{"title":"Infection control and COVID-19 outbreak training at elderly care facilities.","authors":"Naomi Sakurai, Junko Kurita, Shinobu Yamaguchi, Nami Kamioka, Tamie Sugawara, Yasushi Ohkusa","doi":"10.4265/jmc.28.3_77","DOIUrl":"10.4265/jmc.28.3_77","url":null,"abstract":"<p><p>Fatalities caused by pneumonia and underlying diseases from COVID-19 infection show the highest relative frequency among elderly people. Long-term care facilities for elderly people have continued to be the areas most vulnerable to COVID-19. We examined the effectiveness of training for infection control and COVID-19 at elderly care facilities. After sending questionnaires to all long-term elderly care facilities in Ibaraki prefecture, Japan during January 18-29, 2022, we received useful responses from 98 facilities. Using logistic regression, we regressed a dummy variable for outbreak experience to dummy variables representing routine but partial training, routine training for all staff members, long-term care facilities for elderly people, numbers of nurses, and numbers of residents. Outbreak experiences of two types were inferred, as represented by a dummy variable for a COVID-19 outbreak at the facility, and by a dummy variable for outbreak experience at the facility before COVID-19 was found. Multivariate analysis indicated routine training for all staff members as the most effective, in fact the only effective, countermeasure against COVID-19 outbreak.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"77-82"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Combined effects of microorganism control and the concept of their evaluation methods.","authors":"Tetsuaki Tsuchido","doi":"10.4265/jmc.28.4_201","DOIUrl":"10.4265/jmc.28.4_201","url":null,"abstract":"<p><p>Various combination treatments are used for microorganism control in food, medicine, and the environment. Especially in food, combination treatments have been studied using antimicrobial compounds in pasteurization and sterilization but comprehensive quantitative evaluation methods, have not yet been established to evaluate their effectiveness. This review introduces the author's recently published methods for evaluating the effects of combination treatments on the control of harmful microorganisms in food. Particularly important items are 1) the type of action of the control treatment and the mode of the combined method, 2) the choice of endpoint method and growth delay method for analytical evaluation, 3) the construction of extended isobolography that allows the application of conventional isobologram (IBo) for chemicals to various control methods, 4) the extended use of combined index (CI), and 5) the introduction of synergistic parameter (SP) for quantitative evaluation of synergistic effects. In addition, I describe the characteristics of the action of antimicrobial compounds and disinfectants in their combined effects with heating, and insist on the advantages of using combined treatments and their evaluation methods in the food industry.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"201-212"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Different patterns of germination inhibition by carvacrol and thymol in Bacillus subtilis spores.","authors":"Toshio Sakai, Jin J Sakamoto, Ryoko Asada, Masakazu Furuta, Tetsuaki Tsuchido","doi":"10.4265/jmc.28.1_3","DOIUrl":"https://doi.org/10.4265/jmc.28.1_3","url":null,"abstract":"<p><p>This study aimed to clarify how the phenolic monoterpene carvacrol and its structural isomer thymol both as essential oil components (EOCs) inhibit the germination of Bacillus subtilis spore. Germination was evaluated by the OD<sub>600</sub> reduction rate in a growth medium and phosphate buffer containing either l-alanine (l-Ala) system or l-asparagine, d-glucose, d-fructose plus KCl (AGFK) system. The germination of the wild-type spores in the Trypticase Soy broth (TSB) was found to be greatly inhibited by thymol than by carvacrol. Such a difference in the germination inhibition was confirmed by the dipicolinic acid (DPA) release from germinating spores in the AGFK buffer system, but not in the l-Ala system. Similar to the wild-type spores, no difference in the inhibitory activity between the EOCs was also indicated with the gerB, gerK-deletion mutant spores in the l-Ala buffer system and the above substantial difference was also done with the gerA-deleted mutant spores in the AGFK. Fructose was found to release spores from the EOC inhibition and inversely even stimulated. Increased concentrations of glucose and fructose partially suppressed the germination inhibition by carvacrol. The results obtained should contribute to the elucidation of the control effects of these EOCs on bacterial spores in foods.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 1","pages":"3-13"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Self-decomposition and half-life time of gaseous hypochlorous acid.","authors":"Tetsuyoshi Yamada, Takashi Muramatsu, Tatsuya Kiriyama, Satoshi Fukuzaki","doi":"10.4265/jmc.28.2_65","DOIUrl":"https://doi.org/10.4265/jmc.28.2_65","url":null,"abstract":"<p><p>The concentration of the indoor gaseous hypochlorous acid (HOCl <sub>(g)</sub>) varied significantly during disinfection. The kinetics of self-decomposition of HOCl <sub>(g)</sub> was studied at temperatures within the range of 10℃ to 40℃ and relative humidity between 30% RH and 90% RH in a lab-scale confined polyvinylidene fluoride gas bag space. The decay curve of HOCl <sub>(g)</sub> , obtained by plotting the logarithm of the HOCl <sub>(g)</sub> concentration against time, was analyzed using an integrated model that showed two simultaneous first-order processes. One process was assumed to be the adsorption of HOCl <sub>(g)</sub> onto the gas bag surface, whereas the other was the self-decomposition of HOCl <sub><sup>(g)</sup></sub> in the gas space. The decay curve is reduced to the sum of two independent and simultaneous first-order processes. The decay rate constant for self-decomposition depended on temperature and relative humidity. The half-life time of HOCl <sub>(g)</sub> was estimated to be between 76.9 h and 116 h depending on the temperature and relative humidity conditions.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 2","pages":"65-68"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10120750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the effect of peptidoglycan-constituting components on photocatalytic bactericidal sterilization.","authors":"Haruka Motomura, Tomonori Suzuki","doi":"10.4265/jmc.28.3_117","DOIUrl":"10.4265/jmc.28.3_117","url":null,"abstract":"<p><p>Previous studies in our lab have shown that peptidoglycan (PG) enhances the photocatalytic bactericidal effect. Therefore, in this study, we focused on the PG-constituting components. The PG-constituting components were added to Mesoplasma florum with no cell wall, respectively, and their effects on photocatalytic sterilization were investigated. The PG-constituting components used were amino sugars, amino acids, L-Alanine-D-Glutamic Acid (L-Ala-D-Glu) dipeptide of diaminopimelic acid (DAP)-type crosslinking peptide, and Lysine (Lys)-type crosslinking peptide. We compared the survival rates of M. florum cells and PG-constituting components-added M. florum cells after 3 h of photocatalytic reaction. Consequently, the survival rates of the cells that were added N-acetylglucosamine (GlcNAc), DAP, and L-Ala- D-Glu dipeptide were significantly lower than those of only the cells. Furthermore, the amounts of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) generated by the photocatalytic reaction under the presence of these components were determined. The results showed that DAP, L-Ala- D-Glu dipeptide, and PG of DAP-type significantly increased the amount of H<sub>2</sub>O<sub>2</sub> produced. From the above results, it is suggested that the presence of DAP and L-Ala- D-Glu dipeptide in the photocatalytic reaction boosts the production of H<sub>2</sub>O<sub>2</sub> and enhances the bactericidal effect and that GlcNAc might produce reactive oxygen species other than H<sub>2</sub>O<sub>2</sub>.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"117-122"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Possible solution of capturing viable Enterohemorrhagic Escherichia coli (EHEC) in clinical patient stool.","authors":"Hajime Teramura, Sachie Sanga, Kanako Kodama","doi":"10.4265/jmc.28.3_129","DOIUrl":"10.4265/jmc.28.3_129","url":null,"abstract":"<p><p>For clinical diagnosis of enterohemorrhagic Escherichia coli (EHEC), it needs to capture viable EHEC cells from stool sample in the view of medical fee points. However, there is no comprehensive solution for the detection of viable EHEC cells since there are wide variety of serotype and susceptibility against potassium tellurite which is commonly used for selective agent in selective medium for EHEC. In these background, EHEC Clear-HT System (EHEC-CHT), a novel effective chromogenic medium system for screening comprehensive viable EHEC, was developed. When EHEC-CHT was assessed using 128 microbes including 49 clinical isolated EHEC strains, EHEC-CHT detected all 49 EHEC strains as typical blue-colored colony regardless of both serotype and susceptibility to potassium tellurite. EHEC-CHT was compared with Japanese commercially available tellurite-based EHEC selective media using 107 clinical patient stool samples. EHEC-CHT showed higher detection ratio than conventional tellurite-based selective media compared, and 7% improvement at least in detection ratio in this study.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 3","pages":"129-134"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sustainable development for new era of microorganism control.","authors":"Satoshi Fukuzaki, Tetsuaki Tsuchido","doi":"10.4265/jmc.28.1_1","DOIUrl":"10.4265/jmc.28.1_1","url":null,"abstract":"","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Simple and quick detection of extended-spectrum β-lactamase and carbapenemase-encoding genes using isothermal nucleic acid amplification techniques.","authors":"Tatsuya Nakayama, Keisuke Soga","doi":"10.4265/jmc.28.4_145","DOIUrl":"10.4265/jmc.28.4_145","url":null,"abstract":"<p><p>The spread of plasmid-mediated antibiotic-resistant bacteria must be controlled; to this end, developing kits for simple and rapid detection in food and clinical settings is desirable. This review describes the detection of antibiotic resistance genes in extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing bacteria. Loop-mediated isothermal amplification (LAMP), a technique developed in Japan, is a useful diffusion amplification method that does not require equipment like thermal cyclers, and amplifies the target gene in 30 min at about 65℃. Although most reports targeting ESBL and carbapenemase genes are intended for clinical use, environmental and food samples have also been targeted. Recombinase polymerase amplification (RPA) has recently been developed; in RPA, the reaction proceeds under the human skin with reaction conditions of 30 min at 37℃. Detection of ESBL and carbapenemase-encoding genes in food and clinical samples using RPA has been reported in limited studies. However, research on RPA has just begun, and further development is expected.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"145-152"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoshihiko Koizumi, Tomoaki Ichijo, Kimiko Uchii, Masao Nasu
{"title":"Changes in bacterial diversity and community structure in drinking water distribution system revealed by high throughput sequencing.","authors":"Yoshihiko Koizumi, Tomoaki Ichijo, Kimiko Uchii, Masao Nasu","doi":"10.4265/jmc.28.1_27","DOIUrl":"https://doi.org/10.4265/jmc.28.1_27","url":null,"abstract":"<p><p>For microbiological management of water quality, it is important to identify bacteria and to understand the community structure. To analyze the community structure during water purification and distribution, we selected a distribution system in which water from other water treatment facilities was not mixed with the target water. Changes in the bacterial community structure during treatment and distribution processes in a slow filtration water treatment facility were analyzed using 16S rRNA gene amplicon sequencing with a portable sequencer MinION. The microbial diversity was reduced by chlorination. The genus level diversity increased during distribution and this diversity was maintained through to the terminal tap water. Yersinia and Aeromonas were dominant in the intake water, and Legionella was dominant in the slow sand filtered water. Chlorination greatly reduced the relative abundance of Yersinia, Aeromonas, and Legionella, and these bacteria were not detected in the terminal tap water. Sphingomonas, Starkeya and Methylobacterium became dominant in the water after chlorination. These bacteria could be used as important indicator bacteria to provide useful information for microbiological control in drinking water distribution systems.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 1","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9582669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uses of gaseous hypochlorous acid for controlling microorganisms in indoor spaces.","authors":"Satoshi Fukuzaki","doi":"10.4265/jmc.28.4_165","DOIUrl":"10.4265/jmc.28.4_165","url":null,"abstract":"<p><p>Hypochlorous acid (HOCl) is an active species in the chlorination process. Hypochlorite salts that release hypochlorite ion (OCl<sup>-</sup>) have been used for more than 200 years as disinfecting, cleaning, deodorizing, and decolorizing agents in various technological fields. In the food industry, sodium hypochlorite is the most widely used among chlorine compounds. The antimicrobial activity of a dilute hypochlorite solution is attributed largely to HOCl because of its cell membrane permeability. OCl<sup>-</sup> exhibits an excellent cleaning action for organic soils on solid surfaces. HOCl has been used as an aqueous solution, and its objects to be treated are things. In hypochlorite solution, HOCl is volatile and easily volatilized by stirring, bubbling, atomizing, or forced-air vaporization. On the other hand, OCl<sup>-</sup> is non-volatile and stays in the solution. Recently, the scope of objects to be treated with hypochlorite solution has been expanded to indoor spaces, and the use of gaseous hypochlorous acid (HOCl<sub>(g)</sub> ) has been studied intensively. This review describes the mechanisms of actions of hypochlorous acid as liquid-based and gaseous disinfectants and provides the evidence for the safety and effectiveness of HOCl<sub>(g)</sub> for controlling microorganisms in indoor spaces.</p>","PeriodicalId":73831,"journal":{"name":"Journal of microorganism control","volume":"28 4","pages":"165-175"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139486742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}