Advances in Clinical and Experimental Medicine最新文献

{"title":"Protective effect of clotrimazole on lung injury in an experimental model of ruptured abdominal aortic aneurysm.","authors":"Zerrin Pulathan, Şaban Murat Ergene, Gökalp Altun, Esin Yuluğ, Ahmet Menteşe","doi":"10.17219/acem/182821","DOIUrl":"10.17219/acem/182821","url":null,"abstract":"<p><strong>Background: </strong>Lungs are the target organs most affected by ischemia/reperfusion (I/R) injury, which is exacerbated when hemorrhagic shock occurs. Suppressing various proinflammatory cytokines, inflammation and oxidation that initiate and aggravate lung damage with various drugs or methods provides significant benefits in preventing lung damage.</p><p><strong>Objectives: </strong>This study aims to evaluate the protective effect of clotrimazole (CLT), an antimycotic drug, on lung injury and systemic inflammatory response in rats by creating an experimental model of a ruptured abdominal aortic aneurysm (RAAA).</p><p><strong>Material and methods: </strong>Thirty-six male Sprague Dawley rats were randomly divided into 5 groups: sham, sham+CLT, sham+polyethylene glycol (PEG), shock+ischemia/reperfusion (SIR), and SIR+CLT. Saline, CLT and PEG were administered in the sham groups without shock and I/R. The hemorrhagic shock was developed in SIR groups by drawing blood for 1 h to keep the mean arterial pressure at 50 mm Hg. After 60 min, the SIR+CLT group was given 20 mg/kg CLT; then, the aortic clamps were opened, and rats were left for 120 min of reperfusion. The blood taken to create hemorrhagic shock was returned in a controlled manner during this time. At the end of the reperfusion procedure, samples were taken for cytokine levels in serum and lung tissue and for other biochemical analyses. Blood gas, histopathological examination and wet/dry weight measurements were performed to assess lung injury.</p><p><strong>Results: </strong>An increase was observed in all parameters in the SIR group compared to the sham group. In the SIR+CLT group, the serum myeloperoxidase (MPO), tumor necrosis factor alpha (TNF-α), lung MPO values, histologically lung injury scores, and lung tissue wet/dry ratio were decreased significantly when compared to the SIR group (p < 0.05).</p><p><strong>Conclusions: </strong>These results indicate that CLT may reduce the systemic inflammatory response and lung injury due to shock and I/R in an experimental model of RAAA.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"91-100"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139970585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between skin lymphangiogenesis parameters and arterial hypertension status in patients: An observational study.","authors":"Angelika Chachaj, Ivana Stanimirova, Mariusz Chabowski, Agnieszka Gomułkiewicz, Paweł Hodurek, Natalia Glatzel-Plucińska, Mateusz Olbromski, Aleksandra Piotrowska, Aleksandra Kuzan, Jędrzej Grzegrzółka, Katarzyna Ratajczak-Wielgomas, Aleksandra Nowak, Ewa Szahidewicz-Krupska, Jerzy Wiśniewski, Mariusz A Bromke, Marzenna Podhorska-Okołów, Andrzej Gamian, Dariusz Janczak, Piotr Dzięgiel, Andrzej Szuba","doi":"10.17219/acem/184060","DOIUrl":"10.17219/acem/184060","url":null,"abstract":"<p><strong>Background: </strong>Recent studies have indicated that the skin lymphatic system and interstitium may play a role in the pathophysiology of arterial hypertension (AH).</p><p><strong>Objectives: </strong>We aimed to determine whether the set of pathway parameters described previously in rodents would allow for the distinction between hypertensive and normotensive patients.</p><p><strong>Material and methods: </strong>Molecular and histopathological parameters from the skin and blood of patients with AH (AH group, n = 53), resistant AH (RAH group, n = 32) and control (C group, n = 45) were used, and a statistical multivariate bootstrap methodology combining partial least squares-discriminant analysis (PLS-DA) and selectivity ratio (SR) were applied.</p><p><strong>Results: </strong>The C vs RAH model presented the best prediction performance (AUC test = 0.90) and had a sensitivity and specificity of 73.68% and 83.33%, respectively. However, the parameters selected for the C vs AH group model were the most important for the pathway described in the rodent model, i.e., greater density of the skin lymphatic vessels (D2-40 expression) and greater number of macrophages (CD68 expression), higher expression of the messenger ribonucleic acid (mRNA) of nuclear factor of activated T cells 5 (NFAT5), vascular endothelial growth factor C (VEGFC) and podoplanin (PDPN) in the skin, greater concentration of hyaluronic acid (HA) in the skin, and lower serum concentration of VEGF-C.</p><p><strong>Conclusions: </strong>Our study suggests that the NFAT5/VEGF-C/lymphangiogenesis pathway, previously described in rodent studies, may also be present in human HA. Further experiments are needed to confirm our findings.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"63-73"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140179042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression of miRNA-769-5p and Smad2 in patients with or without oral cGVHD.","authors":"Xiang-Zhi Yong, Yu-Xi Zhou, Tian-Tian Wu, Qiao-Zhi Jiang, Zhen-Min Liu, Zhong-Ming Zhang, Rong-Quan He, Zhi-Guang Huang, Gang Chen, Renchuan Tao","doi":"10.17219/acem/181147","DOIUrl":"10.17219/acem/181147","url":null,"abstract":"<p><strong>Background: </strong>Oral chronic graft-versus-host disease (cGVHD) impacts quality of life of patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, its precise pathogenesis remains unknown, with potential associations with differential microRNA (miRNA) expression and the TGF-â/Smad signaling pathway.</p><p><strong>Objectives: </strong>This study aims to explore miRNA expression profiles in the peripheral blood of oral cGVHD patients, focusing on miRNA-769-5p and its relationship with Smad2.</p><p><strong>Material and methods: </strong>Peripheral venous blood samples were collected for RNA extraction from 8 patients with oral cGVHD, 8 patients without cGVHD and 8 participants from the healthy control group. The miRNA library was constructed using the Illumina Hiseq 2500 platform. We focused on identifying miRNAs associated with the TGF-â/Smad signaling pathway and subsequently conducted validation experiments. The oral cGVHD and without cGVHD groups were each expanded to include 15 individuals. Peripheral blood samples were subjected to polymerase chain reaction (PCR) analysis to assess miRNA levels and to evaluate Smad2 mRNA levels in peripheral blood mononuclear cells (PBMC). Additionally, enzyme-linked immunosorbent assay (ELISA) was conducted to determine the Smad2 protein levels in peripheral blood.</p><p><strong>Results: </strong>The most significantly differentially expressed miRNAs among the 3 groups were miRNA-505-5p and miRNA-769-5p. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated an enrichment of the target genes of miRNA-769-5p in the TGF-â signaling pathway. It was observed that miRNA-769-5p expression was higher in patients without oral cGVHD in comparison to those with oral cGVHD. Receiver operating characteristic (ROC) analysis demonstrated that miRNA-769-5p holds diagnostic value for oral cGVHD. As a target of miRNA-769-5p, Smad2 mRNA exhibited a negative correlation with it. Moreover, both Smad2 mRNA and protein levels were higher in patients with oral cGVHD as opposed to those without cGVHD.</p><p><strong>Conclusions: </strong>Differential expression of miRNAs, particularly the downregulation of miRNA-769-5p, may influence the development of oral cGVHD by diminishing its inhibitory effect on the TGF-â/Smad signaling pathway through its interaction with Smad2.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"53-62"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating gender dynamics in forensic toxicology: The role of masculinity and femininity in alcohol and drug abuse.","authors":"Arianna Giorgetti, Rafael Boscolo-Berto","doi":"10.17219/acem/199712","DOIUrl":"10.17219/acem/199712","url":null,"abstract":"<p><p>The concept of \"gender\" refers to the socially constructed characteristics that define feminine or masculine behavior, which are constantly changing and can influence access to healthcare and patterns of help-seeking. These factors significantly impact forensic toxicology, a key area within the medicolegal landscape, forcing the adoption of a gender-sensitive approach to better understand the differing impacts of substances on men and women. Research indicates significant disparities in drug use between genders; men are more likely to abuse alcohol and illicit drugs, while women tend to use prescription medications. Although men typically show higher rates of driving under influence (DUI) related to alcohol, significant alcohol-related DUI cases also exist among women. In postmortem toxicology, gender affects drug pharmacokinetics and pharmacodynamics, with a pressing need for more research focused on women's specific toxic and fatal ranges. The rise of new psychoactive substances (NPS) presents additional challenges; while most users are male, the gender gap appears to be narrowing. Further investigation into the gender differences in drug usage and effects, particularly regarding NPS, is essential for improving justice system responses and healthcare delivery. A gender-based approach in forensic toxicology is vital for addressing these issues effectively.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"5-8"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bergapten attenuates human papillary thyroid cancer cell proliferation by triggering apoptosis and the GSK-3β, P13K and AKT pathways.","authors":"Jingjing Li, Shaik A Hussain, Jayasimha R Daddam, Mengjie Sun","doi":"10.17219/acem/183877","DOIUrl":"10.17219/acem/183877","url":null,"abstract":"<p><strong>Background: </strong>Over the past few decades, thyroid cancer (TC) incidence has steadily increased globally. The most common TC is human papillary thyroid carcinoma (PTC), which is poorly responsive to the current treatments. Hence, finding a successful therapeutic is urgently required.</p><p><strong>Objectives: </strong>Bergapten (BG) is a furanocoumarin, a natural psoralen derivative isolated from numerous species of citrus and bergamot oil that has demonstrated anti-tumor activity. However, there are no reports available on the efficacy of BG on PTC cells.</p><p><strong>Material and methods: </strong>The current research investigated the anti-cancer activity of BG on human BCPAP cells, with cytotoxicity and apoptosis evaluated using MTT assay, AO/EB, DAPI, PI, ELISA, mRNA, and western blot.</p><p><strong>Results: </strong>Bergapten (control group, 10 μM/mL and 15 μM/mL) inhibited PTC cell proliferation and stimulated apoptosis by enhancing Bax and caspase and reducing Bcl-2, cyclin-D1, c-myc, and survivin in a dose-dependent manner. Furthermore, BG expressively attenuated PI3K/AKT/GSK-3β signaling, creating an uneven Bax/Bcl-2 ratio that triggered Cyt-c, caspase cascade and apoptosis in human PTC cells.</p><p><strong>Conclusions: </strong>Our findings emphasize that BG has the potential to be used as a protective natural remedy for human PTC cells.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"113-122"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival benefits of gastrectomy in patients with metastatic gastric cancer: A meta-analysis.","authors":"Lifan Chen, Yu Chen","doi":"10.17219/acem/184268","DOIUrl":"10.17219/acem/184268","url":null,"abstract":"<p><strong>Background: </strong>Individuals with metastatic gastric cancer (MGC) are incurable and have a poor prognosis. To date, surgical resection with curative intent is the only treatment providing hope for a cure, but the role of surgical resection is still controversial.</p><p><strong>Objectives: </strong>To assess the effects of gastrectomy compared to non-resection on MGC patient survival.</p><p><strong>Material and methods: </strong>PubMed, Embase, Cochrane Library, and Web of Science databases were searched up to October 10, 2023. Primary outcomes were 1-, 2-, 3-, and 5-year overall survival (OS), OS, and OS time.</p><p><strong>Results: </strong>Forty-six studies with 7,152 MGC patients were included. Compared to MGC patients receiving no resection, MGC patients with gastrectomy had significantly improved 1-year OS (pooled relative risk (RR):1.90, 95% confidence intervals (95% CIs): 1.50, 2.41), 2-year OS (pooled RR: 2.23, 95% CI: 1.40, 3.53), 3-year OS (pooled RR: 6.09, 95% CI: 3.12, 11.87), 5-year OS (pooled RR: 4.30, 95% CI: 1.35, 13.74), and reduced risk of death (pooled hazard ratio (HR): 0.49, 95% CI: 0.37, 0.65). Gastrectomy combined with metastasectomy or not also revealed similar results regarding OS and risk of death. Additionally, OS time was significantly longer in patients receiving gastrectomy than patients not receiving resection (pooled weighted mean difference (WMD): 6.06, 95% CI: 1.36, 10.760). No significant difference in postoperative morbidity was detected between the patients receiving gastrectomy and patients not receiving resection (pooled RR: 2.54, 95% CI: 0.13, 51.39).</p><p><strong>Conclusion: </strong>Gastrectomy, with or metastasectomy, may provide MGC patients with survival benefits.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"9-23"},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of the structure of the masticatory system on the presence and severity of the gag reflex in children with cerebral palsy.","authors":"Roksana Malak, Oskar Komisarek, Karolina Biel, Karolina Szuflak, Katarzyna Wiecheć, Tomasz Szczapa, Joanna Kasperkowicz, Maja Matthews-Kozanecka, Teresa Matthews-Brzozowska, Magdalena Kobylińska, Jacek Kwiatkowski, Włodzimierz Samborski, Ewa Mojs","doi":"10.17219/acem/189851","DOIUrl":"10.17219/acem/189851","url":null,"abstract":"<p><strong>Background: </strong>Dysphagia, prevalent in 90% of children with neurological disorders, poses risks of medical complications and is associated with cognitive and psychosocial challenges. The absence of the sucking-swallowing reflex and variations in the gag reflex contribute to feeding difficulties.</p><p><strong>Objectives: </strong>This study focuses on examining the impact of the gag reflex on the masticatory system structure in children with cerebral palsy, aiming to assess its significance.</p><p><strong>Material and methods: </strong>This observational study investigated the gag reflex and soft palate shape in 25 children with cerebral palsy (average age: 14 years). Inclusion criteria considered specific levels of the Eating and Drinking Ability Classification System (EDACS) and the Gross Motor Function Classification System (GMFCS). Exclusion criteria comprised hypotension, inflammation and tumors. The Castillo-Morales questionnaire assessed variables and statistical analysis (Spearman's rank correlation and non-parametric tests) utilizing PQStat v. 1.8.6.120 software.</p><p><strong>Results: </strong>Findings did not reveal an association between the absence of the gag reflex and abnormal palate structure in children. Our results showed a correlation between higher tension of the buccinator muscles and mobility of the tongue on the structure of the palate.</p><p><strong>Conclusions: </strong>Children with neurological disorders, such as cerebral palsy, are a diverse group requiring specialized orthodontic treatment and close interdisciplinary collaboration.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective use of miRNAs as biomarkers in the diagnosis of Alzheimer's disease.","authors":"Agata Haśko, Natalia Potocka, Marzena Skrzypa, Halina Bartosik-Psujek, Izabela Zawlik","doi":"10.17219/acem/190273","DOIUrl":"https://doi.org/10.17219/acem/190273","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is the leading cause of dementia in the aging population. Pathogenic processes related to the accumulation of amyloid plaques (Aβ) and intracellular neurofibrillary tangles (NFTs) begin during the asymptomatic stage long before the onset of deterioration in cognitive functions and neurodegeneration, which makes rapid diagnosis and treatment difficult. Although biochemical diagnostic markers isolated from the body fluids of AD patients are currently used, scientists are engaged in research into molecular biomarkers that will significantly accelerate the diagnosis long before the first clinical symptoms appear. The research presented here focused on microRNAs (miRNAs), small, non-coding RNA molecules that are involved in the regulation of the post-transcriptional expression of many genes. A review of the literature revealed that miRNAs play an important role in regulating the expression of genes involved in the pathophysiological mechanisms of AD. Changes in the levels of miRNAs in a patient's body fluids can be used for rapid diagnosis. Original scientific articles published between 2014 and 2023 describing clinical and experimental studies on the role and expression levels of various miRNAs were selected from scientific databases such as PubMed, NCBI, Science Direct, and Google Scholar. The selected miRNAs were divided into 2 groups based on their expression level in AD: those with increased expression and those with decreased expression. A review of the latest scientific reports confirms that miRNAs may be a promising source of non-invasive and widely available biomarkers. Additionally, their modulation may prove to be an effective therapeutic strategy in AD.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 health communication strategies for older adults: Chatbots and traditional media.","authors":"Robert Olszewski, Klaudia M Watros, Jakub Brzeziński, Jakub Owoc, Małgorzata Mańczak, Tomasz Targowski, Krzysztof Jeziorski","doi":"10.17219/acem/195242","DOIUrl":"https://doi.org/10.17219/acem/195242","url":null,"abstract":"<p><strong>Background: </strong>The coronavirus disease 2019 (COVID-19) pandemic has significantly accelerated the development and use of new healthcare technologies. While younger individuals may have been able to quickly embrace virtual advancements, older adults may still have different needs in terms of health communication.</p><p><strong>Objectives: </strong>To identify areas of interest and preferred sources of information related to the COVID-19 pandemic among older adults and to verify their eHealth competencies.</p><p><strong>Material and methods: </strong>The study was conducted between February 2022 and July 2022. It included listeners from the University of the Third Age (U3A) and younger students. Both groups received information about the HealthBuddy+ chatbot, a questionnaire that addressed respondents' interests about COVID-19, and the PL-eHEALS (eHealth Literacy Scale) questionnaire to measure their eHealth competencies.</p><p><strong>Results: </strong>There were 573 participants in the study (U3A listeners - 303 participants, median age: 73 years (interquartile range (IQR): 69-77); young adult students - 270, median age: 24 years (IQR: 23-24). The primary source of information about COVID-19 for older adults was television (84.5%), and for younger adults, internet (84.4%). Among the older adults, only 17% ever interacted with a chatbot (younger adults - 78% respectively), and 19% considered it a trustworthy source of information on COVID-19 compared to 79% of younger respondents. Older adults and younger adults in our study were most interested in COVID-19 treatment methods (45.5% and 69.3%, respectively), symptoms of the disease (36.6% and 35.2%, respectively) and chronic diseases coexisting with COVID-19 (35.0% and 51.5%, respectively). However, their eHealth competencies were generally low (median (Me): 34; IQR: 30-39) compared to younger adults (Me: 42; IQR: 40-47).</p><p><strong>Conclusions: </strong>Health education for older adults should be appropriately tailored to their current needs and differentiated. The level of eHealth competencies of older adults suggests that much work remains to narrow the gap between the eHealth competencies of the younger and older generations.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiRNA in archival serum samples derived from breast cancer patients treated with neoadjuvant chemotherapy vs freshly collected samples: Pilot study.","authors":"Marcin Kubeczko, Patrycja Tudrej, Tomasz Tyszkiewicz, Aleksandra Krzywon, Małgorzata Oczko-Wojciechowska, Michał Jarząb","doi":"10.17219/acem/193265","DOIUrl":"https://doi.org/10.17219/acem/193265","url":null,"abstract":"<p><strong>Background: </strong>Liquid biopsy, including miRNA profiling, is a promising approach to identify breast cancer (BC) resistance. However, the effect of long-term storage on the quality of miRNA assessment in archival serum has not been fully addressed.</p><p><strong>Objectives: </strong>We aimed to determine whether miRNAs were recoverable from long-stored serum samples to subsequently evaluate prognostic and predictive miRNA value in the archival collection of samples from patients treated with neoadjuvant chemotherapy at Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice, Poland.</p><p><strong>Material and methods: </strong>We have evaluated miRNA quantity in serum samples stored for up to 12 years. Additionally, we compared miRNA expression in archival samples to freshly collected samples derived from advanced BC patients.</p><p><strong>Results: </strong>Forty BC patients were included in the study. Archival samples were derived from 20 BC patients treated with radical intent between 2011 and 2015. Freshly collected samples were collected from 20 advanced BC patients in 2022. miRNA was present in archived serum samples frozen at -80C° for at least 12 years. Additionally, we found significantly different expressions between the 2 analyzed groups. Expression of circulating miR-16, -17, -18a, -20a, -21, -27a, -30b, -222, and -326 were significantly higher in archival samples, whereas expression of circulating miR-19a, -29b, -29c, -128, -145, -146a, -193b, -195, -200b, -210, -221, -424, and -451a were lower than in freshly collected samples. In 14 miRs, we observed expression in both groups; however, differences were statistically insignificant (miR-1, -7a, -10b, -19b, -34a, -99a, -106b, -122, -125b, -155, -200a, -205, -223, -340).</p><p><strong>Conclusions: </strong>MiRNA can be identified from long-stored samples, making large prospectively collected serum repositories with long follow-up time an invaluable source for miRNA biomarker discovery.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}