Brazilian journal of veterinary medicine最新文献

{"title":"Survey on sanitary practices and knowledge about infectious diseases among equine owners in the State of Rio Grande do Norte, Brazil.","authors":"Paulo Roberto Medeiros, Liliany Silva Figueiredo, Ubiratan Pereira de Melo, Amanda Louíse Bittencourt Mariz, Emilson Lima de Brito, Ingrid Raquel Dos Santos Araújo, Allyson Lincoln Da Costa Silva, Mariana Henrique da Silveira Costa, Cintia Ferreira, Daniel Barbosa Assis, Camilla Raylly Miguel da Silva, Aldemir Lopes de Souza, Magna Pereira da Silva de Souza","doi":"10.29374/2527-2179.bjvm003323","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm003323","url":null,"abstract":"<p><p>As the primary decision-maker about the health, nutrition, and well-being of their horses, owners' knowledge of correct management practices and clinical changes can potentially affect the immediate health of their horses, in addition to having an impact on the prevention of disease spread in the herd. The adoption of management practices to prevent the introduction and spread of pathogens depends on various factors, including demographics, awareness of the problem, perceived responsibility, previously held beliefs, and sociocultural norms. This study aimed to evaluate the health management practices and the level of knowledge about infectious diseases of equine owners in the state of Rio Grande do Norte, Brazil. A cross-sectional study was conducted by distributing a questionnaire to horse owners in Rio Grande do Norte, Brazil. The participants included horse owners irrespective of the duration of ownership, experience, or sports practiced. In addition to the hygiene and management of animals, the questionnaire addressed topics related to the facilities where the horses were kept. Three hundred and two horse owners distributed in 60 of the 167 municipalities in the state of Rio Grande do Norte participated in this study. Among the interviewed owners, 63.90% (193/302) answered that they do not quarantine animals introduced into the herd or those visiting the property. Regarding the deworming program, 95.03% (287/302) of the owners regularly dewormed their animals. However, more than half of the participants sought veterinary guidance (54.30%). Investing in educational programs and increasing the awareness of equine owners in the state of Rio Grande do Norte about the main infectious diseases in horses is essential toward improving sanitary management and the general health of animals. Informed owners play an essential role in creating safer environments for their horses, thereby contributing to the sustainability of the equine industry.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e003323"},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10712268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of <i>Dirofilaria immitis</i> infection in blood donor dogs of the Rio de Janeiro state.","authors":"Genilson Pereira Gonçalves,&nbsp;Suzane Gallardo Xavier,&nbsp;Nathália da Conceição Lima,&nbsp;Alexandre José Rodrigues Bendas","doi":"10.29374/2527-2179.bjvm002223","DOIUrl":"10.29374/2527-2179.bjvm002223","url":null,"abstract":"<p><p>Dirofilariasis, a parasitic disease caused by the nematode <i>Dirofilaria immitis</i>, commonly known as heartworm, primarily inhabits the pulmonary artery and right heart of dogs and other animals. The disease is transmitted through diptera, predominantly from the <i>Culex</i>, <i>Aedes</i>, and <i>Anopheles</i> genera. Dirofilariasis is cosmopolitan in nature, endemic in coastal regions and tropical climates. Factors such as temperature, humidity, vector density, and the presence of definitive hosts significantly contribute to the spread of this parasitic disease. In the state of Rio de Janeiro, a prevalence of 58.6% of <i>D. immitis</i> infected animals has been recorded in municipalities like Niterói. Given that blood transfusions are routine clinical procedures and blood bags are not always accurately evaluated, an investigation into <i>D. immitis</i> infection in blood donor dogs from the metropolitan region of Rio de Janeiro was conducted. A total of 1044 blood donor dog files from a blood bank in Niterói, RJ, collected from January 2019 to December 2022, were analyzed. These samples, originating from kennels in various municipalities in the Metropolitan Region, were tested for the presence of microfilariae through direct examination using tubes and microhematocrit evaluated in optical microscopy. Additionally, the search for antigens was conducted using the enzyme-linked immunosorbent assay technique. Out of the 1044 records evaluated, 17.8% (186/1044) were positive for heartworm infection, with 2% (21/1044) samples positive for microfilariae and 14.8% (154/1044) positive for <i>D. immitis</i> antigens. The high prevalence rate indicates that canine <i>D. immitis</i> infection remains prevalent in the state of Rio de Janeiro, necessitating effective guidelines for prescribing preventive medications by veterinarians and an increase in epidemiological surveillance in the region.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e002223"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10588812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular analysis of canine circovirus in dogs from animal shelters in Belém, Pará, northern Brazil: first detection at the amazon region.","authors":"Bruna Trindade Moreira Cardoso,&nbsp;Danielle Rodrigues de Deus,&nbsp;Edivaldo Costa Sousa,&nbsp;Kenny da Costa Pinheiro,&nbsp;Jonaia Novaes da Costa,&nbsp;Marcelino Antonio Costa Maués,&nbsp;Márcia Janete de Fátima Mesquita,&nbsp;Dielle Monteiro Teixeira,&nbsp;Jones Anderson Monteiro Siqueira,&nbsp;Hugo Reis Resque,&nbsp;Yvone Benchimol Gabbay,&nbsp;Luciana Damascena da Silva","doi":"10.29374/2527-2179.bjvm000723","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm000723","url":null,"abstract":"<p><p>The canine circovirus (CanCV) is a single-stranded DNA virus that has become an important emerging virus associated with gastroenteritis in dogs worldwide. In the present study, the CanCV was detected by PCR in 15% (22/147) of dogs from animal shelters in Belém, between 2019 and 2020. We observed an association between the CanCV infection and the presence of diarrhea in animals younger than one year of age (p > 0.01). The Brazilian strains were grouped in Chinese genotypes, with 99.54 to 100% nucleotilde homology. The GMRF Bayesian Skyride used the molecular clock model, which was the best suited technique to plot the dataset. The most recent common ancestor (TMRCA) was estimated in 2017, with the evolution rate of 1.6 x 10^-3 s/s/y. The viral family diversity was also investigated, with emphasis on the families of the enteric pathogenic viruses Parvoviridae, Picornaviridae and Astroviridae, which were detected in the CanCV positive pooled samples. This study highlights the importance of the CanCV as an emergent virus that causes diarrhea in Brazilian dogs. The results found herein contribute to the understanding of the role of CanCV in enteric diseases and in the evolutionary molecular characterization of the circulating genotypes. Furthermore, we increased the understanding of the fecal virome in dogs with diarrhea, providing data for the monitoring and prevention viral gastroenteric diseases in domestic animals.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e000723"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary omentum extraskeletal osteosarcoma in a dog: case report.","authors":"Caroline Tessmer Elias Soares,&nbsp;Fernanda Peres Medeiros,&nbsp;Raul Martins","doi":"10.29374/2527-2179.bjvm000423","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm000423","url":null,"abstract":"<p><p>A rescued male mixed-breed dog, approximately nine years old, was evaluated due to progressive weight loss and an enlarged abdomen. An ultrasound revealed a large, indeterminate mass with mineral-like margins visible on the radiographs. The animal underwent an exploratory laparotomy, and the mass was excised. Histopathological analysis revealed characteristics consistent with a primary omentum extraskeletal osteosarcoma. This rare neoplasm, originating from mesenchymal cell proliferation and bone matrix production, is highly malignant. It often results in death due to metastasis and local recurrence or necessitates euthanasia post-diagnosis in certain cases.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e000423"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effect of <i>Heterorhabditis bacteriophora</i> (HP88) on <i>Stomoxys calcitrans</i> (Linnaeus, 1758) larvae (Diptera: Muscidae) in sugarcane bagasse ash.","authors":"Américo de Castro Monteiro,&nbsp;Ana Caroline Ferreira de Souza,&nbsp;Danielle Pereira da Silva,&nbsp;Graziele Calixto Souza,&nbsp;Isadora Luiza Alves Costa,&nbsp;João Luiz Lopes Monteiro,&nbsp;Melissa Carvalho Machado do Couto Chambarelli,&nbsp;Avelino José Bittencourt","doi":"10.29374/2527-2179.bjvm002123","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm002123","url":null,"abstract":"<p><p>The purpose of this study was to evaluate the effect of the EPN <i>Heterorhabditis bacteriophora</i> HP88 on <i>Stmoxys. calcitrans</i> larvae in sugarcane bagasse ash. Groups of 10 stable fly larvae were placed in Petri dishes containing filter paper and bagasse ash. Concentrations of 50, 150 and 250 EPNs/larva of <i>S. calcitrans</i> in four milliliters of distilled water were added to each plate. In the control group contained only distilled water, without EPNs. The bioassay had three replications and was maintained at 27 ± 1°C and 70-80% relative humidity. It was observed that mortality rate in all treated groups was significantly higher than in the control group (26,6%). The mortality rate in the presence of 50 EPNs/larva (46,6%) was lower than in 150 EPNs/larva (76,3%), which in turn was lower than 250 EPNs/larva group (93,3%). It was verified by analysis of variance and regression that there was a linear pattern of mortality, that is, the higher the EPNs/larva concentration, the higher the larval mortality. It was concluded that EPN <i>H. bacteriophora</i> HP88 was capable of infecting and causing mortality of stable fly larvae in sugarcane bagasse ash.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e002123"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibrosis in canine transmissible venereal tumor after chemotherapy with vincristine.","authors":"Anderson do Prado Duzanski,&nbsp;Haline Ballestero Feo,&nbsp;Luis Mauricio Montoya Flórez,&nbsp;Fernando Carmona Dinau,&nbsp;Bruna Ribeiro Paiva,&nbsp;Cláudia Valéria Seullner Brandão,&nbsp;Noeme Sousa Rocha","doi":"10.29374/2527-2179.bjvm000123","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm000123","url":null,"abstract":"<p><p>The canine transmissible venereal tumor is type of transmissible cancer that occurs naturally through allogenic cellular transplants. Commonly diagnosed in the genital area of sexually active dogs, the tumor typically responds well to vincristine sulfate chemotherapy, although there are cases of resistance to the drug correlated with the tumoral phenotype. We describe herein a case of fibrosis in an area affected by the tumor in a dog after vincristine chemotherapeutic treatment that was associated with an idiosyncratic reaction to the drug.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e000123"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9424575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of parasitism by <i>Eutrichophilus cercolabes</i> (Phthiraptera: Trichodectidae) on <i>Coendou spinosus</i> (Erethizontidae) in Rio Grande do Sul, Brazil - case report.","authors":"Julia Somavilla Lignon,&nbsp;Diego Moscarelli Pinto,&nbsp;Rafael Almeida Fighera,&nbsp;Silvia Gonzalez Monteiro","doi":"10.29374/2527-2179.bjvm000823","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm000823","url":null,"abstract":"<p><p><i>Coendou spinosus</i> is a species of rodent popularly known as porcupine, it has a great ability to adapt to different habitats and is found in tropical forests in countries such as Bolivia, Brazil, Venezuela and Guianas. This mammal has already been identified as a reservoir of several pathogenic agents for humans and other animals and has a variety of ectoparasites, endoparasites and hemoparasites little studied and described. Due to this, the objective was to report the parasitism by <i>Eutrichophilus cercolabes</i> in <i>C. spinosus</i> in the central region of Rio Grande do Sul, southern Brazil. In total, 16 lice were found, one male and 15 females of the species. This is the first report of the parasitism of this Phthiraptera on <i>C. spinosus</i> in Rio Grande do Sul. The scarcity of reports on the taxonomy and biotic characteristics, as well as the vector capacity of pathogens of most species of ectoparasites of wild animals, highlights the need for further studies on the distribution of these arthropods in different regions and host species.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e000823"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10461639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10117812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eurytrematosis in cattle in southern Espírito Santo State, Brazil - case report.","authors":"Renata de Paula Santos,&nbsp;Caio Alves Cardoso,&nbsp;Marcos Paulo Brinati Miranda,&nbsp;Eduardo Vargas de Oliveira,&nbsp;Júlio Francisco Valiati Marin,&nbsp;Natânia do Carmo Sperandio,&nbsp;Louisiane de Carvalho Nunes,&nbsp;Isabella Vilhena Freire Martins","doi":"10.29374/2527-2179.bjvm002023","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm002023","url":null,"abstract":"<p><p>This work reports an outbreak of eurytrematosis in cattle in the municipality of Ibitirama, southern Espírito Santo State, Brazil. Six cattle were necropsied from August to December 2019, with finding of <i>Eurytrema coelomaticum</i> in the pancreas. A survey of epidemiological data was carried out on the farms along with coproparasitological examination of cattle from the same herd. Parasites were found in all necropsied animals, with different degrees of parasitism, ranging from mild to massive infection (6 - 2000 specimens). Macroscopic analyses of the pancreas revealed changes in 83.33% (5/6) of the cases, and by microscopy, pancreatic fibrosis ranging from Grade I to Grade III was observed. Inspection of the grazing areas confirmed the presence of two intermediate hosts, a terrestrial snail of the <i>Bradybaena</i> genus, with larval forms of the trematode in histological findings, and a grasshopper of the <i>Conocephalus</i> genus. Although none of the cattle showed clinical signs in the coproparasitological examination, 73.80% (31/42) tested positive for <i>E. coelomaticum</i> eggs. This is the first record of an outbreak of eurytrematosis in cattle in Espírito Santo State, indicating the importance of carrying out diagnosis based on epidemiology and necroscopic and parasitological examinations in animals in the region so that appropriate control measures can be adopted.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e00223"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10241031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Refrigerated modified Knott concentrate enables long-term morphological viability of canine blood microfilariae.","authors":"Leticia Gomes Zanfagnini,&nbsp;Tatiane Paula da Silva,&nbsp;Diefrey Ribeiro Campos,&nbsp;Soraia Figueiredo de Souza,&nbsp;Patrícia Fernandes Nunes da Silva Malavazi,&nbsp;Rômulo Silva de Oliveira,&nbsp;Cíntia Daudt,&nbsp;Acácio Duarte Pacheco","doi":"10.29374/2527-2179.bjvm000223","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm000223","url":null,"abstract":"<p><p>There are several methods of laboratory diagnosis of filarids, the most used are the thick smear and the Knott method. Both are quick to perform, have a low cost and allow observing the presence, quantifying and analyzing the morphological characteristics of microfilariae. Knowing the morphological viability of fixed microfilariae is of practical importance, as it allows the transport of samples to a laboratory, facilitates epidemiological studies , and allows the storage of samples for didactic. Thus, the aim of this study was to evaluate the morphological viability of microfilariae fixed in the refrigerated modified knott test using 2% formalin. To perform the modified Knott technique, 10 samples of microfilaremic dogs aged over 6 months were used. To evaluate the morphological viability time of the microfilariae in the modified Knott concentrate, the evaluations were repeated after intervals of 0, 1, 7, 30, 60, 120, 180, 240, and 304 days. In the present study, we did not verify any difference in the morphology of the microfilariae in any of the analyzed intervals from day 0 to 304 days, and it is possible to conclude that the use of 2% formalin in the modified Knott technique allows the microfilariae to be identified in a period of 304 days. days after processing the sample without changes in its morphology.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e000223"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10153453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9424574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the susceptibility to fipronil of <i>Rhipicephalus microplus</i> larvae from egg masses incubated at different times of oviposition.","authors":"Daniela de Oliveira Rocha,&nbsp;Roxanne Marina da Silva Roque,&nbsp;Thiago de Souza Vieira,&nbsp;Ingrid Lins Raquel de Jesus,&nbsp;Brena Gava Guimarães,&nbsp;Marisa Beatriz da Silva Rocha,&nbsp;Fabio Barbour Scott,&nbsp;Barbara Rauta Avelar","doi":"10.29374/2527-2179.bjvm005922","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm005922","url":null,"abstract":"<p><p>The objective of this work was to evaluate the susceptibility of <i>R. microplus</i> larvae from different oviposition times to fipronil. The LPT was performed in sextuplicate, at concentrations of 18.75, 37.5, 75, 150 and 300 µg.mL^-1. The LC₅₀ found for the egg masses incubated with +7, +14 and +21 days were respectively 105.87, 110.71 and 121.22 µg.mL^-1. The larvae originating from egg masses from the same group of engorged females, incubated on different days, presented similar mortality rates compared to the evaluated fipronil concentrations, facilitating the maintenance of laboratory colonies of this tick species.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e005922"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10079253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9282877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}