Extracellular vesicle最新文献_第7页

MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis 肾纤维化中巨噬细胞活化受EGFR米托贝诱导，其携带的源自小管上皮细胞的MHC-1B外泌体可抑制巨噬细胞活化

Extracellular vesicle Pub Date : 2023-01-01 DOI: 10.1016/j.vesic.2023.100024

Jin Guo , Xuanqi Liu , Haoming Song , Yong Gu , Jianying Niu , Lin Yang

引用次数: 0

Therapeutic and diagnostic potential of extracellular vesicles in amyotrophic lateral sclerosis 肌萎缩性侧索硬化症细胞外囊泡的治疗和诊断潜力

Extracellular vesicle Pub Date : 2023-01-01 DOI: 10.1016/j.vesic.2022.100019

Taylor J. Ellison , Steven L. Stice , Yao Yao

引用次数: 0

Multifunctional exosome-driven pancreatic cancer diagnostics and therapeutics 多功能外泌体驱动的胰腺癌诊断和治疗

Extracellular vesicle Pub Date : 2023-01-01 DOI: 10.1016/j.vesic.2023.100022

Sitong Zhang , Danyang Li , Yingjia Liu , Chunlian Qin , Lingjun Tong , Lizhou Xu

引用次数: 0

Extracellular vesicles as novel therapeutic targets and diagnosis markers 细胞外囊泡作为新的治疗靶点和诊断标志物

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100017

Yu Zhang , Weiliang Wu , Xiangbin Pan , Yanli Wang , Chengjie Wu , Lin Lu , Xi-Yong Yu , Yangxin Li

引用次数: 1

The interactions between extracellular vesicles and mesenchymal stem cells: Their potential roles in osteoarthritis development and cartilage repair 细胞外小泡和间充质干细胞之间的相互作用：它们在骨关节炎发展和软骨修复中的潜在作用

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100011

Qiong Yang , Danyang Yue , Qian Ren , Guoqing Xia , Baihui Zhang , Yinyin Qin , Tianfei Ran , Min Wang , Li Pei , Jun Pan

{"title":"The interactions between extracellular vesicles and mesenchymal stem cells: Their potential roles in osteoarthritis development and cartilage repair","authors":"Qiong Yang , Danyang Yue , Qian Ren , Guoqing Xia , Baihui Zhang , Yinyin Qin , Tianfei Ran , Min Wang , Li Pei , Jun Pan","doi":"10.1016/j.vesic.2022.100011","DOIUrl":"10.1016/j.vesic.2022.100011","url":null,"abstract":"<div><p>Osteoarthritis (OA) is a chronic cartilage degeneration disease affecting total joint and with a wide global impact. Stem cell therapy has emerged as a potential stock for the treatment of OA. Exosomes are the mainstay of the paracrine function in stem cells, acting as an important mediator of intercellular communication. The purpose of this review is to sort out the close relationships among OA, MSCs, and exosomes. We find though majority of researches on exosomes focus on normal MSC-derived exosomes to restore tissues, studies demonstrate that OA or inflammation-induced microenvironment can alter exosomes, which in turn affect the biological behaviors and functions of receptor cells in the joint cavity, including proliferation and differentiation of MSCs. Exosomes, MSCs and OA form a triangular relationship. More future research directions are exploring the effects of OA-derived exosomes on chondrogenic differentiation of MSCs, especially on mitochondria to disclose the reasons for failure endogenous repair by MSCs, and normalizing the OA exosomes to treat OA.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100011"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041722000063/pdfft?md5=8eb8bca79abdb7597470a39e2db3d70e&pid=1-s2.0-S2773041722000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45112342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Extracellular vesicle therapy for non-ischemic heart failure: A systematic review of preclinical studies 非缺血性心力衰竭的细胞外囊泡治疗:临床前研究的系统综述

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100009

Ramana Vaka , Sophie Van Remortel , Valentina Ly , Darryl R. Davis

{"title":"Extracellular vesicle therapy for non-ischemic heart failure: A systematic review of preclinical studies","authors":"Ramana Vaka , Sophie Van Remortel , Valentina Ly , Darryl R. Davis","doi":"10.1016/j.vesic.2022.100009","DOIUrl":"10.1016/j.vesic.2022.100009","url":null,"abstract":"<div><p>Cell-derived extracellular vesicles (EVs) are being actively explored as a novel acellular approach to heart failure. Accumulating evidence suggests EVs improve cardiac function in non-ischemic heart failure, but the strength and consistency of this effect is unknown. As such, we critically appraised the preclinical literature supporting the ability of EVs to improve cardiac function in animal models of non-ischemic heart failure. After a systematic search that yielded 18 studies, we determined the overall effect of EV treatment on left ventricular function and performed regression analysis to identify EV effects on clinically relevant parameters. EV treatment uniformly improved ejection fraction (2.41 [95% CI: 1.76, 3.06; p < 0.00001; I<sup>2</sup> = 46%]) and fractional shortening (2.22 [95% CI: 1.40, 3.05; p < 0.00001; I<sup>2</sup> = 56%]) as compared to untreated control animals. Secondary outcomes (such as left ventricular volumes or myocardial fibrosis) were similarly improved in EV treated animals. Although the number of studies included in the analysis was modest, there was no evidence for a publication bias. In conclusion, EV treatment improves cardiac function in preclinical models of non-ischemic heart failure. Although EVs originated from dissimilar cell lines and were applied to a variety of different animal models, we observed a consistent improvement in cardiac function suggesting application of EVs to non-ischemic heart failure has merit and warrants future attention.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100009"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277304172200004X/pdfft?md5=de9270248b05cc7b64de99fdf0488d78&pid=1-s2.0-S277304172200004X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43654404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Engineered exosomes targeting MYC reverse the proneural-mesenchymal transition and extend survival of glioblastoma 靶向MYC的工程外泌体逆转前膜-间充质转化并延长胶质母细胞瘤的生存期

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100014

Amanda R. Haltom , Wafa E. Hassen , Janine Hensel , Jiha Kim , Hikaru Sugimoto , Bingrui Li , Kathleen M. McAndrews , Meagan R. Conner , Michelle L. Kirtley , Xin Luo , Bingqing Xie , Olga V. Volpert , Susan Olalekan , Natalia Maltsev , Anindita Basu , Valerie S. LeBleu , Raghu Kalluri

{"title":"Engineered exosomes targeting MYC reverse the proneural-mesenchymal transition and extend survival of glioblastoma","authors":"Amanda R. Haltom , Wafa E. Hassen , Janine Hensel , Jiha Kim , Hikaru Sugimoto , Bingrui Li , Kathleen M. McAndrews , Meagan R. Conner , Michelle L. Kirtley , Xin Luo , Bingqing Xie , Olga V. Volpert , Susan Olalekan , Natalia Maltsev , Anindita Basu , Valerie S. LeBleu , Raghu Kalluri","doi":"10.1016/j.vesic.2022.100014","DOIUrl":"10.1016/j.vesic.2022.100014","url":null,"abstract":"<div><p>Dysregulated Myc signaling is a key oncogenic pathway in glioblastoma multiforme (GBM). Yet, effective therapeutic targeting of Myc continues to be challenging. Here, we demonstrate that exosomes generated from human bone marrow mesenchymal stem cells (MSCs) engineered to encapsulate siRNAs targeting Myc (iExo-Myc) localize to orthotopic GBM tumors in mice. Treatment of late stage GBM tumors with iExo-Myc inhibits proliferation and angiogenesis, suppresses tumor growth, and extends survival. Transcriptional profiling of tumors reveals that the mesenchymal transition and estrogen receptor signaling pathways are impacted by Myc inhibition. Single nuclei RNA sequencing (snRNA-seq) shows that iExo-Myc treatment induces transcriptional repression of multiple growth factor and interleukin signaling pathways, triggering a mesenchymal to proneural transition and shifting the cellular landscape of the tumor. These data confirm that Myc is an effective anti-glioma target and that iExo-Myc offers a feasible, readily translational strategy to inhibit challenging oncogene targets for the treatment of brain tumors.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100014"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373511/pdf/nihms-1913722.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9893951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Inhalable exosomes outperform liposomes as mRNA and protein drug carriers to the lung 可吸入外泌体作为肺mRNA和蛋白质药物载体优于脂质体

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100002

Kristen D. Popowski , Blanca López de Juan Abad , Arianna George , Dylan Silkstone , Elizabeth Belcher , Jaewook Chung , Asma Ghodsi , Halle Lutz , Jada Davenport , Mallory Flanagan , Jorge Piedrahita , Phuong-Uyen C. Dinh , Ke Cheng

{"title":"Inhalable exosomes outperform liposomes as mRNA and protein drug carriers to the lung","authors":"Kristen D. Popowski , Blanca López de Juan Abad , Arianna George , Dylan Silkstone , Elizabeth Belcher , Jaewook Chung , Asma Ghodsi , Halle Lutz , Jada Davenport , Mallory Flanagan , Jorge Piedrahita , Phuong-Uyen C. Dinh , Ke Cheng","doi":"10.1016/j.vesic.2022.100002","DOIUrl":"10.1016/j.vesic.2022.100002","url":null,"abstract":"<div><p>Respiratory diseases are among the leading causes of morbidity and mortality worldwide, coupled with the ongoing coronavirus disease 2019 (COVID-19) pandemic. mRNA lipid nanoparticle (LNP) vaccines have been developed, but their intramuscular delivery limits pulmonary bioavailability. Inhalation of nanoparticle therapeutics offers localized drug delivery that minimizes off targeted adverse effects and has greater patient compliance. However, LNP platforms require extensive reformulation for inhaled delivery. Lung-derived extracellular vesicles (Lung-Exo) offer a biological nanoparticle alternative that is naturally optimized for mRNA translation and delivery to pulmonary cells. We compared the biodistribution of Lung-Exo against commercially standard biological extracellular vesicles (HEK-Exo) and LNPs (Lipo), where Lung-Exo exhibited superior mRNA and protein cargo distribution to and retention in the bronchioles and parenchyma following nebulization administration. This suggests that inhaled Lung-Exo can deliver mRNA and protein drugs with enhanced pulmonary bioavailability and therapeutic efficacy.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100002"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10287060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles 通过膜挤压法制备的细胞源性纳米囊泡是天然细胞外囊泡的良好替代品

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100004

Yi Wen , Qin Fu , Ashley Soliwoda , Sheng Zhang , Mingfeng Zheng , Wenjun Mao , Yuan Wan

{"title":"Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles","authors":"Yi Wen , Qin Fu , Ashley Soliwoda , Sheng Zhang , Mingfeng Zheng , Wenjun Mao , Yuan Wan","doi":"10.1016/j.vesic.2022.100004","DOIUrl":"10.1016/j.vesic.2022.100004","url":null,"abstract":"<div><p>Extracellular vesicles (EV) as drug delivery nanocarriers are under intense investigation. Although clinical-grade EVs have been produced on a large-scale, low yield and high production costs of natural EVs (nEV) limit the relevant industrial translation. Recent studies show that mechanical extrusion of cells can generate nEV-like cell-derived nanovesicles (CNV) which can also be used as drug nanocarriers. Moreover, in comparison with nEVs, CNVs have similar physicochemical properties. Nevertheless, a comprehensive comparison of cargo between nEVs and CNVs has not been investigated yet. Therefore, the aim of this study is to profile and compare CNVs to nEVs. Our results show that no significant difference was found in size, morphology, and classical markers between nEVs and CNVs derived from MDA-MB-231 cells. Protein sequencing data reveals the similarity of membrane proteins between the two groups was <span><math><mo>∼</mo></math></span>71%, while it was <span><math><mo>∼</mo></math></span>21% when pertaining to total protein cargo. Notably, a high similarity of membrane proteins was also found between nEVs and CNVs derived from eight additional cancer cell lines. Moreover, analysis of the top 1000 small RNAs with RNA sequencing showed a <span><math><mo>∼</mo></math></span>65% similarity between the two groups. Altogether, we infer from the high similarity of membrane proteins and small RNA cargo that CNVs can be a good substitute for nEVs. In brief, our findings support previous studies with a notion that CNVs yield comparable performance with nEVs and could pave the way for clinical implementation of CNV-based therapeutics in the future.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100004"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/77/95/nihms-1859360.PMC9794200.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10509824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Sucrose-based cryoprotective storage of extracellular vesicles 以蔗糖为基础的细胞外囊泡冷冻保护储存

Extracellular vesicle Pub Date : 2022-12-01 DOI: 10.1016/j.vesic.2022.100016

Sierra A. Walker , Irina Davidovich , Yubo Yang , Andrew Lai , Jenifer Pendiuk Goncalves , Vatsal Deliwala , Sara Busatto , Shane Shapiro , Na’ama Koifman , Carlos Salomon , Yeshayahu Talmon , Joy Wolfram

{"title":"Sucrose-based cryoprotective storage of extracellular vesicles","authors":"Sierra A. Walker , Irina Davidovich , Yubo Yang , Andrew Lai , Jenifer Pendiuk Goncalves , Vatsal Deliwala , Sara Busatto , Shane Shapiro , Na’ama Koifman , Carlos Salomon , Yeshayahu Talmon , Joy Wolfram","doi":"10.1016/j.vesic.2022.100016","DOIUrl":"10.1016/j.vesic.2022.100016","url":null,"abstract":"<div><p>Advancements in extracellular vesicle (EV) studies necessitate the development of optimized storage conditions to ensure preservation of physical and biochemical characteristics. In this study, the most common buffer for EV storage (phosphate-buffered saline/PBS) was compared to a cryoprotective 5% sucrose solution. The size distribution and concentration of EVs from two different sources changed to a greater extent after −80 °C storage in PBS compared to the sucrose solution. Additionally, molecular surface protrusions and transmembrane proteins were more prevalent in EVs stored in the sucrose solution compared to those stored in PBS. This study demonstrates, for the first time, that distinct ring-like molecular complexes and cristae-like folded membranous structures are visible upon EV degradation. Taken together, the size, concentration, molecular surface extensions, and transmembrane proteins of EVs varied substantially based on the buffer used for −80 °C storage, suggesting that biocompatible cryoprotectants, such as sucrose, should be considered for EV studies.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100016"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041722000117/pdfft?md5=86b94a603c3bfe47ab1d92a96499da88&pid=1-s2.0-S2773041722000117-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42655161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7