Journal of microorganism control最新文献_第6页

Inactivation mechanism of SARS-CoV-2 by ozone in aqueous and gas phases. 臭氧在水相和气相中灭活SARS-CoV-2的机理

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.2_43

Yoshinori Nishiki, Tatsuya Imazu, Katsuhiko Nakamuro, Hirotaka Naitou, Koichi Jeremiah Aoki

{"title":"Inactivation mechanism of SARS-CoV-2 by ozone in aqueous and gas phases.","authors":"Yoshinori Nishiki, Tatsuya Imazu, Katsuhiko Nakamuro, Hirotaka Naitou, Koichi Jeremiah Aoki","doi":"10.4265/jmc.28.2_43","DOIUrl":"https://doi.org/10.4265/jmc.28.2_43","url":null,"abstract":"The comparison of the inactivation rate of SARS-CoV-2 by ozone in water with that in gas, based on data from references and experiments, has indicated the inactivation rate of the former is remarkably higher than that of the latter. To investigate the reason for this difference, we analyzed the reaction rate using a diffusional reaction model, in which ozone is carried by micro spherical viruses to inactivate the target viruses. Using this model, we can evaluate the amount of ozone required to inactivate a virus based on the ct value. We found that inactivation in gas phase requires 1014-1015 ozone molecules per virus virion, while the inactivation in aqueous phase requires 5×1010 to 5×1011 ozone molecules. This implies that the efficiency in gas phase is 200-20,000 times lower than that in aqueous phase. This is not attributed to the lower probability of collision in gas phase than in aqueous phase. Rather, it may be due to the fact that the ozone and radicals generated by ozone react and subsequently dissipate. We proposed the diffusion of ozone into a spherical virus at a steady state and the decomposition reaction model through radicals.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 2","pages":"43-48"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theory and application of growth delay analysis of colony formation for evaluation of injured population of the stressed fungal conidia. 菌落形成生长延迟分析在真菌分生孢子损伤种群评估中的理论和应用。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_93

Ryoko Asada, Yoshie Yamada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido

{"title":"Theory and application of growth delay analysis of colony formation for evaluation of injured population of the stressed fungal conidia.","authors":"Ryoko Asada, Yoshie Yamada, Jin J Sakamoto, Masakazu Furuta, Tetsuaki Tsuchido","doi":"10.4265/jmc.28.3_93","DOIUrl":"10.4265/jmc.28.3_93","url":null,"abstract":"A new concept of injured population assessment is proposed, in which the size of the injured population in stressed mold spores is evaluated by analyzing the colony formation process on a solid agar medium. In this method, a small paper disc containing mold spores is placed on a subculture agar plate, and the linear increase in the radius of the colony formed by development from the spore is measured over time. Then, the principle of the previously reported growth delay analysis (GDA) method originally using a liquid medium is applied to obtain the integrated viable ratio (IV) of the stressed population from the delay time relative to the growth of the unstressed population. On the other hand, the viable ratio (V) to the initial value as the colony count obtained with the stressed culture is obtained; the difference between the logarithms of V and IV is determined as the log number of the injured population. Applying this analysis method to heated spores of Cladosporium sphaerospermum, we determined the size of the injured population that occurred. This method was considered to be effective as a new method for quantifying injured populations using a solid medium.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"93-100"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Common presence of plasmid encoding bla_CTX-M-55 in extended-spectrum β-lactamase-producing Salmonella enterica and Escherichia coli isolates from the same edible river fish. 编码blaCTX-M-55的质粒在产β-内酰胺酶的肠炎沙门氏菌和大肠杆菌分离株中普遍存在。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.2_49

Tatsuya Nakayama, Shiori Yamamoto, Natsuki Ohata, Takahiro Yamaguchi, Michio Jinnai, Doan Tran Nguyen Minh, Oanh Nguyen Hoang, Hien LE Thi, Phong Ngo Thanh, Phuong Hoang Hoai, Phuc Nguyen DO, Chinh Dang VAN, Yuko Kumeda, Atsushi Hase

{"title":"Common presence of plasmid encoding blaCTX-M-55 in extended-spectrum β-lactamase-producing Salmonella enterica and Escherichia coli isolates from the same edible river fish.","authors":"Tatsuya Nakayama, Shiori Yamamoto, Natsuki Ohata, Takahiro Yamaguchi, Michio Jinnai, Doan Tran Nguyen Minh, Oanh Nguyen Hoang, Hien LE Thi, Phong Ngo Thanh, Phuong Hoang Hoai, Phuc Nguyen DO, Chinh Dang VAN, Yuko Kumeda, Atsushi Hase","doi":"10.4265/jmc.28.2_49","DOIUrl":"10.4265/jmc.28.2_49","url":null,"abstract":"The transmission of potentially life-threatening plasmid-mediated antibiotic-resistant bacteria poses a major threat to public health. This study aimed to determine the presence of commonly observed plasmids encoding plasmid-mediated antibiotic-resistance genes in Salmonella and Escherichia coli isolates from fishery products. Eighty river fishes were purchased from retail stores and supermarkets in Vietnam. Only Salmonella-positive fishes were used for antibiotic-resistant E. coli isolation. Salmonella serotyping was performed using Salmonella antisera. Isolated bacterial DNA was extracted, and antibiotic susceptibility, resistance genes, and replicon typing were determined. Our results showed that Salmonella was isolated from 12.5% (10/80) of the river fishes. Cefotaxime-resistant Salmonella was isolated from 3.8% (3/80) of the fishes and colistin-resistant Salmonella from 1.3% (1/80) . Salmonella serotyping revealed Potsdam, Schwarzengrund, Bardo/Newport, Give, Infantis, Kentucky, and Typhimurium. Multiplex polymerase chain reaction revealed the presence of extended-spectrum β-lactamase-related genes blaCTX-M-55 and blaCTX-M-65 and the colistin resistance gene mcr-1. To date, no study has reported an antibiotic-resistance plasmid present in multiple bacteria collected from the same food. Thus, horizontal transmission of antibiotic-resistance plasmids may occur at the food level.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 2","pages":"49-56"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10120753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Five-year trends in bacterial contamination of dialysis equipment. 透析设备细菌污染的五年趋势。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_69

Eiichi Osono, Kazumi Honda, Yuki Inoue, Kyouko Ichimura, Chisako Kamano, Shinya Kawamoto, Yoshihiko Norose, Shun Takaku, Rimpei Morita, Tetsuaki Tsuchido

{"title":"Five-year trends in bacterial contamination of dialysis equipment.","authors":"Eiichi Osono, Kazumi Honda, Yuki Inoue, Kyouko Ichimura, Chisako Kamano, Shinya Kawamoto, Yoshihiko Norose, Shun Takaku, Rimpei Morita, Tetsuaki Tsuchido","doi":"10.4265/jmc.28.3_69","DOIUrl":"10.4265/jmc.28.3_69","url":null,"abstract":"Bedside dialysis monitoring equipment for hemodialysis are located in the bioburden section upstream of the endotoxin-retentive filter for dialysis fluid sterilization. We observed 26 equipment at our institution for bacterial contamination at least once every 4 weeks for 5 years with another ultrafiltration membrane upstream to prevent bacterial contamination. Bacterial contamination levels were highest and most diverse at the time of the first flush. During subsequent initial cleanng, the contamination level decreased, and bacterial species converged almost exclusively to one genus, namely Methylobacterium spp. During clinical use, the equipment were cleaned and disinfected daily after dialysis, and daily operations and maintenance were performed using aseptic techniques. Although the frequency of bacterial detection decreased annually, the same bacterial genotypes observed at the first flush were isolated even after long time periods and were thought to persist in the equipment possibly by forming biofilm. Pseudomonas aeruginosa was newly detected after the replacement of parts during breakdown maintenance, indicating the need to sterilize replacement parts. Thus, the bioburden should be assessed regularly as part of the management of in-house-produced dialysis fluid.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"69-75"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New synergistic antibacterial mechanism of bulky mixed Ti/w hetero-polyoxometalates composed of multi lacunary Keggin structure with oxacillin against vancomycin intermediate-resistant Staphylococcus aureus. 由多空隙Keggin结构和苯唑西林组成的大体积混合Ti/w杂多金属氧酸盐对万古霉素中间体耐药金黄色葡萄球菌的协同抗菌新机制。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_101

Jun Iijima, Toshihiro Yamase

{"title":"New synergistic antibacterial mechanism of bulky mixed Ti/w hetero-polyoxometalates composed of multi lacunary Keggin structure with oxacillin against vancomycin intermediate-resistant Staphylococcus aureus.","authors":"Jun Iijima, Toshihiro Yamase","doi":"10.4265/jmc.28.3_101","DOIUrl":"10.4265/jmc.28.3_101","url":null,"abstract":"Considering the lack of detailed research on the antibacterial mechanism of polyoxometalates, we examined the synergistic effect of novel bulky mixed Ti/W hetero-polyoxometalates (K9.5H2.5 ［α-Ge2Ti4W20O78］・ 29H2O; αTi4, K9H5 ［α-Ge2Ti6W18O77］・16H2O; αTi6, K23H5［α-Ge4Ti12W36O154］・39H2O; αTi12, K9H5 ［β-Ge2Ti6W18O77］・ 45H2O; βTi6) with the antibiotic oxacillin against vancomycin intermediate-resistant Staphylococcus aureus (VISA) using fractional inhibitory concentration (FIC) index and growth curve in this study. All polyoxometalates used in this study showed remarkable synergistic effects with oxacillin. Its synergistic antibacterial mechanism was examined using reverse transcription PCR (RT-PCR) and penicillin binding protein-2' (PBP2') latex agglutination test. The results suggested that these polyoxometalates did not inhibit mecA gene transcription but resulted in PBP2' protein malfunction. From these results, we concluded that the substances producing resistance in VISA were affected by polyoxometalates depending on their molecular size, facilitating a synergistic antibacterial effect with oxacillin.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"101-107"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viability of sublethally injured bacteria of fresh and fresh-cut vegetables from the field through distribution. 新鲜蔬菜和鲜切蔬菜从田间到配送过程中的亚出血损伤细菌存活率。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.4_153

Hidemi Izumi

{"title":"Viability of sublethally injured bacteria of fresh and fresh-cut vegetables from the field through distribution.","authors":"Hidemi Izumi","doi":"10.4265/jmc.28.4_153","DOIUrl":"10.4265/jmc.28.4_153","url":null,"abstract":"Bacterial stresses can occur from the production to the distribution environments of produce, and these stresses can lead to nonlethal bacterial damage that is an injured state called sublethally injured bacteria. The damage is mainly due to the disruption of the surface structure and cytoplasmic membrane of the cells. Sublethally sanitizer-injured indicator coliform bacteria injured by chlorine, ethanol, and/or fungicide stress could exhibit on vegetables during production and harvest. Chlorine stress and cold stress could induce sublethally injured indicator and pathogenic coliform bacteria on fresh-cut vegetables during processing and subsequent storage. Enterobacter kobei and Pantoea ananatis injurd by chlorine stress, E. amnigenus, E. asburiae, and E. kobei injured by ethanol stress, and Rahnella aquatilis, Yersinia mollaretii, and Escherichia coli injured by fungicide stress could be amongst the injured cells in the coliforms detected in the produce environments. To ensure the microbiological quality and safety of fresh-cut vegetables, it is necessary to adjust the concentration of sanitizer to a level that kills bacteria and does not produce sanitizer- injured cells when sanitizer is applied to the produce, and also to consider the storage temperature to inhibit the recovery of injured bacteria due to cold injury during the chilling storage period.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"153-163"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current circumstances and prospects on performance evaluation for infection control technologies of airborne viruses in indoorenvironments. 室内环境中空气传播病毒感染控制技术性能评估的现状与前景。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.4_177

Noriko Shimasaki

{"title":"Current circumstances and prospects on performance evaluation for infection control technologies of airborne viruses in indoorenvironments.","authors":"Noriko Shimasaki","doi":"10.4265/jmc.28.4_177","DOIUrl":"10.4265/jmc.28.4_177","url":null,"abstract":"Respiratory infectious diseases have potential of aerosol transmission such as COVID-19. The development of new technologies for infection control against airborne viruses are further required. It is necessary for effective development to evaluate properly the effect and role of these technologies in indoor environment. Here, the author provided essential knowledge for infection control of viral aerosols, i.e., basic concept of infection control, features of COVID-19 and Influenza including the entry receptor in body of each virus, behavior of the viral aerosols released from patient bodies, and Wells-Riley model as a traditional quantitative assessment of the infection risk by aerosol transmission. Previous evaluation studies on airborne viruses were categorized into three types of experiments, namely, in vitro, in vivo, and in humans and real indoor environments. Some prospects were described, including standard evaluation methods for air cleaners, the research group to formulate guidelines for evaluating the hygienic effects of chemical substances on microbes in real indoor space, and personal opinions on evaluation concept linked to three types of experiments. This minireview may help to correctly evaluate the hygienic effects of control technologies against airborne viruses in indoor environment and to contribute development of technologies with required performance according to infection risk.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"177-186"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excessive ultraviolet C irradiation causes spore protein denaturation and prohibits the initiation of spore germination in Bacillus subtilis. 过度的紫外线C照射会导致芽孢杆菌的孢子蛋白变性，并阻止芽孢萌发的开始。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.1_15

Ritsuko Kuwana, Ryuji Yamazawa, Ryoko Asada, Kiyoshi Ito, Masakazu Furuta, Hiromu Takamatsu

{"title":"Excessive ultraviolet C irradiation causes spore protein denaturation and prohibits the initiation of spore germination in Bacillus subtilis.","authors":"Ritsuko Kuwana, Ryuji Yamazawa, Ryoko Asada, Kiyoshi Ito, Masakazu Furuta, Hiromu Takamatsu","doi":"10.4265/jmc.28.1_15","DOIUrl":"https://doi.org/10.4265/jmc.28.1_15","url":null,"abstract":"Ultraviolet (UV) -C is widely used to kill bacteria as it damages chromosomal DNA. We analyzed the denaturation of the protein function of Bacillus subtilis spores after UV-C irradiation. Almost all of the B. subtilis spores germinated in Luria-Bertani (LB) liquid medium, but the colony-forming unit (CFU) of the spores on LB agar plates decreased to approximately 1/103 by 100 mJ/cm2 of UV-C irradiation. Some of the spores germinated in LB liquid medium under phase-contrast microscopy, but almost no colonies formed on the LB agar plates after 1 J/cm2 of UV-C irradiation. The fluorescence of the green fluorescent protein (GFP) -fused spore proteins, YeeK-GFP, YeeK is a coat protein, decreased following UV-C irradiation of over 1 J/cm2, while that of SspA-GFP, SspA is a core protein, decreased following UV-C irradiation of over 2 J/ cm2, respectively. These results revealed that UV-C affected on coat proteins more than core proteins. We conclude that 25 to 100 mJ/cm2 of UV-C irradiation can cause DNA damage, and more than 1 J/cm2 of UV-C irradiation can cause the denaturation of spore proteins involved in germination. Our study would contribute to improve the technology to detect the bacterial spores, especially after UV sterilization.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 1","pages":"15-25"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9582668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivation efficacy of low-pressure plasma treatment against seed-borne tomato pathogen Clavibacter michiganensis and effect of seed setting position and mesh sheet usage. 低压等离子体处理对种子传播的番茄病原体密歇根Clavibacter Miciganensis的灭活效果以及结实位置和网片使用的影响。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_123

Terumi Nishioka, Yuichiro Takai, Tomoko Mishima, Hideo Tanimoto, Kiyotsugu Okada, Tatsuya Misawa, Shinichi Kusakari

{"title":"Inactivation efficacy of low-pressure plasma treatment against seed-borne tomato pathogen Clavibacter michiganensis and effect of seed setting position and mesh sheet usage.","authors":"Terumi Nishioka, Yuichiro Takai, Tomoko Mishima, Hideo Tanimoto, Kiyotsugu Okada, Tatsuya Misawa, Shinichi Kusakari","doi":"10.4265/jmc.28.3_123","DOIUrl":"10.4265/jmc.28.3_123","url":null,"abstract":"Clavibacter michiganensis, a gram-positive actinomycete, is a major seed-borne tomato pathogen. We investigated the inactivation efficacy of low-pressure plasma treatment against C. michiganensis inoculated on tomato seeds by placing them on a mesh sheet above the bottom dielectric glass plate. The 2- and 5-minute plasma treatment reduced C. michiganensis populations on the tomato seeds by 0.8 and 1.8 log cfu/seed, respectively. The reduction rates were similar to those of C. michiganensis on shirona (cruciferous) seeds, which have different shapes and surface structures. In contrast, the inactivation of C. michiganensis cells using plasma was more difficult than that of X. campestris cells. Additionally, it was found that placing seeds on a mesh sheet laid on the dielectric glass plate was remarkably effective in inactivating the pathogens on tomato seeds. Since the tomato seeds were susceptible to damage from plasma treatment, methods to reduce its damage need to be investigated.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"123-128"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of natural extracts with anti-norovirus effects and analysis of this mechanism in grape seed extract. 具有抗诺如病毒作用的天然提取物的筛选和葡萄籽提取物中这一机制的分析。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_83

Kota Nishitani, Takayuki Morita, Yoshiyuki Seto

{"title":"Screening of natural extracts with anti-norovirus effects and analysis of this mechanism in grape seed extract.","authors":"Kota Nishitani, Takayuki Morita, Yoshiyuki Seto","doi":"10.4265/jmc.28.3_83","DOIUrl":"10.4265/jmc.28.3_83","url":null,"abstract":"Norovirus (NoV）is a major causative virus of viral gastroenteritis and requires a general disinfection method because it is resistant to common disinfectants such as ethanol and chlorhexidine. This study aimed to find natural extracts as candidates for versatile disinfectant ingredients. The antiviral effect of natural extracts against NoV can be evaluated using the feline calicivirus (FCV）-inactivation test and NoV virus-like particle (NoV-VLP）-binding inhibition test. In this study, screening of natural extracts with anti- NoV effects was performed using these two methods. Of the 63 natural extracts examined, 14 were found to have high FCV-inactivation and NoV-VLP-binding inhibitory effects. In addition, we evaluated the NoV-VLPbinding inhibitory effect of grape seed extract（GSE）containing proanthocyanidins under multiple concentration conditions and treatment times and determined that the binding inhibitory effect of GSE was concentration- and time-dependent. Electron microscopy showed that GSE-treated NoV-VLPs aggregated, distorted, and swelled, suggesting that GSE directly interacts with NoV particles. The results suggest that some natural extracts containing GSE can be used as components of disinfectants against NoV.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0