Development & reproduction最新文献_第9页

The Nuclear DNA Content Determination of 31 Endemic Freshwater Fishes in Korea. 31种韩国特有淡水鱼的核DNA含量测定。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.25

In-Seok Park

引用次数: 0

Osteological Development of the Larvae and Juvenile of Favonigobius gymnauchen (Pisces:Gobiidae). 金鱼虾幼鱼及幼鱼的骨发育。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.33

Dong-Soo Jin, Jae-Min Park, Jeong-Ik Baek, Kyeong-Ho Han

{"title":"Osteological Development of the Larvae and Juvenile of Favonigobius gymnauchen (Pisces:Gobiidae).","authors":"Dong-Soo Jin, Jae-Min Park, Jeong-Ik Baek, Kyeong-Ho Han","doi":"10.12717/DR.2021.25.1.33","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.33","url":null,"abstract":"This study was carried out to observe the development of the autonomous skeletal development of the Favonigobius gymnauchen. Total length (TL) of larvae 3 days after hatching (DAH) were mean TL of 3.34 mm, with a line-shaped parasphenoid ossification in the cranium and basioccipital ossification in the back. The 10 DAH larvae had a mean TL of 5.20 mm, with the number of caudal vertebrae increasing to 15. The urostyle and two hypural bones in the lower part also began to ossify. The 23 DAH juveniles had a mean TL of 8.47 mm. The pectoral girdle's skeleton was completed as the scapula and coracoid were ossified. The pelvic girdle also fully supported the ventral fin as its ossification was completed. Favonigobius gymnauchen and Tridentiger obscurus showed similar characteristics in terms of the anus location of hatched larvae, number of myotomes, and melanophore distribution during the morphological development of the larvae and juveniles. However, this study confirmed differences in the development of the vertebrae and urostyle bone.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/cd/dr-25-1-33.PMC8087259.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38972279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas. 胰岛素样生长因子-1受体(IGF-1R)的表达和内源性底物的磷酸化导致太平洋牡蛎的成熟。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.67

Su-Jin Park, Youn Hee Choi

{"title":"Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas.","authors":"Su-Jin Park, Youn Hee Choi","doi":"10.12717/DR.2021.25.1.67","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.67","url":null,"abstract":"This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/b3/dr-25-1-67.PMC8087256.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38972283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization. 电压依赖性N型钙通道在小鼠卵子受精中的作用。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.297

Jin Hee Eum, Miseon Park, Jung Ah Yoon, Sook Young Yoon

{"title":"Voltage Dependent N Type Calcium Channel in Mouse Egg Fertilization.","authors":"Jin Hee Eum, Miseon Park, Jung Ah Yoon, Sook Young Yoon","doi":"10.12717/DR.2020.24.4.297","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.297","url":null,"abstract":"Repetitive changes in the intracellular calcium concentration ([Ca2+]i) triggers egg activation, including cortical granule exocytosis, resumption of second meiosis, block to polyspermy, and initiating embryonic development. [Ca2+]i oscillations that continue for several hours, are required for the early events of egg activation and possibly connected to further development to the blastocyst stage. The sources of Ca2+ ion elevation during [Ca2+]i oscillations are Ca2+ release from endoplasmic reticulum through inositol 1,4,5 tri-phosphate receptor and Ca2+ ion influx through Ca2+ channel on the plasma membrane. Ca2+ channels have been characterized into voltage-dependent Ca2+ channels (VDCCs), ligand-gated Ca2+ channel, and leak-channel. VDCCs expressed on muscle cell or neuron is specified into L, T, N, P, Q, and R type VDCs by their activation threshold or their sensitivity to peptide toxins isolated from cone snails and spiders. The present study was aimed to investigate the localization pattern of N and P/Q type voltage-dependent calcium channels in mouse eggs and the role in fertilization. [Ca2+]i oscillation was observed in a Ca2+ contained medium with sperm factor or adenophostin A injection but disappeared in Ca2+ free medium. Ca2+ influx was decreased by Lat A. N-VDCC specific inhibitor, ω-Conotoxin CVIIA induced abnormal [Ca2+]i oscillation profiles in SrCl2 treatment. N or P/Q type VDC were distributed on the plasma membrane in cortical cluster form, not in the cytoplasm. Ca2+ influx is essential for [Ca2+]i oscillation during mammalian fertilization. This Ca2+ influx might be controlled through the N or P/Q type VDCCs. Abnormal VDCCs expression of eggs could be tested in fertilization failure or low fertilization eggs in subfertility women.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"297-306"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Early Life History of Lefua costata (Cypriniformes : Balitoridae) from Korea. 韩国长尾螺的早期生活史(鲤形目:鲤科)。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.307

Jae-Min Park, Seong-Jang Cho, Kyeong-Ho Han

引用次数: 0

Genetic Differences in Natural and Cultured River Pufferfish Populations by PCR Analysis. 天然河豚与人工河豚群体遗传差异的PCR分析。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.327

Jong-Man Yoon

{"title":"Genetic Differences in Natural and Cultured River Pufferfish Populations by PCR Analysis.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2020.24.4.327","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.327","url":null,"abstract":"Genomic DNA (gDNA) extracted from two populations of natural and cultured river pufferfish (Takifugu obscurus) was amplified by polymerase chain reaction (PCR). The complexity of the fragments derived from the two locations varied dramatically. The genetic distances (GDs) between individuals numbered 15 and 12 in the cultured population was 0.053, which was the lowest acknowledged. The oligonucleotide primer OPC-11 identified 88 unique loci shared within each population reflecting the natural population. The OPC-05 primer identified 44 loci shared by the two populations. The average band-sharing (BS) values of individuals in the natural population (0.683±0.014) were lower than in those derived from the cultured population (0.759±0.009) (p<0.05). The shortest GD demonstrating a significant molecular difference was found between the cultured individuals # 15 and # 12 (GD=0.053). Individual # 02 of the natural population was most distantly related to cultured individual # 22 (GD=0.827). A cluster tree was built using the unweighted pair group method with arithmetic mean (UPGMA) Euclidean GD analysis based on a total of 578 various fragments derived from five primers in the two populations. Obvious markers identified in this study represent the genetic structure, species security, and proliferation of river pufferfish in the rivers of the Korean peninsula.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"327-336"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25328772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

YY1 and CP2c in Unidirectional Spermatogenesis and Stemness. YY1和CP2c在单向精子发生和干性中的作用。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.249

Yong-Pil Cheon, Donchan Choi, Sung-Ho Lee, Chul Geun Kim

{"title":"YY1 and CP2c in Unidirectional Spermatogenesis and Stemness.","authors":"Yong-Pil Cheon, Donchan Choi, Sung-Ho Lee, Chul Geun Kim","doi":"10.12717/DR.2020.24.4.249","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.249","url":null,"abstract":"Spermatogonial stem cells (SSCs) have stemness characteristics, including germ cell-specific imprints that allow them to form gametes. Spermatogenesis involves changes in gene expression such as a transition from expression of somatic to germ cell-specific genes, global repression of gene expression, meiotic sex chromosome inactivation, highly condensed packing of the nucleus with protamines, and morphogenesis. These step-by-step processes finally generate spermatozoa that are fertilization competent. Dynamic epigenetic modifications also confer totipotency to germ cells after fertilization. Primordial germ cells (PGCs) in embryos do not enter meiosis, remain in the proliferative stage, and are referred to as gonocytes, before entering quiescence. Gonocytes develop into SSCs at about 6 days after birth in rodents. Although chromatin structural modification by Polycomb is essential for gene silencing in mammals, and epigenetic changes are critical in spermatogenesis, a comprehensive understanding of transcriptional regulation is lacking. Recently, we evaluated the expression profiles of Yin Yang 1 (YY1) and CP2c in the gonads of E14.5 and 12-week-old mice. YY1 localizes at the nucleus and/or cytoplasm at specific stages of spermatogenesis, possibly by interaction with CP2c and YY1-interacting transcription factor. In the present article, we discuss the possible roles of YY1 and CP2c in spermatogenesis and stemness based on our results and a review of the relevant literature.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"249-262"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Susceptibility of Koi, Koi×Red Common Carp, and Red Common Carp×Koi to Koi Herpesvirus (KHV). 锦鲤、锦鲤×红鲤和红鲤×锦鲤对锦鲤疱疹病毒（KHV）的易感性。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.277

Ju-Ae Hwang, Jung Eun Kim, Hyeong Su Kim, Junseong Park, Jeong-Ho Lee

{"title":"Susceptibility of Koi, Koi×Red Common Carp, and Red Common Carp×Koi to Koi Herpesvirus (KHV).","authors":"Ju-Ae Hwang, Jung Eun Kim, Hyeong Su Kim, Junseong Park, Jeong-Ho Lee","doi":"10.12717/DR.2020.24.4.277","DOIUrl":"10.12717/DR.2020.24.4.277","url":null,"abstract":"The disease-causing koi herpes virus (KHV), also known as cyprinid herpesvirus-3 (CyHV-3), causes mass mortality of koi and carp. Koi (Cyprinus carpio) is a host for KHV, one of 12 virus species in the Alloherpesviridae family. We examined the effects of KHV disease koi (KK), and on koi×red common carp (KR) and red common carp×koi (RK) cross, using a virus challenge test. The infected fish had clinical signs that included gill necrosis and skin lesions. The RK and KR were highly more resistant (cumulative mortality: RK; 6% and KR; 8%) to KHV infection than KK fish (cumulative mortality: 28%). KHV DNA was confirmed in the tissues of all dead fish in groups by use of polymerase chain reaction (PCR), and the presence of the KHV protein in kidney was confirmed by immunohistochemistry. Histological analysis showed severe gill lesions and fusion of the lamellae in KK fish, but less severe damage in RK fish. In immunohistochemistry analysis, the KHV protein localized in the cytoplasm of infected kidney cells of KK, but the cross groups had lower levels of KHV antigen. Our data indicate that the cross groups had increased resistance to KHV disease.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"277-286"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Adipocyte Differentiation and Maturation-related Gene Expression in the Epididymal Fat of Estrogen Receptor α Knockout (ERαKO) Mouse during Postnatal Development Period. 雌激素受体α敲除(ERαKO)小鼠产后发育过程中附睾脂肪脂肪细胞分化及成熟相关基因表达的评估。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.287

Yong-Pil Cheon, CheMyong Ko, Ki-Ho Lee

{"title":"Assessment of Adipocyte Differentiation and Maturation-related Gene Expression in the Epididymal Fat of Estrogen Receptor α Knockout (ERαKO) Mouse during Postnatal Development Period.","authors":"Yong-Pil Cheon, CheMyong Ko, Ki-Ho Lee","doi":"10.12717/DR.2020.24.4.287","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.287","url":null,"abstract":"The absence of functional estrogen receptor α (Esr1) results in an overgrowth of the epididymal fat, as observed in estrogen receptor α knockout (ERαKO) mouse. The present research was aimed to evaluate expression of various molecules associated with adipocyte differentiation and maturation in the epididymal fat of ERαKO mouse at several postnatal ages by using quantitative real-time polymerase chain reaction. The highest transcript levels of all molecules were detected at 12 months of postnatal age, except leptin which the mRNA level was increased at 5 months of age and was unchanged until 12 months of age. The expression levels of CCAAT enhancer binding protein (Cebp) alpha, androgen receptor, and lipoprotein lipase were decreased at 5 months of age but increased at about 8 months of age. The mRNA levels of Cebp gamma and sterol regulatory element binding transcription factor 1 remained steady until 8 months of age. Continuous increases of transcript levels during postnatal period were found in Cebp beta, estrogen receptor (ER) beta, fatty acid binding protein 4, and delta like non-canonical Notch ligand 1. The increases of peroxisome proliferator-activated receptor gamma and adiponectin mRNA levels were detected as early as 8 months of age. The levels of fatty acid synthase and resistin transcript at 5 and 8 months of age were lower than that at 2 months of age. These findings show the aberrant expression patterns of genes related to adipocyte differentiation and maturation in the postnatal epididymal fat pad by the disruption of ER alpha function.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"287-296"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic Variability Comparison of Wild and Cultured Far Eastern Catfish (Silurus asotus) of Korea using Microsatellite Marker. 利用微卫星标记比较韩国野生和养殖远东鲶鱼的遗传变异。

Development & reproduction Pub Date : 2020-12-01 Epub Date: 2020-12-31 DOI: 10.12717/DR.2020.24.4.317

Jung Eun Kim, Ju-Ae Hwang, Hyeong Su Kim, Jeong-Ho Lee

{"title":"Genetic Variability Comparison of Wild and Cultured Far Eastern Catfish (Silurus asotus) of Korea using Microsatellite Marker.","authors":"Jung Eun Kim, Ju-Ae Hwang, Hyeong Su Kim, Jeong-Ho Lee","doi":"10.12717/DR.2020.24.4.317","DOIUrl":"https://doi.org/10.12717/DR.2020.24.4.317","url":null,"abstract":"The Far Eastern catfish (Silurus asotus) is an important commercial freshwater fish in Korea. Investigation of the genetic diversity of wild and cultured domestic catfish groups is essential for the restoration of fishery resources and for increasing local revenue. However, there are relatively few genetic diversity studies on wild and cultured catfish in Korea. In the present study, we analyzed the genetic diversity and association of wild and cultured catfish using five microsatellite markers. We determined that the number of alleles per locus (NA ) ranged from 9 to 25, wherein the Jeonbuk catfish demonstrated the highest mean number of alleles per locus and the cultured catfish exhibited the lowest. The average expected heterozygosity (He) of the wild catfish samples was 0.907, and that of the cultured catfish showed was 0.875. The genetic distances (GD value) among populations of all catfish ranged from 0.138 to 0.242. Jeonnam and Jeonbuk wild catfish were located closest to each other, and the cultured group was separated from the other groups. In conclusion, the present study confirmed that the genetic diversity of wild and cultured catfish was maintained at a high level. In the case of the wild group, it is effective in maintaining diversity due to the continuous fry release by the local fish research institute. However, the genetic diversity of cultured catfish declined. Low diversity is associated with slow growth and weakened immunity, and therefore continuous monitoring is necessary.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"24 4","pages":"317-326"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/73/bb/dr-24-4-317.PMC7837421.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25328771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0