Development & reproduction最新文献_第8页

Ethics and Responsibility of Scientific Researchers in a Pandemic Era. 大流行时代科研人员的伦理与责任

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.321

Kyung-Hee Lee

{"title":"Ethics and Responsibility of Scientific Researchers in a Pandemic Era.","authors":"Kyung-Hee Lee","doi":"10.12717/DR.2021.25.4.321","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.321","url":null,"abstract":"The purpose of this paper is to critique the links between science and scientific researchers, politics and capital surrounding vaccines and vaccination in a pandemic era. It also introduces standards for adapting ethical guidelines for research under public health emergencies to specific circumstances and contexts. It also introduces ethical standards to be applied to scientific research. that is, scientific relevance, social value, cooperative partnerships, reasonable risk-benefit costs, fair and voluntary participation, independent review, and equal moral respect for participants and affected communities. It also outlines the COVID-19 (coronavirus disease 2019) pandemic-consent and other research procedure modifications proposed by Oregon Health and Science University (OHSU). We are on the lookout for powerful capital and hegemonic groups inseparable from politics and important decision makers in the pandemic era - that is, scientists, supporters, and scientific civic groups. It underscores the need for an independent and formal scientific advisory body with the right balance between science and politics.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"321-326"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/05/dr-25-4-321.PMC8807136.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39781496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of Miniaturized Ovaries by In Vitro Culture from Mouse Gonads. 小鼠性腺体外培养制备小型卵巢的研究。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2020.25.3.173

Si Won Jang, Hyun Woo Choi

引用次数: 0

Maternal Exposure to Bisphenol A Impacts on Fecundity in F1 and F2 Generations in Drosophila melanogaster. 母体暴露于双酚A对黑腹果蝇F1和F2代繁殖能力的影响。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.193

Sohee Kim, Kyong-Hwa Kang, Hyongjong Koh

引用次数: 0

Reproduction and Maturation of Sea Bass, Lateolabrax japonicus, after Transportation from Net-Cages to Indoor Tanks. 日本鲈鱼从网箱转移到室内池后的繁殖和成熟。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.157

Duk-Young Kang, Hyo-Chan Kim, Jae Hyun Im

{"title":"Reproduction and Maturation of Sea Bass, Lateolabrax japonicus, after Transportation from Net-Cages to Indoor Tanks.","authors":"Duk-Young Kang, Hyo-Chan Kim, Jae Hyun Im","doi":"10.12717/DR.2021.25.3.157","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.157","url":null,"abstract":"To determine whether the reproductive processes of sea bass, Lateolabrax japonicus, proceed normally after transportation from an outdoor net-cage into indoor tanks, we examined changes in the gonadosomatic index (GSI), histological gonadal tissue, and plasma levels of sex hormones (testosterone and estradiol-17ß) during their annual reproductive cycle. We also measured maturation and spawning across two sea water salinity levels (full and low salinity). Fecundity was estimated by the relationship between egg number and body size in female sea bass. Monthly changes in the GSI, histological gonadal tissues, and oocyte size showed both male and female sea bass reach final maturation in January and February, respectively, indicating that the spermiation of males occurs earlier than the spawning of females. The histological results indicated that the sea bass is a multiple spawner, similar to many marine teleosts, exhibiting group-synchronous oocyte development. Female maturation and spawning were enhanced in lower salinity seawater (29.6-31.0 psu) compared to that of normal salinity (34.5-35.1 psu). These results confirm that sea bass reproduction can occur successfully in captivity and imply that fertilized eggs can be collected from February to March. Additionally, our results show that lower salinity enhances oocyte maturation and spawning of female sea bass.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"157-171"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/61/dr-25-3-157.PMC8670778.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39636608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gonadal Abnormality and Intersexuality of Oplegnathus fasciatus (Teleostei: Oplegnathidae) Collected from the Southern Coast of Korea: A Case Report. 韩国南部海岸采集的筋膜钩鱼性腺异常和雌雄间性1例。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.123

Ji Sung Choi, So Ryung Shin, Hyeon Jin Kim, Hyejin Kim, Yeonghye Kim, Jung Sick Lee

{"title":"Gonadal Abnormality and Intersexuality of Oplegnathus fasciatus (Teleostei: Oplegnathidae) Collected from the Southern Coast of Korea: A Case Report.","authors":"Ji Sung Choi, So Ryung Shin, Hyeon Jin Kim, Hyejin Kim, Yeonghye Kim, Jung Sick Lee","doi":"10.12717/DR.2021.25.3.123","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.123","url":null,"abstract":"We report on the gonadal structure abnormality and intersexuality found from a survey regarding the reproductive potential of the rock bream, Oplegnathus fasciatus, along the southern coast of Korea. In total, 448 samples were collected for histological analysis (total length 27.5±6.8 cm; total weight 522.9±385.6 g). The sex ratio (F:M) was 1:0.46 (n=307:141), with 68.5% being females. The frequency of oocyte atresia and ovarian neoplasm in the females was 85.3% and 21.5%, respectively, and the frequency of testicular lobule and cyst deformation and testicular neoplasm in the males was 73.1% and 37.6%, respectively. The scale formation in the ovary and testis was approximately 2.0% for both sexes. The intersexuality was 42.2%, with the intersexuality among females being 49.5%, which was significantly higher than that of males (26.2%). Such findings indicate a negative impact on the reproductive output of the rock bream along the southern coast of Korea; however, a more detailed study is required to ascertain accurate causes.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"123-131"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a7/6f/dr-25-3-123.PMC8670776.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39621055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reproductive Cycle of Blue-Striped Angelfish, Chaetodontoplus septentrionalis in Jeju Coastal Waters. 济州岛近海蓝条纹天使鱼的繁殖周期。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.145

Dae-Jong Kim, Chi-Hoon Lee, Young-Don Lee

{"title":"Reproductive Cycle of Blue-Striped Angelfish, Chaetodontoplus septentrionalis in Jeju Coastal Waters.","authors":"Dae-Jong Kim, Chi-Hoon Lee, Young-Don Lee","doi":"10.12717/DR.2021.25.3.145","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.145","url":null,"abstract":"Reproductive cycle of the blue-striped angelfish, Chaetodontoplus septentrionalis were histologically investigated. Fish were monthly collected in the coastal waters of Munseom, Seogwipo, Jeju-Island from February to December 2018. The gonadosomatic index (GSI) increased from May and maintained high values in August. The reproductive cycle of female fish can be classified by the characteristics observed during gonadal development as followed: growing stage (November to June), early mature stage (May to June), mature and spawning stage (June to September), and degenerative and recovery stage (September to December). In the male, testicular development period was similar to that of ovarian development period, but mature and spawning period was one month longer from June to October. Fecundity of mature female ranged from 4,601 to 22,840 and was correlated positively with total length and body weight. The histological analysis of gonadal development indicated that the C. septentrionalis was summer-spawning type and is considered a multiple spawner during spawning season.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"145-155"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/6b/dr-25-3-145.PMC8670773.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39621057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Variations within and between Blue Crab (Portunus trituberculatus) Groups. 蓝蟹（Portunus trituberculatus）群体内部和群体之间的遗传变异。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.185

Young-Jae Song, Jong-Man Yoon

{"title":"Genetic Variations within and between Blue Crab (Portunus trituberculatus) Groups.","authors":"Young-Jae Song, Jong-Man Yoon","doi":"10.12717/DR.2021.25.3.185","DOIUrl":"10.12717/DR.2021.25.3.185","url":null,"abstract":"Abstract The five oligonucleotide primers (oligo-primers) turned out a total of 335 fragments (FMs) (52.9%) in the blue crab (Portunus trituberculatus) group alpha and 298 FMs (47.1%) in the crab group beta, with the FM scales range varying from 100 bp to 2,000 bp. The highest band-sharing (BS) value (0.907) was found between individual’s no. 19 and no. 20 within the blue crab group beta. Parties in the blue crab group beta (0.601±0.017) had higher BS rates than did parties from the crab group alpha (0.563±0.017) (p<0.05). The polar dendrogram got by the five oligo-primers points out two genetic extents: bundle I (BLUECRAB 01, 03, 04, 05, 06, 08, and 10) and bundle II (BLUECRAB 02, 07, 09. 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The OPD-01 primer revealed 22 loci shared by all the examples of the as FMs of 1,000 bp. The oligo-primer OPA-05 made unique loci shared to each group (ULSEG), almost 400 bp and 500 bp, individually, in blue crab group beta. The remaining oligo-primers did not reveal any loci shared by the two crab groups (LSTG). The average number of ULSEG was diverse and 1.6-fold higher in the crab group beta than in the crab group alpha.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"185-192"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/87/dr-25-3-185.PMC8670772.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39636610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constitutive Activating Eel Luteinizing Hormone Receptors Induce Constitutively Signal Transduction and Inactivating Mutants Impair Biological Activity. 组成激活黄体生成素受体诱导组成信号转导，灭活突变体损害生物活性。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.133

Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min

{"title":"Constitutive Activating Eel Luteinizing Hormone Receptors Induce Constitutively Signal Transduction and Inactivating Mutants Impair Biological Activity.","authors":"Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min","doi":"10.12717/DR.2021.25.3.133","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.133","url":null,"abstract":"In contrast to the human lutropin receptor (hLHR) and rat LHR (rLHR), very few naturally occurring mutants in other mammalian species have been identified. The present study aimed to delineate the mechanism of signal transduction by three constitutively activating mutants (designated M410T, L469R, and D590Y) and two inactivating mutants (D383N and Y546F) of the eel LHR, known to be naturally occurring in human LHR transmembrane domains. The mutants were constructed and measured cyclic adenosine monophosphate (cAMP) accumulation via homogeneous time-resolved fluorescence assays in Chinese hamster ovary (CHO)-K1 cells. The activating mutant cells expressing eel LHR-M410T, L469R, and D590Y exhibited a 4.0-, 19.1-, and 7.8-fold increase in basal cAMP response without agonist treatment, respectively. However, inactivating mutant cells expressing D417N and Y558F did not completely impaired signal transduction. Specifically, signal transduction in the cells expressing activating mutant L469R was not occurred with a further ligand stimulation, showing that the maximal response exhibited approximately 53% of those of wild type receptor. Our results suggested that the constitutively activating mutants of the eel LHR consistently occurred without agonist treatment. These results provide important information of LHR function in fish and regulation with regard to mutations of highly conserved amino acids in glycoprotein hormone receptors.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"133-143"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/5c/dr-25-3-133.PMC8670774.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39621056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Antitumor Effect of Metformin in Combination with Binimetinib on Melanoma Cells. 二甲双胍联合比尼美替尼对黑色素瘤细胞的抗肿瘤作用。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.93

Eunsung Lee, Yongjae Kwon, Jiwon Kim, Deokbae Park, Youngki Lee

{"title":"Antitumor Effect of Metformin in Combination with Binimetinib on Melanoma Cells.","authors":"Eunsung Lee, Yongjae Kwon, Jiwon Kim, Deokbae Park, Youngki Lee","doi":"10.12717/DR.2021.25.2.93","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.93","url":null,"abstract":"Cutaneous melanoma is a fatal disease for patients with distant metastasis. Metformin is the most widely used anti-diabetic drug, and proved to suppress cell proliferation and metastasis in diverse cancers including melanoma. We previously reported that MEK inhibitor trametinib increases the expression of epithelial-mesenchymal transition (EMT) regulators and melanoma cell motility, which are suppressed by addition of metformin in A375 melanoma cells. To confirm our findings further, we first evaluated the effect of metformin in combination with another MEK inhibitor binimetinib on cell viability in G361 melanoma cells. We then investigated whether binimetinib affects the expression of EMT regulators and cell motility. We finally monitored the effect of metformin on binimetinib-induced cell migration. Cell viability assay showed that combination index (CI) value at ED50 is 0.80, suggesting synergy for the combination of metformin with binimetinib. Our results also revealed that binimetinib increased the expression of EMT regulators such as integrin αV, fibronectin and slug, which correlate well with the enhanced cell migration in wound healing assay. Metformin, on the contrary, suppressed the expression of sparc, integrin αV, fibronectin and N-cadherin with the reduced cell motility. The combination treatment showed that metformin counteracts the binimetinib-induced increase of cell motility. Overall, these results suggest that metformin with binimetinib might be useful as a potential therapeutic adjuvant against cell survival and metastatic activity in melanoma patients.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"93-104"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/79/dr-25-2-93.PMC8328479.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Geographical Genetic Differences of Arkshell Populations in Korea. 韩国黑壳居群地理遗传差异分析。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.105

Jong-Man Yoon

{"title":"Analysis of Geographical Genetic Differences of Arkshell Populations in Korea.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2021.25.2.105","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.105","url":null,"abstract":"A PCR-founded genetic analysis aim and principle was used to foster a hierarchical polar dendrogram of the Euclidean genetic distances (GDs) for two arkshell populations, Scapharca broughtonii (YEOSU, Yeosu population and JINHAE, Jinhae population). Five oligonucleotides primers were make use of to craft 354 and 390 scorable bands in the Yeosu and Jinhae populations, respectively, outspreading in DNA fragment size from 100 bp to 1,600 bp. The bandsharing (BS) results disclosed that the Jinhae population had a higher average BS value (0.700) than that for the Yeosu population (0.692). The GD between individuals supported an adjacent association in grouping II (JINHAE 12 - JINHAE 22). The observation of a noteworthy GD between the two Scapharca populations verified that this PCR-generated technique could be a profitable attempt for within- and between-population-grounded biological DNA scrutiny. The potential of PCR inquiry will be favorable in the selection of individuals and/or populations for several reproductive- and/or quarantine-connected characters in aquafarming manufacture.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"105-111"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/eb/dr-25-2-105.PMC8328478.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1