Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献

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Evaluating food web interactions among microcrustaceans and insect in a tropical shallow lake using DNA-based protocol. 使用基于DNA的协议评估热带浅水湖中微型鲸目动物和昆虫之间的食物网相互作用。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-07-01 Epub Date: 2023-11-28
Andrés R Domingos, Abner Carvalho-Batista, Rafael Robles, Marlene S Arcifa, Fernando L Mantelatto
{"title":"Evaluating food web interactions among microcrustaceans and insect in a tropical shallow lake using DNA-based protocol.","authors":"Andrés R Domingos, Abner Carvalho-Batista, Rafael Robles, Marlene S Arcifa, Fernando L Mantelatto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We developed species-specific primers of five microcrustacean preys, <i>Ceriodaphnia richardi</i>, <i>Diaphanosoma</i> cf<i>. brevireme</i>, <i>Daphnia gessneri, Simocephalus serrulatus, Thermocyclops decipiens</i> and <i>Mesocyclops</i> sp., to analyze food-web interactions involving their two insect predators <i>Rheumatobates crassifemur</i> and <i>Martarega uruguayensis</i> distributed in a tropical shallow lake. We designed internal primers of the COI gene (177-282 bp), and tested them, by means of PCR, for specificity and sensitivity. In our tests for specificity, all primers successfully amplified the DNA target but were species-specific failing to amplify the biomarker from any of the other species tested, even in a mixed DNA sample, including predators' DNA. In tests for sensitivity, primers successfully amplified zooplankton biomarkers from low concentration of DNA extractions and also from digestive tract of predators, even after many hours of ingestion. This technique provides a framework as an efficient tool for evaluation of food-web research in natural aquatic environments, where it is impossible to observe if predation occurs. Furthermore, this technique provides an effective solution for the identification of zooplankton species from the predator's digestive tract, where morphological identification alone is sometimes difficult because predators do not consume the prey but feeds using extra-oral digestion, such is the case of heteropterans.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"202-211"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71429942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evolution, systematics, and the unnatural history of mitochondrial DNA. 进化,系统学,以及线粒体DNA的非自然历史。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-05-01 Epub Date: 2021-04-05 DOI: 10.1080/24701394.2021.1899165
Jeffrey H Schwartz
{"title":"Evolution, systematics, and the unnatural history of mitochondrial DNA.","authors":"Jeffrey H Schwartz","doi":"10.1080/24701394.2021.1899165","DOIUrl":"https://doi.org/10.1080/24701394.2021.1899165","url":null,"abstract":"<p><p>The tenets underlying the use of mtDNA in phylogenetic and systematic analyses are strict maternal inheritance, clonality, homoplasmy, and difference due to mutation: that is, there are species-specific mtDNA sequences and phylogenetic reconstruction is a matter of comparing these sequences and inferring closeness of relatedness from the degree of sequence similarity. Yet, how mtDNA behavior became so defined is mysterious. Even though early studies of fertilization demonstrated for most animals that not only the head, but the sperm's tail and mitochondria-bearing midpiece penetrate the egg, the opposite - only the head enters the egg - became fact, and mtDNA conceived as maternally transmitted. When midpiece/tail penetration was realized as true, the conceptions 'strict maternal inheritance', etc., and their application to evolutionary endeavors, did not change. Yet there is mounting evidence of paternal mtDNA transmission, paternal and maternal combination, intracellular recombination, and intra- and intercellular heteroplasmy. Clearly, these phenomena impact the systematic and phylogenetic analysis of mtDNA sequences.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 4","pages":"126-151"},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2021.1899165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25562951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Character-based identification key for commercially important fishes of Pulicat lake: tool for conservation and management. 基于特征的普利卡特湖重要商业鱼类识别密钥:保护与管理工具。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-05-01 Epub Date: 2021-02-10 DOI: 10.1080/24701394.2021.1883009
Rathipriya A, Kathirvelpandian A, Shanmugam S A, Suresh E, Felix N
{"title":"Character-based identification key for commercially important fishes of Pulicat lake: tool for conservation and management.","authors":"Rathipriya A,&nbsp;Kathirvelpandian A,&nbsp;Shanmugam S A,&nbsp;Suresh E,&nbsp;Felix N","doi":"10.1080/24701394.2021.1883009","DOIUrl":"https://doi.org/10.1080/24701394.2021.1883009","url":null,"abstract":"<p><p>Fishes are an important group of vertebrates in the animal world and make a significant contribution to global biodiversity. Fish is used as a source of food and contains many essential vitamins and fatty acids. The study of fish and their stability is important because, from year to year, fish stocks are often very important. For the conservation and management of these dwindling resources, correct identification of species is a prerequisite. Character-based methods of identification are of considerable use in this context, which classify specimens into species using classification rules that compactly describe species in terms of key diagnostic nucleotides in the gene sequences chosen. In this study, a total of 56 species of fishes distributed in Pulicat lake waters is taken as the target group. Mitochondrial CO1 sequences of each species were downloaded and modified. The species-specific diagnostic nucleotides for the selected group of species were identified using the BLOG version 2.0 software. Species-specific probes with a length range of 18-37 bp were designed on the basis of identified diagnostic nucleotide sites. The method is an effective tool for quickly and easily obtaining a significant amount of reliable information and could be used for forensic applications and conservation of fishes in Pulicat Lake.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 4","pages":"120-125"},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2021.1883009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25351547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Genetic population structure of the Blackspot seabream (Pagellus bogaraveo): contribution of mtDNA control region to fisheries management. 黑斑鲷(Pagellus bogaraveo)遗传种群结构:mtDNA控制区对渔业管理的贡献
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-05-01 Epub Date: 2021-02-12 DOI: 10.1080/24701394.2021.1882445
Joana I Robalo, Inês Farias, Sara M Francisco, Karen Avellaneda, Rita Castilho, Ivone Figueiredo
{"title":"Genetic population structure of the Blackspot seabream (<i>Pagellus bogaraveo</i>): contribution of mtDNA control region to fisheries management.","authors":"Joana I Robalo,&nbsp;Inês Farias,&nbsp;Sara M Francisco,&nbsp;Karen Avellaneda,&nbsp;Rita Castilho,&nbsp;Ivone Figueiredo","doi":"10.1080/24701394.2021.1882445","DOIUrl":"https://doi.org/10.1080/24701394.2021.1882445","url":null,"abstract":"<p><p>Marine fisheries management models have traditionally considered biological parameters and geopolitical boundaries. The result is the existence of fisheries management units that do not match genetic populations. However, this panorama is changing with the contribution of genetic and genomic data. <i>Pagellus bogaraveo</i> is a commercially important sparid in the northeast Atlantic, with three stock components being considered by ICES: the Celtic Sea and Bay of Biscay, Atlantic Iberian waters and the Azores. The northern stock collapsed (1975-1985) and is essential to characterize the genetic makeup of the species, particularly in the Iberian Peninsula, where it is managed as a single stock. The mitochondrial control region was used to screen the intraspecific diversity and population structure of individuals from six locations across the species range. The genetic diversity found is similar among sites, and there is differentiation between the Azores and the remaining locations.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 4","pages":"115-119"},"PeriodicalIF":0.0,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2021.1882445","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25360619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Detection of high heteroplasmy in complete loggerhead and hawksbill sea turtles mitochondrial genomes using RNAseq. 利用RNAseq检测红海龟和玳瑁海龟线粒体全基因组的高异质性。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-04-01 Epub Date: 2021-02-25 DOI: 10.1080/24701394.2021.1885389
David Delgado-Cano, Leonardo Mariño-Ramírez, Javier Hernández-Fernández
{"title":"Detection of high heteroplasmy in complete loggerhead and hawksbill sea turtles mitochondrial genomes using RNAseq.","authors":"David Delgado-Cano, Leonardo Mariño-Ramírez, Javier Hernández-Fernández","doi":"10.1080/24701394.2021.1885389","DOIUrl":"10.1080/24701394.2021.1885389","url":null,"abstract":"<p><p>Sea turtle populations around the world face rapid decline due to the effect of anthropogenic and environmental factors. Among the affected populations are those of hawksbill turtles (<i>Eretmochelys imbricata</i>) and loggerhead turtles (<i>Caretta caretta</i>), which is why a greater effort is currently being made in their monitoring and tracing. The intragenic degree of heteroplasmic mutations, commonly associated with diseases of variable symptoms, has not been analyzed in these species. In this study, heteroplasmy in the complete mitogenome (mtDNA) of three loggerhead turtles and one hawksbill turtle was identified from data obtained by RNAseq. Individuals Cc3, Ei1, Cc1 and Cc2 presented 0.3, 1.7, 1.8 and 7.1% of heteroplasmic mutations in all their mtDNA, respectively. The protein-coding genes that presented the highest percentage of heteroplasmy were <i>ND4</i> and <i>ND5</i> in individual Cc2 with 16 and 38.6%, respectively. Of the tRNA genes, only tRNA<sup>Tyr</sup> was heteroplasmic in the four individuals with 5.63% (Cc1), 25.35% (Ei1 and Cc2) and 49.3% (Cc3). In this study, we identified the critical sites of heteroplasmy in each individual and the genetic variability of their mitogenomes. The data obtained represents the baseline for future projects that evaluate the population status of these species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 3","pages":"106-114"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179041/pdf/nihms-1706379.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25404330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryptic species diversity and molecular diagnosis of Channa orientalis; an endemic freshwater fish of Sri Lanka. 中国侧柏的隐种多样性及分子诊断斯里兰卡特有的淡水鱼。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-04-01 Epub Date: 2021-01-27 DOI: 10.1080/24701394.2021.1876040
Hansani Ekanayake, Naalin Perera, Kanishka D Ukuwela, Chaminda N Walpita, Suranga P Kodithuwakku, Sandun J Perera
{"title":"Cryptic species diversity and molecular diagnosis of <i>Channa orientalis</i>; an endemic freshwater fish of Sri Lanka.","authors":"Hansani Ekanayake,&nbsp;Naalin Perera,&nbsp;Kanishka D Ukuwela,&nbsp;Chaminda N Walpita,&nbsp;Suranga P Kodithuwakku,&nbsp;Sandun J Perera","doi":"10.1080/24701394.2021.1876040","DOIUrl":"https://doi.org/10.1080/24701394.2021.1876040","url":null,"abstract":"<p><p>Fish genetic resources and diversity are very important aspects of environmental management and fisheries and are vital for making decisions on their commercial exploitation as well as conservation. The snakehead fishes in the world have significant economic importance as food and ornamental fish. A clear understanding of species' taxonomic status and genetic diversity is important for the utilization and implementation of conservation and management practices. <i>Channa orientalis</i> is a snakehead endemic to Sri Lanka that is heavily utilized in the ornamental fish export trade. Its genetic diversity has not yet been fully understood and it is difficult to distinguish it from closely resembling species. Therefore, we examined the genetic diversity of <i>C. orientalis</i> and developed a DNA-based marker that permits accurate, low cost, and reliable identification of <i>C. orientalis</i>. Determination of genetic diversity was mainly carried out through genetic analysis of the mitochondrial <i>cytochrome c oxidase subunit 1</i> (<i>MT-CO1</i>) gene. The development of the DNA-based marker for the identification of <i>C. orientalis</i> was done through Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. Our analyses confirmed the presence of two distinct genetically divergent and geographically separated lineages of <i>C. orientalis</i> in Sri Lanka. The fast cost-effective gel-based PCR-RFLP marker method developed by us was successful in diagnosing <i>C. orientalis</i> from its closely resembling species. Thus, we believe our findings on the cryptic diversity and diagnostic methods will have important implications for the conservation and management of this endemic species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 3","pages":"77-84"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2021.1876040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38865588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Higher-level phylogenetic relationships of rove beetles (Coleoptera, Staphylinidae) inferred from mitochondrial genome sequences. 从线粒体基因组序列推断花叶甲虫(鞘翅目,葡萄球菌科)的高级系统发育关系。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-04-01 Epub Date: 2021-02-11 DOI: 10.1080/24701394.2021.1882444
Nan Song, Qing Zhai, Yaling Zhang
{"title":"Higher-level phylogenetic relationships of rove beetles (Coleoptera, Staphylinidae) inferred from mitochondrial genome sequences.","authors":"Nan Song,&nbsp;Qing Zhai,&nbsp;Yaling Zhang","doi":"10.1080/24701394.2021.1882444","DOIUrl":"https://doi.org/10.1080/24701394.2021.1882444","url":null,"abstract":"<p><p>Rove beetles (Staphylinidae) and allied families constitute a huge radiation of Coleoptera, but basal relationships in this group remain controversial. In this study, we newly sequenced eight mitogenomes of representatives of Staphylinidae by using next-generation sequencing method. Together with 99 existing mitogenomes of Staphyliniformia, (sub)family relationships were investigated with ML and Bayesian searches under various substitution models and data recoding schemes. The results consistently supported Scydmaenidae and Silphidae to be subordinate groups of Staphylinidae. Within the monophyletic Staphylinidae (including Scydmaenidae and Silphidae), the hypothesis of four major subfamily groups cannot be confirmed. Bayesian inferences under the site-heterogeneous mixture model generally supported the basal position of major clades corresponding to the Omaliine group. At the subfamily level, the monophyly of Pselaphinae, Oxytelinae, Scaphidiinae, Steninae and Staphylininae was supported. However, the subfamilies Omaliinae, Tachyporinae, Aleocharinae and Paederinae were each non-monophyletic.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 3","pages":"98-105"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2021.1882444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25356066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Phylogeography and demographic expansion in the widely distributed horned passalus beetle, Odontotaenius disjunctus (coleoptera: Passalidae). 分布广泛的有角passalus, Odontotaenius disjunctus的系统地理学和人口统计学扩展(鞘翅目:passalides科)。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-04-01 Epub Date: 2021-02-16 DOI: 10.1080/24701394.2021.1882443
Megan Whitaker, Taylor Procter, Frank M Fontanella
{"title":"Phylogeography and demographic expansion in the widely distributed horned passalus beetle, <i>Odontotaenius disjunctus</i> (coleoptera: Passalidae).","authors":"Megan Whitaker,&nbsp;Taylor Procter,&nbsp;Frank M Fontanella","doi":"10.1080/24701394.2021.1882443","DOIUrl":"https://doi.org/10.1080/24701394.2021.1882443","url":null,"abstract":"<p><p>Dynamic climatic oscillations during the Pleistocene had profound effects on the distribution of species across North America. Although the role of historical climate change on speciation remains controversial, the impact on genetic variation within species has been well documented. Analyses of mtDNA sequences from the <i>cytochrome oxidase I</i> gene (911 bp) for 115 individuals of <i>Odontotaenius disjunctus</i> was combined with ecological niche modelling (ENM) to infer the demographic and population differentiation scenarios under present and past conditions. We inferred three lineages that diverged during the Pleistocene and replace each other geographically across the eastern United States. One of these lineages traverses previously identified genetic barriers for terrestrial animals including the Mississippi and Apalachicola Rivers and the Appalachian Mountains. We observed overlapping ranges between two haplotype groups as well as a region of secondary contact associated with ecological transition zone in northern Florida. The two continental lineages depict a genetic signature of a recent population increase associated with expanding niche envelope, whereas the clade restricted to peninsular Florida shows stable populations in a shrinking niche envelope. Given the lack of ecological separation, overlapping distribution of haplogroups and the presence of secondary contact zones, the taxonomic status of these lineages must await robust testing using multilocus DNA data to assess species boundaries.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 3","pages":"85-97"},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2021.1882443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25373552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Mitochondrial ND1 T4216C and ND2 C5178A mutations are associated with maternally transmitted diabetes mellitus. 线粒体ND1 T4216C和ND2 C5178A突变与母体遗传的糖尿病有关。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-03-01 Epub Date: 2020-12-07 DOI: 10.1080/24701394.2020.1856101
Zhaochang Jiang, Lili Teng, Shunrong Zhang, Yu Ding
{"title":"Mitochondrial <i>ND1</i> T4216C and <i>ND2</i> C5178A mutations are associated with maternally transmitted diabetes mellitus.","authors":"Zhaochang Jiang,&nbsp;Lili Teng,&nbsp;Shunrong Zhang,&nbsp;Yu Ding","doi":"10.1080/24701394.2020.1856101","DOIUrl":"https://doi.org/10.1080/24701394.2020.1856101","url":null,"abstract":"<p><p>Mutations in mitochondrial DNA (mtDNA) are important causes for type 2 diabetes mellitus (T2DM). To investigate the association between mtDNA mutations/variants and diabetes, we reported here clinical, genetic and biochemical characterization of a Chinese pedigree with maternally transmitted T2DM. Using PCR and direct sequencing analysis of mitochondrial genomes from the matrilineal relatives, we identified two potential pathogenic mutations, m.T4216C (p.Y304H) and m.C5178A (p.L237M) in the <i>ND1</i> and <i>ND2</i> genes, respectively, together with a set of genetic polymorphisms belonging to the human mitochondrial haplogroup D4b. Moreover, by isolating and analyzing polymononuclear leukocytes generated from the T2DM patients and controls, we identified lower levels of mitochondrial membrane potential and ATP production in T2DM patients than in the controls, in contrast, a significantly higher level of reactive oxygen species was observed in the T2DM patients carrying both of the m.T4216C and m.C5178A mutations (<i>p</i> < 0.05 for all). In addition, the plasma levels of malondialdehyde and 8-hydroxydeoxyguanosine in the T2DM patients markedly increased, while the level of superoxide dismutase decreased (<i>p</i> < 0.05 for all). Taken together, our data indicated that the <i>ND1</i> T4216C and <i>ND2</i> C5178A mutations may lead to oxidative stress and impair the mitochondrial function, and this, in turn, might have been involved in the pathogenesis and progression of T2DM in this pedigree. Thus, our study provides novel insight into the pathophysiology of T2DM that is manifested by mitochondrial dysfunction.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 2","pages":"59-65"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1856101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38680148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Maternal inheritance of mitochondrial DNA in mice after inter-species hybridization and 138 generations of backcrossing. 种间杂交和回交138代后小鼠线粒体DNA的母系遗传。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-03-01 Epub Date: 2021-01-08 DOI: 10.1080/24701394.2020.1865940
Dan Wharton, Kevin C Morey, Robert Hanner
{"title":"Maternal inheritance of mitochondrial DNA in mice after inter-species hybridization and 138 generations of backcrossing.","authors":"Dan Wharton,&nbsp;Kevin C Morey,&nbsp;Robert Hanner","doi":"10.1080/24701394.2020.1865940","DOIUrl":"https://doi.org/10.1080/24701394.2020.1865940","url":null,"abstract":"This study represents a continuation of a backcrossing experiment initiated in 1982 and reported on earlier after seven generations of backcrossing (Gyllensten et al. 1985) and again after 26 generations (Gyllensten et al. 1991). For the better part of 50 years, it has been demonstrated that mitochondrial DNA (mtDNA) has a largely, if not exclusively maternal mode of inheritance among the multicellular organisms studied to date (Dawid and Blackler 1972; Hutchison et al. 1974; Francisco et al. 1979; Giles et al. 1980; Reilly and Thomas 1980 as cited in Gyllensten et al. 1985). Few studies of invertebrates and no studies of vertebrates have examined maternal inheritance of mtDNA over the course of 139 generations as is the case here (Degrugillier and Newman 1993). Our initial results identify 100% conformity of mtDNA with M. spretus suggesting that any potential paternal contribution of mtDNA to this mouse lineage will require more detailed analysis to detect it.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 2","pages":"73-75"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1865940","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38795878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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