Development & reproduction最新文献

Regulation of Transcriptional Coactivator with PDZ-Binding Motif (TAZ) Expression by Estrogen in the Mouse Uterine Endometrium. 雌激素对小鼠子宫内膜pdz结合基序转录共激活因子（TAZ）表达的调控。

Development & reproduction Pub Date : 2025-06-01 Epub Date: 2025-06-30 DOI: 10.12717/DR.2025.29.2.31

Semi Hwang, Byeongseok Kim, Johee Kim, Yeonju Suh, Jimin Lee, Sangok Park, Ok-Hee Lee, ManRyeol Lee, Youngsok Choi

{"title":"Regulation of Transcriptional Coactivator with PDZ-Binding Motif (TAZ) Expression by Estrogen in the Mouse Uterine Endometrium.","authors":"Semi Hwang, Byeongseok Kim, Johee Kim, Yeonju Suh, Jimin Lee, Sangok Park, Ok-Hee Lee, ManRyeol Lee, Youngsok Choi","doi":"10.12717/DR.2025.29.2.31","DOIUrl":"10.12717/DR.2025.29.2.31","url":null,"abstract":"Transcriptional coactivator with PDZ-binding motif (TAZ) functions as a transcriptional coactivator, which shuttles between the cytoplasm and the nucleus under the Hippo signaling. It is known to be involved in promoting cell proliferation, organ overgrowth, survival to stress, and dedifferentiation by interacting with TEAD transcription factors (TEADs). However, the regulation of TAZ by intrauterine hormones has not yet been investigated. In this study, we investigated TAZ expression during the estrous cycle in the normal mouse uterus and the effect of estrogen and progesterone on TAZ expression in the ovariectomized (OVX) mouse uterus. TAZ expression levels did not show a statistically significant change in the uterus during the estrous cycle. However, immunofluorescence revealed that TAZ nuclear localization significantly increased at the estrus stage. In the OVX mouse uterus, the expression levels of TAZ mRNA and protein dramatically increased in a time-dependent manner after estrogen treatment. Also, immunofluorescence showed that the nuclear TAZ expression increased at 6 h and 12 h after estrogen treatment compared to the oil treated OVX mouse uterus (0 h). Finally, pretreatment of an estrogen receptor (ER) antagonist ICI 182,780 efficiently reduced estrogen-induced TAZ expression. However, progesterone did not significantly affect the expression of TAZ in both mRNA and protein levels. In conclusion, TAZ expression is regulated and activated by estrogen through nuclear estrogen receptors, ERα, and ERβ in the uterine environment.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"29 2","pages":"31-46"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomy and Function of Deleted in Azoospermia Like (DAZL) Gene in Human and Mouse. 人和小鼠无精子症样缺失（DAZL）基因的解剖和功能。

Development & reproduction Pub Date : 2025-06-01 Epub Date: 2025-06-30 DOI: 10.12717/DR.2025.29.2.19

HeeJi Choi, Yong-Pil Cheon

{"title":"Anatomy and Function of Deleted in Azoospermia Like (DAZL) Gene in Human and Mouse.","authors":"HeeJi Choi, Yong-Pil Cheon","doi":"10.12717/DR.2025.29.2.19","DOIUrl":"10.12717/DR.2025.29.2.19","url":null,"abstract":"The deleted in azoospermia like (DAZL) gene is a member of the DAZ gene family. It is firstly identified in male germ cells and recognized as a key molecule of their development, now it is extended to the female germ cells and the embryo. The DAZL gene is constructed with 11 exons, 10 introns, a 5' untranslated region (UTR), and a 3' UTR, and the enhancers at the upstream of the promoter in both human and mouse. It has been revealed that DAZL gene expression is not restricted to germ cells. The known mechanisms for expression regulation include the CpG methylation on the promoter region and post-transcriptional regulation by antagonistic proteins. DAZL protein has one RNA recognition motif (RRM) and one DAZ repeat. DAZL orchestrates the translation of numerous mRNAs essential for germ cell proliferation, differentiation, and survival. Several studies have unveiled DAZL's broader roles, including its involvement in stemness and tumorigenicity through post-transcriptional regulation via polyadenylation and potential functions in RNA stabilization. The alternatively spliced variants are also evaluated in different tissues. This review consolidates current knowledge on DAZL's molecular mechanisms, expression, and emerging research directions, and introduces DAZL gene anatomy.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"29 2","pages":"19-30"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-932 Suppresses the Expression of Germline-Specific vasa in Somatic Drosophila Testis Hub Cells. miR-932抑制生殖系特异性输精管在体细胞果蝇睾丸中枢细胞中的表达

Development & reproduction Pub Date : 2025-06-01 Epub Date: 2025-06-30 DOI: 10.12717/DR.2025.29.2.55

Jin A Lee, Wijeong Jang, Young Chul Lee, Changsoo Kim

引用次数: 0

Effects of Nonylphenol on the Secretions of Catecholamines, Gonadotropin-Releasing Hormone (GnRH) and Kisspeptin from Short-Term Incubated Rat Hypothalamus. 壬基酚对短期培养大鼠下丘脑儿茶酚胺、促性腺激素释放激素（GnRH）和Kisspeptin分泌的影响。

Development & reproduction Pub Date : 2025-06-01 Epub Date: 2025-06-30 DOI: 10.12717/DR.2025.29.2.47

Hee-Soo Kim, Sung-Ho Lee

{"title":"Effects of Nonylphenol on the Secretions of Catecholamines, Gonadotropin-Releasing Hormone (GnRH) and Kisspeptin from Short-Term Incubated Rat Hypothalamus.","authors":"Hee-Soo Kim, Sung-Ho Lee","doi":"10.12717/DR.2025.29.2.47","DOIUrl":"10.12717/DR.2025.29.2.47","url":null,"abstract":"Previously, we developed a short-term incubation method of rat adrenal and demonstrated that nonylphenol (NP) exposure could induce changes in secretions of adrenal hormones. In the present study, the effects of NP on changes in hormonal secretion from hypothalamus were investigated. The catecholamine levels were measured using high-performance liquid chromatography with electrochemical detection (HPLC-ECD) and the levels of gonadotropin-releasing hormone (GnRH) and kisspeptin were measured using enzyme-linked immunosorbent assay (ELISA). The norepinephrine (NE) levels from NP-treated male and female hypothalamus were not significantly changed across the entire treatment concentration (from 1 nM to 1 μM), except male 10 nM-treated group which were significantly lower than control (p<0.05). The epinephrine (E) levels from NP-treated female hypothalamus were significantly increased in 100 pM- and 1 nM-trated group (p<0.05). However, the E levels from NP-treated male hypothalamus were significantly decreased in 100 pM- and 10 nM-treated group (p<0.05). The GnRH levels from NP-treated hypothalamus showed an increasing trend, especially significant in male 10 nM- and 100 nM-treated groups (p<0.001) and female 1 nM-, 100 nM- and 1 μM-trated groups (p<0.05, p<0.01 and p<0.05, respectively). Also, the kisspeptin levels in incubated media of both sexes showed a strong increasing trend, especially significant in male 1 nM- and 10 nM-treated groups (p<0.05) and all of female groups except 10 nM-treated. In conclusion, our incubation method could be quite suitable for rapidly and effectively measuring endocrine disrupting chemicals (EDC) activity in hypothalamus. NP treatment shown stimulatory effects on both GnRH and kisspeptin secretions from hypothalamus of both sexes, suggesting possible relationship between NP exposure and reproductive phenomena and related disorders.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"29 2","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12285544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repeated Gonadotropin Administration Suppresses T Cell Development in the Mouse Thymus. 反复给药促性腺激素抑制小鼠胸腺T细胞发育。

Development & reproduction Pub Date : 2025-03-01 Epub Date: 2025-03-31 DOI: 10.12717/DR.2025.29.1.1

Jin Yoon, Sojung Sun, Soeun Moon, Hyunwon Yang

{"title":"Repeated Gonadotropin Administration Suppresses T Cell Development in the Mouse Thymus.","authors":"Jin Yoon, Sojung Sun, Soeun Moon, Hyunwon Yang","doi":"10.12717/DR.2025.29.1.1","DOIUrl":"https://doi.org/10.12717/DR.2025.29.1.1","url":null,"abstract":"Gonadotropins, such as follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG), are widely used to induce ovarian hyperovulation during in vitro fertilization and embryo transfer (IVF-ET) for the treatment of infertility. However, the effects of repeated administration of these gonadotropins on immune function, particularly on T cell development in the thymus, remain poorly understood. This study investigated the effects of repeated administration of pregnant mare serum gonadotropin (PMSG) and hCG on thymic T cell development in mice. Histological analysis revealed structural changes in the thymus, including a blurred boundary between the medulla and cortex and reduced vascularization after repeated administration of PMSG and hCG. Quantitative real-time PCR showed increased expression of adipogenesis-related genes [phosphoenolpyruvate carboxykinase (PEPCK), adipocyte fatty acid-binding protein 2 (aP2), peroxisome proliferator-activated receptor gamma (PPARγ)] but no significant changes in thymic epithelial cell-related genes [autoimmune regulator (AIRE), epithelial V-like antigen (EVA), interleukin 7 (IL-7)]. Flow cytometry revealed a decrease in CD4+CD8+ T cells and an increase in CD4-CD8-T cells with altered CD25/CD44 subsets. In addition, CD4+ and CD8+ T cells in the spleen were significantly reduced. These findings suggest that repeated gonadotropin exposure may disrupt thymic T cell development and peripheral T cell populations, potentially impairing immune function. Further research is needed to elucidate the underlying mechanisms and broader immunologic consequences of gonadotropin use in infertility treatment.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"29 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric Assessment of Egg Quality for Second Generation of Cultured Small Yellow Croaker, Larimichthys polyactis Broodstock. 小黄鱼二代养殖鱼卵品质的形态计量学评价。

Development & reproduction Pub Date : 2025-03-01 Epub Date: 2025-03-31 DOI: 10.12717/DR.2025.29.1.13

In Joon Hwang, Hee Woong Kang

{"title":"Morphometric Assessment of Egg Quality for Second Generation of Cultured Small Yellow Croaker, Larimichthys polyactis Broodstock.","authors":"In Joon Hwang, Hee Woong Kang","doi":"10.12717/DR.2025.29.1.13","DOIUrl":"https://doi.org/10.12717/DR.2025.29.1.13","url":null,"abstract":"The egg quality is a representative limiting factor in developing culture techniques for certain fish species. It is a known predictor of subsequent larval viability, quality, and stress resistance related to aquaculture productivity. Here we tracked egg quality in a second generation of broodstock small yellow croacker, Larimichthys polyactis. Cultured second generation broodstock (3 years old, 600 fishes) was reared in indoor tank (30 tons). We induced natural spawning with increasing water temperature (11.5°C22.0°C) and regulation of photoperiod (9L:15D). We used spawning events from spawning period and monitored basic morphometrics such as: egg viability, egg diameter (ED), oil droplet diameter (OD) and oil droplet volume. Natural spawning of the broodstock was maintained for 23 days. EDs and OD for L. polyactis decreased as the spawning season progressed and water temperature increased. We showed that smaller eggs lead to higher quality with viability, and that using eggs later in the spawning season would lead to better production. In addition, the volume of oil droplet was the strongest factors for prediction of egg viability for cultured second generation of small yellow croaker.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"29 1","pages":"13-18"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Different Regional Characteristics of Spawning and Growing Sites on Growth and Taste of Pacific Oyster, Crassostrea gigas. 产卵和生长地点不同地域特征对太平洋牡蛎、长牡蛎生长和口感的影响。

Development & reproduction Pub Date : 2024-12-01 Epub Date: 2024-12-31 DOI: 10.12717/DR.2024.28.4.175

Ji-Sung Moon, Hee-Jung Lee, Si-Chan Kim, Eun-Seo Lee, Josel Cadangin, Bo Hyun Joo, Su-Jin Park, Young Baek Hur, Taek-Jeong Nam, Youn Hee Choi

{"title":"Effect of Different Regional Characteristics of Spawning and Growing Sites on Growth and Taste of Pacific Oyster, Crassostrea gigas.","authors":"Ji-Sung Moon, Hee-Jung Lee, Si-Chan Kim, Eun-Seo Lee, Josel Cadangin, Bo Hyun Joo, Su-Jin Park, Young Baek Hur, Taek-Jeong Nam, Youn Hee Choi","doi":"10.12717/DR.2024.28.4.175","DOIUrl":"10.12717/DR.2024.28.4.175","url":null,"abstract":"While Pacific oysters are important commercial aquaculture species worldwide, the effect of hormonal regulation and environmental conditions on growth and taste profile have not been fully known. Insulin-like growth factor (IGF) systems are known to play a major role in regulating neuroendocrine functions across various physiological processes and are particularly involved in growth. IGFs expression also is directly related to the nutritional status of vertebrates, however, full mechanism has not been clearly identified in bivalves. In this study, differences in growth, IGFs expression, and taste according to cultivation site of Pacific oysters were investigated. Oysters were collected in three different spawning sites located on the south coast of Korea in July 2022 and hardened until June 2023. Then, the oysters were cultured in two different growing sites (Tongyeong site, TS; Geoje site, GS) for six months. The total weight of oysters, along with their condition index and tissue weight rate, was significantly higher in TS. Additionally, IGF expression was higher in TS during most of the sampled months. However, oysters from the GS scored higher in taste evaluations. The IGFs system in oysters shows a similar trend to previous studies, with higher levels in faster-growing individuals, suggesting oysters in TS were more adequately nourished by the surrounding environment in this research. However, in taste evaluation, oysters from the GS showed better results than those from the TS. Despite these results, determining whether one site is superior in certain aspects is still not fully possible, which warrants further investigations.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"28 4","pages":"175-186"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatty Acid Synthase (FASN) Inhibitors Suppress Metformin-Induced Fat Accumulation and Apoptosis in H4IIE Hepatocellular Carcinoma Cells. 脂肪酸合成酶（FASN）抑制剂抑制二甲双胍诱导的H4IIE肝癌细胞脂肪堆积和凋亡

Development & reproduction Pub Date : 2024-12-01 Epub Date: 2024-12-31 DOI: 10.12717/DR.2024.28.4.163

Deokbae Park, Hyejin Boo

{"title":"Fatty Acid Synthase (FASN) Inhibitors Suppress Metformin-Induced Fat Accumulation and Apoptosis in H4IIE Hepatocellular Carcinoma Cells.","authors":"Deokbae Park, Hyejin Boo","doi":"10.12717/DR.2024.28.4.163","DOIUrl":"10.12717/DR.2024.28.4.163","url":null,"abstract":"We previously reported that metformin, a widely prescribed antidiabetic drug, induces the accumulation of triglyceride (TG) together with the apoptotic death of H4IIE via AMP-activated protein kinase (AMPK) in hepatocellular carcinoma (HCC) cells. However, the effect of cytoplasmic fat accumulation on the growth of HCCs remains controversial. Herein, we investigated the effect of fatty acid synthase (FASN) inhibitors on the basal- or metformin-induced changes including the content of cytoplasmic TG and the viability of HCC cells. Cerulenin and C75, inhibitors of FASN, did not significantly affect the basal TG content but dose-dependently suppressed the metformin-induced increase in the cytoplasmic TG content. Metformin-induced apoptosis of H4IIE cells was also significantly reduced by cerulenin and C75. Metformin enhanced the generation of reactive oxygen species which was suppressed by adding cerulenin or T75. Cerulenin also stimulated cell migration, which was suppressed by metformin. However, the degree of suppressive effect of metformin on TG synthesis, apoptosis, and cell migration was much more prominent by the inhibition of AMPK by compound C than cerulenin. In conclusion, our study found that excess fat accumulation is responsible for the apoptosis of H4IIE HCC cells and is informative for designing anti-tumor reagents, especially in HCC.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"28 4","pages":"163-174"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signal Transduction of Constitutive Activating and Inactivating Eel Lutropin/Choriogonadotropin Receptor (Eel LH/CGR) Mutants by Recombinant Equine Chorionic Gonadotropin (Rec-eCG). 重组马绒毛膜促性腺激素（Rec-eCG）对组成型黄鳝促性腺激素/绒毛膜促性腺激素受体（LH/CGR）突变体激活和失活的信号传导

Development & reproduction Pub Date : 2024-12-01 Epub Date: 2024-12-31 DOI: 10.12717/DR.2024.28.4.141

Kwan-Sik Min, Sei Hyen Park, Ha-Rin Jeong, Jae-Hyek Park, Munkhzaya Byambaragchaa

{"title":"Signal Transduction of Constitutive Activating and Inactivating Eel Lutropin/Choriogonadotropin Receptor (Eel LH/CGR) Mutants by Recombinant Equine Chorionic Gonadotropin (Rec-eCG).","authors":"Kwan-Sik Min, Sei Hyen Park, Ha-Rin Jeong, Jae-Hyek Park, Munkhzaya Byambaragchaa","doi":"10.12717/DR.2024.28.4.141","DOIUrl":"10.12717/DR.2024.28.4.141","url":null,"abstract":"Lutropin/choriogonadotropin receptor (LH/CGR) is a member of the G protein-coupled receptor superfamily. LH/CGRs in fish and mammalian species have been reported to contain naturally occurring, constitutively activating, and inactivating mutations in highly conserved regions. The present study was designed to determine the functional aspect of eel LH/CGR signal transduction. Biochemical analysis was performed using cells transfected with wild-type eel LH/CG (eel LH/CGR-wt) or with activating (designated eel LH/CGR-M410T, L469R, and D590Y) and inactivating (eel LH/CGR-D 417N and Y558F) mutants. We also generated a mutant (eel LH/CGR-t651) in which the C-terminal cytoplasmic tail was truncated at residue 651. Activating mutant cells expressing eel LH/CGR-M410T, L469R, and D590Y exhibited 1.4-, 8.7-, and 4.0-fold increases in the basal cAMP response, respectively, without recombinant equine chorionic gonadotropin (rec-eCG) agonist treatment. In inactivating mutants (eel LH/CGR-D417N and Y558F), the cyclic adenosine monophosphate (cAMP) response did not result in completely impaired signal transduction. However, the eel LH/CGR-t651 mutant did not exhibit any cAMP signaling following high-agonist treatment. Rmax values did not increase with further rec-eCG agonist stimulation. Our results suggest that constitutively activating and inactivating eel LH/CGR mutants with highly conserved amino acids exhibit a significant signal transduction pathway for glycoprotein hormone receptors. Eel LH/CGRs in activating and inactivating mutants are usually processed by receptor-mediated signaling following rec-eCG agonist stimulation.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"28 4","pages":"141-151"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zebrafish PEX1 Is Required for the Generation of GABAergic Neuron in p3 Domain. 斑马鱼p3区域gaba能神经元的生成需要PEX1。

Development & reproduction Pub Date : 2024-12-01 Epub Date: 2024-12-31 DOI: 10.12717/DR.2024.28.4.129

Jae-Ho Ryu, Minjung Kim, Aseung Kim, Hyunju Ro, Seok-Hyung Kim, Sang-Yeob Yeo

{"title":"Zebrafish PEX1 Is Required for the Generation of GABAergic Neuron in p3 Domain.","authors":"Jae-Ho Ryu, Minjung Kim, Aseung Kim, Hyunju Ro, Seok-Hyung Kim, Sang-Yeob Yeo","doi":"10.12717/DR.2024.28.4.129","DOIUrl":"10.12717/DR.2024.28.4.129","url":null,"abstract":"Maintenance of neural progenitors requires Notch signaling in vertebrate development. Previous study has shown that Jagged2-mediated Notch signaling maintains proliferating neural progenitors in the ventral spinal cord. However, components for Jagged-mediated signaling remain poorly defined during late neurogenesis. Here we performed yeast-two hybrid screening by using the intracellular domain (ICD) of zebrafish Jagged2, and investigated a possible role of PEX1 as a component of Notch signaling for the cell-fate decision and the differentiation of neural precursors in p3 domain. Western blotting showed that zebrafish PEX1 might interacts with the ICD of zebrafish Jagged2 physically. PEX1 morpholino-injected embryos showed the increased number of GABAergic KA\" neurons as well as the ectopic expression of secondary motor neurons in the p3 domain. The increased number of KA\" neurons was also observed in the zebrafish embryos with PEX1 mutation induced by CRISPR/Cas9. These phenotypes resemble with that of Jagged2 morphant. Our observations imply that a critical role of PEX1 in the cell-fate decision of proliferating neural precursors in the p3 domain during the continuing growth and development of the vertebrate nervous system.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"28 4","pages":"129-139"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0