Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis最新文献

{"title":"Genetic diversity and population structure of giant trevallies (<i>Caranx ignobilis</i> Forsskål, 1775) in the Philippines with implications to management.","authors":"Shenna Kate M Torres, Brian S Santos","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The giant trevallies (<i>Caranx ignobilis</i>) is a globally important fish species that is at risk from overexploitation. In this study, 150 <i>C. ignobilis</i> from six provinces in the Philippines were collected for genetic analyses. For each province, five representative specimens of <i>C. ignobilis</i> were subjected to DNA barcoding and revealed high interspecific K2P distances of 9.58% and 17.29% when compared to other species of <i>Caranx</i> and <i>Carangoides</i>, respectively. On the other hand, all 150 <i>C. ignobilis</i> specimens were subjected to population genetic analysis using the mitochondrial cytochrome b region. In the studied population of <i>C. ignobilis</i>, 33 unique haplotypes were observed, and the population exhibited high haplotype (<i>h</i> = 0.831) and nucleotide (π=0.930%) diversity. Pairwise F_ST values between the six study sites indicated limited genetic differentiation among the studied populations. The limited genetic differentiation may be due to the oceanic currents in the Philippines facilitating larval dispersal as observed in the results of the Lagrangian dispersion model. Data from neutrality tests, mismatch distribution, and Bayesian skyline plot revealed that the population may have undergone demographic expansion. This study provides valuable genetic information on <i>C. ignobilis</i> that can be used for formulating sustainable fishery management strategies.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"10-23"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139076142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"ii"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40624640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA barcoding of Schizothoracinae fishes from the Yarlung Zangbo River in Tibet.","authors":"Chi Zhang, Liying Sui, Xuekai Han","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The nine endemic species of the genus <i>Schizothorax</i> from the Yarlung Zangbo River, Tibet comprise a putative cyprinid species flock. In this study, the effectiveness of the COI DNA barcode for Schizothoracinae species identification was verified by using 45 COI sequences covering nine species in four genera of Schizothoracinae fishes. The average Kimura two parameter (K2P) genetic distances within and among species were 0.13% and 8.57%, respectively. The results revealed that most of the species were clearly discriminated by their estimated genetic distances and monophyletic clustering in a maximum likelihood tree. However, insignificant genetic distances were noticed in two reportedly valid species: <i>Schizothorax molesworthi</i> and <i>S. integrilabiatus</i> (0.1%), and the monophyly of <i>S. macropogon</i> could not be recovered in the schizothoracine group. The fishes of <i>S</i>.<i>curilabiatus</i> living in the lower course of Yalung Zangbo River were clustered together with three species from the upper course, which is inconsistent with the geographical distribution of the populations.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"24-28"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139081134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Signature of climatic differentiation on mitochondrial DNA of <i>Drosophila sturtevanti</i>.","authors":"Samara Videira Zorzato, Amir Yassin, Lilian Madi-Ravazzi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pleistocene climatic changes have played a major role in the evolution of Brazilian Atlantic Forest and South America biodiversity but their impacts on the genetic structure of widely distributed species remain unclear. Here, we investigate mitochondrial DNA (mtDNA) diversity in 21 geographical populations of <i>Drosophila sturtevanti</i>, Nucleotide sequences of the cytochrome C oxidase subunits I and II genes (<i>COI</i> and <i>COII</i>, respectively<i>)</i> from 163 individuals, showed a significant north-south structure, in spite of an overall low level of variation. The haplotypes clustered in three groups that showed strong correlations with geographical and climatic variables, suggesting that local adaptations might have contributed to differentiation within the species. Coalescent-based analyses indicated that the three clusters have differentiated nearly ∼17.000 years ago, suggesting a major role for Pleistocene changes in shaping current day distributions and differentiation of widespread Neotropical species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"153-161"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39778504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic structure and demographic history of endangered <i>Alburnus tarichi</i> (Güldenstädt, 1814) populations from Lake Van basin in Turkey inferred from mtDNA analyses.","authors":"Yılmaz Çiftci, Oğuzhan Eroğlu, Şirin Firidin, Hacı Savaş, Yusuf Bektaş","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genetic diversity, genetic structure, and demographic history of the endemic and endangered cyprinid species <i>Alburnus tarichi</i> based on samples from 17 populations consisting of resident and potamodromous specimens from the Lake Van basin in eastern Turkey were analyzed using two mitochondrial DNA markers. <i>A. tarichi</i> populations in the Lake Van basin are genetically heterogeneous, as indicated by the high haplotype and low nucleotide diversity of 1233 bp of the 16S rRNA marker (44 haplotypes; 70 polymorphic sites, haploid diversity (<i>H_d</i>) = 0.9130, π = 0.0032) and 1140 bp of the cyt b marker (47 haplotypes; 82 polymorphic sites, <i>H_d</i> = 0.9339, π = 0.0057). Clades were separated by average sequence divergences of 1.94% (II vs. III), 1.80% (I vs. III), and 0.66% (I vs. II). Based on these clades, AMOVA analysis revealed that 80.76% of the total variation occurred among populations, 10.74% occurred within populations, and only 8.50% occurred between populations within groups for the concatenated 16S rRNA-cyt b sequences. Pairwise <i>F_ST</i> values varied from 0.0167 to 0.9705 for the concatenated 16S rRNA-cyt b dataset, emphasizing the high genetic variation among populations. The time since the endemic tarek populations split from their last common ancestor has been dated to 5.647 Ma (95% highest posterior density: 4.183-7.011 Ma) in the Messinian Stage. Recent population expansion for tarek populations has been determined by neutrality tests and mismatch distribution analyses. The results of this study provide valuable information on the genetic population structure, conservation, and management of this species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"162-177"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39937743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High genetic diversity detected in the mitochondrial Control Region of the Serra Spanish Mackerel, <i>Scomberomorus brasiliensis</i> (Collette, Russo & Zavala, 1978) along the Brazilian coast.","authors":"Divino Bruno da Cunha, Kely Cristina Piedade Martins, José Nazareno do Santos Júnior, Edith Cibelle de Oliveira Moreira, Iracilda Sampaio, Cleonilde da Conceição Silva Queiroz, Maria Adriana Leite, Agerdânio Andrade de Souza, Carolina Aviz Dos Santos, Marcelo Vallinoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Serra Spanish mackerel, <i>Scomberomorus brasiliensis</i>, is one of the most important fishery resources in the western tropical Atlantic, including northern and eastern Brazil. Despite its economic importance, few genetic markers have been sequenced in this species, and little is known of its population genetics. The present study evaluated the genetic variability of 110 individuals, representing three distinct Brazilian populations (Macapá, Fortaleza and Paranaguá), based on a segment of the mitochondrial Control Region. The sequences revealed high levels of genetic diversity, and suggested marked connectivity among the studied populations. A variable repeat was also found in the 3' portion of the studied Control Region fragment, which may prove useful as a marker in future genetic population studies of <i>S. brasiliensis</i>.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"178-185"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10580391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mitochondrial genome of the endangered Spiny Butterfly Ray <i>Gymnura altavela</i> (Linnaeus 1758) (Myliobatiformes: Gymnuridae) provides insights into cryptic lineages.","authors":"Tabitha Cady, Katherine E Bemis, J Antonio Baeza","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Spiny Butterfly Ray, <i>Gymnura altavela</i>, is found in tropical and temperate coastal waters with a discontinuous distribution: it occurs along the east coast of the United States of America, Brazil, and Uruguay in the western Atlantic Ocean, from Portugal to Angola in the eastern Atlantic, and also in the Mediterranean Sea. Globally, <i>Gymnura altavela</i> is considered endangered by the International Union for Conservation of Nature (IUCN). Our study analyzed the complete mitochondrial genome of <i>G. altavela</i> sequenced from an individual captured in the western Atlantic and compared it with a mitochondrial genome from a conspecific collected in the Mediterranean to explore if <i>G. altavela</i> comprises cryptic species. The newly assembled mitochondrial genome of <i>G. altavela</i> is 19,361bp in length and has 13 protein-coding genes (PCGs), two ribosomal RNA genes (<i>12s</i> ribosomal RNA and <i>16s</i> ribosomal RNA), 22 transfer RNA (tRNA) genes, and a 3,659 bp control region. The gene order is identical to that reported for the specimen collected in the Mediterranean and that of the congeneric Long-tailed Butterfly Ray, <i>G. poecilura</i>. A phylomitogenomic analysis based on translated PCGs supported the monophyly of the genus <i>Gymnura</i> and indicated that genetic dissimilarity between <i>G. altavela</i> from the western Atlantic Ocean and the Mediterranean Sea was greater than that calculated among congeneric species belonging to the genera <i>Mobula</i> and <i>Neotrygon</i>, similar to that calculated among congeneric stingrays in the genus <i>Hemitrygon</i>, but lower than that calculated among congeneric species in the genera <i>Pateobatis</i> and <i>Urogymnus</i>. Overall, our comparisons suggest that <i>G. altavela</i> comprises cryptic species or at least indicates considerable genetic structure between populations in the western Atlantic and Mediterranean. We present these findings in a phylomitogenomic analysis of other Myliobatiformes and Rhinopristiformes. Additional phylogeographic and taxonomic studies of <i>G. altavela</i> are needed to support conservation efforts for this endangered ray that may comprise cryptic evolutionary units.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"186-194"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10153976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and comparison of the mitochondrial genomes of the sea star <i>Asterias amurensis</i> (Echinodermata: Asteroidea) in East Asia.","authors":"Yanzhen Du, Kangkang Han, Xiaoqi Zeng, Gang Ni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The starfish <i>Asterias amurensis</i> is a marine pest native to the northwestern Pacific and has successfully invaded southern Australia. <i>Asterias amurensis</i> have caused substantial environmental and economic impacts in both native and non-native regions. However, little information is available about the genetic features of its native populations, especially for those in North China. Here we sequenced the complete mitochondrial genomes of five individuals from different locations in China and compared their characteristics with three mitogenomes available from Japan. Multiple analyses and comparisons revealed little difference in the gene composition, gene order, codon usage, and nucleotide content among the eight mitogenomes. However, intraspecific phylogenetic reconstruction unveiled two divergent lineages between specimens from North China plus Ushimado (Seto Inland Sea, Japan) and northern Japan (Asamushi and Onagawa). This conclusion was backed by an analysis of pairwise genetic distances, which showed that individuals from different lineages had relatively higher values (all above 2%). Based on knowledge of paleoenvironmental and tectonic activity in the northwestern Pacific, the two lineages might have originated during the Early Pliocene due to the isolation of the East China Sea from the Japan Sea/East Sea during that time, while the present-day distribution of these lineages have likely been influenced by the ocean current system.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"212-219"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71429941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity, population structure, and demographic history of the chilhuil sea catfish <i>Bagre panamensis</i> in Northwestern Mexico inferred from mitochondrial DNA.","authors":"Karla Isela Arroyo-Zúñiga, Jasmín Granados-Amores, Deivis Samuel Palacios-Salgado, Viridiana Peraza-Gómez, Fausto Valenzuela-Quiñonez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The chilhuil sea catfish (<i>Bagre panamensis</i>) is an ecologically relevant species contributing to the structure, organization, and functioning of the ecosystems it inhabits. Also, it is an important artisanal fishery resource in the Mexican Pacific coast. This study aimed to determine the genetic diversity, population structure, and historical demography of <i>B. panamensis</i> in the Mexican Pacific. The mitochondrial DNA was amplified from two distinct regions, r16S and COI, resulting in an 1142 bp of the concatenated genes. Low genetic diversity levels were detected for r16S (<i>H</i> = 12; <i>h =</i> 0.131<i>; π</i> = <i>0.0003</i>) and high genetic diversity levels for COI (<i>H</i> = 57; <i>h =</i> 0.9128; <i>π = 0.0039</i>) and the concatenated gene fragments (<i>H</i> = 62; <i>h =</i> 0.9307; <i>π = 0.0023</i>). Population structure analysis indicated 'panmixia' for <i>B. panamensis</i> along the Mexican Pacific. Furthermore, historical demographic analysis (Tajima's <i>D</i>, Fu's <i>F</i>s, mismatch distribution, and Bayesian Skyline plot analyses) supported a population expansion scenario for the studied species.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"195-201"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating food web interactions among microcrustaceans and insect in a tropical shallow lake using DNA-based protocol.","authors":"Andrés R Domingos, Abner Carvalho-Batista, Rafael Robles, Marlene S Arcifa, Fernando L Mantelatto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We developed species-specific primers of five microcrustacean preys, <i>Ceriodaphnia richardi</i>, <i>Diaphanosoma</i> cf<i>. brevireme</i>, <i>Daphnia gessneri, Simocephalus serrulatus, Thermocyclops decipiens</i> and <i>Mesocyclops</i> sp., to analyze food-web interactions involving their two insect predators <i>Rheumatobates crassifemur</i> and <i>Martarega uruguayensis</i> distributed in a tropical shallow lake. We designed internal primers of the COI gene (177-282 bp), and tested them, by means of PCR, for specificity and sensitivity. In our tests for specificity, all primers successfully amplified the DNA target but were species-specific failing to amplify the biomarker from any of the other species tested, even in a mixed DNA sample, including predators' DNA. In tests for sensitivity, primers successfully amplified zooplankton biomarkers from low concentration of DNA extractions and also from digestive tract of predators, even after many hours of ingestion. This technique provides a framework as an efficient tool for evaluation of food-web research in natural aquatic environments, where it is impossible to observe if predation occurs. Furthermore, this technique provides an effective solution for the identification of zooplankton species from the predator's digestive tract, where morphological identification alone is sometimes difficult because predators do not consume the prey but feeds using extra-oral digestion, such is the case of heteropterans.</p>","PeriodicalId":74204,"journal":{"name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","volume":" ","pages":"202-211"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71429942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}