Life medicine最新文献_第8页

Metabolic orchestration of drug-tolerant persister cells in cancer. 癌症中耐药持久细胞的代谢协调。

Life medicine Pub Date : 2024-12-07 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae040

Meng Nie, Zeping Hu

引用次数: 0

Targeted delivery of a cationic dendrimer with a plaque-homing peptide for the treatment of atherosclerosis. 带斑块归巢肽的阳离子树状大分子靶向递送治疗动脉粥样硬化。

Life medicine Pub Date : 2024-11-25 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae039

Tarik Zahr, Tianyu Li, Divya Bhansali, Qianfen Wan, Kam W Leong, Li Qiang

引用次数: 0

4D live tracing reveals distinct movement trajectories of meiotic chromosomes. 4D实时追踪显示了减数分裂染色体明显的运动轨迹。

Life medicine Pub Date : 2024-11-13 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae038

Peng Xie, Shiqi Zhu, Jin Zhang, Xinrui Wang, Xu Jiang, Feng Xiong, Linjin Chen, Ke Fang, Yuanhui Ji, Beihong Zheng, Lincui Da, Hua Cao, Yan Sun, Zhuojuan Luo, Chengqi Lin

引用次数: 0

Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients. 综合转录组学分析揭示了复发性着床失败患者分泌性子宫内膜中免疫浸润和促炎细胞因子的失调。

Life medicine Pub Date : 2024-10-21 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae036

Ping Zhou, Dan Mo, Hanji Huang, Jiaqi Xu, Baoying Liao, Yinxue Wang, Di Mao, Zhonghong Zeng, Ziying Huang, Chao Zhang, Yihua Yang, Yang Yu, Heng Pan, Rong Li

{"title":"Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients.","authors":"Ping Zhou, Dan Mo, Hanji Huang, Jiaqi Xu, Baoying Liao, Yinxue Wang, Di Mao, Zhonghong Zeng, Ziying Huang, Chao Zhang, Yihua Yang, Yang Yu, Heng Pan, Rong Li","doi":"10.1093/lifemedi/lnae036","DOIUrl":"10.1093/lifemedi/lnae036","url":null,"abstract":"Recurrent implantation failure (RIF) is a leading impediment to assisted reproductive technology, yet the underlying pathogenesis of RIF remains elusive. Recent studies have sought to uncover novel biomarkers and etiological factors of RIF by profiling transcriptomes of endometrial samples. Nonetheless, the inherent heterogeneity among published studies and a scarcity of experimental validations hinder the identification of robust markers of RIF. Hence, we integrated six publicly accessible datasets with 209 samples, including microarray profiles of endometrial samples in the secretory phase. After removing batch effects, we identified 175 differentially expressed genes. Gene set enrichment analysis identified dysregulation of immunological pathways in RIF. We also observed altered immune infiltration and pro-inflammatory cytokines in RIF. Protein-protein interaction network analysis identified ten hub genes, representing two co-expression modules significantly related to RIF. Knockdown of ENTPD3, one of the hub genes, promoted the epithelial-mesenchymal transition process and resulted in elevated levels of pro-inflammatory cytokines. Collectively, our study reveals abnormal gene expressions involving the regulation of epithelial-mesenchymal transition and immune status in RIF, providing valuable insights into its pathogenesis.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 5","pages":"lnae036"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenotransplantation: How close are we to clinical applications? 异种器官移植：我们离临床应用还有多远？

Life medicine Pub Date : 2024-10-03 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae037

Hang Zhang, Yuan Chang, Jiangping Song

引用次数: 0

mRNA therapy: a precise and efficient approach for disease treatment. mRNA疗法：一种精确有效的疾病治疗方法。

Life medicine Pub Date : 2024-09-14 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae035

Jia-Yi Zhou, Ying Yang

引用次数: 0

The complex landscape of immune dysregulation in multisystem inflammatory syndrome in children with COVID-19. COVID-19儿童多系统炎症综合征免疫失调的复杂格局

Life medicine Pub Date : 2024-09-13 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae034

Jing Guo, Lie Wang

引用次数: 0

Comparative dissection of transcriptional landscapes of human iPSC-NK differentiation and NK cell development. 人iPSC-NK分化与NK细胞发育转录图谱的比较分析。

Life medicine Pub Date : 2024-09-06 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae032

Li Zhang, Taylor M Weiskittel, Yuqing Zhu, Dixuan Xue, Hailing Zhang, Yuxuan Shen, Hua Yu, Jingyu Li, Linxiao Hou, Hongshan Guo, Zhijun Dai, Hu Li, Jin Zhang

{"title":"Comparative dissection of transcriptional landscapes of human iPSC-NK differentiation and NK cell development.","authors":"Li Zhang, Taylor M Weiskittel, Yuqing Zhu, Dixuan Xue, Hailing Zhang, Yuxuan Shen, Hua Yu, Jingyu Li, Linxiao Hou, Hongshan Guo, Zhijun Dai, Hu Li, Jin Zhang","doi":"10.1093/lifemedi/lnae032","DOIUrl":"10.1093/lifemedi/lnae032","url":null,"abstract":"Clinical and preclinical research has demonstrated that iPSC-derived NK (iNK) cells have a high therapeutic potential, yet poor understanding of the detailed process of their differentiation in vitro and their counterpart cell development in vivo has hindered therapeutic iNK cell production and engineering. Here we dissect the crucial differentiation of both fetal liver NK cells and iNK cells to enable the rational design of advanced iNK production protocols. We use a comparative analysis of single-cell RNA-seq (scRNA-seq) to pinpoint key factors lacking in the induced setting which we hypothesized would hinder iNK differentiation and/ or functionality. By analyzing key transcription factor regulatory networks, we discovered the importance of TBX21, EOMES, and STAT5A in the differentiation timeline. This analysis provides a blueprint for further engineering new iPSC lines to obtain iNK cells with enhanced functions. We validated this approach by creating a new line of STAT5A-iPSCs which can be differentiated to STAT5A-expressing macrophages with both NK cell and macrophage features such as perforin production, phagocytosis, and anti-tumor functions.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 4","pages":"lnae032"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system. 解读眼表外胚层分化系统中动态单细胞转录景观。

Life medicine Pub Date : 2024-09-05 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae033

Canwei Zhang, Zesong Lin, Yankun Yu, Siqi Wu, Huaxing Huang, Ying Huang, Jiafeng Liu, Kunlun Mo, Jieying Tan, Zhuo Han, Mingsen Li, Wei Zhao, Hong Ouyang, Xiangjun Chen, Li Wang

{"title":"Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system.","authors":"Canwei Zhang, Zesong Lin, Yankun Yu, Siqi Wu, Huaxing Huang, Ying Huang, Jiafeng Liu, Kunlun Mo, Jieying Tan, Zhuo Han, Mingsen Li, Wei Zhao, Hong Ouyang, Xiangjun Chen, Li Wang","doi":"10.1093/lifemedi/lnae033","DOIUrl":"10.1093/lifemedi/lnae033","url":null,"abstract":"The ocular surface ectoderm (OSE) is essential for the development of the ocular surface, yet the molecular mechanisms driving its differentiation are not fully understood. In this study, we used single-cell transcriptomic analysis to explore the dynamic cellular trajectories and regulatory networks during the in vitro differentiation of embryonic stem cells (ESCs) into the OSE lineage. We identified nine distinct cell subpopulations undergoing differentiation along three main developmental branches: neural crest, neuroectodermal, and surface ectodermal lineages. Key marker gene expression, transcription factor activity, and signaling pathway insights revealed stepwise transitions from undifferentiated ESCs to fate-specified cell types, including a PAX6 + TP63 + population indicative of OSE precursors. Comparative analysis with mouse embryonic development confirmed the model's accuracy in mimicking in vivo epiblast-to-surface ectoderm dynamics. By integrating temporal dynamics of transcription factor activation and cell-cell communication, we constructed a comprehensive molecular atlas of the differentiation pathway from ESCs to distinct ectodermal lineages. This study provides new insights into the cellular heterogeneity and regulatory mechanisms of OSE development, aiding the understanding of ocular surface biology and the design of cell-based therapies for ocular surface disorders.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 5","pages":"lnae033"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the function of epigenetic regulation in the inflammation of non-alcoholic fatty liver disease. 表观遗传调控在非酒精性脂肪肝炎症中的作用研究。

Life medicine Pub Date : 2024-08-31 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae030

Lin Sun, Zhensheng Yue, Lin Wang

{"title":"Research on the function of epigenetic regulation in the inflammation of non-alcoholic fatty liver disease.","authors":"Lin Sun, Zhensheng Yue, Lin Wang","doi":"10.1093/lifemedi/lnae030","DOIUrl":"10.1093/lifemedi/lnae030","url":null,"abstract":"Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver condition, characterized by a spectrum that progresses from simple hepatic steatosis to nonalcoholic steatohepatitis, which may eventually lead to cirrhosis and hepatocellular carcinoma. The precise pathogenic mechanisms underlying NAFLD and its related metabolic disturbances remain elusive. Epigenetic modifications, which entail stable transcriptional changes without altering the DNA sequence, are increasingly recognized as pivotal. The principal forms of epigenetic modifications include DNA methylation, histone modifications, chromatin remodeling, and noncoding RNAs. These alterations participate in the regulation of hepatic lipid metabolism, insulin resistance, mitochondrial injury, oxidative stress response, and release of inflammatory cytokines, all of which are associated with the onset and progression of NAFLD. This review discussed recent advances in understanding the potential epigenetic regulation of inflammation in NAFLD. Unraveling these epigenetic mechanisms may facilitate the identification of early diagnostic biomarkers and the development of targeted therapeutic strategies for NAFLD.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 4","pages":"lnae030"},"PeriodicalIF":0.0,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0