Life medicine最新文献_第2页

4D live tracing reveals distinct movement trajectories of meiotic chromosomes.

Life medicine Pub Date : 2024-11-13 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae038

Peng Xie, Shiqi Zhu, Jin Zhang, Xinrui Wang, Xu Jiang, Feng Xiong, Linjin Chen, Ke Fang, Yuanhui Ji, Beihong Zheng, Lincui Da, Hua Cao, Yan Sun, Zhuojuan Luo, Chengqi Lin

引用次数: 0

Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients.

Life medicine Pub Date : 2024-10-21 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae036

Ping Zhou, Dan Mo, Hanji Huang, Jiaqi Xu, Baoying Liao, Yinxue Wang, Di Mao, Zhonghong Zeng, Ziying Huang, Chao Zhang, Yihua Yang, Yang Yu, Heng Pan, Rong Li

{"title":"Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients.","authors":"Ping Zhou, Dan Mo, Hanji Huang, Jiaqi Xu, Baoying Liao, Yinxue Wang, Di Mao, Zhonghong Zeng, Ziying Huang, Chao Zhang, Yihua Yang, Yang Yu, Heng Pan, Rong Li","doi":"10.1093/lifemedi/lnae036","DOIUrl":"10.1093/lifemedi/lnae036","url":null,"abstract":"Recurrent implantation failure (RIF) is a leading impediment to assisted reproductive technology, yet the underlying pathogenesis of RIF remains elusive. Recent studies have sought to uncover novel biomarkers and etiological factors of RIF by profiling transcriptomes of endometrial samples. Nonetheless, the inherent heterogeneity among published studies and a scarcity of experimental validations hinder the identification of robust markers of RIF. Hence, we integrated six publicly accessible datasets with 209 samples, including microarray profiles of endometrial samples in the secretory phase. After removing batch effects, we identified 175 differentially expressed genes. Gene set enrichment analysis identified dysregulation of immunological pathways in RIF. We also observed altered immune infiltration and pro-inflammatory cytokines in RIF. Protein-protein interaction network analysis identified ten hub genes, representing two co-expression modules significantly related to RIF. Knockdown of ENTPD3, one of the hub genes, promoted the epithelial-mesenchymal transition process and resulted in elevated levels of pro-inflammatory cytokines. Collectively, our study reveals abnormal gene expressions involving the regulation of epithelial-mesenchymal transition and immune status in RIF, providing valuable insights into its pathogenesis.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 5","pages":"lnae036"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenotransplantation: How close are we to clinical applications?

Life medicine Pub Date : 2024-10-03 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae037

Hang Zhang, Yuan Chang, Jiangping Song

引用次数: 0

mRNA therapy: a precise and efficient approach for disease treatment.

Life medicine Pub Date : 2024-09-14 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae035

Jia-Yi Zhou, Ying Yang

引用次数: 0

The complex landscape of immune dysregulation in multisystem inflammatory syndrome in children with COVID-19.

Life medicine Pub Date : 2024-09-13 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae034

Jing Guo, Lie Wang

引用次数: 0

Comparative dissection of transcriptional landscapes of human iPSC-NK differentiation and NK cell development.

Life medicine Pub Date : 2024-09-06 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae032

Li Zhang, Taylor M Weiskittel, Yuqing Zhu, Dixuan Xue, Hailing Zhang, Yuxuan Shen, Hua Yu, Jingyu Li, Linxiao Hou, Hongshan Guo, Zhijun Dai, Hu Li, Jin Zhang

{"title":"Comparative dissection of transcriptional landscapes of human iPSC-NK differentiation and NK cell development.","authors":"Li Zhang, Taylor M Weiskittel, Yuqing Zhu, Dixuan Xue, Hailing Zhang, Yuxuan Shen, Hua Yu, Jingyu Li, Linxiao Hou, Hongshan Guo, Zhijun Dai, Hu Li, Jin Zhang","doi":"10.1093/lifemedi/lnae032","DOIUrl":"10.1093/lifemedi/lnae032","url":null,"abstract":"Clinical and preclinical research has demonstrated that iPSC-derived NK (iNK) cells have a high therapeutic potential, yet poor understanding of the detailed process of their differentiation in vitro and their counterpart cell development in vivo has hindered therapeutic iNK cell production and engineering. Here we dissect the crucial differentiation of both fetal liver NK cells and iNK cells to enable the rational design of advanced iNK production protocols. We use a comparative analysis of single-cell RNA-seq (scRNA-seq) to pinpoint key factors lacking in the induced setting which we hypothesized would hinder iNK differentiation and/ or functionality. By analyzing key transcription factor regulatory networks, we discovered the importance of TBX21, EOMES, and STAT5A in the differentiation timeline. This analysis provides a blueprint for further engineering new iPSC lines to obtain iNK cells with enhanced functions. We validated this approach by creating a new line of STAT5A-iPSCs which can be differentiated to STAT5A-expressing macrophages with both NK cell and macrophage features such as perforin production, phagocytosis, and anti-tumor functions.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 4","pages":"lnae032"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system.

Life medicine Pub Date : 2024-09-05 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae033

Canwei Zhang, Zesong Lin, Yankun Yu, Siqi Wu, Huaxing Huang, Ying Huang, Jiafeng Liu, Kunlun Mo, Jieying Tan, Zhuo Han, Mingsen Li, Wei Zhao, Hong Ouyang, Xiangjun Chen, Li Wang

{"title":"Deciphering the dynamic single-cell transcriptional landscape in the ocular surface ectoderm differentiation system.","authors":"Canwei Zhang, Zesong Lin, Yankun Yu, Siqi Wu, Huaxing Huang, Ying Huang, Jiafeng Liu, Kunlun Mo, Jieying Tan, Zhuo Han, Mingsen Li, Wei Zhao, Hong Ouyang, Xiangjun Chen, Li Wang","doi":"10.1093/lifemedi/lnae033","DOIUrl":"10.1093/lifemedi/lnae033","url":null,"abstract":"The ocular surface ectoderm (OSE) is essential for the development of the ocular surface, yet the molecular mechanisms driving its differentiation are not fully understood. In this study, we used single-cell transcriptomic analysis to explore the dynamic cellular trajectories and regulatory networks during the in vitro differentiation of embryonic stem cells (ESCs) into the OSE lineage. We identified nine distinct cell subpopulations undergoing differentiation along three main developmental branches: neural crest, neuroectodermal, and surface ectodermal lineages. Key marker gene expression, transcription factor activity, and signaling pathway insights revealed stepwise transitions from undifferentiated ESCs to fate-specified cell types, including a PAX6 + TP63 + population indicative of OSE precursors. Comparative analysis with mouse embryonic development confirmed the model's accuracy in mimicking in vivo epiblast-to-surface ectoderm dynamics. By integrating temporal dynamics of transcription factor activation and cell-cell communication, we constructed a comprehensive molecular atlas of the differentiation pathway from ESCs to distinct ectodermal lineages. This study provides new insights into the cellular heterogeneity and regulatory mechanisms of OSE development, aiding the understanding of ocular surface biology and the design of cell-based therapies for ocular surface disorders.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 5","pages":"lnae033"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research on the function of epigenetic regulation in the inflammation of non-alcoholic fatty liver disease.

Life medicine Pub Date : 2024-08-31 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae030

Lin Sun, Zhensheng Yue, Lin Wang

{"title":"Research on the function of epigenetic regulation in the inflammation of non-alcoholic fatty liver disease.","authors":"Lin Sun, Zhensheng Yue, Lin Wang","doi":"10.1093/lifemedi/lnae030","DOIUrl":"10.1093/lifemedi/lnae030","url":null,"abstract":"Nonalcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver condition, characterized by a spectrum that progresses from simple hepatic steatosis to nonalcoholic steatohepatitis, which may eventually lead to cirrhosis and hepatocellular carcinoma. The precise pathogenic mechanisms underlying NAFLD and its related metabolic disturbances remain elusive. Epigenetic modifications, which entail stable transcriptional changes without altering the DNA sequence, are increasingly recognized as pivotal. The principal forms of epigenetic modifications include DNA methylation, histone modifications, chromatin remodeling, and noncoding RNAs. These alterations participate in the regulation of hepatic lipid metabolism, insulin resistance, mitochondrial injury, oxidative stress response, and release of inflammatory cytokines, all of which are associated with the onset and progression of NAFLD. This review discussed recent advances in understanding the potential epigenetic regulation of inflammation in NAFLD. Unraveling these epigenetic mechanisms may facilitate the identification of early diagnostic biomarkers and the development of targeted therapeutic strategies for NAFLD.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 4","pages":"lnae030"},"PeriodicalIF":0.0,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell surface protein-protein interaction profiling for biological network analysis and novel target discovery.

Life medicine Pub Date : 2024-08-29 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae031

Jiaojiao Chen, Maoxin Fang, Yuwei Li, Haodong Ding, Xinyu Zhang, Xiaoyi Jiang, Jinlan Zhang, Chengcheng Zhang, Zhigang Lu, Min Luo

{"title":"Cell surface protein-protein interaction profiling for biological network analysis and novel target discovery.","authors":"Jiaojiao Chen, Maoxin Fang, Yuwei Li, Haodong Ding, Xinyu Zhang, Xiaoyi Jiang, Jinlan Zhang, Chengcheng Zhang, Zhigang Lu, Min Luo","doi":"10.1093/lifemedi/lnae031","DOIUrl":"10.1093/lifemedi/lnae031","url":null,"abstract":"The secretome is composed of cell surface membrane proteins and extracellular secreted proteins that are synthesized via secretory machinery, accounting for approximately one-third of human protein-encoding genes and playing central roles in cellular communication with the external environment. Secretome protein-protein interactions (SPPIs) mediate cell proliferation, apoptosis, and differentiation, as well as stimulus- or cell-specific responses that regulate a diverse range of biological processes. Aberrant SPPIs are associated with diseases including cancer, immune disorders, and illness caused by infectious pathogens. Identifying the receptor/ligand for a secretome protein or pathogen can be a challenging task, and many SPPIs remain obscure, with a large number of orphan receptors and ligands, as well as viruses with unknown host receptors, populating the SPPI network. In addition, proteins with known receptors/ligands may also interact with alternative uncharacterized partners and exert context-dependent effects. In the past few decades, multiple varied approaches have been developed to identify SPPIs, and these methods have broad applications in both basic and translational research. Here, we review and discuss the technologies for SPPI profiling and the application of these technologies in identifying novel targets for immunotherapy and anti-infectious agents.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 4","pages":"lnae031"},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global insights into aging: a multidisciplinary approach to understanding and addressing age-related challenges.

Life medicine Pub Date : 2024-08-20 eCollection Date: 2024-06-01 DOI: 10.1093/lifemedi/lnae029

Si-Yi Su, Chuting He, Jie Ren, Moshi Song

引用次数: 0