Journal of microorganism control最新文献

Bacterial flora analyses and isolation of adhesive bacteria on fabrics washed by home laundry. 家用洗衣机洗涤织物上粘附细菌的菌群分析与分离。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.1_1

Hiroaki Okuda, Hirofumi Noda, Mugihei Ikemizu, Katsuya Wakita, Yoshinobu Matsumura

{"title":"Bacterial flora analyses and isolation of adhesive bacteria on fabrics washed by home laundry.","authors":"Hiroaki Okuda, Hirofumi Noda, Mugihei Ikemizu, Katsuya Wakita, Yoshinobu Matsumura","doi":"10.4265/jmc.30.1_1","DOIUrl":"10.4265/jmc.30.1_1","url":null,"abstract":"In this report, microbial cells on washed fabrics and in washing water in home laundry were analyzed, in order to suppress malodors and construct a highly sanitary home laundry system. Many bacterial cells (109-1010 cells/g dry fabric) were detected from washed fabrics emitting relative strong malodors even after washing. Detergents with or without antimicrobial agents partially removed the microorganism cells and more than 20% of cells survived even after washing and drying in almost all households. Bacterial flora analyses of washed fabrics revealed the presence of several typical genera: Enhydrobacter sp., Paracoccus sp., Sphingomonas sp., Roseomonas sp. and Kocuria sp. These results indicated that it is difficult to remove or eliminate microbial cells adhering to clothes and fabrics in home laundry. This report also indicated certain typical bacteria (adhesive type) seem to adhere or attach to fabrics, while bacteria (transient type) found in washing water have low adhesivity to fabrics. Therefore, understanding the adhesive properties of bacteria on fabrics may permit more efficient removal of microorganisms and reduction of malodors in home laundry.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of a Membrane Filter Protocol for Direct Identification of Microorganisms in Dog Ear Discharge using MALDI-TOF MS. MALDI-TOF质谱法直接鉴定犬耳分泌物微生物的膜过滤方案研究。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.2_33

Hiroto Maeda, Miki Sawatari, Momori Shimizu, Takayuki Ishige, Toshibumi Taniguchi, Toshifumi Watanabe, Kazuyuki Sogawa

{"title":"Investigation of a Membrane Filter Protocol for Direct Identification of Microorganisms in Dog Ear Discharge using MALDI-TOF MS.","authors":"Hiroto Maeda, Miki Sawatari, Momori Shimizu, Takayuki Ishige, Toshibumi Taniguchi, Toshifumi Watanabe, Kazuyuki Sogawa","doi":"10.4265/jmc.30.2_33","DOIUrl":"https://doi.org/10.4265/jmc.30.2_33","url":null,"abstract":"Otitis externa is an ear disease that is present in 10-20% of dogs. Bacterial organisms are a common cause, and infection may cause severe and chronic inflammation. Thus, there is a need for early detection and identification of bacteria in ear discharge samples. In this study, 31 such samples were analyzed to identify microorganisms using MALDI-TOF MS with pretreatment using a membrane filtration protocol and a rapid BACpro II kit, compared to pretreatment with a rapid BACpro II kit alone. Reliable scores for E. coli and E. faecalis were obtained after inoculation of samples with 1.0×104 CFU/ml using the membrane filter with the rapid BACpro II kit, with approximately 10-fold higher sensitivity compared to rapid BACpro pretreatment alone. Among 31 bacteria-positive colonies in the samples, MALDI-TOF MS and membrane filtration (0.80 µm) with a rapid BACpro II kit was significantly more accurate compared to use of the kit alone (29 (82.9%) vs. 17 (48.6%) isolates identified, p<0.001). These results show that MALDI-TOF MS with a membrane filter and a rapid BACpro II kit is a quick and reliable method for bacterial identification in ear discharge samples.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 2","pages":"33-39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A theoretical model for evaluating a specific mode of lethal injury that causes reproductive death in an irradiated microbial population. 一个理论模型，用于评估一种特定模式的致命伤害，导致生殖死亡的辐照微生物群体。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.2_55

Tetsuaki Tsuchido, Khanh C Vo, Ryoko Asada, Masakazu Furuta

引用次数: 0

Volatilization of gaseous hypochlorous acid from calcium hypochlorite product induced by the deliquescent property of calcium chloride. 氯化钙潮解特性引起的次氯酸钙产品气态次氯酸的挥发。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.1_13

Soshi Omura, Hideyuki Seki, Mami Ishikawa, Satoshi Fukuzaki

{"title":"Volatilization of gaseous hypochlorous acid from calcium hypochlorite product induced by the deliquescent property of calcium chloride.","authors":"Soshi Omura, Hideyuki Seki, Mami Ishikawa, Satoshi Fukuzaki","doi":"10.4265/jmc.30.1_13","DOIUrl":"10.4265/jmc.30.1_13","url":null,"abstract":"The commercial products of calcium hypochlorite (Ca（OCl) 2) , a solid hypochlorite salt, are hygroscopic because of the presence of calcium chloride as an impurity. In this study, we investigated the volatilization of gaseous hypochlorous acid (HOCl (g) ) from Ca (OCl) 2 tablets and its disinfection efficacy against Staphylococcus aureus on wet agar plates were studied in a 75 m3 room. HOCl (g) volatilization occurred spontaneously and continuously, even in ambient air. Under airflow conditions, the absorption of water vapor from the air into the Ca (OCl) 2 tablet was accelerated, and numerous droplets were formed on the tablet surface. The deliquescent property of calcium chloride indices these phenomena. When a glass Petri dish containing 10 Ca (OCl) 2 tablets was placed in the room under airflow conditions, HOCl (g) volatilization of was enhanced, and the HOCl (g) concentration in the room was maintained within the range of 60‒80 ppb for at least 24 h of volatilization. Viable S. aureus count on wet agar plates placed on the floor 1‒5 m away from the tablets decreased by 3.1-log after 1.5 h of exposure. This study indicates that sufficient concentrations of HOCl (g) can be volatilized from Ca (OCl) 2 tablets without the use of water, contributing to the disinfection of attached bacteria.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 1","pages":"13-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The generation of hydrogen peroxide and antibacterial effectiveness by copper oxide surface layers. 过氧化氢的产生和抗菌效果由氧化铜表面层。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.1_19

Yurika Taniguchi, Hiroshi Kawakami, Sadao Komemushi, Ken Hirota, Takashi Ozawa, Kazunori Miyamoto, Hiroaki Nakayama, Masahiko Wada

{"title":"The generation of hydrogen peroxide and antibacterial effectiveness by copper oxide surface layers.","authors":"Yurika Taniguchi, Hiroshi Kawakami, Sadao Komemushi, Ken Hirota, Takashi Ozawa, Kazunori Miyamoto, Hiroaki Nakayama, Masahiko Wada","doi":"10.4265/jmc.30.1_19","DOIUrl":"10.4265/jmc.30.1_19","url":null,"abstract":"Copper is known as an antibacterial material. Reactive oxygen species (ROS) with antibacterial effectiveness are generated on copper surfaces mainly by the Fenton-type reaction. The antibacterial effectiveness is higher in Cu2O than in CuO. In this study we discussed the effects of the difference in the amount of generated ROS on the difference in the antibacterial effectiveness between Cu2O and CuO. Both Cu2O and CuO produced hydrogen peroxide (H2O2）, hydroxyl radical(･OH）, and singlet oxygen (1O2）, but not superoxide radical (･O2-）. The concentration of H2O2 produced was higher in Cu2O than in CuO. When catalase, a scavenger of H2O2, was added, the antibacterial activities of both Cu2O and CuO reduced to almost the same value. These experimental results indicate that Cu2O is higher in antibacterial effectiveness than CuO because Cu2O produced more H2O2 than CuO. As ･OH was detected even when H2O2 was scavenged by catalase before it reacted with Cu ion, a part of ･OH was generated by chemical reactions different from the Fenton-type reaction when copper oxides were in contact with water.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 1","pages":"19-26"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of the sophoroselipid-copper complex and its bactericidal properties. 槐磷脂-铜配合物的合成及其杀菌性能。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.2_41

Yuichi Kumashita, Hiroki Masuda, Taichi Kamo, Reiko Matsumura, Miho Sasaki, Yoshihiko Hirata, Yoshinobu Matsumura

{"title":"Synthesis of the sophoroselipid-copper complex and its bactericidal properties.","authors":"Yuichi Kumashita, Hiroki Masuda, Taichi Kamo, Reiko Matsumura, Miho Sasaki, Yoshihiko Hirata, Yoshinobu Matsumura","doi":"10.4265/jmc.30.2_41","DOIUrl":"https://doi.org/10.4265/jmc.30.2_41","url":null,"abstract":"The copper (II) ion is valuable as an antimicrobial reagent and is highly safe for humans, animals, plants, and the environment. In this study, the effect of sodium sophoroselipid (SL-Na) on the bactericidal activity of CuSO4 was investigated. SL-Na enhanced the bactericidal activity of CuSO4 when incubated with it for 10 min or more at 20℃. Its bactericidal activity is significant against gram-negative bacterial cells. CuSO4 at a concertation of 100 µM, when mixed with 100 µM SL-Na, effectively killed Escherichia coli cells at a rate of more than one order of magnitude higher than that observed without SL-Na after 30 minutes at 20℃. The structural differences between SL-Na, CuSO4 and their mixture were confirmed by ultraviolet visible absorption spectrum analyses, resulting in the absorption of ca. 800 nm derived from copper (II) ions shifting to ca. 680 nm in the mixture. This shift suggests that the acid-form sophoroselipids and copper (II) ions bind weakly to form a complex (SL-Cu complex）. The purified SL-Cu complex also revealed a unique absorption at approximately 1,600 cm-1 using a Fourier transform infrared analysis and demonstrated a higher bactericidal activity than CuSO4. Our results indicate that SL-Cu complex formation enhanced the bactericidal activity of copper (II) ions.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 2","pages":"41-48"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral local factors affecting candida colonization in patients undergoing perioperative oral care. 围手术期口腔护理患者口腔局部因素对念珠菌定植的影响。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.1_27

Kei Fujishima, Tetsuya Sakuta, Naofumi Tamaki

{"title":"Oral local factors affecting candida colonization in patients undergoing perioperative oral care.","authors":"Kei Fujishima, Tetsuya Sakuta, Naofumi Tamaki","doi":"10.4265/jmc.30.1_27","DOIUrl":"10.4265/jmc.30.1_27","url":null,"abstract":"Recently, perioperative oral function management, focusing on oral care, has been considered important from the perspective of preventing infections caused by oral microorganisms. One of the infectious diseases that should be prevented is oral candidiasis. The purpose of this study was to clarify the oral local factors related to Candida colonization in perioperative patients. The analyses of the relationship between oral local factors and Candida colonization revealed that three factors - a reduction in the number of remaining teeth, wearing dentures, and increased dry mouth - were related to Candida colonization. The oral local factors related to Candida colonization identified in this study may be useful indicators for easily and quickly determining whether or not Candida colonization is present. It is difficult to perform bacteriological tests on all perioperative patients, who are subject to various constraints, including physical, mental, and time constraints, so we hope that oral examinations focusing on these oral local factors would provide a foothold in preventing the onset of oral candidiasis in perioperative patients.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"30 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143732588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of peracetic acid cleaning agents on biofilms by Methylobacterium spp. derived from hemodialysis equipment. 过氧乙酸清洗剂对血液透析设备衍生甲基杆菌生物膜的影响。

Journal of microorganism control Pub Date : 2025-01-01 DOI: 10.4265/jmc.30.2_49

Eiichi Osono, Kazumi Honda, Yuki Inoue, Kyoko Ichimura, Hideki Negishi, Takuya Kumagai, Takeo Shimizu, Naoya Miyazawa, Mizuki Tomita, Akane Kobayashi, Ryoichi Akiyama, Chisako Kamano, Shun Takaku, Toshio Akimoto, Yoshihiko Norose, Rimpei Morita

引用次数: 0

Volatilization and disinfection efficacy of gaseous hypochlorous acid from an air washer-type humidifier in a large space. 大空间中空气洗涤器型加湿器产生的气态次氯酸的挥发和消毒效果。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.3_105

Shun Nojima, Soshi Omura, Satoshi Fukuzaki

{"title":"Volatilization and disinfection efficacy of gaseous hypochlorous acid from an air washer-type humidifier in a large space.","authors":"Shun Nojima, Soshi Omura, Satoshi Fukuzaki","doi":"10.4265/jmc.29.3_105","DOIUrl":"https://doi.org/10.4265/jmc.29.3_105","url":null,"abstract":"An air washer-type humidifier has two useful functions: humidification, and air purification, and it applies to large indoor spaces. In this study, the efficacy of an air washer-type humidifier fed with 24 L of weakly acidic electrolyzed water（WAEW) at pH 5.0 and 30 mg/L in disinfecting attached bacteria and airborne microorganisms was studied in a 480 m3 indoor space. The humidifier was operated at a shower volume of 9.0 L/min of WAEW and at an air flow rate of 29 m3/min. Volatilization of gaseous hypochlorous acid（HOCl(g)) proceeded according to first-order kinetics during the 60 min of operation. Fresh WAEW was supplied to the humidifier every 60 min, and the HOCl(g) concentration in the indoor space was maintained within the range of 25-52 ppb for at least 180 min of operation. The number of viable bacterial cells on wet agar plates placed on the floor at a distance of 5-20 m away from the humidifier decreased by 2.0-3.0 log after 30 min of operation, and no viable cells were detected after 60 min of operation. A logarithmic reduction of more than 2.7 was achieved within 15 min against bacteria-attached plates placed at a 1.5 m-height position where the outlet airflow from the humidifier was directly exposed. This indicates that the disinfection efficacy of HOCl(g) volatilized from the humidifier depends on the rate of outlet airflow reaching the bacteria-attached plates. The number of viable airborne microorganisms decreased by approximately 54% after 180 min of operation. This study demonstrated that an air-washer-type humidifier can spread HOCl(g) evenly throughout a large indoor space and is effective in disinfecting attached bacteria and airborne microorganisms.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 3","pages":"105-111"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethambutol inhibited the growth of acid-fast bacteria and enhanced the detection of Legionella in environmental water samples. 乙胺丁醇可抑制酸性无菌细菌的生长，并增强对环境水样中军团菌的检测。

Journal of microorganism control Pub Date : 2024-01-01 DOI: 10.4265/jmc.29.1_1

Hiroaki Inoue, Marin Taguchi, Manami Kitazume, Yukie Saito, Tomoyuki Iwasawa

{"title":"Ethambutol inhibited the growth of acid-fast bacteria and enhanced the detection of Legionella in environmental water samples.","authors":"Hiroaki Inoue, Marin Taguchi, Manami Kitazume, Yukie Saito, Tomoyuki Iwasawa","doi":"10.4265/jmc.29.1_1","DOIUrl":"10.4265/jmc.29.1_1","url":null,"abstract":"The growth of acid-fast bacteria often hinders the detection of Legionella in water samples on agar plates by the plate culture method. We studied whether anti-tubercular agents inhibit acid-fast bacteria growth on agar plates. First, the antimicrobial activities of isoniazid, ethionamide, and ethambutol were evaluated against Mycobacterium and Legionella. We found that ethambutol at ≥ 100 μg/mL completely inhibited Mycobacterium growth, but ethambutol at 1,000 μg/mL did not inhibit Legionella growth. Next, the effect of ethambutol dissolved in acid buffer was examined. Cell suspensions of L. pneumophila and Mycobacterium spp. were mixed, and ethambutol-acid buffer was added. After 5 min, mixtures were inoculated on GVPC agar plates and incubated at 36℃ for 6 d. We found that ethambutol inhibited Mycobacterium growth on agar plates, but the Legionella colonies recovered. The effect of ethambutol was also significant in the evaluation using bathwaters. Comparing 1,302 bathwaters, the addition of ethambutol reduced the detection rate of acid-fast bacteria from 30.6% to 0% and increased the detection rate of Legionella from 7.1% to 7.5%. Ethambutol, which selectively inhibited acid-fast bacteria growth, enhanced the detection of Legionella on agar plates and will contribute to improving the accuracy of Legionella testing by the plate culture method.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"29 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140178061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0