Extracellular vesicle最新文献

{"title":"Mechanisms and potential of mesenchymal stem cell-derived exosomes for treating radiation-induced intestinal injury","authors":"Tong An ,&nbsp;Daqin Li ,&nbsp;Ning Wang ,&nbsp;Feifei Ma ,&nbsp;Tuo Li ,&nbsp;Ningning He ,&nbsp;Huijuan Song ,&nbsp;Qiang Liu","doi":"10.1016/j.vesic.2025.100078","DOIUrl":"10.1016/j.vesic.2025.100078","url":null,"abstract":"<div><div>Radiation-induced intestinal injury (RIII) is a prevalent complication of radiotherapy for abdominal and pelvic tumors, characterized by acute and chronic damage to intestinal tissues. Current treatments are primarily symptomatic, lacking effective targeted therapies. Mesenchymal stem cells (MSCs), due to their immunomodulatory and regenerative properties, and their derived exosomes, have emerged as promising therapeutic options for RIII. MSC-derived exosomes exhibit anti-inflammatory, antioxidant, and tissue-repairing properties, modulating immune responses and promoting intestinal barrier restoration. Engineering of exosomes further enhances their targeting and therapeutic efficiency. This review discusses the mechanisms and therapeutic potential of MSC-derived and engineered exosomes for RIII, emphasizing their role in reducing inflammation, promoting tissue repair, and maintaining microbial balance, while addressing future challenges and prospects for clinical translation.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100078"},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanisms and application prospects of exosomes in acute myocardial infarction","authors":"Peng Zhou ,&nbsp;Jia Zhang ,&nbsp;Leilei Zhang ,&nbsp;Jiangwei Yan ,&nbsp;Chen Fang","doi":"10.1016/j.vesic.2025.100073","DOIUrl":"10.1016/j.vesic.2025.100073","url":null,"abstract":"<div><div>Acute myocardial infarction (AMI) is a significant cause of death in cardiovascular diseases and an important topic in forensic pathology. It triggers various cellular pathological changes, leading to ventricular remodeling, changes in cardiac function, and, in severe cases, death. Current biomarkers for AMI lack specificity and can be influenced by various factors, making them challenging for diagnosis and differentiation. Exosomes, small vesicles with a lipid bilayer membrane, have emerged as potential biomarkers and therapeutic targets for AMI. They play a crucial role in intercellular cargo transport and communication. Exosomes play a dual role in cellular processes, exhibiting protective mechanisms under normal conditions while also contributing to disease progression in pathological contexts. This paper summarizes the mechanisms, research strategies, and application prospects of exosomes in AMI, providing insights for forensic investigations.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The functional extracellular vesicles target tumor microenvironment for gastrointestinal malignancies therapy","authors":"Dongqi Li ,&nbsp;Xiangyu Chu ,&nbsp;Yudong Ning ,&nbsp;Yinmo Yang ,&nbsp;Chen Wang ,&nbsp;Xiaodong Tian ,&nbsp;Yanlian Yang","doi":"10.1016/j.vesic.2025.100077","DOIUrl":"10.1016/j.vesic.2025.100077","url":null,"abstract":"<div><div>The tumor microenvironment (TME) represents a complex, heterogeneous ecosystem that significantly influences the progression of gastrointestinal (GI) cancers, comprising diverse cellular and non-cellular components. Although chemotherapeutic agents and targeted therapies offer partial benefits for patients with GI tumors, their efficacy remains limited due to the TME's complexity. Consequently, strategies to target and modulate the TME are critical to enhancing therapeutic outcomes. Extracellular vesicles (EVs) transport a wide array of biomolecules, including proteins, lipids, and nucleic acids, playing a pivotal role in intercellular communication and TME modulation. In recent years, EVs have gained attention as potential drug delivery vehicles, owing to their nanoscale size and capacity to shuttle bioactive molecules between cells and tissues. Moreover, engineered EVs hold promise for modulating the TME to treat GI cancers by improving targeting precision and tissue penetration. This review explores the latest strategies for the production and functionalization of EVs, along with advances in utilizing EVs for targeted therapy of the TME in GI tumor treatment.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100077"},"PeriodicalIF":0.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of extracellular vesicles in immune modulation, angiogenesis, progression and therapeutic resistance of glioblastoma","authors":"Muhammad Izhar,&nbsp;Ahed H. Kattaa,&nbsp;Amirhossein Akhavan-Sigari,&nbsp;Elaheh Shaghaghian,&nbsp;Yusuke S. Hori,&nbsp;Fred C. Lam,&nbsp;Deyaaldeen AbuReesh,&nbsp;Sara C. Emrich,&nbsp;Louisa Ustrzynski,&nbsp;Armine Tayag,&nbsp;Steven D. Chang,&nbsp;David J. Park","doi":"10.1016/j.vesic.2025.100075","DOIUrl":"10.1016/j.vesic.2025.100075","url":null,"abstract":"<div><div>Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor in adults, with a poor prognosis and high recurrence rates despite advancements in treatment. The tumor microenvironment (TME) of GBM is very complex and includes various cell types, such as immune cells, endothelial cells, astrocytes, and microglia. The TME plays a crucial role in the development of GBM and its resistance to therapy. One important part of the TME is extracellular vesicles (EVs), which help cells communicate and contribute to different aspects of GBM progression. They help the tumor grow and spread by increasing cellular proliferation, invasion, and survival. They also play a key role in angiogenesis by transferring pro-angiogenic factors to endothelial cells, which help form new blood vessels that provide the tumor with essential nutrients and oxygen. Within the context of immune modulation, EVs derived from GBM cells contain immunosuppressive molecules that alter the function of immune cells in the TME, enabling the tumor to escape immune surveillance. This immunosuppressive environment is marked by elevated levels of regulatory T cells (Tregs), myeloid-derived suppressor cells (MDSCs), and tumor-associated macrophages (TAMs). Furthermore, EVs contribute to therapeutic resistance by transferring drug-resistance factors from resistant to sensitive tumor cells, enhancing their capacity to withstand chemotherapy and radiotherapy. The RNA cargo of EVs, which includes microRNAs and long non-coding RNAs, plays a crucial role in modulating gene expression and cellular responses to treatment. In conclusion, EVs are vital in the development and progression of GBM by influencing angiogenesis, immune modulation, and therapeutic resistance. Targeting EV-mediated communication presents a promising therapeutic strategy for addressing the challenges posed by this deadly brain tumor.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling exosomal biomarkers in neurodegenerative diseases: LC-MS-based profiling","authors":"Yue Bi ,&nbsp;Liang Wang ,&nbsp;Chunyan Li ,&nbsp;Zhiying Shan ,&nbsp;Lanrong Bi","doi":"10.1016/j.vesic.2025.100071","DOIUrl":"10.1016/j.vesic.2025.100071","url":null,"abstract":"<div><div>Exosomes, small extracellular vesicles secreted by various cell types, play a critical role in intercellular communication and are increasingly recognized as key players in the progression of neurodegenerative diseases (NDs). Their ability to carry and propagate pathogenic proteins such as amyloid-beta, tau, and alpha-synuclein have established exosomal biomarkers as both key players in disease pathology and promising indicators for early diagnosis. Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful tool for the comprehensive analysis of exosomal cargo, enabling the identification of proteins, metabolites, and other molecules associated with neurodegeneration.</div><div>This review explores the structural composition, biogenesis, and role of exosomes in the propagation of pathogenic proteins in NDs such as Alzheimer's disease (AD), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS). It highlights the potential of exosomal biomarkers for disease diagnosis and monitoring. The foundation for LC-MS-based analyses is discussed, focusing on isolation, purification, and characterization techniques essential for reliable proteomic and metabolomic studies. The LC-MS workflow, from protein and metabolite identification to quantitative proteomics, is detailed alongside the advantages of LC-MS in uncovering exosomal biomarkers.</div><div>We delve into the application of LC-MS/MS in NDs research, showcasing its contributions to decoding disease pathology in AD, PD, and ALS by identifying specific exosomal biomarkers. Challenges such as the heterogeneity of exosome populations, variability in biofluid samples, and technical limitations in LC-MS analysis are critically examined. Finally, we discuss the future potential of LC-MS in advancing the diagnosis and treatment of NDs, emphasizing its transformative impact on biomarker discovery and personalized medicine.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100071"},"PeriodicalIF":0.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of Lycium barbarum-derived exosome-like nanovesicles in combating photodamage and enhancing skin barrier repair","authors":"Ye Zhang,&nbsp;Bo Zhao,&nbsp;Jing Wang,&nbsp;Zeyi Zhang,&nbsp;Meiping Shen,&nbsp;Chengjie Ren,&nbsp;Mimi Li,&nbsp;Melanie Liu,&nbsp;Zhicheng You,&nbsp;Ping Li","doi":"10.1016/j.vesic.2025.100072","DOIUrl":"10.1016/j.vesic.2025.100072","url":null,"abstract":"<div><div>In this study, the efficacy of <em>Lycium barbarum</em> L.-derived exosome-like nanovesicles (LB-EVs) in reinforcing skin barrier function were evaluated. Using a 3D skin model, expression levels of skin barrier proteins such as filaggrin, loricrin and claudin-1 were found to be modulated favorably upon treatment with LB-EVs, suggesting a role in maintaining cutaneous homeostasis. Additionally, we observed upregulated aquaporin 3 (AQP3) expression and increased natural moisturizing factor (NMF) components, indicating improved skin hydration. A split-face clinical trial involving individuals with sensitive skin revealed that incorporating LB-EVs into a cosmetic serum matrix led to reduced facial redness and transepidermal water loss (TEWL) compared to the control group. Two-photon imaging further demonstrated thickening of the stratum corneum on the treated side after 14 days of application, suggesting enhanced barrier integrity. The findings from this study highlight the potential of LB-EVs to fortify the skin's protective barrier, providing a novel therapeutic strategy for the management of photodamage and the enhancement of skin health.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100072"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143684140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Head-to-head comparison of extracellular vesicles from different cell sources for cardiac repair","authors":"Kaiyue Zhang,&nbsp;Ke Cheng","doi":"10.1016/j.vesic.2025.100068","DOIUrl":"10.1016/j.vesic.2025.100068","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100068"},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma extracellular vesicle cargo microRNAs are associated with heart failure and cardiovascular death following acute coronary syndrome","authors":"Worawan B. Limpitikul ,&nbsp;Michael G. Silverman ,&nbsp;Nedyalka Valkov ,&nbsp;Jeong-Gun Park ,&nbsp;Ashish Yeri ,&nbsp;Fernando Camacho Garcia ,&nbsp;Guoping Li ,&nbsp;Priyanka Gokulnath ,&nbsp;Marta Garcia-Contreras ,&nbsp;Eric Alsop ,&nbsp;Elizabeth Hutchins ,&nbsp;Michail Spanos ,&nbsp;Claire Lin ,&nbsp;Kriti Bomb ,&nbsp;Anthony Rosenzweig ,&nbsp;Raymond Kwong ,&nbsp;Kendall van-Keuren Jensen ,&nbsp;James L. Januzzi Jr. ,&nbsp;Ravi Shah ,&nbsp;David A. Morrow ,&nbsp;Saumya Das","doi":"10.1016/j.vesic.2025.100070","DOIUrl":"10.1016/j.vesic.2025.100070","url":null,"abstract":"<div><h3>Background and aims</h3><div>Patients with acute coronary syndromes (ACS) are at risk for long-term sequelae related to adverse ventricular remodeling including heart failure (HF) and cardiovascular death. Circulating microRNAs (miRNAs) have the potential to serve as biomarkers associated with the pathogenesis of ventricular remodeling. This study aims to identify and characterize plasma miRNAs associated with HF and cardiovascular death.</div></div><div><h3>Methods</h3><div>The association between 21 candidate miRNAs and HF or cardiovascular death was evaluated in 4541 patients from the Metabolic Efficiency with Ranolazine for Less Ischemia in Non-ST-Elevation Acute Coronary Syndromes (MERLIN)-TIMI 36 trial using samples from the time of index hospitalization for ACS. Associations between each miRNA and the composite endpoint of hospitalization for HF or cardiovascular death (HHF/CVD) were estimated in Cox proportional hazards models. The top candidate miRNAs were validated in an independent cohort of patients hospitalized for ACS using complementary methods for isolation of extracellular RNA carriers.</div></div><div><h3>Results</h3><div>Over 12 months in MERLIN-TIMI 36, 313 individuals met the primary endpoint. In total, 11 miRNAs were associated with HHF/CVD. After adjustment for clinical factors and established biomarkers, miR-223-3p (hazard ratio 0.81 [95% confidence interval: 0.60–1.09], 0.62 [0.45–0.87], and 0.61 [0.43–0.85] for the 2nd, 3rd, and 4th quartiles, respectively) and miR-378c (0.96 [0.65–1.42], 1.14 [0.78–1.66], and 1.39 [0.97–1.98], for the 2nd, 3rd, and 4th quartiles, respectively) were independently associated with the risk of HHF/CVD. In an independent validation cohort of 27 patients hospitalized with ACS, we found that both miRNAs were identified in different RNA-carrying extracellular particles in plasma, including extracellular vesicles (EVs) and nonvesicular extracellular nanoparticles (NVEPs) including exomeres and supermeres. Both miR-223-3p and 378c were more enriched in small EVs (sEVs) compared to NVEPs. In concordance with the direction of the associations observed in MERLIN-TIMI 36 with HHF, the level of miR-223-3p in sEVs and exomeres was positively associated with left ventricular ejection fraction (LVEF) post-ACS while the level of miR-378c in sEVs was negatively associated with LVEF in the acute phase.</div></div><div><h3>Conclusion</h3><div>Circulating miRNAs 223-3p and 378c were associated with the risk of HHF/CVD after adjustment for clinical factors and established cardiovascular biomarkers. Carrier-specific measurement of these miRNAs may emerge as novel minimally invasive biomarkers for post-ACS cardiac remodeling and shed light on potentially new targetable pathways.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100070"},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for isolating small extracellular vesicles from parenchymal tissues","authors":"Yanan Sun ,&nbsp;Junqing An ,&nbsp;Jie Yuan ,&nbsp;Chaoshan Han ,&nbsp;Gangjian Qin","doi":"10.1016/j.vesic.2025.100069","DOIUrl":"10.1016/j.vesic.2025.100069","url":null,"abstract":"<div><div>Extracellular vesicles (EV) are released by almost all cells into the extracellular space, where they navigate through the interstitium or transfer bioactive molecules into neighboring cells, thereby regulating tissue homeostasis. Small EV (sEV) generally refer to EV with a diameter of less than 200 nm, which include exosomes formed in the endosomal system and small ectosomes assembled at and released from the plasma membrane. While techniques for isolating sEV from large body fluids auch as blood and urine are relatively well-developed, isolating sEV from solid organs remains challenging. This is because the preparations are often seen contaminated with <em>intracellular</em> vesicles due to cell breakage resulting from the treatments used to release sEV from the extracellular matrix. Here, we introduce a new gentle method that allows for the reliable isolation of sEV from heart and liver tissues with high purity and yield. The protocol involves an initial treatment of tissues with selected collagenase, followed by consecutive differential centrifugation and density-gradient ultracentrifugation to separate sEV from cells and large EV (lEV), and further density-gradient ultracentrifugation to fractionate sEV subpopulations. Characterization of the preparations reveals markedly enhanced cellular survival and reduced co-purification of extracellular non-vesicular particles as well as subcellular organelles. Moreover, we found that sEV isolated from heart, liver, and plasma are similar in morphology and size, but differ in density and protein marker distributions among sEV subpopulations. Our method may facilitate the isolation of sEV from solid tissues with better quality for further molecular characterization and functional studies.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100069"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomal delivery of IL-10: Biodistribution, pharmacokinetics, and preterm birth prevention strategies","authors":"Brinley Harrington ,&nbsp;Tilu Jain Thomas ,&nbsp;Madhuri Tatiparthy,&nbsp;Awanit Kumar,&nbsp;Lauren Richardson,&nbsp;Ramkumar Menon,&nbsp;Ananth Kumar Kammala","doi":"10.1016/j.vesic.2025.100066","DOIUrl":"10.1016/j.vesic.2025.100066","url":null,"abstract":"<div><div>This study investigates the potential of extracellular vesicles (EVs) loaded with interleukin-10 (IL-10) to reduce infection-induced preterm birth (PTB). IL-10 has shown promise in reducing PTB by dampening inflammatory responses, but challenges exist with intraamniotic administration. The study evaluates IL-10 gene-transfected cell-produced EVs containing IL-10 (tIL-10), comparing them with recombinant IL-10 (rIL-10) and rIL-10 incorporated in EVs via electroporation (eIL-10). Characterization of tIL-10 includes size, shape, and molecular markers. Functional assays demonstrate tIL-10's ability to reduce pro-inflammatory cytokine production and extend half-life, with biodistribution in maternal and fetal tissues. The study findings indicate that tIL-10 displays an average size of 108.7 ± 20.5 nm, round with a diameter of ∼0.12 μm, and expresses EV markers CD9 and CD81, containing IL-10 cargo. In vitro, LPS stimulation demonstrates that tIL-10 significantly reduces the production of pro-inflammatory cytokines IL-6 and IL-8 from maternal decidual cells. Biodistribution studies reveal tIL-10 presence in placental and fetal membranes within 5 min, persisting for up to 3 h. Pharmacokinetic studies using non-compartmental analysis of plasma data, and the linear trapezoidal method establish key pharmacologic parameters for each drug. Pharmacological findings establish eIL-10 and tIL-10's ability to significantly delay PTB onset after E. coli exposure. These findings underscore the potential of tIL-10 as an effective therapeutic agent for PTB, with implications for clinical practice and further research in reproductive pharmacology.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"5 ","pages":"Article 100066"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143103009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}