed L Alsubhi, Yahia Solan, Saleh Ejeeli, Esam I Azhar","doi":"10.1155/2017/4247853","DOIUrl":"https://doi.org/10.1155/2017/4247853","url":null,"abstract":"We undertook enhanced surveillance of those presenting with respiratory symptoms at five healthcare centers by testing all symptomatic outpatients between November 2013 and January 2014 (winter time). Nasal swabs were collected from 182 patients and screened for MERS-CoV as well as other respiratory viruses using RT-PCR and multiplex microarray. A total of 75 (41.2%) of these patients had positive viral infection. MERS-CoV was not detected in any of the samples. Human rhinovirus (hRV) was the most detected pathogen (40.9%) followed by non-MERS-CoV human coronaviruses (19.3%), influenza (Flu) viruses (15.9%), and human respiratory syncytial virus (hRSV) (13.6%). Viruses differed markedly depending on age in which hRV, Flu A, and hCoV-OC43 were more prevalent in adults and RSV, hCoV-HKU1, and hCoV-NL63 were mostly restricted to children under the age of 15. Moreover, coinfection was not uncommon in this study, in which 17.3% of the infected patients had dual infections due to several combinations of viruses. Dual infections decreased with age and completely disappeared in people older than 45 years. Our study confirms that MERS-CoV is not common in the southwestern region of Saudi Arabia and shows high diversity and prevalence of other common respiratory viruses. This study also highlights the importance and contribution of enhanced surveillance systems for better infection control.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2017 ","pages":"4247853"},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/4247853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34860361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Herpes Simplex Virus Latency: The DNA Repair-Centered Pathway. 单纯疱疹病毒潜伏期:以DNA修复为中心的途径

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-02-01 DOI: 10.1155/2017/7028194

Jay C Brown

引用次数: 14

Molecular Detection of Torque Teno Sus Virus and Coinfection with African Swine Fever Virus in Blood Samples of Pigs from Some Slaughterhouses in Nigeria 尼日利亚部分屠宰场猪血液样本中扭力猪瘟病毒及合并感染非洲猪瘟病毒的分子检测

IF 2.2

Advances in Virology Pub Date : 2016-10-19 DOI: 10.1155/2016/6341015

P. Luka, J. Erume, B. Yakubu, O. Owolodun, D. Shamaki, F. Mwiine

{"title":"Molecular Detection of Torque Teno Sus Virus and Coinfection with African Swine Fever Virus in Blood Samples of Pigs from Some Slaughterhouses in Nigeria","authors":"P. Luka, J. Erume, B. Yakubu, O. Owolodun, D. Shamaki, F. Mwiine","doi":"10.1155/2016/6341015","DOIUrl":"https://doi.org/10.1155/2016/6341015","url":null,"abstract":"Torque teno sus virus 1 (TTSuV1a/TTSuV1b) infection is present in pig herds worldwide. This study investigated the prevalence of TTSuV1a/TTSuV1b infections in domestic pigs from some slaughterhouses in Nigeria as well as coinfection with African swine fever virus (ASFV) and described the phylogeny in relation to global strains. One hundred and eighty-one (181) blood samples from four slaughterhouses were used for the study and viral nucleic acid detection was carried out by PCR. Comparative sequence analysis was carried out to infer phylogeny. The overall prevalence of TTSuV1a/b was 17.7%. Prevalence of individual genotypes was 10.5% and 7.2% for TTSuV1a and TTSuV1b, respectively. Coinfection of ASFV/TTSuV1a/b was 7.7% while that of TTSuV1a and TTSuV1b was 1.7%. ASFV alone was detected in 11.91% of the total samples. The Nigerian TTSuV1a and TTSuV1b shared a sequence identity of 91–100% and 95–100%, respectively, among each other. The ASFV sequences were 100% identical to members of genotype 1. This is the first report on the presence of TTSuV1a/b in domestic pigs in Nigeria and coinfection with ASFV. Although the prevalence of TTSuV1a/b in Nigeria was low, we recommend further studies to establish the trend and possible role in the pathogenesis of ASFV.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"45 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/6341015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64471802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Immunogenicity of RSV F DNA Vaccine in BALB/c Mice RSV F DNA疫苗在BALB/c小鼠中的免疫原性

IF 2.2

Advances in Virology Pub Date : 2016-09-05 DOI: 10.1155/2016/7971847

E. Eroğlu, Ankur Singh, S. Bawage, P. Tiwari, K. Vig, S. Pillai, V. Dennis, S. Singh

{"title":"Immunogenicity of RSV F DNA Vaccine in BALB/c Mice","authors":"E. Eroğlu, Ankur Singh, S. Bawage, P. Tiwari, K. Vig, S. Pillai, V. Dennis, S. Singh","doi":"10.1155/2016/7971847","DOIUrl":"https://doi.org/10.1155/2016/7971847","url":null,"abstract":"Respiratory syncytial virus (RSV) causes severe acute lower respiratory tract disease leading to numerous hospitalizations and deaths among the infant and elderly populations worldwide. There is no vaccine or a less effective drug available against RSV infections. Natural RSV infection stimulates the Th1 immune response and activates the production of neutralizing antibodies, while earlier vaccine trials that used UV-inactivated RSV exacerbated the disease due to the activation of the allergic Th2 response. With a focus on Th1 immunity, we developed a DNA vaccine containing the native RSV fusion (RSV F) protein and studied its immune response in BALB/c mice. High levels of RSV specific antibodies were induced during subsequent immunizations. The serum antibodies were able to neutralize RSV in vitro. The RSV inhibition by sera was also shown by immunofluorescence analyses. Antibody response of the RSV F DNA vaccine showed a strong Th1 response. Also, sera from RSV F immunized and RSV infected mice reduced the RSV infection by 50% and 80%, respectively. Our data evidently showed that the RSV F DNA vaccine activated the Th1 biased immune response and led to the production of neutralizing antibodies, which is the desired immune response required for protection from RSV infections.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/7971847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64543335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Serological Survey of Hantavirus in Inhabitants from Tropical and Subtropical Areas of Brazil 巴西热带和亚热带地区居民汉坦病毒血清学调查

IF 2.2

Advances in Virology Pub Date : 2016-03-10 DOI: 10.1155/2016/8628949

Felipe Alves Morais, Alexandre Pereira, Aparecida Santo Pietro Pereira, Marcos Lazaro Moreli, Luís Marcelo Aranha Camargo, Marcello Schiavo Nardi, Cristina Farah Tófoli, J. Araujo, Lilia Mara Dutra, Tatiana Lopes Ometto, R. Hurtado, Fábio Carmona de Jesus Maués, Tiene Zingano Hinke, Sati Jaber Mahmud, Monica Correia Lima, Luiz Tadeu Moraes Figueiredo, Edison Luiz Durigon

{"title":"Serological Survey of Hantavirus in Inhabitants from Tropical and Subtropical Areas of Brazil","authors":"Felipe Alves Morais, Alexandre Pereira, Aparecida Santo Pietro Pereira, Marcos Lazaro Moreli, Luís Marcelo Aranha Camargo, Marcello Schiavo Nardi, Cristina Farah Tófoli, J. Araujo, Lilia Mara Dutra, Tatiana Lopes Ometto, R. Hurtado, Fábio Carmona de Jesus Maués, Tiene Zingano Hinke, Sati Jaber Mahmud, Monica Correia Lima, Luiz Tadeu Moraes Figueiredo, Edison Luiz Durigon","doi":"10.1155/2016/8628949","DOIUrl":"https://doi.org/10.1155/2016/8628949","url":null,"abstract":"Brazil has reported more than 1,600 cases of hantavirus cardiopulmonary syndrome (HPS) since 1993, with a 39% rate of reported fatalities. Using a recombinant nucleocapsid protein of Araraquara virus, we performed ELISA to detect IgG antibodies against hantavirus in human sera. The aim of this study was to analyze hantavirus antibody levels in inhabitants from a tropical area (Amazon region) in Rondônia state and a subtropical (Atlantic Rain Forest) region in São Paulo state, Brazil. A total of 1,310 serum samples were obtained between 2003 and 2008 and tested by IgG-ELISA, and 82 samples (6.2%), of which 62 were from the tropical area (5.8%) and 20 from the subtropical area (8.3%), tested positive. Higher levels of hantavirus antibody were observed in inhabitants of the populous subtropical areas compared with those from the tropical areas in Brazil.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/8628949","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64578333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cumulative Impact of HIV and Multiple Concurrent Human Papillomavirus Infections on the Risk of Cervical Dysplasia HIV和多种并发人乳头瘤病毒感染对宫颈发育不良风险的累积影响

IF 2.2

Advances in Virology Pub Date : 2016-02-22 DOI: 10.1155/2016/7310894

D. Adler, M. Wallace, T. Bennie, B. Abar, T. Meiring, A. Williamson, L. Bekker

引用次数: 17

Adapted Lethality: What We Can Learn from Guinea Pig-Adapted Ebola Virus Infection Model 适应性致死性:我们可以从豚鼠适应性埃博拉病毒感染模型中学到什么

IF 2.2

Advances in Virology Pub Date : 2016-02-18 DOI: 10.1155/2016/8059607

S. Cheresiz, E. A. Semenova, A. Chepurnov

引用次数: 10

Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis 禽传染性支气管炎的发病机制及诊断方法

IF 2.2

Advances in Virology Pub Date : 2016-02-03 DOI: 10.1155/2016/4621659

F. Bande, S. Arshad, A. Omar, M. Bejo, M. Abubakar, Y. Abba

{"title":"Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis","authors":"F. Bande, S. Arshad, A. Omar, M. Bejo, M. Abubakar, Y. Abba","doi":"10.1155/2016/4621659","DOIUrl":"https://doi.org/10.1155/2016/4621659","url":null,"abstract":"Infectious bronchitis (IB) is one of the major economically important poultry diseases distributed worldwide. It is caused by infectious bronchitis virus (IBV) and affects both galliform and nongalliform birds. Its economic impact includes decreased egg production and poor egg quality in layers, stunted growth, poor carcass weight, and mortality in broiler chickens. Although primarily affecting the respiratory tract, IBV demonstrates a wide range of tissues tropism, including the renal and reproductive systems. Thus, disease outcome may be influenced by the organ or tissue involved as well as pathotypes or strain of the infecting virus. Knowledge on the epidemiology of the prevalent IBV strains in a particular region is therefore important to guide control and preventions. Meanwhile previous diagnostic methods such as serology and virus isolations are less sensitive and time consuming, respectively; current methods, such as reverse transcription polymerase chain reaction (RT-PCR), Restriction Fragment Length Polymorphism (RFLP), and sequencing, offer highly sensitive, rapid, and accurate diagnostic results, thus enabling the genotyping of new viral strains within the shortest possible time. This review discusses aspects on pathogenesis and diagnostic methods for IBV infection.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/4621659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64389564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 89

Serological Investigation of Akabane Virus Infection in Cattle and Sheep in Nigeria 尼日利亚牛羊感染阿卡巴纳病毒的血清学调查

IF 2.2

Advances in Virology Pub Date : 2016-01-26 DOI: 10.1155/2016/2936082

D. Oluwayelu, C. Aiki-Raji, E. Umeh, S. Mustapha, Adebowale Adebiyi

{"title":"Serological Investigation of Akabane Virus Infection in Cattle and Sheep in Nigeria","authors":"D. Oluwayelu, C. Aiki-Raji, E. Umeh, S. Mustapha, Adebowale Adebiyi","doi":"10.1155/2016/2936082","DOIUrl":"https://doi.org/10.1155/2016/2936082","url":null,"abstract":"Akabane virus (AKAV) is recognized as an important pathogen that causes abortions and congenital malformations in ruminants. However, it has not received adequate attention in Nigeria. Therefore, in investigating this disease, serum samples from 184 (abattoir and farm) head of cattle and 184 intensively reared sheep from two states in southwest Nigeria were screened for antibodies against AKAV using enzyme-linked immunosorbent assay. An overall seropositivity of 70.1% (129/184) was obtained with antibodies being detectable in 73.8% of abattoir (trade) cattle and 40.0% in farm cattle, while 4.3% (8/184) seropositivity was observed in sheep. All the age groups of cattle tested had seropositive animals, 0-1 year (1/7, 14.3%), 2-3 years (17/34, 50.0%), 4-5 years (92/121, 76.0%), and >5 years (19/22, 86.4%), while in sheep only the age groups of 2-3 and 4-5 years showed seropositivity of 4.1% (4/97) and 8.2% (4/49), respectively. The detection of antibody-positive animals among unvaccinated cattle and sheep provides evidence of AKAV infection in Nigeria. These findings call for continuous monitoring of the disease among ruminants in order to ascertain the actual burden and increase awareness of the disease. This will facilitate early detection and aid the development of appropriate control measures against the disease in Nigeria.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/2936082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64305183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9