NxTAG呼吸道病原体检测面板及其与xTAG快速v2呼吸道病毒检测面板和薄膜阵列呼吸检测面板检测鼻咽部吸入物呼吸道病原体和培养分离株猪/禽流感亚型的比较

IF 1.1 Q4 VIROLOGY
Advances in Virology Pub Date : 2017-01-01 Epub Date: 2017-08-29 DOI:10.1155/2017/1324276
K H Chan, K K W To, P T W Li, T L Wong, R Zhang, K K H Chik, G Chan, C C Y Yip, H L Chen, I F N Hung, J F W Chan, K Y Yuen
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引用次数: 11

摘要

本研究利用不同猪/禽源甲型流感亚型(H2N2、H5N1、H7N9、H5N6和H9N2)的鼻咽抽吸标本和培养分离物,评估了一种新的多重检测试剂盒Luminex NxTAG Respiratory pathogens Panel,并将其与xTAG RVP Fast v2和FilmArray Respiratory Panel进行了比较。NxTAG RPP的敏感性为95.2%,特异性为99.6%,PPV为93.5%,NPV为99.7%。NxTAG RPP、xTAG RVP和FilmArray RP在呼吸道病原体检测中的表现高度一致。NxTAG RPP、xTAG RVP和FilmArray RP检测猪源/禽源甲型流感亚型分离物的平均分析灵敏度(TCID50/ml)分别为0.7、41.8和0.8。除了NxTAG RRP不能区分H3N2和H3N2v外,所有三种多重试验都正确分型和基因分型流感病毒。如果怀疑H3N2v是致病原因,应进行进一步调查。在某些流行地区,如东南亚,对所有甲型流感病毒亚型进行敏感和特异性的实验室诊断尤为重要。这项研究的结果应该有助于临床实验室专业人员了解市售的分子多重RT-PCR检测的不同性能,这些检测通常被许多临床诊断实验室采用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of NxTAG Respiratory Pathogen Panel and Comparison with xTAG Respiratory Viral Panel Fast v2 and Film Array Respiratory Panel for Detecting Respiratory Pathogens in Nasopharyngeal Aspirates and Swine/Avian-Origin Influenza A Subtypes in Culture Isolates.

This study evaluated a new multiplex kit, Luminex NxTAG Respiratory Pathogen Panel, for respiratory pathogens and compared it with xTAG RVP Fast v2 and FilmArray Respiratory Panel using nasopharyngeal aspirate specimens and culture isolates of different swine/avian-origin influenza A subtypes (H2N2, H5N1, H7N9, H5N6, and H9N2). NxTAG RPP gave sensitivity of 95.2%, specificity of 99.6%, PPV of 93.5%, and NPV of 99.7%. NxTAG RPP, xTAG RVP, and FilmArray RP had highly concordant performance among each other for the detection of respiratory pathogens. The mean analytic sensitivity (TCID50/ml) of NxTAG RPP, xTAG RVP, and FilmArray RP for detection of swine/avian-origin influenza A subtype isolates was 0.7, 41.8, and 0.8, respectively. All three multiplex assays correctly typed and genotyped the influenza viruses, except for NxTAG RRP that could not distinguish H3N2 from H3N2v. Further investigation should be performed if H3N2v is suspected to be the cause of disease. Sensitive and specific laboratory diagnosis of all influenza A viruses subtypes is especially essential in certain epidemic regions, such as Southeast Asia. The results of this study should help clinical laboratory professionals to be aware of the different performances of commercially available molecular multiplex RT-PCR assays that are commonly adopted in many clinical diagnostic laboratories.

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来源期刊
CiteScore
2.30
自引率
0.00%
发文量
23
审稿时长
22 weeks
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