Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献

The complete chloroplast genome of Mesosphaerum suaveolens (L.) Kuntze and phylogenetic position in the Lamiaceae family. 中叶植物（Mesosphaerum suaveolens）叶绿体全基因组的研究紫紫属植物及其在紫紫科的系统发育地位。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-10-11 DOI: 10.1080/24701394.2026.2743696

Qing Du, Yihong Luo, Chang Liu

{"title":"The complete chloroplast genome of Mesosphaerum suaveolens (L.) Kuntze and phylogenetic position in the Lamiaceae family.","authors":"Qing Du, Yihong Luo, Chang Liu","doi":"10.1080/24701394.2026.2743696","DOIUrl":"https://doi.org/10.1080/24701394.2026.2743696","url":null,"abstract":"The complete chloroplast genome of Mesosphaerum suaveolens is 152,555 base pairs long and has a typical quadripartite structure. It contains 132 genes, including 13 cis-splicing genes, 2 trans-splicing genes (rps12), and 8 tRNA genes with introns. A total of 47 simple sequence repeats (SSRs) were identified: 41 mononucleotide repeats (19 A, 1 C, 21 T), 4 dinucleotide repeats (2 TA, 2 AT), and 2 complex repeats (21 bp and 81 bp). The genes rps19 (LSC and IRb), ndhF (SSC and IRb), and ycf1 (SSC and IRa) are located at the borders between two regions. The most frequently used codons and amino acids are UGU (Cys), GGU (Gly), UUA (Leu), and UUU (Phe). Phylogenetic analysis indicates that the chloroplast genome of M. suaveolens is closely related to Ocimum gratissimum, and that the Isodon Hanceola clade forms a major clade comprising 19 species across 11 Lamiaceae genera. These findings support species identification, evolutionary research, and conservation genetics for M. suaveolens and related medicinal plants.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"1-9"},"PeriodicalIF":0.6,"publicationDate":"2026-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149169491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and population structure of Mierspenaeopsis hardwickii (Decapoda: penaeidae) along the coastal waters of Peninsular Malaysia based on the mitochondrial COI gene. 基于线粒体COI基因的马来西亚半岛沿海Mierspenaeopsis hardwickii（十足目：对虾科）遗传多样性和种群结构

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-10-07 DOI: 10.1080/24701394.2026.2739856

Muhammad Amiruddin Afiq Suhailan, Chien-Hui Yang, Jamsari Amirul Firdaus Jamaluddin, Hong Chiun Lim, Noor Adelyna Mohammed Akib, Muzzalifah Abd Hamid, Amirah Hurzaid

{"title":"Genetic diversity and population structure of Mierspenaeopsis hardwickii (Decapoda: penaeidae) along the coastal waters of Peninsular Malaysia based on the mitochondrial COI gene.","authors":"Muhammad Amiruddin Afiq Suhailan, Chien-Hui Yang, Jamsari Amirul Firdaus Jamaluddin, Hong Chiun Lim, Noor Adelyna Mohammed Akib, Muzzalifah Abd Hamid, Amirah Hurzaid","doi":"10.1080/24701394.2026.2739856","DOIUrl":"https://doi.org/10.1080/24701394.2026.2739856","url":null,"abstract":"Mierspenaeopsis hardwickii (Miers, 1878) is a commercially important penaeid shrimp widely distributed along the coastal waters of Peninsular Malaysia. Despite its economic significance, information on its population structure remains limited. This study investigated the genetic diversity and population structure of M. hardwickii using the mitochondrial cytochrome oxidase subunit 1 (COI) gene. A total of 108 individuals of M. hardwickii were collected from seven localities representing two geographical regions: the Strait of Malacca (SOM) and the South China Sea (SCS). Analysis of approximately 1,100 bp COI sequences revealed low haplotype diversity (h = 0.133-0.257) and low nucleotide diversity (π = 0.0003-0.0056) across the sampled populations. Analysis of molecular variance (AMOVA) revealed that 68.71% of the total genetic variation was attributable to differences between the SOM and SCS regions, indicating strong regional genetic differentiation. This finding was further supported by a high and significant overall ΦST value (ΦST = 0.6732, p < 0.001). Phylogenetic and haplotype network analyses revealed two geographically differentiated mitochondrial groups corresponding to the SOM and SCS regions. The observed genetic structure may reflect historical biogeographic processes associated with Quaternary sea-level fluctuations and the emergence of the Sunda Shelf as a barrier to gene flow. These findings provide important baseline information for fisheries management and support the precautionary consideration of the SOM and SCS populations as provisional management units.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"1-10"},"PeriodicalIF":0.6,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149129348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and population structure of crescent perch, Terapon jarbua (Forsskål, 1775), along the Indian coast: insights from mitochondrial D-loop analysis. 印度沿海新月鲈Terapon jarbua （forsskamatl, 1775）的遗传多样性和种群结构：来自线粒体d环分析的见解。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-10-01 Epub Date: 2026-09-05 DOI: 10.1080/24701394.2026.2723762

Shajahan Shahana, Susanthi Silpa, Murugesan SriHari, Shashi Bhushan, Karankumar Kishorkumar Ramteke, Giri Bhavan Sreekanth, Annam Pavan-Kumar

{"title":"Genetic diversity and population structure of crescent perch, Terapon jarbua (Forsskål, 1775), along the Indian coast: insights from mitochondrial D-loop analysis.","authors":"Shajahan Shahana, Susanthi Silpa, Murugesan SriHari, Shashi Bhushan, Karankumar Kishorkumar Ramteke, Giri Bhavan Sreekanth, Annam Pavan-Kumar","doi":"10.1080/24701394.2026.2723762","DOIUrl":"10.1080/24701394.2026.2723762","url":null,"abstract":"The crescent perch, Terapon jarbua, is a medium-sized, euryhaline fish of ecological and economic importance, contributing to demersal fisheries and the marine ornamental fish trade in India. Genetic diversity and population structure of T. jarbua along the Indian coast were assessed using 145 partial sequences (506 bp) of the mitochondrial D-loop region. Populations exhibited high haplotype and nucleotide diversity. A total of 96 haplotypes were identified, with several shared among geographically distant populations. The results revealed high within-population variation and broad genetic connectivity of T. jarbua along the Indian coast, with evidence of partial genetic structuring and low-to-moderate differentiation in some pairwise comparisons. Neutrality tests and mismatch-distribution analyses provided suggestive evidence of historical demographic expansion. High genetic diversity and demographic patterns consistent with a possible historical expansion suggest a complex demographic history, including a possible population expansion during the Pleistocene epoch. These findings provide baseline information for future genetic assessments and the sustainable management of T. jarbua in Indian waters.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"171-181"},"PeriodicalIF":0.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148898564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the complete mitochondrial genome of the common Mexican mustached bat Pteronotus mexicanus. 墨西哥八字蝠全线粒体基因组的研究。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-10-01 Epub Date: 2026-08-07 DOI: 10.1080/24701394.2026.2695112

Jorge Alemán-Silva, Jorge Ortega, Edgar G Gutiérrez, Carlos F Calderón-Palapa, J Antonio Baeza

{"title":"Characterization of the complete mitochondrial genome of the common Mexican mustached bat Pteronotus mexicanus.","authors":"Jorge Alemán-Silva, Jorge Ortega, Edgar G Gutiérrez, Carlos F Calderón-Palapa, J Antonio Baeza","doi":"10.1080/24701394.2026.2695112","DOIUrl":"10.1080/24701394.2026.2695112","url":null,"abstract":"This study presents the characterization of the complete mitochondrial genome of Pteronotus mexicanus, an endemic species from Mexico and a representative of the family Mormoopidae. The mitogenome was assembled de novo using the program GetOrganelle, and gene annotation was performed with the software MITOS2. The newly assembled mitogenome has a total length of 16,762 base pairs and exhibits the typical mammalian gene organization: 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a non-coding region commonly referred to as the control region. The nucleotide composition of the mitogenome was A = 33.10%, T = 25.29%, C = 27.89%, and G = 13.72%, with an overall A + T content of 58.39%. Codon usage analysis revealed preferences consistent with the adenine- and thymine-rich composition characteristic of vertebrate mitochondrial DNA. Ka/Ks ratio values estimated indicated purifying selective pressure on the protein-coding genes. Predicted secondary structures of tRNAs exhibited the typical cloverleaf configuration, except for trnS1, which lacks the DHU arm-a common feature among mammals. The phylogenetic position of the common Mexican mustached bat supports the monophyly of the genus within the superfamily Noctilionoidea. This study represents the first comprehensive description of the mitogenome of P. mexicanus and provides a valuable molecular resource for future research on the systematics, phylogeny, and population genetics of Neotropical bats.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"149-158"},"PeriodicalIF":0.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148690407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and lineage divergence of Palaemon modestus in the Three Gorges Reservoir area. 三峡库区古蠓的遗传多样性和谱系分化。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-10-01 Epub Date: 2026-08-15 DOI: 10.1080/24701394.2026.2716017

Dong Wang, Meixi Huang, Yining Cao, Hongyan Liu, Dongdong Zhai, Fei Xiong

{"title":"Genetic diversity and lineage divergence of Palaemon modestus in the Three Gorges Reservoir area.","authors":"Dong Wang, Meixi Huang, Yining Cao, Hongyan Liu, Dongdong Zhai, Fei Xiong","doi":"10.1080/24701394.2026.2716017","DOIUrl":"10.1080/24701394.2026.2716017","url":null,"abstract":"To assess the genetic diversity and population structure of Palaemon modestus in the Three Gorges Reservoir (TGR), we analyzed mitochondrial COI sequences from five populations and further evaluated the two mitochondrial clades revealed by COI using Cyt b, D-loop, and nuclear 28S rDNA markers. The TGR populations showed high genetic diversity, with a haplotype diversity of 0.920 and a nucleotide diversity of 1.400%. Bayesian phylogenetic analysis and the COI haplotype network separated 54 haplotypes into TGR Clade A and TGR Clade B, with 11 mutational steps between them; Cyt b and D-loop phylogenies also supported the two-clade pattern. However, 28S rDNA sequences were identical between the clades, and ABGD assigned all COI haplotypes to a single molecular operational taxonomic unit. The overall FST among populations was 0.067; when analysis was restricted to Clade A individuals, the overall FST remained 0.064, indicating that population differentiation was not solely attributable to the uneven distribution of Clade B. Overall, P. modestus in the TGR maintains high genetic diversity and shows detectable population differentiation. The available evidence supports interpreting TGR Clades A and B as deeply divergent mitochondrial lineages within P. modestus.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"159-170"},"PeriodicalIF":0.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148762804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete mitogenome of Plagiognathops microlepis and its phylogenetic position within the subfamily Xenocyprinae (Cypriniformes: Cyprinidae). 小鳞斜齿鲷有丝分裂全基因组及其在异鲤亚科中的系统发育地位（鲤形：鲤科）

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-10-01 Epub Date: 2026-09-02 DOI: 10.1080/24701394.2026.2725715

Huiming Zhou, Guoyong Xiong, Jiaxin Yuan, Menglu Li, Zhiying Tao

{"title":"Complete mitogenome of Plagiognathops microlepis and its phylogenetic position within the subfamily Xenocyprinae (Cypriniformes: Cyprinidae).","authors":"Huiming Zhou, Guoyong Xiong, Jiaxin Yuan, Menglu Li, Zhiying Tao","doi":"10.1080/24701394.2026.2725715","DOIUrl":"10.1080/24701394.2026.2725715","url":null,"abstract":"The complete mitochondrial genome of Plagiognathops microlepis was sequenced using Illumina NovaSeq and PacBio Sequel II platforms. The circular mitogenome is 16,632 bp in length and contains 37 genes: 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a non-coding control region (D-loop). The genome exhibits an AT content of 56.7%, with an AT skew of 0.104 and a GC skew of -0.255. COX1 initiates with GTG and CYTB with ATA, whereas the remaining 11 PCGs employ ATG; four PCGs (COX2, COX3, ND3, and ND4) terminate with incomplete stop codons (T or TA). Relative synonymous codon usage (RSCU) analysis revealed 27 preferred codons (RSCU > 1), with a strong bias toward A- and T-ending codons. All 22 tRNAs display conventional cloverleaf secondary structures. Maximum likelihood (ML), Bayesian inference (BI) and Neighbor joining (NJ) analyses consistently recovered P. microlepis as sister species to Xenocypris davidi (NJ bootstrap = 1, ML UFBoot = 67, BI posterior probability = 0.977), confirming its phylogenetic position within the genus Xenocypris. These findings provide molecular evidence for the taxonomic placement of P. microlepis and genomic resources for species delimitation, conservation genetics and evolutionary studies within Xenocyprinae.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"182-195"},"PeriodicalIF":0.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148876947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two ancient mitochondrial genomes belonging to the Siberian roe deer (Capreolus pygargus) from Northern China reveal regional genetic structure of populations. 中国北方西伯利亚狍子（Capreolus pygargus）的两个古线粒体基因组揭示了种群的区域遗传结构。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-09-30 DOI: 10.1080/24701394.2026.2739858

Guangjie Song, Xinyan Zhang, Ningning Dong, Jilin Lian, Dawei Cai

{"title":"Two ancient mitochondrial genomes belonging to the Siberian roe deer (Capreolus pygargus) from Northern China reveal regional genetic structure of populations.","authors":"Guangjie Song, Xinyan Zhang, Ningning Dong, Jilin Lian, Dawei Cai","doi":"10.1080/24701394.2026.2739858","DOIUrl":"https://doi.org/10.1080/24701394.2026.2739858","url":null,"abstract":"Ancient mitochondrial DNA informs the evolutionary history and regional differentiation of prehistoric wildlife. Two ancient Siberian roe deer specimens, XTS03G from the Xiaotangshan site in eastern Inner Mongolia (ca. 3,500-3,300 BP) and SZC01S from the Shizhaocun site in southeastern Gansu (ca. 6,000 BP), were analyzed by high-throughput sequencing. The datasets exhibited characteristic ancient DNA damage patterns, supporting sample authenticity. A maximum-likelihood phylogenetic tree demonstrated that XTS03G belonged to a previously described subclade P2, whereas SZC01S belonged to another previously described subclade P1 and clustered with previously published ancient samples from northwestern China, forming a distinct prehistoric northwestern Chinese cluster. Our results indicate regional differentiation in the maternal genetic structure of ancient Siberian roe deer in northern China. Nucleotide diversity was slightly higher in ancient than modern populations. Bayesian skyline plot analysis suggested population expansion before approximately 12,000 BP, relative stability with a slight decline between approximately 12,000 and 5,000 BP, and a more pronounced decline after approximately 5,000 BP toward modern times. Our findings provide new molecular evidence for the genetic history of Siberian roe deer in northern China and highlight the value of integrating ancient and modern genetic data for understanding long-term wildlife evolution and conservation.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"1-9"},"PeriodicalIF":0.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity, population genetic structure and demographic history of two actiniarian species (Anthopleura xanthogrammica and Diadumene lineata) along the Chinese coastline. 中国沿海两种放线虫种（Anthopleura xanthogrammica和Diadumene lineata）的遗传多样性、种群遗传结构和人口统计学历史。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-06-25 DOI: 10.1080/24701394.2026.2691597

Xinyuan Cai, Yue Fang, Yujie Song, Xiaoxin Bi, Lei Xu, Ling Huang, Meidong Jing

{"title":"Genetic diversity, population genetic structure and demographic history of two actiniarian species (Anthopleura xanthogrammica and Diadumene lineata) along the Chinese coastline.","authors":"Xinyuan Cai, Yue Fang, Yujie Song, Xiaoxin Bi, Lei Xu, Ling Huang, Meidong Jing","doi":"10.1080/24701394.2026.2691597","DOIUrl":"10.1080/24701394.2026.2691597","url":null,"abstract":"Anthopleura xanthogrammica and Diadumene lineata have considerable pharmaceutical potential, but their distribution and population genetics along the Chinese coastline remain poorly understood. We collected 162 samples of the two species based on external morphology from nine intertidal sites. Subsequent analyses of the mitochondrial COI segment (607 bp) confirmed 143 A. xanthogrammica individuals across all nine localities, and only 19 D. lineata individuals restricted to three localities. For A. xanthogrammica, genetic diversity, phylogenetic relationships, population structure and demographic history were analyzed using combined mitochondrial segments (COI + 16S rRNA: 1,055 bp) and nuclear segments (28S rRNA: 316 bp; ITS: 658 bp). This species showed high genetic polymorphism. Mitochondrial DNA data (neutrality tests, mismatch distributions, haplotype network) suggested a historical and mild population expansion, while phylogenetic and molecular variance analyses indicated limited gene flow and high genetic differentiation among geographic populations. Population structure supported three management units for the conservation of this species. For D. lineata, we attempted genetic diversity and neutrality tests using the same molecular markers, but the results were unreliable due to the small sample size. Future studies should identify more suitable sampling sites and use a larger sample size to assess its genetic polymorphism and population structure.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"75-86"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148321073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mitochondrial genome of the sun anemone shrimp Periclimenes rathbunae (Decapoda: Caridea: Palaemonidae). 太阳海葵对虾的线粒体基因组（十足目：虾总目：海葵科）。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-06-29 DOI: 10.1080/24701394.2026.2695116

Kareem Hussein, J Antonio Baeza

{"title":"The mitochondrial genome of the sun anemone shrimp Periclimenes rathbunae (Decapoda: Caridea: Palaemonidae).","authors":"Kareem Hussein, J Antonio Baeza","doi":"10.1080/24701394.2026.2695116","DOIUrl":"10.1080/24701394.2026.2695116","url":null,"abstract":"The sun anemone shrimp, Periclimenes rathbunae (Decapoda: Palaemonidae), is a small, translucent shrimp broadly distributed across the tropical Western Atlantic that typically inhabits sea anemones in shallow waters. Recent trading of the host sea anemones in the ornamental industry has likely increased stress on the population health of this shrimp. To aid in conservation efforts, the complete mitochondrial genome of P. rathbunae has been assembled and characterized in detail. The mitochondrial genome of P. rathbunae is 15,809 bp long and includes 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes (12S and 16S rRNA), and a 976 bp control region. The gene order is identical to that reported for two other species in family Palaemonidae, but different from the ancestral decapod ground pattern. The overall A + T content is 67.8%, and the codon usage of PCGs exhibits a strong preference for A + T-rich codons. All 13 PCGs exhibited purifying selection, with the strongest selection exhibited in cox1 and the weakest exhibited in atp8. All tRNA genes exhibited the typical cloverleaf secondary structures except for trnT and trnL2, which were missing the TC-loop, trnR missing the D-loop, and trnS1 missing the dihydroxyuridine (DHU) arm. The control region exhibited a high A + T content (74.2%), four dinucleotide microsatellite repeats, a single tandem repeat, and multiple hairpin loops in the predicted Minimum Free Energy (MFE) and Centroid secondary structures. The newly assembled mitochondrial genome will aid in the identification of P. rathbunae and support programs based on environmental DNA (eDNA).","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"87-94"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148347662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative mitochondrial genome analysis of two biting midges, Dasyhelea ludingensis and Forcipomyia (Lasiohelea) taiwana (Diptera: Ceratopogonidae). 两种吸血蠓卢氏蠓和台湾蠓的线粒体基因组比较分析（双翅目：蠓科）。

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Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-07-18 DOI: 10.1080/24701394.2026.2702528

Xiaohong Jiang, Yao Xie, Guojiang Zhang, Fuchun Wei, Jing Zhu, Mei Wang, Lin Ao, Qiongyou Liu

{"title":"Comparative mitochondrial genome analysis of two biting midges, Dasyhelea ludingensis and Forcipomyia (Lasiohelea) taiwana (Diptera: Ceratopogonidae).","authors":"Xiaohong Jiang, Yao Xie, Guojiang Zhang, Fuchun Wei, Jing Zhu, Mei Wang, Lin Ao, Qiongyou Liu","doi":"10.1080/24701394.2026.2702528","DOIUrl":"10.1080/24701394.2026.2702528","url":null,"abstract":"Though the family Ceratopogonidae is a species-rich group (6346 extant species in 2025) within the Diptera, the complete mitochondrial genomes (mitogenomes) have been characterized for a small number of species. In this study, we sequenced and characterized the complete mitogenomes of Dasyhelea ludingensis (15,687 bp) and Forcipomyia (Lasiohelea) taiwana (17,315 bp) to explore their genomic structure and phylogenetic relationships. Each mitogenome exhibited the typical insect structure: 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and a control region, maintaining the ancestral insect gene arrangement. All PCGs used typical ATN start codons, except for COX1 in D. ludingensis, which was putatively initiated by the atypical AAC codon. Both mitogenomes exhibited a strong A + T bias, with A + T contents of 78.29% in D. ludingensis and 78.89% in F. taiwana, respectively, which affects codon usage and amino acid (AA) composition. Regarding tRNA secondary structures, trnS1-GCU in F. taiwana lacked the dihydrouridine (DHU) arm and contained an unpaired adenine residue in the anticodon stem. Phylogenetic analyses confirmed the taxonomic status of D. ludingensis and F. taiwana at the molecular level. This study provides essential genomic resources for future studies in systematics, phylogenetics, and population genetics of biting midges.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"119-129"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0