Extracellular vesicle最新文献

{"title":"Encapsulating extracellular vesicles with a minimal RISC complex as novel gene silencing tool","authors":"Tao Qiu,&nbsp;Yu Yan,&nbsp;Rui Hu,&nbsp;Yuan Yi,&nbsp;Guowu Liu,&nbsp;Wenqiang Lu,&nbsp;Xin Zhou,&nbsp;Ke Xu","doi":"10.1016/j.vesic.2025.100094","DOIUrl":"10.1016/j.vesic.2025.100094","url":null,"abstract":"<div><div>Gene silencing modalities including small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs) have prospered in both fundamental research and clinical translations in recent years, with delivery platform being one of the key elements for success. Extracellular vesicles (EVs) as natural carriers for cell-cell communication have been engineered in a variety of ways as delivery platform for gene silencing, yet facing limited efficiency and reproducibility. In this study, we developed a new strategy for engineering EVs as gene silencing tool. A minimal RNA-induced silencing complex (RISC), composing of modified Argonaute 2 (AGO2) protein and specially designed guide strand RNAs, were encapsulated into EVs and elicited prominent EGFP silencing in proof-of-concept study. This modular EVs platform, which we named as minRISC-EVs, efficiently silenced iNOS expression in M1 macrophages as well as STAT6/A20 expression in M2 macrophages, enabling macrophages polarization towards desired directions. The macrophage modulating ability was further validated <em>in vivo</em>, as minRISC-EVs against iNOS alleviated mice lung inflammation in lipopolysaccharide (LPS)-induced acute lung injury model, and minRISC-EVs against STAT6/A20 inhibited B16F10 tumor progression in the tumor xenograft model. In summary, minRISC-EVs can be utilized as novel gene silencing tool, and hold great promise for clinical translation in the future.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100094"},"PeriodicalIF":0.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and characterization of platelet-derived extracellular vesicle analogues from lyophilized platelets","authors":"Xin Xin,&nbsp;Ingrid Dijkgraaf,&nbsp;Tilman M. Hackeng,&nbsp;Rory R. Koenen","doi":"10.1016/j.vesic.2025.100095","DOIUrl":"10.1016/j.vesic.2025.100095","url":null,"abstract":"<div><div>Platelet-derived extracellular vesicles (P-EV) are thought to facilitate the transfer of information from platelets to target cells, playing a role in both physiologic and pathophysiologic processes, particularly in regulating immune responses and healing processes. In addition, P-EV show promise as drug carriers and biomarkers for disease. However, the procedures for isolation, purification and fluorescent labeling of P-EV remain unstandardized. Moreover, the requirement to use freshly obtained platelets for generating EV presents a logistical challenge for their study. In this study, we isolated, characterized, and compared P-EV analogues by sonication of freshly obtained and lyophilized platelets, investigated fluorescent labeling methods, and monitored cellular uptake. We found that P-EV analogues derived from fresh or lyophilized platelets showed similar characteristics regarding size, surface proteins and content. Among the fluorescent labeling methods, CFSE and DiO-C6 were most effective in labeling P-EV analogues from both fresh and lyophilized platelets. All labeling methods led to an increase in P-EV analogue's size, with CFSE and DiO-C6 resulting in the smallest increase. The addition of P-EV analogues to cultured immortal endothelial cells revealed that P-EV analogues were effectively internalized and directed to the lysosomal compartment. The results indicate that P-EV analogues from lyophilized platelets have similar functional properties as those from freshly isolated platelets and these are retained after labeling with CFSE. Thus, lyophilized platelets can serve as a source of P-EV analogues for functional studies.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100095"},"PeriodicalIF":0.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-derived extracellular vesicles in the plasma of alpha-1 antitrypsin deficient individuals reveal pro-inflammatory metabolic and transcriptomic signatures","authors":"Zachary F. Greenberg ,&nbsp;Regina Oshins ,&nbsp;Karina Serban ,&nbsp;Sina F. Bazargani ,&nbsp;Timothy J. Garrett ,&nbsp;Nancy G. Casanova ,&nbsp;Joe GN. Garcia ,&nbsp;Mei He ,&nbsp;Nazli Khodayari","doi":"10.1016/j.vesic.2025.100093","DOIUrl":"10.1016/j.vesic.2025.100093","url":null,"abstract":"<div><div>Alpha-1 antitrypsin deficiency (AATD) is a genetic disorder caused by mutations in <em>SERPINA1</em>, leading to chronic obstructive pulmonary disease (COPD) and liver disease. Neutrophils are key regulators of inflammatory signaling networks; however, their dysregulation in AATD and the underlying molecular mechanisms remain poorly understood. Here, we employed a multi-omics approach integrating RNA sequencing (RNA-seq) and metabolomics to comprehensively characterize neutrophil dysfunction in AATD. RNA-seq analysis of blood neutrophils from AATD individuals revealed transcriptional dysregulation in genes involved in intracellular signaling, immune response regulation, and metabolic adaptation. Isolation and characterization of neutrophil-derived extracellular vesicles (EV) demonstrated an increased plasma burden of neutrophil elastase (NE)-rich EV with elevated surface-bound NE. Metabolomic profiling revealed that these EVs are enriched with pro-inflammatory metabolites linked to dysregulated signaling pathways. Integrated transcriptomic and metabolomic network analysis showed that altered neutrophil gene expression and signaling pathways reshape EV metabolic cargo, linking metabolic reprogramming to inflammatory signal transduction in AATD. Furthermore, differentially expressed EV metabolites may modulate gene expression in recipient cells, sustaining chronic inflammation in AATD. The observed upregulation of interferon, pattern recognition receptors, and cytokine-mediated signaling pathways in neutrophils suggests a potential feedback loop amplifying inflammation in AATD and COPD.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100093"},"PeriodicalIF":0.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outer membrane vesicles as novel therapeutics for heart repair","authors":"Ming Shen ,&nbsp;Dashuai Zhu ,&nbsp;Tongxuan Li ,&nbsp;Shixiong Wei ,&nbsp;Xianyun Wang ,&nbsp;Mingqi Zheng","doi":"10.1016/j.vesic.2025.100092","DOIUrl":"10.1016/j.vesic.2025.100092","url":null,"abstract":"<div><div>Ischemic heart disease is the leading cause of global morbidity and mortality. Amending an injured heart remains a major challenge in both clinics and basic research. Cardiac regenerative medicine that utilizes stem cells for heart repair and regeneration has transitioned into extracellular vesicles. Despite advancements in extracellular vesicle treatment for heart disease, the selection of parental cells and the transplantation pattern—whether autologous or allogeneic—remains a topic of ongoing debate due to immunological and therapeutic variability. Outer membrane vesicles (OMVs) offer an alternative option due to their unique properties, including large-scale production, and highly efficient drug loading/eluting, as well as proven modulation of pathological conditions in various diseases. Additionally, engineering strategies, including surface modification, cargo encapsulation, and microbiome modulation, enhance the specificity and safety of OMVs. At the joint of microbial engineering and cardiac regenerative medicine, OMVs represent a novel platform to develop precise therapeutics for heart disease treatment. This review underscores OMVs as innovative nanotherapeutic tools, bridging microbial-host interactions and cardiovascular health, with transformative potential for patient care.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel exosome-like vesicles from Dendrobium officinale: Unraveling a pioneering extraction protocol and their skin anti-aging potentials","authors":"Ye Zhang,&nbsp;Bo Zhao,&nbsp;Jing Wang,&nbsp;Meiping Shen,&nbsp;Zeyi Zhang,&nbsp;Chengjie Ren,&nbsp;Mimi Li,&nbsp;Melanie Liu,&nbsp;Zhicheng You,&nbsp;Ping Li","doi":"10.1016/j.vesic.2025.100090","DOIUrl":"10.1016/j.vesic.2025.100090","url":null,"abstract":"<div><h3>Objective</h3><div>Numerous studies suggest that exosome-like nanovesicles (EVs) derived from medicinal plants are crucial for their therapeutic and cosmetic benefits. Nevertheless, the precise role of EVs derived from <em>Dendrobium officinale</em> Kimura et Migo (<em>D. officinale</em>), a treasured traditional Chinese medicine, in promoting skin health has not been extensively studied. Thus, the objective of this study was to establish an effective isolation method to isolate these EVs and to investigate their potential anti-aging benefits for skin.</div></div><div><h3>Methods</h3><div>We developed a sequential filtration-based isolation process to extract <em>D</em>. <em>officinale</em> EVs (DO-EVs). We compared their physical properties and phytochemical profiles with those of EVs obtained via the standard ultracentrifugation method (UC-EVs) using Nanoparticle Tracking Analysis (NTA), Transmission Electron Microscopy (TEM), and LC-MS/MS. At the cellular level, we assessed the anti-aging efficacy of DO-EVs against UC-EVs and ethanol-extracted polysaccharides from <em>D. officinale</em> (EE-PS) by analyzing pro-COL1A2 levels, cytoskeletal organization, and senescence markers. We further examined DO-EVs’ anti-photodamage effects on UV-treated 3D skin models through histological and immunological staining, and elasticity measurements. Finally, we observed DO-EVs’ impact on human skin using a two-photon microscope.</div></div><div><h3>Results</h3><div>Both isolation methods yielded EVs of similar size and structure but distinct metabonomic profiles. DO-EVs uniquely contained compounds vital for skin health. Compared to UC-EVs and EE-PS, DO-EVs more effectively enhanced collagen I production, restored cytoskeletal structures, and reduced senescence markers in UV-exposed fibroblasts. In 3D skin models, DO-EVs significantly improved epidermal thickness and skin elasticity post-UV exposure, upregulated hyaluronic acid, collagen I &amp; IV, integrin α6β4, plectin, laminin 5, and nidogen expression, and decreased DNA damage. Human trials confirmed DO-EVs’ effectiveness in enhancing skin structures within two weeks.</div></div><div><h3>Conclusion</h3><div>Our isolation method successfully extracted <em>D. officinale</em> EVs with superior anti-aging bioactivity compared to UC-EVs or ethanol-extracted polysaccharides. These findings suggest DO-EVs’ great potential as an anti-aging cosmetic ingredient.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Head-to-head comparison of extracellular vesicles from different cell sources for cardiac repair” [Extracell Vesicle 5 (2025) 100068]","authors":"Kaiyue Zhang,&nbsp;Ke Cheng","doi":"10.1016/j.vesic.2025.100091","DOIUrl":"10.1016/j.vesic.2025.100091","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular engines driving biogenesis, trafficking and release of exosomes: SNARE proteins","authors":"Damilola Ogungbemi ,&nbsp;Susan Shorter ,&nbsp;Reza Asadollahi ,&nbsp;Stergios Boussios ,&nbsp;Saak V. Ovsepian","doi":"10.1016/j.vesic.2025.100089","DOIUrl":"10.1016/j.vesic.2025.100089","url":null,"abstract":"<div><div>Exosomes are extracellular vesicles released from cells, playing a crucial role in intercellular communication and contributing to fundamental processes underlying physiological and disease conditions. The growing recognition of the importance of exosomes in molecular signalling has spurred much interest in their biogenesis, transport and release, with SNARE proteins emerging as key players. Herein, we review advances in research of the role and mechanisms of SNAREs in the life journey of exosomes. We consider recent insights into the molecular machinery of exosome production and mobility, with reference to the role of various SNARE proteins. We discuss the mechanisms of SNARE interactions and their translational potential across multiple disease conditions. Elucidating the role of SNAREs in exosome biology presents a significant milestone in unravelling the molecular processes of intercellular communication and identifying new targets for translational research and therapeutic intervention.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cell-derived exosomes: emerging therapeutic strategies for cutaneous wound regeneration","authors":"Daqin Li ,&nbsp;Tong An ,&nbsp;Ning Wang ,&nbsp;Feifei Ma ,&nbsp;Tuo Li ,&nbsp;Ningning He ,&nbsp;Huijuan Song ,&nbsp;Qiang Liu","doi":"10.1016/j.vesic.2025.100088","DOIUrl":"10.1016/j.vesic.2025.100088","url":null,"abstract":"<div><div>The skin serves as the primary biological barrier protecting the organism from external environmental damage, including physical, chemical, and biohazardous agents such as ultraviolet radiation, mechanical trauma, pathogens, and radioactive substances. However, systemic diseases like diabetes can impede cutaneous regeneration, leading to delayed wound healing, which underscores the urgent need for novel therapeutic strategies. Exosomes, endogenous nanoscale vesicles, exhibit multifaceted biological functions, including intercellular communication, immunomodulation, tissue repair, and drug delivery. Notably, mesenchymal stem cells-derived exosomes (MSCs-Exo) have emerged as a promising candidate for cutaneous wound repair due to their low immunogenicity, absence of tumorigenic risk, and high accessibility. Current evidence indicates that MSCs-Exo and their engineered derivatives promote the healing of radiation-induced skin injuries by modulating macrophage polarization and suppressing inflammatory responses. Furthermore, their synergistic application with advanced biomaterials significantly enhances therapeutic efficacy. This review systematically summarizes recent advancements in exosome-based strategies for skin regeneration, highlights their integration with innovative biomaterials, and critically analyzes existing challenges and future translational research directions.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel mouse models for tracing brain-derived extracellular vesicles","authors":"Qi Wu ,&nbsp;Fanxiu Ma ,&nbsp;Meng Yuan ,&nbsp;Xiumei Jin ,&nbsp;Zhaodi Jiang ,&nbsp;Jiyan Ma","doi":"10.1016/j.vesic.2025.100087","DOIUrl":"10.1016/j.vesic.2025.100087","url":null,"abstract":"<div><div>Extracellular vesicles (EVs), particularly small extracellular vesicles (sEVs) such as exosomes, are carriers of biomolecules that reflect the metabolic status of host cells. Recently, sEVs have emerged as potential reservoirs of molecular biomarkers associated with pathological conditions, including cancers, neurodegenerative disorders, and cardiovascular diseases. While blood-based tests are common diagnostic tools, their efficacy in identifying central nervous system (CNS) diseases is significantly hindered by the blood-brain barrier, which prevents the entry of brain-derived proteins into the bloodstream. Capturing brain-derived sEVs (BDEs) in peripheral blood is a potential alternative for detecting CNS disease biomarkers. However, the presence of BDEs in blood remains obscure due to the lack of specific labeling. To address this challenge, we established two novel mouse models in which neuron-derived sEVs or EVs from specific brain regions were fluorescently labeled. Using these approaches we successfully detected fluorescently labeled EVs in both BDEs and plasma-derived sEVs (PDEs), confirming that BDEs are indeed present in peripheral blood. These models will serve as valuable tools for developing novel methods to efficiently capture BDEs in peripheral blood.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Unveiling the intricacies of bone homeostasis: Epigenetic regulation, Extracellular vesicles, and Angiogenesis integration” [Extracell Vesicle 3 (2024) 100042]","authors":"Célio Junior da Costa Fernandes","doi":"10.1016/j.vesic.2025.100085","DOIUrl":"10.1016/j.vesic.2025.100085","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}