Advances in medical sciences最新文献

{"title":"Strengthening biobank networks: A Latvian case study within the StoP project","authors":"Evita Gašenko ,&nbsp;Ilona Freiliba ,&nbsp;Ilona Kojalo ,&nbsp;Arnis Kiršners ,&nbsp;Mārcis Leja","doi":"10.1016/j.advms.2026.03.007","DOIUrl":"10.1016/j.advms.2026.03.007","url":null,"abstract":"<div><h3>Purpose</h3><div>This publication aims to provide an overview of the challenges involved in establishing connections between national and international research teams and biobanks for the purposes of sample collection, preservation, and data analysis, using the Stomach Cancer Pooling (StoP) project as a case study.</div></div><div><h3>Materials and methods</h3><div>The StoP project illustrates collaboration in gastric cancer risk factor analysis. Internationally, the project integrates 33 research teams from 15 countries and associated biobanks to support the collection, preservation, and analysis of samples for epidemiological and genetic studies. Within Latvia, project activities involved the systematic collection of epidemiological, clinical, and biological data across multiple institutions. Latvian contributions were integrated into the broader StoP consortium to support comprehensive analyses.</div></div><div><h3>Results</h3><div>The collaborative effort has strengthened local biobanking infrastructure by expanding prospective data collection, updating standard operating procedures, and harmonizing retrospective datasets. It enhanced multi-institutional collaboration nationally and internationally. Key challenges were identified in data collection, harmonization, storage, funding, and sustainability. The StoP project working group has produced over forty scientific publications, with Latvian data contributing to five studies on smoking, <em>Helicobacter pylori</em> infection, sex-based differences, and proton pump inhibitor use. Current efforts focus on genome-wide association studies using collected biological specimens and epidemiological data.</div></div><div><h3>Conclusions</h3><div>The methods from the StoP project can be replicated across other institutions by focusing on robust ethical frameworks and data-sharing agreements. Effective cross-institutional collaboration further supports these efforts. Together, these practices increase public trust and enhance the scalability of such initiatives globally.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"71 1","pages":"Pages 138-146"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147600101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming growth factor-β1 downregulates tryptophanyl-tRNA synthetase expression in human lung fibroblasts","authors":"Aaron K. McDowell-Sanchez ,&nbsp;Konstantin Tsoyi","doi":"10.1016/j.advms.2026.02.002","DOIUrl":"10.1016/j.advms.2026.02.002","url":null,"abstract":"<div><h3>Purpose</h3><div>The role of tryptophanyl-tRNA synthetase, also known as WARS, in lung fibroblasts is currently unknown. We aimed to study the effect of transforming growth factor-beta 1 (TGF-β1) on the expression of WARS and whether it regulates profibrotic responses in TGF-β1-activated human lung fibroblasts.</div></div><div><h3>Materials and methods</h3><div>We used MRC-5, a human lung fibroblast cell line, and primary human lung fibroblasts (HLFs) derived from control subjects. WARS expression was measured by enzyme-linked immunosorbent assay (ELISA) and Western blot. Profibrotic responses in TGF-β1-stimulated human lung fibroblasts were measured by Western blot, gel contraction assay, and real-time quantitative PCR (RT-qPCR).</div></div><div><h3>Results</h3><div>We demonstrate that TGF-β1 potently downregulates WARS expression, both at the extracellular and intracellular levels, in MRC-5, and HLFs. Yin Yang 1 (YY1) transcription factor (TF) silencing ameliorates the inhibitory effect of TGF-β1 on WARS expression. Finally, we found that recombinant (r) WARS significantly inhibited fibronectin (FN) but had no effect on collagen1 (COL1) or alpha-smooth muscle actin (αSMA) expression in TGF-β1-induced HLFs.</div></div><div><h3>Conclusions</h3><div>Our results demonstrate that TGF-β1 inhibits WARS expression via YY1, however, WARS treatment has a modest effect on regulating profibrotic responses in activated HLFs.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"71 1","pages":"Pages 56-60"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146218257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intentional self-poisoning in children and adolescents 2018-2022: a single-center study with implications for suicide prevention","authors":"Przemysław M. Waszak ,&nbsp;Karena Dzwonnik ,&nbsp;Oliver Sowulewski ,&nbsp;Magdalena Dettlaff-Dunowska ,&nbsp;Paweł Zagożdżon","doi":"10.1016/j.advms.2026.02.005","DOIUrl":"10.1016/j.advms.2026.02.005","url":null,"abstract":"<div><h3>Purpose</h3><div>In recent years, the number of children and adolescent suicide attempts has increased in Poland. Intentional self-poisoning (ISP) is the most common method of committing suicide in that group. This study was designed to further analyze the trends and characteristics of pediatric emergency department (ED) presentations.</div></div><div><h3>Material and methods</h3><div>The study was a retrospective description of a case series (n = 113) from a single center, the regional pediatric ED. Data from &lt;18 years old patient reports from 2018 to 2022 were used for the analysis<strong>.</strong> The demographic data of patients, International Statistical Classification of Diseases (ICD) codes of diagnosis as well as drugs and their doses used for ISP were analyzed. Basic overall statistics and between-group comparisons were calculated with a p-significance level set on p &gt; 0.05.</div></div><div><h3>Results</h3><div>The average patient age was 14.4 years. Overdoses occurred most frequently at night, on Mondays, and in November/April. The most common psychiatric diagnosis was depressive episodes (45.13%). 52.0% of girls and 45.4% of boys were already under psychiatric care. Multiple drug overdoses accounted for 34.5%. The most frequently used substances were paracetamol (23.7%, mean dose 4150 mg), sertraline (13.8%, 1423 mg), and ibuprofen (10.5%, 8375 mg).</div></div><div><h3>Conclusions</h3><div>ISP among children and adolescents increased periodically during the COVID-19 pandemic, but it remains a significant problem for the healthcare system. Data shows over-the-counter painkillers, antipyretics, and psychiatric drugs were the primary substances used. Evidence-based prevention strategies that restrict access to these methods help reduce cases, and Poland should adopt them more strictly.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"71 1","pages":"Pages 61-67"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147321153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ToF-SIMS metabolomic analysis of urinary extracellular vesicles to identify potential markers for diabetic kidney disease in type 1 diabetes","authors":"Magdalena E. Skalska ,&nbsp;Agnieszka Kamińska ,&nbsp;Paulina Suduł ,&nbsp;Jakub Kurdziel ,&nbsp;Tomasz Klupa ,&nbsp;Maciej Małecki ,&nbsp;Ewa Ł. Stępień","doi":"10.1016/j.advms.2026.03.008","DOIUrl":"10.1016/j.advms.2026.03.008","url":null,"abstract":"<div><h3>Purpose</h3><div>Kidney damage is a significant concern in type 1 (T1DM) and type 2 (T2DM) diabetes mellitus, with diagnoses of chronic kidney disease (CKD) based on glomerular filtration rate and albumin excretion. An early marker for kidney damage is needed, and extracellular vesicles (EVs) show promise in this area. These nanostructures facilitate the transport of proteins, lipids, and nucleic acids between cells and undergo structural changes that can indicate the presence of disease.</div></div><div><h3>Materials and methods</h3><div>Our study employed time-of-flight secondary ion mass spectrometry (ToF-SIMS) to analyse lipid content in urinary EVs (uEVs) from 29 individuals with well-controlled T1DM (15 years duration, HbA1c ∼7%) and 16 healthy controls. uEVs were analysed using tunable resistive pulse sensing (TRPS) to determine size, concentration, and zeta potential, and by ToF-SIMS in both positive and negative ion modes to profile lipid- and amino acid-related ions.</div></div><div><h3>Results</h3><div>T1DM uEVs exhibited a more negative zeta potential (−27.65 ± 1.51 mV vs −18.43 ± 0.87 mV) and higher particle concentration ((7.1 ± 0.7) × 10¹¹ vs (6.4 ± 0.9) × 10¹⁰ particles/mL), while mean particle diameters were similar between groups. TOF-SIMS showed broadly comparable class-level proportions of lipids and amino acids; however, several individual lipid ions, including diacylglycerols, sphingolipids and selected fatty acids, displayed measurable differences. Amino-acid-related ions showed minor variations without major shifts in overall composition.</div></div><div><h3>Conclusions</h3><div>These findings suggest that uEVs may reflect pathological processes in individuals with T1DM without CKD. However, further research is necessary to confirm whether uEVs can be markers for CKD.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"71 1","pages":"Pages 147-158"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics of hospitalized patients with hepatitis E virus infection in Poland: a multi-centre retrospective study","authors":"Anna Parfieniuk-Kowerda ,&nbsp;Jerzy Jaroszewicz ,&nbsp;Piotr Grabarczyk ,&nbsp;Dorota Zarebska-Michaluk ,&nbsp;Katarzyna Sikorska ,&nbsp;Anna Piekarska ,&nbsp;Aleksandra Berkan-Kawinska ,&nbsp;Dorota Kozielewicz ,&nbsp;Małgorzata Pawlowska ,&nbsp;Monika Pazgan-Simon ,&nbsp;Barbara Sobala-Szczygiel ,&nbsp;Piotr Zielinski ,&nbsp;Andrzej Rydzewski ,&nbsp;Agnieszka Serwacka ,&nbsp;Krzysztof Tomasiewicz ,&nbsp;Bozena Katarzyna Budziszewska ,&nbsp;Robert Flisiak","doi":"10.1016/j.advms.2026.02.003","DOIUrl":"10.1016/j.advms.2026.02.003","url":null,"abstract":"<div><h3>Purpose</h3><div>The burden of hepatitis E virus (HEV) infections in Poland is highly underestimated and in contrast with high anti-HEV IgG seroprevalence in Polish population. This is the first publication addressing the issues of HEV infections in Poland as well as surveillance and reporting problems referring to this particular disease.</div></div><div><h3>Patients and methods</h3><div>In this retrospective study we analysed 61 HEV cases referred to and diagnosed in 10 leading centres in Poland.</div></div><div><h3>Results</h3><div>Males constituted 70.5% of the study group, with equal distribution of cases below and over 50 years of age. Comorbidities were reported in 73.8% of this group. Of major concern was the high frequency of reported complications and clinical outcomes: 3 cases of acute liver failure (ALF) with 1 resulting in orthotopic liver transplantation (OLTx), 1 case of renal insufficiency, 3 cases of neuralgic amyotrophy, 8 cases of thromocytopaenia, and 4 cases of chronic hepatitis E.</div></div><div><h3>Conclusions</h3><div>The HEV infections in Poland are considerably underestimated, which results both from the lack of awareness of general practitioners and limited access to HEV diagnostics, particularly in primary and secondary care medical facilities in Poland.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"71 1","pages":"Pages 75-82"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147300601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of FKBP5 gene polymorphism with insomnia symptoms in the depressive episodes of stress-related bipolar disorder","authors":"Ovinuchi Ejiohuo ,&nbsp;Karolina Bilska ,&nbsp;Karolina Gattner ,&nbsp;Łukasz Mech ,&nbsp;Dominik Spalek ,&nbsp;Maria Skibińska ,&nbsp;Monika Dmitrzak-Węglarz ,&nbsp;Paweł Kapelski ,&nbsp;Beata Narożna ,&nbsp;Bartłomiej Budziński ,&nbsp;Aleksandra Szczepankiewicz ,&nbsp;Joanna Pawlak","doi":"10.1016/j.advms.2025.09.004","DOIUrl":"10.1016/j.advms.2025.09.004","url":null,"abstract":"<div><h3>Purpose</h3><div>Stress-related bipolar disorder (BP) arises from complex genetic, environmental, and clinical interactions. While <em>FKBP5</em>(key stress response regulator) is linked to mood disorders, its role in insomnia during depressive episodes remains unclear. This study explores the association between <em>FKBP5</em> polymorphisms and insomnia symptoms occurring during the depressive episodes of BP.</div></div><div><h3>Materials and methods</h3><div>A total of 347 individuals with BP (42 ​% male, 58 ​% female; 78 ​% insomnia symptoms) were assessed using the SCID, OPCRIT, and TaqMan genotyping for eight <em>FKBP5</em> polymorphisms. Participants were grouped based on the presence/absence of a stressor before the first episode. Statistical analyses (ANCOVA and Chi-square with pairwise post hoc tests) were performed using Statistica 13.3 and R. Functional annotation of significantly associated variants was conducted using Ensembl VEP, RegulomeDB, HaploReg, and SNPnexus.</div></div><div><h3>Results</h3><div><em>FKBP5</em> rs755658 polymorphism may be associated with insomnia symptoms in individuals with stressors, with CT/CC genotypes showing a higher likelihood of symptoms (<em>p</em> ​= ​0.03; BH-adjusted <em>p</em> ​= ​0.22, below the 0.25 threshold) compared to the TT. Seven polymorphisms were significantly associated with BP subtypes in those without stressors, suggesting genetic influence. The rs755658 variant is predicted to affect gene expression, transcription factor binding, and post-transcriptional regulation.</div></div><div><h3>Conclusions</h3><div>These findings suggest that <em>FKBP5</em> genetic variants may affect sleep disturbances with a stressor present, highlighting their role in stress regulation and insomnia during depressive episodes in BP. Given the study's exploratory nature, findings should be interpreted cautiously and validated in larger, independent samples. Future studies should investigate the underlying mechanisms and therapeutic implications.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 335-345"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145051300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of the C2HEST score in predicting the clinical outcomes of COVID-19 in obese and non-obese cohorts - subanalysis of the COLOS Study","authors":"Piotr Rola ,&nbsp;Olgierd Dróżdż ,&nbsp;Adrian Doroszko ,&nbsp;Małgorzata Trocha ,&nbsp;Krzysztof Kujawa ,&nbsp;Agnieszka Bronowicka-Szydełko ,&nbsp;Agnieszka Matera-Witkiewicz ,&nbsp;Dorota Bednarska-Chabowska ,&nbsp;Maciej Rabczyński ,&nbsp;Edwin Kuźnik ,&nbsp;Marcin Madziarski ,&nbsp;Jędrzej Machowiak ,&nbsp;Rafał Małecki ,&nbsp;Michał Tkaczyszyn ,&nbsp;Joanna Adamiec-Mroczek ,&nbsp;Janusz Sokołowski ,&nbsp;Jarosław Nowak ,&nbsp;Ewa A. Jankowska ,&nbsp;Katarzyna Madziarska","doi":"10.1016/j.advms.2025.08.002","DOIUrl":"10.1016/j.advms.2025.08.002","url":null,"abstract":"<div><h3>Purpose</h3><div>Cardiovascular risk factors, particularly obesity, are strong predictors of poor COVID-19 outcomes, though prognosis remains uncertain for overweight individuals. Identifying new tools to assess obesity's impact on COVID-19 severity is vital for early risk stratification. We evaluated the C₂HEST-score system's ability to predict unfavorable outcomes in hospitalized COVID-19 patients.</div></div><div><h3>Material and methods</h3><div>Consecutive 2183 patients hospitalized due to confirmed COVID-19 were enrolled to this study. Based on the physical examination and past medical history, 332 carefully selected patients were assigned to one of two of the study cohorts, i.e. obese (n ​= ​191) vs. non-obese (n ​= ​141), and allocated to different risk-strata based on the C₂HEST score result.</div></div><div><h3>Results</h3><div>A total of 52 in-hospital deaths (27.2 ​%) were reported in the obese cohort, while in the non-obese cohort the in-hospital mortality rate was 17 ​% (24 reported cases). The in-hospital, 3-month and 6-month mortality were the highest in the high-risk C₂HEST stratum in both study cohorts, reaching respectively 47.62 ​%, 57.14 ​%, and 57.14 ​% in the obese and 63.64 ​%, 72.73 ​%, and 88.89 ​% in the non-obese cohorts. Among patients with the high-risk C₂HEST-score, non-obese individuals were more likely to experience myocardial injury and acute heart failure, whereas obese individuals were more prone to develop acute kidney injury.</div></div><div><h3>Conclusions</h3><div>This study shows the usefulness and performance of the C₂HEST-score in predicting the adverse outcomes of COVID-19 in hospitalized patients with obesity. In the cohort with normal body mass, the C₂HEST score revealed to be far more precise in the risk prediction.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 318-325"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and clinical associations of antinuclear antibodies (ANA) in spondyloarthropathies","authors":"Adrianna Błahuszewska-Omyła ,&nbsp;Emilia Frąckiel ,&nbsp;Marek Bielecki ,&nbsp;Ewa Gińdzieńska- Sieśkiewicz ,&nbsp;Natalia Szymańska ,&nbsp;Otylia Kowal-Bielecka","doi":"10.1016/j.advms.2025.09.005","DOIUrl":"10.1016/j.advms.2025.09.005","url":null,"abstract":"<div><h3>Purpose</h3><div>Spondyloarthropathies (SpA) are a broad and diverse group of diseases with highly variable clinical manifestations. Due to this wide range of symptoms and their frequent overlap with other disease entities, SpA present numerous diagnostic and therapeutic challenges. Moreover, the pathogenesis of SpA remains unexplained. While the currently prevailing hypothesis suggests an autoinflammatory mechanism, the involvement of autoimmune processes has not been ruled out. To explore this possibility, the present study aimed to assess the presence of antinuclear antibodies (ANA) and their clinical associations in patients with SpA.</div></div><div><h3>Patients and methods</h3><div>The analysis included 117 consecutive patients with different forms of SpA, i.e., ankylosing spondylitis (n ​= ​52), psoriatic arthritis (n ​= ​34), and non-radiographic SpA (n ​= ​31). They underwent clinical evaluation, and serum samples were collected from each for ANA testing using indirect immunofluorescence.</div></div><div><h3>Results</h3><div>ANA were found in 46 ​% of patients with SpA. No statistically significant association was found between ANA and diagnosis of specific SpA or clinical symptoms. Interestingly, the highest prevalence of ANA (63 ​%) was found in women with non-radiographic spondyloarthropathy and the lowest (38 ​%) in males with ankylosing spondylitis, however, the difference did not reach statistical significance.</div></div><div><h3>Conclusion</h3><div>The results of our study suggest that ANA are frequent in patients with SpA, in particular in women with early, non-radiographic SpA which may be of clinical significance. However, further studies, involving larger populations, are necessary to clarify the significance of ANA presence in SpA patients.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 377-381"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the effects of umbilical cord and adipose-derived mesenchymal stem cells on endoplasmic reticulum stress in cadmium-induced rat kidney","authors":"Bahar Kartal ,&nbsp;Uygar Saçik ,&nbsp;Güven Erbil","doi":"10.1016/j.advms.2025.07.002","DOIUrl":"10.1016/j.advms.2025.07.002","url":null,"abstract":"<div><h3>Purpose</h3><div>The hazardous heavy metal cadmium (Cd) has the potential to cause long-term kidney damage, mostly dependent on autophagy. Endoplasmic reticulum (ER) stress has been recognized as a primary source of Cd-induced toxicity. The ER chaperone GRP78 binds ER stress sensors, keeping them dormant. Exposure to Cd increases ER stress, a well-known inducer of autophagy. Adipose-derived mesenchymal stem cells (AD-MSC) are potentially useful tissue engineering and cellular treatment tools. Various disorders are treated with human umbilical cord MSCs (HUC-MSCs). They possess several unique qualities that are necessary for their therapeutic uses. The study aimed to investigate the effects of AD-MSCs and HUC-MSCs on Cd-induced nephrotoxicity.</div></div><div><h3>Methods</h3><div>The study used 36 male Wistar albino rats that were divided into six groups: control, AD-MSC, HUC-MSC, Cd, Cd ​+ ​AD-MSC, and Cd ​+ ​HUC-MSC. Hematoxylin and eosin (H&amp;E) were used to stain the renal tissues in preparation for a histological analysis. Furthermore, the ER stress level was assessed by measuring GRP78 immunoexpression. Additionally, LC3B and Beclin-1 immunostaining were used to determine the autophagy.</div></div><div><h3>Results</h3><div>The histopathological results showed that the glomerular structure, proximal and distal tubules were disrupted in rat kidneys from the Cd group. Treatment with AD-MSCs and HUC-MSCs restored renal histological damage caused by Cd. Additionally, in Cd-induced renal tissues, there was an increase in the immunoexpression of the autophagic sensors LC3B and Beclin-1 and the ER stress indicator GRP78.</div></div><div><h3>Conclusion</h3><div>MSCs enabled Cd-damaged kidney tissues to regain an almost healthy histological structure.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 284-290"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transition from intravenous to subcutaneous infliximab in a cohort of patients with inflammatory bowel disease: results of the INESTIMABLE retrospective observational study","authors":"Alessia Todeschini ,&nbsp;Andrea Geccherle ,&nbsp;Paola Signoretto ,&nbsp;Nicoletta Colaci ,&nbsp;Federica Mellone ,&nbsp;Nicoletta De Santis ,&nbsp;Massimo Guerriero ,&nbsp;Giuliano Barugola ,&nbsp;Angela Variola","doi":"10.1016/j.advms.2025.10.001","DOIUrl":"10.1016/j.advms.2025.10.001","url":null,"abstract":"<div><h3>Purpose</h3><div>This study aimed to present real-world data regarding the treatment of inflammatory bowel disease (IBD) with the subcutaneous (SC) formulation of the tumor necrosis factor inhibitor, infliximab.</div></div><div><h3>Methods</h3><div>Adult IBD patients who switched from intravenous (IV) infliximab to SC infliximab were recruited for this study. Data on the efficacy of maintaining long-term remission, persistence in therapy and the safety profile were obtained from medical records and physician assessments conducted during routine clinical practice. Data on adherence to treatment schedules and patient satisfaction were collected through an <em>ad hoc</em> questionnaire. The duration of the follow-up was 12 months.</div></div><div><h3>Results</h3><div>The treatment with SC infliximab was generally well-tolerated, with a persistence rate of 86.7 ​% at 12 months and adherence to the treatment schedule of 86.4 ​%. Patients reported a favorable experience with self-injections. Mild, self-limiting adverse events were observed, and inflammatory biomarkers decreased over time, suggesting maintenance of remission in most patients.</div></div><div><h3>Conclusions</h3><div>The findings suggest that SC infliximab may be a viable option for IBD patients in remission who are eligible for switching, offering a self-administered alternative to IV therapy.</div></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"70 2","pages":"Pages 392-400"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}