Microscopy (Oxford, England)最新文献_第8页

In-situ heating-and-electron tomography for materials research: from 3D (in-situ 2D) to 4D (in-situ 3D). 用于材料研究的原位加热-电子断层扫描：从三维（原位二维）到四维（原位三维）。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfae008

Satoshi Hata, Shiro Ihara, Hikaru Saito, Mitsuhiro Murayama

{"title":"In-situ heating-and-electron tomography for materials research: from 3D (in-situ 2D) to 4D (in-situ 3D).","authors":"Satoshi Hata, Shiro Ihara, Hikaru Saito, Mitsuhiro Murayama","doi":"10.1093/jmicro/dfae008","DOIUrl":"10.1093/jmicro/dfae008","url":null,"abstract":"In-situ observation has expanded the application of transmission electron microscopy (TEM) and has made a significant contribution to materials research and development for energy, biomedical, quantum, etc. Recent technological developments related to in-situ TEM have empowered the incorporation of three-dimensional observation, which was previously considered incompatible. In this review article, we take up heating as the most commonly used external stimulus for in-situ TEM observation and overview recent in-situ TEM studies. Then, we focus on the electron tomography (ET) and in-situ heating combined observation by introducing the authors' recent research as an example. Assuming that in-situ heating observation is expanded from two dimensions to three dimensions using a conventional TEM apparatus and a commercially available in-situ heating specimen holder, the following in-situ heating-and-ET observation procedure is proposed: (i) use a rapid heating-and-cooling function of a micro-electro-mechanical system holder; (ii) heat and cool the specimen intermittently and (iii) acquire a tilt-series dataset when the specimen heating is stopped. This procedure is not too technically challenging and can have a wide range of applications. Essential technical points for a successful 4D (space and time) observation will be discussed through reviewing the authors' example application.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"133-144"},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11000667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of electron microscopic observations to electrochemistry in liquid electrolytes for batteries. 电子显微镜观察在电池液态电解质电化学中的应用。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfad044

Kaname Yoshida, Yuki Sasaki, Akihide Kuwabara, Yuichi Ikuhara

引用次数: 0

Cinematographic study of stochastic chemical events at atomic resolution. 原子分辨率下随机化学事件的电影摄影研究。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfad052

Koji Harano, Takayuki Nakamuro, Eiichi Nakamura

引用次数: 0

Perspective and prospects of in situ transmission/scanning transmission electron microscopy. 原位透射/扫描透射电镜技术的展望与展望。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfad057

Renu Sharma, Wei-Chang David Yang

{"title":"Perspective and prospects of in situ transmission/scanning transmission electron microscopy.","authors":"Renu Sharma, Wei-Chang David Yang","doi":"10.1093/jmicro/dfad057","DOIUrl":"10.1093/jmicro/dfad057","url":null,"abstract":"In situ transmission/scanning transmission electron microscopy (TEM/STEM) measurements have taken a central stage for establishing structure-chemistry-property relationship over the past couple of decades. The challenges for realizing 'a lab-in-gap', i.e. gap between the objective lens pole pieces, or 'a lab-on-chip', to be used to carry out experiments are being met through continuous instrumental developments. Commercially available TEM columns and sample holder, that have been modified for in situ experimentation, have contributed to uncover structural and chemical changes occurring in the sample when subjected to external stimulus such as temperature, pressure, radiation (photon, ions and electrons), environment (gas, liquid and magnetic or electrical field) or a combination thereof. Whereas atomic resolution images and spectroscopy data are being collected routinely using TEM/STEM, temporal resolution is limited to millisecond. On the other hand, better than femtosecond temporal resolution can be achieved using an ultrafast electron microscopy or dynamic TEM, but the spatial resolution is limited to sub-nanometers. In either case, in situ experiments generate large datasets that need to be transferred, stored and analyzed. The advent of artificial intelligence, especially machine learning platforms, is proving crucial to deal with this big data problem. Further developments are still needed in order to fully exploit our capability to understand, measure and control chemical and/or physical processes. We present the current state of instrumental and computational capabilities and discuss future possibilities.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"79-100"},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depth sectioning using environmental and atomic-resolution STEM. 利用环境和原子分辨率 STEM 进行深度剖析。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfae005

Masaki Takeguchi, Ayako Hashimoto, Kazutaka Mitsuishi

引用次数: 0

In-situ TEM study from the perspective of holders. 持子视角下的原位透射电镜研究。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfad055

Toshie Yaguchi, Mia L San Gabriel, Ayako Hashimoto, Jane Y Howe

{"title":"In-situ TEM study from the perspective of holders.","authors":"Toshie Yaguchi, Mia L San Gabriel, Ayako Hashimoto, Jane Y Howe","doi":"10.1093/jmicro/dfad055","DOIUrl":"10.1093/jmicro/dfad055","url":null,"abstract":"During the in situ transmission electron microscopy (TEM) observations, the diverse functionalities of different specimen holders play a crucial role. We hereby provide a comprehensive overview of the main types of holders, associated technologies and case studies pertaining to the widely employed heating and gas heating methods, from their initial developments to the latest advancement. In addition to the conventional approaches, we also discuss the emergence of holders that incorporate a micro-electro-mechanical system (MEMS) chip for in situ observations. The MEMS technology offers a multitude of functions within a single chip, thereby enhancing the capabilities and versatility of the holders. MEMS chips have been utilized in environmental-cell designs, enabling customized fabrication of diverse shapes. This innovation has facilitated their application in conducting in situ observations within gas and liquid environments, particularly in the investigation of catalytic and battery reactions. We summarize recent noteworthy studies conducted using in situ liquid TEM. These studies highlight significant advancements and provide valuable insights into the utilization of MEMS chips in environmental-cells, as well as the expanding capabilities of in situ liquid TEM in various research domains.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"117-132"},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138178185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-situ shearing process observation system for soft materials via transmission electron microscopy. 通过透射电子显微镜观察软材料原位剪切过程的系统。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfad045

Tomohiro Miyata, Hsiao-Fang Wang, Daisuke Watanabe, Yoshiaki Kawagoe, Tomonaga Okabe, Hiroshi Jinnai

{"title":"In-situ shearing process observation system for soft materials via transmission electron microscopy.","authors":"Tomohiro Miyata, Hsiao-Fang Wang, Daisuke Watanabe, Yoshiaki Kawagoe, Tomonaga Okabe, Hiroshi Jinnai","doi":"10.1093/jmicro/dfad045","DOIUrl":"10.1093/jmicro/dfad045","url":null,"abstract":"We developed an in-situ shear test system suitable for transmission electron microscopy (TEM) observations, which enabled us to examine the shear deformation behaviours inside soft materials at nanoscale resolutions. This study was conducted on a nanoparticle-filled rubber to investigate its nanoscale deformation behaviour under a large shear strain. First, the shear deformation process of a large area in the specimen was accurately examined and proven to exhibit an almost perfect simple shear. At the nanoscale, voids grew along the maximum principal strain during shear deformation. In addition, the nanoscale regions with rubber and silica aggregates exhibited deformation behaviours similar to the global shear deformation of the specimen. Although the silica aggregates exhibited displacement along the shearing directions, rotational motions were also observed owing to the torque generated by the local shear stress. This in-situ shear deformation system for TEM enabled us to understand the nanoscale origins of the mechanical properties of soft materials, particularly polymer composites. Graphical Abstract.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"208-214"},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10223834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ microscopy techniques for understanding Li plating and stripping in solid-state batteries. 了解固态电池中锂电镀和剥离的原位显微镜技术。

Microscopy (Oxford, England) Pub Date : 2024-04-08 DOI: 10.1093/jmicro/dfad058

Munekazu Motoyama

引用次数: 0

Bioimaging of glucose analogs labeled at the C-1 or C-2 position with a fluorescent dansylamino group. 在 C-1 或 C-2 位置用荧光丹酰氨基标记的葡萄糖类似物的生物成像。

Microscopy (Oxford, England) Pub Date : 2024-02-07 DOI: 10.1093/jmicro/dfad036

Mio Yanagida, Hirofumi Nakano, Hironori Ueno

{"title":"Bioimaging of glucose analogs labeled at the C-1 or C-2 position with a fluorescent dansylamino group.","authors":"Mio Yanagida, Hirofumi Nakano, Hironori Ueno","doi":"10.1093/jmicro/dfad036","DOIUrl":"10.1093/jmicro/dfad036","url":null,"abstract":"Glucose is the most important energy source in all organisms; however, our understanding of the pathways and mechanisms underlying glucose transportation and localization in living cells is incomplete. Here, we prepared two glucose analogs labeled with a dansylamino group at the C-1 (1-Dansyl) or C-2 (2-Dansyl) position; the dansyl group is a highly fluorescent moiety that is characterized by a large Stokes shift between its excitation and emission wavelengths. We then examined the cytotoxicity of the two glucose analogs in mammalian fibroblast cells and in the ciliated protozoan Tetrahymena thermophila. In both cell types, 2-Dansyl had no negative effects on cell growth. The specificity of cellular uptake of glucose analogs was confirmed using an inhibitor of glucose transporter in NIH3T3 cells. In NIH3T3 cells and T. thermophila, fluorescence microscopy revealed that the glucose analogs localized throughout the cytoplasm, but especially at the periphery of the nucleus. In T. thermophila, we also found that swimming speed was comparable in media containing non-labeled glucose or one of the glucose analogs, which provided more evidence not only that the analogs were not cytotoxic in these cells but also that the analogs had no negative effect on the ciliary motion. Together, the present results suggest that the glucose analogs have low toxicity and will be useful for bioimaging of glucose-related systems.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10137757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and electronic characterization of fluorine-doped La0.5Sr0.5CoO3-δ using electron energy-loss spectroscopy. 利用电子能损耗谱分析掺氟 La0.5Sr0.5CoO3-δ 的结构和电子特性。

Microscopy (Oxford, England) Pub Date : 2024-02-07 DOI: 10.1093/jmicro/dfad031

Ryotaro Aso, Takuya Katsumata, Takashi Nakamura, Yusuke Watase, Koji Amezawa, Yasukazu Murakami

{"title":"Structural and electronic characterization of fluorine-doped La0.5Sr0.5CoO3-δ using electron energy-loss spectroscopy.","authors":"Ryotaro Aso, Takuya Katsumata, Takashi Nakamura, Yusuke Watase, Koji Amezawa, Yasukazu Murakami","doi":"10.1093/jmicro/dfad031","DOIUrl":"10.1093/jmicro/dfad031","url":null,"abstract":"Perovskite oxides, ABO3, are potential catalysts for the oxygen evolution reaction, which is important in the production of hydrogen as a sustainable energy resource. Optimizing the chemical composition of such oxides by substitution or doping with additional elements is an effective approach to improving the activity of such catalysts. Here, we characterized the crystal and electronic structures of fluorine-doped La0.5Sr0.5CoO3-δ particles using scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy (EELS). High-resolution STEM imaging demonstrated the formation of a disordered surface phase caused by fluorine doping. In addition, spatially resolved EELS data showed that fluorine anions were introduced into the interiors of the particles and that Co ions near the surfaces were slightly reduced by fluorine doping in conjunction with the loss of oxygen ions. Peak fitting of energy-loss near-edge structure data demonstrated an unexpected nanostructure in the vicinity of the surface. An EELS characterization comprising elemental mapping together with an energy-loss near-edge structure analysis indicated that this nanostructure could not be assigned to Co-based materials but rather to the solid electrolyte BaF2. Complementary structural and electronic characterizations using STEM and EELS as demonstrated herein evidently have the potential to play an increasingly important role in elucidating the nanostructures of functional materials.","PeriodicalId":74193,"journal":{"name":"Microscopy (Oxford, England)","volume":" ","pages":"22-30"},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9528067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0