Advances in applied microbiology最新文献_第4页

CRISPR/Cas genome editing systems in thermophiles: Current status, associated challenges, and future perspectives. 嗜热菌CRISPR/Cas基因组编辑系统:现状、相关挑战和未来展望

2区生物学

Advances in applied microbiology Pub Date : 2022-02-25 DOI: 10.1016/bs.aambs.2022.02.001

Yilin Le,Jianzhong Sun

{"title":"CRISPR/Cas genome editing systems in thermophiles: Current status, associated challenges, and future perspectives.","authors":"Yilin Le,Jianzhong Sun","doi":"10.1016/bs.aambs.2022.02.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.02.001","url":null,"abstract":"Thermophiles, offering an attractive and unique platform for a broad range of applications in biofuels and environment protections, have received a significant attention and growing interest from academy and industry. However, the exploration and exploitation of thermophilic organisms have been hampered by the lack of a powerful genome manipulation tool to improve production efficiency. At current, the clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR associated (Cas) system has been successfully exploited as a competent, simplistic, and powerful tool for genome engineering both in eukaryotes and prokaryotes. Indeed, with the significant efforts made in recent years, some thermostable Cas9 proteins have been well identified and characterized and further, some thermostable Cas9-based editing tools have been successfully established in some representative obligate thermophiles. In this regard, we reviewed the current status and its progress in CRISPR/Cas-based genome editing system towards a variety of thermophilic organisms. Despite the potentials of these progresses, multiple factors/barriers still have to be overcome and optimized for improving its editing efficiency in thermophiles. Some insights into the roles of thermostable CRISPR/Cas technologies for the metabolic engineering of thermophiles as a thermophilic microbial cell factory were also fully analyzed and discussed.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138508783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Advances in microbial synthesis of bioplastic monomers. 微生物合成生物塑料单体的研究进展。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.05.002

Jie Liu, Jianmin Liu, Liang Guo, Jia Liu, Xiulai Chen, Liming Liu, Cong Gao

引用次数: 0

Microbial communities of halite deposits and other hypersaline environments. 盐岩沉积物和其他高盐环境的微生物群落。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.06.001

Thomas P Thompson, Julianne Megaw, Stephen A Kelly, Jason Hopps, Brendan F Gilmore

{"title":"Microbial communities of halite deposits and other hypersaline environments.","authors":"Thomas P Thompson, Julianne Megaw, Stephen A Kelly, Jason Hopps, Brendan F Gilmore","doi":"10.1016/bs.aambs.2022.06.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.06.001","url":null,"abstract":"Large regions of Earth's surface are underlain by salt deposits that evaporated from ancient oceans and are populated by extreme halophilic microbes. While the microbiology of ancient evaporites has been well studied, the ecology of halite deposits and more recently formed NaCl \"salticle\" stalactite structures (speleothems) in a Triassic halite mine are less well characterized. The microbiome of Kilroot Salt Mine was profiled using conventional and enhanced culturing techniques. From this, 89 halophilic archaeal isolates from six known genera, and 55 halophilic or halotolerant bacterial isolates from 18 genera were obtained. Culture-independent metagenomic approaches also revealed that culturing techniques were inadvertently biased toward specific taxa, and the need for optimized isolation procedures are required to enhance cultivation diversity. Speleothems formed from saturated brines are unique structures that have the potential to entomb haloarchaea cells for thousands of years within fluid inclusions. The presence of such fluid inclusions, alongside the high abundance of genes related to glycerol metabolism, biofilm formation, and persister cell formation is highly suggestive of an environmental niche that could promote longevity and survivability. Finally, previous studies reporting the discovery of novel biocatalysts from the Kilroot mine microbiome, suggests that this environment may be an untapped source of chemical diversity with high biodiscovery potential.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9122277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal activity of lactic acid bacteria and their application in food biopreservation. 乳酸菌抗真菌活性及其在食品生物保鲜中的应用。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.07.001

Houssam Abouloifa, Ismail Hasnaoui, Yahya Rokni, Reda Bellaouchi, Nabil Ghabbour, Salwa Karboune, Milena Brasca, Abdelkarim Abousalham, Bassem Jaouadi, Ennouamane Saalaoui, Abdeslam Asehraou

{"title":"Antifungal activity of lactic acid bacteria and their application in food biopreservation.","authors":"Houssam Abouloifa, Ismail Hasnaoui, Yahya Rokni, Reda Bellaouchi, Nabil Ghabbour, Salwa Karboune, Milena Brasca, Abdelkarim Abousalham, Bassem Jaouadi, Ennouamane Saalaoui, Abdeslam Asehraou","doi":"10.1016/bs.aambs.2022.07.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.07.001","url":null,"abstract":"Lactic acid bacteria (LAB) are ubiquitous bacteria associated with spontaneous lactic fermentation of vegetables, dairy and meat products. They are generally recognized as safe (GRAS), and they are involved in transformation of probiotic lacto-fermented foods, highly desired for their nutraceutical properties. The antifungal activity is one of the exciting properties of LAB, because of its possible application in food bio-preservation, as alternative to chemical preservatives. Many recent research works have been developed on antifungal activity of LAB, and they demonstrate their capacity to produce various antifungal compounds, (i.e. organic acids, PLA, proteinaceous compounds, peptides, cyclic dipeptides, fatty acids, and other compounds), of different properties (hydrophilic, hydrophobic and amphiphilic). The effectiveness of LAB in controlling spoilage and pathogenic fungi, demonstrated in different agricultural and food products, can be due to the synergistic effect between their antifungal compounds of different properties; where the amphiphilic-compounds allow the contact between the target microbial cell (hydrophilic compartment) and antifungal hydrophobic-compounds. Further studies on the interaction between compounds of these three properties are to de be developed, in order to highlight more their mechanism of action, and make LAB more profitable in improving shelf life and nutraceutical properties of foods.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9122278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Role of psychrotrophic bacteria and cold-active enzymes in composting methods adopted in cold regions. 冷养细菌和冷活性酶在寒冷地区堆肥方法中的作用。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.08.001

Vivek Manyapu, Ayush Lepcha, Sanjeev Kumar Sharma, Rakshak Kumar

引用次数: 0

Gain-of-function research. 功能的研究。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.06.002

K P Saalbach

{"title":"Gain-of-function research.","authors":"K P Saalbach","doi":"10.1016/bs.aambs.2022.06.002","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.06.002","url":null,"abstract":"The term Gain-of-Function (GoF) describes the gain of new functions by organisms through genetic changes, which can naturally occur or by experimental genetic modifications. Gain-of-Function research on viruses is enhancing transmissibility, virus replication, virulence, host range, immune evasion or drug and vaccine resistance to get insights into the viral mechanisms, to create and analyze animal models, to accelerate drug and vaccine development and to improve pandemic preparedness. A subset is the GoF research of concern (GOFROC) on enhanced potentially pandemic pathogens (ePPPs) that could be harmful for humans. A related issue is the military use of research as dual-use research of concern (DURC). Influenza and coronaviruses are main research targets, because they cause pandemics by airborne infections. Two studies on avian influenza viruses initiated a global debate and a temporary GoF pause in the United States which ended with a new regulatory framework in 2017. In the European Union and China, GoF and DURC are mainly covered by the legislation for laboratory safety and genetically modified organisms. After the coronavirus outbreaks, the GoF research made significant advances, including analyses of modified MERS-like and SARS-like viruses and the creation of synthetic SARS-CoV-2 viruses as a platform to generate mutations. The GoF research on viruses will still play an important role in future, but the need to clarify the differences and overlaps between GoF research, GOFROC and DURC and the need for specialized oversight authorities are still debated.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10626343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Advances in microbial production of feed amino acid. 饲料氨基酸微生物生产研究进展。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.05.001

Kuo Zhao, Jianmin Liu, Cong Gao, Jia Liu, Xiulai Chen, Liming Liu, Liang Guo

引用次数: 1

Green synthesis of nanoparticles by probiotics and their application. 利用益生菌绿色合成纳米颗粒及其应用。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.05.003

Lei Qiao, Xina Dou, Xiaofan Song, Chunlan Xu

{"title":"Green synthesis of nanoparticles by probiotics and their application.","authors":"Lei Qiao, Xina Dou, Xiaofan Song, Chunlan Xu","doi":"10.1016/bs.aambs.2022.05.003","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.05.003","url":null,"abstract":"Nanoparticles (NPs), which have unique properties due to their extremely small size and high surface area to volume ratio, have attracted considerable attention and become an important tool for innovation in various fields. Traditionally, NPs are synthesized by physical and chemical processes, but these methods have high capital costs and energy demand, and involve the use of toxic and hazardous chemicals, which are prone to secondary pollution of the environment. In recent years, the use of microorganism-mediated methods has emerged as an alternative to traditional physical and chemical methods. The synthesis of NPs by microorganism has the advantages of non-toxicity, eco-friendliness, low-cost, reproducibility in production, easy amplification, and well-defined morphology. Probiotics are a kind of active microorganisms beneficial to the host. Compared with other microorganisms, probiotics are characterized by non-pathogenicity, rapid growth and regulation of gene expression, and produce a variety of proteins and enzymes involved in the synthesis of NPs. Therefore, the production of NPs using probiotics is an environmentally friendly and commercially attractive method, which provides new ideas and approaches for the application of NPs in the fields of biomedicine, agriculture and environmental remediation. This review aims to summarize the literature on the biosynthesis of NPs by probiotics and their synthetic mechanisms and applications.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10358042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The use of the electromagnetic field in microbial process bioengineering. 电磁场在微生物过程生物工程中的应用。

2区生物学

Advances in applied microbiology Pub Date : 2022-01-01 DOI: 10.1016/bs.aambs.2022.08.002

Joanna Jabłońska, Kamila Dubrowska, Marta Gliźniewicz, Oliwia Paszkiewicz, Adrian Augustyniak, Bartłomiej Grygorcewicz, Maciej Konopacki, Agata Markowska-Szczupak, Marian Kordas, Barbara Dołęgowska, Rafał Rakoczy

{"title":"The use of the electromagnetic field in microbial process bioengineering.","authors":"Joanna Jabłońska, Kamila Dubrowska, Marta Gliźniewicz, Oliwia Paszkiewicz, Adrian Augustyniak, Bartłomiej Grygorcewicz, Maciej Konopacki, Agata Markowska-Szczupak, Marian Kordas, Barbara Dołęgowska, Rafał Rakoczy","doi":"10.1016/bs.aambs.2022.08.002","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.08.002","url":null,"abstract":"An electromagnetic field (EMF) has been shown to have various stimulatory or inhibitory effects on microorganisms. Over the years, growing interest in this topic led to numerous discoveries suggesting the potential applicability of EMF in biotechnological processes. Among these observations are stimulative effects of this physical influence resulting in intensified biomass production, modification of metabolic activity, or pigments secretion. In this review, we present the current state of the art and underline the main findings of the application of EMF in bioprocessing and their practical meaning in process engineering using examples selected from studies on bacteria, archaea, microscopic fungi and yeasts, viruses, and microalgae. All biological data are presented concerning the classification of EMF. Furthermore, we aimed to highlight missing parts of contemporary knowledge and indicate weak spots in the approaches found in the literature.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10638086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Uninvited Guests: a Chronology of Petri Dish Contaminations. 不速之客:培养皿污染年表。

2区生物学

Advances in applied microbiology Pub Date : 2021-01-01 Epub Date: 2021-05-27 DOI: 10.1016/bs.aambs.2021.04.002

Gilbert Shama

引用次数: 1