Advances in Virology最新文献_第9页

A Cross-Study Biomarker Signature of Human Bronchial Epithelial Cells Infected with Respiratory Syncytial Virus. 呼吸道合胞病毒感染人支气管上皮细胞的交叉研究生物标志物特征

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-05-04 DOI: 10.1155/2016/3605302

Luiz Gustavo Gardinassi

{"title":"A Cross-Study Biomarker Signature of Human Bronchial Epithelial Cells Infected with Respiratory Syncytial Virus.","authors":"Luiz Gustavo Gardinassi","doi":"10.1155/2016/3605302","DOIUrl":"https://doi.org/10.1155/2016/3605302","url":null,"abstract":"Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in children, elderly, and immunocompromised individuals. Despite of advances in diagnosis and treatment, biomarkers of RSV infection are still unclear. To understand the host response and propose signatures of RSV infection, previous studies evaluated the transcriptional profile of the human bronchial epithelial cell line-BEAS-2B-infected with different strains of this virus. However, the evolution of statistical methods and functional analysis together with the large amount of expression data provide opportunities to uncover novel biomarkers of inflammation and infections. In view of those facts publicly available microarray datasets from RSV-infected BEAS-2B cells were analyzed with linear model-based statistics and the platform for functional analysis InnateDB. The results from those analyses argue for the reevaluation of previously reported transcription patterns and biological pathways in BEAS-2B cell lines infected with RSV. Importantly, this study revealed a biosignature constituted by genes such as ABCC4, ARMC8, BCLAF1, EZH1, FAM118A, FAM208B, FUS, HSPH1, KAZN, MAP3K2, N6AMT1, PRMT2, S100PBP, SERPINA1, TLK2, ZNF322, and ZNF337 which should be considered in the development of new molecular diagnosis tools. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"3605302"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/3605302","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34620742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. 利用细胞培养独立rt - PCR方法直接检测和鉴定尼日利亚健康儿童粪便中的肠道病毒

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-03-20 DOI: 10.1155/2016/1412838

Temitope Oluwasegun Cephas Faleye, Moses Olubusuyi Adewumi, Bamidele Atinuke Coker, Felix Yasha Nudamajo, Johnson Adekunle Adeniji

{"title":"Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay.","authors":"Temitope Oluwasegun Cephas Faleye, Moses Olubusuyi Adewumi, Bamidele Atinuke Coker, Felix Yasha Nudamajo, Johnson Adekunle Adeniji","doi":"10.1155/2016/1412838","DOIUrl":"https://doi.org/10.1155/2016/1412838","url":null,"abstract":"Recently, a cell-culture independent protocol for detection of enteroviruses from clinical specimen was recommended by the WHO for surveillance alongside the previously established protocols. Here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols. Faecal samples were collected from sixty apparently healthy children in Ibadan, Nigeria. Samples were resuspended in phosphate buffered saline, RNA was extracted, and the VP1 gene was amplified using WHO recommended RT-snPCR protocol. Amplicons were sequenced and sequences subjected to phylogenetic analysis. Fifteen (25%) of the 60 samples yielded the expected band size. Of the 15 amplicons sequenced, 12 were exploitable. The remaining 3 had electropherograms with multiple peaks and were unexploitable. Eleven of the 12 exploitable sequences were identified as Coxsackievirus A1 (CVA1), CVA3, CVA4, CVA8, CVA20, echovirus 32 (E32), enterovirus 71 (EV71), EVB80, and EVC99. Subsequently, the last exploitable sequence was identified as enterobacteriophage baseplate gene by nucleotide BLAST. The results of this study document the first description of molecular sequence data on CVA1, CVA8, and E32 strains present in Nigeria. The result further showed that species A enteroviruses were more commonly detected in the region when cell-culture bias is bypassed. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2016 ","pages":"1412838"},"PeriodicalIF":2.2,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/1412838","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34469601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Vitamin D-Regulated MicroRNAs: Are They Protective Factors against Dengue Virus Infection? 维生素d调控的microrna:它们是登革热病毒感染的保护因子吗?

IF 2.2

Advances in Virology Pub Date : 2016-01-01 Epub Date: 2016-05-11 DOI: 10.1155/2016/1016840

John F Arboleda, Silvio Urcuqui-Inchima

引用次数: 21

Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication BHK-21细胞表达因子X促进低致病性流感病毒复制

IF 2.2

Advances in Virology Pub Date : 2015-12-31 DOI: 10.1155/2015/675921

S. Shahsavandi, M. Ebrahimi, S. Masoudi, H. Izadi

{"title":"Expression of Factor X in BHK-21 Cells Promotes Low Pathogenic Influenza Viruses Replication","authors":"S. Shahsavandi, M. Ebrahimi, S. Masoudi, H. Izadi","doi":"10.1155/2015/675921","DOIUrl":"https://doi.org/10.1155/2015/675921","url":null,"abstract":"A cDNA clone for factor 10 (FX) isolated from chicken embryo inserted into the mammalian cell expression vector pCDNA3.1 was transfected into the baby hamster kidney (BHK-21) cell line. The generated BHK-21 cells with inducible expression of FX were used to investigate the efficacy of the serine transmembrane protease to proteolytic activation of influenza virus hemagglutinin (HA) with monobasic cleavage site. Data showed that the BHK-21/FX stably expressed FX after ten serial passages. The cells could proteolytically cleave the HA of low pathogenic avian influenza virus at multiplicity of infection 0.01. Growth kinetics of the virus on BHK-21/FX, BHK-21, and MDCK cells were evaluated by titrations of virus particles in each culture supernatant. Efficient multicycle viral replication was markedly detected in the cell at subsequent passages. Virus titration demonstrated that BHK-21/FX cell supported high-titer growth of the virus in which the viral titer is comparable to the virus grown in BHK-21 or MDCK cells with TPCK-trypsin. The results indicate potential application for the BHK-21/FX in influenza virus replication procedure and related studies.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/675921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65090612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antiviral Activity of Resveratrol against Human and Animal Viruses 白藜芦醇对人、动物病毒的抗病毒活性研究

IF 2.2

Advances in Virology Pub Date : 2015-11-29 DOI: 10.1155/2015/184241

Y. Abba, H. Hassim, H. Hamzah, M. Noordin

引用次数: 124

Clinical Symptoms of Human Rotavirus Infection Observed in Children in Sokoto, Nigeria 尼日利亚索科托儿童轮状病毒感染的临床症状

IF 2.2

Advances in Virology Pub Date : 2015-11-25 DOI: 10.1155/2015/890957

B. Alkali, A. Daneji, A. A. Magaji, L. S. Bilbis

引用次数: 19

Evaluating Andrographolide as a Potent Inhibitor of NS3-4A Protease and Its Drug-Resistant Mutants Using In Silico Approaches 利用计算机方法评价穿心莲内酯作为NS3-4A蛋白酶的有效抑制剂及其耐药突变体

IF 2.2

Advances in Virology Pub Date : 2015-10-26 DOI: 10.1155/2015/972067

V. Chandramohan, Anubhav Kaphle, Mamatha Chekuri, Sindhu Gangarudraiah, Gowrishankar Bychapur Siddaiah

{"title":"Evaluating Andrographolide as a Potent Inhibitor of NS3-4A Protease and Its Drug-Resistant Mutants Using In Silico Approaches","authors":"V. Chandramohan, Anubhav Kaphle, Mamatha Chekuri, Sindhu Gangarudraiah, Gowrishankar Bychapur Siddaiah","doi":"10.1155/2015/972067","DOIUrl":"https://doi.org/10.1155/2015/972067","url":null,"abstract":"Current combination therapy of PEG-INF and ribavirin against the Hepatitis C Virus (HCV) genotype-1 infections is ineffective in maintaining sustained viral response in 50% of the infection cases. New compounds in the form of protease inhibitors can complement the combination therapy. Asunaprevir is new to the drug regiment as the NS3-4A protease inhibitor, but it is susceptible to two mutations, namely, R155K and D168A in the protein. Thus, in our study, we sought to evaluate Andrographolide, a labdane-diterpenoid from the Andrographis paniculata plant as an effective compound for inhibiting the NS3-4A protease as well as its concomitant drug-resistant mutants by using molecular docking and dynamic simulations. Our study shows that Andrographolide has best docking scores of −15.0862, −15.2322, and −13.9072 compared to those of Asunaprevir −3.7159, −2.6431, and −5.4149 with wild-type R155K and D168A mutants, respectively. Also, as shown in the MD simulations, the compound was good in binding the target proteins and maintains strong bonds causing very less to negligible perturbation in the protein backbone structures. Our results validate the susceptibility of Asunaprevir to protein variants as seen from our docking studies and trajectory period analysis. Therefore, from our study, we hope to add one more option in the drug regiment to tackle drug resistance in HCV infections.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2015-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/972067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64190137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis 麻疹病毒:亚急性硬化性全脑炎潜在分子标志物M蛋白一级序列的鉴定

IF 2.2

Advances in Virology Pub Date : 2015-10-26 DOI: 10.1155/2015/769837

Hasan Kweder, M. Ainouze, J. Brunel, D. Gerlier, E. Manet, R. Buckland

{"title":"Measles Virus: Identification in the M Protein Primary Sequence of a Potential Molecular Marker for Subacute Sclerosing Panencephalitis","authors":"Hasan Kweder, M. Ainouze, J. Brunel, D. Gerlier, E. Manet, R. Buckland","doi":"10.1155/2015/769837","DOIUrl":"https://doi.org/10.1155/2015/769837","url":null,"abstract":"Subacute Sclerosing Panencephalitis (SSPE), a rare lethal disease of children and young adults due to persistence of measles virus (MeV) in the brain, is caused by wild type (wt) MeV. Why MeV vaccine strains never cause SSPE is completely unknown. Hypothesizing that this phenotypic difference could potentially be represented by a molecular marker, we compared glycoprotein and matrix (M) genes from SSPE cases with those from the Moraten vaccine strain, searching for differential structural motifs. We observed that all known SSPE viruses have residues P64, E89, and A209 (PEA) in their M proteins whereas the equivalent residues for vaccine strains are either S64, K89, and T209 (SKT) as in Moraten or PKT. Through the construction of MeV recombinants, we have obtained evidence that the wt MeV-M protein PEA motif, in particular A209, is linked to increased viral spread. Importantly, for the 10 wt genotypes (of 23) that have had their M proteins sequenced, 9 have the PEA motif, the exception being B3, which has PET. Interestingly, cases of SSPE caused by genotype B3 have yet to be reported. In conclusion, our results strongly suggest that the PEA motif is a molecular marker for wt MeV at risk to cause SSPE.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2015-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/769837","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65144763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Seroprevalence of Herpes Simplex Virus Infection in HIV Coinfected Individuals in Eastern India with Risk Factor Analysis 印度东部HIV合并感染者血清单纯疱疹病毒感染的患病率及危险因素分析

IF 2.2

Advances in Virology Pub Date : 2015-10-19 DOI: 10.1155/2015/537939

S. Nag, Soma Sarkar, Debprasad Chattopadhyay, S. Bhattacharya, R. Biswas, M. Sengupta

{"title":"Seroprevalence of Herpes Simplex Virus Infection in HIV Coinfected Individuals in Eastern India with Risk Factor Analysis","authors":"S. Nag, Soma Sarkar, Debprasad Chattopadhyay, S. Bhattacharya, R. Biswas, M. Sengupta","doi":"10.1155/2015/537939","DOIUrl":"https://doi.org/10.1155/2015/537939","url":null,"abstract":"Herpes simplex virus type 2 (HSV-2) is the cause of most genital herpes while HSV-1 is responsible for orolabial and facial lesions. In immunocompromised individuals, like HIV patients, impaired immunity leads to more frequent symptomatic and asymptomatic HSV infection. Fifty-two blood samples from HIV patients with clinically diagnosed HSV infection were taken as cases, while 45 blood samples each from HIV-infected (HIV control) and noninfected patients without any herpetic lesion (non-HIV control) were taken as control. Serum was tested for IgM and IgG antibodies of both HSV-1 and HSV-2 by ELISA. The seroprevalence was compared among the three groups of study population, considering the demographic and socioeconomic parameters. The HSV-2 IgM was significantly higher (p < 0.005) in the HIV patient group (34.6%) than the HIV control (2.2%) and non-HIV control (2.2%) groups, whereas HSV-2 IgG seroprevalence was higher in both HIV patient (61.5%) and HIV control (57.8%) groups than the non-HIV control group (17.8%). The prevalence of HSV-2 was significantly higher in persons with multiple partners and in the reproductive age group. The overall seroprevalence of HSV-1 IgM was too low (<5%), whereas it was too high (about 90%) with HSV-1 IgG in all three study groups.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2015-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/537939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65023044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Nelfinavir impairs glycosylation of herpes simplex virus 1 envelope proteins and blocks virus maturation. 奈非那韦会损害单纯疱疹病毒1包膜蛋白的糖基化并阻止病毒成熟。

IF 2.2

Advances in Virology Pub Date : 2015-01-01 Epub Date: 2015-01-29 DOI: 10.1155/2015/687162

Soren Gantt, Eliora Gachelet, Jacquelyn Carlsson, Serge Barcy, Corey Casper, Michael Lagunoff

{"title":"Nelfinavir impairs glycosylation of herpes simplex virus 1 envelope proteins and blocks virus maturation.","authors":"Soren Gantt, Eliora Gachelet, Jacquelyn Carlsson, Serge Barcy, Corey Casper, Michael Lagunoff","doi":"10.1155/2015/687162","DOIUrl":"10.1155/2015/687162","url":null,"abstract":"Nelfinavir (NFV) is an HIV-1 aspartyl protease inhibitor that has numerous effects on human cells, which impart attractive antitumor properties. NFV has also been shown to have in vitro inhibitory activity against human herpesviruses (HHVs). Given the apparent absence of an aspartyl protease encoded by HHVs, we investigated the mechanism of action of NFV herpes simplex virus type 1 (HSV-1) in cultured cells. Selection of HSV-1 resistance to NFV was not achieved despite multiple passages under drug pressure. NFV did not significantly affect the level of expression of late HSV-1 gene products. Normal numbers of viral particles appeared to be produced in NFV-treated cells by electron microscopy but remain within the cytoplasm more often than controls. NFV did not inhibit the activity of the HSV-1 serine protease nor could its antiviral activity be attributed to inhibition of Akt phosphorylation. NFV was found to decrease glycosylation of viral glycoproteins B and C and resulted in aberrant subcellular localization, consistent with induction of endoplasmic reticulum stress and the unfolded protein response by NFV. These results demonstrate that NFV causes alterations in HSV-1 glycoprotein maturation and egress and likely acts on one or more host cell functions that are important for HHV replication. ","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":"2015 ","pages":"687162"},"PeriodicalIF":2.2,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33079120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0