Advances in Virology最新文献_第9页

Exosomes in Human Immunodeficiency Virus Type I Pathogenesis: Threat or Opportunity? 人类免疫缺陷病毒I型发病机制中的外泌体:威胁还是机遇?

IF 2.2

Advances in Virology Pub Date : 2016-01-04 DOI: 10.1155/2016/9852494

Sin-Yeang Teow, Alif Che Nordin, S. A. Ali, A. Khoo

{"title":"Exosomes in Human Immunodeficiency Virus Type I Pathogenesis: Threat or Opportunity?","authors":"Sin-Yeang Teow, Alif Che Nordin, S. A. Ali, A. Khoo","doi":"10.1155/2016/9852494","DOIUrl":"https://doi.org/10.1155/2016/9852494","url":null,"abstract":"Nanometre-sized vesicles, also known as exosomes, are derived from endosomes of diverse cell types and present in multiple biological fluids. Depending on their cellular origins, the membrane-bound exosomes packed a variety of functional proteins and RNA species. These microvesicles are secreted into the extracellular space to facilitate intercellular communication. Collective findings demonstrated that exosomes from HIV-infected subjects share many commonalities with Human Immunodeficiency Virus Type I (HIV-1) particles in terms of proteomics and lipid profiles. These observations postulated that HIV-resembled exosomes may contribute to HIV pathogenesis. Interestingly, recent reports illustrated that exosomes from body fluids could inhibit HIV infection, which then bring up a new paradigm for HIV/AIDS therapy. Accumulative findings suggested that the cellular origin of exosomes may define their effects towards HIV-1. This review summarizes the two distinctive roles of exosomes in regulating HIV pathogenesis. We also highlighted several additional factors that govern the exosomal functions. Deeper understanding on how exosomes promote or abate HIV infection can significantly contribute to the development of new and potent antiviral therapeutic strategy and vaccine designs.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9852494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64644726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken. 与肉鸡死亡相关的禽白血病病毒的分离及宏基因组鉴定。

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-08-15 DOI: 10.1155/2016/9058403

Faruku Bande, Siti Suri Arshad, Abdul Rahman Omar

{"title":"Isolation and Metagenomic Identification of Avian Leukosis Virus Associated with Mortality in Broiler Chicken.","authors":"Faruku Bande, Siti Suri Arshad, Abdul Rahman Omar","doi":"10.1155/2016/9058403","DOIUrl":"https://doi.org/10.1155/2016/9058403","url":null,"abstract":"Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"9058403"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9058403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34419526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle. 牛乳头瘤病毒1型的分子和系统发育分析:伊拉克牛首次报道。

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-06-20 DOI: 10.1155/2016/2143024

Mohammed A Hamad, Ahmed M Al-Shammari, Shoni M Odisho, Nahi Y Yaseen

{"title":"Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle.","authors":"Mohammed A Hamad, Ahmed M Al-Shammari, Shoni M Odisho, Nahi Y Yaseen","doi":"10.1155/2016/2143024","DOIUrl":"https://doi.org/10.1155/2016/2143024","url":null,"abstract":"This study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef cattle. In the current study, 140 cutaneous papilloma specimens were collected from cattle in central Iraq. These samples were submitted to histopathological examination, PCR, and sequencing analysis. The histopathology revealed that the main lesion type among the specimens was fibropapilloma. BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. Phylogenetic analysis confirmed the presence of BPV-1 and showed that the origin of infection may be imported European cattle. Obtaining a complete E5 gene sequence enabled us to perform structural predictions. This study presents the first report of BPV-1 infection in the Iraqi cattle and contributes to extending the knowledge of the origin of the spread of this disease. The results of this study will aid in the development of appropriate control measures and therapeutic strategies. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"2143024"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/2143024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34731293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

A Cross-Study Biomarker Signature of Human Bronchial Epithelial Cells Infected with Respiratory Syncytial Virus. 呼吸道合胞病毒感染人支气管上皮细胞的交叉研究生物标志物特征

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-05-04 DOI: 10.1155/2016/3605302

Luiz Gustavo Gardinassi

{"title":"A Cross-Study Biomarker Signature of Human Bronchial Epithelial Cells Infected with Respiratory Syncytial Virus.","authors":"Luiz Gustavo Gardinassi","doi":"10.1155/2016/3605302","DOIUrl":"https://doi.org/10.1155/2016/3605302","url":null,"abstract":"Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in children, elderly, and immunocompromised individuals. Despite of advances in diagnosis and treatment, biomarkers of RSV infection are still unclear. To understand the host response and propose signatures of RSV infection, previous studies evaluated the transcriptional profile of the human bronchial epithelial cell line-BEAS-2B-infected with different strains of this virus. However, the evolution of statistical methods and functional analysis together with the large amount of expression data provide opportunities to uncover novel biomarkers of inflammation and infections. In view of those facts publicly available microarray datasets from RSV-infected BEAS-2B cells were analyzed with linear model-based statistics and the platform for functional analysis InnateDB. The results from those analyses argue for the reevaluation of previously reported transcription patterns and biological pathways in BEAS-2B cell lines infected with RSV. Importantly, this study revealed a biosignature constituted by genes such as ABCC4, ARMC8, BCLAF1, EZH1, FAM118A, FAM208B, FUS, HSPH1, KAZN, MAP3K2, N6AMT1, PRMT2, S100PBP, SERPINA1, TLK2, ZNF322, and ZNF337 which should be considered in the development of new molecular diagnosis tools. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"3605302"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/3605302","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34620742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. 利用细胞培养独立rt - PCR方法直接检测和鉴定尼日利亚健康儿童粪便中的肠道病毒

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-03-20 DOI: 10.1155/2016/1412838

Temitope Oluwasegun Cephas Faleye, Moses Olubusuyi Adewumi, Bamidele Atinuke Coker, Felix Yasha Nudamajo, Johnson Adekunle Adeniji

{"title":"Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay.","authors":"Temitope Oluwasegun Cephas Faleye, Moses Olubusuyi Adewumi, Bamidele Atinuke Coker, Felix Yasha Nudamajo, Johnson Adekunle Adeniji","doi":"10.1155/2016/1412838","DOIUrl":"https://doi.org/10.1155/2016/1412838","url":null,"abstract":"Recently, a cell-culture independent protocol for detection of enteroviruses from clinical specimen was recommended by the WHO for surveillance alongside the previously established protocols. Here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols. Faecal samples were collected from sixty apparently healthy children in Ibadan, Nigeria. Samples were resuspended in phosphate buffered saline, RNA was extracted, and the VP1 gene was amplified using WHO recommended RT-snPCR protocol. Amplicons were sequenced and sequences subjected to phylogenetic analysis. Fifteen (25%) of the 60 samples yielded the expected band size. Of the 15 amplicons sequenced, 12 were exploitable. The remaining 3 had electropherograms with multiple peaks and were unexploitable. Eleven of the 12 exploitable sequences were identified as Coxsackievirus A1 (CVA1), CVA3, CVA4, CVA8, CVA20, echovirus 32 (E32), enterovirus 71 (EV71), EVB80, and EVC99. Subsequently, the last exploitable sequence was identified as enterobacteriophage baseplate gene by nucleotide BLAST. The results of this study document the first description of molecular sequence data on CVA1, CVA8, and E32 strains present in Nigeria. The result further showed that species A enteroviruses were more commonly detected in the region when cell-culture bias is bypassed. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"1412838"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/1412838","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34469601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Vitamin D-Regulated MicroRNAs: Are They Protective Factors against Dengue Virus Infection? 维生素d调控的microrna:它们是登革热病毒感染的保护因子吗?

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-05-11 DOI: 10.1155/2016/1016840

John F Arboleda, Silvio Urcuqui-Inchima

引用次数: 21

Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication BHK-21细胞表达因子X促进低致病性流感病毒复制

IF 2.2

Advances in Virology Pub Date : 2015-12-31 DOI: 10.1155/2015/675921

S. Shahsavandi, M. Ebrahimi, S. Masoudi, H. Izadi

{"title":"Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication","authors":"S. Shahsavandi, M. Ebrahimi, S. Masoudi, H. Izadi","doi":"10.1155/2015/675921","DOIUrl":"https://doi.org/10.1155/2015/675921","url":null,"abstract":"A cDNA clone for factor 10 (FX) isolated from chicken embryo inserted into the mammalian cell expression vector pCDNA3.1 was transfected into the baby hamster kidney (BHK-21) cell line. The generated BHK-21 cells with inducible expression of FX were used to investigate the efficacy of the serine transmembrane protease to proteolytic activation of influenza virus hemagglutinin (HA) with monobasic cleavage site. Data showed that the BHK-21/FX stably expressed FX after ten serial passages. The cells could proteolytically cleave the HA of low pathogenic avian influenza virus at multiplicity of infection 0.01. Growth kinetics of the virus on BHK-21/FX, BHK-21, and MDCK cells were evaluated by titrations of virus particles in each culture supernatant. Efficient multicycle viral replication was markedly detected in the cell at subsequent passages. Virus titration demonstrated that BHK-21/FX cell supported high-titer growth of the virus in which the viral titer is comparable to the virus grown in BHK-21 or MDCK cells with TPCK-trypsin. The results indicate potential application for the BHK-21/FX in influenza virus replication procedure and related studies.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/675921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65090612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antiviral Activity of Resveratrol against Human and Animal Viruses 白藜芦醇对人、动物病毒的抗病毒活性研究

IF 2.2

Advances in Virology Pub Date : 2015-11-29 DOI: 10.1155/2015/184241

Y. Abba, H. Hassim, H. Hamzah, M. Noordin

引用次数: 124

Clinical Symptoms of Human Rotavirus Infection Observed in Children in Sokoto, Nigeria 尼日利亚索科托儿童轮状病毒感染的临床症状

IF 2.2

Advances in Virology Pub Date : 2015-11-25 DOI: 10.1155/2015/890957

B. Alkali, A. Daneji, A. A. Magaji, L. S. Bilbis

引用次数: 19

Evaluating Andrographolide as a Potent Inhibitor of NS3-4A Protease and Its Drug-Resistant Mutants Using In Silico Approaches 利用计算机方法评价穿心莲内酯作为NS3-4A蛋白酶的有效抑制剂及其耐药突变体

IF 2.2

Advances in Virology Pub Date : 2015-10-26 DOI: 10.1155/2015/972067

V. Chandramohan, Anubhav Kaphle, Mamatha Chekuri, Sindhu Gangarudraiah, Gowrishankar Bychapur Siddaiah

{"title":"Evaluating Andrographolide as a Potent Inhibitor of NS3-4A Protease and Its Drug-Resistant Mutants Using In Silico Approaches","authors":"V. Chandramohan, Anubhav Kaphle, Mamatha Chekuri, Sindhu Gangarudraiah, Gowrishankar Bychapur Siddaiah","doi":"10.1155/2015/972067","DOIUrl":"https://doi.org/10.1155/2015/972067","url":null,"abstract":"Current combination therapy of PEG-INF and ribavirin against the Hepatitis C Virus (HCV) genotype-1 infections is ineffective in maintaining sustained viral response in 50% of the infection cases. New compounds in the form of protease inhibitors can complement the combination therapy. Asunaprevir is new to the drug regiment as the NS3-4A protease inhibitor, but it is susceptible to two mutations, namely, R155K and D168A in the protein. Thus, in our study, we sought to evaluate Andrographolide, a labdane-diterpenoid from the Andrographis paniculata plant as an effective compound for inhibiting the NS3-4A protease as well as its concomitant drug-resistant mutants by using molecular docking and dynamic simulations. Our study shows that Andrographolide has best docking scores of −15.0862, −15.2322, and −13.9072 compared to those of Asunaprevir −3.7159, −2.6431, and −5.4149 with wild-type R155K and D168A mutants, respectively. Also, as shown in the MD simulations, the compound was good in binding the target proteins and maintains strong bonds causing very less to negligible perturbation in the protein backbone structures. Our results validate the susceptibility of Asunaprevir to protein variants as seen from our docking studies and trajectory period analysis. Therefore, from our study, we hope to add one more option in the drug regiment to tackle drug resistance in HCV infections.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2015-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/972067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64190137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33