Development & reproduction最新文献_第10页

Blood-Testis Barrier and Sperm Delayed in the Cauda Epididymis of the Reproductively Regressed Syrian Hamsters. 生殖退化叙利亚仓鼠附睾尾血睾丸屏障和精子延迟。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.1

Geon Hyung Jeon, Sung-Ho Lee, Yong-Pil Cheon, Donchan Choi

{"title":"Blood-Testis Barrier and Sperm Delayed in the Cauda Epididymis of the Reproductively Regressed Syrian Hamsters.","authors":"Geon Hyung Jeon, Sung-Ho Lee, Yong-Pil Cheon, Donchan Choi","doi":"10.12717/DR.2021.25.1.1","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.1","url":null,"abstract":"The Syrian (golden) hamsters are seasonal breeders whose reproductive functions are active in summer and inactive in winter. In experimental facility mimicking winter climate, short photoperiod (SP) induces gonadal regression. The blood-testis barrier (BTB) of the sexually involuted animals have been reported to be permeable, allowing developing germ cells to be engulfed or sloughed off the epithelium of the seminiferous tubules. The expressions of genes related to the tight junction composing of BTB were investigated in the reproductive active and inactive testes. Claudin-11, occludin, and junctional adhesion molecule (JAM) were definitely expressed in the active testes but not discernably detected in the inactive testes. And spermatozoa (sperm) were observed in the whole lengths of epididymides in the active testes. They were witnessed in only cauda region of the epididymides but not in caput and corpus regions in animals with the inactive testes. The results imply that the disorganization of BTB is associated with the testicular regression. The developing germ cells are swallowed into the Sertoli cells or travel into the lumen, as supported by the presence of the sperm delayed in the last region of the epididymis. These outcomes suggest that both apoptosis and desquamation are the processes that eliminate the germ cells during the regressing stage in the Syrian hamsters.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/76/63/dr-25-1-1.PMC8087257.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38971269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development of Porcine Somatic Cell Nuclear Transfer Embryos Following Treatment Time of Endoplasmic Reticulum Stress Inhibitor. 内质网应激抑制剂处理时间对猪体细胞核移植胚胎发育的影响。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.43

Mi-Jeong Kim, Bae-Dong Jung, Choon-Keun Park, Hee-Tae Cheong

{"title":"Development of Porcine Somatic Cell Nuclear Transfer Embryos Following Treatment Time of Endoplasmic Reticulum Stress Inhibitor.","authors":"Mi-Jeong Kim, Bae-Dong Jung, Choon-Keun Park, Hee-Tae Cheong","doi":"10.12717/DR.2021.25.1.43","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.43","url":null,"abstract":"We examine the effect of endoplasmic reticulum (ER) stress inhibitor treatment time on the in vitro development of porcine somatic cell nuclear transfer (SCNT) embryos. Porcine SCNT embryos were classified by four groups following treatment time of ER stress inhibitor, tauroursodeoxycholic acid (TUDCA; 100 μM); 1) non-treatment group (control), 2) treatment during micromanipulation process and for 3 h after fusion (NT+3 h group), 3) treatment only during in vitro culture after fusion (IVC group), and 4) treatment during micromanipulation process and in vitro culture (NT+IVC group). SCNT embryos were cultured for six days to examine the X-box binding protein 1 (Xbp1) splicing levels, the expression levels of ER stress-associated genes, oxidative stress-related genes, and apoptosis-related genes in blastocysts, and in vitro development. There was no significant difference in Xbp1 splicing level among all groups. Reduced expression of some ER stress-associated genes was observed in the treatment groups. The oxidative stress and apoptosis-related genes were significantly lower in all treatment groups than control (p<0.05). Although blastocyst development rates were not different among all groups (17.5% to 21.7%), the average cell number in blastocysts increased significantly in NT+3 h (48.5±2.3) and NT+IVC (47.7±2.4) groups compared to those of control and IVC groups (p<0.05). The result of this study suggests that the treatment of ER stress inhibitor on SCNT embryos from the micromanipulation process can improve the reprogramming efficiency of SCNT embryos by inhibiting the ER and oxidative stresses that may occur early in the SCNT process.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"43-53"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2b/25/dr-25-1-43.PMC8087258.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38972280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic Distances between Tailfin Anchovy (Coilia nasus) Populations Analyzed by PCR. 尾尾鱼(Coilia nasus)群体间遗传距离的PCR分析。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.59

Soo-Gun Jo, Jong-Man Yoon

{"title":"Genetic Distances between Tailfin Anchovy (Coilia nasus) Populations Analyzed by PCR.","authors":"Soo-Gun Jo, Jong-Man Yoon","doi":"10.12717/DR.2021.25.1.59","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.59","url":null,"abstract":"The author established a PCR-based genetic platform to examine the hierarchical polar dendrogram of Euclidean genetic distances of one tailfin anchovy population, especially for Coilia nasus, which was further associated with other fish population, by connecting with specifically designed oligonucleotide primer sets. Five oligonucleotide primers were used to generate a total of 260 and 211 scorable fragments in Coilia populations I and II, respectively. The DNA fragments ranged from greater than (approximately) 100 to more than 2,000 bp. The average bandsharing values (BS) of individuals from the anchovy population I (0.693) displayed higher values than individuals from population II (0.675). The genetic distance between individuals established the existence of a close relationship in group II. Comparatively, individuals of one anchovy population were fairly related to other fish populations, as shown in the polar hierarchical dendrogram of Euclidean genetic distances. The noteworthy genetic distance determined between two Coilia nasus populations demonstrates that this PCR technique can be applied as one of the several devices for individuals and/or population biological DNA researches undertaken for safeguarding species and for production of anchovies in the littoral area of Korea.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"59-65"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f1/86/dr-25-1-59.PMC8087260.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38972282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Nuclear DNA Content Determination of 31 Endemic Freshwater Fishes in Korea. 31种韩国特有淡水鱼的核DNA含量测定。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.25

In-Seok Park

引用次数: 0

Osteological Development of the Larvae and Juvenile of Favonigobius gymnauchen (Pisces:Gobiidae). 金鱼虾幼鱼及幼鱼的骨发育。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.33

Dong-Soo Jin, Jae-Min Park, Jeong-Ik Baek, Kyeong-Ho Han

{"title":"Osteological Development of the Larvae and Juvenile of Favonigobius gymnauchen (Pisces:Gobiidae).","authors":"Dong-Soo Jin, Jae-Min Park, Jeong-Ik Baek, Kyeong-Ho Han","doi":"10.12717/DR.2021.25.1.33","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.33","url":null,"abstract":"This study was carried out to observe the development of the autonomous skeletal development of the Favonigobius gymnauchen. Total length (TL) of larvae 3 days after hatching (DAH) were mean TL of 3.34 mm, with a line-shaped parasphenoid ossification in the cranium and basioccipital ossification in the back. The 10 DAH larvae had a mean TL of 5.20 mm, with the number of caudal vertebrae increasing to 15. The urostyle and two hypural bones in the lower part also began to ossify. The 23 DAH juveniles had a mean TL of 8.47 mm. The pectoral girdle's skeleton was completed as the scapula and coracoid were ossified. The pelvic girdle also fully supported the ventral fin as its ossification was completed. Favonigobius gymnauchen and Tridentiger obscurus showed similar characteristics in terms of the anus location of hatched larvae, number of myotomes, and melanophore distribution during the morphological development of the larvae and juveniles. However, this study confirmed differences in the development of the vertebrae and urostyle bone.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/cd/dr-25-1-33.PMC8087259.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38972279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas. 胰岛素样生长因子-1受体(IGF-1R)的表达和内源性底物的磷酸化导致太平洋牡蛎的成熟。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.67

Su-Jin Park, Youn Hee Choi

{"title":"Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas.","authors":"Su-Jin Park, Youn Hee Choi","doi":"10.12717/DR.2021.25.1.67","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.67","url":null,"abstract":"This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/b3/dr-25-1-67.PMC8087256.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38972283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization. 电压依赖性N型钙通道在小鼠卵子受精中的作用。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.297

Jin Hee Eum, Miseon Park, Jung Ah Yoon, Sook Young Yoon

{"title":"Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization.","authors":"Jin Hee Eum, Miseon Park, Jung Ah Yoon, Sook Young Yoon","doi":"10.12717/DR.2020.24.4.297","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.297","url":null,"abstract":"Repetitive changes in the intracellular calcium concentration ([Ca2+]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca2+]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca2+ ion elevation during [Ca2+]i oscillations are Ca2+ release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca2+ ion influx through Ca2+ channel on the plasma membrane. Ca2+ channels have been characterized into voltage-dependent Ca2+ channels (VDCCs), ligand-gated Ca2+ channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca2+]i oscillation was observed in a Ca2+ contained medium with sperm factor or adenophostin A injection but disappeared in Ca2+ free medium. Ca2+ influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca2+]i oscillation profiles in SrCl2 treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca2+ influx is essential for [Ca2+]i oscillation during mammalian fertilization. This Ca2+ influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"297-306"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic Differences in Natural and Cultured River Pufferfish Populations by PCR Analysis. 天然河豚与人工河豚群体遗传差异的PCR分析。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.327

Jong-Man Yoon

{"title":"Genetic Differences in Natural and Cultured River Pufferfish Populations by PCR Analysis.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2020.24.4.327","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.327","url":null,"abstract":"Genomic DNA (gDNA) extracted from two populations of natural and cultured river pufferfish (Takifugu obscurus) was amplified by polymerase chain reaction (PCR). The complexity of the fragments derived from the two locations varied dramatically. The genetic distances (GDs) between individuals numbered 15 and 12 in the cultured population was 0.053, which was the lowest acknowledged. The oligonucleotide primer OPC-11 identified 88 unique loci shared within each population reflecting the natural population. The OPC-05 primer identified 44 loci shared by the two populations. The average band-sharing (BS) values of individuals in the natural population (0.683±0.014) were lower than in those derived from the cultured population (0.759±0.009) (p<0.05). The shortest GD demonstrating a significant molecular difference was found between the cultured individuals # 15 and # 12 (GD=0.053). Individual # 02 of the natural population was most distantly related to cultured individual # 22 (GD=0.827). A cluster tree was built using the unweighted pair group method with arithmetic mean (UPGMA) Euclidean GD analysis based on a total of 578 various fragments derived from five primers in the two populations. Obvious markers identified in this study represent the genetic structure, species security, and proliferation of river pufferfish in the rivers of the Korean peninsula.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"327-336"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25328772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Early Life History of Lefua costata (Cypriniformes : Balitoridae) from Korea. 韩国长尾螺的早期生活史(鲤形目:鲤科)。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.307

Jae-Min Park, Seong-Jang Cho, Kyeong-Ho Han

引用次数: 0

YY1 and CP2c in Unidirectional Spermatogenesis and Stemness. YY1和CP2c在单向精子发生和干性中的作用。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.249

Yong-Pil Cheon, Donchan Choi, Sung-Ho Lee, Chul Geun Kim

{"title":"YY1 and CP2c in Unidirectional Spermatogenesis and Stemness.","authors":"Yong-Pil Cheon, Donchan Choi, Sung-Ho Lee, Chul Geun Kim","doi":"10.12717/DR.2020.24.4.249","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.249","url":null,"abstract":"Spermatogonial stem cells (SSCs) have stemness characteristics, including germ cell-specific imprints that allow them to form gametes. Spermatogenesis involves changes in gene expression such as a transition from expression of somatic to germ cell-specific genes, global repression of gene expression, meiotic sex chromosome inactivation, highly condensed packing of the nucleus with protamines, and morphogenesis. These step-by-step processes finally generate spermatozoa that are fertilization competent. Dynamic epigenetic modifications also confer totipotency to germ cells after fertilization. Primordial germ cells (PGCs) in embryos do not enter meiosis, remain in the proliferative stage, and are referred to as gonocytes, before entering quiescence. Gonocytes develop into SSCs at about 6 days after birth in rodents. Although chromatin structural modification by Polycomb is essential for gene silencing in mammals, and epigenetic changes are critical in spermatogenesis, a comprehensive understanding of transcriptional regulation is lacking. Recently, we evaluated the expression profiles of Yin Yang 1 (YY1) and CP2c in the gonads of E14.5 and 12-week-old mice. YY1 localizes at the nucleus and/or cytoplasm at specific stages of spermatogenesis, possibly by interaction with CP2c and YY1-interacting transcription factor. In the present article, we discuss the possible roles of YY1 and CP2c in spermatogenesis and stemness based on our results and a review of the relevant literature.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"249-262"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2