Yuhui Li, Xiang Li, Lu Zheng, Lin Wang, Xiaohui Zhang, Feng Xu
{"title":"Polyacrylamide/GelMA Hydrogel Templates for Breast Cancer Cell Spheroids Fabrication","authors":"Yuhui Li, Xiang Li, Lu Zheng, Lin Wang, Xiaohui Zhang, Feng Xu","doi":"10.1115/1.4031898","DOIUrl":"https://doi.org/10.1115/1.4031898","url":null,"abstract":"Fabrication of cellular spheroids is critical for creating functional tissue units and investigating the mechanism of tumorigenesis, development, and intercellular and cell–matrix interactions in vitro. Herein, we developed a novel, simple, and facile method for cell spheroid fabrication by using polyacrylamide/gelatin methacrylate (PA/GelMA) hydrogel composites. Arrays of Michigan Cancer Foundation-7 (MCF-7) breast cancer cell spheroids can be easily formed by tuning the GelMA composition. The shape and size of cell spheroids can be also well controlled by regulating cell seeding density and culturing time. All these results suggested that this simple and facile platform can serve as a useful tool to generate three-dimensional (3D) cell spheroids and can be integrated within high-throughput drug screening platforms, which will be of great help in engineering functional tissue models and regenerative medicines.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"034501"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031898","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63493320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Discussion: “A Review and Parametric Investigation Into Nanofluid Viscosity Models” (Nwosu, P. N., Meyer, J., and Sharifpur, M., 2014, ASME J. Nanotechnol. Eng. Med., 5(3), p. 031008)","authors":"M. Awad","doi":"10.1115/1.4032016","DOIUrl":"https://doi.org/10.1115/1.4032016","url":null,"abstract":"","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"035501"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4032016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63493240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Three-Dimensional Reconstruction of Blood Vessels of the Human Retina by Fractal Interpolation.","authors":"Hichem Guedri, Jihen Malek, Hafedh Belmabrouk","doi":"10.1115/1.4032170","DOIUrl":"https://doi.org/10.1115/1.4032170","url":null,"abstract":"In this work, data from two-dimensional (2D) images of the human retina were taken as a case study. First, the characteristic data points had been removed using the Douglas-Peucker (DP) method, and subsequently, more data points were added using random fractal interpolation approach, to reconstruct a three-dimensional (3D) model of the blood vessel. By visualizing the result, we can see that all the small blood vessels in the human retina are more visible and detailed. This algorithm of 3D reconstruction has the advantage of being fast with calculation time less than 40 s and also can reduce the 3D image storage level on a disk with a reduction ratio between 78% and 96.65%.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 3","pages":"0310031-310035"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4032170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34515531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vibrations of Fractal Structures: On the Nonlinearities of Damping by Branching","authors":"P. Torab, D. Piovesan","doi":"10.1115/1.4032224","DOIUrl":"https://doi.org/10.1115/1.4032224","url":null,"abstract":"To study the effect of damping due to branching in trees and fractal structures, a harmonic analysis was performed on a finite element model using commercially available software. The model represented a three-dimensional (3D) fractal treelike structure, with properties based on oak wood and with several branch configurations. As branches were added to the model using a recursive algorithm, the effects of damping due to branching became apparent: the first natural frequency amplitude decreased, the first peak widened, and the natural frequency decreased, whereas higher frequency oscillations remained mostly unaltered. To explain this nonlinear effect observable in the spectra of branched structures, an analytical interpretation of the damping was proposed. The analytical model pointed out the dependency of Cartesian damping from the Coriolis forces and their derivative with respect to the angular velocity of each branch. The results provide some insight on the control of chaotic systems. Adding branches can be an effective way to dampen slender structures but is most effective for large deformation of the structure.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"034502"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4032224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63494354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Merdji, N. Della, Ali Benaissa, B. B. Bouiadjra, B. Serier, R. Mootanah, I. Muslih, O. Mukdadi
{"title":"Numerical Analysis of Dental Caries Effect on the Biomechanical Behavior of the Periodontal System","authors":"A. Merdji, N. Della, Ali Benaissa, B. B. Bouiadjra, B. Serier, R. Mootanah, I. Muslih, O. Mukdadi","doi":"10.1115/1.4032689","DOIUrl":"https://doi.org/10.1115/1.4032689","url":null,"abstract":"The aim of this study was to investigate the effect of dental caries on the stability of the periodontal system. This study presents a numerical analysis performed with three-dimensional (3D) finite element (FE) method to evaluate stresses in the bone surrounding the tooth with dynamic mastication combined loadings. In this work, we present a comparative study on infected and healthy periodontal systems. The infected tooth was modeled and a caries defect was introduced to the tooth coronal part. The infected tooth was evaluated and equivalent von Mises interface stress values were obtained for comparison with the ones exhibited by the healthy tooth. Our results by 3D FE analysis indicated that maximum stresses occurred primarily at the cervical level of root and alveolar bone. In the cortical bone, the stress value was greater in infected system (21.641 MPa) than in healthy system (15.752 MPa), i.e., a 37.4% increase. However, in the trabecular bone we observed only 1.6% increase in the equivalent stress values for the infected tooth model. Stress concentration at the cervical level may cause abnormal bone remodeling or bone loss, resulting loss of tooth attachment or bone damage. Our findings showed that decayed single-rooted teeth are more vulnerable to apical root resorption than healthy teeth. The numerical method presented in this study not only can aid the elucidation of the biomechanics of teeth infected by caries but also can be implemented to investigate the effectiveness of new advanced restorative materials and protocols.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"031004"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4032689","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63494950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analyzing Origin of Multifractality of Surface Electromyography Signals in Dynamic Contractions","authors":"K. Marri, R. Swaminathan","doi":"10.1115/1.4032005","DOIUrl":"https://doi.org/10.1115/1.4032005","url":null,"abstract":"The aim of this study is analyze the origin of multifractality of surface electromyography (sEMG) signals during dynamic contraction in nonfatigue and fatigue conditions. sEMG signals are recorded from triceps brachii muscles of twenty two normal healthy subjects. The signals are divided into six equal segments on time scale for normalization. The first and sixth segments are considered as nonfatigue and fatigue condition respectively. The source of multifractality can be due to correlation and probability distribution. The original sEMG series are transformed into shuffled and surrogate series. These three series namely, original, shuffled and surrogate series in nonfatigue and fatigue conditions are subjected to multifractal detrended fluctuation analysis (MFDFA) and features are extracted. The results indicate that sEMG signals exhibit multifractal behavior. Further investigation revealed that origin of multifractality is primarily due to correlation. The origin of multifractality due to correlation is quantified as 80% in nonfatigue and 86% in fatigue conditions. This method of multifractal analysis may be useful for analyzing progressive changes in muscle contraction in varied neuromuscular studies.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"031002"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4032005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63493147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Constantine C. Doumanidis, I. Gunduz, C. Rebholz, C. Doumanidis
{"title":"Real-Time Computational Model of Ball-Milled Fractal Structures","authors":"Constantine C. Doumanidis, I. Gunduz, C. Rebholz, C. Doumanidis","doi":"10.1115/1.4031276","DOIUrl":"https://doi.org/10.1115/1.4031276","url":null,"abstract":"","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"1141 1","pages":"031001"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031276","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Campbell, Ian McGuinness, H. Wirz, A. Sharon, A. Sauer-Budge
{"title":"Multimaterial and Multiscale Three-Dimensional Bioprinter","authors":"J. Campbell, Ian McGuinness, H. Wirz, A. Sharon, A. Sauer-Budge","doi":"10.1115/1.4031230","DOIUrl":"https://doi.org/10.1115/1.4031230","url":null,"abstract":"","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"16 36 1","pages":"021005"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031230","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Narayanan, Arun Kumar, Zhuo Tan, S. Bernacki, B. Starly, Rohan A. Shirwaiker
{"title":"Alginate Microspheroid Encapsulation and Delivery of MG-63 Cells Into Polycaprolactone Scaffolds: A New Biofabrication Approach for Tissue Engineering Constructs","authors":"L. Narayanan, Arun Kumar, Zhuo Tan, S. Bernacki, B. Starly, Rohan A. Shirwaiker","doi":"10.1115/1.4031174","DOIUrl":"https://doi.org/10.1115/1.4031174","url":null,"abstract":"Scaffolds play an important role in tissue engineering by providing structural framework and a surface for cells to attach, proliferate, and secrete extracellular matrix (ECM). In order to enable efficient tissue formation, delivering sufficient cells into the scaffold three-dimensional (3D) matrix using traditional static and dynamic seeding methods continues to be a critical challenge. In this study, we investigate a new cell delivery approach utilizing deposition of hydrogel-cell encapsulated microspheroids into polycaprolactone (PCL) scaffolds to improve the seeding efficiency. Three-dimensional-bioplotted PCL constructs (0 deg/90 deg lay down, 284 ± 6 μm strand width, and 555 ± 8 μm strand separation) inoculated with MG-63 model bone cells encapsulated within electrostatically generated calcium-alginate microspheroids (O 405 ± 13 μm) were evaluated over seven days in static culture. The microspheroids were observed to be uniformly distributed throughout the PCL scaffold cross section. Encapsulated cells remained viable within the constructs over the test interval with the highest proliferation noted at day 4. This study demonstrates the feasibility of the new approach and highlights the role and critical challenges to be addressed to successfully utilize 3D-bioprinting for microencapsulated cell delivery.","PeriodicalId":73845,"journal":{"name":"Journal of nanotechnology in engineering and medicine","volume":"6 1","pages":"021003"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1115/1.4031174","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63491112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}