Development & reproduction最新文献_第8页

Genetic Distances for Intra- and Between-Group of Scapharca subcrenata from Yeosu of the Korea. 韩国丽水亚鲫组内和组间的遗传距离。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.305

Jong-Man Yoon

{"title":"Genetic Distances for Intra- and Between-Group of Scapharca subcrenata from Yeosu of the Korea.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2021.25.4.305","DOIUrl":"10.12717/DR.2021.25.4.305","url":null,"abstract":"The oligonucleotides polymers (ON-polymers) were used producing a total of 110 loci unique to each clam population (LUECP) in group one and 132 in group two, respectively, varying in amount of DNA fragments (FRs) from greater than near 50 to a smaller quantity than 1,050 bp. The larger FR amounts (>1,050 bp) are not noticed in the two Scapharca subcrenata groups. The ON-polymer OPD-01 produced 33 LUECP, which were defining each group, almost 300 bp, 450 bp, and 500 bp, in the group one. The OPD-15 recognized 22 loci shared by the two clam populations (Loci shared by the two clam populations, LSTCP), a variety of FRs of sizes 300 bp that were equivalent in all specimens. The mean number of LUECP was varied and 1.2-fold greater in the shellfish group two than in the group one. Respecting mean bandsharing (BS) grade outcomes, entities in the shellfish group one (0.779±0.011) had a little higher BS grades than did entities from the group two (0.756±0.009) (p<0.05). The entities of the shellfish group one are not tightly gathered with other entities of the group two. The genetic distance (GD) (0.422) of this invertebrate (SUBCRENATA 02 and 01) is 7.41-fold hereditarily distinct to the GD (0.057) of the other invertebrate (SUBCRENATA 22 and 19). The polar dendrogram (PDG) procured by the five ON-polymers underlines two characteristic groups.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"305-311"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/43/dr-25-4-305.PMC8807124.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39781494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-Surface Loss of Constitutive Activating and Inactivating Mutants of Eel Luteinizing Hormone Receptors. 鳗鱼黄体生成素受体组成型激活和失活突变体的细胞表面损失。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.225

Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min

{"title":"Cell-Surface Loss of Constitutive Activating and Inactivating Mutants of Eel Luteinizing Hormone Receptors.","authors":"Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min","doi":"10.12717/DR.2021.25.4.225","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.225","url":null,"abstract":"The present study aimed to investigate the mechanism of cell surface receptor loss by two constitutively activating mutants (designated L469R, and D590Y) and two inactivating mutants (D417N and Y558F) of the luteinizing hormone receptor (LHR) in the Japanese eel Anguilla japonica, known to naturally occur in human LHR transmembrane domains. We investigated cell surface receptor loss using an enzyme-linked immunosorbent assay in HEK 293 cells. The expression level of wild-type eel LHR was considered to be 100%, and the expression levels of L469R and D417N were 97% and 101%, respectively, whereas the expression levels of D590Y and Y558F slightly increased to approximately 110% and 106%, respectively. The constitutively activating mutants L469R and D590Y exhibited a decrease in cell surface loss in a manner similar to that of wild-type eel LHR. The rates of loss of cell surface agonist-receptor complexes were observed to be very rapid (2.6-6.2 min) in both the wild-type eel LHR and activating mutants. However, cell surface receptor loss in the cells expressing inactivating mutants D417N and Y558F was slightly observed in the cells expressing inactivating mutants D417N and Y558F, despite treatment with a high concentration of agonist. These results provide important information on LHR function in fish and the regulation of mutations of highly conserved amino acids in glycoprotein hormone receptors.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"225-234"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/27/dr-25-4-225.PMC8807125.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ovarian Development of Conger Eel in Korea, Conger myriaster, in Captivity. 韩国长鳗鲡卵巢发育，长鳗鲡，人工饲养。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.269

Se-Un Ki, Chung-Kug Park, Kyoung-Woo Lee, Kyoung-Sik Lee, Joon-Taek Park, Won-Kyo Lee

{"title":"Ovarian Development of Conger Eel in Korea, Conger myriaster, in Captivity.","authors":"Se-Un Ki, Chung-Kug Park, Kyoung-Woo Lee, Kyoung-Sik Lee, Joon-Taek Park, Won-Kyo Lee","doi":"10.12717/DR.2021.25.4.269","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.269","url":null,"abstract":"Effects of water temperature and hormones on ovarian development of conger eel in Korea were investigated. Ovarian development was analyzed by measuring gonadosomatic index (GSI) and oocyte diameter with histological methods. At rearing water temperatures of 12°C, 14°C, and 16°C, GSI value increased from 3.66 at the start of the experiment to 7.44, 8.82, and 7.34 at the end of the experiment, respectively. At rearing water temperatures of 12°C, 14°C, and 16°C, egg diameter increased from 245.11-300.25 µm at the start of the experiment to 377.62-480.27 µm, 396.72-498.54 µm, and 382.29-475.69 µm at the end of the experiment, respectively. Follicular oocyte development revealed that primary yolk globule stage observed from January to March. It entered to secondary yolk globule stage in April and remained at the same stage until July. As a result of examining effects of three hormones (human chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analogue (LHRHa), and salmon pituitary extraction (SPE) on ovarian development, HCG was found to be the most effective one. The progress from diapause of the secondary yolk globule stage to migratory nucleus stage of oocytes could be induced by treating fish with HCG at 1,000 IU/kg. The effect of hormone treatment on ovarian development of conger eel in Korea was the most effective at water temperature of 14°C.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"269-277"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f5/e7/dr-25-4-269.PMC8807132.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethics and Responsibility of Scientific Researchers in a Pandemic Era. 大流行时代科研人员的伦理与责任

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.321

Kyung-Hee Lee

{"title":"Ethics and Responsibility of Scientific Researchers in a Pandemic Era.","authors":"Kyung-Hee Lee","doi":"10.12717/DR.2021.25.4.321","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.321","url":null,"abstract":"The purpose of this paper is to critique the links between science and scientific researchers, politics and capital surrounding vaccines and vaccination in a pandemic era. It also introduces standards for adapting ethical guidelines for research under public health emergencies to specific circumstances and contexts. It also introduces ethical standards to be applied to scientific research. that is, scientific relevance, social value, cooperative partnerships, reasonable risk-benefit costs, fair and voluntary participation, independent review, and equal moral respect for participants and affected communities. It also outlines the COVID-19 (coronavirus disease 2019) pandemic-consent and other research procedure modifications proposed by Oregon Health and Science University (OHSU). We are on the lookout for powerful capital and hegemonic groups inseparable from politics and important decision makers in the pandemic era - that is, scientists, supporters, and scientific civic groups. It underscores the need for an independent and formal scientific advisory body with the right balance between science and politics.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"321-326"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/05/dr-25-4-321.PMC8807136.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39781496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor. 马黄体生成素/CG (eLH/CG)受体表达细胞中绒毛膜促性腺激素(eCG)糖基化位点的特异性生物活性

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.199

Munkhzaya Byambaragchaa, Seung-Hee Choi, Hyo-Eun Joo, Sang-Gwon Kim, Yean-Ji Kim, Gyeong-Eun Park, Myung-Hwa Kang, Kwan-Sik Min

{"title":"Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.","authors":"Munkhzaya Byambaragchaa, Seung-Hee Choi, Hyo-Eun Joo, Sang-Gwon Kim, Yean-Ji Kim, Gyeong-Eun Park, Myung-Hwa Kang, Kwan-Sik Min","doi":"10.12717/DR.2021.25.4.199","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.199","url":null,"abstract":"Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species. However, in equidaes, eCG exhibits only LH-like activity. To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant. We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells. We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased. The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG. The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells). Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR. These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"199-211"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/48/dr-25-4-199.PMC8807129.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Generation of Miniaturized Ovaries by In Vitro Culture from Mouse Gonads. 小鼠性腺体外培养制备小型卵巢的研究。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2020.25.3.173

Si Won Jang, Hyun Woo Choi

引用次数: 0

Maternal Exposure to Bisphenol A Impacts on Fecundity in F1 and F2 Generations in Drosophila melanogaster. 母体暴露于双酚A对黑腹果蝇F1和F2代繁殖能力的影响。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.193

Sohee Kim, Kyong-Hwa Kang, Hyongjong Koh

引用次数: 0

Reproduction and Maturation of Sea Bass, Lateolabrax japonicus, after Transportation from Net-Cages to Indoor Tanks. 日本鲈鱼从网箱转移到室内池后的繁殖和成熟。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.157

Duk-Young Kang, Hyo-Chan Kim, Jae Hyun Im

{"title":"Reproduction and Maturation of Sea Bass, Lateolabrax japonicus, after Transportation from Net-Cages to Indoor Tanks.","authors":"Duk-Young Kang, Hyo-Chan Kim, Jae Hyun Im","doi":"10.12717/DR.2021.25.3.157","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.157","url":null,"abstract":"To determine whether the reproductive processes of sea bass, Lateolabrax japonicus, proceed normally after transportation from an outdoor net-cage into indoor tanks, we examined changes in the gonadosomatic index (GSI), histological gonadal tissue, and plasma levels of sex hormones (testosterone and estradiol-17ß) during their annual reproductive cycle. We also measured maturation and spawning across two sea water salinity levels (full and low salinity). Fecundity was estimated by the relationship between egg number and body size in female sea bass. Monthly changes in the GSI, histological gonadal tissues, and oocyte size showed both male and female sea bass reach final maturation in January and February, respectively, indicating that the spermiation of males occurs earlier than the spawning of females. The histological results indicated that the sea bass is a multiple spawner, similar to many marine teleosts, exhibiting group-synchronous oocyte development. Female maturation and spawning were enhanced in lower salinity seawater (29.6-31.0 psu) compared to that of normal salinity (34.5-35.1 psu). These results confirm that sea bass reproduction can occur successfully in captivity and imply that fertilized eggs can be collected from February to March. Additionally, our results show that lower salinity enhances oocyte maturation and spawning of female sea bass.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"157-171"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/61/dr-25-3-157.PMC8670778.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39636608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gonadal Abnormality and Intersexuality of Oplegnathus fasciatus (Teleostei: Oplegnathidae) Collected from the Southern Coast of Korea: A Case Report. 韩国南部海岸采集的筋膜钩鱼性腺异常和雌雄间性1例。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.123

Ji Sung Choi, So Ryung Shin, Hyeon Jin Kim, Hyejin Kim, Yeonghye Kim, Jung Sick Lee

{"title":"Gonadal Abnormality and Intersexuality of Oplegnathus fasciatus (Teleostei: Oplegnathidae) Collected from the Southern Coast of Korea: A Case Report.","authors":"Ji Sung Choi, So Ryung Shin, Hyeon Jin Kim, Hyejin Kim, Yeonghye Kim, Jung Sick Lee","doi":"10.12717/DR.2021.25.3.123","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.123","url":null,"abstract":"We report on the gonadal structure abnormality and intersexuality found from a survey regarding the reproductive potential of the rock bream, Oplegnathus fasciatus, along the southern coast of Korea. In total, 448 samples were collected for histological analysis (total length 27.5±6.8 cm; total weight 522.9±385.6 g). The sex ratio (F:M) was 1:0.46 (n=307:141), with 68.5% being females. The frequency of oocyte atresia and ovarian neoplasm in the females was 85.3% and 21.5%, respectively, and the frequency of testicular lobule and cyst deformation and testicular neoplasm in the males was 73.1% and 37.6%, respectively. The scale formation in the ovary and testis was approximately 2.0% for both sexes. The intersexuality was 42.2%, with the intersexuality among females being 49.5%, which was significantly higher than that of males (26.2%). Such findings indicate a negative impact on the reproductive output of the rock bream along the southern coast of Korea; however, a more detailed study is required to ascertain accurate causes.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"123-131"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a7/6f/dr-25-3-123.PMC8670776.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39621055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reproductive Cycle of Blue-Striped Angelfish, Chaetodontoplus septentrionalis in Jeju Coastal Waters. 济州岛近海蓝条纹天使鱼的繁殖周期。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.145

Dae-Jong Kim, Chi-Hoon Lee, Young-Don Lee

{"title":"Reproductive Cycle of Blue-Striped Angelfish, Chaetodontoplus septentrionalis in Jeju Coastal Waters.","authors":"Dae-Jong Kim, Chi-Hoon Lee, Young-Don Lee","doi":"10.12717/DR.2021.25.3.145","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.145","url":null,"abstract":"Reproductive cycle of the blue-striped angelfish, Chaetodontoplus septentrionalis were histologically investigated. Fish were monthly collected in the coastal waters of Munseom, Seogwipo, Jeju-Island from February to December 2018. The gonadosomatic index (GSI) increased from May and maintained high values in August. The reproductive cycle of female fish can be classified by the characteristics observed during gonadal development as followed: growing stage (November to June), early mature stage (May to June), mature and spawning stage (June to September), and degenerative and recovery stage (September to December). In the male, testicular development period was similar to that of ovarian development period, but mature and spawning period was one month longer from June to October. Fecundity of mature female ranged from 4,601 to 22,840 and was correlated positively with total length and body weight. The histological analysis of gonadal development indicated that the C. septentrionalis was summer-spawning type and is considered a multiple spawner during spawning season.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"145-155"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/6b/dr-25-3-145.PMC8670773.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39621057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0