Advances in Clinical and Experimental Medicine最新文献

{"title":"Next-generation sequencing study of inflammatory spindle cell lesions focused on receptor tyrosine kinase gene rearrangements most frequently occurring in inflammatory myofibroblastic tumor.","authors":"Krzysztof Siemion, Joanna Kiśluk, Natalia Wasilewska, Joanna Reszec-Gielażyn, Anna Korzyńska, Tomasz Łysoń, Zenon Mariak","doi":"10.17219/acem/203097","DOIUrl":"https://doi.org/10.17219/acem/203097","url":null,"abstract":"<p><strong>Background: </strong>A group of inflammatory spindle cell lesions (ISCLs) includes many nosological entities with a common histological image consisting of spindle-shaped cells and inflammatory infiltrate. Diverse diseases indicate different prognoses that can be difficult to predict. The most well-known neoplasm from the group is an inflammatory myofibroblastic tumor (IMT) that harbors tyrosine kinase gene rearrangement frequently affecting ALK, ROS1, RET, PDGFRB, NTRK, and IGF1R genes. In contrast, a reactive mass-forming lesion is regarded as an inflammatory pseudotumor (IPT).</p><p><strong>Objectives: </strong>This study aimed to: 1) investigate the accuracy of the primary diagnosis of IMT and IPT with the diagnostics using extended analysis of clinical data, re-evaluation of histopathological slides and next-generation sequencing (NGS); and 2) to establish prognostic and diagnostic factors.</p><p><strong>Material and methods: </strong>Finally, 46 cases of ISCLs were retrieved. The authors revised diagnoses and performed NGS based on ribonucleic acids isolated from selected paraffin blocks. Clinical and paraclinical data were also collected. The final diagnoses were made as a result of available information integration.</p><p><strong>Results: </strong>The sequencing confirmed 4 IMTs and detected 4 fusion gene types - EML4-ALK, RANBP2-ALK, and ETV6-NTRK3. Additionally, 1 afunctional EGFR-PPARGC1A rearrangement was found in gastric inflammatory fibroid polyp. A subset of reactive lesions also contained some mutations, which is consistent with actual knowledge. Neoplasms with ganglion-like cells, nuclear atypia and increased mitotic activity gave local recurrences. A higher percentage of necrosis indicated IMTs and patients who died in the analyzed period. No relation between genetic alterations and relapse was found.</p><p><strong>Conclusions: </strong>A final diagnosis can be made based on all clinical and paraclinical data. The prognosis after the treatment is dependent on the pathological diagnosis, disease location and resection completeness, presence of ganglion-like cells, nuclear atypia, mitotic index, and necrosis. Not only neoplastic but also reactive lesions can recur. The presence of gene rearrangements and necrosis can have diagnostic value.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstruction of outer glycolipid synthesis pathways from Porphyromonas gingivalis in Escherichia coli for production of a vaccine candidate.","authors":"Ewa Brzozowska, Wiesław Świętnicki, Jordan Sycz, Monika Kołodziejczak, Łukasz Stachowicz, Anna Wzorek, Agnieszka Korzeniowska-Kowal, Michał Skowicki, Tomasz Lipiński","doi":"10.17219/acem/200882","DOIUrl":"https://doi.org/10.17219/acem/200882","url":null,"abstract":"<p><strong>Background: </strong>Porphyromonas gingivalis is a major human oral opportunistic pathogen and a key etiological agent of periodontal disease, contributing to inflammation and bone loss in the oral cavity. Periodontitis is not limited to oral health complications; it has also been associated with a range of systemic conditions, including coronary heart disease (CAD), respiratory disease, rheumatoid arthritis, chronic kidney disease (CKD), and certain types of cancer.</p><p><strong>Objectives: </strong>Immunization-based prevention of periodontitis appears to be a promising strategy; however, no vaccine is currently available for commercial use. In the present study, a novel vaccine candidate against P. gingivalis was proposed, consisting of a P. gingivalis protein, gingipain, glycosylated with the carbohydrate moiety of P. gingivalis lipopolysaccharide (LPS).</p><p><strong>Material and methods: </strong>Glycosylation of gingipain was achieved in Escherichia coli by introducing the Campylobacter jejuni N-glycosylation system, the P. gingivalis LPS biosynthetic pathway and the gingipain gene.</p><p><strong>Results: </strong>The neoglycoprotein was purified using column chromatography to a purity exceeding 99%, yielding a soluble antigen. The modified protein was recognized by commercial antibodies targeting the protein backbone, the carbohydrate moiety, and a custom monoclonal antibody specific to the purified LPS of P. gingivalis American Type Culture Collection (ATCC) 33277. The glycoprotein was used to immunize mice, and the resulting sera were analyzed for their ability to opsonize bacterial cells. The absence of detectable opsonization suggests that the elicited antibodies are more likely directed against the protein component of the vaccine rather than the glycan surface antigen.</p><p><strong>Conclusions: </strong>The final product was most likely assembled correctly, as it was recognized by LPS-specific antibodies. Further evaluation in an animal model of induced periodontitis is necessary to determine whether the elicited antibodies can effectively inhibit gingipain released by the pathogen. If this vaccine candidate demonstrates protective efficacy, the approach could accelerate and enhance the safety of vaccine design against a wide range of other pathogens.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically determined thyroid function and cerebral cortex structure: A Mendelian randomization study.","authors":"Xupeng Wu, Hong Liu, Liangliang Cui, Mengyan Mo, Changxin Li","doi":"10.17219/acem/199321","DOIUrl":"https://doi.org/10.17219/acem/199321","url":null,"abstract":"<p><strong>Background: </strong>A correlation between thyroid function and cognitive impairment has been well established; however, the impact of thyroid dysfunction on structural changes in the brain cortex remains largely unexplored.</p><p><strong>Objectives: </strong>The study describes a 2-sample Mendelian randomization (MR) analysis to elucidate the relationship between thyroid malfunction and brain structure and function.</p><p><strong>Material and methods: </strong>Eight phenotypes of thyroid function were extracted from THYROIDOMICS consortium by determining free thyroxine (FT4) and thyroid-stimulating hormone (TSH) levels in both men and women separately and together, as well as in individuals with increased or decreased TSH levels. The results were assessed in terms of overall brain cortical thickness and the surface area (SA) of grey matter, along with 34 specific measurements for various regions. The primary method employed for the analysis was the inverse-variance weighted (IVW) approach.</p><p><strong>Results: </strong>The data were subjected to MR Egger regression, Cochrane's Q statistic and leave-one-out analysis to determine the correlation between the variables. The FT4 in men, women and overall was statistically associated with cortical thickness of entorhinal cortex (EC). Overall TSH and TSH in men were associated with cortical thickness of caudal anterior cingulate. Additionally, in men, TSH levels showed an association with cortical thickness in the cuneus gyrus. Increased TSH was associated with decreased SA of lateral occipital (LO) and increased SA of lateral orbitofrontal, medial orbitofrontal and superior frontal cortex. Decreased TSH was negatively associated with the SA of pars opercularis (PO) and the cortical thickness of posterior cingulate cortex. No pleiotropy was detected.</p><p><strong>Conclusions: </strong>Our findings indicate a possible causal link between thyroid function and the cortical architecture of particular functional areas associated with neurodegenerative and psychiatric conditions.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between proinflammatory cytokines and pain intensity in patients with postherpetic neuralgia.","authors":"Jun Miao, Lu Wang, Min Feng","doi":"10.17219/acem/200267","DOIUrl":"https://doi.org/10.17219/acem/200267","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory response is involved in the pathogenesis of herpes zoster (HZ) and postherpetic neuralgia (PHN).</p><p><strong>Objectives: </strong>This study aimed to evaluate levels of proinflammatory factors at different stages of HZ and PHN.</p><p><strong>Material and methods: </strong>A total of 154 patients within 72 h of HZ onset and 30 healthy controls were included. Patients were followed up to 90 days. The levels of interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α) and C-reactive protein (CRP) were measured at baseline and 90 days. The visual analogue scale (VAS) was used to assess the intensity of pain and PHN patients were divided into mild-to-moderate pain and severe pain group.</p><p><strong>Results: </strong>Interleukin 6, TNF-α and CRP levels in HZ patients at baseline were significantly higher than in healthy controls and decreased as followed up to 90 days. Moreover, PHN patients had a higher level of IL-6, TNF-α or CRP at baseline and 90 days than non-PHN patients. In addition, PHN patients in the severe pain group had a notably higher baseline or 90-day IL-6, TNF-α and CRP level than in the mild-to-moderate pain group. However, the changes of IL-6, TNF-α and CRP levels between 90 days and baseline were significantly less pronounced in the severe pain group than in the mild-to-moderate pain group.</p><p><strong>Conclusions: </strong>The levels of proinflammatory cytokines were higher in HZ and PHN patients and associated with pain intensity in PNH patients. These findings suggest that repeated measurements of serum proinflammatory cytokines may aid in clinical management and guide anti-inflammatory treatment strategies.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parkin aggravates symptoms of preeclampsia through promoting mitophagy and apoptosis.","authors":"Li Wang, Xue Wang, Ying Zheng, Jiao Kong, Lin-Mei Zheng, Ai-Hua He, Xiao-Ju Chen","doi":"10.17219/acem/200059","DOIUrl":"https://doi.org/10.17219/acem/200059","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia is a serious pregnancy complication with significant maternal and fetal morbidity. Mitophagy plays a crucial role in its pathogenesis. The importance of this study lies in evaluating the role of parkin in preeclampsia, which may offer new insights into the management of this disease.</p><p><strong>Objectives: </strong>This study was designed to evaluate the role of parkin in preeclampsia.</p><p><strong>Material and methods: </strong>To induce a preeclampsia model, pregnant female rats were administered N-nitro-L-arginine methyl ester (L-NAME) subcutaneously at a dose of 50 mg/(kg·day) starting on gestational day 14 for 7 consecutive days. Uteroplacental tissues were then collected, and chorionic trophoblast cells were isolated. Systolic blood pressure (SBP) and urine protein content were measured on days 12 and 20 of pregnancy. Hematoxylin-eosin (H&E) staining and TUNEL staining were employed to assess pathological changes and apoptosis in uteroplacental tissues, respectively. Reverse transcription polymerase chain reaction (RT-qPCR) and western blot analysis were performed to evaluate mRNA and protein expression levels associated with cellular function, mitophagy and the PINK1/parkin signaling pathway.</p><p><strong>Results: </strong>Compared to the negavtive control (NC) group, rats in the model group showed elevated SBP and urine protein levels (p < 0.01). Chorionic trophoblast cells exhibited substantial damage, with significantly increased levels of apoptosis and autophagy. Moreover, parkin mRNA and protein expression levels were markedly upregulated in the model group. Overexpression of parkin in chorionic trophoblast cells enhanced apoptosis and mitophagy, while the autophagy inhibitor 3-methyladenine (3-MA) significantly alleviated the damage caused by overexpression of parkin.</p><p><strong>Conclusions: </strong>Parkin aggravates the symptoms of preeclampsia by increasing mitophagy and apoptosis.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of echinacoside on the regulation of mitochondrial fission induced by TBK1/Drp1 in rheumatoid arthritis.","authors":"Xiaoyan Wang, Zhufeng Chen, Shanshan Wu, Xuemei Fan","doi":"10.17219/acem/199920","DOIUrl":"https://doi.org/10.17219/acem/199920","url":null,"abstract":"<p><strong>Background: </strong>Dysregulated mitochondrial fission in synovial tissue is a key contributor to the progression of rheumatoid arthritis (RA), and echinacoside (ECH) has been shown to modulate this process in a mouse model of RA.</p><p><strong>Objectives: </strong>This study aimed to investigate the effects of echinacoside (ECH) on the proliferation and inflammatory response of human fibroblast-like synoviocytes (MH7A cells), and to elucidate the potential underlying mechanisms.</p><p><strong>Material and methods: </strong>The expression and co-localization of TANK-binding kinase 1 (TBK1) and phosphorylated dynamin-related protein 1 (p-Drp1) in synovial tissues from patients with and without RA were analyzed. MH7A cells were exposed to either ECH or 0.1% dimethyl sulfoxide (DMSO). Cell proliferation was detected using Cell Counting Kit-8 (CCK-8) assay and reactive oxygen species (ROS) expression was detected with dichlorofluorescin (DCFH) staining. The levels of interleukin (IL)-6, IL-8, tumor necrosis factor alpha (TNF-α), cyclooxygenase (COX)-2, IL-1β, TANK-binding kinase 1 (TBK1), and Drp1 and the oxidative stress markers NF-E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and NAD(P)H: quinone oxidoreductase 1 (NQO1) were measured using quantitative real-time polymerase chain reaction (qPCR). The mitochondrial morphology was detected with transmission electron microscopy (TEM), and the expression levels of p-TBK1 (S172), TBK1, p-Drp1 (S616), p-Drp1 (S637), and Drp1 were assessed using western blotting.</p><p><strong>Results: </strong>Compared to tissue from non-RA patients, RA synovial tissue exhibited higher expression and co-localization of TBK1 and phosphorylated Drp1 (p-Drp1). Following ECH treatment, MH7A cell proliferation and inflammatory cytokine secretion were reduced, while the expression of antioxidant stress markers was significantly increased. Furthermore, ECH treatment led to reduced levels of ROS, mitochondrial fragmentation and dysregulated mitochondrial fission in MH7A cells, along with decreased expression of p-TBK1 (Ser172) and p-Drp1 (Ser616), while p-Drp1 (Ser637) levels were increased.</p><p><strong>Conclusions: </strong>Echinacoside regulates abnormal mitochondrial fission via the TBK1/Drp1 pathway, reducing the proliferation and inflammatory response of MH7A cells.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined CB1 antagonist AM6545 and NOP agonist SCH221510 worsen DSS-induced colitis in mice.","authors":"Adam Fabisiak, Maria R Wołyniak, Fabiana Piscitelli, Roberta Verde, Vincenzo Di Marzo, Marta Zielińska, Weronika Machelak, Ewa Małecka-Wojciesko","doi":"10.17219/acem/203426","DOIUrl":"10.17219/acem/203426","url":null,"abstract":"<p><strong>Background: </strong>Despite the broad range of treatment options available for intestinal inflammation, the development of novel therapeutics remains essential due to the diminishing effectiveness of current therapies over time. Both the endocannabinoid system (ECS) and nociceptin/orphanin FQ peptide (NOP) receptors have been implicated in the pathogenesis of diseases associated with intestinal inflammation, highlighting their potential as therapeutic targets.</p><p><strong>Objectives: </strong>We hypothesized that an interaction exists between cannabinoid receptors 1 and 2 (CB1 and CB2) and the NOP receptor, which may hold therapeutic relevance for the treatment of colitis.</p><p><strong>Material and methods: </strong>In this study, we used 3 selective ligands: a CB1 antagonist (AM6545), a CB2 antagonist (AM630) and a NOP agonist (SCH221510) in a mouse model of colitis induced by 3% dextran sulfate sodium (DSS). Quantification of several secondary messengers was conducted using western blot analysis. Real-time quantitative polymerase chain reaction (qPCR) was employed to assess CB1 expression levels in colonic tissue, while liquid chromatography-mass spectrometry (LC-MS) was used to evaluate the concentrations of endocannabinoids and related lipid mediators.</p><p><strong>Results: </strong>We observed a statistically significant increase in the macroscopic score and a nonsignificant increase in the microscopic score in inflamed mice treated with both AM6545 and SCH221510 compared to those treated with SCH221510 alone. Additionally, the combination-treated group exhibited significantly lower levels of extracellular signal-regulated kinases 1/2 (ERK1/2) and significantly higher levels of phosphorylated protein kinase B (p-AKT) and β-arrestin relative to the SCH221510-only group.</p><p><strong>Conclusions: </strong>Our study offers novel insights into the interaction between the ECS and the NOP receptor, which may inform the development of new therapeutic strategies for inflammatory conditions such as colitis.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The translation into Polish, cultural adaptation, and initial validation of the Action Research Arm Test in subacute stroke patients.","authors":"Joanna Małecka, Magdalena Goliwąs, Katarzyna Adamczewska, Jacek Lewandowski, Dawid Łochyński","doi":"10.17219/acem/191775","DOIUrl":"10.17219/acem/191775","url":null,"abstract":"<p><strong>Background: </strong>In Poland, there are limited validated outcome measures to evaluate upper extremity function in stroke patients for clinical and research use. The Action Research Arm Test (ARAT) aims to assess functional performance of the upper extremities.</p><p><strong>Objectives: </strong>To translate and culturally adapt the original version of ARAT into Polish, and to determine its reliability and validity.</p><p><strong>Material and methods: </strong>A Polish version of ARAT (ARAT-PL) was developed using a forward-backward translation. The study then examined 60 patients with subacute stroke. Internal consistency (α), test-retest and inter-rater reliability (intra-class correlation (ICC), κ), standard error of measurement (SEM), minimal detectable change (MDC), and floor and ceiling effects were determined. The construct validity was evaluated using the method of hypothesis testing based on the results of correlations (rho) between subscale and total scores of the ARAT-PL and the upper and lower extremity section of the Fugl-Meyer Assessment (FMA-UE and FMA-LE).</p><p><strong>Results: </strong>The internal consistency of the total scores and subscale was excellent (α = 0.97-0.99). Test-retest and inter-rater reliability scores were almost perfect (κ = 0.85-1.0) and excellent for the total and subscale scores (ICC = 0.99-1). The SEM and MDC for the test-retest and inter-rater reliability were 0.479, 1.327 points and 0.335, 0.930 points, respectively. The ceiling effect amounted to 48%. The validity levels with respect to FMA-UE and FMA-LE were found to be high (rho ranging from 0.70 to 0.83) and moderate (rho ranging from 0.53 to 0.68), respectively.</p><p><strong>Conclusions: </strong>A Polish version of ARAT is a reliable and valid tool for assessing upper extremity function in subacute stroke patients in Poland. However, it appears to have a ceiling effect that limits differentiation of patients with mild upper limb impairment.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"1165-1173"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suicidal behavior and substance dependence.","authors":"Barbara Schneider, Lars Meiländer, Tilman Wetterling","doi":"10.17219/acem/207291","DOIUrl":"10.17219/acem/207291","url":null,"abstract":"<p><p>Suicidal behavior is a common psychiatric emergency and poses a significant challenge in mental health care. Substance use disorders are among the most frequently observed mental health conditions in individuals who die by suicide.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"1079-1084"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144705984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unfolding memories: Crafting digital life storybooks for dementia care via telehealth.","authors":"Ponnusamy Subramaniam, Preyaangka Thillainathan, Anthony Angwin, Shobha Sharma","doi":"10.17219/acem/194410","DOIUrl":"10.17219/acem/194410","url":null,"abstract":"<p><strong>Background: </strong>Dementia, a rapidly growing cognitive disorder, has a profound impact on the lives of individuals and their caregivers across the globe. Digital life storybooks have emerged as a promising non-pharmacological intervention to improve the wellbeing of people living with dementia (PLWD).</p><p><strong>Objectives: </strong>This study aims to investigate the feasibility of developing and applying a digital life storybook for PLWD using telehealth, while evaluating its impact on communication skills, quality of life (QoL) and satisfaction levels.</p><p><strong>Material and methods: </strong>A mixed-method study design will be employed, involving pairs of PLWD and their primary caregivers (dyads) recruited from a teaching hospital and a non-profit organization in Malaysia. The intervention involves the creation and use of a digital life storybook facilitated remotely via telehealth channels. Data will be collected at 6 points in time: prior to the commencement of development, prior to the submission of an application, on a biweekly basis, and at the conclusion of the assessment period. Quantitative measures will include the Holden Communication Scale, Quality of Life-Alzheimer's Disease Scale (QoL-AD) and Quality of the Caregiving Relationship (QCPR) questionnaire. Qualitative data will be gathered through validated open-ended questions.</p><p><strong>Results: </strong>Implications of the study include facilitating future research, contributing to person-centered care practices, and providing caregivers with tools to better understand and connect with PLWD. The findings will contribute to the understanding of the mechanisms through which digital life storybooks can benefit PLWD and their caregivers.</p><p><strong>Conclusion: </strong>The successful implementation of this protocol could have significant implications for dementia care in both formal and informal settings, and could ultimately improve the lives of those affected by dementia.</p>","PeriodicalId":7306,"journal":{"name":"Advances in Clinical and Experimental Medicine","volume":" ","pages":"1191-1200"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}