American Journal of Biochemistry and Biotechnology最新文献

{"title":"The Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) Genotypes","authors":"M. Hasan, Fahmid H Bhuiyan, H. Hoque, N. A. Jewel, M. Ashrafuzzaman, S. Prodhan","doi":"10.3844/AJBBSP.2021.148.159","DOIUrl":"https://doi.org/10.3844/AJBBSP.2021.148.159","url":null,"abstract":"Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular and molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization of recalcitrant indica rice genotypes is a critical step for the biotechnological approaches for further development as the optimum concentrations of Plant Growth Regulators (PGRs) for in-vitro regeneration vary from genotype to genotype. Here, 12 important rice genotypes, including local cultivars and varieties, were collected from different regions of Bangladesh and evaluated for their in-vitro regeneration potentiality. Mature dehusked sterilized rice seeds were inoculated on different basal media (MS, N6, B5) supplemented with different concentrations of 2,4-Dichlorophenoxyaceitic acid (2,4-D) for callus induction. Induced calli were transferred to N6 media supplemented with different combinations of 6-Benzylaminopurine (BA) and 1-Napthaleneacetic Acid (NAA) for shoot induction. Regenerated shoots were rooted on half-strength MS media without supplementation of any hormones. Treatments of different concentrations and combinations of plant growth regulators showed varying results. Maximum callus induction frequencies were observed with 2.5-3.0 mg/l supplementation of 2,4-D on N6 media where Saat, Jamaibabu and Nimikhanai showed the highest callus induction frequencies (84.44%; 78.89 and 80% respectively) and maximum shoot initiation was observed at 2.5 mg/l BA +1.0 mg/l NAA and 3.0 mg/l 6-BA +1.5 mg/l NAA supplemented media where rice genotypes BRRI Dhan71, Nayapaijam, Nimikhanai and BRRI Dhan28 showed the highest shoot induction frequencies-86.67, 85.00, 86.67 and 88.33% respectively. Nearly all of the induced shoots showed root formation on half-strength MS media. This study demonstrates variations in regeneration response of the rice genotypes where cultivars Jamaibabu, Nayapaijam and Nimikhanai showed the highest in-vitro regeneration potential.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"42 1","pages":"148-159"},"PeriodicalIF":0.0,"publicationDate":"2021-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81930818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Sorbitol Calcium Chelate on Yield and Calcium Nutrient Absorption of Peanut","authors":"Chang Liu, Yupeng Li, Wen-Can Huo, Tengsheng Li, Qianqian Wei, Mingli Huang, C. Geng, Dongyun Yan","doi":"10.3844/AJBBSP.2021.160.173","DOIUrl":"https://doi.org/10.3844/AJBBSP.2021.160.173","url":null,"abstract":"Sorbitol has the effect of promoting nutrient absorption, so it has the potential to produce excellent fertilizers. In this study, sorbitol calcium chelate was synthesized using sorbitol as the chelating agent. The effects of sorbitol calcium chelate on peanut were investigated, with the goal of promoting peanut yield and improving the utilization of calcium fertilizer. Field experiments were carried out in Jimo, Shandong Province, China. Four sorbitol calcium chelate concentrations (0.4, 0.8, 1.6 and 2.4 g L-1) and two sorbitol non-chelated calcium concentrations (1.6 and 2.4 g L-1) were tested. Compared with the corresponding sorbitol non-chelated calcium fertilizer treatments, the yield of peanuts increased by 10.0 and 1.7% under sorbitol chelated calcium concentrations of 1.6 and 2.4 g L-1, respectively, besides, the calcium and potassium contents of the seed kernel increased by 32.2 and 55.8%, under sorbitol chelated calcium concentrations of 1.60 and 2.40 g L-1, respectively. Sorbitol calcium chelate improved peanut yield and promoted the transformation of sugars to fat in the seed kernel, the 1.6 g L-1 concentration produced the best results. Compared with the blank control, the 1.60 g L-1 sorbitol chelated calcium fertilizer treatment increased peanut yield by 28.6%, fruit number per plant by 46.8%, 100-kernel weight by 20.4%, full fruit number per plant by 55.3%, fat content by 5.0%. In conclusion, sorbitol chelated calcium fertilizer was superior to sorbitol non-chelated calcium fertilizer under the experimental conditions used herein.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"11 1","pages":"160-173"},"PeriodicalIF":0.0,"publicationDate":"2021-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79478967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Bioaccumulation and Detoxification Mechanism of Neodymium on the Shoot of Rice Seedlings","authors":"Kailun Shi, Chengkun Liu, Keliang Lyu, Jie Chen, Xue Wang","doi":"10.3844/AJBBSP.2021.140.147","DOIUrl":"https://doi.org/10.3844/AJBBSP.2021.140.147","url":null,"abstract":"Neodymium (Nd) potentially pollutes environment for its wide usage in agriculture and industry. In this study, the chemical forms of Nd and effect of Nd on rice shoots were studied after rice seedlings were exposed with 0, 1, 10, 100 and1000 μM Nd. The higher concentration of Nd inhibited shoot growth, but the lower concentration of Nd induced shoot growth. Six different chemical forms of Nd were present in shoot and the major forms of Nd were the insoluble phosphate and oxalate Nd. The content of Nd was further detected in the soluble fractions, organelles and cell wallsof rice shoot, respectively. Nd was mainly accumulated in the cell wall of shoot. The higher concentration of Nd decreased the content of Ca, K, Mg, Fe, Mn, Cu and Zn in shoot. Moreover, the activities of antioxidant enzymes and the contents of Reactive Oxygen Species (ROS) were elevated by Nd treatments. Our results showed that the inactive oxalate or phosphate Nd was the major forms of Nd in rice shoot and the efficient sequestration into cell wall was a key detoxification pattern of Nd in the shoot of rice seedlings.With the wide usage of Rare Earth Elements (REEs) in agriculture, the detoxification mechanism of Nd in rice shoot was helpful for assessing the environmental safety of REEs.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"9 1","pages":"140-147"},"PeriodicalIF":0.0,"publicationDate":"2021-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81909157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Soybean Oil on Lipid Metabolism in Mucor circinelloides WJ11 by Metabolomic Analysis","authors":"Caili Sun, A. M. Shah, Junhuan Yang, Chen Wu, Zongmin Wang, Yuanda Song","doi":"10.3844/AJBBSP.2021.130.139","DOIUrl":"https://doi.org/10.3844/AJBBSP.2021.130.139","url":null,"abstract":"Corresponding Author: Yuanda Song Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China Email: ysong@sdut.edu.cn Abstract: Oleaginous fungus Mucor circinelloides utilizes different carbon sources associated with sustainable production of lipids, using soybean oil as carbon source, the fungus accumulated more intracellular lipids. Nonetheless, the metabolic changes in M. circinelloides upon vegetable oil as main carbon source have not been yet reported. Therefore, this study was conducted to investigate the metabolomics of M. circinelloides WJ11 cultivated in mixed glucose and soybean oil as carbon source and to reveal its effects on lipid metabolism. M. circinelloides strain WJ11 was cultured under optimized conditions in a fermenter and the biomass samples were collected after 24 h for lipid metabolite investigation. The frozen biomass samples were subjected to various chromatographic analyses like liquid chromatography mass spectrometry analysis and high resolution-mass spectrometry for metabolite profiling. A total of 438 differential metabolites were identified, of which 48 were up-regulated and 33 were down-regulated. Among them, lysophosphatidic acid and monoglyceride were up-regulated whereas phosphatidylglycerol and lyso-phosphatidylglycerol were down-regulated in the experimental group (soybean oil and glucose as mixed carbon sources) compared with the control group (glucose as single carbon source). Significant changes in metabolite levels correlated to lipid synthesis were identified. This study showed that the addition of soybean oil to the medium favors the triacylglycerol synthesis in M. circinelloides. Our results can also be applied to the investigation of other microorganisms and would contribute to further genetic engineering for higher lipid accumulation in fungi.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"15 1","pages":"130-139"},"PeriodicalIF":0.0,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84584376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Rosemary Collagen Chitosan Blend Membrane Using Response Surface Methodology (RSM)","authors":"Ming He, Yuhan Zhai, Yuqing Zhang, Shuo Xu, Shaoxuan Yu, Haifang Xiao, Yuanda Song","doi":"10.3844/ajbbsp.2021.395.407","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.395.407","url":null,"abstract":"Corresponding Author: Haifang Xiao and Yuanda Song Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China Email: xiaohaifang@sdut.edu.cn ysong@sdut.edu.cn Abstract: The present research provided a formula for the optimization of rosemary/collagen/chitosan blend membrane. Rosemary Extract (RE) was used as additive and membrane was prepared using tensile strength, elongation at break and elongation length as the indexes. The results of single factor experiment showed that the addition ratio of collagen to chitosan, additive content, glycerol content and drying temperature all affected the mechanical properties and thickness of the blend membrane, among which the addition ratio of collagen to chitosan and glycerol content had a great influence. Box-Behnken Design (BBD) of Response Surface Methodology (RSM) was used to optimize the process and the parameters were obtained as follows: The ratio of collagen to chitosan (39:61, v/v), the addition of RE (0.6 mg/mL) and the glycerol content (30.2%). The mechanical properties and thickness of the optimized formula were verified, which proved the feasibility of the scheme and the broad application prospect of rosemary/collagen/chitosan blend membrane in meat packaging industry. The optimal data of tensile strength, elongation at break, elongation length and thickness were 2789.65 g, 49.92%, 117.55 and 0.143 mm, respectively. In the future, the research of blend membrane in food industry will more meet the diversified and high-performance market demand.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85209110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyl Radical-Scavenging Activity-Guided Extraction of Peptides from Oviductus ranae","authors":"Jieyang Shen, Yihui Liu, Jiahao Xu, Shenbo Ye, Xiaoxiao Xu, Yang Liu, Lixue Zheng, Yang Zhang","doi":"10.3844/ajbbsp.2021.361.372","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.361.372","url":null,"abstract":"Yang Zhang School of Biology and Food Engineering/Changshu Institute of Technology, Changshu, China Email: zhangyang@cslg.edu.cn Abstract: Oviductus Ranae (OR) is a traditional Chinese medicinal material that is listed in Chinese Pharmacopeia with proteins the most abundant ingredients. In present work, pepsin/trypsin-based stepwise hydrolysis was applied to extract Antioxidant Peptides from OR (APOR) using hydroxyl radical-scavenging capacity as evaluation index. After single-factor experiment, response surface method coupled with Box-Behnken design were used for optimizing process parameters and achieved the optimal conditions: Pepsin amount of 2.0%, pepsin incubation time of 3.5 h, trypsin amount of 2.0% and trypsin incubation time of 3.0 h, under these conditions, the hydroxyl radical-scavenging rate of obtained APOR was 75.52±1.92%, which was extremely similar to the predicated value with relative deviation of ±1.42%. The half inhibitory concentration of APOR against hydroxyl radical was 3.71±0.71 mg.ml, which was lower than that of proteins from OR (4.85±0.06 mg.mL, disclosed in our previously published paper). The higher antioxidant capacity of APOR could be related to the presence of rich antioxidant amino acids and relatively lower molecular weights, which deserve to be explored in the next future.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"IM-30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84739359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nattokinase Activity Guided Natto Fermentation Process Optimization","authors":"Xiaojie Ren, Chao Huang, Xin Shan, Heng Ban, Baoyue Liu, Yu Du, Yuanda Song, Xinhe Zhao","doi":"10.3844/ajbbsp.2021.423.432","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.423.432","url":null,"abstract":"Corresponding Author: Xinhe Zhao School of Agriculture Engineering and Food Science, Colin Ratledge Center for Microbial Lipids, China E-mail: zhaoxinhe2018@163.com Abstract: The natto kinase in natto has obvious thrombolytic effect, has the advantages of low cost, small side effects, long half-life and can be taken orally, so it has attracted much attention. At present, the research on natto kinase in our country mainly focuses on the selection of natto fermentation strains, the optimization of gene expression conditions and the exploration of thrombolytic mechanism. However, most natto products show that the number of viable bacteria is not high. Natto Kinase activity is low and no breakthrough has been made so far. For this reason, this topic has launched a study on how to improve the activity of natto kinase. This topic optimizes the traditional natto fermentation process and obtains the best fermentation parameters on the basis of enhancing the activity of natto kinase. The experiment first compared and selected two different fermentation strains and determined that Bacillus natto was the dominant fermentation strain. Considering that the water content of soybeans can indirectly affect the activity of natto kinase, the experiment explored the effects of soaking and steaming beans on the water content of soybeans and determined that soaking beans is an influencing factor for the fermentation process. During the fermentation process, soaking time, fermentation temperature, fermentation time and inoculation amount of superior species, were designed to explore the optimal fermentation process that make the highest nano kinase activity. The TAME method is used to accurately detect the activity of natto kinase in the samples after fermentation and the physical properties of the natto before and after fermentation are measured with a physical property test set. The data obtained can quantify the subjective sensory evaluation. Finally, the designexport 11.0 software was used to analyze and optimize the optimal fermentation process parameters with a soaking time of 15 h, a fermentation temperature of 37°C, a fermentation time of 30 h and an inoculum of 6 mL and the natto kinase activity under the combined conditions was measured to be 3 μ·mL.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77344830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction Optimization of Essential Oil from Medicago hispida and Preliminary Inhibitory Mechanism Against Escherichia coli","authors":"Xiulan Chu, Qianfeng Chen, Tong Wang, Xinyu Bai, Chenling Pan, Yang Zhang, Lixue Zheng","doi":"10.3844/ajbbsp.2021.384.394","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.384.394","url":null,"abstract":"Lixue Zheng School of Biology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China Email: zhenglixue@cslg.edu.cn Abstract: In this study, the extraction optimization, characterization, antioxidant evaluation and preliminary inhibitory mechanism against Escherichia coli of the essential oil from dried Medicago hispida (EOMH) were explored to uncover the pending questions that had been raised in our previous work and to enlarge the industrial potential of EOMH production. The optimal parameters for EOMH extraction were: Extraction time of 7 h, particle size of 20 mesh and liquid-tosolid ratio of 30: 1 (mL/g), under which the highest yield of EOMH reached 0.31%. Nineteen constituents were identified in EOMH by gas chromatography mass spectrometry analysis, among which phytol content was up to 53.6%. These structures were further confirmed by infrared spectroscopy analysis. EOMH exerted similar scavenging capacities against Diphenyl Picryl Hydrazinyl (DPPH) and hydroxyl radicals with those of phytol. However, EOMH elicited stronger inhibitory effect on E. coli than that of phytol via shrinking E. coli cells, indicating that other factors, in addition to the antioxidant mechanism would equally affect the inhibitory effect of EOMH against E. coli, which should be deepened in the near further. In summary, present work would provide evidence for the industrial production and application of EOMH.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77376635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Flavor Properties of Sufu by HS-SPME/GC-MS, Electronic Nose and Electronic Tongue Combined with Multivariate Statistical Analysis","authors":"X. Xi, Yanli Ma, Jingxuan Ke, Yin-Zhao Wang, Xiaodong Gu, Yaqiong Liu, Jianfeng Sun, Jianlou Mu","doi":"10.3844/ajbbsp.2021.490.501","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.490.501","url":null,"abstract":"Corresponding Author: Yanli Ma Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology; Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Nanyang Institute of Technology, Nanyang, China Email: xuexi_myl@sina.com Abstract: Flavor is the main quality characteristic of sufu. The flavor of different types of sufu varies greatly, which will affect the preference and selectivity of consumer for sufu. To explore the flavor characteristics and distinguishing methods in different types of sufu, the volatile flavor components and free amino acids of 5 kinds of commercial sufu were determined by HS-SPME/GC-MS and amino acid automatic analyzer, respectively. Moreover, the electronic nose and electronic tongue were used to distinguish the sufu samples. The results showed that a total of 71 volatile aroma compounds were identified, in which esters (28.43-79.17%) were the main flavor substances. The total free amino acid contents in different types of sufu were at a range of 24.8-33.89 mg/g. Bitter amino acids were dominant in all sufu (except GS). PCA of electronic nose and electronic tongue showed that the electronic nose can only distinguish the grey sufu, while the electronic tongue can effectively distinguish among types of sufu. PLS-DA based on aroma and taste substances showed that sufu can be distinguished. According to VIP, fifteen volatile flavor components and five taste amino acids could be used as the main different compounds of sufu. The research results pretended that HS-SPME/GC-MS, electronic tongue technology combined with multivariate statistical analysis method can effectively distinguish sufu. These findings will provide guiding significance to identify the types of sufu and consumers’ purchase products and promote the development of sufu industry to a certain extent.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79453383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium Enriched Peanut Protein Alleviates Alcohol-Induced Liver Damage in Mice by Modulating the Composition of the Gut Microbiota","authors":"Lin Gao, Jia Yuan, Yuhuan Cheng, Mengling Chen, Jihong Wu","doi":"10.3844/ajbbsp.2021.465.477","DOIUrl":"https://doi.org/10.3844/ajbbsp.2021.465.477","url":null,"abstract":"Corresponding Author: Jihong Wu China Agricultural University, No. 17, Qinghua East Road, Haidian District, Beijing 100083, China. E-mail: wjhcau@hotmail.com Abstract: Alcohol Use Disorder (AUD) is one of leading external causes of mortality. Selenium-enriched Peanut Protein (SePP) is a type of Se compound present in Se-enriched plants. Our previous studies showed that SePP possesses potential therapeutic properties and could alleviate alcoholinduced AML-12 cytotoxicity. It spurred us to determine the underlying mechanisms and roles of SePP on gut microbiota. Here, ICR mice were fed either standard diet or ethanol (30%, v/v, 10 mL/kg bw/day) intragastric administration for 8 weeks. The intervention groups were divided into different doses of SePP groups and Se compounds groups, like Selenomethionine (SeMet) group, sodium selenite group. The composition of the gut microbiota was investigated by analyzing 16S rRNA gene sequences. Body weight, lipid metabolism markers, serum insulin and oxidative stress were assessed. Treatment with SePP at a certain dosage (25 μg/kg bw/day in Se) and SeMet reversed diversity loss and community alterations in the gut microbiota of the AUD group, as evidenced by an increased abundance of Firmicutes and decreased abundances of Bacteroidetes and Verrucomicrobia in AUD mice. SePP suppressed the relative abundance of the Rikenellaceae_RC9 gut group and increased those of Lachnospiraceae and Ruminococcaceae, which are associated with lipid metabolism and Short-Chain Fatty Acids (SCFAs) production. The study suggested that SePP has the potential to be used as a supplement for alleviating alcohol-induced liver damage.","PeriodicalId":7412,"journal":{"name":"American Journal of Biochemistry and Biotechnology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78990695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}