Life medicine最新文献_第2页

Single-cell RNA sequencing reveals the intercellular crosstalk and the regulatory landscape of stromal cells during the whole life of the mouse ovary. 单细胞RNA测序揭示了小鼠卵巢整个生命周期间质细胞的细胞间串扰和调控景观。

Life medicine Pub Date : 2024-12-28 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae041

Wan Jiang, Wenya Sun, Yue Peng, Hao Xu, Haonan Fan, Xin Jin, Yue Xiao, Yuxiang Wang, Pin Yang, Wenjie Shu, Jing Li

{"title":"Single-cell RNA sequencing reveals the intercellular crosstalk and the regulatory landscape of stromal cells during the whole life of the mouse ovary.","authors":"Wan Jiang, Wenya Sun, Yue Peng, Hao Xu, Haonan Fan, Xin Jin, Yue Xiao, Yuxiang Wang, Pin Yang, Wenjie Shu, Jing Li","doi":"10.1093/lifemedi/lnae041","DOIUrl":"10.1093/lifemedi/lnae041","url":null,"abstract":"The heterogeneity of ovarian mesenchymal/stromal cells has just been revealed in both mice and humans. However, it remains unclear about the cellular development trace and the intercellular communication network in the whole life of the ovary. In the study, we integrated ours and published single-cell RNA sequencing data from E11.5 (embryonic day 11.5) until M12 (12-month-old) ovaries to show the dynamics of somatic cells along the developmental timeline. The intercellular crosstalk among somatic cell types was depicted with collagen signaling pathway as the most outgoing signals from stromal cells. We identified mesenchymal progenitor cells (CD24+) as the origin of stromal cells. Although their numbers decreased significantly in adults, the cells served as the major signal sender until ovarian senescence. Moreover, the ovarian injury could activate these stem cells and induce stroma remodeling in the aged ovary. Thus, mesenchymal progenitor cells may represent a new strategy to delay ovarian aging in the future.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 6","pages":"lnae041"},"PeriodicalIF":0.0,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intestinal stem cells in intestinal homeostasis and colorectal tumorigenesis. 肠道干细胞在肠道稳态和结直肠肿瘤发生中的作用。

Life medicine Pub Date : 2024-12-25 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae042

Gaoli Shi, Yang Li, Haihong Shen, Qiankun He, Pingping Zhu

引用次数: 0

GlycoRNAs: more than the intersection of glycobiology and RNA biology. GlycoRNAs：不仅仅是糖生物学和RNA生物学的交叉。

Life medicine Pub Date : 2024-12-12 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae044

Haidong Li, Ruijin Zhang, Runsheng Chen, Jianjun Luo

引用次数: 0

DDO1002, an NRF2-KEAP1 inhibitor, improves hematopoietic stem cell aging and stress response. NRF2-KEAP1抑制剂DDO1002可改善造血干细胞衰老和应激反应。

Life medicine Pub Date : 2024-12-12 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae043

Yuwen Li, Aiwei Wu, Xinrong Jin, Haiping Shen, Chenyan Zhao, Xiao Yi, Hui Nie, Mingwei Wang, Shouchun Yin, Hongna Zuo, Zhenyu Ju, Zhenyu Jiang, Hu Wang

{"title":"DDO1002, an NRF2-KEAP1 inhibitor, improves hematopoietic stem cell aging and stress response.","authors":"Yuwen Li, Aiwei Wu, Xinrong Jin, Haiping Shen, Chenyan Zhao, Xiao Yi, Hui Nie, Mingwei Wang, Shouchun Yin, Hongna Zuo, Zhenyu Ju, Zhenyu Jiang, Hu Wang","doi":"10.1093/lifemedi/lnae043","DOIUrl":"10.1093/lifemedi/lnae043","url":null,"abstract":"Oxidative stress diminishes the functionality of hematopoietic stem cells (HSCs) as age advances, with heightened reactive oxygen species (ROS) levels exacerbating DNA damage, cellular senescence, and hematopoietic impairment. DDO1002, a potent inhibitor of the NRF2-KEAP1 pathway, modulates the expression of antioxidant genes. Yet, the extent to which it mitigates hematopoietic decline post-total body irradiation (TBI) or in the context of aging remains to be elucidated. Our study has elucidated the role of DDO1002 in modulating NRF2 activity, which, in turn, activates the NRF2-driven antioxidant response element (ARE) signaling cascade. This activation can diminish intracellular levels of ROS, thereby attenuating cellular senescence. In addition, DDO1002 has been demonstrated to ameliorate DNA damage and avert HSC apoptosis, underscoring its potential to mitigate hematopoietic injury precipitated by TBI. Competitive transplantation assay revealed that the administration of DDO1002 can improve the reconstitution and self-renewal capacity of HSCs in aged mice. Single-cell sequencing analysis elucidated that DDO1002 treatment attenuated intracellular inflammatory signaling pathways and mitigated ROS pathway in aged HSCs, suggesting its potential to restore the viability of these cells. Consequently, DDO1002 effectively activated the NRF2-ARE pathway, delaying cellular senescence and ameliorating impaired hematopoiesis, thereby demonstrating its potential as a therapeutic agent for age-related hematopoietic disorders.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 6","pages":"lnae043"},"PeriodicalIF":0.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic orchestration of drug-tolerant persister cells in cancer. 癌症中耐药持久细胞的代谢协调。

Life medicine Pub Date : 2024-12-07 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae040

Meng Nie, Zeping Hu

引用次数: 0

Targeted delivery of a cationic dendrimer with a plaque-homing peptide for the treatment of atherosclerosis. 带斑块归巢肽的阳离子树状大分子靶向递送治疗动脉粥样硬化。

Life medicine Pub Date : 2024-11-25 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae039

Tarik Zahr, Tianyu Li, Divya Bhansali, Qianfen Wan, Kam W Leong, Li Qiang

引用次数: 0

4D live tracing reveals distinct movement trajectories of meiotic chromosomes. 4D实时追踪显示了减数分裂染色体明显的运动轨迹。

Life medicine Pub Date : 2024-11-13 eCollection Date: 2024-12-01 DOI: 10.1093/lifemedi/lnae038

Peng Xie, Shiqi Zhu, Jin Zhang, Xinrui Wang, Xu Jiang, Feng Xiong, Linjin Chen, Ke Fang, Yuanhui Ji, Beihong Zheng, Lincui Da, Hua Cao, Yan Sun, Zhuojuan Luo, Chengqi Lin

引用次数: 0

Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients. 综合转录组学分析揭示了复发性着床失败患者分泌性子宫内膜中免疫浸润和促炎细胞因子的失调。

Life medicine Pub Date : 2024-10-21 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae036

Ping Zhou, Dan Mo, Hanji Huang, Jiaqi Xu, Baoying Liao, Yinxue Wang, Di Mao, Zhonghong Zeng, Ziying Huang, Chao Zhang, Yihua Yang, Yang Yu, Heng Pan, Rong Li

{"title":"Integrated transcriptomic analysis reveals dysregulated immune infiltration and pro-inflammatory cytokines in the secretory endometrium of recurrent implantation failure patients.","authors":"Ping Zhou, Dan Mo, Hanji Huang, Jiaqi Xu, Baoying Liao, Yinxue Wang, Di Mao, Zhonghong Zeng, Ziying Huang, Chao Zhang, Yihua Yang, Yang Yu, Heng Pan, Rong Li","doi":"10.1093/lifemedi/lnae036","DOIUrl":"10.1093/lifemedi/lnae036","url":null,"abstract":"Recurrent implantation failure (RIF) is a leading impediment to assisted reproductive technology, yet the underlying pathogenesis of RIF remains elusive. Recent studies have sought to uncover novel biomarkers and etiological factors of RIF by profiling transcriptomes of endometrial samples. Nonetheless, the inherent heterogeneity among published studies and a scarcity of experimental validations hinder the identification of robust markers of RIF. Hence, we integrated six publicly accessible datasets with 209 samples, including microarray profiles of endometrial samples in the secretory phase. After removing batch effects, we identified 175 differentially expressed genes. Gene set enrichment analysis identified dysregulation of immunological pathways in RIF. We also observed altered immune infiltration and pro-inflammatory cytokines in RIF. Protein-protein interaction network analysis identified ten hub genes, representing two co-expression modules significantly related to RIF. Knockdown of ENTPD3, one of the hub genes, promoted the epithelial-mesenchymal transition process and resulted in elevated levels of pro-inflammatory cytokines. Collectively, our study reveals abnormal gene expressions involving the regulation of epithelial-mesenchymal transition and immune status in RIF, providing valuable insights into its pathogenesis.","PeriodicalId":74073,"journal":{"name":"Life medicine","volume":"3 5","pages":"lnae036"},"PeriodicalIF":0.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143054565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenotransplantation: How close are we to clinical applications? 异种器官移植：我们离临床应用还有多远？

Life medicine Pub Date : 2024-10-03 eCollection Date: 2024-10-01 DOI: 10.1093/lifemedi/lnae037

Hang Zhang, Yuan Chang, Jiangping Song

引用次数: 0

mRNA therapy: a precise and efficient approach for disease treatment. mRNA疗法：一种精确有效的疾病治疗方法。

Life medicine Pub Date : 2024-09-14 eCollection Date: 2024-08-01 DOI: 10.1093/lifemedi/lnae035

Jia-Yi Zhou, Ying Yang

引用次数: 0