Development & reproduction最新文献_第9页

Genetic Variations within and between Blue Crab (Portunus trituberculatus) Groups. 蓝蟹（Portunus trituberculatus）群体内部和群体之间的遗传变异。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.185

Young-Jae Song, Jong-Man Yoon

{"title":"Genetic Variations within and between Blue Crab (Portunus trituberculatus) Groups.","authors":"Young-Jae Song, Jong-Man Yoon","doi":"10.12717/DR.2021.25.3.185","DOIUrl":"10.12717/DR.2021.25.3.185","url":null,"abstract":"Abstract The five oligonucleotide primers (oligo-primers) turned out a total of 335 fragments (FMs) (52.9%) in the blue crab (Portunus trituberculatus) group alpha and 298 FMs (47.1%) in the crab group beta, with the FM scales range varying from 100 bp to 2,000 bp. The highest band-sharing (BS) value (0.907) was found between individual’s no. 19 and no. 20 within the blue crab group beta. Parties in the blue crab group beta (0.601±0.017) had higher BS rates than did parties from the crab group alpha (0.563±0.017) (p<0.05). The polar dendrogram got by the five oligo-primers points out two genetic extents: bundle I (BLUECRAB 01, 03, 04, 05, 06, 08, and 10) and bundle II (BLUECRAB 02, 07, 09. 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The OPD-01 primer revealed 22 loci shared by all the examples of the as FMs of 1,000 bp. The oligo-primer OPA-05 made unique loci shared to each group (ULSEG), almost 400 bp and 500 bp, individually, in blue crab group beta. The remaining oligo-primers did not reveal any loci shared by the two crab groups (LSTG). The average number of ULSEG was diverse and 1.6-fold higher in the crab group beta than in the crab group alpha.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"185-192"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/df/87/dr-25-3-185.PMC8670772.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39636610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Constitutive Activating Eel Luteinizing Hormone Receptors Induce Constitutively Signal Transduction and Inactivating Mutants Impair Biological Activity. 组成激活黄体生成素受体诱导组成信号转导，灭活突变体损害生物活性。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2021.25.3.133

Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min

{"title":"Constitutive Activating Eel Luteinizing Hormone Receptors Induce Constitutively Signal Transduction and Inactivating Mutants Impair Biological Activity.","authors":"Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min","doi":"10.12717/DR.2021.25.3.133","DOIUrl":"https://doi.org/10.12717/DR.2021.25.3.133","url":null,"abstract":"In contrast to the human lutropin receptor (hLHR) and rat LHR (rLHR), very few naturally occurring mutants in other mammalian species have been identified. The present study aimed to delineate the mechanism of signal transduction by three constitutively activating mutants (designated M410T, L469R, and D590Y) and two inactivating mutants (D383N and Y546F) of the eel LHR, known to be naturally occurring in human LHR transmembrane domains. The mutants were constructed and measured cyclic adenosine monophosphate (cAMP) accumulation via homogeneous time-resolved fluorescence assays in Chinese hamster ovary (CHO)-K1 cells. The activating mutant cells expressing eel LHR-M410T, L469R, and D590Y exhibited a 4.0-, 19.1-, and 7.8-fold increase in basal cAMP response without agonist treatment, respectively. However, inactivating mutant cells expressing D417N and Y558F did not completely impaired signal transduction. Specifically, signal transduction in the cells expressing activating mutant L469R was not occurred with a further ligand stimulation, showing that the maximal response exhibited approximately 53% of those of wild type receptor. Our results suggested that the constitutively activating mutants of the eel LHR consistently occurred without agonist treatment. These results provide important information of LHR function in fish and regulation with regard to mutations of highly conserved amino acids in glycoprotein hormone receptors.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 3","pages":"133-143"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/5c/dr-25-3-133.PMC8670774.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39621056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Antitumor Effect of Metformin in Combination with Binimetinib on Melanoma Cells. 二甲双胍联合比尼美替尼对黑色素瘤细胞的抗肿瘤作用。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.93

Eunsung Lee, Yongjae Kwon, Jiwon Kim, Deokbae Park, Youngki Lee

{"title":"Antitumor Effect of Metformin in Combination with Binimetinib on Melanoma Cells.","authors":"Eunsung Lee, Yongjae Kwon, Jiwon Kim, Deokbae Park, Youngki Lee","doi":"10.12717/DR.2021.25.2.93","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.93","url":null,"abstract":"Cutaneous melanoma is a fatal disease for patients with distant metastasis. Metformin is the most widely used anti-diabetic drug, and proved to suppress cell proliferation and metastasis in diverse cancers including melanoma. We previously reported that MEK inhibitor trametinib increases the expression of epithelial-mesenchymal transition (EMT) regulators and melanoma cell motility, which are suppressed by addition of metformin in A375 melanoma cells. To confirm our findings further, we first evaluated the effect of metformin in combination with another MEK inhibitor binimetinib on cell viability in G361 melanoma cells. We then investigated whether binimetinib affects the expression of EMT regulators and cell motility. We finally monitored the effect of metformin on binimetinib-induced cell migration. Cell viability assay showed that combination index (CI) value at ED50 is 0.80, suggesting synergy for the combination of metformin with binimetinib. Our results also revealed that binimetinib increased the expression of EMT regulators such as integrin αV, fibronectin and slug, which correlate well with the enhanced cell migration in wound healing assay. Metformin, on the contrary, suppressed the expression of sparc, integrin αV, fibronectin and N-cadherin with the reduced cell motility. The combination treatment showed that metformin counteracts the binimetinib-induced increase of cell motility. Overall, these results suggest that metformin with binimetinib might be useful as a potential therapeutic adjuvant against cell survival and metastatic activity in melanoma patients.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"93-104"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/17/79/dr-25-2-93.PMC8328479.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Geographical Genetic Differences of Arkshell Populations in Korea. 韩国黑壳居群地理遗传差异分析。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.105

Jong-Man Yoon

{"title":"Analysis of Geographical Genetic Differences of Arkshell Populations in Korea.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2021.25.2.105","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.105","url":null,"abstract":"A PCR-founded genetic analysis aim and principle was used to foster a hierarchical polar dendrogram of the Euclidean genetic distances (GDs) for two arkshell populations, Scapharca broughtonii (YEOSU, Yeosu population and JINHAE, Jinhae population). Five oligonucleotides primers were make use of to craft 354 and 390 scorable bands in the Yeosu and Jinhae populations, respectively, outspreading in DNA fragment size from 100 bp to 1,600 bp. The bandsharing (BS) results disclosed that the Jinhae population had a higher average BS value (0.700) than that for the Yeosu population (0.692). The GD between individuals supported an adjacent association in grouping II (JINHAE 12 - JINHAE 22). The observation of a noteworthy GD between the two Scapharca populations verified that this PCR-generated technique could be a profitable attempt for within- and between-population-grounded biological DNA scrutiny. The potential of PCR inquiry will be favorable in the selection of individuals and/or populations for several reproductive- and/or quarantine-connected characters in aquafarming manufacture.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"105-111"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/05/eb/dr-25-2-105.PMC8328478.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Maternal Hypothyroidism on the Pubertal Development in Female Rat Offspring. 母体甲状腺功能减退症对雌性大鼠子代青春期发育的影响。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.83

Jin-Soo Park, Sung-Ho Lee

{"title":"Effects of Maternal Hypothyroidism on the Pubertal Development in Female Rat Offspring.","authors":"Jin-Soo Park, Sung-Ho Lee","doi":"10.12717/DR.2021.25.2.83","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.83","url":null,"abstract":"The present study was performed to investigate the effect of maternal hypothyroidism and puberty onset in female rat pups. To do this, we employed propylthiouracil (PTU) to prepare a hypothyroid rat model. Pregnant rats were treated with PTU (0.025%) in drinking water from gestational day 14 to postnatal day 21 of offspring. Comparison of general indices such as body and tissue weights and puberty indices such as vaginal opening (VO) and tissue histology between control and PTU-treated rats were conducted. There was no significant difference in the date of VO between control and PTU group. The body weights of the PTU group were significantly lower, only 36.8% of the control group (p<0.001). Although the absolute thyroid weight was not changed by PTU treatment, the relative weight increased significantly about 2.8 times (p<0.001), indicating that hypothyroidism was successfully induced. On the other hand, the absolute weights of the ovary and uterus were markedly decreased by PTU administration (p<0.001), and the relative weight was not significantly changed. The ovarian histology of PTU group revealed the advanced state of differentiation (i.e., presence of corpora lutea). Inversely, the uterine histology of PTU group showed underdeveloped structures compared those in control group. Taken together, the present study demonstrates that our maternal hypothyroidism model resulted in minimal effect on pubertal development symbolized by VO despite of huge retardation in somatic growth. More sophisticatedly designed hypothyroidism model will be helpful to achieve a better understanding of pubertal development and related disorders.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"83-91"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/b8/dr-25-2-83.PMC8328481.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation. 赤斑石斑鱼卵母细胞成熟过程中体外性类固醇代谢的研究。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.75

In Joon Hwang, Hea Ja Baek

{"title":"In Vitro Sex Steroid Metabolism in Red Spotted Grouper, Epinephelus akaara during Oocyte Maturation.","authors":"In Joon Hwang, Hea Ja Baek","doi":"10.12717/DR.2021.25.2.75","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.75","url":null,"abstract":"We studied steroid metabolites produced from red-spotted grouper ovarian follicles during maturation. Oocytes with 350-500 μm diameter were in vitro incubated in the presence of [3H] 17α-hydroxyprogesterone as a precursor. Steroid metabolites were extracted from incubated media and oocytes. The extracts were separated and identified using thin layer chromatography, high performance liquid chromatography and gas chromatography-mass spectrometry. The identified metabolites were androstenedione (A4), testosterone (T) and estrone (E1). The metabolites of A4 was dominant in all size of oocytes and it was the highest in 480 μm diameter oocytes. The metabolites of two progestins, 17α,20β-dihydroxy-4-pregnen-3-one and 17α,20α-dihydroxy-4-pregnen-3-one were detected in the oocytes less than 480 μm diameter although they were not identified definitely. In the oocytes of 480 μm diameter, metabolite of progestin was the highest, and germinal vesicle (GV) was still in the middle of cytoplasm. In the oocytes of 500 μm diameter, GV was began to migrate and the major metabolites were A4 and E1. The metabolite of E1 was detected in all size of oocytes and it was higher than that of E2. These results suggest that oocytes of 480 μm diameter are the transitional stage involving steroidogenic shift to final oocyte maturation and potential function of E1 during maturation process.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"75-82"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5f/92/dr-25-2-75.PMC8328477.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Normative Review on Non-Invasive Prenatal Diagnosis (NIPD): Focusing on the German Discussion on PrenaTest®. 无创产前诊断(NIPD)的规范性回顾:以德国对PrenaTest®的讨论为重点。

Development & reproduction Pub Date : 2021-06-01 Epub Date: 2021-06-30 DOI: 10.12717/DR.2021.25.2.113

Na-Kyoung Kim

{"title":"A Normative Review on Non-Invasive Prenatal Diagnosis (NIPD): Focusing on the German Discussion on PrenaTest®.","authors":"Na-Kyoung Kim","doi":"10.12717/DR.2021.25.2.113","DOIUrl":"https://doi.org/10.12717/DR.2021.25.2.113","url":null,"abstract":"This article aims to introduce German discussion on the approval of the non-invasive prenatal diagnosis (NIPD), which started with the development of PrenaTest® by LifeCodexx AG. The discussion started with the concern that the non-invasive nature of NIPD, such as PrenaTest®, may rapidly expand the use and scope of similar tests, thus leading to a new era of eugenics. Based on this concern, the need for clear clinical guidelines on specific indications for NIPD has been suggested. Along the same line, it was discussed whether PrenaTest® is against the Basic Law prohibiting discrimination on grounds of disability and whether the test is outside the scope of the purpose of gene testing limited by Genetic Diagnosis Act. Through such discussion, the Federal Ministry of Health of Germany established the preconditions for inclusion of NIPD in the German public health insurance system. For this, the German motherhood guideline was amended and the information for the insured persons provided to pregnant women was included in the amended guideline. Such discussion made in Germany provides insight on which points should be considered when various gene testings are accepted in Korea , in which genetic communication has not been systematized yet. In particular, German counseling system for pregnant women will provide valuable insights for Korea where the direction for regulations on abortion has not been established even after the ruling by the Constitutional Court that charges for abortion are against the constitution.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 2","pages":"113-121"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/60/dr-25-2-113.PMC8328480.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39307009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sex of Mussel Mytilus coruscus (Bivalvia: Mytilidae) : Sequential Hermaphroditism. 贻贝的性别(双壳目:贻贝科):序贯雌雄同体。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.55

Hyeon Jin Kim, So Ryung Shin, Han Young Oh, Jae Won Kim, Jung Sick Lee

引用次数: 2

Erratum to : PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells. 涂覆玻璃体连接蛋白肽的PVDF纳米纤维支架促进人胚胎干细胞的神经分化。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.73

Byeong-Min Jeon, Gyu-Bum Yeon, Hui-Gwan Goo, Kyung Eun Lee, Dae-Sung Kim

引用次数: 0

Benzo[a]pyrene Cytotoxicity Tolerance in Testicular Sertoli Cells Involves Aryl-hydrocarbon Receptor and Cytochrome P450 1A1 Expression Deficiencies. 睾丸支持细胞对苯并[a]芘的细胞毒性耐受涉及芳烃受体和细胞色素P450 1A1表达缺陷。

Development & reproduction Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.12717/DR.2021.25.1.15

Jin-Tac Kim, Ji-Eun Park, Seung-Jin Lee, Wook-Joon Yu, Hye-Jeong Lee, Jong-Min Kim

{"title":"Benzo[a]pyrene Cytotoxicity Tolerance in Testicular Sertoli Cells Involves Aryl-hydrocarbon Receptor and Cytochrome P450 1A1 Expression Deficiencies.","authors":"Jin-Tac Kim, Ji-Eun Park, Seung-Jin Lee, Wook-Joon Yu, Hye-Jeong Lee, Jong-Min Kim","doi":"10.12717/DR.2021.25.1.15","DOIUrl":"https://doi.org/10.12717/DR.2021.25.1.15","url":null,"abstract":"Benzo[a]pyrene (B[a]P) is a potent carcinogen and is classified as an endocrine-disrupting chemical. In mammalian testes, Sertoli cells support spermatogenesis. Therefore, if these cells are negatively affected by exposure to xenotoxic chemicals, spermatogenesis can be seriously disrupted. In this context, we evaluated whether mouse testicular TM4 Sertoli cells are susceptible to the induction of cytotoxicity-mediated cell death after exposure to B[a] P in vitro. In the present study, while B[a]P and B[a]P-7,8-diol were not able to induce cell death, exposure to BPDE resulted in cell death. BPDE-induced cell death is accompanied by the activation of caspase-3 and caspase-7. Depolarization of the mitochondrial membrane and cytochrome c release from mitochondria were observed in benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE)-treated cells. These results indicate that TM4 cells are susceptible to apoptosis in a caspase-dependent manner. Western blot and reverse transcription-polymerase chain reaction (RT-PCR) analyses showed that aryl hydrocarbon receptor (AhR) expression was almost undetectable in TM4 cells and that its expression was not altered after B[a]P treatment. This indicates that TM4 cells are nearly AhR-deficient. In TM4 cells, the CYP1A1 protein and its activity were not present. From these results, it is clear that AhR may be a prerequisite for CYP1A1 expression in TM4 cells. Therefore, TM4 cells can be referred to as CYP1A1-deficient cells. Thus, TM4 Sertoli cells are believed to have a rigid and protective cellular machinery against genotoxic agents. In conclusion, it is suggested that tolerance to B[a]P cytotoxicity is associated with insufficient AhR and CYP1A1 expression in testicular Sertoli cells.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 1","pages":"15-24"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7f/6c/dr-25-1-15.PMC8087263.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38971268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2