Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献

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The complete mitochondrial genome and phylogenetic position of Schizothorax argentatus (Cyprinomorpha: Crypriniformes: Cyprinidae). Schizothorax argentatus(鲤形目:鲤形科:Cyprinidae)的完整线粒体基因组和系统发育位置。
Zhengduan Huang, Dilina Rusitanmu, Jie Han
{"title":"The complete mitochondrial genome and phylogenetic position of <i>Schizothorax argentatus</i> (Cyprinomorpha: Crypriniformes: Cyprinidae).","authors":"Zhengduan Huang, Dilina Rusitanmu, Jie Han","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We investigated the vulnerable fish species <i>Schizothorax argentatus</i> Kessler, 1874, using low-coverage whole genome sequencing data. The assembled 16,587 bp mitochondrial genome has a nucleotide composition of A = 29.8%, T = 25.3%, G = 17.9%, and C = 27.0%, containing 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes with a gene arrangement identical to other cofamilial species. Phylogenetic analyses of 71 schizothoracine fishes suggest a close relationship between <i>S. argentatus</i> and <i>Schizothorax eurystomus</i>, sharing a common ancestor with <i>Schizothorax pseudoaksaiensis</i>. Our study supports dividing extant schizothoracine fishes into two tribes, Schizothoracini and Schizopygopsini. The estimated time to most recent common ancestor (<i>t<sub>MRCA</sub></i>) and their distribution imply geological and climatic events during the Miocene around the Qinghai-Tibet Plateau as significant evolutionary drivers explaining the diversification of main clades in schizothoracine fishes.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Opsarius siangi, a new Chedrinae fish species from the Brahmaputra drainage, India. Opsarius siangi,印度布拉马普特拉河流域的一种新的车鱼(Chedrinae)鱼种。
Kavita Kumari, Simanku Borah, Sangeetha M Nair, Vettath Raghavan Suresh
{"title":"<i>Opsarius siangi</i>, a new Chedrinae fish species from the Brahmaputra drainage, India.","authors":"Kavita Kumari, Simanku Borah, Sangeetha M Nair, Vettath Raghavan Suresh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>Opsarius siangi</i> sp. nov., a previously undocumented species, has been identified from Siang River, Pasighat, Arunachal Pradesh, India. This newly described species is distinguished by a suite of unique morphological characteristics, notably including a complete lateral line, consisting of 65-77 scales, 32-39 pre-dorsal scales, 12-15 scales positioned between dorsal fin origin and lateral line, presence of two pairs of barbels, body depth ranging from 18.80% to 27.42% of standard length and a distinct pattern of 8-15 vertical bars adorning the body. A comprehensive genetic analysis was conducted by scrutinizing 78 Cytochrome oxidase I (COI) sequences extracted from Chedrinae fishes, with particular focus on <i>Opsarius</i> and <i>Barilius</i> genera. Phylogenetic analysis revealed that <i>O. siangi</i> sp. nov. occupies a distinctive clade, displaying close affinity with <i>O. shacra</i>. Intraspecific K2P genetic divergence, assessed at 0.02, falls well within established species delineation thresholds, while interspecific divergence in comparison to <i>O. shacra</i> was recorded at 0.112. Complementary species delimitation methodologies, including BIN and bPTP, further underscore taxonomic uniqueness of <i>O. siangi</i> sp. nov., within Chedrinae family. This description enriches our understanding of biodiversity within Siang River ecosystem and underscores the merit of employing multi-pronged approaches in taxonomic investigations.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139673807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association between mitochondrial DNA genotype and sperm motility in humans. 人类线粒体 DNA 基因型与精子活力之间的关系。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2023-01-01 Epub Date: 2024-06-24 DOI: 10.1080/24701394.2024.2361609
Xueyou Song, Xiaoning Hong, Zilong Wang, Fuding Lu, Changze Song, Xinkun Wang, Xiaoyong Zhan, Jiaying Yu, Jiawen Zhai, Jiang Li, Xi Xiang, Xujun Xuan
{"title":"Association between mitochondrial DNA genotype and sperm motility in humans.","authors":"Xueyou Song, Xiaoning Hong, Zilong Wang, Fuding Lu, Changze Song, Xinkun Wang, Xiaoyong Zhan, Jiaying Yu, Jiawen Zhai, Jiang Li, Xi Xiang, Xujun Xuan","doi":"10.1080/24701394.2024.2361609","DOIUrl":"10.1080/24701394.2024.2361609","url":null,"abstract":"<p><p>The relationship between genetic alterations in mitochondrial DNA (mtDNA) and progressive motility (PR) and rapid progressive motility (grade A) of ejaculated human spermatozoa remains unclear. In this study, we explored the association between human mtDNA genotype and sperm PR and grade A by analyzing mtDNA copy number, loci, haplogroup, rearrangement, deletions, and duplications and sperm motility parameters. Human sperm mtDNA copy number, loci and haplogroups were not associated with human sperm motility PR or A grade. However, the cumulative frequency of human sperm mtDNA rearrangements (including deletions and duplications) in participants with high PR and grade A ratio was higher than in participants with low PR and grade A ratio. Additional studies are needed to understand the relationship between mtDNA genotypes, including deletions and duplications, and human sperm motility.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"41-48"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction. 修正。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2023-01-01 Epub Date: 2024-05-01 DOI: 10.1080/24701394.2024.2348847
{"title":"Correction.","authors":"","doi":"10.1080/24701394.2024.2348847","DOIUrl":"https://doi.org/10.1080/24701394.2024.2348847","url":null,"abstract":"","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":"34 1-8","pages":"I"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitogenomic analysis of Rüppell's fox (Vulpes rueppellii) confirms phylogenetic placement within the Palaearctic clade shared with its sister species, the red fox (Vulpes vulpes). 对吕佩尔狐(Vulpes rueppellii)的有丝分裂基因组分析证实了其在系统发育上与姊妹物种赤狐(Vulpes vulpes)同属古北欧支系。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2023-01-01 Epub Date: 2024-04-07 DOI: 10.1080/24701394.2024.2332320
Ali Elsayed Basuony, Mostafa Saleh, Frank Hailer
{"title":"Mitogenomic analysis of Rüppell's fox (<i>Vulpes rueppellii</i>) confirms phylogenetic placement within the Palaearctic clade shared with its sister species, the red fox (<i>Vulpes vulpes</i>).","authors":"Ali Elsayed Basuony, Mostafa Saleh, Frank Hailer","doi":"10.1080/24701394.2024.2332320","DOIUrl":"10.1080/24701394.2024.2332320","url":null,"abstract":"<p><p>The Rüppell's fox (<i>Vulpes rueppellii</i>) inhabits desert regions across North Africa, the Arabian Peninsula and southwestern Asia. Its phylogenetic relationship with other fox species, especially within the phylogeographic context of its sister species, <i>V. vulpes</i>, remain unclear. We here report the sequencing and de-novo assembly of the first annotated mitogenome of <i>V. rueppellii</i>, analysed with data from other foxes (tribe Vulpini, subfamily Caninae). We used four bioinformatic approaches to reconstruct the <i>V. rueppellii</i> mitogenome, obtaining identical sequences except for the incompletely assembled tandem-repeat region within the D-loop. The mitogenome displayed an identical organization, number and length of genes as <i>V. vulpes</i>. We found high support for clustering of both known subclades of <i>V. rueppellii</i> within the Palearctic clade of <i>V. vulpes</i>, rendering the latter species paraphyletic, consistent with previous analyses of shorter mtDNA fragments. More work is needed for a full understanding of the evolutionary drivers and consequences of hybridization in foxes.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"22-28"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140871620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial genome rearrangements and phylogenomics of the Hymenoptera (Insecta) using an expanded taxon sample. 利用扩大的类群样本进行膜翅目(昆虫)线粒体基因组重排和系统发生组学研究。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2023-01-01 Epub Date: 2024-05-16 DOI: 10.1080/24701394.2024.2345663
Nan Song, Shu-Jun Wei, Miaomiao Wang
{"title":"Mitochondrial genome rearrangements and phylogenomics of the Hymenoptera (Insecta) using an expanded taxon sample.","authors":"Nan Song, Shu-Jun Wei, Miaomiao Wang","doi":"10.1080/24701394.2024.2345663","DOIUrl":"10.1080/24701394.2024.2345663","url":null,"abstract":"<p><p>The order Hymenoptera is one of the most species-rich insect orders, with more than 150,000 described extant species. Many hymenopteran insects have very different mitochondrial genome (mitogenome) organizations compared to the putative ancestral organization of insects. In this study, we sequenced 18 mitogenomes of representatives in the order Hymenoptera to increase taxonomic sampling. A total of 475 species were used in phylogenetic analyses, including 18 new mitogenomes and 457 existing mitogenomes. Using a site-heterogeneous model, Bayesian's inference from amino acid data yielded more resolved relationships among Hymenoptera than maximum-likelihood analysis and coalescent-based species analyses. The monophyly of Symphyta was not supported. The Xyeloidea was the earliest branching clade in the Hymenoptera. The Orussoidea was closely related to Apocrita. Within Apocrita, the Parasitoida was non-monophyletic. The monophyly of most Parasitoida superfamilies received strong support. The Proctotrupomorpha clade was supported in Bayesian's analysis. The Apoidea was monophyletic when excluding <i>Ampulex compressa</i> from consideration. The superfamilies Vespoidea and Chrysidoidea were found to be non-monophyletic. Comparisons of mitochondrial gene order revealed a higher frequency of gene rearrangement among lineages with a parasitoid lifestyle, particularly prominent in Chalcidoidea. The degree of gene rearrangement ranked second in specific taxa of Cynipoidea and Ichneumonoidea.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"49-65"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140946645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction. 校正
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2022-01-01 Epub Date: 2022-07-21
{"title":"Correction.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"iii"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40636396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
DNA barcoding and delimitation of critically endangered indigenous and introduced tilapias (pisces cichlidae) of Pangani catchment, Northern Tanzania. 坦桑尼亚北部潘加尼集水区极度濒危的本土和引进罗非鱼(pisces cichlidae)的 DNA 条形码和划界。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2022-01-01 Epub Date: 2024-01-02
Yeremia Japhet Chuhila, Fred Demetrius Chibwana, Jestina Venance Katandukila, Chacha John Mwita
{"title":"DNA barcoding and delimitation of critically endangered indigenous and introduced tilapias (pisces cichlidae) of Pangani catchment, Northern Tanzania.","authors":"Yeremia Japhet Chuhila, Fred Demetrius Chibwana, Jestina Venance Katandukila, Chacha John Mwita","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Pangani catchment of Northern Tanzania harbours the critically endangered endemic tilapias of the genus <i>Oreochromis</i>. The introduction of non-native congenerics and consequent hybridization complicates taxa identification and phylogeny based on morphological systematics. We therefore morphologically and molecularly identified these tilapias and delimited their Molecular Operational Taxonomic Units (MOTUs) based on Cytochrome Oxidase Subunit I (CO1) gene for future management and conservation. A total of 132 indigenous and introduced tilapia specimens were morphologically identified, barcoded using the CO1 gene and delimited by Kimura 2 Parameter distance approaches, Automatic Barcode Gap Discovery (ABGD), Neighbour Joining (NJ) tree and haplotype analysis. Theoverall mean conspecific, congeneric and confamillial genetic distances based on the K2P model were 0.54%, 5.32% and 13.29% respectively. All taxa had a mean K2P distance < 2% and 90% (<i>n</i> = 10), were clearly delimited by the ABGD method. The NJ tree delimited tilapia taxa commensurate to the genetic distances depicted by DNA barcoding. However, DNA barcoding and NJ tree coherently failed to discriminate the morphologically distinct allopatric <i>Oreochromis jipe</i> and <i>Oreochromis hunteri</i> taxa. Moreover, the two methods depicted lack of monophyly in <i>Oreochromis korogwe</i> MOTUs implying that the taxon could consist of at least one MOTU. We conclude that the integration of morphological-based taxonomy and DNA barcoding among ichthyofaunal taxa herein will be invaluable in conservation and management of native tilapias in Pangani basin.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"40-52"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139076141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Complete chloroplast genome of Lamiophlomis rotata: comparative genome analysis and phylogenetic analysis. Lamiophlomis rotata 的完整叶绿体基因组:比较基因组分析和系统发育分析。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2022-01-01 Epub Date: 2024-01-08
Ji Wang, Xing-Xing Mao, Yazhen Ma
{"title":"Complete chloroplast genome of <i>Lamiophlomis rotata</i>: comparative genome analysis and phylogenetic analysis.","authors":"Ji Wang, Xing-Xing Mao, Yazhen Ma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p><i>Lamiophlomis rotata</i>, the only species within the genus <i>Lamiophlomis</i> (family Labiatae), exhibits a broad geographical distribution in elevated highland areas in Qinghai-Tibetan Plateau and possesses significant therapeutic properties. Numerous chemical compositions and putative phylogenetic affiliations of this species have been documented in prior research. Nevertheless, there is a scarcity of accessible publications regarding the genomic data of <i>L. rotata</i>, particularly its chloroplast genome. This dearth of knowledge hampers the comprehensive investigation of its phylogenetic placement within the Labiatae family. In this study, we present a comprehensive analysis of the plastid genome of <i>L. rotata</i>. The plastid genome has a length of 151,837 base pairs (bp) and a GC content of 38.5%. Within this genome, a total of 135 genes were identified, including 90 protein-coding genes, 37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. By employing phylogenetic analysis, the taxonomic position of <i>L. rotata</i> within the family Labiatae is elucidated, highlighting a close relationship between the genus <i>Lamiophlomis</i> and the genus <i>Phlomis</i>. Notably, extensive genetic variations were uncovered between <i>L. rotata</i> and other <i>Phlomis</i> species. This study could provide significant insights for understanding the phylogenetic relationships of taxa within Labiatae.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"29-39"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139378959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Whole mitochondrial genome phylogeny of Drosophilidae. 果蝇科全线粒体基因组系统发育。
Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis Pub Date : 2022-01-01 Epub Date: 2024-01-25
Rob DeSalle, Sara Oppenheim, Patrick M O'Grady
{"title":"Whole mitochondrial genome phylogeny of Drosophilidae.","authors":"Rob DeSalle, Sara Oppenheim, Patrick M O'Grady","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 241 mitochondrial genomes were assembled and annotated from the SRA database to reconstruct a mtDNA genome phylogeny for the genus <i>Drosophila</i>, the family Drosophilidae, and close relatives. The resulting mtDNA genome phylogeny is largely congruent with previous higher-level analyses of <i>Drosophila</i> species with the exception of the relationships between the melanogaster, montium, anannassae, saltans and obscura groups. Although relationships within these species groups are congruent between nuclear and mtDNA studies, the mtDNA genome phylogeny of the groups is different when compared to earlier studies. Monophyly of known species groups within the genus <i>Drosophila</i> are highly supported and, as in previous work, the genera <i>Lordiphosa</i>, <i>Hirtodrosophila</i>, <i>Zaprionus</i> and <i>Scaptomya</i> are all imbedded within the genus <i>Drosophila</i>. Incongruence and partitioned support analyses indicate that DNA sequences are better at resolving the phylogeny than their translated protein sequences. Such analyses also indicate that genes on the minus strand of the circular molecule (Lrrna, Srrna, ND4, ND4L and ND5) provide most of the support for the overall phylogenetic hypothesis.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139547593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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