Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献_第6页

Expression of concern. 表达关心。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-03-01 Epub Date: 2021-01-11 DOI: 10.1080/24701394.2020.1857198

引用次数: 0

Morphological and molecular analysis of the freshwater copepod Heliodiaptomus viduus (Calanoida: Diaptomidae). 淡水桡足类双足鱼的形态与分子分析(鱼纲:双足鱼科)。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-03-01 Epub Date: 2020-11-12 DOI: 10.1080/24701394.2020.1845324

Mayavan Karthika, Shameem Shabana, Venkatachalam Ramasubramanian

{"title":"Morphological and molecular analysis of the freshwater copepod Heliodiaptomus viduus (Calanoida: Diaptomidae).","authors":"Mayavan Karthika, Shameem Shabana, Venkatachalam Ramasubramanian","doi":"10.1080/24701394.2020.1845324","DOIUrl":"https://doi.org/10.1080/24701394.2020.1845324","url":null,"abstract":"Morphological identification of the commonest Indian freshwater calanoid copepod Heliodiaptomus viduus has been facing a lot of controversies. To solve this problem, additional molecular support is needed. Hence, molecular analysis, along with morphological identification of the species using scanning electron microscopy (SEM) and optical microscopy, was carried out. The genetic distance between the intraspecific sequences in the 18SrRNA observed to be 0.000, while the interspecific distance within genus sequences varied from 0.000 to 0.002. Besides, it was noted that both the sequences (intraspecific) were present within a single clade of the phylogenetic trees with 100% bootstrap value. The genetic distance between the intraspecific sequences in the mtCOI observed to be 0.000, while the interspecific distance within genus sequences varied from 0.000 to 0.267 and lay in a separate clade in the phylogenetic tree from the within genus clade. Besides, it was noted that both the sequences (intraspecific) were present within a single clade of the phylogenetic tree with above 75% bootstrap value. Hence, the 18S rRNA (MH145356, MK457456) and mtCOI (MK621900) gene sequences of the present study are well supported the identification of H. viduus and it serves as the first molecular database besides providing morphological authentication (SEM images) of H.viduus. This combined analysis is envisioned to be helpful in selecting the ecologically and nutritionally important species for practical aquaculture.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 2","pages":"43-58"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1845324","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38594351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeographic analyses of poplar revealed potential glacial refugia and allopatric divergence in southwest China. 杨树的系统地理学分析揭示了西南地区潜在的冰川避难所和异域分化。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-03-01 Epub Date: 2020-12-11 DOI: 10.1080/24701394.2020.1856828

Ang Li, Zhe Hou

{"title":"Phylogeographic analyses of poplar revealed potential glacial refugia and allopatric divergence in southwest China.","authors":"Ang Li, Zhe Hou","doi":"10.1080/24701394.2020.1856828","DOIUrl":"https://doi.org/10.1080/24701394.2020.1856828","url":null,"abstract":"The distribution pattern and genetic structure of plant species have been profoundly influenced by climate oscillations. Phylogeographic analyses have been numerously conducted in biodiversity hotspot regions and some general conclusions have been documented. However, other regions have received less attentions and these places may serve as potential glacial refugia for plant species to survive the Quaternary glaciation. Here, we used six nuclear and three cpDNA markers to estimate the phylogeographic pattern of Populus davidiana, a widespread species distributed in southwest China. As a widely distributed species in southwest China, the nucleotide diversity of P. davidiana was relatively high (Na = 6.28, HO = 0.534, and HE = 0.658). Genetic differentiation (FST) between the two main distribution regions, Yunnan and Guizhou provinces, was 0.21221. According to the composition of chloroplast haplotypes and the result of structure in these populations, we clearly distinguished two distantly sublineages corresponding to two distribution regions. Results of the Mantel test showed that there was a significant correlation between genetic distance and geographical distance (R2 = 0.8252, p<.05). The topographically heterogeneous regions and the low dispersal ability of seed and pollen may lead to high genetic differentiation between these two regions. A potential glacial refugia for P. davidiana located in adjacent regions to the Hengduan range was revealed and allopatric divergence in separated glacial refugia may directly lead to the present phylogeographic pattern of this species.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 2","pages":"66-72"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1856828","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38698890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic diversity of Rhinogobius delicatus (Perciformes: Gobiidae): origins of the freshwater fish in East Taiwan. 台湾东部淡水鱼的遗传多样性(鳍形目:虾蛄科)

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-01-01 Epub Date: 2020-11-10 DOI: 10.1080/24701394.2020.1844678

Yu-Min Ju, Jui-Hsien Wu, Kui-Ching Hsu, Yu-Wen Chiu, Wei-Kuang Wang, Chih-Wei Chen, Hung-Du Lin

{"title":"Genetic diversity of Rhinogobius delicatus (Perciformes: Gobiidae): origins of the freshwater fish in East Taiwan.","authors":"Yu-Min Ju, Jui-Hsien Wu, Kui-Ching Hsu, Yu-Wen Chiu, Wei-Kuang Wang, Chih-Wei Chen, Hung-Du Lin","doi":"10.1080/24701394.2020.1844678","DOIUrl":"https://doi.org/10.1080/24701394.2020.1844678","url":null,"abstract":"Mitochondrial DNA cytochrome b and d-loop sequences (1,984 bp) from 92 specimens of the freshwater goby Rhinogobius delicatus from seven drainages in East Taiwan were identified as two major lineages exhibiting a southern or northern distribution. The existence of low genetic diversity, a pattern of population decline and high population differentiation (FST=0.711) support the need for the development of management strategies for the conservation of localized populations. The results of a statistical dispersal-vicariance analysis suggested that the ancestral populations of R. delicatus were widely distributed in East Taiwan. Compared with the phylogeographic patterns of the other endemic eastern Taiwan freshwater fishes, Onychostoma alticorpus, Aphyocypris kikuckii and Hemimyzon taitungensis, our study suggests that the freshwater fishes colonized East Taiwan through northeastern and southwestern Taiwan, although the ancestral populations colonized the island before it reached its present shape.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 1","pages":"12-19"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1844678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38592601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Phylogeography, genetic diversity and population structure of the freshwater stingray, Paratrygon aiereba (Müller & Henle, 1841) (Myliobatiformes: Potamotrygonidae) in the Colombian Amazon and Orinoco basins. 哥伦比亚亚马逊河和奥里诺科河流域淡水黄貂鱼Paratrygon aiereba (m<s:1> ller & Henle, 1841) (myliobatiforma: potamotygonidae)的系统地理学、遗传多样性和种群结构。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-01-01 Epub Date: 2020-12-21 DOI: 10.1080/24701394.2020.1844679

Maira A Rizo-Fuentes, Camilo A Correa-Cárdenas, Carlos A Lasso, Mónica A Morales-Betancourt, Dalia C Barragán-Barrera, Susana Caballero

{"title":"Phylogeography, genetic diversity and population structure of the freshwater stingray, Paratrygon aiereba (Müller & Henle, 1841) (Myliobatiformes: Potamotrygonidae) in the Colombian Amazon and Orinoco basins.","authors":"Maira A Rizo-Fuentes, Camilo A Correa-Cárdenas, Carlos A Lasso, Mónica A Morales-Betancourt, Dalia C Barragán-Barrera, Susana Caballero","doi":"10.1080/24701394.2020.1844679","DOIUrl":"https://doi.org/10.1080/24701394.2020.1844679","url":null,"abstract":"The freshwater stingray Paratrygon aiereba have coloration, osteological and morphometric variations that could suggest the existence of more than one species in Colombia. In order to evaluate the phylogeography, population structure and genetic diversity for P. aiereba distributed in the Amazon and Orinoco basins, we amplified Cytochrome oxidase subunit 1 (COI) partial region of mitochondrial DNA (mtDNA) in 50 samples from eight different sub-basins. Our results suggest three phylogroups and a vicariance event occurred 43 million years ago proposing how Paratrygon diverged into the basins. A high population structure (ΦST = 0.692; p < 0.005) and a value of (K) of 3 were defined. A high genetic diversity within phylogroups was found: Phylogroup A (h = 0.64; π% = 2.48), Phylogroup B (h = 0.552; π% = 1.67), and Phylogroup C (h = 0.49; π% = 0.73). These results should be considered in local management plans, conservation programs and reclassification in at least Amazon and Orinoco.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 1","pages":"20-33"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1844679","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38396493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Mitochondrial heteroplasmy and pseudogenes in the freshwater prawn, Macrobrachium amazonicum (Heller, 1862): DNA barcoding and phylogeographic implications. 亚马逊沼虾线粒体异质性和假基因(Heller, 1862): DNA条形码及其系统地理意义。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-01-01 Epub Date: 2020-11-09 DOI: 10.1080/24701394.2020.1844677

Gabriel Iketani, Luciana Pimentel, Ezequias Dos Santos Torres, Péricles Sena do Rêgo, Iracilda Sampaio

{"title":"Mitochondrial heteroplasmy and pseudogenes in the freshwater prawn, Macrobrachium amazonicum (Heller, 1862): DNA barcoding and phylogeographic implications.","authors":"Gabriel Iketani, Luciana Pimentel, Ezequias Dos Santos Torres, Péricles Sena do Rêgo, Iracilda Sampaio","doi":"10.1080/24701394.2020.1844677","DOIUrl":"https://doi.org/10.1080/24701394.2020.1844677","url":null,"abstract":"The mitochondrial cytochrome oxidase c subunit 1 (COI) gene has been widely used in phylogenetic studies of crustaceans and analyses in population genetics. As COI studies have become more popular, there has been an increase in the number of reports of the presence of nuclear insertions of mitochondrial DNA (Numts) and mitochondrial heteroplasmy. Here, we provide evidence of both types of event in the COI sequences of Macrobrachium amazonicum, an economically important freshwater prawn, which is widespread in South America. Heteroplasmy and Numts were confirmed by different methods of DNA extraction (genomic, mitochondrial, and nuclear-enriched DNA), cloning, and sequencing, and were observed in 11 of the 14 populations sampled, primarily in the Amazon region. We discuss how the occurrence of these events affects the interpretation of the genetic relationships among the M. amazonicum populations, and we recommend caution when using COI for genetic inferences in prawns of the genus Macrobrachium, and in particular that any analysis should include nuclear markers.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1844677","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38579331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Analyses of mitogenomic markers shed light on the divergence, population dynamics, and demographic history of Pakistani chickens. 有丝分裂基因组标记分析揭示了巴基斯坦鸡的分化、种群动态和人口统计学历史。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2021-01-01 Epub Date: 2020-11-12 DOI: 10.1080/24701394.2020.1845323

Sawar Khan, Ayesha Nisar, Habib Ahmad, Sardar Azhar Mehmood, Muddassar Hameed, Xiaochao Zhao, Xiangshu Yang, Xingang Feng

{"title":"Analyses of mitogenomic markers shed light on the divergence, population dynamics, and demographic history of Pakistani chickens.","authors":"Sawar Khan, Ayesha Nisar, Habib Ahmad, Sardar Azhar Mehmood, Muddassar Hameed, Xiaochao Zhao, Xiangshu Yang, Xingang Feng","doi":"10.1080/24701394.2020.1845323","DOIUrl":"https://doi.org/10.1080/24701394.2020.1845323","url":null,"abstract":"Pakistan is one of a few sites, associated with the earliest known independent domestication event in the evolutionary history of chicken, which is socio-economically and historically the most important poultry bird in the country. However, the divergence, past population dynamics, and demographic history of Pakistani chickens have not been addressed so far. Therefore, we herein investigated the indigenous Pakistani chickens using mitogenomic markers. We first prepared individual DNA samples from the chicken feathers, and generated nucleotide sequence data, which was then subjected to various population genetics analyses. In molecular phylogenetic analysis, the Pakistani chickens were clustered under nine different clades. Among the wild fowls, the Indian red jungle fowl (IRJF) shared very close affinities to Pakistani chickens. The Bayesian skyline plot showed an increase in the effective population size of Pakistani chickens during the last 50 years. Finally, a time-calibrated phylogeny inferred molecular divergence of the Pakistani chickens. A molecular rate of 3.6 × 10-6 mutations/site/year (95% HPD interval: 2.28 × 10-8 to 9.32 × 10-6) was estimated for the data set. In a rooted tree with root-age of 12058 years (95% HPD interval: 1161-38411), the Pakistani chicken haplotypes showed divergence from IRJF haplotypes around 6987 years (95% HPD interval: 1132-20746) ago, and they shared their most recent common ancestor with Gallus gallus spadiceus, and G. g. jabouillei at the root of the tree. Overall, these results suggest that Pakistani chicken haplotypes share their ancestral gene pool with the IRJF as compared to other red jungle fowl subspecies.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"32 1","pages":"34-42"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1845323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38592273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards understanding the evolutionary dynamics of mtDNA. 了解mtDNA的进化动力学。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2020-12-01 Epub Date: 2020-10-07 DOI: 10.1080/24701394.2020.1830076

Samuel G Towarnicki, J William O Ballard

{"title":"Towards understanding the evolutionary dynamics of mtDNA.","authors":"Samuel G Towarnicki, J William O Ballard","doi":"10.1080/24701394.2020.1830076","DOIUrl":"https://doi.org/10.1080/24701394.2020.1830076","url":null,"abstract":"Historically, mtDNA was considered a selectively neutral marker that was useful for estimating the population genetic history of the maternal lineage. Over time there has been an increasing appreciation of mtDNA and mitochondria in maintaining cellular and organismal health. Beyond energy production, mtDNA and mitochondria have critical cellular roles in signalling. Here we briefly review the structure of mtDNA and the role of the mitochondrion in energy production. We then discuss the predictions that can be obtained from quaternary structure modelling and focus on mitochondrial complex I. Complex I is the primary entry point for electrons into the electron transport system is the largest respiratory complex of the chain and produces about 40% of the proton flux used to synthesize ATP. A focus of the review is Drosophila's utility as a model organism to study the selective advantage of specific mutations. However, we note that the incorporation of insights from a multitude of systems is necessary to fully understand the range of roles that mtDNA has in organismal fitness. We speculate that dietary changes can illicit stress responses that influence the selective advantage of specific mtDNA mutations and cause spatial and temporal fluctuations in the frequencies of mutations. We conclude that developing our understanding of the roles mtDNA has in determining organismal fitness will enable increased evolutionary insight and propose we can no longer assume it is evolving as a strictly neutral marker without testing this hypothesis.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 8","pages":"355-364"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1830076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38464056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitochondrial DNA diversity and population structure of Laodelphax striatellus across a broad geographic area in China. 中国大地理区域纹状斑蝽线粒体DNA多样性及种群结构。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2020-12-01 Epub Date: 2020-10-08 DOI: 10.1080/24701394.2020.1830075

Lei Han, Jing-Tong Zhang, Ming-Ming Wang, Ke-Xin Zhu, Xing-Ya Wang

{"title":"Mitochondrial DNA diversity and population structure of Laodelphax striatellus across a broad geographic area in China.","authors":"Lei Han, Jing-Tong Zhang, Ming-Ming Wang, Ke-Xin Zhu, Xing-Ya Wang","doi":"10.1080/24701394.2020.1830075","DOIUrl":"https://doi.org/10.1080/24701394.2020.1830075","url":null,"abstract":"The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Hemiptera: Delphacidae), is a crucial devastating rice pest in East Asia. To effectively control this pest, we investigate the genetic diversity, genetic differentiation and genetic structure of 49 populations in China based on a 596 bp fragment of the mitochondrial DNA cytochrome c oxidase subunit I (mtDNA COI) gene. Overall, 83 haplotypes were detected in 1253 mtDNA COI sequences. High levels of genetic variability (Hd = 0.756 ± 0.009, π = 0.00416 ± 0.00011) and genetic differentiation (FST = 0.262, p < .001) were observed. Bayesian inference phylogenetic and median-joining haplotype network analyses indicated no obvious geographical distribution pattern among haplotypes. Hierarchical AMOVA and SAMOVA revealed no genetically distinct groups and lack of obvious phylogeographic structure. Isolation by distance (IBD) analysis results demonstrated no correlation between genetic differentiation and geographic distance. Finally, the demographic history of SBPH examined by neutrality tests and mismatch distribution analyses illustrated a sudden population expansion at the large spatial scale in China.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 8","pages":"346-354"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1830075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38465964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Morphology and molecular identification of the zoological origin of medicinal seahorses in Chinese herbal markets. 中草药市场药用海马的形态及动物来源的分子鉴定。

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2020-12-01 Epub Date: 2020-09-08 DOI: 10.1080/24701394.2020.1815719

Xi Wang, Hongjin Zhong, Jinlin Guo, Feixia Hou

{"title":"Morphology and molecular identification of the zoological origin of medicinal seahorses in Chinese herbal markets.","authors":"Xi Wang, Hongjin Zhong, Jinlin Guo, Feixia Hou","doi":"10.1080/24701394.2020.1815719","DOIUrl":"https://doi.org/10.1080/24701394.2020.1815719","url":null,"abstract":"Seahorses are a charismatic group of fish that have high economic value for their unique appearance and important medicinal values. They were heavily traded as traditional Chinese medicines. Authenticating the zoological origin of medicinal seahorses is very difficult because of their similar morphology. To study the identification characteristics of dried seahorse, and to provide a scientific basis for seahorse resource conservation and market supervision, 64 dried specimens from China's four major pharmaceutical markets were investigated based on morphology and COI sequences. Sixty-four COI sequences of 662 bp length revealed 43 unique haplotypes, which were divided into 12 main clades in both NJ and UPGMA phylogenetic trees. Eleven species including Hippocampus spinosissimus, H. barbouri, H. kuda, H. comes, H. histrix, H. trimaculatus, H. kelloggi, H. ingens, H. mohnikei, H. erectus and H. jayakari were clustered on different branches and showed respective monophyly. The results were confirmed by morphology and BLAST analysis. Hippocampus capensis and H. fuscus, which were clustered together in the phylogenetic tree, could be distinguished by different morphology. The morphological and molecular determination revealed 13 seahorse species in Chinese herbal markets. The method of DNA sequences analysis combined with morphological characteristics is conducive to accurately identify the zoological origin of commercial seahorses.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 8","pages":"335-345"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1815719","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38352306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8