Oxygen (Basel, Switzerland)最新文献_第3页

Mitochondria-Stimulating and Antioxidant Effects of Slovak Propolis Varieties on Bovine Spermatozoa 斯洛伐克蜂胶品种对牛精子线粒体的刺激和抗氧化作用

Oxygen (Basel, Switzerland) Pub Date : 2023-04-26 DOI: 10.3390/oxygen3020013

E. Tvrdá, J. Árvay, M. Ďuračka, M. Kačániová

{"title":"Mitochondria-Stimulating and Antioxidant Effects of Slovak Propolis Varieties on Bovine Spermatozoa","authors":"E. Tvrdá, J. Árvay, M. Ďuračka, M. Kačániová","doi":"10.3390/oxygen3020013","DOIUrl":"https://doi.org/10.3390/oxygen3020013","url":null,"abstract":"Propolis is a natural honeybee product, which is known for its beneficial pharmacological effects and use in traditional medicine. This study aspired to investigate the chemical and antioxidant properties of five propolis specimens, followed by the analysis of their in vitro impact on bovine spermatozoa vitality and oxidative profile. Semen samples from 10 breeding bulls were incubated in the absence or presence of 50 µg/mL propolis extracts, and selected sperm quality parameters including motility, mitochondrial activity, cAMP concentration, NAD+/NADH ratio, reactive oxygen species (ROS) and superoxide production were assessed at 2 h and 24 h. Sperm motion behavior and mitochondrial function were stabilized particularly in the presence of propolis collected from Hrabské and Litpovský Hrádok, Slovakia. At the same time, all propolis specimens acted as significant ROS quenchers and stabilized the oxidative milieu of the sperm cultures. Our data suggest that propolis may stabilize the mitochondrial function of spermatozoa while preventing excessive oxidative insults, thereby showing the potential to sustain the sperm motility of extended semen.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43086141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The On/Off History of Hydrogen in Medicine: Will the Interest Persist This Time Around? 氢在医学上的断断续续的历史：这一次人们的兴趣会持续吗？

Oxygen (Basel, Switzerland) Pub Date : 2023-03-14 DOI: 10.3390/oxygen3010011

Tyler W. LeBaron, K. Ohno, J. Hancock

{"title":"The On/Off History of Hydrogen in Medicine: Will the Interest Persist This Time Around?","authors":"Tyler W. LeBaron, K. Ohno, J. Hancock","doi":"10.3390/oxygen3010011","DOIUrl":"https://doi.org/10.3390/oxygen3010011","url":null,"abstract":"Over 2000 publications including more than 100 human studies seem to indicate that humans have only recently benefited from or known about the medical effects of H2 within the past 15 years. However, we have unknowingly benefited from H2 since the dawn of time, from H2-producing bacteria to the use of naturally occurring hydrogen-rich waters. Moreover, the first writings on the therapeutic effects of H2 date to around 1793. Since then, papers appeared sporadically in the literature every few decades but never exploded until Ohsawa et al. again demonstrated hydrogen’s therapeutic effects in 2007. This landmark paper appears to have been the spark that ignited the medical interest in hydrogen. Although H2 was used in the 1880s to locate intestinal perforations, in the 1940s in deep sea diving, and in the 1960s to measure blood flow, H2 was largely viewed as biologically inert. This review highlights the history of hydrogen in the genesis/evolution of life and its medicinal and non-medicinal use in humans. Although hydrogen medicine has a long and erratic history, perhaps future history will show that, this time around, these 15 years of ignited interest resulted in a self-sustaining explosion of its unique medical effects.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42238764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Acute Red Spinach Extract Ingestion on Repeated Sprint Performance in Division I NCAA Female Soccer Athletes 急性摄入红菠菜提取物对NCAA一级女子足球运动员重复冲刺成绩的影响

Oxygen (Basel, Switzerland) Pub Date : 2023-03-09 DOI: 10.3390/oxygen3010010

Mary V. Raymond, Taylor M. Yount, R. Rogers, C. Ballmann

{"title":"Effects of Acute Red Spinach Extract Ingestion on Repeated Sprint Performance in Division I NCAA Female Soccer Athletes","authors":"Mary V. Raymond, Taylor M. Yount, R. Rogers, C. Ballmann","doi":"10.3390/oxygen3010010","DOIUrl":"https://doi.org/10.3390/oxygen3010010","url":null,"abstract":"Red spinach extract is high in inorganic nitrate/nitrite (NO3/NO2) which has been shown to enhance vascular function, cognition, and physical performance. To date, there have been no investigations as to whether red spinach extract serves as an effective strategy to improve repeated exercise performance, which is applicable to many sports and activities. The purpose of this study was to investigate the effect of acute red spinach extract ingestion on repeated sprint ability in female athletes. Eleven Division I NCAA female athletes (ages 18–24) were recruited. In a double-blinded, randomized, counterbalanced design, participants completed two separate visits each with a different treatment: placebo (placebo; tomato juice) or red spinach extract (~400 mg nitrate). For each trial, participants consumed their respective treatment two hours before exercise. Following a warm-up, participants completed 3 × 15 s Wingate Anaerobic Tests (WAnTs) separated by 2 min of recovery. A capillary blood sample was obtained pre-exercise to measure NO2 concentrations. Performance outcomes, heart rate (heart rate), and rate of perceived exertion were measured following each WAnT. Blood lactate (La-) was obtained prior to exercise (PRE) and after the completion of the repeated sprints (POST). Each visit was separated by a minimal recovery period of 72 h. Mean power (p = 0.204), peak power (p = 0.067), heart rate (p = 0.151), and rate of perceived exertion (p = 0.379) were not significantly different between treatments. POST La- concentration was significantly higher with red spinach extract when compared to the placebo (p = 0.030). Furthermore, the fatigue index (p = 0.018) was significantly lower with red spinach extract. The results do not support the use of red spinach extract for the enhancement of power output during repeated anaerobic exercise. However, it may result in improved La-/H+ removal from the muscle, thereby combating physical fatigue.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49525219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and Enzymatic Analyses to Understand the Accumulation of γ-Aminobutyric Acid in Wheat Grown under Flooding Stress 洪水胁迫下小麦γ-氨基丁酸积累的生化和酶学分析

Oxygen (Basel, Switzerland) Pub Date : 2023-03-01 DOI: 10.3390/oxygen3010009

S. Komatsu, Natsuru Nishiyama, Azzahrah Diniyah

{"title":"Biochemical and Enzymatic Analyses to Understand the Accumulation of γ-Aminobutyric Acid in Wheat Grown under Flooding Stress","authors":"S. Komatsu, Natsuru Nishiyama, Azzahrah Diniyah","doi":"10.3390/oxygen3010009","DOIUrl":"https://doi.org/10.3390/oxygen3010009","url":null,"abstract":"Flooding induces low-oxygen stress, which reduces plant growth. The activity of the γ-aminobutyric acid (GABA) shunt is crucial for plant stress adaptation, in which it acts by changing cytosolic pH, limiting reactive oxygen species production, regulating nitrogen metabolism, and bypassing steps in the tricarboxylic acid cycle. GABA accumulates under osmotic stress as well as flooding stress. To clarify the dynamic roles of GABA accumulation in wheat under flooding stress, biochemical and enzymatic analyses were performed using a plant-derived smoke solution (PDSS), which rescued wheat growth from flooding stress. Alcohol dehydrogenase abundance increased under flooding stress; however, under the same conditions, pyruvic acid content increased only following PDSS application. Glutamic acid content increased under flooding stress, but decreased following the application of PDSS after 2 days of flooding. Glutamate decarboxylase abundance and GABA content increased under flooding stress, and further increased after 1 day of application of PDSS. Succinyl semialdehyde dehydrogenase accumulated after 2 days of flooding. These results suggest that flooding stress increases GABA content along with the increase and decrease of glutamate decarboxylase and succinyl semialdehyde dehydrogenase, respectively. Additionally, PDSS increased GABA content along with the increase of glutamate decarboxylase abundance at the initial stage of application.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46460786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Underexplored Landscape of Hypoxia-Inducible Factor 2 Alpha and Potential Roles in Tumor Macrophages: A Review. 低氧诱导因子 2 Alpha 的未充分探索景观及在肿瘤巨噬细胞中的潜在作用：综述。

Oxygen (Basel, Switzerland) Pub Date : 2023-03-01 Epub Date: 2023-01-31 DOI: 10.3390/oxygen3010005

Kayla J Steinberger, Timothy D Eubank

{"title":"The Underexplored Landscape of Hypoxia-Inducible Factor 2 Alpha and Potential Roles in Tumor Macrophages: A Review.","authors":"Kayla J Steinberger, Timothy D Eubank","doi":"10.3390/oxygen3010005","DOIUrl":"10.3390/oxygen3010005","url":null,"abstract":"Low tissue oxygenation, termed hypoxia, is a characteristic of solid tumors with negative consequences. Tumor-associated macrophages (TAMs) accumulate in hypoxic tumor regions and correlate with worse outcomes in cancer patients across several tumor types. Thus, the molecular mechanism in which macrophages respond to low oxygen tension has been increasingly investigated in the last decade. Hypoxia stabilizes a group of hypoxia-inducible transcription factors (HIFs) reported to drive transcriptional programs involved in cell survival, metabolism, and angiogenesis. Though both tumor macrophage HIF-1α and HIF-2α correlate with unfavorable tumor microenvironments, most research focuses on HIF-1α as the master regulator of hypoxia signaling, because HIF-1α expression was originally identified in several cancer types and correlates with worse outcome in cancer patients. The relative contribution of each HIFα subunit to cell phenotypes is poorly understood especially in TAMs. Once thought to have overlapping roles, recent investigation of macrophage HIF-2α has demonstrated a diverse function from HIF-1α. Little work has been published on the differential role of hypoxia-dependent macrophage HIF-2α when compared to HIF-1α in the context of tumor biology. This review highlights cellular HIF-2α functions and emphasizes the gap in research investigating oxygen-dependent functions of tumor macrophage HIF-2α.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":"3 1","pages":"45-76"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10137047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9399331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fitting Parameters of a Modified Hill’s Equation and Their Influence on the Shape of the Model Hemoglobin Oxygenation Curve 修正Hill方程的拟合参数及其对模型血红蛋白氧合曲线形状的影响

Oxygen (Basel, Switzerland) Pub Date : 2023-02-28 DOI: 10.3390/oxygen3010007

Igor A. Lavrinenko, G. Vashanov, J. L. Hernández Cáceres, Y. Nechipurenko

{"title":"Fitting Parameters of a Modified Hill’s Equation and Their Influence on the Shape of the Model Hemoglobin Oxygenation Curve","authors":"Igor A. Lavrinenko, G. Vashanov, J. L. Hernández Cáceres, Y. Nechipurenko","doi":"10.3390/oxygen3010007","DOIUrl":"https://doi.org/10.3390/oxygen3010007","url":null,"abstract":"Oxygen binds to hemoglobin cooperatively, and a correct description of this binding is relevant not only for understanding the mechanisms of involved molecular processes but also for clinical purposes. Hill’s equation, derived over a hundred years ago, is still the simplest and most efficient way to perform this description. However, in terms of accuracy, it is inferior to Adair´s equation, which contains more parameters. We proposed to use a modified Hill equation and showed that it allows a more accurate description of the binding of oxygen to hemoglobin than Adair’s equation. At the same time, unlike Adair’s equation, our model retains the physical meaning given to the original Hill equation. We considered and analyzed the influence of the equation parameters on the course of the oxygenation curve and presented the relationship between the fitting parameters and other parameters derived from them in the form of a diagram-graph, which, in our opinion, simplifies the perception of these estimates and can be useful in solving a number of problems for which the traditional way of analyzing the degree of cooperative interaction was via the Hill equation. We suggest that the newly proposed parameter hmax introduced in our model should be regarded as crucial for a better description of the oxygenation curve.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45018528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Early History of Hydrogen and Other Gases in Respiration and Biological Systems: Revisiting Beddoes, Cavallo, and Davy 氢气和其他气体在呼吸和生物系统中的早期历史：重温Beddoes、Cavallo和Davy

Oxygen (Basel, Switzerland) Pub Date : 2023-02-28 DOI: 10.3390/oxygen3010008

J. Hancock, Tyler W. LeBaron

引用次数: 0

Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity 氧电极计时电流法和分光光度法测定过氧化氢酶活性的比较

Oxygen (Basel, Switzerland) Pub Date : 2023-02-03 DOI: 10.3390/oxygen3010006

Michał Szczepańczyk, Lea Paul, T. Ruzgas, Sebastian Björklund

{"title":"Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity","authors":"Michał Szczepańczyk, Lea Paul, T. Ruzgas, Sebastian Björklund","doi":"10.3390/oxygen3010006","DOIUrl":"https://doi.org/10.3390/oxygen3010006","url":null,"abstract":"Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling consistent fallouts. One major problem, however, is that the activity values obtained with different techniques and procedures can vary to a large extent. The aim of this work was to identify experimental conditions that provide similar catalase activity values with two different methods based on either spectrophotometry or chronoamperometry. The investigated parameters include the concentration of catalase and its substrate (H2O2), as well as the effect of deoxygenation of the catalase medium by nitrogen (N2). Within the frame of investigated conditions, we show that spectrophotometry is strongly affected by the catalase concentration, whereas chronoamperometry is shown to be more dependent on the substrate concentration. Deoxygenation leads to elevated catalase activity values in the case of chronoamperometry, whereas it shows no influence on the results obtained with spectrophotometry. In particular, in the case of low substrate concentrations (i.e., low catalase reaction rates), higher and more accurate results are obtained with deoxygenation in the case of chronoamperometry measurements due to minimized oxygen escape. The effect of deoxygenation, giving rise to elevated catalase activity values, however, is not statistically significant at high substrate concentrations, implying that the protocol can be simplified by excluding this step as long as the other parameters are optimized. Finally, by comparing the two methods at different experimental conditions, we identified protocols resulting in similar results, i.e., 10 mM H2O2 and catalase activity of 4–5 U/mL. Based on this work, improved consistency of catalase activity data obtained with different methodologies and in different labs is expected.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45851877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Oxidative Stress in Autism Spectrum Disorder: A Narrative Literature Review 氧化应激在自闭症谱系障碍中的作用：叙事文献综述

Oxygen (Basel, Switzerland) Pub Date : 2023-01-21 DOI: 10.3390/oxygen3010004

Valentina Membrino, Alice Di Paolo, Sonila Alia, Giulio Papiri, A. Vignini

{"title":"The Role of Oxidative Stress in Autism Spectrum Disorder: A Narrative Literature Review","authors":"Valentina Membrino, Alice Di Paolo, Sonila Alia, Giulio Papiri, A. Vignini","doi":"10.3390/oxygen3010004","DOIUrl":"https://doi.org/10.3390/oxygen3010004","url":null,"abstract":"Autism spectrum disorder (ASD) is a multifaceted neurodevelopmental disorder that comprises a complex aetiology, where a genetic component has been suggested, together with multiple environmental risk factors. Because of its increasing incidence in the paediatric population and the lack of successful curative therapies, ASD is one of the most puzzling disorders for medicine. In the last two decades and more, the relationship between oxidative stress (OS) and ASD has been recurrently documented. For this reason, the former hypothesis, according to which reactive oxygen and nitrogen species (ROS and RNS) play an important role in ASD, is now a certainty. Thus, in this review, we will discuss many aspects of the role of OS in ASD. In addition, we will describe, in the context of the most recent literature, the possibility that free radicals promote lipid peroxidation, as well as an increase in other OS biomarkers. Finally, we will outline the possibility of novel nutritional interventions aimed at counteracting ROS production in people with ASD. In fact, new strategies have investigated the possibility that ASD symptoms, as well behavioral anomalies, may be improved after interventions using antioxidants as supplements or included in foods.","PeriodicalId":74387,"journal":{"name":"Oxygen (Basel, Switzerland)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43918654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment to the Reviewers of Oxygen in 2022 对2022年《氧气》审稿人的感谢

Oxygen (Basel, Switzerland) Pub Date : 2023-01-18 DOI: 10.3390/oxygen3010003

引用次数: 0