Extracellular vesicle最新文献_第8页

Exploring emerging concepts of exosomes for the diagnosis, prognosis, and therapeutics of brain cancers 探索外泌体用于脑癌诊断、预后和治疗的新概念

Extracellular vesicle Pub Date : 2024-06-01 Epub Date: 2024-04-20 DOI: 10.1016/j.vesic.2024.100038

Subhrajyoti Banerjee, Vriti Sharma, Chitrangada Das Mukhopadhyay

{"title":"Exploring emerging concepts of exosomes for the diagnosis, prognosis, and therapeutics of brain cancers","authors":"Subhrajyoti Banerjee, Vriti Sharma, Chitrangada Das Mukhopadhyay","doi":"10.1016/j.vesic.2024.100038","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100038","url":null,"abstract":"<div><p>Brain cancers, a global menace with high mortality rates, remain a profound challenge, particularly in treating the most lethal type of malignant tumors like glioblastoma multiforme (GBM). The crucial need for prompt identification and precise prognosis drives the exploration of exosomes as non-invasive tools. Exosomes are generated by various cells, especially malignant cells, facilitating intercellular communication in the tumor microenvironment, fostering glioma progression by conveying molecular modifiers, and offering molecular insights. These attributes hold promise for enhancing glioma diagnosis and prognosis. These nanosized messengers influence cancer dynamics, aiding tailored treatment strategies for improved patient outcomes. This article outlines the significance of exosomes in glioma progression, highlighting recent advancements in diagnostic and therapeutic strategies utilizing bioactive molecules as biomarkers like miRNAs, circ-RNAs, genes, and proteins in a variety of brain tumors, supported by advanced isolation and identification techniques. The relevance of circulating exosomes to various brain malignancies particularly glioblastoma, astrocytoma, craniopharyngiomas, meningioma, and more, has been discussed. It elucidates tumor immune cross-talk and underscores diagnostic-worthy exosomal biomarkers like miRNAs, circ-RNAs, genes, and proteins. Additionally, it delineates promising avenues for forthcoming investigations.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"3 ","pages":"Article 100038"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000052/pdfft?md5=800612686c103af535500b037ca6ecd0&pid=1-s2.0-S2773041724000052-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140621949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding molecular characteristics of extracellular vesicles derived from different types of mesenchymal stem cells for therapeutic translation 了解源自不同类型间充质干细胞的细胞外囊泡的分子特征，促进治疗转化

Extracellular vesicle Pub Date : 2024-06-01 Epub Date: 2024-03-02 DOI: 10.1016/j.vesic.2024.100034

Zuo Ding , Zachary F. Greenberg , Maria Fernanda Serafim , Samantha Ali , Julia C. Jamieson , Dmitry O. Traktuev , Keith March , Mei He

{"title":"Understanding molecular characteristics of extracellular vesicles derived from different types of mesenchymal stem cells for therapeutic translation","authors":"Zuo Ding , Zachary F. Greenberg , Maria Fernanda Serafim , Samantha Ali , Julia C. Jamieson , Dmitry O. Traktuev , Keith March , Mei He","doi":"10.1016/j.vesic.2024.100034","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100034","url":null,"abstract":"<div><p>Mesenchymal stem cells (MSCs) have been studied for decades as candidates for cellular therapy, and their secretome, including secreted extracellular vesicles (EVs), has been identified to contribute significantly to regenerative and reparative functions. Emerging evidence has suggested that MSC-EVs alone, could be used as therapeutics that emulate the biological function of MSCs. However, just as with MSCs, MSC-EVs have been shown to vary in composition, depending on the tissue source of the MSCs as well as the protocols employed in culturing the MSCs and obtaining the EVs. Therefore, the importance of careful choice of cell sources and culture environments is receiving increasing attention. Many factors contribute to the therapeutic potential of MSC-EVs, including the source tissue, isolation technique, and culturing conditions. This review illustrates the molecular landscape of EVs derived from different types of MSC cells along with culture strategies. A thorough analysis of publicly available omic datasets was performed to advance the precision understanding of MSC-EVs with unique tissue source-dependent molecular characteristics. The tissue-specific protein and miRNA-driven Reactome ontology analysis was used to reveal distinct patterns of top Reactome ontology pathways across adipose, bone marrow, and umbilical MSC-EVs. Moreover, a meta-analysis assisted by an AI technique was used to analyze the published literature, providing insights into the therapeutic translation of MSC-EVs based on their source tissues.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"3 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000015/pdfft?md5=fd73befeeaf3fda864de7baf5d943dab&pid=1-s2.0-S2773041724000015-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140016031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the horizon of EV-RNAs: LncRNAs in EVs as biomarkers for disease pathways 扩大ev - rna的视野:ev中的lncrna作为疾病通路的生物标志物

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-05-20 DOI: 10.1016/j.vesic.2023.100025

Michail Spanos , Priyanka Gokulnath , Emeli Chatterjee , Guoping Li , Dimitrios Varrias , Saumya Das

{"title":"Expanding the horizon of EV-RNAs: LncRNAs in EVs as biomarkers for disease pathways","authors":"Michail Spanos , Priyanka Gokulnath , Emeli Chatterjee , Guoping Li , Dimitrios Varrias , Saumya Das","doi":"10.1016/j.vesic.2023.100025","DOIUrl":"https://doi.org/10.1016/j.vesic.2023.100025","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are membrane-bound nanoparticles with different types of cargo released by cells and postulated to mediate functions such as intercellular communications. Recent studies have shown that long non-coding RNAs (lncRNAs) or their fragments are present as cargo within EVs. LncRNAs are a heterogeneous group of RNA species with a length exceeding 200 nucleotides with diverse functions in cells based on their localization. While lncRNAs are known for their important functions in cellular regulation, their presence and role in EVs have only recently been explored. While certain studies have observed EV-lncRNAs to be tissue- and disease-specific, it remains to be determined whether or not this is a global observation. Nonetheless, these molecules have demonstrated promising potential to serve as new diagnostic and prognostic biomarkers. In this review, we critically evaluate the role of EV-derived lncRNAs in several prevalent diseases, including cancer, cardiovascular diseases, and neurodegenerative diseases, with a specific focus on their role as biomarkers.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"2 ","pages":"Article 100025"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49857394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Current challenges surrounding exosome treatments. 当前围绕外泌体治疗的挑战。

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-04-29 DOI: 10.1016/j.vesic.2023.100023

Eileen Tzng, Nathan Bayardo, Phillip C Yang

引用次数: 0

Current challenges surrounding exosome treatments 当前围绕外泌体治疗的挑战

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-04-29 DOI: 10.1016/j.vesic.2023.100023

Eileen Tzng, Nathan Bayardo, Phillip C. Yang

引用次数: 0

Guidelines for clinical translation and commercialization of extracellular vesicles and exosomes based therapeutics 基于细胞外囊泡和外泌体的治疗方法的临床翻译和商业化指南

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-09-30 DOI: 10.1016/j.vesic.2023.100029

Ke Cheng , Raghu Kalluri

引用次数: 0

Roles of extracellular vesicles derived from immune cells in atherosclerosis 免疫细胞来源的细胞外囊泡在动脉粥样硬化中的作用

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-07-24 DOI: 10.1016/j.vesic.2023.100028

Kai Qiao , Xinyue Cui , Jiamin Gao , Fengyi Yu , Haohao Liu , Yichen Dai , Jiming Liu , Yu Yang , Xunde Xian , Jinming Hu , Junnan Tang , Xiaolin Cui

引用次数: 0

Engineered IL-15/IL-15Rα-expressing cellular vesicles promote T cell anti-tumor immunity 工程化表达IL-15/ il - 15r α的细胞囊泡促进T细胞抗肿瘤免疫

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2022-12-27 DOI: 10.1016/j.vesic.2022.100021

Wenli Fang , Liyan Li , Zhongda Lin , Yuli Zhang , Zhangyan Jing , Yuan Li , Zhirang Zhang , Linlin Hou , Xin Liang , Xingding Zhang , Xudong Zhang

引用次数: 5

MHC-1B carried exosomes derived from tubular epithelial cell induced by the EGFR mimotope inhibit macrophage activation in renal fibrosis 肾纤维化中巨噬细胞活化受EGFR米托贝诱导，其携带的源自小管上皮细胞的MHC-1B外泌体可抑制巨噬细胞活化

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-04-03 DOI: 10.1016/j.vesic.2023.100024

Jin Guo , Xuanqi Liu , Haoming Song , Yong Gu , Jianying Niu , Lin Yang

引用次数: 0

CD63-snorkel tagging for isolation of exosomes CD63-snorkel标记法分离外泌体

Extracellular vesicle Pub Date : 2023-12-01 Epub Date: 2023-10-26 DOI: 10.1016/j.vesic.2023.100031

Chaoshan Han , Junjie Yang , Tingting Yin , Junqing An , Aijun Qiao , Yangpo Cao , Yuliang Feng , Haocheng Lu , Ying Wang , Liang Yang , Gangjian Qin

{"title":"CD63-snorkel tagging for isolation of exosomes","authors":"Chaoshan Han , Junjie Yang , Tingting Yin , Junqing An , Aijun Qiao , Yangpo Cao , Yuliang Feng , Haocheng Lu , Ying Wang , Liang Yang , Gangjian Qin","doi":"10.1016/j.vesic.2023.100031","DOIUrl":"https://doi.org/10.1016/j.vesic.2023.100031","url":null,"abstract":"<div><p>Exosomes (Exo) are important mediators of inter-cellular communications; however, no effective method is available for isolating, thus characterizing, cellular-specific exosomes <em>in vivo</em>. Since CD63 is a reliable marker for exosomes, we have developed a tagging strategy, term “CD63-Snorkel (CD63-SNKL)”, in which CD63 at its intracellular C-terminus was fused to a fragment of PDGFRB that contains the transmembrane domain tethered to multiple epitope tags (HA, His, and FLAG) displayed in tandem on surface. We found that the CD63-SNKL protein has similar subcellular localizations as endogenous CD63 and can be effectively sorted into Exo. Furthermore, Exo secreted from CD63-SNKL–transduced cells can be effectively captured on anti-HA magnetic beads and eluted with HA peptides. Thus, CD63-SNKL may be engineered for isolating and tracking endogenous tissue-specific Exo <em>in vivo</em>.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"2 ","pages":"Article 100031"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041723000100/pdfft?md5=4118b6e1865381af9c561c620692b519&pid=1-s2.0-S2773041723000100-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92099123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0