Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献

{"title":"Comparative phylogeny of (<i>Schizothorax esocinus</i>) with reference to 12s and 16 sribosomal RNA from River Swat, Pakistan.","authors":"Aqsa Rehman,&nbsp;Muhammad Fiaz Khan,&nbsp;Saira Bibi,&nbsp;Faisal Nouroz","doi":"10.1080/24701394.2020.1741561","DOIUrl":"https://doi.org/10.1080/24701394.2020.1741561","url":null,"abstract":"<p><p><i>Schizothorax esocinus</i> is one of the important cold water delicious fish belonging to family Cyprinidae and found in River Swat which is one of the main river flowing in Khyber Pakhtunkhwa, Pakistan.The complete mitochondrial genomes of the <i>S. esocinus</i> species was found to be 16,591bp. Phylogenetic analysis of <i>S. esocinus</i> based on 12 s ribosomal RNA and 16S rRNA confirmed that, the phylogenetic position of <i>S. esocinus</i> was slightly different, but clustered closed to <i>S. plagiostomus, S. labiatus</i>, <i>S. richardsonii, S. nepalensis,</i> and <i>S. progastus</i> predicting a close homology in genome and hence a monophyletic line of evolution also this specie showed close relationship and homologs to the <i>Aspiorhynchus laticeps, Spinibarbus sinensis, Aspiorhynchus laticeps,</i> and <i>Percocypris</i> respectively. This study provided phylogenetic relationship between Schizothoracinae fishes and with the other fishes of family cyprinidae even at the subfamily level.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 2","pages":"81-85"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1741561","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37754306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic differentiation and phylogenetic relationship of 11 Asian Sisorinae genera (Siluriformes: Sisoridae) with new record of <i>Pseudolaguvia foveolata</i>.","authors":"Kavita Kumari,&nbsp;Manas H M,&nbsp;Archana Sinha,&nbsp;Satish Kumar Koushlesh,&nbsp;Soma Das Sarkar,&nbsp;Simanku Borah,&nbsp;Raju BaItha,&nbsp;Bijay Kumar Behera,&nbsp;Basanta Kumar Das","doi":"10.1080/24701394.2020.1714605","DOIUrl":"https://doi.org/10.1080/24701394.2020.1714605","url":null,"abstract":"<p><p>Studies on <i>Sisorinae</i> systematics have been largely restricted to morphological data with few studies on examination of phylogenetic relations. However, no study has been done to evaluate genetic distance of the genera under <i>Sisorinae</i> sub-family and detailed phylogenetic relations within it. We used nuclear recombination activating 2 (<i>rag2</i>) gene and mitochondrial cytochrome c oxidase I (<i>COI</i>) gene from 64 species to examine genetic differentiation and phylogenetic relationships within 11 Asian <i>Sisorinae</i> genera. The range of interspecies K2P distance for <i>rag2</i> was 0-0.061 and <i>COI</i> was 0-0.204. Phylogenetic analysis based on maximum likelihood (ML) and Bayesian (BI) approaches for each locus individually and for the concatenated <i>rag2</i> and <i>COI</i> sequences revealed three major subclades viz. Bagariini, Sisorini and Erethistini under subfamily Sisorinae. The analysis based on <i>COI</i> gene showed ((Sisorini, Bagariini), Erethistini) relationship. <i>Rag</i>2 and combined <i>rag2</i> and <i>COI</i> showed ((Sisorini, Erethistini), Bagariini) relationship. Combined <i>rag</i>2 and <i>COI</i> analyses resulted into better resolved trees with a good bootstrap support. In this study, new record of <i>Pseudolaguvia foveolata</i> (Erethistini) has been documented based on 13 specimens collected from Torsa River, Jaldapara, Alipurduar district, West Bengal, India (26°43'44.66″ N and 89°19'32.34″ E), extending its distribution range in Brahmaputra drainage, India. The genetic distance between the <i>P. foveolata</i> new record and the reported <i>P. foveolata</i> (holotype: UMMZ 244867) was 0.00 at both <i>rag2</i> and <i>COI</i> locus and it was further grouped with <i>P. foveolata</i> Type specimen (holotype: UMMZ 244867) with 100% bootstrap support. This report gives additional information on occurrence of the species <i>P. foveolata</i>, along with discussion on morphometric, meristic and molecular (<i>COI</i> and <i>rag2</i> gene) data.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 1","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2020.1714605","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37562368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to the critique of Sameer Padhye and Neelesh Dahanukar (2019).","authors":"Shil Abhyankar,&nbsp;Kshama Khobragade,&nbsp;Ganesh Khanwelkar,&nbsp;Anita Tiknaik,&nbsp;Gulab Khedkar","doi":"10.1080/24701394.2019.1703964","DOIUrl":"https://doi.org/10.1080/24701394.2019.1703964","url":null,"abstract":"We are pleased that Sameer Padhye and Neelesh Dahanukar (hereafter referred to as PN) agree that there is a significant need for DNA barcode data on this important group of organisms, and that a major goal of the paper (Abhyankar et al. 2019) was to initiate an effort to fill this gap. PN, do however, make other comments questioning other points made in the paper, and here we will attempt to address each of them","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 1","pages":"42-43"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1703964","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37475451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An enigmatic population of <i>Alsodes</i> (Anura, Alsodidae) from the Andes of central Chile with three species-level mitochondrial lineages.","authors":"Claudio Correa,&nbsp;Jesús Morales,&nbsp;Constantino Schussler,&nbsp;Juan C Ortiz","doi":"10.1080/24701394.2019.1704744","DOIUrl":"https://doi.org/10.1080/24701394.2019.1704744","url":null,"abstract":"<p><p>The genus <i>Alsodes</i> is the most diverse amphibian taxon of southern Andes, where a series of endemic species occur from north to south (∼33° to 48°30'S), mainly on the western slopes (Chile), with little overlap of geographic ranges. In this study, we describe a new population of <i>Alsodes</i> from the western slopes of the Andes (Fundo El Rosario, 36°52'S), located in a section of this mountain range where it is not clear which congeneric species inhabit. Phylogenetic analyses show that this population presents a mix of mitochondrial DNA related to three nominal species, <i>A. hugoi</i>, <i>A. igneus</i> and <i>A. pehuenche</i>, although the last is mainly present. However, the population presents external characteristics attributable to <i>A. hugoi</i> and <i>A. igneus</i>, differing notably from <i>A. pehuenche</i>. The population inhabits a stream surrounded by temperate deciduous forests at 1130 m elevation, an environment similar to that of <i>A. hugoi</i> and <i>A. igneus</i>, but very different from that of <i>A. pehuenche</i> (high Andean steppe environments between 2000 and 2500 m). This finding represents an enigma from the evolutionary and biogeographical point of view, because it might involve hybridization and/or introgression phenomena between more than two species. As the taxonomic status of this population cannot be determined with the available genetic and morphological evidence, we speculate about its probable origin, taking into account the scarce knowledge of the geographic distributions of the species of <i>Alsodes</i> in the area.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"31 1","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1704744","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37514181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pristimantis</i> in the Eastern Brazilian Amazon: DNA barcoding reveals underestimated diversity in a megadiverse genus.","authors":"Elciomar Araújo De Oliveira,&nbsp;Marcos Penhacek,&nbsp;Karen Larissa Auzier Guimarães,&nbsp;Gessica Amorim do Nascimento,&nbsp;Luís Reginaldo Ribeiro Rodrigues,&nbsp;Emil José Hernández-Ruz","doi":"10.1080/24701394.2019.1634696","DOIUrl":"https://doi.org/10.1080/24701394.2019.1634696","url":null,"abstract":"<p><p>The genus <i>Pristimantis</i> has the highest species diversity among all terrestrial vertebrates, with most species observed in the Andean region and the Guiana Shield. Even with the recent description of a new species, only <i>P. latro</i>, <i>P. dundeei</i> and <i>P. zimmermanae</i> occur in the south of the Amazon River. The lack of taxonomists specialized in the field leads to the propagation of dubious terminologies (e.g. <i>Pristimantis</i> sp1, <i>Pristimantis</i> sp2, <i>P.</i> aff. <i>Fenestratus</i> and <i>P.</i> gr. c<i>onspicillatus</i>) or even misidentification of species, resulting in erroneous species distributions. In this study, we applied the Automatic Barcode Gap Discovery (ABGD) algorithm for the delimitation of candidate species and values of genetic distances using the mitochondrial marker Cytochrome Oxidase Subunit I (COI), proposed in the barcode methodology, where values greater than 10% are considered as indicative of different species. We found large genetic distances between <i>P. latro</i> and <i>Pristimantis</i> sp1 Unconfirmed Candidate Species - UCS1 (21%), and between <i>P. altamazonicus</i> and <i>Pristimantis</i> sp2 UCS2 (14%). The ABGD method recognized UCS1 and UCS2 as distinct species. <i>Pristimantis</i> sp. UCS1 and UCS2 in the east of the Brazilian Amazon are indicated as candidate species. We suggest greater sampling of <i>Pristimantis</i> sp. UCS1 and UCS2, integrating morphology and bioacoustics to solve the taxonomic status in the east of the Brazilian Amazon.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"30 6","pages":"731-738"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1634696","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37386837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prey, populations, and the pleistocene: evidence for low COI variation in a widespread North American leech.","authors":"Joseph Mack,&nbsp;Danielle de Carle,&nbsp;Sebastian Kvist","doi":"10.1080/24701394.2019.1634698","DOIUrl":"https://doi.org/10.1080/24701394.2019.1634698","url":null,"abstract":"<p><p><i>Placobdella rugosa</i> has long presented challenges to leech biologists. Its extreme morphological variability and similarity to some congeneric species has confounded classification for over a century. Recent molecular analyses revealed a surprising lack of genetic variation among morphologically disparate, geographically widespread specimens of <i>P. rugosa</i>. Given the lack of any obvious mechanism by which this species could disperse between distant habitats, it was expected that widespread populations would be genetically isolated from each other. In the present study, we investigate the relationship between geographic distance and genetic diversity in <i>P. rugosa</i> using COI sequences from specimens collected across Canada and the United States. Although we find preliminary evidence for a barrier to gene flow between eastern and western collecting localities, our vastly expanded dataset largely corroborates prior studies, showing minimal phylogeographic signal among the sequences and negligible levels of genetic isolation by distance. A recent range expansion following the last ice age and/or host-mediated dispersal are discussed as potential explanations for this unexpected phylogeographic pattern.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"30 6","pages":"749-763"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1634698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37391845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of leopard frogs (Anura: Ranidae: <i>Lithobates</i>) distributed in some localities of the Southern Mexican Plateau using mitochondrial DNA sequences.","authors":"Daniel Ochoa-Vázquez,&nbsp;Rogelio Rosas-Valdez,&nbsp;Elizabeth A Martínez-Salazar,&nbsp;Oscar Flores-Villela","doi":"10.1080/24701394.2019.1634697","DOIUrl":"https://doi.org/10.1080/24701394.2019.1634697","url":null,"abstract":"<p><p>Species of the genus <i>Lithobates</i> are difficult to identify, especially on the <i>'Rana pipiens'</i> complex, because the morphological differences among the species are often subtle. In this study, we used 12S ribosomal RNA gene partial sequences to identify specimens of leopard frogs from some new localities (not sampled on previous studies) of the Southern Mexican Plateau, to carry out a phylogenetic analysis, and also a morphometric analysis of some morphological features were analyzed to evaluate their morphological variation. A phylogenetic analysis using partial sequences of 12S rDNA mitochondrial gene for 31 samples was carried out using Bayesian Inference, Maximum Parsimony, and Maximum Likelihood. In addition, 20 morphological lineal measurements from 97 specimens were analyzed by principal component analysis (PCA) to evaluate if the frogs studied are conspecific. Partial sequences of the 12S rDNA obtained from frogs distributed in the Southern Mexican Plateau show two haplotypes with a divergence of 0.4%. Phylogenetic hypothesis shows an exclusive group with a previous sequence of <i>Lithobates montezumae</i>. The PCA indicates that variables are not linearly correlated and specimens belong to a single group. Evidence found, let us consider that specimens studied belong to one species of the <i>Lithobates montezumae</i> subgroup, distributed principally in the Southern Mexican Plateau.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"30 6","pages":"739-748"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1634697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37115472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population differentiation and genetic diversity of endangered <i>Brachymystax tsinlingensis</i> Li between Yangtze River and Yellow River in China based on mtDNA.","authors":"Jian Shao,&nbsp;Dongmei Xiong,&nbsp;Zhipeng Chu,&nbsp;Jinming Wu,&nbsp;Hao Du,&nbsp;Feng Wang,&nbsp;Sien Wen,&nbsp;Qiwei Wei","doi":"10.1080/24701394.2019.1572130","DOIUrl":"https://doi.org/10.1080/24701394.2019.1572130","url":null,"abstract":"<p><p><i>Brachymystax tsinlingensis</i> Li, distributed scatteredly in Qinling Mountains of China, is an ideal material for studies of conservation and phylogeography. In the present study, the genetic variations and phylogeographical patterns of <i>B. tsinlingensis</i> collecting from the Yangtze River (<i>n</i> = 30) and the Yellow River (<i>n</i> = 70) were compared based on 1082-1163 bp control region with partial tRNA and 912 bp cyt <i>b</i> partial sequence of mtDNA. Analysis of variance indicated that a high proportion of the total genetic variance (96.03%) was distributed among populations, supporting strong geographic structuring of mtDNA polymorphism. Ten haplotypes were assigned to two clades that were related to geographic regions. No haplotype shared between geographic populations from the Yangtze River and the Yellow River, and two clades related to geographic regions can be clearly found from phylogenetic analysis by Bayesian approach, one from individuals in the Yangtze River and the other from individuals in the Yellow River, which probably indicated artificial carrying not happened historically. The mitochondrial results revealed an obvious differentiation of genetic structure of <i>B. tsinlingensis</i> between populations of Yellow River and Yangtze River, and the estimated diverge time around 0.20 MY. It was suggested that each of the two evolutionarily distinct groups of <i>B. tsinlingensis</i> should be protected and crossing individuals between two river systems should be avoided for conservation purpose.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"30 5","pages":"695-701"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1572130","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37405666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA barcoding of the rodent genus <i>Oligoryzomys</i> (Cricetidae: Sigmodontinae): mitogenomic-anchored database and identification of nuclear mitochondrial translocations (Numts).","authors":"Marcos O R da Cruz,&nbsp;Marcelo Weksler,&nbsp;Cibele R Bonvicino,&nbsp;Alexandra M R Bezerra,&nbsp;Francisco Prosdocimi,&nbsp;Carolina Furtado,&nbsp;Lena Geise,&nbsp;François Catzeflis,&nbsp;Benoit de Thoisy,&nbsp;Luiz F B de Oliveira,&nbsp;Claudia Silva,&nbsp;João Alves de Oliveira","doi":"10.1080/24701394.2019.1622692","DOIUrl":"https://doi.org/10.1080/24701394.2019.1622692","url":null,"abstract":"<p><p>DNA barcoding has become a standard method for species identification in taxonomically complex groups. An important step of the barcoding process is the construction of a library of voucher-based material that was properly identified by independent methods, free of inaccurate identification, and paralogs. We provide here a <i>cytochrome oxidase I</i> (<i>mt-Co1</i>) DNA barcode database for species of the genus <i>Oligoryzomys</i>, based on type material and karyotyped specimens, and anchored on the mitochondrial genome of one species of <i>Oligoryzomys</i>, <i>O. stramineus</i>. To evaluate the taxonomic determination of new COI sequences, we assessed species intra/interspecific genetic distances (barcode gap), performed the General Mixed Yule Coalescent method (GMYC) for lineages' delimitation, and identified diagnostic nucleotides for each species of <i>Oligoryzomys</i>. Phylogenetic analyses of <i>Oligoryzomys</i> were performed on 2 datasets including 14 of the 23 recognized species of this genus: a <i>mt-Co1</i> only matrix, and a concatenated matrix including <i>mt-Co1</i>, <i>cytochrome b</i> (<i>mt-Cytb</i>), and <i>intron 7 of the nuclear fibrinogen beta chain gene</i> (<i>i7Fgb</i>). We recovered nuclear-mitochondrial translocated (Numts) pseudogenes on our samples and identified several published sequences that are cases of Numts. We analyzed the rate of non-synonymous and synonymous substitution, which were higher in Numts in comparison to mtDNA sequences. GMYC delimitations and DNA barcode gap results highlight the need for further work that integrate molecular, karyotypic, and morphological analyses, as well as additional sampling, to tackle persistent problems in the taxonomy of <i>Oligoryzomys</i>.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"30 5","pages":"702-712"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1622692","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37077069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic analysis reveals fraud in fillets from the \"Gurijuba\" <i>Sciades parkeri</i> (Ariidae - Siluriformes): a vulnerable fish in Brazilian Coastal Amazon.","authors":"Grazielle Gomes,&nbsp;Rafael Correa,&nbsp;Ivana Veneza,&nbsp;Raimundo da Silva,&nbsp;Danillo da Silva,&nbsp;Josy Miranda,&nbsp;Iracilda Sampaio","doi":"10.1080/24701394.2019.1622694","DOIUrl":"https://doi.org/10.1080/24701394.2019.1622694","url":null,"abstract":"<p><p>The utilization of molecular tools for the certification of fishery products has been increasing over the last years. In general, economically important species are replaced by less valuable species, characterizing a commercial fraud. We evaluated the authenticity of 107 frozen fillets tagged as Gurijuba (<i>Sciades parkeri</i>) and Uritinga (<i>Sciades proops</i>) from local markets in northern amazon coast by sequencing two mitochondrial genes: Cytochrome oxidase subunit I and cytochrome b (Cyt b). About 16% of fillets putatively related to <i>S. parkeri</i> were replaced by <i>S. proops</i>. The Gurijuba faces high fishing pressure, being currently listed by the International Union for Conservation of Nature as vulnerable. Forensic analysis with DNA markers, proved to be highly efficient in the discrimination of the processed seafood products, providing unequivocal identification of species, revealing commercial fraud in the fillets of the Gurijuba, and revealing the utility of Cytb sequences as barcode in fishes.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"30 5","pages":"721-729"},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/24701394.2019.1622694","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37319584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}