Journal of microorganism control最新文献_第6页

Viability of sublethally injured bacteria of fresh and fresh-cut vegetables from the field through distribution. 新鲜蔬菜和鲜切蔬菜从田间到配送过程中的亚出血损伤细菌存活率。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.4_153

Hidemi Izumi

{"title":"Viability of sublethally injured bacteria of fresh and fresh-cut vegetables from the field through distribution.","authors":"Hidemi Izumi","doi":"10.4265/jmc.28.4_153","DOIUrl":"10.4265/jmc.28.4_153","url":null,"abstract":"Bacterial stresses can occur from the production to the distribution environments of produce, and these stresses can lead to nonlethal bacterial damage that is an injured state called sublethally injured bacteria. The damage is mainly due to the disruption of the surface structure and cytoplasmic membrane of the cells. Sublethally sanitizer-injured indicator coliform bacteria injured by chlorine, ethanol, and/or fungicide stress could exhibit on vegetables during production and harvest. Chlorine stress and cold stress could induce sublethally injured indicator and pathogenic coliform bacteria on fresh-cut vegetables during processing and subsequent storage. Enterobacter kobei and Pantoea ananatis injurd by chlorine stress, E. amnigenus, E. asburiae, and E. kobei injured by ethanol stress, and Rahnella aquatilis, Yersinia mollaretii, and Escherichia coli injured by fungicide stress could be amongst the injured cells in the coliforms detected in the produce environments. To ensure the microbiological quality and safety of fresh-cut vegetables, it is necessary to adjust the concentration of sanitizer to a level that kills bacteria and does not produce sanitizer- injured cells when sanitizer is applied to the produce, and also to consider the storage temperature to inhibit the recovery of injured bacteria due to cold injury during the chilling storage period.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"153-163"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excessive ultraviolet C irradiation causes spore protein denaturation and prohibits the initiation of spore germination in Bacillus subtilis. 过度的紫外线C照射会导致芽孢杆菌的孢子蛋白变性，并阻止芽孢萌发的开始。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.1_15

Ritsuko Kuwana, Ryuji Yamazawa, Ryoko Asada, Kiyoshi Ito, Masakazu Furuta, Hiromu Takamatsu

{"title":"Excessive ultraviolet C irradiation causes spore protein denaturation and prohibits the initiation of spore germination in Bacillus subtilis.","authors":"Ritsuko Kuwana, Ryuji Yamazawa, Ryoko Asada, Kiyoshi Ito, Masakazu Furuta, Hiromu Takamatsu","doi":"10.4265/jmc.28.1_15","DOIUrl":"https://doi.org/10.4265/jmc.28.1_15","url":null,"abstract":"Ultraviolet (UV) -C is widely used to kill bacteria as it damages chromosomal DNA. We analyzed the denaturation of the protein function of Bacillus subtilis spores after UV-C irradiation. Almost all of the B. subtilis spores germinated in Luria-Bertani (LB) liquid medium, but the colony-forming unit (CFU) of the spores on LB agar plates decreased to approximately 1/103 by 100 mJ/cm2 of UV-C irradiation. Some of the spores germinated in LB liquid medium under phase-contrast microscopy, but almost no colonies formed on the LB agar plates after 1 J/cm2 of UV-C irradiation. The fluorescence of the green fluorescent protein (GFP) -fused spore proteins, YeeK-GFP, YeeK is a coat protein, decreased following UV-C irradiation of over 1 J/cm2, while that of SspA-GFP, SspA is a core protein, decreased following UV-C irradiation of over 2 J/ cm2, respectively. These results revealed that UV-C affected on coat proteins more than core proteins. We conclude that 25 to 100 mJ/cm2 of UV-C irradiation can cause DNA damage, and more than 1 J/cm2 of UV-C irradiation can cause the denaturation of spore proteins involved in germination. Our study would contribute to improve the technology to detect the bacterial spores, especially after UV sterilization.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 1","pages":"15-25"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9582668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivation efficacy of low-pressure plasma treatment against seed-borne tomato pathogen Clavibacter michiganensis and effect of seed setting position and mesh sheet usage. 低压等离子体处理对种子传播的番茄病原体密歇根Clavibacter Miciganensis的灭活效果以及结实位置和网片使用的影响。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_123

Terumi Nishioka, Yuichiro Takai, Tomoko Mishima, Hideo Tanimoto, Kiyotsugu Okada, Tatsuya Misawa, Shinichi Kusakari

{"title":"Inactivation efficacy of low-pressure plasma treatment against seed-borne tomato pathogen Clavibacter michiganensis and effect of seed setting position and mesh sheet usage.","authors":"Terumi Nishioka, Yuichiro Takai, Tomoko Mishima, Hideo Tanimoto, Kiyotsugu Okada, Tatsuya Misawa, Shinichi Kusakari","doi":"10.4265/jmc.28.3_123","DOIUrl":"10.4265/jmc.28.3_123","url":null,"abstract":"Clavibacter michiganensis, a gram-positive actinomycete, is a major seed-borne tomato pathogen. We investigated the inactivation efficacy of low-pressure plasma treatment against C. michiganensis inoculated on tomato seeds by placing them on a mesh sheet above the bottom dielectric glass plate. The 2- and 5-minute plasma treatment reduced C. michiganensis populations on the tomato seeds by 0.8 and 1.8 log cfu/seed, respectively. The reduction rates were similar to those of C. michiganensis on shirona (cruciferous) seeds, which have different shapes and surface structures. In contrast, the inactivation of C. michiganensis cells using plasma was more difficult than that of X. campestris cells. Additionally, it was found that placing seeds on a mesh sheet laid on the dielectric glass plate was remarkably effective in inactivating the pathogens on tomato seeds. Since the tomato seeds were susceptible to damage from plasma treatment, methods to reduce its damage need to be investigated.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"123-128"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of natural extracts with anti-norovirus effects and analysis of this mechanism in grape seed extract. 具有抗诺如病毒作用的天然提取物的筛选和葡萄籽提取物中这一机制的分析。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.3_83

Kota Nishitani, Takayuki Morita, Yoshiyuki Seto

{"title":"Screening of natural extracts with anti-norovirus effects and analysis of this mechanism in grape seed extract.","authors":"Kota Nishitani, Takayuki Morita, Yoshiyuki Seto","doi":"10.4265/jmc.28.3_83","DOIUrl":"10.4265/jmc.28.3_83","url":null,"abstract":"Norovirus (NoV）is a major causative virus of viral gastroenteritis and requires a general disinfection method because it is resistant to common disinfectants such as ethanol and chlorhexidine. This study aimed to find natural extracts as candidates for versatile disinfectant ingredients. The antiviral effect of natural extracts against NoV can be evaluated using the feline calicivirus (FCV）-inactivation test and NoV virus-like particle (NoV-VLP）-binding inhibition test. In this study, screening of natural extracts with anti- NoV effects was performed using these two methods. Of the 63 natural extracts examined, 14 were found to have high FCV-inactivation and NoV-VLP-binding inhibitory effects. In addition, we evaluated the NoV-VLPbinding inhibitory effect of grape seed extract（GSE）containing proanthocyanidins under multiple concentration conditions and treatment times and determined that the binding inhibitory effect of GSE was concentration- and time-dependent. Electron microscopy showed that GSE-treated NoV-VLPs aggregated, distorted, and swelled, suggesting that GSE directly interacts with NoV particles. The results suggest that some natural extracts containing GSE can be used as components of disinfectants against NoV.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injury modes and physiological characteristics of injured microorganisms with a special reference to heat injury. 受伤微生物的损伤模式和生理特征，特别是热损伤。

Journal of microorganism control Pub Date : 2023-01-01 DOI: 10.4265/jmc.28.4_187

Tetsuaki Tsuchido

{"title":"Injury modes and physiological characteristics of injured microorganisms with a special reference to heat injury.","authors":"Tetsuaki Tsuchido","doi":"10.4265/jmc.28.4_187","DOIUrl":"10.4265/jmc.28.4_187","url":null,"abstract":"Various sterilization and disinfection processes are used to control harmful microorganisms in food, medicine, and the environment. During killing, microorganisms often remain between life and death, being called injured microorganisms. The degree of injury of the injured microorganisms depends on the load of the disinfection treatment, and the treatment conditions and varies not only quantitatively but also qualitatively. Knowing how they are injured by sublethal and lethal stresses of disinfection, how they repair themselves, what makes the difference between life and death, and their physiological characteristics, will lead to appropriate microbial testing and optimization of disinfection conditions for practical viability and growth potential, and will deepen our understanding of the effectiveness of the treatment. Focusing on mainly heat injury and using Escherichia coli as a model microorganism, in this review, I will discuss the classification of injury modes in injured microorganisms caused by disinfection treatment, including \"λ injury\" (delayed resumption of growth）,\" μ injury\" (reduced growth rate injury）,\" β injury\" (secondary injury）, and other derivatives.","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"187-200"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0