Advances in Virology最新文献_第7页

A Unique Trinucleotide-Bloc Mutation-Based Two SARS-CoV-2 Genotypes with Potential Pathogenic Impacts. 基于三核苷酸块突变的两种具有潜在致病影响的SARS-CoV-2基因型

IF 2.2

Advances in Virology Pub Date : 2022-07-19 eCollection Date: 2022-01-01 DOI: 10.1155/2022/5618222

Mustak Ibn Ayub

{"title":"A Unique Trinucleotide-Bloc Mutation-Based Two SARS-CoV-2 Genotypes with Potential Pathogenic Impacts.","authors":"Mustak Ibn Ayub","doi":"10.1155/2022/5618222","DOIUrl":"https://doi.org/10.1155/2022/5618222","url":null,"abstract":"SARS-CoV-2, the novel coronavirus behind the COVID-19 pandemic, is acquiring new mutations in its genome. Although some mutations provide benefits to the virus against human immune response, others may result in their reduced pathogenicity and virulence. By analyzing more than 3000 high-coverage, complete sequences deposited in the GISAID database up to April 2020, here I report the uniqueness of the 28881-28883: GGG > AAC trinucleotide-bloc mutation in the SARS-CoV-2 genome that results in two substrains, described here as SARS-CoV-2g (28881-28883: GGG genotype) and SARS-CoV-2a (28881-28883: AAC genotype). Computational analysis and literature review suggest that this bloc mutation would bring 203-204: RG (arginine-glycine)>KR (lysine-arginine) amino acid changes in the nucleocapsid (N) protein affecting the SR (serine-arginine)-rich motif of the protein, a critical region for the transcription of viral RNA and replication of the virus. Thus, 28881-28883: GGG > AAC bloc mutation is expected to modulate the pathogenicity of SARS-CoV-2. These analyses suggest that SARS-CoV-2 has evolved into SARS-CoV-2a affecting COVID-19 infectivity and severity. To confirm these assumptions, retrospective and prospective epidemiological studies should be conducted in different countries to understand the course of pathogenicity of SARS-CoV-2a and SARS-CoV-2g. Laboratory research should focus on the bloc mutation to understand its true impacts on the course of the pandemic. Potential drug and vaccine development should also keep the 28881-28883 region of the N protein under consideration.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":" ","pages":"5618222"},"PeriodicalIF":2.2,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40553509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Early Favipiravir Therapy in Hospitalised COVID-19 Patients. Favipiravir早期治疗在COVID-19住院患者中的疗效

IF 2.2

Advances in Virology Pub Date : 2022-06-29 eCollection Date: 2022-01-01 DOI: 10.1155/2022/9240941

Abdulrahman Tawfik, Abdulrahman Alzahrani, Sami Alharbi, Jamal Almitairi, Arwa Alzahrani, Mohammed Ali Alshehri, Mohammed S Aldughaim, Hani Alothaid

{"title":"Effectiveness of Early Favipiravir Therapy in Hospitalised COVID-19 Patients.","authors":"Abdulrahman Tawfik, Abdulrahman Alzahrani, Sami Alharbi, Jamal Almitairi, Arwa Alzahrani, Mohammed Ali Alshehri, Mohammed S Aldughaim, Hani Alothaid","doi":"10.1155/2022/9240941","DOIUrl":"https://doi.org/10.1155/2022/9240941","url":null,"abstract":"COVID-19 is a disease caused by a novel coronavirus with no specific, standard treatment. We investigated the clinical data of COVID-19 patients admitted to King Fahad Specialist Hospital (KFSH) in Buraydah by comparing the patients who were treated early with favipiravir (within 3 days of admission) to patients who were treated after three days of admission or not treated. 165 patients were confirmed with PCR tests and admitted to KFSH for treatment. Comorbidities contributed significantly to increasing the length of stay in hospital at 11.4 ± 0.8 days compared to patients with no comorbidities at 8.6 ± 0.9 days (p=0.041). A total of 103 patients were treated with favipiravir, and we found that early treatment with favipiravir (within 3 days) reduced the length of stay in hospital significantly (8.8 ± 1.4 days) compared to patients who were treated after 3 days (13.3 ± 4.6 days) (p=0.0015). Moreover, patients with comorbidities in both early and late treatment groups had significantly higher average lengths of stay in hospital (11.2 ± 0.9 days) compared to patients with no comorbidities (7.9 ± 0.7 days) (p=0.017). Interestingly, patients treated early with favipiravir (with comorbidities and without) stayed fewer days in hospital compared to those with late treatment (p=0.021; a difference of 4.5 ± 1.9 days; and p=0.018; a difference of 4.2 ± 1.7 days, respectively). In conclusion, our analysis indicates that early treatment with favipiravir can reduce the length of stay in hospital and improve clinical manifestations of COVID-19 patients.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":" ","pages":"9240941"},"PeriodicalIF":2.2,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40578232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Confirmatory Virucidal Activity of Ionised Active Water S-100® on the SARS-CoV-2 Virus. 离子活性水S-100®对SARS-CoV-2病毒的杀病毒活性验证

IF 2.2

Advances in Virology Pub Date : 2022-06-17 eCollection Date: 2022-01-01 DOI: 10.1155/2022/5995775

Nathalie Wurtz, Issam Hasni, Audrey Bancod, Bernard La Scola

引用次数: 1

The Anti-Cytokine Storm Activity of Quercetin Zinc and Vitamin C Complex. 槲皮素锌和维生素C复合物的抗细胞因子风暴活性。

IF 2.2

Advances in Virology Pub Date : 2022-06-08 eCollection Date: 2022-01-01 DOI: 10.1155/2022/1575605

Hayder B Sahib, Omer Abid Kathum, Rafal Shakeeb Alanee, Rehab A M Jawad, Ahmed Majeed Al-Shammari

{"title":"The Anti-Cytokine Storm Activity of Quercetin Zinc and Vitamin C Complex.","authors":"Hayder B Sahib, Omer Abid Kathum, Rafal Shakeeb Alanee, Rehab A M Jawad, Ahmed Majeed Al-Shammari","doi":"10.1155/2022/1575605","DOIUrl":"https://doi.org/10.1155/2022/1575605","url":null,"abstract":"Cytokine storm is one of the causative deaths in a patient with severe acute respiratory syndrome. This study aimed at evaluating the prophylaxis effect of quercetin complexes with zinc and buffered ascorbic acid upon cytokine storm induction in mice and identifying the complex's acute toxicity. Mice were randomly divided into three groups: group A, control group, received 0.9% normal saline; group B received 100 mg/kg of the complex one hour before lipopolysaccharide (LPS) administration; and group C received the LPS IP 5 mg/kg. Then, levels of interleukin 1 and interleukin 6 were measured in the serum, and lung and kidney tissues were investigated for any changes that may have happened. Thirty mice were used to investigate the acute toxicity; mice were distributed into six groups: one control group and five treated groups; then several serial dilutions from the complex have been prepared for different concentrations from 5 g/kg to 0.312 g/kg. The animals were observed for 14 days. The LD50 was deduced by the straight-line equation calculated from the dose-response curve. The results in this study showed that group A had no significant tissue change. LPS group C showed tissue damage in the lung and kidney, which significantly prevented by the pretreated complex in group B. Moreover, the complex's acute toxicity value (LD50) was 655 mg/kg. In conclusion, the complex has significantly ameliorated LPS-induced acute lung and kidney injury, largely through suppression of inflammation; the large lethal dose value may make the complex have a promising therapeutic effect in the prevention of cytokine storm.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":" ","pages":"1575605"},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40041571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Partial Analysis of the Capsid Protein (VP1) of Human Sapovirus Isolated from Children with Diarrhoea in Rural Communities of South Africa 南非农村腹泻儿童中分离的人类Sapovirus衣壳蛋白(VP1)的部分分析

IF 2.2

Advances in Virology Pub Date : 2022-06-02 DOI: 10.1155/2022/9928378

Mpho Magwalivha, J. K. Ngandu, A. Traoré, N. Potgieter

引用次数: 1

Impact of Thermal Pretreatment of Saliva on the RT-PCR Detection of SARS-CoV-2 唾液热预处理对SARS-CoV-2 RT-PCR检测的影响

IF 2.2

Advances in Virology Pub Date : 2022-06-01 DOI: 10.1155/2022/7442907

O. Morais, Manuel Rui Azevedo Alves, Paulo Fernandes

引用次数: 4

Comparative Performance Evaluation of FilmArray BioFire RP2.1 and MAScIR 2.0 Assays for SARS-CoV-2 Detection FilmArray BioFire RP2.1与MAScIR 2.0检测SARS-CoV-2的性能比较

IF 2.2

Advances in Virology Pub Date : 2022-06-01 DOI: 10.1155/2022/4510900

S. Tazi, H. Kabbaj, Jalila Zirar, Amal Zouaki, Ghizlane El Amin, Othman El Himeur, M. Seffar

{"title":"Comparative Performance Evaluation of FilmArray BioFire RP2.1 and MAScIR 2.0 Assays for SARS-CoV-2 Detection","authors":"S. Tazi, H. Kabbaj, Jalila Zirar, Amal Zouaki, Ghizlane El Amin, Othman El Himeur, M. Seffar","doi":"10.1155/2022/4510900","DOIUrl":"https://doi.org/10.1155/2022/4510900","url":null,"abstract":"Background RT-PCR is the gold standard for COVID-19 diagnosis, but the lack of standardization of assays, whose diagnostic performance may widely vary, complicates the interpretation of the discrepancies that may be encountered. Study design. We conducted a retrospective study over a ten-month period at the Central Laboratory of Virology of Ibn Sina University Hospital of Rabat. We included nasopharyngeal swabs, positive and negative for SARS-CoV-2 on FilmArray BioFire® Respiratory Panel 2.1 Plus, which were subjected to our laboratory's reference test, MAScIR SARS-CoV-2 M kit 2.0, initially or after a freeze-thaw cycle. The results were compared, and each discrepant sample with sufficient volume underwent the third test, using ARGENE® SARS-CoV-2 R-GENE kit. Results Of 80 SARS-CoV-2 negative samples on FilmArray, there were no discordant results, whereas of 80 SARS-CoV-2 positive samples on FilmArray, 21 had discordant results on MAScIR, and only 11 could be tested on ARGENE, revealing positive results in 6 cases. 12.7% and 76.5% correspond to the discordance rates for MAScIR (with one or both targets detected on FilmArray), while 14.3% and 100% correspond to those of ARGENE. As the estimated sensitivity and specificity of FilmArray, compared with MAScIR, were 100% and 79.2%, respectively, its lower limit of detection, and ARGENE assay results, made it difficult to distinguish between false positives on FilmArray and false negatives on MAScIR without further investigations. Conclusion The implementation of a new assay in our laboratory revealed discrepancies suggesting a lack of sensitivity of our laboratory's reference test, leading us consequently to retain the SARS-CoV-2 positive result of these discordant samples on FilmArray, regardless of the detection of one or both targets. Our study, which is, to our knowledge, the first comparing FilmArray RP2.1 and MAScIR 2.0 assays for SARS-CoV-2 detection, highlights the urgent need to standardize RT-PCR assays for COVID-19 diagnosis.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46257292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020 2020年埃塞俄比亚南部爆发的小反刍动物害虫病毒的分离和分子特征

IF 2.2

Advances in Virology Pub Date : 2022-05-29 DOI: 10.1155/2022/5329898

Abdeally Mohammed, T. R. Chibssa, Waktole Terfa, F. Aklilu, Delesa Damena, Redeat Belayneh, Menbere Kidane

{"title":"Isolation and Molecular Characterization of Peste des Petits Ruminants Virus from Outbreaks in Southern Ethiopia, 2020","authors":"Abdeally Mohammed, T. R. Chibssa, Waktole Terfa, F. Aklilu, Delesa Damena, Redeat Belayneh, Menbere Kidane","doi":"10.1155/2022/5329898","DOIUrl":"https://doi.org/10.1155/2022/5329898","url":null,"abstract":"Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs (n = 24) were collected from sheep (n = 7) and goats (n = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells expressing the signaling lymphocyte activation molecule (VDS) and screened using RT-qPCR. Positive samples were confirmed by conventional RT-PCR followed by sequencing of a partial nucleoprotein (N) gene segment. Results revealed that 54% (n = 13/24) of the tested samples were PPRV-positive Phylogenetic analysis revealed that the viruses belonged to lineage IV and lineage II. The lineage IV viruses were similar, although not identical, to other lineage IV viruses previously reported in Ethiopia and other East African countries while the lineage II viruses have been reported for the first time in Ethiopia showed a high nucleotide identity (99.06%) with the vaccine (Nigeria 75/1) that is currently used in Ethiopia for the prevention of PPR. Further investigations are therefore recommended in order to fully understand the true nature of the lineage II PPRVs in Ethiopia.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49464019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association of Septic Shock with Mortality in Hospitalized COVID-19 Patients in Wuhan, China 武汉COVID-19住院患者感染性休克与死亡率的关系

IF 2.2

Advances in Virology Pub Date : 2022-04-23 DOI: 10.1155/2022/3178283

Shaoqiu Chen, Zitong Gao, Ling Hu, Y. Zuo, Yuanyuan Fu, Meilin Wei, Emory Zitello, G. Huang, Youping Deng

{"title":"Association of Septic Shock with Mortality in Hospitalized COVID-19 Patients in Wuhan, China","authors":"Shaoqiu Chen, Zitong Gao, Ling Hu, Y. Zuo, Yuanyuan Fu, Meilin Wei, Emory Zitello, G. Huang, Youping Deng","doi":"10.1155/2022/3178283","DOIUrl":"https://doi.org/10.1155/2022/3178283","url":null,"abstract":"Purpose Septic shock is a severe complication of COVID-19 patients. We aim to identify risk factors associated with septic shock and mortality among COVID-19 patients. Methods A total of 212 COVID-19 confirmed patients in Wuhan were included in this retrospective study. Clinical outcomes were designated as nonseptic shock and septic shock. Log-rank test was conducted to determine any association with clinical progression. A prediction model was established using random forest. Results The mortality of septic shock and nonshock patients with COVID-19 was 96.7% (29/30) and 3.8% (7/182). Patients taking hypnotics had a much lower chance to develop septic shock (HR = 0.096, p=0.0014). By univariate logistic regression analysis, 40 risk factors were significantly associated with septic shock. Based on multiple regression analysis, eight risk factors were shown to be independent risk factors and these factors were then selected to build a model to predict septic shock with AUC = 0.956. These eight factors included disease severity (HR = 15, p < 0.001), age > 65 years (HR = 2.6, p=0.012), temperature > 39.1°C (HR = 2.9, p=0.047), white blood cell count > 10 × 10⁹ (HR = 6.9, p < 0.001), neutrophil count > 75 × 10⁹ (HR = 2.4, p=0.022), creatine kinase > 5 U/L (HR = 1.8, p=0.042), glucose > 6.1 mmol/L (HR = 7, p < 0.001), and lactate > 2 mmol/L (HR = 22, p < 0.001). Conclusions We found 40 risk factors were significantly associated with septic shock. The model contained eight independent factors that can accurately predict septic shock. The administration of hypnotics could potentially reduce the incidence of septic shock in COVID-19 patients.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42832709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Role of Apoptosis in HIV Pathogenesis 细胞凋亡在HIV发病中的作用

IF 2.2

Advances in Virology Pub Date : 2022-04-14 DOI: 10.1155/2022/8148119

Cyril Jabea Ekabe, Njinju Asaba Clinton, Eugene Kusi Agyei, Jules Kehbila

引用次数: 1