Journal of nanotechnology in engineering and medicine最新文献

{"title":"Biofabrication of Multimaterial Three-Dimensional Constructs Embedded With Patterned Alginate Strands Encapsulating PC12 Neural Cell Lines","authors":"R. Dreher, B. Starly","doi":"10.1115/1.4031173","DOIUrl":"https://doi.org/10.1115/1.4031173","url":null,"abstract":"In this study, we report the bioprinting of a three-dimensional (3D) heterogeneous conduit structure encapsulating PC12 neural cells. A core–shell-based hybrid construct is fabricated by combining electrospinning, polymer extrusion, and cell-based bioprinting processes to create a multiscale and multimaterial conduit structure. PC12 nerve cells were shown to be printed with high cell viability (>95%) and to proliferate within the rolled construct at a rate consistent with traditional two-dimensional (2D) culture. Light microscopy and scanning electron microscopy (SEM) have also shown encapsulation of cells within the printed alginate gel and an even cell distribution throughout the heterogeneous cellular construct.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"021004"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaffold-Based or Scaffold-Free Bioprinting: Competing or Complementing Approaches?","authors":"I. Ozbolat","doi":"10.1115/1.4030414","DOIUrl":"https://doi.org/10.1115/1.4030414","url":null,"abstract":"Bioprinting is an emerging technology to fabricate artificial tissues and organs through additive manufacturing of living cells in a tissues-specific pattern by stacking them layer by layer. Two major approaches have been proposed in the literature: bioprinting cells in a scaffold matrix to support cell proliferation and growth, and bioprinting cells without using a scaffold structure. Despite great progress, particularly in scaffold-based approaches along with recent significant attempts, printing large-scale tissues and organs is still elusive. This paper demonstrates recent significant attempts in scaffold-based and scaffold-free tissue printing approaches, discusses the advantages and limitations of both approaches, and presents a conceptual framework for bioprinting of scale-up tissue by complementing the benefits of these approaches.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"024701"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4030414","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63489643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Suspended Hydrogel Membrane Platform for Cell Culture","authors":"Y. X. Chen, Shih-Ping Yang, Jiahan Yan, M. Hsieh, Lingyan Weng, Jessica L. Ouderkirk, M. Krendel, P. Soman","doi":"10.1115/1.4031467","DOIUrl":"https://doi.org/10.1115/1.4031467","url":null,"abstract":"Current cell-culture is largely performed on synthetic two-dimensional (2D) petri dishes or permeable supports such as Boyden chambers, mostly because of their ease of use and established protocols. It is generally accepted that modern cell biology research requires new physiologically relevant three-dimensional (3D) cell culture platform to mimic in vivo cell responses. To that end, we report the design and development of a suspended hydrogel membrane (ShyM) platform using gelatin methacrylate (GelMA) hydrogel. ShyM thickness (0.25–1 mm) and mechanical properties (10–70 kPa) can be varied by controlling the size of the supporting grid and concentration of GelMA prepolymer, respectively. GelMA ShyMs, with dual media exposure, were found to be compatible with both the cell-seeding and the cell-encapsulation approach as tested using murine 10T1/2 cells and demonstrated higher cellular spreading and proliferation as compared to flat GelMA unsuspended control. The utility of ShyM was also demonstrated using a case-study of invasion of cancer cells. ShyMs, similar to Boyden chambers, are compatible with standard well-plates designs and can be printed using commonly available 3D printers. In the future, ShyM can be potentially extended to variety of photosensitive hydrogels and cell types, to develop new in vitro assays to investigate complex cell–cell and cell–extracellular matrix (ECM) interactions.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"26 1","pages":"021002"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031467","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue on Three-Dimensional Bioprinting","authors":"I. Ozbolat","doi":"10.1115/1.4031391","DOIUrl":"https://doi.org/10.1115/1.4031391","url":null,"abstract":"","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"020301"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-Dimensional Printing Based Hybrid Manufacturing of Microfluidic Devices.","authors":"Yunus Alapan, Muhammad Noman Hasan, Richang Shen, Umut A Gurkan","doi":"10.1115/1.4031231","DOIUrl":"10.1115/1.4031231","url":null,"abstract":"<p><p>Microfluidic platforms offer revolutionary and practical solutions to challenging problems in biology and medicine. Even though traditional micro/nanofabrication technologies expedited the emergence of the microfluidics field, recent advances in advanced additive manufacturing hold significant potential for single-step, stand-alone microfluidic device fabrication. One such technology, which holds a significant promise for next generation microsystem fabrication is three-dimensional (3D) printing. Presently, building 3D printed stand-alone microfluidic devices with fully embedded microchannels for applications in biology and medicine has the following challenges: (i) limitations in achievable design complexity, (ii) need for a wider variety of transparent materials, (iii) limited z-resolution, (iv) absence of extremely smooth surface finish, and (v) limitations in precision fabrication of hollow and void sections with extremely high surface area to volume ratio. We developed a new way to fabricate stand-alone microfluidic devices with integrated manifolds and embedded microchannels by utilizing a 3D printing and laser micromachined lamination based hybrid manufacturing approach. In this new fabrication method, we exploit the minimized fabrication steps enabled by 3D printing, and reduced assembly complexities facilitated by laser micromachined lamination method. The new hybrid fabrication method enables key features for advanced microfluidic system architecture: (i) increased design complexity in 3D, (ii) improved control over microflow behavior in all three directions and in multiple layers, (iii) transverse multilayer flow and precisely integrated flow distribution, and (iv) enhanced transparency for high resolution imaging and analysis. Hybrid manufacturing approaches hold great potential in advancing microfluidic device fabrication in terms of standardization, fast production, and user-independent manufacturing.</p>","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976826/pdf/nihms-800846.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9395867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Creation of Highly Defined Mesenchymal Stem Cell Patterns in Three Dimensions by Laser-Assisted Bioprinting","authors":"E. Pagès, M. Rémy, V. Kériquel, M. Correa, B. Guillotin, F. Guillemot","doi":"10.1115/1.4031217","DOIUrl":"https://doi.org/10.1115/1.4031217","url":null,"abstract":"Bioprinting is a technology that allows making complex tissues from the bottom-up. The need to control accurately both the resolution of the printed droplet and the precision of its positioning was reported. Using a bioink with 1 × 108 cells/mL, we present evidence that the laser-assisted bioprinter (LAB) can deposit droplets of functional mesenchymal stem cells with a resolution of 138 ± 28 μm and a precision of 16 ± 13 μm. We demonstrate that this high printing definition is maintained in three dimensions.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"021006"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031217","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid Tissue Engineering Scaffolds by Combination of Three-Dimensional Printing and Cell Photoencapsulation.","authors":"Marica Markovic, Jasper Van Hoorick, Katja Hölzl, Maximilian Tromayer, Peter Gruber, Sylvia Nürnberger, Peter Dubruel, Sandra Van Vlierberghe, Robert Liska, Aleksandr Ovsianikov","doi":"10.1115/1.4031466","DOIUrl":"10.1115/1.4031466","url":null,"abstract":"<p><p>Three-dimensional (3D) printing offers versatile possibilities for adapting the structural parameters of tissue engineering scaffolds. However, it is also essential to develop procedures allowing efficient cell seeding independent of scaffold geometry and pore size. The aim of this study was to establish a method for seeding the scaffolds using photopolymerizable cell-laden hydrogels. The latter facilitates convenient preparation, and handling of cell suspension, while distributing the hydrogel precursor throughout the pores, before it is cross-linked with light. In addition, encapsulation of living cells within hydrogels can produce constructs with high initial cell loading and intimate cell-matrix contact, similar to that of the natural extra-cellular matrix (ECM). Three dimensional scaffolds were produced from poly(lactic) acid (PLA) by means of fused deposition modeling. A solution of methacrylamide-modified gelatin (Gel-MOD) in cell culture medium containing photoinitiator Li-TPO-L was used as a hydrogel precursor. Being an enzymatically degradable derivative of natural collagen, gelatin-based matrices are biomimetic and potentially support the process of cell-induced remodeling. Preosteoblast cells MC3T3-E1 at a density of 10 × 10⁶ cells per 1 mL were used for testing the seeding procedure and cell proliferation studies. Obtained results indicate that produced constructs support cell survival and proliferation over extended duration of our experiment. The established two-step approach for scaffold seeding with the cells is simple, rapid, and is shown to be highly reproducible. Furthermore, it enables precise control of the initial cell density, while yielding their uniform distribution throughout the scaffold. Such hybrid tissue engineering constructs merge the advantages of rigid 3D printed constructs with the soft hydrogel matrix, potentially mimicking the process of ECM remodeling.</p>","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 2 1","pages":"0210011-210017"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave Properties of Nanocomposites: Effect of Manufacturing Methods and Nanofiller Structure","authors":"A. A. Khurram, S. A. Rakha, N. Ali, I. Gul, A. Munir","doi":"10.1115/1.4029916","DOIUrl":"https://doi.org/10.1115/1.4029916","url":null,"abstract":"Nanocomposite materials filled with multiwall carbon nanotubes (MWCNTs) having three types of structures, i.e., longer (200lm), shorter (20‐50lm), and aminated (20‐50lm), are manufactured for microwave absorption (MA) in 11‐17GHz frequency range. Microstructure, dielectric permittivity, direct current (DC) electrical conductivity, and MA properties of the MWCNTs‐epoxy nanocomposite were investigated. A correlation has been developed between the structure (aspect ratio and surface functionality) of MWCNTs, electrical conductivity of the composite, and MA (return loss (RL)). E-glass/epoxy composite filled with longer carbon nanotubes (CNTs) has shown higher RL as compared to that of other two nanocomposites. The measurements have shown that the magnitude of RL of microwaves depends strongly on the structure of MWCNTs used in the composite. Furthermore, the effect of synthesis route followed for the manufacturing of nanocomposite on its electrical conductivity and microwave absorbing properties is also investigated; three different approaches were followed to manufacture CNT/epoxy nanocomposites from longer CNTs (200lm). [DOI: 10.1115/1.4029916]","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"014501"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4029916","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63489045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanostructured Aluminum Oxide Black Coating: Electrochemical, Mechanical, and Optical Characterizations","authors":"A. Awad, M. Shaffei, H. Hussein","doi":"10.1115/1.4030920","DOIUrl":"https://doi.org/10.1115/1.4030920","url":null,"abstract":"","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"011002"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4030920","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63490678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visualization and Motion of Curcumin Loaded Iron Oxide Nanoparticles During Magnetic Drug Targeting","authors":"M. Asfer, A. P. Prajapati, Arun Kumar, P. Panigrahi","doi":"10.1115/1.4031062","DOIUrl":"https://doi.org/10.1115/1.4031062","url":null,"abstract":"Magnetic drug targeting (MDT) involves the localization of drug loaded iron oxide nanoparticles (IONPs) around the malignant tissue using external magnetic field for therapeutic purposes. The present in vitro study reports the visualization and motion of curcumin loaded IONPs (CU-IONPs) around the target site inside a microcapillary (500 × 500 μm2 square cross section), in the presence of an externally applied magnetic field. Application of magnetic field leads to transportation and aggregation of CU-IONPs toward the target site inside the capillary adjacent to the magnet. The localization/aggregation of CU-IONPs at the target site shows strong dependence on the strength of the applied magnetic field and flow rate of ferrofluid through the capillary. Such an in vitro study offers a viable for optimization and design of MDT systems for in vivo applications.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"011004"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}