American Journal of Biochemistry and Biotechnology最新文献

{"title":"Assessment of Genetic Diversity among Elite Breeding Lines of Arabica Coffee (Coffea arabica L.) in Ethiopia using Simple Sequence Repeats Markers","authors":"T. Benti, E. Gebre, K. Tesfaye, G. Berecha, P. Lashermes, M. Kyalo, N. Yao","doi":"10.3844/ajbbsp.2022.394.404","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.394.404","url":null,"abstract":": Despite being the center of origin and genetic diversity, there has been limited application of molecular markers in arabica coffee breeding in Ethiopia. In this study, the extent of genetic diversity and relationships of 48 elite breeding lines of arabica coffee from different geographical origins were evaluated using 14 SSR markers. The SSR markers amplified 105 alleles of which 104 are polymorphic with an average of 7.4 polymorphic alleles per locus. The average percent of polymorphism and Polymorphic Information Content (PIC) of the SSR markers were 98.81 and 0.79, respectively. The average genetic similarity coefficient among all possible pairs was 0.37 and ranged from 0.39 to 0.46 within the same geographical origin. The Unweighted Pair Group Method Using Arithmetic averages (UPGMA) cluster analysis placed the studied breeding lines into seven clusters with Jaccard’s similarity coefficient of <0.44. The results of this study demonstrated the powerfulness of the SSR markers and the presence of a high level of genetic diversity and wide genetic dissimilarity within and among elite breeding lines from the same and different geographical origins. The observed diversity could be utilized in the breeding program to improve productivity and maintain the typical quality profile of origin-based landrace coffees. The SSR markers could be used in future genetic analysis of arabica coffee germplasm to increase the efficiency of the breeding program in Ethiopia. The studied breeding lines could also be conserved and managed as an important source of genetic variability to be used in the future coffee improvement program.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80686178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorophyll and Protein Changes Observed Post Co-Cultivation of Explants with Agrobacterium Tumefaciens in Soybean (Glycine max L. Merr.)","authors":"P. Mangena","doi":"10.3844/ajbbsp.2022.456.464","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.456.464","url":null,"abstract":": Although, Agrobacterium -mediated genetic transformation represents a major milestone in agricultural biotechnology for crop improvement against biotic and abiotic stresses. Further insights on the interactions between this bacterium and explants required for in vitro plant regeneration remain scant. This study investigated the changes in chlorophyll content and protein profiles of cotyledonary node explants co-cultivated with A. tumefaciens in soybean. Soybean seeds were germinated on MS medium supplemented with 4.0 mgL -1 6-BA to develop seedlings used for explant preparation and infection with Agrobacterium . The results indicated that 6-BA decelerated germination, inhibiting normal seedling development and resulting in stout seedlings with stunted epicotyls and reduced primary roots without lateral roots. According to these results, Agrobacterium co-cultured explants gave the mean chlorophyll content (mg g -1 ) ranging between 13.2±0.892 to 18.7±1.478 in all cultivars, compared to the controls at 14.2±0.113 to 50.5±18.04. Protein lysates derived from these explants contained a combination of high and low molecular weight proteins, in which expressed predominant protein concentration ranged between 10-100 kDa for control explants and 10-120+ kDa for Agrobacterium infected and co-cultured cotyledonary tissues. This study revealed changes in chlorophyll and protein profile post-co-cultivation of explants with Agrobacterium , thus, providing further insights on the role of this bacterium on explant response for subsequent in vitro genetic transformation in soybean","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75561772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Anti-Inflammatory Potential of Turmimax® an in vivo and in vitro Study","authors":"Firoz Hussain H M., N. S., S. Thimmegowda, Neethu Benny, R. Y.","doi":"10.3844/ajbbsp.2022.386.393","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.386.393","url":null,"abstract":": The main objective of this study was to investigate the in vivo and in vitro anti-inflammatory effects of the Turmimax ® bio-enhanced turmeric formulation. By comparing the relative fluorescence intensities of the interleukins IL-8 (Interleukin-8) and IL-12 (Interleukin-12) cytokines in stimulated murine Raw 264.7 macrophages, Turmimax ® was evaluated for its anti-inflammatory in vitro efficacy. Turmimax ® was administered orally in two doses, 100 and 200 mg/kg, to assess its in vivo anti-inflammatory potential in ear edema induced by croton oil in mice and paw edema induced by histamine in albino rats. Diclofenac sodium 10 mg/kg p.o. is the standard drug. The results demonstrated that Turmimax ® significantly and dose-dependently decreased the production of the inflammatory mediator’s interleukin-8 and IL-12. According to the current investigation results, Turmimax ® has shown strong anti-inflammatory effects.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84120526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity and Apoptosis-Induction Potential of Fraction F3 from Fruits of Bersama engleriana (Melanthaceae) against Human Cervical Cancer Cells","authors":"M. S. Majoumouo, M. Tincho, Thureyah Morris, D. Hiss, F. Boyom","doi":"10.3844/ajbbsp.2022.428.436","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.428.436","url":null,"abstract":"","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89612828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research and Development for the Hurdle Avoidance During Transplanting Manipulation Based on Kinect Vision Processing","authors":"Kaikang Chen, Yanwei Yuan, Bo Zhao, Liming Zhou, R. Li, K. Niu, Ming-Zhong Xu, Chang-Hui Wang, na Han, Xinhai Jin, Yongjun Zheng","doi":"10.3844/ajbbsp.2022.370.377","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.370.377","url":null,"abstract":": To avoid damage to plants during the transplanting process, a transplanting method based on the combination of the Kinect machine visual system and the low-damage transplanting end effector was proposed to reduce the damage to the stems and leaves from transplanting end effectors during the transplanting process. The inclined manipulator was designed can avoid the characteristics of the seedling itself during the transplanting process to avoid obstacles. Under the experimental condition, the oblique transplanting manipulator assisted by the Kinect vision processing system achieved 4.70% leaf damage, 16.70% stem bending, 83.45% substrate integrity, and the transplanting quality parameters were 87.36%. The completion time of a single transplant was 8.32±0.4 s, the results proved that the low-damage transplanting manipulator based on the machine vision system can effectively reduce the damage rate of the stems and leaves. This obstacle avoidance transplant method can be extended to seedlings planted in 50 plugs. Research and development for hurdle avoidance during transplanting manipulation based on Kinect vision processing can provide theoretical support for future obstacle avoidance transplantation.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91264409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequencies of the C1236T, G2677T/A, and C3435T Polymorphisms of the MDR1 Gene and their Haplotypes in Uzbekistan","authors":"S. Nurmatova, A. Abdurakhimov, O. Charishnikova, S. Turdikulova, D. Dalimova","doi":"10.3844/ajbbsp.2022.437.446","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.437.446","url":null,"abstract":"","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"33 Suppl 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79786738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profiles and Anti-Diabetic Potential of the Extract of Pueraria lobata Compound Teabags: Preparation and Optimization Infusion Conditions","authors":"Qing Liu, Wen-Quan Shi, Fu Xue, Chengbo Wu, H. Mohamed, Min Li, Yuanda Song","doi":"10.3844/ajbbsp.2022.405.416","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.405.416","url":null,"abstract":": A Pueraria lobata root compound teabag using Pueraria lobate root, Stigma maydis, and Prunus mume as the main material was prepared in this study, and the brewing technology was optimized using Response Surface Methodology (RSM). The sensory evaluation, total polysaccharide, and phenolic compound content were used as evaluation indicators and the effect of the compound ratio of raw material was studied. As a result, the compound ratio of Pueraria lobate root, Prunus mume , and Stigma maydis was determined to be 2:2:1 ( m:m:m ). To obtain the best brewing conditions for high inhibition rates of α-amylase and α-glucosidase, the teabag brewing process was optimized using RSM equipped with a 3-factor-3-level Box-Behnken (BBD) experimental design. Three independent variables were assigned to brewing water temperature (80-100℃), amount of water addition (150-200 mL), and soaking time (3-9 min) to design an empirical model best fit in the inhibition rates of α-amylase and α-glucosidase. The range of values for each variable was based on the previous one-factor experiment (results not shown). The optimal brewing process for the preparation of the Pueraria lobata root compound tea was a brewing water temperature of 90.8℃, an amount of water addition of 155 mL, and a soaking time of 5.9 min. The inhibition rate of α-amylase and α-glucosidase could reach 45.09±1.13 and 60.52±2.47%, respectively. These findings suggested that a Pueraria lobata root compound teabag was established, with a unique aroma, a slightly bitter and sweet taste, high polysaccharides and phenolic content, and excellent in vitro hypoglycemic activity.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82991066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Quality Analysis of Pineapple Red Orange Fresh Beer","authors":"Juan Yang, Biaoshi Wang, Xiaojun Hu, Zihao He, Daxiang Li, Tao Cheng","doi":"10.3844/ajbbsp.2022.447.455","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.447.455","url":null,"abstract":"","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81099086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Foliar Applications Mulberry Leaf Enzyme Solution Fertilizer on Growth and Quality of Hydroponic Chinese Cabbage","authors":"Li Yang, F. Yuting, Du Qingjie, Xiao Huaijuan, Wang Jiqing, Li Juanqi","doi":"10.3844/ajbbsp.2022.495.507","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.495.507","url":null,"abstract":"","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83683642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Bacillus Subtilis as Biological Fertilizer by Biotransformation of Corn Steep Liquor","authors":"Xiaojie Ren, Baoyue Liu, Zhuangzhuang He, Xiaolong Wang, Yubin Zhao, M. Jolicoeur, Xinhe Zhao","doi":"10.3844/ajbbsp.2022.358.369","DOIUrl":"https://doi.org/10.3844/ajbbsp.2022.358.369","url":null,"abstract":": Corn steep liquor is a kind of intractable organic waste liquid produced during the processing of corn starch. But it is rich in nutrients, soluble protein, and amino acids and it could be used as a nitrogen source in the fermentation industry. Many starch processing enterprises cannot directly utilize corn steep liquor and choose to discharge it directly, thus causing environmental pollution. Studies have shown that high ammonia nitrogen in corn steep liquor can promote the increase of microbial biomass. B. subtilis is the dominant strain of microbial flora in corn steep liquor, it is a classic strain in the research and industrial production of biological bacterial fertilizer. In this study, the dominant B. subtilis 3301, which is suitable for high-density cultivation in corn steep liquor, was screened first. Then the optimal fermentation parameters, like corn steep liquor concentration, pH, and inoculation amount of the bacteria were analyzed by a three-factor and three-level Response Surface (RSM) analysis experiment. The highest cell density was obtained when the concentration of corn steep liquor is 145 g·L -1 , pH is 7 and the inoculation amount is 8. Then the dynamic changes of B. subtilis cultured in corn pulp and beef extract peptone medium (NB) were compared under response surface optimization fermentation conditions. The results showed that B. subtilis cultured in corn pulp medium was significantly higher than in NB medium. Finally, the fermentation effect of fed-batch glucose on the culture of B. subtilis in corn steep liquor was investigated and the results showed that when feeding with glucose, the number of viable bacteria can reach 1.75 × 10 10 CFUmL -1 , which is significantly higher than the number of viable bacteria cultured without feeding glucose. All of the above results show that the optimized medium using cheap corn-steep liquor shows a good production level. The research results provide a theoretical reference for further improving the biomass of biological bacterial fertilizer and at the same time provide a practical reference for the industrial production of biological bacterial fertilizer to reduce the cost of raw materials, alleviate the pollution of corn steep liquor to the environment and promote the further development of the crop processing industry.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74561086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}