Journal of nanotechnology in engineering and medicine最新文献

{"title":"Single-Crystalline, Nanoporous Gallium Nitride Films With Fine Tuning of Pore Size for Stem Cell Engineering.","authors":"Lin Han,&nbsp;Jing Zhou,&nbsp;Yubing Sun,&nbsp;Yu Zhang,&nbsp;Jung Han,&nbsp;Jianping Fu,&nbsp;Rong Fan","doi":"10.1115/1.4030615","DOIUrl":"https://doi.org/10.1115/1.4030615","url":null,"abstract":"<p><p>Single-crystalline nanoporous gallium nitride (GaN) thin films were fabricated with the pore size readily tunable in 20-100 nm. Uniform adhesion and spreading of human mesenchymal stem cells (hMSCs) seeded on these thin films peak on the surface with pore size of 30 nm. Substantial cell elongation emerges as pore size increases to ∼80 nm. The osteogenic differentiation of hMSCs occurs preferentially on the films with 30 nm sized nanopores, which is correlated with the optimum condition for cell spreading, which suggests that adhesion, spreading, and stem cell differentiation are interlinked and might be coregulated by nanotopography.</p>","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 4","pages":"0410041-410049"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4030615","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33926044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Nanoparticle-Embedded Chitosan Sponge for Topical and Local Administration of Chemotherapeutic Agents.","authors":"Manijeh Goldberg, Aaron Manzi, Erkin Aydin, Gurtej Singh, Payam Khoshkenar, Amritpreet Birdi, Brandon LaPorte, Alejandro Krauskopf, Geralle Powell, Julie Chen, Robert Langer","doi":"10.1115/1.4030899","DOIUrl":"10.1115/1.4030899","url":null,"abstract":"<p><p>The following work describes the development of a novel noninvasive transmucosal drug delivery system, the chitosan sponge matrix (CSM). It is composed of cationic chitosan (CS) nanoparticles (NPs) that encapsulate cisplatin (CDDP) embedded within a polymeric mucoadhesive CS matrix. CSM is designed to swell up when exposed to moisture, facilitating release of the NPs via diffusion across the matrix. CSM is intended to be administered topically and locally to mucosal tissues, with its initial indication being oral cancer (OC). Currently, intravenous (IV) administered CDDP is the gold standard chemotherapeutic agent used in the treatment of OC. However, its clinical use has been limited by its renal and hemotoxicity profile. We aim to locally administer CDDP via encapsulation in CS NPs and deliver them directly to the oral cavity with CSM. It is hypothesized that such a delivery device will greatly reduce any systemic toxicity and increase antitumor efficacy. This paper describes the methods for developing CSM and maintaining the integrity of CDDP NPs embedded in the CSM.</p>","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 4","pages":"0409051-4090511"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4547506/pdf/nano-15-1033_040905.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33975137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotopographic Biomaterials for Isolation of Circulating Tumor Cells","authors":"Weiyi Qian, Yan Zhang, A. Gordon, Weiqiang Chen","doi":"10.1115/1.4030420","DOIUrl":"https://doi.org/10.1115/1.4030420","url":null,"abstract":"Circulating tumor cells (CTCs) shed from the primary tumor mass and circulating in the bloodstream of patients are believed to be vital to understand of cancer metastasis and progression. Capture and release of CTCs for further enumeration and molecular characterization holds the key for early cancer diagnosis, prognosis and therapy evaluation. However, detection of CTCs is challenging due to their rarity, heterogeneity and the increasing demand of viable CTCs for downstream biological analysis. Nanotopographic biomaterial-based microfluidic systems are emerging as promising tools for CTC capture with improved capture efficiency, purity, throughput and retrieval of viable CTCs. This review offers a brief overview of the recent advances in this field, including CTC detection technologies based on nanotopographic biomaterials and relevant nanofabrication methods. Additionally, the possible intracellular mechanisms of the intrinsic nanotopography sensitive responses that lead to the enhanced CTC capture are explored.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"040901"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4030420","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63489751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Zn-Doped Manganese Ferrite Nanoparticles Via Coprecipitation Method for Magnetic Resonance Imaging Contrast Agent","authors":"F. Salehpour, A. Khorramdin, H. Shokrollahi, A. Pezeshki, F. Mirzaei, N. Nader","doi":"10.1115/1.4029855","DOIUrl":"https://doi.org/10.1115/1.4029855","url":null,"abstract":"Two different preparations of biocompatible magnetic nanoparticles (MNPs), both (MnFe2O4 and Mn0.91Zn0.09Fe2O4) coated with methoxy polyethylene glycol aldehyde (m-PEG-CHO) were prepared through coprecipitation method. The prepared powder was reanalyzed for material structure with an X-ray diffractometer (XRD) and for particle size using a transition electron microscope (TEM). Magnetic saturation (MS) and coercivity (HC) of the formed particles were examined by a vibrating sample magnetometer (VSM). Surface structure of the samples was characterized by Fourier transform infrared spectroscopy (FTIR). Biocompatible ferrofluids were intravenously injected into four rabbits. Then the magnetic resonance (MR) images of brain were obtained by magnetic resonance imaging (MRI) experiments before and after intravenous injection of ferrofluids. The MNPs demonstrate super paramagnetic behavior with a spinel structure measuring 30–40 nm in size. Doping of these magnetite nanoparticles with zinc resulted in decreases in crystallite size from 24.23 nm to 21.15 nm, the lattice parameter from 8.45 A to 8.43 A and the coercivity from 41.20 Oe to 13.07 Oe. On the other hand, saturation magnetization increased from 50.12 emu/g to 57.36 emu/g following zinc doping. Image exposure analysis revealed that the reduction of MR signal intensity for zinc-doped magnetite nanoparticles was more than nondoped nanoparticles (shorter T2 relaxation time) thereby making the images darker.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"041002"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4029855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63489174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Application of Micropipette Aspiration in Molecular Mechanics of Single Cells.","authors":"Lap Man Lee,&nbsp;Allen P Liu","doi":"10.1115/1.4029936","DOIUrl":"https://doi.org/10.1115/1.4029936","url":null,"abstract":"<p><p>Micropipette aspiration is arguably the most classical technique in mechanical measurements and manipulations of single cells. Despite its simplicity, micropipette aspiration has been applied to a variety of experimental systems that span different length scales to study cell mechanics, nanoscale molecular mechanisms in single cells, bleb growth, and nucleus dynamics, to name a few. Enabled by micro/nanotechnology, several novel microfluidic devices have been developed recently with better accuracy, sensitivity, and throughput. Further technical advancements of microfluidics-based micropipette aspiration would have broad applications in both fundamental cell mechanics studies and for disease diagnostics.</p>","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 4","pages":"0408011-408016"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4029936","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34272098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Hydroxyapatite Nanoparticles on Biotransport Phenomena in Freezing HeLa Cells","authors":"Jingru Yi, Gang Zhao","doi":"10.1115/1.4029331","DOIUrl":"https://doi.org/10.1115/1.4029331","url":null,"abstract":"The effect of nanoparticles on subzero biotransport phenomena of living cells is very rare in the literature, although the information is of great importance for the application of nanotechnology in the field of cryobiology. In this study, subzero water transport phenomena in freezing HeLa cells in 1 × phosphate buffered saline (PBS) containing 0%, 0.05%, and 0.1% (w/w) hydroxyapatite (HA) nanoparticles with and without pre-incubation at 37 °C was quantitatively investigated. The results reveal that the presence of HA nanoparticles slightly facilitates the subzero water transport of HeLa cells.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"040904"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4029331","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63488343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Section on Nanoscale Materials, Devices, and Systems for Biosensing, Biomanipulation, and Biofabrication","authors":"Jianping Fu, D. Fan","doi":"10.1115/1.4030870","DOIUrl":"https://doi.org/10.1115/1.4030870","url":null,"abstract":"This special section of ASME Journal of Nanotechnology in Engineering and Medicine focuses on reporting state-of-the-art nanoscale materials, devices, and systems for advanced biosensing, biomanipulation, and biofabrication. Such nanoscale materials, devices, and systems can be organic, inorganic, and hybrid, and their applications for advanced biosensing, biomanipulation, and biofabrication have generated significant impact for important biology and biomedical applications. Nanotechnology has seen rapid progress in recent years, with advanced capabilities to generate and manipulate precisely engineered nanoscale organic and inorganic materials and their assemblies pointing toward the emergence of disruptive functionalities for diverse biological and biomedical applications. Furthermore, nanofabricated devices and systems such as nanofluidics, nanoelectromechanical systems, and nanophotonic structures with critical dimensions comparable to the molecular scale open up new possibilities for direct observation, manipulation, and analysis of biomolecules, thus providing a novel basis for ultrasensitive and high-resolution sensors and diagnostic systems. Nanoscale surface patterning tools for precisely controlling biomoleculeand cell-surface interactions and nanotools such as atomic force microscopy and optical and magnetic tweezers are also extremely powerful for controlling cell fate and function and studying molecular and cellular biomechanics. The following small but diverse selection of articles from different nanotechnology research areas describes current important topics of nanobiotechnology that we believe to be interesting, informative, and educational for the reader. An ongoing important research direction using nanotechnology is the sorting, enrichment, and informative analysis of rare cells from bodily fluids including circulating tumor cells (CTCs), antigen-specific T-cells, and hematopoietic stem cells. Highsensitivity sorting, detection, and analysis of such extremely rare cells can provide critical information for disease diagnosis and prognosis and advancing fundamental cellular understanding of physiological and pathological conditions. Qian et al. provide a concise review of the recent advance of using functional nanotopographic biomaterials for isolation of CTCs from blood specimens and their related nanofabrication methods. Qian et al. further discuss putative cellular mechanisms involving cell adhesion underlying the intrinsic nanotopography sensitive responses of CTCs. Another review contributed by Lee et al. provides a concise review of recent advances in micro/nanotechnology to improve the method of micropipette aspiration for applications in molecular and cellular biomechanics. Another emerging research area of nanotechnology is to develop synthetic nanostructured materials and surfaces for engineering control of cell-surface interactions and cell fate. In this issue, Han et al. report a method of generating single-crystalli","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"040201"},"PeriodicalIF":0.0,"publicationDate":"2014-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4030870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63490524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On Centrioles, Microtubules, and Cellular Electromagnetism","authors":"R. Huston","doi":"10.1115/1.4028855","DOIUrl":"https://doi.org/10.1115/1.4028855","url":null,"abstract":"This paper describes the inner workings of centrioles (a pair of small organelles adjacent to the nucleus) as they create cell electropolarity, engage in cell division (mitosis), but in going awry, also promote the development of cancers. The electropolarity arises from vibrations of microtubules composing the centrioles. Mitosis begins as each centrioles duplicates itself by growing a daughter centriole on its side. If during duplication more than one daughter is grown, cancer can occur and the cells divide uncontrollably. Cancer cells with supernumerary centrioles have high electropolarity which can serve as an attractor for charged therapeutic nanoparticles.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"031003"},"PeriodicalIF":0.0,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4028855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63487368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cohesive Zone Model for the Interface of Multiwalled Carbon Nanotubes and Copper: Molecular Dynamics Simulation","authors":"Ibrahim E Awad, L. Ladani","doi":"10.1115/1.4029462","DOIUrl":"https://doi.org/10.1115/1.4029462","url":null,"abstract":"Due to their superior mechanical and electrical properties, multiwalled carbon nano- tubes (MWCNTs) have the potential to be used in many nano-/micro-electronic applications, e.g., through silicon vias (TSVs), interconnects, transistors, etc. In particular, use of MWCNT bundles inside annular cylinders of copper (Cu) as TSV is proposed in this study. However, the significant difference in scale makes it difficult to evaluate the interfacial mechanical integrity. Cohesive zone models (CZM) are typically used at large scale to determine the mechanical adherence at the interface. However, at molecular level, no routine technique is available. Molecular dynamic (MD) simulations is used to determine the stresses that are required to separate MWCNTs from a copper slab and generate normal stress–displacement curves for CZM. Only van der Waals (vdW) interaction is considered for MWCNT/Cu interface. A displacement controlled loading was applied in a direction perpendicular to MWCNT’s axis in different cases with different number of walls and at different temperatures and CZM is obtained for each case. Fur- thermore, their effect on the CZM key parameters (normal cohesive strength ð r max Þ and the corresponding displacement ð d n Þ has been studied. By increasing the number of the walls of the MWCNT, r max was found to nonlinearly decrease. Displacement at maximum stress, d n , showed a nonlinear decrease as well with increasing the number of walls. Tem- perature effect on the stress–displacement curves was studied. When temperature was increased beyond 1 K, no relationship was found between the maximum normal stress and temperature. Likewise, the displacement at maximum load did not","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"031007"},"PeriodicalIF":0.0,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4029462","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63488562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 3,5,3′-Triiodothyroacetic Acid, Nanoencapsulated or Not, on Intact and Atrophic Skin in Rats","authors":"Daniele Trevizan Pera, Jéssica Freitas Planello, J. Cancino, I. Polikarpov, V. Zucolotto, L. Avó, C. M. R. Germano, D. G. Melo","doi":"10.1115/1.4028695","DOIUrl":"https://doi.org/10.1115/1.4028695","url":null,"abstract":"","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"5 1","pages":"031001"},"PeriodicalIF":0.0,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4028695","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63486821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}