Advances in applied microbiology最新文献_第4页

Halotolerance mechanisms in salt‑tolerant cyanobacteria. 耐盐蓝藻的耐盐机制。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 Epub Date: 2023-07-23 DOI: 10.1016/bs.aambs.2023.07.003

Hakuto Kageyama, Rungaroon Waditee-Sirisattha

{"title":"Halotolerance mechanisms in salt‑tolerant cyanobacteria.","authors":"Hakuto Kageyama, Rungaroon Waditee-Sirisattha","doi":"10.1016/bs.aambs.2023.07.003","DOIUrl":"10.1016/bs.aambs.2023.07.003","url":null,"abstract":"Cyanobacteria are ubiquitously distributed in nature and are the most abundant photoautotrophs on Earth. Their long evolutionary history reveals that cyanobacteria have a remarkable capacity and strong adaptive tendencies to thrive in a variety of conditions. Thus, they can survive successfully, especially in harsh environmental conditions such as salty environments, high radiation, or extreme temperatures. Among others, salt stress because of excessive salt accumulation in salty environments, is the most common abiotic stress in nature and hampers agricultural growth and productivity worldwide. These detrimental effects point to the importance of understanding the molecular mechanisms underlying the salt stress response. While it is generally accepted that the stress response mechanism is a complex network, fewer efforts have been made to represent it as a network. Substantial evidence revealed that salt-tolerant cyanobacteria have evolved genomic specific mechanisms and high adaptability in response to environmental changes. For example, extended gene families and/or clusters of genes encoding proteins involved in the adaptation to high salinity have been collectively reported. This chapter focuses on recent advances and provides an overview of the molecular basis of halotolerance mechanisms in salt‑tolerant cyanobacteria as well as multiple regulatory pathways. We elaborate on the major protective mechanisms, molecular mechanisms associated with halotolerance, and the global transcriptional landscape to provide a gateway to uncover gene regulation principles. Both knowledge and omics approaches are utilized in this chapter to decipher the mechanistic insights into halotolerance. Collectively, this chapter would have a profound impact on providing a comprehensive understanding of halotolerance in salt‑tolerant cyanobacteria.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"124 ","pages":"55-117"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10034060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction and application of extracellular polymeric substances from fungi. 真菌胞外聚合物物质的提取和应用。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 Epub Date: 2023-08-23 DOI: 10.1016/bs.aambs.2023.08.001

Sijia Wu, Hongxun Huo, Yixiao Shi, Feiran Zhang, Tingting Gu, Zhen Li

{"title":"Extraction and application of extracellular polymeric substances from fungi.","authors":"Sijia Wu, Hongxun Huo, Yixiao Shi, Feiran Zhang, Tingting Gu, Zhen Li","doi":"10.1016/bs.aambs.2023.08.001","DOIUrl":"10.1016/bs.aambs.2023.08.001","url":null,"abstract":"Extracellular polymeric substances (EPS) are extracellular metabolites of microorganisms, highly associated with microbial function, adaptation, and growth. The main compounds in EPS have been revealed to be proteins, polysaccharides, nucleic acids, humic substances, lipids, etc. EPS are not only biomass, but also a biogenic material. EPS have high specific surface, abundant functional groups, and excellent degradability. In addition, they are more extensible to the environment than the microbial cells themselves, which exhibits their huge advantages. Therefore, they have been applied in many fields, such as the environment, ecosystem, basic commodities, and medicine. However, the functions of EPS highly depend on the suitable extraction process, as different extraction methods have different effects on their composition, structure, and function. There are many types of EPS extraction methods, in which physical and chemical methods have been widely utilized. This review summarizes the extraction methods and applications of EPS. In addition, it considers some important gaps in current knowledge, and indicates perspectives of EPS for their future study.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"125 ","pages":"79-106"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering of Escherichia coli for efficient production of l-arginine. 高效生产l-精氨酸的大肠杆菌代谢工程。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 DOI: 10.1016/bs.aambs.2022.11.002

Wang Hai-De, Liu Shuai, Wang Bing-Bing, Liu Jie, Xu Jian-Zhong, Zhang Wei-Guo

{"title":"Metabolic engineering of Escherichia coli for efficient production of l-arginine.","authors":"Wang Hai-De, Liu Shuai, Wang Bing-Bing, Liu Jie, Xu Jian-Zhong, Zhang Wei-Guo","doi":"10.1016/bs.aambs.2022.11.002","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.11.002","url":null,"abstract":"As a semi-essential amino acid, l-arginine (l-Arg) plays an important role in food, health care, and medical treatment. At present, the main method of producing l-Arg is the use of microbial fermentation. Therefore, the selection and breeding of high-efficiency microbial strains is the top priority. To continuously improve the l-Arg production performance of the strains, a series of metabolic engineering strategies have been tried to transform the strains. The production of l-Arg by metabolically engineered Corynebacterium glutamicum (C. glutamicum) reached a relatively high level. Escherichia coli (E. coli), as a strain with great potential for l-Arg production, also has a large number of research strategies aimed at screening effective E. coli for producing l-Arg. E. coli also has a number of advantages over C. glutamicum in producing l-Arg. Therefore, it is of great significance to screen out excellent and stable E. coli to produce l-Arg. Here, based on recent research results, we review the metabolic pathways of l-Arg production in E. coli, the research progress of l-Arg production in E. coli, and various regulatory strategies implemented in E. coli.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"122 ","pages":"127-150"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9815493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced imaging techniques: microscopy. 先进的成像技术:显微镜。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 DOI: 10.1016/bs.aambs.2023.01.001

Mona Golmohammadzadeh, Danielle L Sexton, Shweta Parmar, Elitza I Tocheva

{"title":"Advanced imaging techniques: microscopy.","authors":"Mona Golmohammadzadeh, Danielle L Sexton, Shweta Parmar, Elitza I Tocheva","doi":"10.1016/bs.aambs.2023.01.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2023.01.001","url":null,"abstract":"For decades, bacteria were thought of as \"bags\" of enzymes, lacking organelles and significant subcellular structures. This stood in sharp contrast with eukaryotes, where intracellular compartmentalization and the role of large-scale order had been known for a long time. However, the emerging field of Bacterial Cell Biology has established that bacteria are in fact highly organized, with most macromolecular components having specific subcellular locations that can change depending on the cell's physiological state (Barry & Gitai, 2011; Lenz & Søgaard-Andersen, 2011; Thanbichler & Shapiro, 2008). For example, we now know that many processes in bacteria are orchestrated by cytoskeletal proteins, which polymerize into surprisingly diverse superstructures, such as rings, sheets, and tread-milling rods (Pilhofer & Jensen, 2013). These superstructures connect individual proteins, macromolecular assemblies, and even two neighboring cells, to affect essential higher-order processes including cell division, DNA segregation, and motility. Understanding these processes requires resolving the in vivo dynamics and ultrastructure at different functional stages of the cell, at macromolecular resolution and in 3-dimensions (3D). Fluorescence light microscopy (fLM) of tagged proteins is highly valuable for investigating protein localization and dynamics, and the resolution power of transmission electron microscopy (TEM) is required to elucidate the structure of macromolecular complexes in vivo and in vitro. This chapter summarizes the most recent advances in LM and TEM approaches that have revolutionized our knowledge and understanding of the microbial world.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"122 ","pages":"1-25"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9815497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodotorula sp. as a cell factory for production of valuable biomolecules. 红酵母是生产有价值生物分子的细胞工厂。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 DOI: 10.1016/bs.aambs.2023.04.001

Cassamo U Mussagy, Helena F Ribeiro, Jorge F B Pereira

{"title":"Rhodotorula sp. as a cell factory for production of valuable biomolecules.","authors":"Cassamo U Mussagy, Helena F Ribeiro, Jorge F B Pereira","doi":"10.1016/bs.aambs.2023.04.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2023.04.001","url":null,"abstract":"Rhodotorula sp. are well-known for their ability to biosynthesize a diverse range of valuable biomolecules, including carotenoids, lipids, enzymes, and polysaccharides. Despite the high number of studies conducted using Rhodotorula sp. at the laboratory scale, most of these do not address all processual aspects necessary for scaling up these processes for industrial applications. This chapter explores the potential of Rhodotorula sp. as a cell factory for the production of distinct biomolecules, with a particular emphasis on exploring their use from a biorefinery perspective. Through in-depth discussions of the latest research and insights into non-conventional applications, we aim to provide a comprehensive understanding of Rhodotorula sp.'s ability to produce biofuels, bioplastics, pharmaceuticals, and other valuable biochemicals. This book chapter also examines the fundamentals and challenges associated with the optimizing upstream and downstream processing of Rhodotorula sp-based processes. We believe that through this chapter, readers with different levels of expertise will gain insights into strategies for enhancing the sustainability, efficiency, and effectiveness of producing biomolecules using Rhodotorula sp.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"123 ","pages":"133-156"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9861877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Promoters and introns as key drivers for enhanced gene expression in Saccharomyces cerevisiae. 启动子和内含子是酿酒酵母基因表达增强的关键驱动因素。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 Epub Date: 2023-07-27 DOI: 10.1016/bs.aambs.2023.07.002

Marthinus Wessel Myburgh, Kirstie Susan Schwerdtfeger, Rosemary Anne Cripwell, Willem Heber van Zyl, Marinda Viljoen-Bloom

{"title":"Promoters and introns as key drivers for enhanced gene expression in Saccharomyces cerevisiae.","authors":"Marthinus Wessel Myburgh, Kirstie Susan Schwerdtfeger, Rosemary Anne Cripwell, Willem Heber van Zyl, Marinda Viljoen-Bloom","doi":"10.1016/bs.aambs.2023.07.002","DOIUrl":"10.1016/bs.aambs.2023.07.002","url":null,"abstract":"The transcription of genes in the yeast Saccharomyces cerevisiae is governed by multiple layers of regulatory elements and proteins, cooperating to ensure optimum expression of the final protein product based on the cellular requirements. Promoters have always been regarded as the most important determinant of gene transcription, but introns also play a key role in the expression of intron-encoding genes. Some introns can enhance transcription when introduced either promoter-proximal or embedded in the open reading frame of genes. However, the outcome is seldom predictable, with some introns increasing or decreasing transcription depending on the promoter and reporter gene employed. This chapter provides an overview of the general structure and function of promoters and introns and how they may cooperate during transcription to allow intron-mediated enhancement of gene expression. Since S. cerevisiae is a suitable host for recombinant protein production on a commercial level, stronger and more controllable promoters are in high demand. Enhanced gene expression can be achieved via promoter engineering, which may include introns that increase the efficacy of recombinant expression cassettes. Different models for the role of introns in transcription are briefly discussed to show how these intervening sequences can actively interact with the transcription machinery. Furthermore, recent examples of improved protein production via the introduction of promoter-proximal introns are highlighted to showcase the potential value of intron-mediated enhancement of gene expression.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"124 ","pages":"1-29"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10034056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

BioMateriOME: to understand microbe-material interactions within sustainable, living architectures. BioMateriOME:在可持续的、有生命的建筑中理解微生物与物质的相互作用。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 DOI: 10.1016/bs.aambs.2022.11.001

Beatriz Delgado Corrales, Romy Kaiser, Paula Nerlich, Armand Agraviador, Angela Sherry

{"title":"BioMateriOME: to understand microbe-material interactions within sustainable, living architectures.","authors":"Beatriz Delgado Corrales, Romy Kaiser, Paula Nerlich, Armand Agraviador, Angela Sherry","doi":"10.1016/bs.aambs.2022.11.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2022.11.001","url":null,"abstract":"BioMateriOME evolved from a prototyping process which was informed from discussions between a team of designers, architects and microbiologists, when considering constructing with biomaterials or human cohabitation with novel living materials in the built environment. The prototype has two elements (i) BioMateriOME-Public (BMP), an interactive public materials library, and (ii) BioMateriOME-eXperimental (BMX), a replicated materials library for rigorous microbiome experimentation. The prototype was installed into the OME, a unique experimental living house, in order to (1) gain insights into society's perceptions of living materials, and (2) perform a comparative analysis of indoor surface microbiome development on novel biomaterials in contrast to conventional indoor surfaces, respectively. This review summarizes the BioMateriOME prototype and its use as a tool in combining microbiology, design, architecture and social science. The use of microbiology and biological components in the fabrication of biomaterials is provided, together with an appreciation of the microbial communities common to conventional indoor surfaces, and how these communities may change in response to the implementation of living materials in our homes. Societal perceptions of microbiomes and biomaterials, are considered within the framework of healthy architecture. Finally, features of architectural design with microbes in mind are introduced, with the possibility of codifying microbial surveillance into design and construction benchmarks, standards and regulations toward healthier buildings and their occupants.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"122 ","pages":"77-126"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9815494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fundamentals of utilizing microbes in advanced cancer therapeutics: current understanding and potential applications. 在晚期癌症治疗中利用微生物的基本原理:目前的理解和潜在的应用。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 DOI: 10.1016/bs.aambs.2023.03.001

Tashmeen Kaur, Deepika Sharma

{"title":"Fundamentals of utilizing microbes in advanced cancer therapeutics: current understanding and potential applications.","authors":"Tashmeen Kaur, Deepika Sharma","doi":"10.1016/bs.aambs.2023.03.001","DOIUrl":"https://doi.org/10.1016/bs.aambs.2023.03.001","url":null,"abstract":"One of the biggest health related issues in the twenty-first century is cancer. The current therapeutic platforms have not advanced enough to keep up with the number of rising cases. The traditional therapeutic approaches frequently fail to produce the desired outcomes. Therefore, developing new and more potent remedies is crucial. Recently, investigating microorganisms as potential anti-cancer treatments have garnered a lot of attention. Tumor-targeting microorganisms are more versatile at inhibiting cancer than the majority of standard therapies. Bacteria preferentially gather and thrive inside tumors, where they can trigger anti-cancer immune responses. They can be further trained to generate and distribute anticancer drugs based on clinical requirements using straightforward genetic engineering approaches. To improve clinical outcomes, therapeutic strategies utilizing live tumor-targeting bacteria can be used either alone or in combination with existing anticancer treatments. On the other hand, oncolytic viruses that target cancer cells, gene therapy via viral vectors, and viral immunotherapy are other popular areas of biotechnological investigation. Therefore, viruses serve as a unique candidate for anti-tumor therapy. This chapter describes the role of microbes, primarily bacteria and viruses in anti-cancer therapeutics. The various approaches to utilizing microbes in cancer therapy are discussed and examples of microorganisms that are now in use or that are undergoing experimental research are briefly discussed. We further point out the hurdles and the prospects of microbes-based remedies for cancer treatment.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"123 ","pages":"91-131"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10238984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manipulation of fungal cell wall integrity to improve production of fungal natural products. 操纵真菌细胞壁的完整性以提高真菌天然产品的产量。

2区生物学

Advances in applied microbiology Pub Date : 2023-01-01 Epub Date: 2023-09-02 DOI: 10.1016/bs.aambs.2023.07.005

Huiling Liu, Zhengshan Luo, Yijian Rao

{"title":"Manipulation of fungal cell wall integrity to improve production of fungal natural products.","authors":"Huiling Liu, Zhengshan Luo, Yijian Rao","doi":"10.1016/bs.aambs.2023.07.005","DOIUrl":"10.1016/bs.aambs.2023.07.005","url":null,"abstract":"Fungi, as an important industrial microorganism, play an essential role in the production of natural products (NPs) due to their advantages of utilizing cheap raw materials as substrates and strong protein secretion ability. Although many metabolic engineering strategies have been adopted to enhance the biosynthetic pathway of NPs in fungi, the fungal cell wall as a natural barrier tissue is the final and key step that affects the efficiency of NPs synthesis. To date, many important progresses have been achieved in improving the synthesis of NPs by regulating the cell wall structure of fungi. In this review, we systematically summarize and discuss various strategies for modifying the cell wall structure of fungi to improve the synthesis of NPs. At first, the cell wall structure of different types of fungi is systematically described. Then, strategies to disrupt cell wall integrity (CWI) by regulating the synthesis of cell wall polysaccharides and binding proteins are summarized, which have been applied to improve the synthesis of NPs. In addition, we also summarize the studies on the regulation of CWI-related signaling pathway and the addition of exogenous components for regulating CWI to improve the synthesis of NPs. Finally, we propose the current challenges and essential strategies to usher in an era of more extensive manipulation of fungal CWI to improve the production of fungal NPs.","PeriodicalId":7298,"journal":{"name":"Advances in applied microbiology","volume":"125 ","pages":"49-78"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141086534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial mercury transformations: Molecules, functions and organisms. 微生物汞转化:分子、功能和有机体。

2区生物学

Advances in applied microbiology Pub Date : 2022-04-13 DOI: 10.1016/bs.aambs.2022.03.001

Ri-Qing Yu,Tamar Barkay

引用次数: 6