Journal of microorganism control最新文献

Development of an additive for simple enhanced selectivity of selective agar plates for the detection of Legionella. 一种用于军团菌检测的简单增强选择性琼脂板添加剂的研制。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.3_103

Hiroaki Inoue, Manami Kitazume, Yukie Saito

引用次数: 0

Bacterial flora analyses and isolation of adhesive bacteria on fabrics washed by home laundry. 家用洗衣机洗涤织物上粘附细菌的菌群分析与分离。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.1_1

Hiroaki Okuda, Hirofumi Noda, Mugihei Ikemizu, Katsuya Wakita, Yoshinobu Matsumura

{"title":"Bacterial flora analyses and isolation of adhesive bacteria on fabrics washed by home laundry.","authors":"Hiroaki Okuda, Hirofumi Noda, Mugihei Ikemizu, Katsuya Wakita, Yoshinobu Matsumura","doi":"10.4265/jmc.30.1_1","DOIUrl":"10.4265/jmc.30.1_1","url":null,"abstract":"In this report, microbial cells on washed fabrics and in washing water in home laundry were analyzed, in order to suppress malodors and construct a highly sanitary home laundry system. Many bacterial cells (109-1010 cells/g dry fabric) were detected from washed fabrics emitting relative strong malodors even after washing. Detergents with or without antimicrobial agents partially removed the microorganism cells and more than 20% of cells survived even after washing and drying in almost all households. Bacterial flora analyses of washed fabrics revealed the presence of several typical genera: Enhydrobacter sp., Paracoccus sp., Sphingomonas sp., Roseomonas sp. and Kocuria sp. These results indicated that it is difficult to remove or eliminate microbial cells adhering to clothes and fabrics in home laundry. This report also indicated certain typical bacteria (adhesive type) seem to adhere or attach to fabrics, while bacteria (transient type) found in washing water have low adhesivity to fabrics. Therefore, understanding the adhesive properties of bacteria on fabrics may permit more efficient removal of microorganisms and reduction of malodors in home laundry.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of a Membrane Filter Protocol for Direct Identification of Microorganisms in Dog Ear Discharge using MALDI-TOF MS. MALDI-TOF质谱法直接鉴定犬耳分泌物微生物的膜过滤方案研究。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.2_33

Hiroto Maeda, Miki Sawatari, Momori Shimizu, Takayuki Ishige, Toshibumi Taniguchi, Toshifumi Watanabe, Kazuyuki Sogawa

{"title":"Investigation of a Membrane Filter Protocol for Direct Identification of Microorganisms in Dog Ear Discharge using MALDI-TOF MS.","authors":"Hiroto Maeda, Miki Sawatari, Momori Shimizu, Takayuki Ishige, Toshibumi Taniguchi, Toshifumi Watanabe, Kazuyuki Sogawa","doi":"10.4265/jmc.30.2_33","DOIUrl":"https://doi.org/10.4265/jmc.30.2_33","url":null,"abstract":"Otitis externa is an ear disease that is present in 10-20% of dogs. Bacterial organisms are a common cause, and infection may cause severe and chronic inflammation. Thus, there is a need for early detection and identification of bacteria in ear discharge samples. In this study, 31 such samples were analyzed to identify microorganisms using MALDI-TOF MS with pretreatment using a membrane filtration protocol and a rapid BACpro II kit, compared to pretreatment with a rapid BACpro II kit alone. Reliable scores for E. coli and E. faecalis were obtained after inoculation of samples with 1.0×104 CFU/ml using the membrane filter with the rapid BACpro II kit, with approximately 10-fold higher sensitivity compared to rapid BACpro pretreatment alone. Among 31 bacteria-positive colonies in the samples, MALDI-TOF MS and membrane filtration (0.80 µm) with a rapid BACpro II kit was significantly more accurate compared to use of the kit alone (29 (82.9%) vs. 17 (48.6%) isolates identified, p<0.001). These results show that MALDI-TOF MS with a membrane filter and a rapid BACpro II kit is a quick and reliable method for bacterial identification in ear discharge samples.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 2","pages":"33-39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bactericidal Action of Ethanol-soluble Lignin from Steam-exploded Rice Straw Combined with Ultraviolet-A Irradiation. 蒸爆稻草乙醇溶木质素与紫外- a联合辐照的杀菌作用。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.3_81

Akihiro Shirai, Akihiro Suzuki, Yoshitoshi Nakamura, Chikako Asada

{"title":"Bactericidal Action of Ethanol-soluble Lignin from Steam-exploded Rice Straw Combined with Ultraviolet-A Irradiation.","authors":"Akihiro Shirai, Akihiro Suzuki, Yoshitoshi Nakamura, Chikako Asada","doi":"10.4265/jmc.30.3_81","DOIUrl":"https://doi.org/10.4265/jmc.30.3_81","url":null,"abstract":"This study aimed to investigate the bactericidal effect of ethanol-soluble lignin (ESL) from rice straw under ultraviolet-A (UV-A) irradiation. Following steam explosion pretreatment of rice straw, ethanol extraction was used to separate the lignin. The total phenolic content (TPC) in the ESL was 258 mg/g (gallic acid equivalent). Under UV-A irradiation, ESL exhibited bactericidal activity against Gram-positive and -negative bacteria at ≤10 mg TPC/L. Fluorescence experiments demonstrated significant adsorption of ESL to the cell surface of Staphylococcus aureus. Scanning electron microscopy revealed membrane deformation, producing many vesicles on the cell surface, when the ESL was activated by UV-A irradiation. These morphological changes were associated with an increase in membrane permeability, which elevated cell counts stained with propidium iodide to 51% and resulted in a 57% decrease in the intracellular ATP level. ESL exposed to UV-A light generated hydrogen peroxide. Addition of a scavenger of reactive oxygen species (ROS) or replacement of dissolved oxygen with argon gas in a bacterial suspension significantly suppressed the synergistic bactericidal activity of ESL and UV-A radiation, indicating that ROS are involved in the bactericidal mechanism. Organosolv lignin pretreated using steam explosion can be developed for antimicrobial application in combination with UV-A irradiation.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 3","pages":"81-88"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple Detection of Culturable Legionella pneumophila in Cooling Tower Water Samples using Legiolert. 用Legiolert对冷却塔水样中可培养嗜肺军团菌的简单检测。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.3_99

Hiroaki Inoue, Masaki Okamoto, Miyuki Katada, Tatsuyuki Kaneko, Masato Sensui, Hiroaki Nakata, Hironori Hattori

引用次数: 0

A theoretical model for evaluating a specific mode of lethal injury that causes reproductive death in an irradiated microbial population. 一个理论模型，用于评估一种特定模式的致命伤害，导致生殖死亡的辐照微生物群体。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.2_55

Tetsuaki Tsuchido, Khanh C Vo, Ryoko Asada, Masakazu Furuta

引用次数: 0

Development of an Alternative to Preservation Efficacy Tests Using the Metabolic Activity of Microorganisms. 利用微生物代谢活性进行保鲜效果试验的替代方法的发展。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.3_71

Mai Watanabe, Asako Mitani, Jun Sato, Takumi Sonoda, Naofumi Shigemune

{"title":"Development of an Alternative to Preservation Efficacy Tests Using the Metabolic Activity of Microorganisms.","authors":"Mai Watanabe, Asako Mitani, Jun Sato, Takumi Sonoda, Naofumi Shigemune","doi":"10.4265/jmc.30.3_71","DOIUrl":"https://doi.org/10.4265/jmc.30.3_71","url":null,"abstract":"Consumer products such as detergents, fabric softeners, and cosmetics are typically used in various environments. Manufacturers must ensure the quality of products and ensure their safety through their lifecycle, from manufacturing to usage. Preservation efficacy testing (PET) is typically conducted to determine the preservative of a product prior to use. However, PET is time-consuming and laborious due to its long-term storage and culture-based detection methods. In this study, we focused on the metabolic activities of microorganisms and developed a rapid alternative to PET. We observed that 72 h of preservation was sufficient to predict preservation efficacy using the metabolic activity method. Finally, the accuracy of the metabolic activity method was compared to that of PET using a variety of products such as detergents and cosmetics. The accuracy of this alternative method exceeds 85% for highly fluid and easy-to-handle samples. These results suggest that the developed metabolic activity method possesses the potential to determine the preservative efficacy of products in only four days（72 h of preservation and 24 h of detection）. Thus, this method possesses the potential to provide a rapid and simple alternative to PET.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 3","pages":"71-79"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatilization of gaseous hypochlorous acid from calcium hypochlorite product induced by the deliquescent property of calcium chloride. 氯化钙潮解特性引起的次氯酸钙产品气态次氯酸的挥发。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.1_13

Soshi Omura, Hideyuki Seki, Mami Ishikawa, Satoshi Fukuzaki

{"title":"Volatilization of gaseous hypochlorous acid from calcium hypochlorite product induced by the deliquescent property of calcium chloride.","authors":"Soshi Omura, Hideyuki Seki, Mami Ishikawa, Satoshi Fukuzaki","doi":"10.4265/jmc.30.1_13","DOIUrl":"10.4265/jmc.30.1_13","url":null,"abstract":"The commercial products of calcium hypochlorite (Ca（OCl) 2) , a solid hypochlorite salt, are hygroscopic because of the presence of calcium chloride as an impurity. In this study, we investigated the volatilization of gaseous hypochlorous acid (HOCl (g) ) from Ca (OCl) 2 tablets and its disinfection efficacy against Staphylococcus aureus on wet agar plates were studied in a 75 m3 room. HOCl (g) volatilization occurred spontaneously and continuously, even in ambient air. Under airflow conditions, the absorption of water vapor from the air into the Ca (OCl) 2 tablet was accelerated, and numerous droplets were formed on the tablet surface. The deliquescent property of calcium chloride indices these phenomena. When a glass Petri dish containing 10 Ca (OCl) 2 tablets was placed in the room under airflow conditions, HOCl (g) volatilization of was enhanced, and the HOCl (g) concentration in the room was maintained within the range of 60‒80 ppb for at least 24 h of volatilization. Viable S. aureus count on wet agar plates placed on the floor 1‒5 m away from the tablets decreased by 3.1-log after 1.5 h of exposure. This study indicates that sufficient concentrations of HOCl (g) can be volatilized from Ca (OCl) 2 tablets without the use of water, contributing to the disinfection of attached bacteria.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 1","pages":"13-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The detection method for malodor in cotton fabrics and the impact of disinfection on malodor suppression. 棉织物中恶臭的检测方法及消毒对恶臭抑制的影响。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.3_89

Hiroaki Okuda, Mugihei Ikemizu, Yoshinobu Matsumura

引用次数: 0

Assessment of residual contamination on dental instruments after infection control practices. 感染控制后牙科器械残留污染的评估。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.3_63

Kyoungnam Kim, Soomyoung Bae, Hyojin Lee, Hyeyoung Yoon

{"title":"Assessment of residual contamination on dental instruments after infection control practices.","authors":"Kyoungnam Kim, Soomyoung Bae, Hyojin Lee, Hyeyoung Yoon","doi":"10.4265/jmc.30.3_63","DOIUrl":"https://doi.org/10.4265/jmc.30.3_63","url":null,"abstract":"This study aimed to compare and assess residual microorganisms and organic materials on selected dental instruments after practicing two infection control methods, including the standard protocol. Scaler tips (ST) , 3-way syringe tips（3ST) , Bristle brushes（BB) , and Rubber cups（RC) were collected and grouped into Standard (Group S) and Non-standard (Group N) for processing. In ST and 3ST, Group S was cleaned and autoclaved, while Group N was disinfected. For BBs and RCs, Group N was cleaned and autoclaved, and new instruments were used as Group S. To confirm the presence of residual microorganisms, instruments from each group were incubated in liquid and solid medium. The generated colonies on solid medium were identified at the species level by polymerase chain reaction (PCR) . To confirm the presence of residual organisms, each instrument was stained with Phloxine B and observed using a stereomicroscope. Only ST（8.0%) and 3ST (28.0%) samples from Group N were detected with residual microorganisms. The identified colonies included Staphylococcus, Cupriavidus, and Streptococcus spp. Residual organics were observed in all samples from Group S and N. These findings highlight the limitations of cleaning followed by autoclaving and especially disinfection in completely eradicating all microorganisms and organics.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 3","pages":"63-70"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0