选择与包膜糖蛋白结合的噬菌体显示肽对登革热病毒血清型 2 具有抗病毒活性。

IF 1.1 Q4 VIROLOGY
Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-09-10 DOI:10.1155/2017/1827341
Carolina de la Guardia, Mario Quijada, Ricardo Lleonart
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引用次数: 0

摘要

登革热病毒是一种日益严重的公共卫生威胁,每年影响数亿人,造成巨大的经济和社会损失。该病毒由蚊子传播,由于病媒的地理分布范围扩大以及大多数流行国家的公共卫生干预措施缺乏有效性等原因,该疾病的发病率正在上升。迄今为止,还没有针对这种病毒的高效疫苗或抗病毒药物问世。在这里,我们采用噬菌体展示技术来鉴定能够阻断 DENV2 的多肽。我们用重组登革热包膜及其片段结构域 III 对 M13 噬菌体中的随机肽库进行了筛选。经过四轮筛选,确定了几种结合肽,并进行了合成和针对病毒的测试。有三种肽能够阻断病毒的感染性,同时对靶细胞没有毒性。为了研究可能的结合模式,我们进行了盲对接模拟,结果表明,所有肽似乎都能与蛋白质的结构域 III 结合,并可能主要通过疏水相互作用而稳定下来。这些结果与开发针对这种重要病毒的新型疗法息息相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2.

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2.

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2.

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2.

Dengue virus is a growing public health threat that affects hundreds of million peoples every year and leave huge economic and social damage. The virus is transmitted by mosquitoes and the incidence of the disease is increasing, among other causes, due to the geographical expansion of the vector's range and the lack of effectiveness in public health interventions in most prevalent countries. So far, no highly effective vaccine or antiviral has been developed for this virus. Here we employed phage display technology to identify peptides able to block the DENV2. A random peptide library presented in M13 phages was screened with recombinant dengue envelope and its fragment domain III. After four rounds of panning, several binding peptides were identified, synthesized, and tested against the virus. Three peptides were able to block the infectivity of the virus while not being toxic to the target cells. Blind docking simulations were done to investigate the possible mode of binding, showing that all peptides appear to bind domain III of the protein and may be mostly stabilized by hydrophobic interactions. These results are relevant to the development of novel therapeutics against this important virus.

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来源期刊
CiteScore
2.30
自引率
0.00%
发文量
23
审稿时长
22 weeks
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