Cyborg and bionic systems (Washington, D.C.)最新文献

Remote Regulation of Molecular Diffusion in Extracellular Space of Parkinson's Disease Rat Model by Subthalamic Nucleus Deep Brain Stimulation. 丘脑下核深部脑刺激对帕金森病大鼠细胞外空间分子扩散的远程调控。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-04-03 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0218

Dan Du, Wanyi Fu, Shaoyi Su, Xin Mao, Liu Yang, Meng Xu, Yi Yuan, Yajuan Gao, Ziyao Geng, Yanjing Chen, Mingming Zhao, Yu Fu, Feng Yin, Hongbin Han

{"title":"Remote Regulation of Molecular Diffusion in Extracellular Space of Parkinson's Disease Rat Model by Subthalamic Nucleus Deep Brain Stimulation.","authors":"Dan Du, Wanyi Fu, Shaoyi Su, Xin Mao, Liu Yang, Meng Xu, Yi Yuan, Yajuan Gao, Ziyao Geng, Yanjing Chen, Mingming Zhao, Yu Fu, Feng Yin, Hongbin Han","doi":"10.34133/cbsystems.0218","DOIUrl":"10.34133/cbsystems.0218","url":null,"abstract":"Subthalamic nucleus deep brain stimulation (STN-DBS) is an effective therapy for Parkinson's disease (PD). However, the therapeutic mechanisms remain incompletely understood, particularly regarding the extracellular space (ECS), a critical microenvironment where molecular diffusion and interstitial fluid (ISF) dynamics are essential for neural function. This study aims to explore the regulatory mechanisms of the ECS in the substantia nigra (SN) of PD rats following STN-DBS. To evaluate whether STN-DBS can modulate ECS diffusion and drainage, we conducted quantitative measurements using a tracer-based magnetic resonance imaging. Our findings indicated that, compared to the PD group, STN-DBS treatment resulted in a decreased diffusion coefficient (D*), shorted half-life (T 1/2), and increased clearance coefficient (k') in the SN. To investigate the mechanisms underlying these changes in molecular diffusion, we employed enzyme-linked immunosorbent assay (ELISA), Western blotting (WB), and microdialysis techniques. The results revealed that STN-DBS led to an increase in hyaluronic acid content, elevated expression of excitatory amino acid transporter 2 (EAAT2), and a reduction in extracellular glutamate concentration. Additionally, to further elucidate the mechanisms influencing ISF drainage, we employed immunofluorescence and immunohistochemical techniques for staining aquaporin-4 (AQP-4) and α-synuclein. The results demonstrated that STN-DBS restored the expression of AQP-4 while decreasing the expression of α-synuclein. In conclusion, our findings suggest that STN-DBS improves PD symptoms by modifying the ECS and enhancing ISF drainage in the SN regions. These results offer new insights into the mechanisms and long-term outcomes of DBS in ECS, paving the way for precision therapies.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0218"},"PeriodicalIF":10.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward Hand Gesture Recognition Using a Channel-Wise Cumulative Spike Train Image-Driven Model. 基于信道累积尖峰序列图像驱动模型的手势识别研究。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-21 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0219

Yang Yu, Zeyu Zhou, Yang Xu, Chen Chen, Weichao Guo, Xinjun Sheng

{"title":"Toward Hand Gesture Recognition Using a Channel-Wise Cumulative Spike Train Image-Driven Model.","authors":"Yang Yu, Zeyu Zhou, Yang Xu, Chen Chen, Weichao Guo, Xinjun Sheng","doi":"10.34133/cbsystems.0219","DOIUrl":"10.34133/cbsystems.0219","url":null,"abstract":"Recognizing hand gestures from neural control signals is essential for natural human-machine interaction, which is extensively applied to prosthesis control and rehabilitation. However, establishing associations between the neural control signals of motor units and gestures remains an open question. Here, we propose a channel-wise cumulative spike train (cw-CST) image-driven model (cwCST-CNN) for hand gesture recognition, leveraging the spatial activation patterns of motor unit firings to distinguish motor intentions. Specifically, the cw-CSTs of motor units were decomposed from high-density surface electromyography using a spatial spike detection algorithm and were further reconstructed into images according to their spatial recording positions. Then, the resultant cwCST-images were fed into a customized convolutional neural network to recognize gestures. Additionally, we conducted an experiment involving 10 gestures and 10 subjects and compared the proposed method with 2 root-mean-square (RMS)-based approaches and a cw-CST-based approach, namely, RMS-image-driven convolutional neural network classification model, RMS feature with linear discrimination analysis classifier, and cw-CST discharge rate feature with linear discrimination analysis classifier. The results demonstrated that cwCST-CNN outperformed the other 3 methods with a higher classification accuracy of 96.92% ± 1.77%. Moreover, analysis of cw-CST and RMS features showed that the former had better separability across gestures and consistency considering training and testing datasets. This study provides a new solution and enhances the accuracy of gesture recognition using neural drive signals in human-machine interaction.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0219"},"PeriodicalIF":10.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Earthworm-Inspired Multimodal Pneumatic Continuous Soft Robot Enhanced by Winding Transmission. 以蚯蚓为灵感的多模态气动连续软机器人。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0204

Jianbin Liu, Pengcheng Li, Zhihan Huang, Haitao Liu, Tian Huang

{"title":"Earthworm-Inspired Multimodal Pneumatic Continuous Soft Robot Enhanced by Winding Transmission.","authors":"Jianbin Liu, Pengcheng Li, Zhihan Huang, Haitao Liu, Tian Huang","doi":"10.34133/cbsystems.0204","DOIUrl":"10.34133/cbsystems.0204","url":null,"abstract":"This paper presents an earthworm-inspired multimodal pneumatic continuous soft robot enhanced by wire-winding transmission. First, a derived overlapped continuous control law based on multiple peristaltic waves is introduced to effectively improve the motion performance of the robot. Second, by applying the wire-winding transmission method, the extension of one segment is simultaneously transformed into the contraction of other segments, achieving coordinated deformation and making it more similar to real earthworms. In addition, an autonomous obstacle-avoidance control strategy based on contact force sensing is developed to enhance the environmental adaptability of the robot. Based on these methods, an earthworm-inspired soft robot that can perform multimodal movements with autonomous obstacle-avoidance ability and enhanced motion efficiency is developed. A series of experiments including in- and cross-plane crawling, obstacle avoidance steering, and pipeline crawling are conducted to validate the robot's multimodal motion capabilities. The robot can achieve a speed of 6.65 mm/s (36.0 × 10-3 bl/s) during in-plane crawling movement and 1.66 mm/s (8.97 × 10-3 bl/s) during pipeline crawling movement. In terms of the in-plane crawling speed, the robot surpasses other robots of the same type. In conclusion, the robot's multimodal capabilities and enhanced motion efficiency demonstrate superior overall performance, and the robot has good potential for medical and industrial applications.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0204"},"PeriodicalIF":10.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Repetitive Mechanical Oscillation Needle-Free Injection through Electrically Induced Microbubbles. 电致微泡重复机械振荡无针注射的研究进展。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0225

Yibo Ma, Wenjing Huang, Naotomo Tottori, Yoko Yamanishi

引用次数: 0

Toward Cyborg: Exploring Long-Term Clinical Outcomes of a Multi-Degree-of-Freedom Myoelectric Prosthetic Hand. 迈向半机械人：探索多自由度肌电假手的长期临床效果。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-18 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0195

Yuki Kuroda, Yusuke Yamanoi, Hai Jiang, Yoshiko Yabuki, Yuki Inoue, Dianchun Bai, Yinlai Jiang, Jinying Zhu, Hiroshi Yokoi

{"title":"Toward Cyborg: Exploring Long-Term Clinical Outcomes of a Multi-Degree-of-Freedom Myoelectric Prosthetic Hand.","authors":"Yuki Kuroda, Yusuke Yamanoi, Hai Jiang, Yoshiko Yabuki, Yuki Inoue, Dianchun Bai, Yinlai Jiang, Jinying Zhu, Hiroshi Yokoi","doi":"10.34133/cbsystems.0195","DOIUrl":"10.34133/cbsystems.0195","url":null,"abstract":"Recent advancements in robotics and sensor technology have facilitated the development of myoelectric prosthetic hands (MPHs) featuring multiple degrees of freedom and heightened functionality, but their practical application has been limited. In response to this situation, formulating a control theory ensuring the hand dexterity of highly functional MPHs has garnered marked attention. Progress in this field has been directed toward employing machine-learning algorithms to process electromyogram patterns, enabling a broad spectrum of hand movements. In particular, the practical application of 5-finger-driven MPHs with such control functions to real users remains limited, and their attributes and challenges have not been thoroughly examined. In this study, we developed a 5-finger MPH equipped with pattern recognition capabilities. Through a long-term clinical trial, encompassing task assessments and subjective evaluations via questionnaires, we explored the MPH's range of applications. The task assessments revealed an expanded range of achievable tasks as the variety of motions increased. However, this enhanced adaptability was paralleled by a decrease in control reliability. Additionally, findings from the questionnaires indicated that enhancements in task performance with MPHs might be more effective in reducing workplace-related disability than in improving activities in everyday life. This study offers valuable insights into the long-term clinical prospects and constraints associated with multi-degree-of-freedom MPHs incorporating pattern recognition functionality.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0195"},"PeriodicalIF":10.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic Shaftless Propeller Millirobot with Multimodal Motion for Small-Scale Fluidic Manipulation. 基于多模态运动的磁力无轴螺旋桨微机器人。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-12 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0235

Yaozhen Hou, Shihao Zhong, Zhiqiang Zheng, Jiabao Du, Ruhao Nie, Qing Shi, Qiang Huang, Huaping Wang

{"title":"Magnetic Shaftless Propeller Millirobot with Multimodal Motion for Small-Scale Fluidic Manipulation.","authors":"Yaozhen Hou, Shihao Zhong, Zhiqiang Zheng, Jiabao Du, Ruhao Nie, Qing Shi, Qiang Huang, Huaping Wang","doi":"10.34133/cbsystems.0235","DOIUrl":"10.34133/cbsystems.0235","url":null,"abstract":"Magnetic miniature robots have shown great potential in biomedical applications in recent years. However, a challenge remains in which it is difficult for magnetic miniature robots to achieve balanced capabilities for multimodal locomotion and fluidic manipulation in various environments. Here, we report a magnetic shaftless propeller-like millirobot (MSPM) that possesses the capabilities of rotating-based multimodal 3-dimensional motion and cargo transportation with untethered manipulation. The MSPM utilizes the propulsion and pumping capabilities of the propeller structure to achieve fluidic manipulation. The shaftless propeller structures are designed to achieve omnidirectional locomotion through rolling, propelling, and tumbling. Additionally, the shaftless 3-blade propeller is used to perform a pumping function to achieve controllable transportation of fluids and particles. We anticipate that the MSPM holds great potential as a minimally invasive device for thrombosis treatment and targeted medicine delivery.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0235"},"PeriodicalIF":10.5,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11903028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression. 超声定位显微镜在评估2型糖尿病进展中的应用。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0117

Tao Zhang, Jipeng Yan, Xinhuan Zhou, Bihan Wu, Chao Zhang, Mengxing Tang, Pintong Huang

{"title":"Application of Ultrasound Localization Microscopy in Evaluating the Type 2 Diabetes Progression.","authors":"Tao Zhang, Jipeng Yan, Xinhuan Zhou, Bihan Wu, Chao Zhang, Mengxing Tang, Pintong Huang","doi":"10.34133/cbsystems.0117","DOIUrl":"10.34133/cbsystems.0117","url":null,"abstract":"Type 2 diabetes is considered as a chronic inflammatory disease in which the dense microvasculature reorganizes with disease progression and is highly correlated with β cell mass and islet function. In this study, we constructed rat models of type 2 diabetes and used ultrasound localization microscopy (ULM) imaging to noninvasively map the pancreatic microvasculature at microscopy resolution in vivo to reflect β cell loss and islet function deterioration, and evaluate the efficacy after anti-cytokine immunotherapy. It was unveiled that ULM morphological and hemodynamic parameters have a strong link with β cell loss and deterioration of pancreatic islet function. This correlation aligns with the observed pathological alterations in the microvessels of islet and demonstrated that ULM can effectively mirror the functionality of β cells during rapid fluctuations in blood glucose levels by observing changes in mean velocity. Furthermore, it was revealed that treatment with anti-cytokine immunotherapy enhances the function and health of β cells by restoring the microvascular environment. Remarkable improvements in vessel morphology (measured by fractal dimension) and hemodynamics (indicated by mean velocity and vessel density) were noted following the anti-cytokine immunotherapy, signifying a significant enhancement at the treatment's conclusion (P < 0.05). These observations suggested that ULM technology holds promise as a visible and efficient tool for monitoring the effectiveness of anti-cytokine immunotherapy in managing type 2 diabetes. Pancreatic microvessel-based ULM may serve as a novel noninvasive method to assess β cells, providing a valuable clinical tool for tracking the progression of type 2 diabetes.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0117"},"PeriodicalIF":10.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Optimization of Microfluidic Chips for Enhancing Droplet Manipulation and Observation via Electrodynamics Simulation. 微流控芯片的结构优化及电动力学模拟研究。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-06 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0217

Yanfeng Zhao, Zhiqiang Zheng, Jiaxin Liu, Xinyi Dong, Haotian Yang, Anping Wu, Qing Shi, Huaping Wang

{"title":"Structural Optimization of Microfluidic Chips for Enhancing Droplet Manipulation and Observation via Electrodynamics Simulation.","authors":"Yanfeng Zhao, Zhiqiang Zheng, Jiaxin Liu, Xinyi Dong, Haotian Yang, Anping Wu, Qing Shi, Huaping Wang","doi":"10.34133/cbsystems.0217","DOIUrl":"10.34133/cbsystems.0217","url":null,"abstract":"Digital microfluidic chips (DMCs) have shown huge potential for biochemical analysis applications due to their excellent droplet manipulation capabilities. The driving force is a critical factor for characterizing and optimizing the performance of droplet manipulation. Conducting numerical analysis of the driving force is essential for DMC design, as it helps optimize the structural parameters. Despite advances in numerical analysis, evaluating driving forces in partially filled electrodes remains challenging. Here, we propose a versatile electrodynamics simulation model designed to analyze the driving forces of partially filled electrodes to optimize the structural parameters of DMCs. This model utilizes finite element analysis to determine the voltage distribution within the DMC and calculates the driving force acting on the droplets using the principles of virtual work. Using this electrodynamics simulation model, we evaluated the effects of various structural parameters, including the dielectric constant and thickness of the dielectric layer, the dielectric constant and conductivity of the droplet, and substrate spacing, on the droplet driving force. This evaluation helps to optimize the structural parameters and enhances the droplet manipulation of DMCs. Measurements of droplet acceleration demonstrated that the droplet acceleration on the partially filled electrode aligns with the simulated driving force trend, which verified the effectiveness of the proposed electrodynamics simulation model. We anticipate that the electrodynamics simulation model is capable of evaluating the driving force in partially filled electrodes within complex DMCs, offering unprecedented possibilities for future structural designs of DMCs.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0217"},"PeriodicalIF":10.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11884587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platinum Wire-Embedded Culturing Device for Interior Signal Recording from Lollipop-Shaped Neural Spheroids. 用于棒棒糖形神经球体内部信号记录的铂丝嵌入式培养装置。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-03-05 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0220

Hongyong Zhang, Nan Huang, Sumin Bian, Mohamad Sawan

{"title":"Platinum Wire-Embedded Culturing Device for Interior Signal Recording from Lollipop-Shaped Neural Spheroids.","authors":"Hongyong Zhang, Nan Huang, Sumin Bian, Mohamad Sawan","doi":"10.34133/cbsystems.0220","DOIUrl":"10.34133/cbsystems.0220","url":null,"abstract":"Three-dimensional (3D) neural cultures are increasingly recognized for their complexity and resemblance to in vivo neural microenvironments. In this paper, we present a novel 3D cell culturing and noninvasive characterization technique of neural spheroids. Based on embedded platinum wires, the cultured cells are lollipop-shaped spheroids where axons are extended and integrated around the embedded wires. Electrical microstimulation enhanced the connectivity between spheroids and demonstrated signal propagation among them. The resultant axonal elongation facilitated the formation of robust neural tracts interconnecting the neural spheroids. Variation of cells' density allows to adjust the spheroid's diameter, identifying 1 million cells as good number of cells for robust spheroid formation. Recordings of spheroid activities reveal higher-quality neural signal measurement from interior cells compared to those obtained from exterior cells. Viability assays confirmed the efficacy of the proposed culturing technique for sustained growth of neural spheroids over a 1-month period. The proposed spheroid culturing technique holds potential applications in various fields, such as development of brain organoids, which enables real-time interconnection characterization and sensing of environment conditions.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0220"},"PeriodicalIF":10.5,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Vascularized Multilayer Chip Reveals Shear Stress-Induced Angiogenesis in Diverse Fluid Conditions. 血管化多层芯片揭示不同流体条件下剪切应力诱导的血管生成。

IF 10.5

Cyborg and bionic systems (Washington, D.C.) Pub Date : 2025-02-28 eCollection Date: 2025-01-01 DOI: 10.34133/cbsystems.0207

Tao Yue, Huiying Yang, Yue Wang, Ning Jiang, Hongze Yin, Xiaoqi Lu, Na Liu, Yichun Xu

{"title":"A Vascularized Multilayer Chip Reveals Shear Stress-Induced Angiogenesis in Diverse Fluid Conditions.","authors":"Tao Yue, Huiying Yang, Yue Wang, Ning Jiang, Hongze Yin, Xiaoqi Lu, Na Liu, Yichun Xu","doi":"10.34133/cbsystems.0207","DOIUrl":"https://doi.org/10.34133/cbsystems.0207","url":null,"abstract":"Tissues larger than 400 μm in size lacking microvascular networks cannot survive for long periods of time in vitro. The development of microfluidic technology provides an efficient research tool for constructing microvascular models in vitro. However, traditional single-layer microfluidic chips faced the limitation of spatial layout and could not provide diverse fluidic environments within a single chip. In this paper, we present a novel microfluidic chip design with a 3-layer configuration that utilizes a polycarbonate (PC) porous membrane to separate the culture fluid channels from the tissue chambers, featuring flexibly designable multitissue chambers. PC porous membranes act as the capillary in the vertical direction, enabling precise hydrogel patterning and successfully constructing a microfluidic environment suitable for microvascular tissue growth. The chip demonstrates the ability to build microvascular networks of different shapes such as triangle, rectangle, and inverted triangle on a single chip for more than 10 days. The microvascular networks cultured for 12 days were successfully perfused with 70-kDa fluorescein isothiocyanate, which indicated that the generated networks had good barrier properties. A correlation between tissue chamber shape and shear stress was demonstrated using COMSOL, and a preliminary validation of the flow direction of interstitial flow and the important effect of shear stress on microvascular growth was demonstrated by vascularization experiments. This flexible and scalable design is ideal for culturing multiple vascularized organ tissues on a single microfluidic chip, as well as for studying the effects of different fluidic factors on microvascular growth.","PeriodicalId":72764,"journal":{"name":"Cyborg and bionic systems (Washington, D.C.)","volume":"6 ","pages":"0207"},"PeriodicalIF":10.5,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11870090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143544793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0