E. Golenkina, S. Galkina, G. Viryasova, G. Sud’ina
{"title":"The Pro-Oxidant Effect of Class A CpG ODNs on Human Neutrophils Includes Both Non-Specific Stimulation of ROS Production and Structurally Determined Induction of NO Synthesis","authors":"E. Golenkina, S. Galkina, G. Viryasova, G. Sud’ina","doi":"10.3390/oxygen3010002","DOIUrl":"https://doi.org/10.3390/oxygen3010002","url":null,"abstract":"Synthetic CpG oligonucleotides are promising components of immunomodulatory drugs for the treatment and prophylaxis of infectious diseases, cancers, and allergies. Phosphorothioate modification stabilizes these compounds, contributing to the achievement of a clinical effect, but at the same time changes their immunomodulatory properties. We used the diffusible fluorescent dye dihydroethidium and the non-diffusible 6-carboxy-2′,7′dihydrochlorofluorescein diacetate and cytochrome c probes to demonstrate that it is the phosphorothioate backbones that determine the pronounced nonspecific pro-oxidant effect of CpG ODN on neutrophils. At the same time, as was shown using diaminofluorescein diacetate, the potentiation of nitric oxide synthesis in these leucocytes by CpG ODN class A strictly depends on the presence of CpG motifs and a palindromic “hairpin”. The results obtained will contribute to a more complete understanding of the physiological action of therapeutic agents based on synthetic CpG oligonucleotides.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41883383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Savinova, T. Salimova, E. Proskurnina, I. V. Rodionov, O. Kraevaya, P. Troshin, L. Kameneva, E. Malinovskaya, O. Dolgikh, N. Veiko, S. Kostyuk
{"title":"Effect of Water-Soluble Chlorine-Containing Buckminsterfullerene Derivative on the Metabolism of Reactive Oxygen Species in Human Embryonic Lung Fibroblasts","authors":"E. Savinova, T. Salimova, E. Proskurnina, I. V. Rodionov, O. Kraevaya, P. Troshin, L. Kameneva, E. Malinovskaya, O. Dolgikh, N. Veiko, S. Kostyuk","doi":"10.3390/oxygen3010001","DOIUrl":"https://doi.org/10.3390/oxygen3010001","url":null,"abstract":"The development of novel biologically active nanopharmaceuticals is a topical problem of medicine. Water-soluble fullerene derivatives are of particular interest due to their ability to regulate intracellular metabolism of reactive oxygen species (ROS) by direct oxidation or effects on oxidative and signaling enzymes. Here, we studied the effect of a water-soluble chlorine-containing derivative of C60 fullerene on human embryonic lung fibroblasts. MTT tests, intracellular ROS visualization, detection of the ROS-associated gene and protein expression, repair, cell proliferation and cell cycle regulation, and quantitation of oxidative DNA damage were used. Within the first three hours after exposure, antioxidant effects were revealed. Later, oxidative damage appeared. Thus, the studied compound had an ambiguous effect on ROS metabolism associated with a switch in the regulatory effect, which must be taken into account when assessing its biological activity and toxicity.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48055733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manoel Rios, V. Reis, Susana Soares, D. Moreira-Gonçalves, R. Fernandes
{"title":"Pros and Cons of Two Methods of Anaerobic Alactic Energy Assessment in a High-Intensity CrossFit® Workout","authors":"Manoel Rios, V. Reis, Susana Soares, D. Moreira-Gonçalves, R. Fernandes","doi":"10.3390/oxygen2040042","DOIUrl":"https://doi.org/10.3390/oxygen2040042","url":null,"abstract":"The current study aimed to evidence the strengths and weaknesses of two indirect methods for assessing the anaerobic alactic contribution to a specific CrossFit® workout. Thirty experienced crossfitters performed the Fran workout at maximal intensity, and ventilatory data were collected during the recovery period using a telemetric portable gas analyser to assess the oxygen uptake (VO2) of the off-kinetics fast component (Anarecovery). The kinetics of maximal phosphocreatine splitting (AnaPCr) were determined based on the literature. No differences between the two methods were observed (31.4 ± 4.0 vs. 30.4 ± 4.1 kJ for Anarecovery and AnaPCr, respectively). Despite the existence of some caveats (e.g., errors derived from a delay at the onset of VO2 recovery and the assumption of given values in the concentration of phosphocreatine per kilogram of wet muscle, respectively) in both methods, the data indicate that they yield similar results and allow for estimations of alactic energy contribution from a short-duration and high intensity CrossFit® routine. The current data contributes to CrossFit® workout evaluations and training strategies, helping researchers to evaluate crossfitters more accurately. The advantage of the two methods used in the current study is that they are non-invasive, which differs greatly from muscle biopsies.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47873834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete Recovery of an Iatrogenic Venous Gaseous Embolism (VGE) Clinical Presentation Thanks to a Timely Hyperbaric Oxygen Therapy (HBOT) Treatment","authors":"V. Zanon, S. Magri, G. Bonera, E. Camporesi","doi":"10.3390/oxygen2040041","DOIUrl":"https://doi.org/10.3390/oxygen2040041","url":null,"abstract":"Gas embolism is a potentially fatal occurrence in which bubbles are introduced into the arterial/venous circulation. A multifocal ischemia could be the life-threatening scenario of that and occurring after diving-related activities, or pulmonary barotrauma, or even iatrogenic procedure capable to cause an accidental introduction of gas into the vasculature. The case here reported concerns the good outcome of one of these rare occurrences thanks to a prompt diagnosis and proper management of the ongoing gaseous lesion. Unfortunately, it remains ethically and technically complex to consider feasible Randomized Clinical Trials (RCTs) in hyperbaric medicine in general, and even more so in this type of eventuality. We are happy to share this further favorable result as we believe it is just thanks to growing documented clinical evidence that now anyone, if ever had to be suffering from gas embolism, in presence of a RCT would widely prefer to be included in the arm that can take advantage of oxygen hyperbaric (HBO) therapy.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43251016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lag Time Determinations in Beer Samples. Influence of Alcohol and PBN Concentration in EPR Spin Trapping Experiments","authors":"M. Porcu, A. Fadda, D. Sanna","doi":"10.3390/oxygen2040040","DOIUrl":"https://doi.org/10.3390/oxygen2040040","url":null,"abstract":"The beers’ lag time measured with the PBN (N-t-Butyl-α-phenylnitrone) spin trapping experiments coupled with EPR spectroscopy is related to their antioxidant capacity and shelf life. The effect on the lag time of the alcohol content of the beer (starting values, 9 and 13%) and PBN concentration (50 or 200 mM) was analyzed on three samples, a pilsner, a strong lager, and a blonde Ale heated at 60 °C to trigger the production of the 1-hydroxyethyl radical. The lag time was determined only for the strong lager beer, with the values depending on the experimental conditions, being higher when PBN had a lower concentration. The antioxidant activity of the three beers gave the following values: 0.9089 mL pilsner beer/mg DPPH, 0.8510 mL strong lager beer/mg DPPH, and 0.7184 mL blonde Ale beer/mg DPPH, indicating that the unsuccessful determination of the lag time was not due to stale beers. The EPR intensity of the PBN adduct after 150 min (I150) and the area under the curve (AUC) were also measured and related to the oxidative stability of beers. Further studies are needed to verify the influence of the heating temperature in samples that do not show a lag time at 60 °C.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41659656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Samrot, Sanjay Preeth Ram Singh, R. Deenadhayalan, Vinod Vincent Rajesh, Sathiyamoorthy Padmanaban, K. Radhakrishnan
{"title":"Nanoparticles, a Double-Edged Sword with Oxidant as Well as Antioxidant Properties—A Review","authors":"A. Samrot, Sanjay Preeth Ram Singh, R. Deenadhayalan, Vinod Vincent Rajesh, Sathiyamoorthy Padmanaban, K. Radhakrishnan","doi":"10.3390/oxygen2040039","DOIUrl":"https://doi.org/10.3390/oxygen2040039","url":null,"abstract":"The usage of nanoparticles became inevitable in medicine and other fields when it was found that they could be administered to hosts to act as oxidants or antioxidants. These oxidative nanoparticles act as pro-oxidants and induce oxidative stress-mediated toxicity through the generation of free radicals. Some nanoparticles can act as antioxidants to scavenge these free radicals and help in maintaining normal metabolism. The oxidant and antioxidant properties of nanoparticles rely on various factors including size, shape, chemical composition, etc. These properties also help them to be taken up by cells and lead to further interaction with cell organelles/biological macromolecules, leading to either the prevention of oxidative damage, the creation of mitochondrial dysfunction, damage to genetic material, or cytotoxic effects. It is important to know the properties that make these nanoparticles act as oxidants/antioxidants and the mechanisms behind them. In this review, the roles and mechanisms of nanoparticles as oxidants and antioxidants are explained.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49304700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Hancock, Grace Russell, T. Craig, J. May, H. Morse, J. Stamler
{"title":"Understanding Hydrogen: Lessons to Be Learned from Physical Interactions between the Inert Gases and the Globin Superfamily","authors":"J. Hancock, Grace Russell, T. Craig, J. May, H. Morse, J. Stamler","doi":"10.3390/oxygen2040038","DOIUrl":"https://doi.org/10.3390/oxygen2040038","url":null,"abstract":"Hydrogen gas (molecular hydrogen, H2) has significant effects in a range of organisms, from plants to humans. Many inert gases have been reported to have similar effects, and such responses may be most pronounced when cells are stressed. Xenon (Xe), for example, is a well-known anesthetic. The direct targets of these gases, in most cases, remain elusive. Myoglobin and hemoglobin are known for their roles in the transport of gases through coordinate interactions with metals (O2, NO, CO) and covalent modifications of thiols (NO, H2S) and amines (CO2). These are well exemplified in biotrophic reactions of NO with heme iron (to form iron nitrosyl heme) and cysteine (to form bioactive S-nitrosothiols) essential for tissue oxygenation. Here, we consider an alternative “third mode” of gas transport in what have been dubbed “Xenon pockets”, whereby inert gases may have functional effects. Many proteins have similar cavities, and possible effects include alterations in allosteric properties of proteins (potentially altering protein hydration). Here, it is suggested that similar to other inert gases, H2 also has biological effects by utilizing these protein structures. This ought to be investigated further, in a range of species, to determine if this is the mode of action of H2.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45304544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Rogers, Ashleigh M. Davis, Ashley E. Rice, C. Ballmann
{"title":"Effects of Acute Beetroot Juice Ingestion on Reactive Agility Performance","authors":"R. Rogers, Ashleigh M. Davis, Ashley E. Rice, C. Ballmann","doi":"10.3390/oxygen2040037","DOIUrl":"https://doi.org/10.3390/oxygen2040037","url":null,"abstract":"Beetroot juice (BRJ) is high in inorganic nitrate (NO3) which has been shown to enhance vascular function, cognition, and physical performance. Acute BRJ supplementation has been reported to enhance explosive resistance exercise performance and simple reaction time in diseased populations. However, it remains unknown if acute BRJ supplementation can enhance agility and reactive drills in healthy individuals, which are relevant to many sports. The purpose of this study was to investigate the effects of acute BRJ supplementation on simple reaction time and reactive agility performance. In a counterbalanced double-blinded manner, physically active males completed two trials each with a differing single-dose treatment: (1) Placebo (PL), (2) BRJ. Each treatment was consumed 2-hrs before experimental testing. Plasma Nitrate (NO3) and Nitrite (NO2) were measured via capillary blood sampling and colorimetric assay. Reaction time was assessed using a simple reaction time light test. Reactive agility was measured via a semi-circle drill and a get-up-and-go drill. All tests used FITLIGHT LED sensors to record response time. Each visit was separated by a 72-h washout period. Acute BRJ ingestion resulted in significantly greater plasma NO3 (p < 0.001) and NO2 (p = 0.008) compared to PL. BRJ significantly improved response time during the semi-circle drill (p = 0.011) and get-up and go drill (p = 0.027) compared to PL. No differences between treatments were observed for simple reaction time (p = 0.279). Collectively, these findings suggest that acute BRJ ingestion may improve reactive agility performance likely mediated by systemic increases in NO3/NO2. Future research is needed to investigate how these findings translate to game-play and sports competition.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42897028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oxidative Stress as an Underlying Mechanism of Bacteria-Inflicted Damage to Male Gametes","authors":"E. Tvrdá, F. Benko, M. Ďuračka","doi":"10.3390/oxygen2040036","DOIUrl":"https://doi.org/10.3390/oxygen2040036","url":null,"abstract":"Bacterial infestation of the male reproductive system with subsequent effects of bacteria on the structural integrity and functional activity of male gametes has become a significant factor in the etiology of male reproductive dysfunction. Bacteria may affect male fertility either by directly interacting with structures critical for sperm survival or indirectly by triggering a local immune response, leukocytospermia or reactive oxygen species (ROS) overproduction followed by oxidative stress development. This review aims to provide an overview of the currently available knowledge on bacteriospermia-associated sperm damage with a special emphasis on oxidative mechanisms underlying sperm deterioration caused by bacterial action. At the same time, we strive to summarize readily available alternatives to prevent or counteract alterations to spermatozoa caused by bacterial colonization of semen or by oxidative stress as an accompanying phenomenon of bacteriospermia.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48655274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vasileios M. Pappas, D. Palaiogiannis, V. Athanasiadis, Theodoros G. Chatzimitakos, Eleni Bozinou, D. Makris, S. Lalas
{"title":"Optimization of Pulsed Electric-Field-Based Total Polyphenols’ Extraction from Elaeagnus pungens ‘Limelight’ Leaves Using Hydroethanolic Mixtures","authors":"Vasileios M. Pappas, D. Palaiogiannis, V. Athanasiadis, Theodoros G. Chatzimitakos, Eleni Bozinou, D. Makris, S. Lalas","doi":"10.3390/oxygen2040035","DOIUrl":"https://doi.org/10.3390/oxygen2040035","url":null,"abstract":"In this study, the use of pulsed electric field (PEF) for the extraction of polyphenols from mature Elaeagnus pungens ‘Limelight’ leaves is discussed. Optimization of the main parameters that affect the extraction process was carried out. More specifically, the composition of the solvent (ethanol, water, and mixtures of the two at a 25% v/v step gradient) and the main PEF-related parameters (i.e., pulse duration, pulse period, and electric field intensity) was optimized. The obtained extracts were examined for their polyphenol content with the Folin–Ciocalteu assay and individual polyphenols were also assessed with high-performance liquid chromatography. The extracts obtained with PEF were compared to the extract compared without PEF, in terms of total polyphenols. According to the results, the optimum extraction parameters were found to be a pulse duration of 10 μs, a pulse period of 1000 μs, and an electric field intensity of 0.85 kV cm−1 after 20 min of extraction. The optimum solvent was found to be the 50% (v/v) ethanol/water mixture. The extract prepared under the optimum conditions was found to contain 58% more polyphenols compared with the extract prepared without PEF. Moreover, an increase of up to 92% was recorded for specific polyphenols. Based on the above, it was evidenced that the examined parameters influenced the recovery of polyphenols, suggesting that such parameters should be also examined in similar studies, in order to maximize the extraction yield of polyphenols.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45653883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}