Development & reproduction最新文献_第7页

Lin28 and Imp are Required for Stability of Bowl Transcripts in Hub Cells of the Drosophila Testis. 睾丸果蝇中心细胞碗状转录本的稳定性需要Lin28和Imp。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.313

Van To, Hyun Ju Kim, Wijeong Jang, Perinthottathil Sreejith, Changsoo Kim

引用次数: 0

Effects of Repeated Ovarian Stimulation on Ovarian Function and Aging in Mice. 反复卵巢刺激对小鼠卵巢功能及衰老的影响。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.213

Jihye Whang, Cheyoung Ahn, Soohyun Kim, Eunji Seok, Yunjeong Yang, Goeun Han, Haeun Jo, Hyunwon Yang

{"title":"Effects of Repeated Ovarian Stimulation on Ovarian Function and Aging in Mice.","authors":"Jihye Whang, Cheyoung Ahn, Soohyun Kim, Eunji Seok, Yunjeong Yang, Goeun Han, Haeun Jo, Hyunwon Yang","doi":"10.12717/DR.2021.25.4.213","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.213","url":null,"abstract":"Controlled ovarian hyperstimulation (COH) is routinely used in the in vitro fertilization and embryo transfer (IVF-ET) cycles to increase the number of retrieved mature oocytes. However, the relationship between repeated COH and ovarian function is still controversial. Therefore, we investigated whether repeated ovarian stimulation affects ovarian aging and function, including follicular development, autophagy, and apoptosis in follicles. Ovarian hyperstimulation in mice was induced by intraperitoneal injection with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Mice subjected to ovarian stimulation once were used as a control group and 10 times as an experimental group. Repeated injections with PMSG and hCG significantly reduced the number of primary follicles compared to a single injection. The number of secondary and antral follicles increased slightly, while the number of corpus luteum increased significantly with repeated injections. On the other hand, repeated injections did not affect apoptosis in follicles associated with follicular atresia. The expression of autophagy-related genes Atg5, Atg12, LC3B, and Beclin1, cell proliferation-related genes mTOR, apoptosis-related genes Fas, and FasL was not significantly different between the two groups. In addition, the expression of the aging-related genes Dnmt1, Dnmt3a, and AMH were also not significantly different. In this study, we demonstrated that repeated ovarian stimulation in mice affects follicular development, but not autophagy, apoptosis, aging in ovary. These results suggest that repetition of COH in the IVF-ET cycle may not result in ovarian aging, such as a decrease in ovarian reserve in adult women.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"213-223"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5f/b4/dr-25-4-213.PMC8807135.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Tissue-Specific Interferon Regulatory Factor 3 (IRF3) Gene Expression against Viral Infection in Paralichthys olivaceus. 组织特异性干扰素调节因子3 (IRF3)基因在橄榄鲆抗病毒感染中的表达分析。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.235

Kyung-Hee Kim, Sanghyun Lee, Jong-Won Park, Hyo Sun Jung, Julan Kim, Hyerim Yang, Jeong-Ho Lee, Dain Lee

{"title":"Analysis of Tissue-Specific Interferon Regulatory Factor 3 (IRF3) Gene Expression against Viral Infection in Paralichthys olivaceus.","authors":"Kyung-Hee Kim, Sanghyun Lee, Jong-Won Park, Hyo Sun Jung, Julan Kim, Hyerim Yang, Jeong-Ho Lee, Dain Lee","doi":"10.12717/DR.2021.25.4.235","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.235","url":null,"abstract":"Interferon Regulatory Factor 3 (IRF3) is a member of interferon-regulated transcription factor family and is known to play an important role in the innate immune response against viral infections. In this study, the expression of IRF3 in different tissues, developmental stages, and stocking densities of olive flounder was investigated. The expression of IRF3 was observed to gradually increase in early-stage juvenile fish. The highest expression was observed in later-stage juvenile fish when immune tissues were formed. High IRF3 expression was observed in the muscles and the brain tissues. The expression of IRF3 was observed in fish at different stocking densities after viral hemorrhagic septicemia virus (VHSV) infection. It yielded an interesting expression pattern in the muscles and the brain tissues of fish stocked at low density. These observations can be used as basic data for the study of the expression of immune response-related genes against viruses based on stocking density and immune systems in other fish species.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"235-244"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/be/bd/dr-25-4-235.PMC8807130.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expressional Modulation of Aquaporin 1 and 9 in the Rat Epididymis by an Anabolic-Androgenic Steroid, Nandrolone Decanoate. 合成代谢雄激素类固醇癸酸诺龙对大鼠附睾水通道蛋白1和9表达的调节。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.245

Ki-Ho Lee

{"title":"Expressional Modulation of Aquaporin 1 and 9 in the Rat Epididymis by an Anabolic-Androgenic Steroid, Nandrolone Decanoate.","authors":"Ki-Ho Lee","doi":"10.12717/DR.2021.25.4.245","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.245","url":null,"abstract":"The spermatozoa become mature in the epididymis which is divided into initial segment and caput, corpus, and cauda epididymis. The water movement across the epididymal epithelium is important for creating luminal microenvironment for sperm maturation. Aquaporins (Aqps) are water channel proteins, and expression of Aqps is regulated by androgens. The current research was focused to examine expressional regulation of Aqp1 and Aqp9 by an androgenic-anabolic steroid, nandrolone decanoate (ND). The ND at the low dose (2 mg/ kg body weight/week) or high dose (10 mg) was subcutaneously administrated into male rats for 2 or 12 weeks. Transcript levels of Aqp1 and Aqp9 were determined by quantitative real-time polymerase chain reaction (PCR) analyses. In the initial segment, level of Aqp1 was decreased with 12 week-treatment, while Aqp9 level was decreased by the high dose treatment for 12 weeks. In the caput epididymis, Aqp9 expression was decreased by the low dose treatment. The 2 week-treatment resulted in an increase of Aqp1 level but a decrease of Aqp9 expression in the corpus epididymis. In the corpus epididymis, the 12 week-treatment at the low dose caused the reduction of Aqp1 and Aqp9 levels, but the high dose treatment resulted in an increase of Aqp1 expression and a decrease of Aqp9 level. In the cauda epididymis, Aqp1 expression was decreased by 2 and 12 week-treatments, while increases of Aqp9 levels was detected with the high dose treatment for 2 weeks and with 12 week-treatment. These findings indicate differential regulation of Aqp1 and Aqp9 expression among epididymal segments by ND.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"245-255"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/80/dr-25-4-245.PMC8807133.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Distances for Intra- and Between-Group of Scapharca subcrenata from Yeosu of the Korea. 韩国丽水亚鲫组内和组间的遗传距离。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.305

Jong-Man Yoon

{"title":"Genetic Distances for Intra- and Between-Group of Scapharca subcrenata from Yeosu of the Korea.","authors":"Jong-Man Yoon","doi":"10.12717/DR.2021.25.4.305","DOIUrl":"10.12717/DR.2021.25.4.305","url":null,"abstract":"The oligonucleotides polymers (ON-polymers) were used producing a total of 110 loci unique to each clam population (LUECP) in group one and 132 in group two, respectively, varying in amount of DNA fragments (FRs) from greater than near 50 to a smaller quantity than 1,050 bp. The larger FR amounts (>1,050 bp) are not noticed in the two Scapharca subcrenata groups. The ON-polymer OPD-01 produced 33 LUECP, which were defining each group, almost 300 bp, 450 bp, and 500 bp, in the group one. The OPD-15 recognized 22 loci shared by the two clam populations (Loci shared by the two clam populations, LSTCP), a variety of FRs of sizes 300 bp that were equivalent in all specimens. The mean number of LUECP was varied and 1.2-fold greater in the shellfish group two than in the group one. Respecting mean bandsharing (BS) grade outcomes, entities in the shellfish group one (0.779±0.011) had a little higher BS grades than did entities from the group two (0.756±0.009) (p<0.05). The entities of the shellfish group one are not tightly gathered with other entities of the group two. The genetic distance (GD) (0.422) of this invertebrate (SUBCRENATA 02 and 01) is 7.41-fold hereditarily distinct to the GD (0.057) of the other invertebrate (SUBCRENATA 22 and 19). The polar dendrogram (PDG) procured by the five ON-polymers underlines two characteristic groups.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"305-311"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f7/43/dr-25-4-305.PMC8807124.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39781494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-Surface Loss of Constitutive Activating and Inactivating Mutants of Eel Luteinizing Hormone Receptors. 鳗鱼黄体生成素受体组成型激活和失活突变体的细胞表面损失。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.225

Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min

{"title":"Cell-Surface Loss of Constitutive Activating and Inactivating Mutants of Eel Luteinizing Hormone Receptors.","authors":"Munkhzaya Byambaragchaa, Seung-Hee Choi, Dong-Wan Kim, Kwan-Sik Min","doi":"10.12717/DR.2021.25.4.225","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.225","url":null,"abstract":"The present study aimed to investigate the mechanism of cell surface receptor loss by two constitutively activating mutants (designated L469R, and D590Y) and two inactivating mutants (D417N and Y558F) of the luteinizing hormone receptor (LHR) in the Japanese eel Anguilla japonica, known to naturally occur in human LHR transmembrane domains. We investigated cell surface receptor loss using an enzyme-linked immunosorbent assay in HEK 293 cells. The expression level of wild-type eel LHR was considered to be 100%, and the expression levels of L469R and D417N were 97% and 101%, respectively, whereas the expression levels of D590Y and Y558F slightly increased to approximately 110% and 106%, respectively. The constitutively activating mutants L469R and D590Y exhibited a decrease in cell surface loss in a manner similar to that of wild-type eel LHR. The rates of loss of cell surface agonist-receptor complexes were observed to be very rapid (2.6-6.2 min) in both the wild-type eel LHR and activating mutants. However, cell surface receptor loss in the cells expressing inactivating mutants D417N and Y558F was slightly observed in the cells expressing inactivating mutants D417N and Y558F, despite treatment with a high concentration of agonist. These results provide important information on LHR function in fish and the regulation of mutations of highly conserved amino acids in glycoprotein hormone receptors.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"225-234"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3b/27/dr-25-4-225.PMC8807125.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ovarian Development of Conger Eel in Korea, Conger myriaster, in Captivity. 韩国长鳗鲡卵巢发育，长鳗鲡，人工饲养。

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.269

Se-Un Ki, Chung-Kug Park, Kyoung-Woo Lee, Kyoung-Sik Lee, Joon-Taek Park, Won-Kyo Lee

{"title":"Ovarian Development of Conger Eel in Korea, Conger myriaster, in Captivity.","authors":"Se-Un Ki, Chung-Kug Park, Kyoung-Woo Lee, Kyoung-Sik Lee, Joon-Taek Park, Won-Kyo Lee","doi":"10.12717/DR.2021.25.4.269","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.269","url":null,"abstract":"Effects of water temperature and hormones on ovarian development of conger eel in Korea were investigated. Ovarian development was analyzed by measuring gonadosomatic index (GSI) and oocyte diameter with histological methods. At rearing water temperatures of 12°C, 14°C, and 16°C, GSI value increased from 3.66 at the start of the experiment to 7.44, 8.82, and 7.34 at the end of the experiment, respectively. At rearing water temperatures of 12°C, 14°C, and 16°C, egg diameter increased from 245.11-300.25 µm at the start of the experiment to 377.62-480.27 µm, 396.72-498.54 µm, and 382.29-475.69 µm at the end of the experiment, respectively. Follicular oocyte development revealed that primary yolk globule stage observed from January to March. It entered to secondary yolk globule stage in April and remained at the same stage until July. As a result of examining effects of three hormones (human chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analogue (LHRHa), and salmon pituitary extraction (SPE) on ovarian development, HCG was found to be the most effective one. The progress from diapause of the secondary yolk globule stage to migratory nucleus stage of oocytes could be induced by treating fish with HCG at 1,000 IU/kg. The effect of hormone treatment on ovarian development of conger eel in Korea was the most effective at water temperature of 14°C.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"269-277"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f5/e7/dr-25-4-269.PMC8807132.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor. 马黄体生成素/CG (eLH/CG)受体表达细胞中绒毛膜促性腺激素(eCG)糖基化位点的特异性生物活性

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.199

Munkhzaya Byambaragchaa, Seung-Hee Choi, Hyo-Eun Joo, Sang-Gwon Kim, Yean-Ji Kim, Gyeong-Eun Park, Myung-Hwa Kang, Kwan-Sik Min

{"title":"Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.","authors":"Munkhzaya Byambaragchaa, Seung-Hee Choi, Hyo-Eun Joo, Sang-Gwon Kim, Yean-Ji Kim, Gyeong-Eun Park, Myung-Hwa Kang, Kwan-Sik Min","doi":"10.12717/DR.2021.25.4.199","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.199","url":null,"abstract":"Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species. However, in equidaes, eCG exhibits only LH-like activity. To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant. We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells. We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased. The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG. The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells). Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR. These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"199-211"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/48/dr-25-4-199.PMC8807129.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39767375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ethics and Responsibility of Scientific Researchers in a Pandemic Era. 大流行时代科研人员的伦理与责任

Development & reproduction Pub Date : 2021-12-01 Epub Date: 2021-12-31 DOI: 10.12717/DR.2021.25.4.321

Kyung-Hee Lee

{"title":"Ethics and Responsibility of Scientific Researchers in a Pandemic Era.","authors":"Kyung-Hee Lee","doi":"10.12717/DR.2021.25.4.321","DOIUrl":"https://doi.org/10.12717/DR.2021.25.4.321","url":null,"abstract":"The purpose of this paper is to critique the links between science and scientific researchers, politics and capital surrounding vaccines and vaccination in a pandemic era. It also introduces standards for adapting ethical guidelines for research under public health emergencies to specific circumstances and contexts. It also introduces ethical standards to be applied to scientific research. that is, scientific relevance, social value, cooperative partnerships, reasonable risk-benefit costs, fair and voluntary participation, independent review, and equal moral respect for participants and affected communities. It also outlines the COVID-19 (coronavirus disease 2019) pandemic-consent and other research procedure modifications proposed by Oregon Health and Science University (OHSU). We are on the lookout for powerful capital and hegemonic groups inseparable from politics and important decision makers in the pandemic era - that is, scientists, supporters, and scientific civic groups. It underscores the need for an independent and formal scientific advisory body with the right balance between science and politics.","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"25 4","pages":"321-326"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/05/dr-25-4-321.PMC8807136.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39781496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of Miniaturized Ovaries by In Vitro Culture from Mouse Gonads. 小鼠性腺体外培养制备小型卵巢的研究。

Development & reproduction Pub Date : 2021-09-01 Epub Date: 2021-09-30 DOI: 10.12717/DR.2020.25.3.173

Si Won Jang, Hyun Woo Choi

引用次数: 0