American Journal of Biochemistry and Biotechnology最新文献_第10页

Comparative Study on Microbial Communities of Soil, Grape Must and Wine Fermentation of Cabernet Sauvignon Inoculated with three Commercial Yeast of Saccharomyces cerevisiae 三种酿酒酵母菌接种赤霞珠发酵过程中土壤、葡萄和葡萄酒微生物群落的比较研究

American Journal of Biochemistry and Biotechnology Pub Date : 2021-04-01 DOI: 10.3844/ajbbsp.2021.448.464

Rongbin Li, Xuehong Shi, Yaqiong Liu, Haoran Wang, Xiaofa Fu, Huanxiang Wang, Jianlou Mu, Jie Wang

{"title":"Comparative Study on Microbial Communities of Soil, Grape Must and Wine Fermentation of Cabernet Sauvignon Inoculated with three Commercial Yeast of Saccharomyces cerevisiae","authors":"Rongbin Li, Xuehong Shi, Yaqiong Liu, Haoran Wang, Xiaofa Fu, Huanxiang Wang, Jianlou Mu, Jie Wang","doi":"10.3844/ajbbsp.2021.448.464","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.448.464","url":null,"abstract":"Corresponding Author: Jie Wang College of Food Science and Technology, Hebei Agricultural University, Hebei, 071000, China Email: wangjie@hebau.edu.cn Abstract: This study examined the microbial diversity in soil, grape must and wine fermentation of Cabernet Sauvignon inoculated with three different starters (S01: Xinjiang Saccharomyces cerevisiae CEC01; SCA: Ningxia Saccharomyces cerevisiae CECA; S96: Saccharomyces cerevisiae AWRI 796) using a high-throughput sequencing technique. The results showed that 227 bacterial genera and 20 fungi genera were shared in all samples, consisting of Saccharomyces, Filobasidium, Colletotrichum, Alternaria by Venn diagram analysis. Principle component analysis showed the microbiota structures between S01, SCA and S96 fermentation were similar, the major bacterial genera were Pseudomonas, unclassified_f_Enterobacteriaceae and Lactobacillus, whereas the major fungi genus was Saccharomyces. The biomarkers of bacterial genera in S01, SCA and S96 groups were detected using LEfSe analysis, in which Komagataeibacter, Micromonospora, Streptomyces, Brevibacterium and Agromyces were core microorganisms in the S01 group, SCA fermentation increased the relative abundance of Lactobacillus and Oenococcus, family Ruminococcaceae was dominant in the S96 group. The distinctions in fungi communities between S01, SCA and S96 group were not observed during the fermentation. Understanding of microbial diversity could aid to promote the formation of regional characteristics and the development of high-quality wines through the management of existing microorganisms in future.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90845357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1 利用金氧化钇SW1从玉米浸泡液中生产功能性脂肪酸DHA

American Journal of Biochemistry and Biotechnology Pub Date : 2021-04-01 DOI: 10.3844/ajbbsp.2021.373.383

Xiaojie Ren, Qinwen Yan, Baoyue Liu, Yu Du, Xinhe Zhao, Yuanda Song

{"title":"Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1","authors":"Xiaojie Ren, Qinwen Yan, Baoyue Liu, Yu Du, Xinhe Zhao, Yuanda Song","doi":"10.3844/ajbbsp.2021.373.383","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.373.383","url":null,"abstract":"Corresponding Author: Xinhe Zhao School of Agriculture Engineering and Food Science, Colin Ratledge Center for Microbial Lipids, China Email: zhaoxinhe2018@163.com Abstract: Thraustochytrium has a relatively high lipid content in the cell and its lipid content can account up to 70% of the dry cell weight. It is an important raw material for the third-generation biodiesel production and the production of functional fatty acid Docosahexaenoic Acid (DHA). However, the high cost of traditional fermentation medium has hindered the industrial production of lipid. Therefore, searching for raw materials that can be used by thraustochytrid with a wide range of sources and low prices has become a research hotspot in the production of lipids by thraustochytrid. In this study, corn steep liquor was applied for lipid production in thraustochytrid Aurantiochytrium SW1, various factors of corn medium were optimized by using Response Surface Methodology (RSM), including glucose concentration, Rotation Speed, Kinetin concentration and dilution rate of corn steep liquor. The optimal conditions were finally obtained as 72.37 g/L glucose, 2.28 mg/L kinetin, 270 rpm of rotation speed and 15 times of corn pulp dilution. The biomass, lipid and DHA yields obtained under the optimized conditions were 13.5, 7.9 and 42% of total fatty acids, respectively. Comparison with the traditional medium, the biomass, lipid and DHA production were promoted by 14.8, 31.6 and 31.3% respectively. The optimized medium using cheap corn steep liquor showed a better production level, which proved the feasibility of culturing Aurantiochytrium SW1 with cheap raw materials.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80506958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review on Effects of Bisphenol a on Reproductive, Endocrine Systems and its Influencing Mechanism 双酚A对生殖、内分泌系统的影响及其影响机制研究进展

American Journal of Biochemistry and Biotechnology Pub Date : 2021-04-01 DOI: 10.3844/ajbbsp.2021.408.422

Ganghui Ye, Liting Zheng, C. Meng, Yuefeng Dong, Hongyan Xu, Qinglu Wang

引用次数: 0

Identification of Microbe-Drug Association based on Weighted Profile and Collaborative Matrix Factorization 基于加权轮廓和协同矩阵分解的微生物-药物关联鉴定

American Journal of Biochemistry and Biotechnology Pub Date : 2021-04-01 DOI: 10.3844/ajbbsp.2021.502.508

Zhu Ling-zhi, Guixiang Li, Chunhua Li, Jun Wang

{"title":"Identification of Microbe-Drug Association based on Weighted Profile and Collaborative Matrix Factorization","authors":"Zhu Ling-zhi, Guixiang Li, Chunhua Li, Jun Wang","doi":"10.3844/ajbbsp.2021.502.508","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.502.508","url":null,"abstract":"Corresponding Author: Ying Xiao Hunan Chemical Vocational Technology College, Zhuhou, China Email: lingzhi0825@yeah.net Abstract: Previous studies have shown that diseases are associated with microbe. To explore a more effective treatment for these diseases, unknown microbe-drug associations must be identified. However, existing models to identify microbe-drug association are limited. In our article, a predictive model (WPCMF) is presented for identifying microbe-drug associations based on weighted profile and collaborative matrix factorization. In WPCMF, the Gaussian Interaction Profile (GIP) can be used for computing the similarities of microbe and the drug, respectively. Then we use the Canonical SMILES of drugs to compute the chemical structures similarity of drugs. Two drug similarities are fused into an integrated drug similarity matrix. Weighted profile and collaborative matrix factorization are applied for predicting potential microbe-drug associations. Experimental results show that WPCMF achieves the average Area Under the Curve (AUC) values of 0.9096±0.0028, 0.9195±0.0019 and 0.9236 in 5-fold Cross-Validation (5 CV), 10-fold Cross-Validation (10 CV) and Leave-One-Out-Cross-Validation (LOOCV), respectively, which consistently outperforms other related methods (KATZHMDA, WP, CMF and Kron RLS). We think WPCMF is ideal as a supplement in the field of biomedical research.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84494880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioconversion of Corn Steep Liquor to Biofertilizer by Symbiotic Fermentation 玉米浸泡液共生发酵转化为生物肥料的研究

American Journal of Biochemistry and Biotechnology Pub Date : 2021-04-01 DOI: 10.3844/ajbbsp.2021.478.489

Xiaojie Ren, Zhuangzhuang He, Huihui Peng, Jing Zhang, S. Wang, Yubin Zhao, Xiaoyu Li, Yuanda Song, Xinhe Zhao, Chengye Ma

{"title":"Bioconversion of Corn Steep Liquor to Biofertilizer by Symbiotic Fermentation","authors":"Xiaojie Ren, Zhuangzhuang He, Huihui Peng, Jing Zhang, S. Wang, Yubin Zhao, Xiaoyu Li, Yuanda Song, Xinhe Zhao, Chengye Ma","doi":"10.3844/ajbbsp.2021.478.489","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.478.489","url":null,"abstract":"Chengye Ma School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, China Email: mcycn2002@163.com Abstract: Corn Steep Liquor (CSL) is wastewater from corn starch industry. The huge amount generation made it as a serious environmental problem. But CSL is nutrient-rich with diverse amino acids and other trace elements. In this study, symbiotic biofertilizer was studied using CSL as main substrate. In this study, 9 industrial strains, which were recognized as high performance species in the biofertilizer industry were collected, including four bacillus, four fungus and one yeast species. Three dominant strains were selected finally, depending on the ability to dissolve phosphorus, ability for protein hydrolysis and cell growth. They were Bacillus subtilis 3301, Bacillus licheniformis and Aspergillus Niger. The results expressed that the highest growth cell count of Bacillus subtilis 3301 reached 3.1×10 CFU/mL, the highest protein hydrolysis capacity of Bacillus licheniformis reached 600 mg/L and the highest phosphorus-dissolving capacity of Aspergillus Niger reached 1100 mg/L respectively. Then, symbiotic fermentation of the three strains was carried out both in shake-flask culture and fermentation tank (5 L). Interestingly, series of significant data of symbiotic fermentation were obtained, which were different from individual cultures. Especially, the total growth cell count in the 5 L scale fermenter culture was higher than individual cultures, which proved the mutual promotion effect between different strains. This research would provide a new view to utilize the waste of corn steep liquor as high value-added agricultural products.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72805681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic Analysis of the Erythritol High-Yielding Mutant Strain Yarrowia lipolytica Y44 赤藓糖醇高产解脂耶氏菌Y44的转录组学分析

American Journal of Biochemistry and Biotechnology Pub Date : 2021-04-01 DOI: 10.3844/ajbbsp.2021.433.447

Xiaojie Ren, Heng Ban, Chao Huang, Baoyue Liu, Yuanda Song, Xinhe Zhao, Jiangsen Pei

引用次数: 0

Vitamin D Supplements Tone Down the Progression of Atheromatous Plaque Formation in a Dose-Dependent Manner 维生素D补充剂以剂量依赖的方式降低动脉粥样硬化斑块形成的进展

American Journal of Biochemistry and Biotechnology Pub Date : 2021-03-01 DOI: 10.3844/ajbbsp.2021.362.369

A. Farouk, Khalid M. Mohany, Magdy Algowhary, Lobna A. Abdelzaher, Heba E M El-Deek, Ghada Hosny, T. Saleem

{"title":"Vitamin D Supplements Tone Down the Progression of Atheromatous Plaque Formation in a Dose-Dependent Manner","authors":"A. Farouk, Khalid M. Mohany, Magdy Algowhary, Lobna A. Abdelzaher, Heba E M El-Deek, Ghada Hosny, T. Saleem","doi":"10.3844/ajbbsp.2021.362.369","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.362.369","url":null,"abstract":"Corresponding Author: Tahia Hashim Saleem Department of Biochemistry, Faculty of Medicine, Assiut University, Egypt Email: tahia.h.saleem@aun.edu.eg Abstract: Hypovitaminosis D has a negative impact on the cardiovascular system. We performed the current study to investigate the expression of Vitamin D Receptor (VDR) in atherosclerotic human male aortas and to examine the effect of different doses of Vit. D supplementation on VDR expression in the aortas of male rats fed a High-Fat Diet (HFD). Human participants included 50 atherosclerotic male patients with anterior myocardial infarction and 131 control healthy males. Fifty male Wistar rats were divided into 2 groups: Control (Cont.) group; n = 10 and HFD fed rats; n = 40. The latter was divided equally into 4 subgroups according to the dose of Vit. D supplemented. Vitamin D was supplemented in 3 doses: (0.025, 0.05, 0.075 μg/kg) for a duration of 17 weeks. VDR expression score was immunohistochemically evaluated in aortas of both human and animal study groups. The current study revealed that Vit. D levels were significantly lower in atherosclerotic patients with myocardial infarctions compared to the human control group. Concurrent administration of Vit. D suppresses the progress of atheromatous plaque in rats feed a HFD in a dose-dependent manner. Consequently, it could be concluded that Vit. D supplements are recommended as a prophylaxis in patients at high risk of coronary artery disease.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84970969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Isolation and Screening of Wine Yeasts from Grapes in Yalu River Valley China for Fermentation Performance 鸭绿江流域葡萄产酒酵母的分离筛选及发酵性能研究

American Journal of Biochemistry and Biotechnology Pub Date : 2021-03-01 DOI: 10.3844/ajbbsp.2021.346.353

Xiaochun Yu, Jing Xu, Y. Li, Xian Li

{"title":"Isolation and Screening of Wine Yeasts from Grapes in Yalu River Valley China for Fermentation Performance","authors":"Xiaochun Yu, Jing Xu, Y. Li, Xian Li","doi":"10.3844/ajbbsp.2021.346.353","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.346.353","url":null,"abstract":"Corresponding Author: Jing Xu College of Food Science and Engineering, Tonghua Normal University, Tonghua 134002, China E-mail: 122294352@qq.com Abstract: In order to further explore the diversity of yeast in grape brewing in Yalu River Valley, northern China and to better exploit and utilize the yeast resources in a grape producing area, we investigated soil, grape residue after juice extraction and naturally fermented grape juice samples collected from grape plantations in the Yalu River Valley in this study. Twelve yeast strains were identified using colony and cell morphology and molecular biology. Three Saccharomyces cerevisiae strains from different sources, EN2, BZ1 and EZ2, were selected to determine their acid, Sodium dioxide (SO2), glucose and ethanol tolerance. The results showed that EN2 exhibited significant acid and SO2 tolerance, while BZ1 and EZ2 exhibited significant glucose and ethanol tolerance. These three S. cerevisiae strains showed good growth and fermentation characteristics and could be used as development strains for brewing wine. Results of this study could be useful in breeding, wine yeast-strain research and wine product development.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"162 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91034770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies on Pharmacokinetics and Liver Microsome Metabolism of Nuciferine in Different Species of Animals 荷叶碱在不同动物体内的药代动力学及肝微粒体代谢研究

American Journal of Biochemistry and Biotechnology Pub Date : 2021-03-01 DOI: 10.3844/ajbbsp.2021.328.335

Dong Xiao-chen, Liu Ming-qi, L. Jun, Guan Feng, Xue Ai-chun, Zhao Jin, Ge Jian

{"title":"Studies on Pharmacokinetics and Liver Microsome Metabolism of Nuciferine in Different Species of Animals","authors":"Dong Xiao-chen, Liu Ming-qi, L. Jun, Guan Feng, Xue Ai-chun, Zhao Jin, Ge Jian","doi":"10.3844/ajbbsp.2021.328.335","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.328.335","url":null,"abstract":"Corresponding Author: Ge Jian College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China E-mail: gejian16888@163.com Abstract: This study aimed to compare the characteristics of pharmacokinetics and liver microsome metabolism among different species of animals including rabbit, rat and mouse and healthy New Zealand white rabbits, SPF Sprague Dawley (SD) rats and ICR mice were given intraperitoneal injection of nuciferine at a dose of 50 mg per kg body weight. Blood samples were collected via caudal vein after administration and nuciferine concentrations in different points were determined by the method of HPLC-UV. Then the plasma concentration-time curves were drawn and the pharmacokinetics parameters were calculated by DAS 3.0 software. Besides, liver microsome from rabbit, rats and mice were prepared to study the metabolism of nuciferine in vitro. These results showed that nuciferine fitted two-compartment model in rabbits and rats and one-compartment model in mice. The calculated results of pharmacokinetics parameters showed that the fast absorption of nuciferine exhibited in rabbits, rats and mice, while the elimination was relatively slow. Compared with rabbits and rats, the nuciferine elimination was fastest in mice. The Km value of nuciferine metabolism in vitro was least in mice liver microsome, which suggested that the fastest metabolism of nuciferine existed in mice liver. Therefore, the pharmacokinetics characteristics of nuciferine in different species were significantly different, the elimination of nuciferine is the fastest in mice and the slowest in rabbits, which was in line with the in vitro metabolism trends by liver microsome from different species.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78883745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Inflammatory and Oxidative Stress Biomarkers in Multi Vessels Minimally Invasive Coronary Artery Bypass Graft Surgery Versus Off-Pump Coronary Artery Bypass Graft 多血管微创冠状动脉旁路移植术与非体外循环冠状动脉旁路移植术中炎症和氧化应激生物标志物的研究

American Journal of Biochemistry and Biotechnology Pub Date : 2021-03-01 DOI: 10.3844/ajbbsp.2021.354.361

Mohammed Farouk Abdel-Hafez, A. El-minshawy, A. Taha, A. Mandour, M. G. Mahmoud, Khalid M. Mohany

引用次数: 0