Journal of microorganism control最新文献_第3页

Correlation between disinfection efficacy and cumulative amount of free chlorine reaching various positions during ultrasonic fogging with hypochlorite solution. 次氯酸盐溶液超声波雾化过程中，消毒效果与到达不同位置的游离氯累积量之间的相关性。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.2_75

Satoshi Fukuzaki, Hajime Hotta, Shun Nojima

{"title":"Correlation between disinfection efficacy and cumulative amount of free chlorine reaching various positions during ultrasonic fogging with hypochlorite solution.","authors":"Satoshi Fukuzaki, Hajime Hotta, Shun Nojima","doi":"10.4265/jmc.29.2_75","DOIUrl":"https://doi.org/10.4265/jmc.29.2_75","url":null,"abstract":"When a hypochlorite solution is ultrasonically fogged in a room, free chlorine, i.e., HOCl and OCl-, reaches various positions in two forms: fine fog droplets and gaseous hypochlorous acid（HOCl(g)）. In this study, the cumulative amount of free chlorine reaching various positions on the floor away from the fogger was measured in a 90-m3 room, using a sulfamate-carrying glass-fiber filter indicator. The fine droplets were blown out from the fogger into the spaces at different discharge port angles of 30 - 90°. Free chlorine was successfully trapped by sulfamate, forming monochlorosulfamate, which was stably retained on the indicator. The cumulative amount of free chlorine（ ng/indicator） increased with fogging time at each position and depended on the blow angle and distance from the fogger. Minor differences in the HOCl(g) concentration near the floor at all positions were observed. The disinfection efficacy of the fogging treatment against Staphylococcus aureus on wet surfaces was relatively higher at positions near the fogger and lower at positions far from the fogger. At each discharge port angle, a strong correlation between the logarithmic reduction in relative viable cells and the cumulative amount of free chlorine reaching S. aureus plates was observed. The slopes of the regression lines of correlation diagrams as a function of the cumulative amount of free chlorine were between -0.0362 and -0.0413 ng-1. This study demonstrated that the cumulative amount of free chlorine measured using the filter indicator could reflect the sum of the free chlorine of both fine droplets and HOCl(g), and that the disinfection efficiency depended on the cumulative amount of free chlorine reaching different areas.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 2","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141332656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems. 在实验室模型系统中吸入气态次氯酸及其对人类呼吸道上皮细胞的影响。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.1_39

Takashi Muramatsu, Kazuya Kodama, Tetsuyoshi Yamada, Atsushi Yamada, Satoshi Fukuzaki

{"title":"Inhalation of gaseous hypochlorous acid and its effect on human respiratory epithelial cells in laboratory model systems.","authors":"Takashi Muramatsu, Kazuya Kodama, Tetsuyoshi Yamada, Atsushi Yamada, Satoshi Fukuzaki","doi":"10.4265/jmc.29.1_39","DOIUrl":"10.4265/jmc.29.1_39","url":null,"abstract":"During the disinfection of indoor spaces using gaseous hypochlorous acid (HOCl(g)), inhalation is the most common route of exposure for humans. In this study, an artificial human respiratory tract model was exposed to 12-140 ppb HOCl(g) at an aspiration flow rate of 800 mL/s for 15 h in a 1 m3 chamber. The respiratory tract model was equipped with 5th order bronchi and all gas-contact parts were made of silicone rubber with no other chlorine-consuming substances. The concentration of HOCl(g) reaching the lung pseudo-space was approximately 47.4% of the HOCl(g) concentrations in the chamber and was calculated to be very close to zero when the chamber concentration was less than 20.5 ppb. The disappearance of HOCl(g) during inhalation is likely due to the adsorption of HOCl(g) on the gas-contact silicone rubber surfaces. The cytotoxicity of HOCl(g) on respiratory epithelial cells was also examined using human air-liquid-interface airway tissue models. Human nasal epithelium and bronchiolar epithelium were exposed to 100 ppb and 500 ppb HOCl(g) for 8 h and 5 d, respectively. No significant effects of HOCl(g) on cell viability and ciliary activity were observed in any cell type, indicating that low concentrations of HOCl(g）, less than 500 ppb, had no cytotoxic effect.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 1","pages":"39-44"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species diversity of xerophilic Aspergillus and Penicillium in marine surface waters revealed by isolation using osmophilic medium. 通过使用嗜渗透培养基进行分离，揭示海洋表层水域嗜干旱曲霉和青霉的物种多样性。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.1_17

Ryo Hagiuda, Dai Hirose

{"title":"Species diversity of xerophilic Aspergillus and Penicillium in marine surface waters revealed by isolation using osmophilic medium.","authors":"Ryo Hagiuda, Dai Hirose","doi":"10.4265/jmc.29.1_17","DOIUrl":"10.4265/jmc.29.1_17","url":null,"abstract":"The species diversity of xerophilic and halophilic fungi distributed in marine surface water was studied at four local sites located in two geographically distant regions in Japan. At each site, 5-10 samples were collected and isolated using an osmophilic medium. Species identification was conducted based on nucleotide sequence of calmodulin or β -tubulin and morphological characteristics for Aspergillus, Penicillium, and Talaromyces, and on the sequences of rRNA internal transcribed spacer for the other taxa. Overall, 231 strains were isolated from all sites and classified into 85 species belonged to 12 orders and 33 genera. The isolates that showed better mycelial growth than the control（no NaCl added) in the halotolerance test were defined as halophilic fungi, and only 22 species（10 Aspergillus species and 12 Penicillium species) were halophilic. Comparison of the halophilic fungal flora of the two regions revealed that four species common to both regions were isolated for Aspergillus, but no such species were isolated for Penicillium. Given that 15 halophilic species（10 Aspergillus and 5 Penicillium species) are known to be xerophilic species distributed in indoor environments, it can be inferred that indoor xerophilic species are likely to be widely distributed in marine surface water.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 1","pages":"17-26"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of antibacterial and antiviral efficacy of volatile organic compounds. 评估挥发性有机化合物的抗菌和抗病毒功效。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.3_113

Shoko Hamada, Mitsuhiro Gomi

引用次数: 0

Distribution of Staphylococcus aureus carriage among healthcare workers in a Japanese convalescent and rehabilitation hospital. 日本一家疗养康复医院医护人员携带金黄色葡萄球菌的分布情况。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.1_33

Akiko Kanayama Katsuse, Izumo Kanesaka, Misato Enomoto, Satoko Tanikawa, Hiroshi Takahashi, Miyuki Nagano, Intetsu Kobayashi

{"title":"Distribution of Staphylococcus aureus carriage among healthcare workers in a Japanese convalescent and rehabilitation hospital.","authors":"Akiko Kanayama Katsuse, Izumo Kanesaka, Misato Enomoto, Satoko Tanikawa, Hiroshi Takahashi, Miyuki Nagano, Intetsu Kobayashi","doi":"10.4265/jmc.29.1_33","DOIUrl":"10.4265/jmc.29.1_33","url":null,"abstract":"Earlobes, nasal cavities, and fingers of 145 healthcare workers in convalescent and rehabilitation hospital (60 nurses and 85 rehabilitation healthcare workers) were sampled. Of the 3 sites sampled, Staphylococcus aureus was detected in one or more sites in 25 nurses and 27 rehabilitation workers. S. aureus was detected in all 3 sites in 2 (8.0%) nurses and 2 (7.4%) rehabilitation workers, and the S. aureus isolates in each case showed related PFGE pattern. S. aureus was detected in both the fingers and nasal cavities of 5 (18.5%) of the rehabilitation healthcare workers; in all 5 cases, the PFGE patterns of the S. aureus isolates from each site belonged to same cluster based on PFGE. Of the 2 cases in which methicillinresistant S. aureus (MRSA) was recovered from earlobes, fingers, and nasal cavities, in both cases, MRSA isolates from the 3 sites were the same clone according to PFGE analysis and SCCmec type IV. As S. aureus was detected in pierced earlobes of nurses, hand hygiene must be practiced after touching pierced earlobes and before patient contact. The same S. aureus clone in the nasal cavity and earlobes indicates that the route of transmission is through the fingers.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 1","pages":"33-37"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergy analysis with extended isobolography and its conceptual basis for the evaluation of antimicrobial effects of combined physical and chemical treatments. 物理和化学联合处理抗菌效果评价的扩展等密度协同分析及其概念基础。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.1_35

Tetsuaki Tsuchido

引用次数: 0

Bacteriophage PVN06 protected catfish Pangasianodon hypophthalmus from Edwardsiella ictaluri infection. 噬菌体PVN06保护下眼鲶鱼免受爱德华氏菌感染。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.2_57

To H Ngoc, Dang T H Oanh, LE T M Duyen, Tran T T Xuan, Hoang A Hoang, LE P Nga

{"title":"Bacteriophage PVN06 protected catfish Pangasianodon hypophthalmus from Edwardsiella ictaluri infection.","authors":"To H Ngoc, Dang T H Oanh, LE T M Duyen, Tran T T Xuan, Hoang A Hoang, LE P Nga","doi":"10.4265/jmc.28.2_57","DOIUrl":"https://doi.org/10.4265/jmc.28.2_57","url":null,"abstract":"Bacillary necrosis of pangasius (BNP) is a disease caused by Edwardsiella ictaluri bacteria in striped catfish Pangasianodon hypophthalmus that results in high mortality rates. To control this disease, bacteriophages have been considered as alternatives to antibiotics. In this study, we applied the lytic bacteriophage PVN06 in striped catfish fingerlings to prevent E. ictaluri infection. In an experimental trial, the phage was administered to fish by feeding phage-coated feed with doses of 7.17±0.09, 8.17±0.09 and 9.17±0.09 log PFU/g feed per day before bacterial infection. Fish were infected by bacteria once with concentrations ranging from 3.01 to 7.01 log CFU/ml tank water. A day after infection, phage treatment resumed at a rate of once per day until the end of the trial. The results of the trial show that bacterial infection caused typical symptoms of BNP in fish with the cumulative fish death rate of 36.7±2.9 to 75.0±5.0%, depending on the bacterial concentration used for infection. Phage treatment with 9.17±0.09 log PFU/g significantly reduced the mortality rate, while treatments with 8.17±0.09 and 7.17±0.09 log PFU/g did not. This phage dose resulted in a 61.7-fold reduction in the toxicity of the bacterial pathogen and the survival rate of 15-23.3% in fish. Our study has demonstrated that the bacteriophage PVN06 protected striped catfish from BNP.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 2","pages":"57-64"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10120751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N -acylhomoserine lactone-degrading activity of Trichoderma species and its application in the inhibition of bacterial quorum sensing. 木霉对N-酰基高丝氨酸内酯的降解活性及其在抑制细菌群体感应中的应用。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_139

Tomohiro Morohoshi, Waka Arai, Nobutaka Someya

引用次数: 0

Viability of sublethally injured indicator and pathogenic coliform bacteria on fresh-cut cabbage during storage in an active MAP of 10% CO₂. 鲜切卷心菜在10%CO2的活性MAP中储存期间，亚致死损伤指标和致病大肠菌群的存活率。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_109

Kiyoshi Sato, Hidemi Izumi

{"title":"Viability of sublethally injured indicator and pathogenic coliform bacteria on fresh-cut cabbage during storage in an active MAP of 10% CO2.","authors":"Kiyoshi Sato, Hidemi Izumi","doi":"10.4265/jmc.28.3_109","DOIUrl":"10.4265/jmc.28.3_109","url":null,"abstract":"Shredded cabbage treated with either tap water or electrolyzed water was stored in an active modified atmosphere packaging (MAP) of 10% CO2 for 5 d at 10℃, 7 d at 5℃, and 8 d at 1℃ to evaluate the occurrence and viability of sublethally injured coliform bacteria. The CO2 and O2 concentrations in the packages approached an equilibrium of 10% CO2 and 10% O2 during storage at all temperatures tested. Coliforms in shredded cabbage increased during storage at all three temperatures, with the increase being greater at 10℃. Sublethal injury at 65% to 69% for the coliforms was detected only on cabbage samples treated with electrolyzed water and stored at 5℃ for 4 and 7 d. Enterobacter cloacae was one of the injured species of coliform bacteria in shredded cabbage. Shredded cabbage was inoculated with chlorine-injured Escherichia coli O157:H7 and stored at 5℃ for 6 d in an active MAP of 10% CO2. Counts of E. coli O157:H7 remained almost constant during storage, and injured E. coli O157:H7 ranging from 50% to 70% were found on shredded cabbage throughout the storage period. These results indicate that sublethally injured indicator and pathogenic bacteria would be found on fresh-cut cabbage in the realistic MAP storage at 5℃.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"109-116"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of sodium chlorite in inactivating Vibrio parahaemolyticus attached to polyethylene terephthalate surfaces. 亚氯酸钠对附着在聚对苯二甲酸乙二醇酯表面的副溶血性弧菌的灭活效果。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_135

Kazuhiro Takahashi, Satoshi Fukuzaki

引用次数: 0