Extracellular vesicle最新文献

{"title":"Erratum to “MicroRNAs won the Nobel Prize. Now, can extracellular vesicles help them become drugs?” [Extracell Vesicle 5 (2025) 100080]","authors":"Vanessa YiRan Li , Nicole Rosas , Sharon Fleischer , Gordana Vunjak-Novakovic , Ke Cheng","doi":"10.1016/j.vesic.2025.100086","DOIUrl":"10.1016/j.vesic.2025.100086","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular engines driving biogenesis, trafficking and release of exosomes: SNARE proteins","authors":"Damilola Ogungbemi ,&nbsp;Susan Shorter ,&nbsp;Reza Asadollahi ,&nbsp;Stergios Boussios ,&nbsp;Saak V. Ovsepian","doi":"10.1016/j.vesic.2025.100089","DOIUrl":"10.1016/j.vesic.2025.100089","url":null,"abstract":"<div><div>Exosomes are extracellular vesicles released from cells, playing a crucial role in intercellular communication and contributing to fundamental processes underlying physiological and disease conditions. The growing recognition of the importance of exosomes in molecular signalling has spurred much interest in their biogenesis, transport and release, with SNARE proteins emerging as key players. Herein, we review advances in research of the role and mechanisms of SNAREs in the life journey of exosomes. We consider recent insights into the molecular machinery of exosome production and mobility, with reference to the role of various SNARE proteins. We discuss the mechanisms of SNARE interactions and their translational potential across multiple disease conditions. Elucidating the role of SNAREs in exosome biology presents a significant milestone in unravelling the molecular processes of intercellular communication and identifying new targets for translational research and therapeutic intervention.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulating extracellular vesicles with a minimal RISC complex as novel gene silencing tool","authors":"Tao Qiu,&nbsp;Yu Yan,&nbsp;Rui Hu,&nbsp;Yuan Yi,&nbsp;Guowu Liu,&nbsp;Wenqiang Lu,&nbsp;Xin Zhou,&nbsp;Ke Xu","doi":"10.1016/j.vesic.2025.100094","DOIUrl":"10.1016/j.vesic.2025.100094","url":null,"abstract":"<div><div>Gene silencing modalities including small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs) have prospered in both fundamental research and clinical translations in recent years, with delivery platform being one of the key elements for success. Extracellular vesicles (EVs) as natural carriers for cell-cell communication have been engineered in a variety of ways as delivery platform for gene silencing, yet facing limited efficiency and reproducibility. In this study, we developed a new strategy for engineering EVs as gene silencing tool. A minimal RNA-induced silencing complex (RISC), composing of modified Argonaute 2 (AGO2) protein and specially designed guide strand RNAs, were encapsulated into EVs and elicited prominent EGFP silencing in proof-of-concept study. This modular EVs platform, which we named as minRISC-EVs, efficiently silenced iNOS expression in M1 macrophages as well as STAT6/A20 expression in M2 macrophages, enabling macrophages polarization towards desired directions. The macrophage modulating ability was further validated <em>in vivo</em>, as minRISC-EVs against iNOS alleviated mice lung inflammation in lipopolysaccharide (LPS)-induced acute lung injury model, and minRISC-EVs against STAT6/A20 inhibited B16F10 tumor progression in the tumor xenograft model. In summary, minRISC-EVs can be utilized as novel gene silencing tool, and hold great promise for clinical translation in the future.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100094"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicle-based targeted RNA therapies against cancer","authors":"Ziqi Wang ,&nbsp;Haonan Xing ,&nbsp;Yuanyu Huang ,&nbsp;Mei Lu","doi":"10.1016/j.vesic.2025.100083","DOIUrl":"10.1016/j.vesic.2025.100083","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) are nanoscale vesicles released by cells and serve as natural carriers for RNAs, DNAs, proteins, and lipids that mediate intercellular communication. EV application as nanocarriers in targeted cancer therapy has gained significant attention. The delivery of RNAs via EVs has emerged as a promising technology in the past few decades, as EVs can encapsulate RNAs to protect them from degradation and enhance their uptake by recipient cells. Notably, chemical or genetic modifications to the surface of EVs can further strengthen their targeting ability. These advancements not only improve the specificity of RNA therapies but also address the challenges of RNA delivery associated with traditional methods. This review discusses the recent advancements in the delivery of messenger RNAs (mRNAs), miRNAs, siRNAs, and other RNA species for targeted cancer therapy via EVs. We aim to provide critical insights into the strategic design of advanced EV-based nanoplatforms for RNA delivery.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outer membrane vesicles as novel therapeutics for heart repair","authors":"Ming Shen ,&nbsp;Dashuai Zhu ,&nbsp;Tongxuan Li ,&nbsp;Shixiong Wei ,&nbsp;Xianyun Wang ,&nbsp;Mingqi Zheng","doi":"10.1016/j.vesic.2025.100092","DOIUrl":"10.1016/j.vesic.2025.100092","url":null,"abstract":"<div><div>Ischemic heart disease is the leading cause of global morbidity and mortality. Amending an injured heart remains a major challenge in both clinics and basic research. Cardiac regenerative medicine that utilizes stem cells for heart repair and regeneration has transitioned into extracellular vesicles. Despite advancements in extracellular vesicle treatment for heart disease, the selection of parental cells and the transplantation pattern—whether autologous or allogeneic—remains a topic of ongoing debate due to immunological and therapeutic variability. Outer membrane vesicles (OMVs) offer an alternative option due to their unique properties, including large-scale production, and highly efficient drug loading/eluting, as well as proven modulation of pathological conditions in various diseases. Additionally, engineering strategies, including surface modification, cargo encapsulation, and microbiome modulation, enhance the specificity and safety of OMVs. At the joint of microbial engineering and cardiac regenerative medicine, OMVs represent a novel platform to develop precise therapeutics for heart disease treatment. This review underscores OMVs as innovative nanotherapeutic tools, bridging microbial-host interactions and cardiovascular health, with transformative potential for patient care.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cell-derived exosomes: emerging therapeutic strategies for cutaneous wound regeneration","authors":"Daqin Li ,&nbsp;Tong An ,&nbsp;Ning Wang ,&nbsp;Feifei Ma ,&nbsp;Tuo Li ,&nbsp;Ningning He ,&nbsp;Huijuan Song ,&nbsp;Qiang Liu","doi":"10.1016/j.vesic.2025.100088","DOIUrl":"10.1016/j.vesic.2025.100088","url":null,"abstract":"<div><div>The skin serves as the primary biological barrier protecting the organism from external environmental damage, including physical, chemical, and biohazardous agents such as ultraviolet radiation, mechanical trauma, pathogens, and radioactive substances. However, systemic diseases like diabetes can impede cutaneous regeneration, leading to delayed wound healing, which underscores the urgent need for novel therapeutic strategies. Exosomes, endogenous nanoscale vesicles, exhibit multifaceted biological functions, including intercellular communication, immunomodulation, tissue repair, and drug delivery. Notably, mesenchymal stem cells-derived exosomes (MSCs-Exo) have emerged as a promising candidate for cutaneous wound repair due to their low immunogenicity, absence of tumorigenic risk, and high accessibility. Current evidence indicates that MSCs-Exo and their engineered derivatives promote the healing of radiation-induced skin injuries by modulating macrophage polarization and suppressing inflammatory responses. Furthermore, their synergistic application with advanced biomaterials significantly enhances therapeutic efficacy. This review systematically summarizes recent advancements in exosome-based strategies for skin regeneration, highlights their integration with innovative biomaterials, and critically analyzes existing challenges and future translational research directions.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144662925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to “Head-to-head comparison of extracellular vesicles from different cell sources for cardiac repair” [Extracell Vesicle 5 (2025) 100068]","authors":"Kaiyue Zhang,&nbsp;Ke Cheng","doi":"10.1016/j.vesic.2025.100091","DOIUrl":"10.1016/j.vesic.2025.100091","url":null,"abstract":"","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel exosome-like vesicles from Dendrobium officinale: Unraveling a pioneering extraction protocol and their skin anti-aging potentials","authors":"Ye Zhang,&nbsp;Bo Zhao,&nbsp;Jing Wang,&nbsp;Meiping Shen,&nbsp;Zeyi Zhang,&nbsp;Chengjie Ren,&nbsp;Mimi Li,&nbsp;Melanie Liu,&nbsp;Zhicheng You,&nbsp;Ping Li","doi":"10.1016/j.vesic.2025.100090","DOIUrl":"10.1016/j.vesic.2025.100090","url":null,"abstract":"<div><h3>Objective</h3><div>Numerous studies suggest that exosome-like nanovesicles (EVs) derived from medicinal plants are crucial for their therapeutic and cosmetic benefits. Nevertheless, the precise role of EVs derived from <em>Dendrobium officinale</em> Kimura et Migo (<em>D. officinale</em>), a treasured traditional Chinese medicine, in promoting skin health has not been extensively studied. Thus, the objective of this study was to establish an effective isolation method to isolate these EVs and to investigate their potential anti-aging benefits for skin.</div></div><div><h3>Methods</h3><div>We developed a sequential filtration-based isolation process to extract <em>D</em>. <em>officinale</em> EVs (DO-EVs). We compared their physical properties and phytochemical profiles with those of EVs obtained via the standard ultracentrifugation method (UC-EVs) using Nanoparticle Tracking Analysis (NTA), Transmission Electron Microscopy (TEM), and LC-MS/MS. At the cellular level, we assessed the anti-aging efficacy of DO-EVs against UC-EVs and ethanol-extracted polysaccharides from <em>D. officinale</em> (EE-PS) by analyzing pro-COL1A2 levels, cytoskeletal organization, and senescence markers. We further examined DO-EVs’ anti-photodamage effects on UV-treated 3D skin models through histological and immunological staining, and elasticity measurements. Finally, we observed DO-EVs’ impact on human skin using a two-photon microscope.</div></div><div><h3>Results</h3><div>Both isolation methods yielded EVs of similar size and structure but distinct metabonomic profiles. DO-EVs uniquely contained compounds vital for skin health. Compared to UC-EVs and EE-PS, DO-EVs more effectively enhanced collagen I production, restored cytoskeletal structures, and reduced senescence markers in UV-exposed fibroblasts. In 3D skin models, DO-EVs significantly improved epidermal thickness and skin elasticity post-UV exposure, upregulated hyaluronic acid, collagen I &amp; IV, integrin α6β4, plectin, laminin 5, and nidogen expression, and decreased DNA damage. Human trials confirmed DO-EVs’ effectiveness in enhancing skin structures within two weeks.</div></div><div><h3>Conclusion</h3><div>Our isolation method successfully extracted <em>D. officinale</em> EVs with superior anti-aging bioactivity compared to UC-EVs or ethanol-extracted polysaccharides. These findings suggest DO-EVs’ great potential as an anti-aging cosmetic ingredient.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144779545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using common features of viruses and EVs for a novel EV-based lateral flow test for HIV","authors":"Casey Scott-Weathers ,&nbsp;Kaitlyn King ,&nbsp;Gary Baisa ,&nbsp;John Hural ,&nbsp;Kimberly Luke","doi":"10.1016/j.vesic.2025.100084","DOIUrl":"10.1016/j.vesic.2025.100084","url":null,"abstract":"<div><div>The similarities between extracellular vesicles (EVs) and viruses make them challenging to distinguish and separate into unique populations. A novel lateral flow test has been developed by utilizing these shared properties to improve at-home testing for HIV. By targeting EVs released from HIV infected cells (HIV-EVs) and HIV virus particles for immunocapture, which share common transmembrane proteins like tetraspanins, HIV proteins can be concentrated from blood samples in a lateral flow device. Others have described immunocapture of EVs by lateral flow; here we describe capture and lysis for downstream detection of specific HIV cargo for a sensitive antigen-only HIV test. We found that HIV antigens p24 and Nef are detected early in infection and may significantly improve the sensitivity of an at-home test format by utilizing EVs as a novel reservoir of HIV antigens.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100084"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic characterization of mammalian extracellular vesicles using nano-flow cytometry","authors":"Benjamin T. Vyzourek ,&nbsp;Dirk Anderson ,&nbsp;Luke Skrabal ,&nbsp;Christine E. Humphrey ,&nbsp;Eduardo Romero ,&nbsp;Brittany Schweiger ,&nbsp;Forrest Kievit ,&nbsp;Jeremy R. Miles ,&nbsp;Angela K. Pannier","doi":"10.1016/j.vesic.2025.100098","DOIUrl":"10.1016/j.vesic.2025.100098","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) are nanoscale, membrane-enclosed particles that transport bioactive cargo between cells and are increasingly studied for their potential in diagnostic and therapeutic applications. Advancing EV-based technologies for these applications depend on the ability to consistently isolate and characterize vesicle populations with defined biophysical and molecular properties. Efforts to obtain pure EV populations from cell culture systems are limited by inherent EV heterogeneity, exogenous particle contamination introduced by media supplements, and the co-isolation of non-vesicular contaminants. These challenges are further compounded by the limitations of conventional EV characterization platforms, which often lack the resolution to distinguish EVs from similarly sized non-vesicular particles or to capture molecular heterogeneity at the single-vesicle scale. Together, these limitations highlight the need for analytical approaches capable of resolving EV heterogeneity and enabling comparisons across EV production conditions and isolation strategies. In this study, we used nano-flow cytometry (nFCM) for high-resolution analysis of individual EVs, enabling simultaneous measurement of particle size, concentration, and tetraspanin expression. This approach revealed substantial amounts of exogenous particle contamination in media supplements commonly used to culture EV-producing cells, and quantified differences in EV purity and yield between methods used to isolate EVs from the media of the producing cells. Additionally, analysis of EVs derived from HEK293T, U-87 MG, and hMSC mammalian cell cultures revealed cell type-specific differences in EV production and expression of tetraspanin markers CD9, CD63, and CD81. Collectively, these results demonstrate that careful selection of media compositions and isolation strategies, combined with nFCM analytical techniques can resolve biological differences in EV populations.</div></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"6 ","pages":"Article 100098"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145519278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}