Oxygen (Basel, Switzerland)最新文献_第3页

The Underexplored Landscape of Hypoxia-Inducible Factor 2 Alpha and Potential Roles in Tumor Macrophages: A Review. 低氧诱导因子 2 Alpha 的未充分探索景观及在肿瘤巨噬细胞中的潜在作用：综述。

Oxygen (Basel, Switzerland) Pub Date : 2023-03-01 Epub Date: 2023-01-31 DOI: 10.3390/oxygen3010005

Kayla J Steinberger, Timothy D Eubank

{"title":"The Underexplored Landscape of Hypoxia-Inducible Factor 2 Alpha and Potential Roles in Tumor Macrophages: A Review.","authors":"Kayla J Steinberger, Timothy D Eubank","doi":"10.3390/oxygen3010005","DOIUrl":"10.3390/oxygen3010005","url":null,"abstract":"Low tissue oxygenation, termed hypoxia, is a characteristic of solid tumors with negative consequences. Tumor-associated macrophages (TAMs) accumulate in hypoxic tumor regions and correlate with worse outcomes in cancer patients across several tumor types. Thus, the molecular mechanism in which macrophages respond to low oxygen tension has been increasingly investigated in the last decade. Hypoxia stabilizes a group of hypoxia-inducible transcription factors (HIFs) reported to drive transcriptional programs involved in cell survival, metabolism, and angiogenesis. Though both tumor macrophage HIF-1α and HIF-2α correlate with unfavorable tumor microenvironments, most research focuses on HIF-1α as the master regulator of hypoxia signaling, because HIF-1α expression was originally identified in several cancer types and correlates with worse outcome in cancer patients. The relative contribution of each HIFα subunit to cell phenotypes is poorly understood especially in TAMs. Once thought to have overlapping roles, recent investigation of macrophage HIF-2α has demonstrated a diverse function from HIF-1α. Little work has been published on the differential role of hypoxia-dependent macrophage HIF-2α when compared to HIF-1α in the context of tumor biology. This review highlights cellular HIF-2α functions and emphasizes the gap in research investigating oxygen-dependent functions of tumor macrophage HIF-2α.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":"3 1","pages":"45-76"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9399331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fitting Parameters of a Modified Hill’s Equation and Their Influence on the Shape of the Model Hemoglobin Oxygenation Curve 修正Hill方程的拟合参数及其对模型血红蛋白氧合曲线形状的影响

Oxygen (Basel, Switzerland) Pub Date : 2023-02-28 DOI: 10.3390/oxygen3010007

Igor A. Lavrinenko, G. Vashanov, J. L. Hernández Cáceres, Y. Nechipurenko

{"title":"Fitting Parameters of a Modified Hill’s Equation and Their Influence on the Shape of the Model Hemoglobin Oxygenation Curve","authors":"Igor A. Lavrinenko, G. Vashanov, J. L. Hernández Cáceres, Y. Nechipurenko","doi":"10.3390/oxygen3010007","DOIUrl":"https://doi.org/10.3390/oxygen3010007","url":null,"abstract":"Oxygen binds to hemoglobin cooperatively, and a correct description of this binding is relevant not only for understanding the mechanisms of involved molecular processes but also for clinical purposes. Hill’s equation, derived over a hundred years ago, is still the simplest and most efficient way to perform this description. However, in terms of accuracy, it is inferior to Adair´s equation, which contains more parameters. We proposed to use a modified Hill equation and showed that it allows a more accurate description of the binding of oxygen to hemoglobin than Adair’s equation. At the same time, unlike Adair’s equation, our model retains the physical meaning given to the original Hill equation. We considered and analyzed the influence of the equation parameters on the course of the oxygenation curve and presented the relationship between the fitting parameters and other parameters derived from them in the form of a diagram-graph, which, in our opinion, simplifies the perception of these estimates and can be useful in solving a number of problems for which the traditional way of analyzing the degree of cooperative interaction was via the Hill equation. We suggest that the newly proposed parameter hmax introduced in our model should be regarded as crucial for a better description of the oxygenation curve.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45018528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Early History of Hydrogen and Other Gases in Respiration and Biological Systems: Revisiting Beddoes, Cavallo, and Davy 氢气和其他气体在呼吸和生物系统中的早期历史：重温Beddoes、Cavallo和Davy

Oxygen (Basel, Switzerland) Pub Date : 2023-02-28 DOI: 10.3390/oxygen3010008

J. Hancock, Tyler W. LeBaron

引用次数: 0

Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity 氧电极计时电流法和分光光度法测定过氧化氢酶活性的比较

Oxygen (Basel, Switzerland) Pub Date : 2023-02-03 DOI: 10.3390/oxygen3010006

Michał Szczepańczyk, Lea Paul, T. Ruzgas, Sebastian Björklund

{"title":"Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity","authors":"Michał Szczepańczyk, Lea Paul, T. Ruzgas, Sebastian Björklund","doi":"10.3390/oxygen3010006","DOIUrl":"https://doi.org/10.3390/oxygen3010006","url":null,"abstract":"Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling consistent fallouts. One major problem, however, is that the activity values obtained with different techniques and procedures can vary to a large extent. The aim of this work was to identify experimental conditions that provide similar catalase activity values with two different methods based on either spectrophotometry or chronoamperometry. The investigated parameters include the concentration of catalase and its substrate (H2O2), as well as the effect of deoxygenation of the catalase medium by nitrogen (N2). Within the frame of investigated conditions, we show that spectrophotometry is strongly affected by the catalase concentration, whereas chronoamperometry is shown to be more dependent on the substrate concentration. Deoxygenation leads to elevated catalase activity values in the case of chronoamperometry, whereas it shows no influence on the results obtained with spectrophotometry. In particular, in the case of low substrate concentrations (i.e., low catalase reaction rates), higher and more accurate results are obtained with deoxygenation in the case of chronoamperometry measurements due to minimized oxygen escape. The effect of deoxygenation, giving rise to elevated catalase activity values, however, is not statistically significant at high substrate concentrations, implying that the protocol can be simplified by excluding this step as long as the other parameters are optimized. Finally, by comparing the two methods at different experimental conditions, we identified protocols resulting in similar results, i.e., 10 mM H2O2 and catalase activity of 4–5 U/mL. Based on this work, improved consistency of catalase activity data obtained with different methodologies and in different labs is expected.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45851877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Oxidative Stress in Autism Spectrum Disorder: A Narrative Literature Review 氧化应激在自闭症谱系障碍中的作用：叙事文献综述

Oxygen (Basel, Switzerland) Pub Date : 2023-01-21 DOI: 10.3390/oxygen3010004

Valentina Membrino, Alice Di Paolo, Sonila Alia, Giulio Papiri, A. Vignini

{"title":"The Role of Oxidative Stress in Autism Spectrum Disorder: A Narrative Literature Review","authors":"Valentina Membrino, Alice Di Paolo, Sonila Alia, Giulio Papiri, A. Vignini","doi":"10.3390/oxygen3010004","DOIUrl":"https://doi.org/10.3390/oxygen3010004","url":null,"abstract":"Autism spectrum disorder (ASD) is a multifaceted neurodevelopmental disorder that comprises a complex aetiology, where a genetic component has been suggested, together with multiple environmental risk factors. Because of its increasing incidence in the paediatric population and the lack of successful curative therapies, ASD is one of the most puzzling disorders for medicine. In the last two decades and more, the relationship between oxidative stress (OS) and ASD has been recurrently documented. For this reason, the former hypothesis, according to which reactive oxygen and nitrogen species (ROS and RNS) play an important role in ASD, is now a certainty. Thus, in this review, we will discuss many aspects of the role of OS in ASD. In addition, we will describe, in the context of the most recent literature, the possibility that free radicals promote lipid peroxidation, as well as an increase in other OS biomarkers. Finally, we will outline the possibility of novel nutritional interventions aimed at counteracting ROS production in people with ASD. In fact, new strategies have investigated the possibility that ASD symptoms, as well behavioral anomalies, may be improved after interventions using antioxidants as supplements or included in foods.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43918654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment to the Reviewers of Oxygen in 2022 对2022年《氧气》审稿人的感谢

Oxygen (Basel, Switzerland) Pub Date : 2023-01-18 DOI: 10.3390/oxygen3010003

引用次数: 0

The Pro-Oxidant Effect of Class A CpG ODNs on Human Neutrophils Includes Both Non-Specific Stimulation of ROS Production and Structurally Determined Induction of NO Synthesis A类CpG ODNs对人中性粒细胞的促氧化作用包括对ROS产生的非特异性刺激和结构决定的NO合成诱导

Oxygen (Basel, Switzerland) Pub Date : 2023-01-02 DOI: 10.3390/oxygen3010002

E. Golenkina, S. Galkina, G. Viryasova, G. Sud’ina

引用次数: 0

Effect of Water-Soluble Chlorine-Containing Buckminsterfullerene Derivative on the Metabolism of Reactive Oxygen Species in Human Embryonic Lung Fibroblasts 水溶性氯对人胚肺成纤维细胞活性氧代谢的影响

Oxygen (Basel, Switzerland) Pub Date : 2022-12-27 DOI: 10.3390/oxygen3010001

E. Savinova, T. Salimova, E. Proskurnina, I. V. Rodionov, O. Kraevaya, P. Troshin, L. Kameneva, E. Malinovskaya, O. Dolgikh, N. Veiko, S. Kostyuk

引用次数: 0

Pros and Cons of Two Methods of Anaerobic Alactic Energy Assessment in a High-Intensity CrossFit® Workout 高强度CrossFit®训练中两种厌氧能评估方法的利弊

Oxygen (Basel, Switzerland) Pub Date : 2022-12-17 DOI: 10.3390/oxygen2040042

Manoel Rios, V. Reis, Susana Soares, D. Moreira-Gonçalves, R. Fernandes

{"title":"Pros and Cons of Two Methods of Anaerobic Alactic Energy Assessment in a High-Intensity CrossFit® Workout","authors":"Manoel Rios, V. Reis, Susana Soares, D. Moreira-Gonçalves, R. Fernandes","doi":"10.3390/oxygen2040042","DOIUrl":"https://doi.org/10.3390/oxygen2040042","url":null,"abstract":"The current study aimed to evidence the strengths and weaknesses of two indirect methods for assessing the anaerobic alactic contribution to a specific CrossFit® workout. Thirty experienced crossfitters performed the Fran workout at maximal intensity, and ventilatory data were collected during the recovery period using a telemetric portable gas analyser to assess the oxygen uptake (VO2) of the off-kinetics fast component (Anarecovery). The kinetics of maximal phosphocreatine splitting (AnaPCr) were determined based on the literature. No differences between the two methods were observed (31.4 ± 4.0 vs. 30.4 ± 4.1 kJ for Anarecovery and AnaPCr, respectively). Despite the existence of some caveats (e.g., errors derived from a delay at the onset of VO2 recovery and the assumption of given values in the concentration of phosphocreatine per kilogram of wet muscle, respectively) in both methods, the data indicate that they yield similar results and allow for estimations of alactic energy contribution from a short-duration and high intensity CrossFit® routine. The current data contributes to CrossFit® workout evaluations and training strategies, helping researchers to evaluate crossfitters more accurately. The advantage of the two methods used in the current study is that they are non-invasive, which differs greatly from muscle biopsies.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47873834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Recovery of an Iatrogenic Venous Gaseous Embolism (VGE) Clinical Presentation Thanks to a Timely Hyperbaric Oxygen Therapy (HBOT) Treatment 医源性静脉气体栓塞(VGE)临床表现的完全恢复归功于及时的高压氧治疗(HBOT)

Oxygen (Basel, Switzerland) Pub Date : 2022-12-15 DOI: 10.3390/oxygen2040041

V. Zanon, S. Magri, G. Bonera, E. Camporesi

引用次数: 0