Enfermedades infecciosas y microbiologia clinica (English ed.)最新文献

{"title":"Impact of automated nucleic acid extraction platforms on plasma Cytomegalovirus DNA loads quantitated by real-time PCR normalized to the 1st WHO international standard","authors":"Ángela Sánchez-Simarro ,&nbsp;Eliseo Albert ,&nbsp;Paula Michelena ,&nbsp;Estela Giménez ,&nbsp;David Navarro","doi":"10.1016/j.eimce.2024.07.007","DOIUrl":"10.1016/j.eimce.2024.07.007","url":null,"abstract":"<div><h3>Introduction</h3><div>The extent to which commercially available nucleic acid extraction platforms impact the magnitude of Cytomegalovirus (CMV) DNA loads measured in plasma specimens by 1st WHO standard-normalized real-time PCR assays is uncertain.</div></div><div><h3>Methods</h3><div>This retrospective study compares the performance of Abbott m2000sp, Qiagen QIAsymphony SP, and KingFisher Flex platforms using plasma samples from allogeneic hematopoietic stem cell transplant recipients and plasma spiked with the CMV AD169 strain. The Abbott RealTi<em>m</em>e CMV PCR assay was used for CMV DNA quantitation.</div></div><div><h3>Results</h3><div>Maximum differences in CMV DNA loads quantified in plasma from 11 allo-HSCT and spiked plasma over a wide range of viral DNA concentrations (2.0–4.0 log₁₀ IU/ml) were ≤0.5 log₁₀ IU/ml.</div></div><div><h3>Conclusions</h3><div>The CMV DNA extraction efficiency of the platforms evaluated varies. The impact of these variations on CMV DNA loads quantified in plasma may not be clinically relevant.</div></div>","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"43 1","pages":"Pages 28-31"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Indirect impact of PCV10 children vaccination on the serotype distribution and antimicrobial resistance of Streptococcus pneumoniae causing invasive disease in adults over 50 in Colombia, 2005–2019: Observational analysis","authors":"Carolina Duarte ,&nbsp;Clara Inés Agudelo ,&nbsp;Carlos Castañeda-Orjuela ,&nbsp;Jaime Moreno ,&nbsp;Olga Marina Sanabria ,&nbsp;Adriana Bautista ,&nbsp;Elizabeth Castañeda","doi":"10.1016/j.eimce.2023.07.008","DOIUrl":"10.1016/j.eimce.2023.07.008","url":null,"abstract":"<div><h3>Introduction</h3><div><span>The introduction of pneumococcal conjugate vaccine (PCV) into childhood vaccination programmes has reduced the prevalence of vaccine serotypes (VTs) that cause invasive pneumococcal disease (IPD) in children. In the elderly population, an impact has also been seen through indirect protection (herd effect). The aim of this study was to estimate the changes in serotype distribution and antimicrobial susceptibility of </span><span><span>Streptococcus pneumoniae</span></span> isolates recovered from adult IPD and to evaluate the indirect effect of immunization with PCV10 based on laboratory records by analyzing the period from 2005 to 2019 for six years before and eight years after the universal PCV10 administration to Colombian children.</div></div><div><h3>Methods</h3><div>A total of 2204 <span><em>S. pneumoniae</em></span> isolates from adults (≥50 years) with IPD were analyzed. The analysis examined the percentage changes in proportions (prevalence) and percentage variations in population rates (annual reported rates – ARR) of VTs between the pre-PCV10 (2005–2009) and post-PCV10 (2015–2019) periods.</div></div><div><h3>Results</h3><div>The findings were (1) evidence of a significant percentage decrease of pneumococcal VT10 causing IPD in adults (50% pre-PCV10 and 16% post-PCV10); (2) significant increase of serotype 19A (from 1.6% to 14.8%) and less important increase of serotype 3 (from 10.5% to 14.5%) and non-vaccine serotypes (NVT) (from 21.4% to 38.4%) non-significant; and (3) meningitis and non-meningitis multidrug resistant isolates associated with serotype 19A. An improvement in the surveillance system is associated with the immunization of children, as noted by the increased ARRs across the analysis period.</div></div><div><h3>Conclusions</h3><div>Our results show the indirect impact of PCV10 vaccination in children on the VT10 distribution and antimicrobial resistance of <em>S. pneumoniae</em> causing IPD in Colombian adults over 50 when comparing the pre-PCV10 (2005–2009) and post-PCV10 (2015–2019) periods.</div></div>","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"43 1","pages":"Pages 3-9"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72016341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of carbapenemases in Enterobacterales susceptible in vitro to meropenem","authors":"Luana Silva Dornelles ,&nbsp;Mariana Preussler Mott ,&nbsp;Gabriela da Silva Collar ,&nbsp;Luciana Giordani ,&nbsp;Rodrigo Minuto Paiva ,&nbsp;Larissa Lutz","doi":"10.1016/j.eimce.2024.07.008","DOIUrl":"10.1016/j.eimce.2024.07.008","url":null,"abstract":"<div><h3>Introduction</h3><div>Carbapenemase-producing Enterobacterales (CPE) is a global threat. We evaluate the prevalence of CPE among isolates categorized as meropenem-susceptible, but that meet the European Committee on Antimicrobial Susceptibility Testing (EUCAST) screening cut-off values for carbapenemase detection, and analyze the susceptibility of these isolates to new available drugs.</div></div><div><h3>Methods</h3><div>We analyzed 257 isolates from patients hospitalized in a tertiary hospital in Brazil, from July 2022 to April 2023. Only isolates that met the screening cut-off values established by EUCAST for detection of carbapenemases were analyzed (i.e. meropenem inhibition zones of 25–27<!--> <!-->mm by disk diffusion). The detection of carbapenemases was performed by immnunochromatographic testing and confirmed by high-resolution melting-PCR (HRM-qPCR).</div></div><div><h3>Results</h3><div>We identified 12 (4.7%) CPE including 7 KPC, 4 NDM, and 1 OXA-48-like. The isolates were susceptible to ceftazidime–avibactam (72.7%), meropenem–vaborbactam (100%), imipenem–relebactam (63.6%) and ceftolozane–tazobactam (36.4%).</div></div><div><h3>Conclusion</h3><div>We highlight the importance of tracking carbapenemases for epidemiological control and therapeutic guidance.</div></div>","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"43 1","pages":"Pages 32-35"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should we implant a pacemaker in a young patient with high-grade atrioventricular block? The importance of clinical suspicion in Lyme carditis","authors":"Ester Minguez de la Guía,&nbsp;Macarena López Vázquez,&nbsp;Pablo Miguel Valentín García,&nbsp;Miguel José Corbi Pascual","doi":"10.1016/j.eimce.2024.10.003","DOIUrl":"10.1016/j.eimce.2024.10.003","url":null,"abstract":"","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"43 1","pages":"Pages 51-52"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolving SARS-CoV-2 severity among hospital and university affiliates in Spain and Greater Boston","authors":"Fares Amer ,&nbsp;Fan-Yun Lan ,&nbsp;Mario Gil-Conesa ,&nbsp;Amalia Sidossis ,&nbsp;Daniel Bruque ,&nbsp;Eirini Iliaki ,&nbsp;Jane Buley ,&nbsp;Neetha Nathan ,&nbsp;Lou Ann Bruno-Murtha ,&nbsp;Silvia Carlos ,&nbsp;Stefanos N. Kales ,&nbsp;Alejandro Fernandez-Montero","doi":"10.1016/j.eimce.2023.12.004","DOIUrl":"10.1016/j.eimce.2023.12.004","url":null,"abstract":"<div><h3>Introduction</h3><div>The COVID-19 pandemic caused by the SARS-CoV-2 virus greatly affected healthcare workers and healthcare systems. It also challenged schools and universities worldwide negatively affecting in-person education. We conducted this study is to assess the evolution of SARs-CoV-2 virulence over the course of the pandemic.</div></div><div><h3>Methods</h3><div>A combined cohort of University students in Spain and HCWs from the two hospitals in Spain, and one healthcare system in the Greater Boston area was followed prospectively from March 8th, 2020, to January 31st, 2022 for diagnosis with COVID-19 by PCR testing and related sequelae. Follow-up time was divided into four periods according to distinct waves of infection during the pandemic. Severity of COVID-19 was measured by case-hospitalization rate. Descriptive statistics and multivariable-adjusted statistics using the Poisson mixed-effects regression model were applied. As a sensitivity analysis, information on SARS-CoV-2 RNA in wastewater and COVID-19 deaths through May 30, 2023 from the Boston area was collected.</div></div><div><h3>Results</h3><div>For the last two periods of the study (January 1st to December 15th, 2021 and December 16th, 2021 to January 31st, 2022) and relative to the first period (March 8th to May 31st, 2020), the incidence rate ratios (IRRs) of hospitalization were 0.08 (95% CI, 0.03–0.17) and 0.03 (95% CI, 0.01–0.15), respectively. In addition, a relative risk 0.012 CI95% (0.012–0.012) was observed when comparing COVID-19 mortality versus SARS-CoV-2 RNA copies/mL in Boston-area wastewater over the period (16th December 2021 to 30th May 2023) and relative to the first period.</div></div><div><h3>Conclusions</h3><div>The severity of COVID-19 and immunity of our populations evolved over time, resulting in a decrease in case severity. We found the case-hospitalization rate decreased more than 90% in our cohort despite an increase in incidence.</div></div>","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"43 1","pages":"Pages 17-22"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cost analysis associated with intramuscular versus oral administration of antiretroviral therapy in the management of human immunodeficiency virus infection.","authors":"Vicente Estrada, Juan Emilio Losa, Ramón Morillo-Verdugo, Montserrat Pérez-Encinas, Jesús Santos, Antonio Castro, María Presa González, Laura Salinas-Ortega","doi":"10.1016/j.eimce.2024.12.003","DOIUrl":"https://doi.org/10.1016/j.eimce.2024.12.003","url":null,"abstract":"<p><strong>Objetive: </strong>To identify and analyze the resources and costs associated with the administration of intramuscular antiretroviral therapy (ART) cabotegravir+rilpivirine (CAB+RPV) compared to oral ART in the management of Human Immunodeficiency Virus Type 1 (HIV-1) infection in Spain.</p><p><strong>Methods: </strong>An economic model was developed to identify resources and analyze costs from the perspective of the National Health System (NHS) and societal, associated with the administration of intramuscular ART (CAB+RPV) compared to oral ART over a two-year time horizon. Costs included treatment change monitoring, pharmaceutical dispensation, administration, management of adverse events to injection-site reactions (AEs-ISR), travel to the hospital, telepharmacy service, and lost work productivity. Unit costs (€, 2023) were obtained from the literature. Sensitivity analyses were conducted to evaluate the robustness of the model.</p><p><strong>Results: </strong>Intramuscular ART compared to oral ART was associated with an increase in costs of €673.16/patient over two years from the perspective of the NHS, and €719.59/patient from the social perspective. Intramuscular ART would generate increased costs for dispensation (+€97.75), administration (+€394.55), monitoring (+€288.74), management of AEs-ISR (+€6.46), travel (+€8.36), and lost work productivity (+€38.07), compared to oral ART administration.</p><p><strong>Conclusion: </strong>Treating HIV-1 with intramuscular CAB+RPV leads to increased resource consumption and costs, compared to oral ART.</p>","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142911267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Papular lesions and pancytopenia in a patient with severe immunosuppression due to HIV.","authors":"Laia Arbós Junyent, Antonio Casabella Pernas, Granada Olga Perea Duran, Marta Navarro Vilasaró","doi":"10.1016/j.eimce.2024.12.005","DOIUrl":"https://doi.org/10.1016/j.eimce.2024.12.005","url":null,"abstract":"","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proptosis and head circumference enlargement during puerperium.","authors":"Lucía Guillén Zafra, Pedro Manuel García-Ceberino, Diana Alejandra Pantoja Pachajoa, Juan José Pomares Pomares","doi":"10.1016/j.eimce.2024.12.006","DOIUrl":"https://doi.org/10.1016/j.eimce.2024.12.006","url":null,"abstract":"","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tolerance of Atovacuona/proguanil in off-label indication in children","authors":"Lorea Vicente Elcano ,&nbsp;Cecilia Muruzábal Pino ,&nbsp;Cristina Calvo ,&nbsp;Milagros García López Hortelano","doi":"10.1016/j.eimce.2024.06.009","DOIUrl":"10.1016/j.eimce.2024.06.009","url":null,"abstract":"","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"42 10","pages":"Pages 605-606"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistent constipation. A case report","authors":"J.G. Sánchez Cano ,&nbsp;D. Gayoso Cantero ,&nbsp;L. Moreno Núñez","doi":"10.1016/j.eimce.2024.05.001","DOIUrl":"10.1016/j.eimce.2024.05.001","url":null,"abstract":"","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"42 10","pages":"Pages 597-598"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}