Kai Qiao , Xinyue Cui , Jiamin Gao , Fengyi Yu , Haohao Liu , Yichen Dai , Jiming Liu , Yu Yang , Xunde Xian , Jinming Hu , Junnan Tang , Xiaolin Cui
{"title":"Roles of extracellular vesicles derived from immune cells in atherosclerosis","authors":"Kai Qiao , Xinyue Cui , Jiamin Gao , Fengyi Yu , Haohao Liu , Yichen Dai , Jiming Liu , Yu Yang , Xunde Xian , Jinming Hu , Junnan Tang , Xiaolin Cui","doi":"10.1016/j.vesic.2023.100028","DOIUrl":"https://doi.org/10.1016/j.vesic.2023.100028","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49857393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Therapeutic and diagnostic potential of extracellular vesicles in amyotrophic lateral sclerosis","authors":"Taylor J. Ellison , Steven L. Stice , Yao Yao","doi":"10.1016/j.vesic.2022.100019","DOIUrl":"https://doi.org/10.1016/j.vesic.2022.100019","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49857399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu Zhang , Weiliang Wu , Xiangbin Pan , Yanli Wang , Chengjie Wu , Lin Lu , Xi-Yong Yu , Yangxin Li
{"title":"Extracellular vesicles as novel therapeutic targets and diagnosis markers","authors":"Yu Zhang , Weiliang Wu , Xiangbin Pan , Yanli Wang , Chengjie Wu , Lin Lu , Xi-Yong Yu , Yangxin Li","doi":"10.1016/j.vesic.2022.100017","DOIUrl":"10.1016/j.vesic.2022.100017","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are membranous vesicles secreted from cells to the outside and are widely present in body fluids. The most commonly used method to isolate EVs is ultracentrifugation. EVs contain lipids, proteins, genetic material, and organelles, which can be used as communication carriers between different cells and potential disease biomarkers. EVs secreted by different cells (cancer cells, adipocytes, mesenchymal stem cells, immune cells et al.) play different functions. With the development of technology, EVs can also be engineered to deliver drugs. EVs also play an important role in the progression, prevention, diagnosis, and treatment of Corona Virus Disease 2019 (COVID-19).</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100017"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041722000129/pdfft?md5=e27d3ccfc78e3fb048ad92d36880420a&pid=1-s2.0-S2773041722000129-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44174769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qiong Yang , Danyang Yue , Qian Ren , Guoqing Xia , Baihui Zhang , Yinyin Qin , Tianfei Ran , Min Wang , Li Pei , Jun Pan
{"title":"The interactions between extracellular vesicles and mesenchymal stem cells: Their potential roles in osteoarthritis development and cartilage repair","authors":"Qiong Yang , Danyang Yue , Qian Ren , Guoqing Xia , Baihui Zhang , Yinyin Qin , Tianfei Ran , Min Wang , Li Pei , Jun Pan","doi":"10.1016/j.vesic.2022.100011","DOIUrl":"10.1016/j.vesic.2022.100011","url":null,"abstract":"<div><p>Osteoarthritis (OA) is a chronic cartilage degeneration disease affecting total joint and with a wide global impact. Stem cell therapy has emerged as a potential stock for the treatment of OA. Exosomes are the mainstay of the paracrine function in stem cells, acting as an important mediator of intercellular communication. The purpose of this review is to sort out the close relationships among OA, MSCs, and exosomes. We find though majority of researches on exosomes focus on normal MSC-derived exosomes to restore tissues, studies demonstrate that OA or inflammation-induced microenvironment can alter exosomes, which in turn affect the biological behaviors and functions of receptor cells in the joint cavity, including proliferation and differentiation of MSCs. Exosomes, MSCs and OA form a triangular relationship. More future research directions are exploring the effects of OA-derived exosomes on chondrogenic differentiation of MSCs, especially on mitochondria to disclose the reasons for failure endogenous repair by MSCs, and normalizing the OA exosomes to treat OA.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100011"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041722000063/pdfft?md5=8eb8bca79abdb7597470a39e2db3d70e&pid=1-s2.0-S2773041722000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45112342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramana Vaka , Sophie Van Remortel , Valentina Ly , Darryl R. Davis
{"title":"Extracellular vesicle therapy for non-ischemic heart failure: A systematic review of preclinical studies","authors":"Ramana Vaka , Sophie Van Remortel , Valentina Ly , Darryl R. Davis","doi":"10.1016/j.vesic.2022.100009","DOIUrl":"10.1016/j.vesic.2022.100009","url":null,"abstract":"<div><p>Cell-derived extracellular vesicles (EVs) are being actively explored as a novel acellular approach to heart failure. Accumulating evidence suggests EVs improve cardiac function in non-ischemic heart failure, but the strength and consistency of this effect is unknown. As such, we critically appraised the preclinical literature supporting the ability of EVs to improve cardiac function in animal models of non-ischemic heart failure. After a systematic search that yielded 18 studies, we determined the overall effect of EV treatment on left ventricular function and performed regression analysis to identify EV effects on clinically relevant parameters. EV treatment uniformly improved ejection fraction (2.41 [95% CI: 1.76, 3.06; p < 0.00001; I<sup>2</sup> = 46%]) and fractional shortening (2.22 [95% CI: 1.40, 3.05; p < 0.00001; I<sup>2</sup> = 56%]) as compared to untreated control animals. Secondary outcomes (such as left ventricular volumes or myocardial fibrosis) were similarly improved in EV treated animals. Although the number of studies included in the analysis was modest, there was no evidence for a publication bias. In conclusion, EV treatment improves cardiac function in preclinical models of non-ischemic heart failure. Although EVs originated from dissimilar cell lines and were applied to a variety of different animal models, we observed a consistent improvement in cardiac function suggesting application of EVs to non-ischemic heart failure has merit and warrants future attention.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100009"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277304172200004X/pdfft?md5=de9270248b05cc7b64de99fdf0488d78&pid=1-s2.0-S277304172200004X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43654404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amanda R. Haltom , Wafa E. Hassen , Janine Hensel , Jiha Kim , Hikaru Sugimoto , Bingrui Li , Kathleen M. McAndrews , Meagan R. Conner , Michelle L. Kirtley , Xin Luo , Bingqing Xie , Olga V. Volpert , Susan Olalekan , Natalia Maltsev , Anindita Basu , Valerie S. LeBleu , Raghu Kalluri
{"title":"Engineered exosomes targeting MYC reverse the proneural-mesenchymal transition and extend survival of glioblastoma","authors":"Amanda R. Haltom , Wafa E. Hassen , Janine Hensel , Jiha Kim , Hikaru Sugimoto , Bingrui Li , Kathleen M. McAndrews , Meagan R. Conner , Michelle L. Kirtley , Xin Luo , Bingqing Xie , Olga V. Volpert , Susan Olalekan , Natalia Maltsev , Anindita Basu , Valerie S. LeBleu , Raghu Kalluri","doi":"10.1016/j.vesic.2022.100014","DOIUrl":"10.1016/j.vesic.2022.100014","url":null,"abstract":"<div><p>Dysregulated Myc signaling is a key oncogenic pathway in glioblastoma multiforme (GBM). Yet, effective therapeutic targeting of Myc continues to be challenging. Here, we demonstrate that exosomes generated from human bone marrow mesenchymal stem cells (MSCs) engineered to encapsulate siRNAs targeting Myc (iExo-Myc) localize to orthotopic GBM tumors in mice. Treatment of late stage GBM tumors with iExo-Myc inhibits proliferation and angiogenesis, suppresses tumor growth, and extends survival. Transcriptional profiling of tumors reveals that the mesenchymal transition and estrogen receptor signaling pathways are impacted by Myc inhibition. Single nuclei RNA sequencing (snRNA-seq) shows that iExo-Myc treatment induces transcriptional repression of multiple growth factor and interleukin signaling pathways, triggering a mesenchymal to proneural transition and shifting the cellular landscape of the tumor. These data confirm that Myc is an effective anti-glioma target and that iExo-Myc offers a feasible, readily translational strategy to inhibit challenging oncogene targets for the treatment of brain tumors.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100014"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10373511/pdf/nihms-1913722.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9893951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kristen D. Popowski , Blanca López de Juan Abad , Arianna George , Dylan Silkstone , Elizabeth Belcher , Jaewook Chung , Asma Ghodsi , Halle Lutz , Jada Davenport , Mallory Flanagan , Jorge Piedrahita , Phuong-Uyen C. Dinh , Ke Cheng
{"title":"Inhalable exosomes outperform liposomes as mRNA and protein drug carriers to the lung","authors":"Kristen D. Popowski , Blanca López de Juan Abad , Arianna George , Dylan Silkstone , Elizabeth Belcher , Jaewook Chung , Asma Ghodsi , Halle Lutz , Jada Davenport , Mallory Flanagan , Jorge Piedrahita , Phuong-Uyen C. Dinh , Ke Cheng","doi":"10.1016/j.vesic.2022.100002","DOIUrl":"10.1016/j.vesic.2022.100002","url":null,"abstract":"<div><p>Respiratory diseases are among the leading causes of morbidity and mortality worldwide, coupled with the ongoing coronavirus disease 2019 (COVID-19) pandemic. mRNA lipid nanoparticle (LNP) vaccines have been developed, but their intramuscular delivery limits pulmonary bioavailability. Inhalation of nanoparticle therapeutics offers localized drug delivery that minimizes off targeted adverse effects and has greater patient compliance. However, LNP platforms require extensive reformulation for inhaled delivery. Lung-derived extracellular vesicles (Lung-Exo) offer a biological nanoparticle alternative that is naturally optimized for mRNA translation and delivery to pulmonary cells. We compared the biodistribution of Lung-Exo against commercially standard biological extracellular vesicles (HEK-Exo) and LNPs (Lipo), where Lung-Exo exhibited superior mRNA and protein cargo distribution to and retention in the bronchioles and parenchyma following nebulization administration. This suggests that inhaled Lung-Exo can deliver mRNA and protein drugs with enhanced pulmonary bioavailability and therapeutic efficacy.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"1 ","pages":"Article 100002"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10287060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}