Advances in Virology最新文献_第9页

The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels. 利用De Novo设计的多重属特异性引物面板研究鸟类样本中的病毒RNA多样性。

IF 2.2

Advances in Virology Pub Date : 2018-08-12 eCollection Date: 2018-01-01 DOI: 10.1155/2018/3248285

Andrey A Ayginin, Ekaterina V Pimkina, Alina D Matsvay, Anna S Speranskaya, Marina V Safonova, Ekaterina A Blinova, Ilya V Artyushin, Vladimir G Dedkov, German A Shipulin, Kamil Khafizov

{"title":"The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels.","authors":"Andrey A Ayginin, Ekaterina V Pimkina, Alina D Matsvay, Anna S Speranskaya, Marina V Safonova, Ekaterina A Blinova, Ilya V Artyushin, Vladimir G Dedkov, German A Shipulin, Kamil Khafizov","doi":"10.1155/2018/3248285","DOIUrl":"https://doi.org/10.1155/2018/3248285","url":null,"abstract":"Advances in the next generation sequencing (NGS) technologies have significantly increased our ability to detect new viral pathogens and systematically determine the spectrum of viruses prevalent in various biological samples. In addition, this approach has also helped in establishing the associations of viromes with many diseases. However, unlike the metagenomic studies using 16S rRNA for the detection of bacteria, it is impossible to create universal oligonucleotides to target all known and novel viruses, owing to their genomic diversity and variability. On the other hand, sequencing the entire genome is still expensive and has relatively low sensitivity for such applications. The existing approaches for the design of oligonucleotides for targeted enrichment are usually involved in the development of primers for the PCR-based detection of particular viral species or genera, but not for families or higher taxonomic orders. In this study, we have developed a computational pipeline for designing the oligonucleotides capable of covering a significant number of known viruses within various taxonomic orders, as well as their novel variants. We have subsequently designed a genus-specific oligonucleotide panel for targeted enrichment of viral nucleic acids in biological material and demonstrated the possibility of its application for virus detection in bird samples. We have tested our panel using a number of collected samples and have observed superior efficiency in the detection and identification of viral pathogens. Since a reliable, bioinformatics-based analytical method for the rapid identification of the sequences was crucial, an NGS-based data analysis module was developed in this study, and its functionality in the detection of novel viruses and analysis of virome diversity was demonstrated.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2018-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3248285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36439572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Alternanthera mosaic potexvirus: Several Features, Properties, and Application. Alternathera mosaic potexvirus：几个特征、特性和应用。

IF 2.2

Advances in Virology Pub Date : 2018-06-19 DOI: 10.1155/2018/1973705

Ekaterina Donchenko, Ekaterina Trifonova, Nikolai Nikitin, Joseph Atabekov, Olga Karpova

{"title":"Alternanthera mosaic potexvirus: Several Features, Properties, and Application.","authors":"Ekaterina Donchenko, Ekaterina Trifonova, Nikolai Nikitin, Joseph Atabekov, Olga Karpova","doi":"10.1155/2018/1973705","DOIUrl":"10.1155/2018/1973705","url":null,"abstract":"Alternanthera mosaic virus (AltMV) is a typical member of the Potexvirus genus in its morphology and genome structure; still it exhibits a number of unique features. They allow this virus to be considered a promising object for biotechnology. Virions and virus-like particles (VLPs) of AltMV are stable in a wide range of conditions, including sera of laboratory animals. AltMV VLPs can assemble at various pH and ionic strengths. Furthermore, AltMV virions and VLPs demonstrate high immunogenicity, enhancing the immune response to the target antigen thus offering the possibility of being used as potential adjuvants. Recently, for the first time for plant viruses, we showed the structural difference between morphologically similar viral and virus-like particles on AltMV virions and VLPs. In this review, we discuss the features of AltMV virions, AltMV VLP assembly, and their structure and properties, as well as the characteristics of AltMV isolates, host plants, infection symptoms, AltMV isolation and purification, genome structure, viral proteins, and AltMV-based vectors.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2018-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/1973705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36321233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of HIV-1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients. HIV+患者尿细胞外囊泡中HIV-1和人蛋白的检测

IF 2.2

Advances in Virology Pub Date : 2018-03-12 eCollection Date: 2018-01-01 DOI: 10.1155/2018/7863412

Samuel I Anyanwu, Akins Doherty, Michael D Powell, Chamberlain Obialo, Ming B Huang, Alexander Quarshie, Claudette Mitchell, Khalid Bashir, Gale W Newman

{"title":"Detection of HIV-1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients.","authors":"Samuel I Anyanwu, Akins Doherty, Michael D Powell, Chamberlain Obialo, Ming B Huang, Alexander Quarshie, Claudette Mitchell, Khalid Bashir, Gale W Newman","doi":"10.1155/2018/7863412","DOIUrl":"https://doi.org/10.1155/2018/7863412","url":null,"abstract":"Background: Extracellular vesicles (EVs) are membrane bound, secreted by cells, and detected in bodily fluids, including urine, and contain proteins, RNA, and DNA. Our goal was to identify HIV and human proteins (HPs) in urinary EVs from HIV+ patients and compare them to HIV- samples.Methods: Urine samples were collected from HIV+ (n = 35) and HIV- (n = 12) individuals. EVs were isolated by ultrafiltration and characterized using transmission electron microscopy, tandem mass spectrometry (LC/MS/MS), and nanoparticle tracking analysis (NTA). Western blots confirmed the presence of HIV proteins. Gene ontology (GO) analysis was performed using FunRich and HIV Human Interaction database (HHID).Results: EVs from urine were 30-400 nm in size. More EVs were in HIV+ patients, P < 0.05, by NTA. HIV+ samples had 14,475 HPs using LC/MS/MS, while only 111 were in HIV-. HPs in the EVs were of exosomal origin. LC/MS/MS showed all HIV+ samples contained at least one HIV protein. GO analysis showed differences in proteins between HIV+ and HIV- samples and more than 50% of the published HPs in the HHID interacted with EV HIV proteins.Conclusion: Differences in the proteomic profile of EVs from HIV+ versus HIV- samples were found. HIV and HPs in EVs could be used to detect infection and/or diagnose HIV disease syndromes.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2018-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/7863412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36067200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Questioning the Extreme Neurovirulence of Monkey B Virus (Macacine alphaherpesvirus 1). 质疑猴B病毒(机器α疱疹病毒1型)的极端神经毒力。

IF 2.2

Advances in Virology Pub Date : 2018-01-01 DOI: 10.1155/2018/5248420

R Eberle, L Jones-Engel

引用次数: 19

Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line. 两株马来西亚非常强毒传染性法氏囊病病毒在BGM-70细胞系上的适应和分子特性

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-11-05 DOI: 10.1155/2017/8359047

Nafi'u Lawal, Mohd Hair-Bejo, Siti Suri Arshad, Abdul Rahman Omar, Aini Ideris

{"title":"Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line.","authors":"Nafi'u Lawal, Mohd Hair-Bejo, Siti Suri Arshad, Abdul Rahman Omar, Aini Ideris","doi":"10.1155/2017/8359047","DOIUrl":"https://doi.org/10.1155/2017/8359047","url":null,"abstract":"Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 and UPM190 (also known as UPMB00/81 and UPM04/190, respectively) isolated from local IBD outbreaks were serially passaged 12 times (EP12) in specific pathogen free (SPF) chicken embryonated eggs (CEE) by chorioallantoic membrane (CAM) route. The EP12 isolate was further adapted and serially propagated in BGM-70 cell line up to 20 passages (P20). Characteristic cytopathic effects (CPEs) were subtly observed at P1 in both isolates 72 hours postinoculation (pi). The CPE became prominent at P5 with cell rounding, cytoplasmic vacuoles, granulation, and detachment from flask starting from day 3 pi, up to 7 days pi with titers of 109.50 TCID50/mL and log109.80 TCID50/mL for UPM0081 and UPM190, respectively. The CPE became subtle at P17 and disappeared by P18 and P19 for UPM0081 and UPM190, respectively. However, the presence of IBDV was confirmed by immunoperoxidase, immunofluorescence, and RT-PCR techniques. Phylogenetic analysis showed that these two isolates were of the vvIBDV. It appears that a single mutation of UPM190 and UPM0081 IBDV isolates at D279N could facilitate vvIBDV strain adaptability in CEE and BGM-70 cultures.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/8359047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35638289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2. 选择与包膜糖蛋白结合的噬菌体显示肽对登革热病毒血清型 2 具有抗病毒活性。

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-09-10 DOI: 10.1155/2017/1827341

Carolina de la Guardia, Mario Quijada, Ricardo Lleonart

{"title":"Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2.","authors":"Carolina de la Guardia, Mario Quijada, Ricardo Lleonart","doi":"10.1155/2017/1827341","DOIUrl":"10.1155/2017/1827341","url":null,"abstract":"Dengue virus is a growing public health threat that affects hundreds of million peoples every year and leave huge economic and social damage. The virus is transmitted by mosquitoes and the incidence of the disease is increasing, among other causes, due to the geographical expansion of the vector's range and the lack of effectiveness in public health interventions in most prevalent countries. So far, no highly effective vaccine or antiviral has been developed for this virus. Here we employed phage display technology to identify peptides able to block the DENV2. A random peptide library presented in M13 phages was screened with recombinant dengue envelope and its fragment domain III. After four rounds of panning, several binding peptides were identified, synthesized, and tested against the virus. Three peptides were able to block the infectivity of the virus while not being toxic to the target cells. Blind docking simulations were done to investigate the possible mode of binding, showing that all peptides appear to bind domain III of the protein and may be mostly stabilized by hydrophobic interactions. These results are relevant to the development of novel therapeutics against this important virus.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5610824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35552958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence and Molecular Characterization of Hepatitis E Virus from Swine in Eastern Cape, South Africa. 南非东开普省猪戊型肝炎病毒的发病率和分子特征

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-01-16 DOI: 10.1155/2017/1073253

Olusesan Adeyemi Adelabu, Benson Chuks Iweriebor, U U Nwodo, Larry Chikwelu Obi, Anthony Ifeanyi Okoh

{"title":"Incidence and Molecular Characterization of Hepatitis E Virus from Swine in Eastern Cape, South Africa.","authors":"Olusesan Adeyemi Adelabu, Benson Chuks Iweriebor, U U Nwodo, Larry Chikwelu Obi, Anthony Ifeanyi Okoh","doi":"10.1155/2017/1073253","DOIUrl":"10.1155/2017/1073253","url":null,"abstract":"Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented. Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, this paper reports on prevalence of hepatitis E virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province for the presence of HEV. Of the 160 faecal samples screened, only seven were positive (4.4%) for HEV. The nucleotide sequences analyses revealed the isolates as sharing 82% to 99% identities with other strains (KX896664, KX896665, KX896666, KX896667, KX896668, KX896669, and KX896670) from different regions of the world. We conclude that HEV is present among swine in the Eastern Cape Province, albeit in low incidence, and this does have public health implications. There is a need for maintenance of high hygienic standards in order to prevent human infections through swine faecal materials and appropriate cooking of pork is highly advised.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5278201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49053513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Status of Porcine circovirus Type 2 and Its Associated Diseases in Sub-Saharan Africa. 猪圆环病毒2型及其在撒哈拉以南非洲相关疾病的全球状况

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-03-12 DOI: 10.1155/2017/6807964

Kayode O Afolabi, Benson C Iweriebor, Anthony I Okoh, Larry C Obi

{"title":"Global Status of Porcine circovirus Type 2 and Its Associated Diseases in Sub-Saharan Africa.","authors":"Kayode O Afolabi, Benson C Iweriebor, Anthony I Okoh, Larry C Obi","doi":"10.1155/2017/6807964","DOIUrl":"https://doi.org/10.1155/2017/6807964","url":null,"abstract":"Globally, Porcine circovirus type 2 (PCV2) is a recognized viral pathogen of great economic value in pig farming. It is the major cause of ravaging postweaning multisystemic wasting syndrome (PMWS) and many other disease syndromes generally regarded as Porcine circovirus associated diseases (PCVAD) in Europe. PCV2 infections, specifically PMWS, had impacted huge economic loss on swine production at different regions of the world. It has been studied and reported at different parts of the globe including: North and South America, Europe, Asia, Oceania, Middle East, and the Caribbean. However, till date, this virus and its associated diseases have been grossly understudied in sub-Sahara African region and the entire continent at large. Two out of forty-nine, representing just about 4% of countries that make up sub-Sahara Africa presently, have limited records on reported cases and occurrence of the viral pathogen despite the ubiquitous nature of the virus. This review presents an overview of the discovery of Porcine circovirus and its associated diseases in global pig herds and emphasizes the latest trends in PCV2 vaccines and antiviral drugs development and the information gaps that exist on the occurrence of this important viral pathogen in swine herds of sub-Saharan Africa countries. This will serve as wake-up call for immediate and relevant actions by stakeholders in the region.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/6807964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34893522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates. NxTAG呼吸道病原体检测面板及其与xTAG快速v2呼吸道病毒检测面板和薄膜阵列呼吸检测面板检测鼻咽部吸入物呼吸道病原体和培养分离株猪/禽流感亚型的比较

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-08-29 DOI: 10.1155/2017/1324276

K H Chan, K K W To, P T W Li, T L Wong, R Zhang, K K H Chik, G Chan, C C Y Yip, H L Chen, I F N Hung, J F W Chan, K Y Yuen

{"title":"Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates.","authors":"K H Chan, K K W To, P T W Li, T L Wong, R Zhang, K K H Chik, G Chan, C C Y Yip, H L Chen, I F N Hung, J F W Chan, K Y Yuen","doi":"10.1155/2017/1324276","DOIUrl":"https://doi.org/10.1155/2017/1324276","url":null,"abstract":"This study evaluated a new multiplex kit, Luminex NxTAG Respiratory Pathogen Panel, for respiratory pathogens and compared it with xTAG RVP Fast v2 and FilmArray Respiratory Panel using nasopharyngeal aspirate specimens and culture isolates of different swine/avian-origin influenza A subtypes (H2N2, H5N1, H7N9, H5N6, and H9N2). NxTAG RPP gave sensitivity of 95.2%, specificity of 99.6%, PPV of 93.5%, and NPV of 99.7%. NxTAG RPP, xTAG RVP, and FilmArray RP had highly concordant performance among each other for the detection of respiratory pathogens. The mean analytic sensitivity (TCID50/ml) of NxTAG RPP, xTAG RVP, and FilmArray RP for detection of swine/avian-origin influenza A subtype isolates was 0.7, 41.8, and 0.8, respectively. All three multiplex assays correctly typed and genotyped the influenza viruses, except for NxTAG RRP that could not distinguish H3N2 from H3N2v. Further investigation should be performed if H3N2v is suspected to be the cause of disease. Sensitive and specific laboratory diagnosis of all influenza A viruses subtypes is especially essential in certain epidemic regions, such as Southeast Asia. The results of this study should help clinical laboratory professionals to be aware of the different performances of commercially available molecular multiplex RT-PCR assays that are commonly adopted in many clinical diagnostic laboratories.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/1324276","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Patterns of Human Respiratory Viruses and Lack of MERS-Coronavirus in Patients with Acute Upper Respiratory Tract Infections in Southwestern Province of Saudi Arabia. 沙特阿拉伯西南省急性上呼吸道感染患者呼吸道病毒分布与mers冠状病毒缺失

IF 2.2

Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-02-27 DOI: 10.1155/2017/4247853

Ahmed A Abdulhaq, Vinod Kumar Basode, Anwar M Hashem, Ahmed S Alshrari, Nassrin A Badroon, Ahmed M Hassan, Tagreed L Alsubhi, Yahia Solan, Saleh Ejeeli, Esam I Azhar

{"title":"Patterns of Human Respiratory Viruses and Lack of MERS-Coronavirus in Patients with Acute Upper Respiratory Tract Infections in Southwestern Province of Saudi Arabia.","authors":"Ahmed A Abdulhaq, Vinod Kumar Basode, Anwar M Hashem, Ahmed S Alshrari, Nassrin A Badroon, Ahmed M Hassan, Tagreed L Alsubhi, Yahia Solan, Saleh Ejeeli, Esam I Azhar","doi":"10.1155/2017/4247853","DOIUrl":"https://doi.org/10.1155/2017/4247853","url":null,"abstract":"We undertook enhanced surveillance of those presenting with respiratory symptoms at five healthcare centers by testing all symptomatic outpatients between November 2013 and January 2014 (winter time). Nasal swabs were collected from 182 patients and screened for MERS-CoV as well as other respiratory viruses using RT-PCR and multiplex microarray. A total of 75 (41.2%) of these patients had positive viral infection. MERS-CoV was not detected in any of the samples. Human rhinovirus (hRV) was the most detected pathogen (40.9%) followed by non-MERS-CoV human coronaviruses (19.3%), influenza (Flu) viruses (15.9%), and human respiratory syncytial virus (hRSV) (13.6%). Viruses differed markedly depending on age in which hRV, Flu A, and hCoV-OC43 were more prevalent in adults and RSV, hCoV-HKU1, and hCoV-NL63 were mostly restricted to children under the age of 15. Moreover, coinfection was not uncommon in this study, in which 17.3% of the infected patients had dual infections due to several combinations of viruses. Dual infections decreased with age and completely disappeared in people older than 45 years. Our study confirms that MERS-CoV is not common in the southwestern region of Saudi Arabia and shows high diversity and prevalence of other common respiratory viruses. This study also highlights the importance and contribution of enhanced surveillance systems for better infection control.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/4247853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34860361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24