Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献_第2页

Comparative mitochondrial genome analysis of two biting midges, Dasyhelea ludingensis and Forcipomyia (Lasiohelea) taiwana (Diptera: Ceratopogonidae). 两种吸血蠓卢氏蠓和台湾蠓的线粒体基因组比较分析（双翅目：蠓科）。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-07-18 DOI: 10.1080/24701394.2026.2702528

Xiaohong Jiang, Yao Xie, Guojiang Zhang, Fuchun Wei, Jing Zhu, Mei Wang, Lin Ao, Qiongyou Liu

{"title":"Comparative mitochondrial genome analysis of two biting midges, Dasyhelea ludingensis and Forcipomyia (Lasiohelea) taiwana (Diptera: Ceratopogonidae).","authors":"Xiaohong Jiang, Yao Xie, Guojiang Zhang, Fuchun Wei, Jing Zhu, Mei Wang, Lin Ao, Qiongyou Liu","doi":"10.1080/24701394.2026.2702528","DOIUrl":"10.1080/24701394.2026.2702528","url":null,"abstract":"Though the family Ceratopogonidae is a species-rich group (6346 extant species in 2025) within the Diptera, the complete mitochondrial genomes (mitogenomes) have been characterized for a small number of species. In this study, we sequenced and characterized the complete mitogenomes of Dasyhelea ludingensis (15,687 bp) and Forcipomyia (Lasiohelea) taiwana (17,315 bp) to explore their genomic structure and phylogenetic relationships. Each mitogenome exhibited the typical insect structure: 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and a control region, maintaining the ancestral insect gene arrangement. All PCGs used typical ATN start codons, except for COX1 in D. ludingensis, which was putatively initiated by the atypical AAC codon. Both mitogenomes exhibited a strong A + T bias, with A + T contents of 78.29% in D. ludingensis and 78.89% in F. taiwana, respectively, which affects codon usage and amino acid (AA) composition. Regarding tRNA secondary structures, trnS1-GCU in F. taiwana lacked the dihydrouridine (DHU) arm and contained an unpaired adenine residue in the anticodon stem. Phylogenetic analyses confirmed the taxonomic status of D. ludingensis and F. taiwana at the molecular level. This study provides essential genomic resources for future studies in systematics, phylogenetics, and population genetics of biting midges.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"119-129"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148498391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal genetic structure and demographic patterns of the mitre squid Uroteuthis chinensis in the Formosa Bank inferred from mitochondrial DNA. 基于线粒体DNA的中华尾乌贼时空遗传结构及种群特征分析。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-07-09 DOI: 10.1080/24701394.2026.2696927

Ping Wen, Shuiqing Wu, Leibin Li, Shaopeng Guo, Denggao Qiu, Jiandi Cai, Minjie Fang

{"title":"Spatiotemporal genetic structure and demographic patterns of the mitre squid Uroteuthis chinensis in the Formosa Bank inferred from mitochondrial DNA.","authors":"Ping Wen, Shuiqing Wu, Leibin Li, Shaopeng Guo, Denggao Qiu, Jiandi Cai, Minjie Fang","doi":"10.1080/24701394.2026.2696927","DOIUrl":"10.1080/24701394.2026.2696927","url":null,"abstract":"Uroteuthis chinensis, a commercially significant species distributed across the western Pacific, plays a vital role in marine fishery resources, accounting for up to 90% of the loliginid catch in several regions of China. However, there have been few genetic studies on this species exploring the relationship between its life history and genetic diversity. Therefore, this study aimed to address the genetic diversity and population structure of U. chinensis using 143 individuals collected from the coast of mainland China (Formosa Bank) based on the mitochondrial COI gene. Our results revealed high haplotype diversity (h = 0.906) and low nucleotide diversity in U. chinensis, a pattern consistent with recent demographic expansion following a period of reduced effective population size. Pairwise FST and analysis of molecular variance (AMOVA) revealed no significant genetic differentiation among the four sampling groups, suggesting an absence of detectable fine-scale spatiotemporal genetic structure within the Formosa Bank during the sampling period. In addition, demographic history analysis, based on Approximate Bayesian Computation (ABC), indicates that U. chinensis experienced a bottleneck event followed by a recent population expansion. These findings provide useful information for understanding the genetic diversity, demographic history, and sustainable management of this commercially important squid.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"95-105"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148414113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using simple molecular markers for the identification of billfish traded in the Eastern Tropical Pacific. 利用简单的分子标记对东热带太平洋交易的长嘴鱼进行鉴定。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-07-21 DOI: 10.1080/24701394.2026.2701086

Roy Francisco Saravia-Sánchez, Sebastián Ignacio Hernández-Muñoz, Sofía Ortega-García, José Francisco Domínguez-Contrera, Francisco Javier García-Rodríguez

{"title":"Using simple molecular markers for the identification of billfish traded in the Eastern Tropical Pacific.","authors":"Roy Francisco Saravia-Sánchez, Sebastián Ignacio Hernández-Muñoz, Sofía Ortega-García, José Francisco Domínguez-Contrera, Francisco Javier García-Rodríguez","doi":"10.1080/24701394.2026.2701086","DOIUrl":"10.1080/24701394.2026.2701086","url":null,"abstract":"Billfishes (Xiphiidae and Istiophoridae) are economically important species. Unfortunately, these fish are part of the bycatch in many fisheries and are subject to unregulated trade. Another problem is inadequate identification, as diagnostic features are often removed when they are captured. This research aimed to design protocols based on PCR-RFLPs and PCR-Multiplex to carry out a relatively rapid and robust identification of billfish species of commercial interest in the Eastern Tropical Pacific. In silico PCR-RFLP analysis allowed the selection of six restriction enzymes; the enzyme HinfI was the most effective for species discrimination, while PvuII was the least effective. For the PCR-Multiplex, it was observed that the primers designed in silico amplify approximate sizes for their respective species and allow the identification of the species through the difference in band sizes in the agarose gels. These tools were tested on tissues from commercial sources, where it was possible to identify three species of billfishes (Kajikia audax, Makaira nigricans, and Xiphias gladius) and two species from other groups (Nematistius pectoralis and Thunnus albacares). The application of the protocols was successful in fresh tissue and processed (smoked) tissue. These protocols, implemented together, allow for greater support in the genetic identification of billfishes.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"106-118"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148551818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HapNet: a new python package for automated population-aware haplotype network analysis and visualization. 一个新的python包，用于自动感知人口的单倍型网络分析和可视化。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-07-20 DOI: 10.1080/24701394.2026.2705539

Andrew A Davinack

{"title":"HapNet: a new python package for automated population-aware haplotype network analysis and visualization.","authors":"Andrew A Davinack","doi":"10.1080/24701394.2026.2705539","DOIUrl":"10.1080/24701394.2026.2705539","url":null,"abstract":"Haplotype networks are widely used in population genetics, phylogeography, and molecular ecology to visualize relationships among DNA sequences and summarize patterns of population connectivity. Existing tools vary in interface, algorithmic approach, input requirements, and output format. Here, I introduce HapNet, an open-source Python command-line package for constructing population-aware, minimum-spanning-tree-based haplotype graphs from aligned FASTA files. HapNet collapses identical sequences into haplotypes, calculates Hamming distances among haplotypes, constructs an MST-based graph, and generates publication-ready visualizations in which node size reflects haplotype frequency and pie-chart sectors indicate population composition. HapNet also produces machine-readable tabular outputs documenting haplotype membership, shared and private haplotypes, individual assignments, summary statistics, and run metadata. This version adds optional metadata input, phased diploid sequence support, individual-level genotype summaries, and label-free figure export. Utility is demonstrated using a published Hydroides dianthus COI dataset and a simulated phased diploid dataset.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"130-136"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148521561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial tRNA^Gly A10055G mutation contributes to non-syndromic hearing loss: a study of three Han Chinese pedigrees. 线粒体tRNAGly A10055G突变与非综合征性听力损失有关：三个汉族家系的研究

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-07-01 Epub Date: 2026-07-23 DOI: 10.1080/24701394.2026.2706011

Xiao-Xiang Chen, Xiu-Mei He, Yun Li, Bin Jiang, Xiang Zhou, Yu Ding

{"title":"Mitochondrial tRNAGly A10055G mutation contributes to non-syndromic hearing loss: a study of three Han Chinese pedigrees.","authors":"Xiao-Xiang Chen, Xiu-Mei He, Yun Li, Bin Jiang, Xiang Zhou, Yu Ding","doi":"10.1080/24701394.2026.2706011","DOIUrl":"10.1080/24701394.2026.2706011","url":null,"abstract":"Mutations in mitochondrial tRNA (mt-tRNA) genes are associated with non-syndromic hearing loss (NSHL), though their detailed molecular mechanisms and pathogenic potential remain unclear. In this study, we reported three Han Chinese pedigrees exhibiting maternally transmitted NSHL. Mitochondrial genomes from probands were sequenced and compared to revised Cambridge reference sequence (rCRS), followed by phylogenetic analysis to assess pathogenicity. We further established cybrid cell lines to measure mtDNA copy number, ATP levels, mitochondrial membrane potential (MMP), reactive oxygen species (ROS), and 8-OHdG, we also screened mutations in nuclear deafness genes including GJB2, GJB3, GJB6, and TRMU. Affected individuals displayed a wide range of age at onset (mean 40.5 years) of deafness. A homoplasmic m.A10055G mutation was identified in the tRNAGly gene, which disrupted a conserved base pair. Compared to controls, mutant cybrids showed significantly reduced mtDNA copy number, ATP, and MMP, along with increased levels of 8-OHdG and ROS, whereas no pathogenic nuclear variants were detected. Collectively, these findings indicated that the m.A10055G mutation contributed to NSHL pathogenesis by impairing mitochondrial functions, most likely through defective tRNA metabolism and by increasing oxidative stress.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"137-147"},"PeriodicalIF":0.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148564009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitogenomic characterization of Hoplia bomiensis Zeng (Coleoptera: Scarabaeidae: Melolonthinae) from Xizang, China. 西藏曾氏波米蚤（鞘翅目：金龟科：蜜蛉科）的有丝分裂基因组学特征。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-04-01 Epub Date: 2026-06-08 DOI: 10.1080/24701394.2026.2683394

Xiaoli Liu, Ziqiang Jing, Xueting Hong, Yang Hu, Jiamin Huang, Ruixi Huang, Xunhuan Ding, Tao Sun, Jiancheng Zang

{"title":"Mitogenomic characterization of Hoplia bomiensis Zeng (Coleoptera: Scarabaeidae: Melolonthinae) from Xizang, China.","authors":"Xiaoli Liu, Ziqiang Jing, Xueting Hong, Yang Hu, Jiamin Huang, Ruixi Huang, Xunhuan Ding, Tao Sun, Jiancheng Zang","doi":"10.1080/24701394.2026.2683394","DOIUrl":"10.1080/24701394.2026.2683394","url":null,"abstract":"The complete mitogenome of Hoplia bomiensis Zeng (H. bomiensis) was sequenced and characterized. It was 16,311 bp in length and exhibited conserved gene order, content, and orientation typical of Scarabaeidae. All protein-coding genes (PCGs) began with ATN start codons. The COX1, COX2, and COX3 genes terminated with incomplete stop codon T, while the remaining PCGs ended with TAA or TAG. The non-synonymous (Ka) to synonymous (Ks) substitution ratios for all 13 PCGs were less than 1, suggesting purifying selection. ATP8 displayed the highest Ka/Ks ratio (0.48) and the largest genetic distance compared to the other PCGs. Sliding window analysis identified ND2 as the most variable gene and COX1 as the most conserved. This mitogenome contained 22 tRNA genes, all exhibiting typical cloverleaf structure except trnS1, which lacked the dihydrouracil (DHU) arm. Codon usage was strongly biased toward A/U-ending codons, particularly UUA, AUU, and UUU. Phylogenetic analysis indicated two clades in the family Scarabaeidae. The first clade contained Aphodiinae and Scarabaeinae. Within the second clade, Sericinae diverged first. Melolonthinae was recovered as a non-monophyletic group with three distinct clades, where H. bomiensis formed a highly divergent lineage and exhibited unstable phylogenetic positions between nucleotide- and amino acid-based trees.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"40-52"},"PeriodicalIF":0.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148201537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytb-based identification and phylogenetic placement of red fox (Vulpes vulpes) in Southeastern Anatolia (Türkiye). 安那托利亚东南部红狐（Vulpes Vulpes）的体细胞鉴定和系统发育定位。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-04-01 Epub Date: 2026-06-16 DOI: 10.1080/24701394.2026.2688164

Gül Olgun Karacan, Şafak Bulut

{"title":"Cytb-based identification and phylogenetic placement of red fox (Vulpes vulpes) in Southeastern Anatolia (Türkiye).","authors":"Gül Olgun Karacan, Şafak Bulut","doi":"10.1080/24701394.2026.2688164","DOIUrl":"10.1080/24701394.2026.2688164","url":null,"abstract":"Non-invasive genetic approaches provide reliable tools for species identification, particularly in cases where morphological traits are ambiguous. This study investigated the potential presence of Rüppell's fox (Vulpes rueppellii) in Southeastern Anatolia (Türkiye), prompted by camera trap images showing features that could be attributed to either V. rueppellii or the red fox (V. vulpes). Mitochondrial cytochrome b (Cytb) sequences were obtained from fecal samples and analyzed using Maximum Likelihood phylogenetic methods, complemented by haplotype network analysis. All sequences showed high similarity (99-100%) to V. vulpes and clustered within the V. vulpes reference sequences. No genetic evidence supporting the presence of V. rueppellii was detected. The haplotype network further confirmed the placement of all samples within the Holarctic lineage of V. vulpes. These findings demonstrate that the individuals observed in the study area belong to V. vulpes, despite morphological variation that could suggest otherwise. The results highlight the importance of mitochondrial DNA-based methods for accurate species identification in non-invasive studies, particularly in regions where closely related taxa may occur.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"68-74"},"PeriodicalIF":0.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148260607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the complete mitogenomes of two phytophagous scarab beetles (Coleoptera: Scarabaeidae) and their phylogenetic implications. 两种植食性金龟子（鞘翅目：金龟子科）的全有丝分裂基因组特征及其系统发育意义。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-04-01 Epub Date: 2026-06-05 DOI: 10.1080/24701394.2026.2683396

Xiaoli Liu, Tao Sun, Wenyu Huang, Xiyue Huang, Ruixi Huang, Xueting Hong, Xunhuan Ding, Jiancheng Zang

{"title":"Characterization of the complete mitogenomes of two phytophagous scarab beetles (Coleoptera: Scarabaeidae) and their phylogenetic implications.","authors":"Xiaoli Liu, Tao Sun, Wenyu Huang, Xiyue Huang, Ruixi Huang, Xueting Hong, Xunhuan Ding, Jiancheng Zang","doi":"10.1080/24701394.2026.2683396","DOIUrl":"10.1080/24701394.2026.2683396","url":null,"abstract":"Toxospathius auriventris Bates and Maladera orientalis Motschulsky, are soil-dwelling insects that are mainly distributed in Xizang, China, and considered to be agricultural pests. This study sequenced and characterized the mitogenomes of T. auriventris and M. orientalis, which were 16,837 bp and 16,972 bp in-length. Both mitogenomes exhibited highly conserved gene order of Scarabaeidae. Most protein-coding genes (PCGs) initiated with typical ATN start codon. The incomplete stop codon T was used in COX1, COX2 (only in T. auriventris) and COX3 genes, whereas typical stop codon TAN was used in other PCGs. The Ka/Ks ratios for each PCG among 18 species from subfamily Melolonthinae were less than 1 (0.078 to 0.69). The genetic distances of PCGs indicated ATP8 (0.47) evolved relatively fast, while COX1 (0.23) and ND4L (0.24) were more conserved. The nucleotide diversity analysis (Pi values) across the 13 PCGs in subfamily Melolonthinae indicated that ND2 gene exhibited the greatest variability. While, COX1 and ND5 presented lower diversity. Phylogenetic trees reconstructed via maximum likelihood (ML) and Bayesian inference (BI) methods suggested that the subfamily Melolonthinae is non-monophyletic, in contrast to the monophyletic Cetoniinae, Rutelinae, and Dynastinae. T. auriventris and M. orientalis were clustered in different clades within the subfamily Melolonthinae.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"53-67"},"PeriodicalIF":0.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148165913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complete plastid genome sequence of Vischeria sp. WL1 and comparative analysis of plastid genomes among Vischeria strains. 肉糜菌WL1质体全基因组序列及肉糜菌各菌株质体基因组比较分析。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-04-01 Epub Date: 2026-05-29 DOI: 10.1080/24701394.2026.2677588

Xiaolan Rao, Limei Liu, Xiaolong Yuan, Wensheng Liang, Xiang Gao

引用次数: 0

Characterization of the complete mitochondrial genome of Pheropsophus javanus Dejean, 1825 (Carabidae, Brachininae). javanus Dejean斐洛菲斯线粒体全基因组的鉴定，1825（蜈蚣科，腕足科）。

IF 0.6

Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2026-01-01 Epub Date: 2026-04-19 DOI: 10.1080/24701394.2026.2652302

Yang Yue, Kang Zhang, Reymart Rondez Toñacao, Yu Chen, Laizheng Jiao

{"title":"Characterization of the complete mitochondrial genome of Pheropsophus javanus Dejean, 1825 (Carabidae, Brachininae).","authors":"Yang Yue, Kang Zhang, Reymart Rondez Toñacao, Yu Chen, Laizheng Jiao","doi":"10.1080/24701394.2026.2652302","DOIUrl":"10.1080/24701394.2026.2652302","url":null,"abstract":"The mitochondrial genomes (mitogenomes) of insects are widely used for species identification, phylogenetics, and population genetics. Bombardier beetles are among the most important taxa of natural enemy insects worldwide, playing a crucial role in maintaining ecosystem stability and regulating pest population sizes. In this study, we sequenced and assembled the complete mitogenome of Pheropsophus javanus, the first species within the genus Pheropsophus for which a complete mitogenome has been characterized. The full-length mitogenome is 16,752 bp and has an AT content of 78.48%. The complete mitogenome contained 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a putative control region (CR). Phylogenetic analysis revealed that P. javanus and Brachinus crepitans formed a strongly supported sister clade, highlighting the close evolutionary relationship between the genera Pheropsophus and Brachinus. This study clarifies the phylogenetic position of Pheropsophus within the family Carabidae through phylogenetic analysis. Additionally, the complete mitogenome reported here provides valuable genomic resources for future phylogenetic and taxonomic studies of Pheropsophus species.","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"11-20"},"PeriodicalIF":0.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147719100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0