Biomolecules & biomedicine最新文献_第7页

The association of plasma homocysteine levels with short-term mortality in sepsis patients: A meta-analysis. 血浆同型半胱氨酸水平与败血症患者短期死亡率的关系：荟萃分析

Biomolecules & biomedicine Pub Date : 2024-10-29 DOI: 10.17305/bb.2024.11259

Xinxing Lu, Xueyan Yuan, Yali Chao, Xiao Wu, Airan Liu

{"title":"The association of plasma homocysteine levels with short-term mortality in sepsis patients: A meta-analysis.","authors":"Xinxing Lu, Xueyan Yuan, Yali Chao, Xiao Wu, Airan Liu","doi":"10.17305/bb.2024.11259","DOIUrl":"https://doi.org/10.17305/bb.2024.11259","url":null,"abstract":"The association between plasma homocysteine (Hcy) levels and short-term mortality in sepsis patients remains unclear. This meta-analysis aimed to clarify this potential relationship. Following PRISMA 2020 and Cochrane Handbook guidelines, we conducted a comprehensive literature search in the PubMed, Embase, and Web of Science databases up to June 24, 2024. We included cohort studies that assessed the association between plasma Hcy levels and all-cause mortality in adult sepsis patients. Standardized mean differences (SMDs) and odds ratios (ORs) with 95% confidence intervals (CIs) were calculated using a random-effects model to account for potential heterogeneity. Nine cohort studies involving 771 sepsis patients were included. Overall, no significant difference in plasma Hcy levels was observed between survivors and non-survivors (SMD: -0.23, 95% CI: -0.84 to 0.37, P = 0.45), with substantial heterogeneity (I² = 86%). Subgroup analysis revealed lower plasma Hcy levels among survivors in Chinese patients (SMD: -1.56, 95% CI: -1.98 to -1.13, P < 0.001) but not in non-Asian patients. Plasma Hcy levels were not significantly associated with all-cause mortality (OR per 1-unit increment: 1.03, 95% CI: 0.95 to 1.11, P = 0.51), with notable heterogeneity (I² = 72%). However, a significant association was found in Chinese patients (OR: 1.09, 95% CI: 1.03 to 1.15, P = 0.003), but not in non-Asian patients. In conclusion, plasma Hcy levels were not generally associated with short-term mortality in sepsis patients. However, significant associations were observed in Chinese patients, suggesting potential ethnic differences that warrant further investigation.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NEWS, SIRS and qSOFA criteria for predicting sepsis and sepsis with high risk of death in emergency room: A comparison study and improved predictive models based on local data from CETAT and MIMIC-IV databases. NEWS、SIRS 和 qSOFA 标准用于预测急诊室脓毒症和高死亡风险脓毒症：基于 CETAT 和 MIMIC-IV 数据库本地数据的比较研究和改进预测模型。

Biomolecules & biomedicine Pub Date : 2024-10-27 DOI: 10.17305/bb.2024.11134

Wenwen Wang, Kaipeng Wang, Yueguo Wang, Qingyuan Liu, Jian Sun, Ronghua Shi, Sicheng Liu, Huanli Wang, Yuan Yuan, Jun Xu, Kui Jin, Yixin Zhang

{"title":"NEWS, SIRS and qSOFA criteria for predicting sepsis and sepsis with high risk of death in emergency room: A comparison study and improved predictive models based on local data from CETAT and MIMIC-IV databases.","authors":"Wenwen Wang, Kaipeng Wang, Yueguo Wang, Qingyuan Liu, Jian Sun, Ronghua Shi, Sicheng Liu, Huanli Wang, Yuan Yuan, Jun Xu, Kui Jin, Yixin Zhang","doi":"10.17305/bb.2024.11134","DOIUrl":"10.17305/bb.2024.11134","url":null,"abstract":"Early identification of sepsis in emergency department patients is critical for initiating timely interventions, highlighting the need for effective predictive scoring systems. A retrospective observational study was conducted using data from the CETAT database collected between December 2019 and October 2021. The study evaluated how well the systemic inflammatory response syndrome (SIRS), quick Sepsis-related Organ Failure Assessment (qSOFA), and National Early Warning Score (NEWS) scoring systems, along with logistic regression models, predict sepsis, and high-risk sepsis in emergency department patients. The logistic regression models were further optimized by incorporating additional features based on local data. A total of 12,799 patients were analyzed, including 1360 sepsis cases, of which 373 were classified as high-risk sepsis. The NEWS score demonstrated superior predictive performance compared to qSOFA and SIRS, with an area under the receiver operating characteristic curve (AUC-ROC) of 0.737 (95% confidence interval [CI] 0.72–0.75) for sepsis and 0.653 (95% CI 0.62–0.69) for high risk sepsis . After optimization, the NEWS-based model improved to an AUC-ROC of 0.756 (95% CI 0.74–0.77) for sepsis and 0.718 (95% CI 0.69–0.75) for high-risk sepsis. Further enhancement was observed with the inclusion of additional clinical variables, resulting in AUC-ROC values of 0.834 (95% CI 0.82–0.85) for sepsis and 0.756 (95% CI 0.73–0.78) for high-risk sepsis. Data from the Medical Information Mart for Intensive Care (MIMIC)-IV database, which included sepsis status and relevant variables for SIRS, qSOFA, and NEWS score calculations, confirmed that the optimized NEWS-based model improved the sepsis prediction AUC-ROC from 0.690 (95% CI 0.68–0.70) to 0.708 (95% CI 0.70–0.72), and consistently outperformed qSOFA and SIRS in sepsis prediction.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALKBH5 modulates m6A modification to enhance acute myeloid leukemia resistance to adriamycin. ALKBH5 可调节 m6A 修饰，从而增强急性髓性白血病对阿霉素的耐药性。

Biomolecules & biomedicine Pub Date : 2024-10-27 DOI: 10.17305/bb.2024.11076

Yonghua Liu, Jinhong Jiang, Yuxiao Zeng, Yu Jiang

{"title":"ALKBH5 modulates m6A modification to enhance acute myeloid leukemia resistance to adriamycin.","authors":"Yonghua Liu, Jinhong Jiang, Yuxiao Zeng, Yu Jiang","doi":"10.17305/bb.2024.11076","DOIUrl":"10.17305/bb.2024.11076","url":null,"abstract":"Acute myeloid leukemia (AML) is a fatal malignancy with rising incidence and low cure rates. This study aims to investigate the effect of alkB homolog 5 (ALKBH5)-mediated N6-methyladenosine (m6A) modification on adriamycin (ADR) resistance in AML. First, the levels of ALKBH5, taurine upregulated 1 (TUG1), YTH N6-methyladenosine RNA binding protein F2 (YTHDF2), euchromatic histone lysine methyltransferase 2 (EHMT2), and SH3 domain-binding glutamate-rich protein-like (SH3BGRL) were measured. IC50 values, cell proliferation, and apoptosis were determined. m6A levels were analyzed, and the binding interactions between TUG1 and YTHDF2, as well as TUG1 and EHMT2, were assessed. The stability of TUG1 and the enrichment of EHMT2 and H3K9me2 on the SH3BGRL promoter were confirmed. In vivo experiments were conducted to further validate the results. The findings revealed that ALKBH5 was overexpressed in both AML- and ADR-resistant cells, and silencing ALKBH5 reduced the ADR resistance of AML cells. ALKBH5 removed m6A modifications from TUG1, disrupting the interaction between YTHDF2 and TUG1, thereby stabilizing TUG1 expression. TUG1 bound to EHMT2, promoting H3K9me2 modification on the SH3BGRL promoter and suppressing SH3BGRL expression. Overexpression of TUG1 or knockdown of SH3BGRL reversed the suppressive effect of ALKBH5 knockdown on ADR resistance. In vivo, ALKBH5 knockdown inhibited ADR resistance in AML cells. In conclusion, ALKBH5 removed m6A modification to stabilize TUG1 expression in a YTHDF2-dependent manner, enhancing H3K9me2 levels on the SH3BGRL promoter and suppressing SH3BGRL expression, thus promoting ADR resistance in AML cells.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hsa_circ_0001492 regulates the hsa-miR-145-5p/ovarian carcinoma immunoreactive antigen domain 2 axis to promote the progression of lung adenocarcinoma. Hsa_circ_0001492调节hsa-miR-145-5p/卵巢癌免疫反应抗原域2轴，促进肺腺癌的进展。

Biomolecules & biomedicine Pub Date : 2024-10-26 DOI: 10.17305/bb.2024.11140

Yuanqiang He, Gang Li, Ran Fu, Yue Li, Ying Wang

{"title":"Hsa_circ_0001492 regulates the hsa-miR-145-5p/ovarian carcinoma immunoreactive antigen domain 2 axis to promote the progression of lung adenocarcinoma.","authors":"Yuanqiang He, Gang Li, Ran Fu, Yue Li, Ying Wang","doi":"10.17305/bb.2024.11140","DOIUrl":"https://doi.org/10.17305/bb.2024.11140","url":null,"abstract":"Circular RNA (circRNA) has been proven to be a key regulator in a range of tumor illnesses, such as lung adenocarcinoma (LUAD); however, the regulatory mechanisms of circRNA remain unclear. In this study, circRNA (hsa_circ_0001492) in LUAD was examined for its regulatory and functional potential. qRT-PCR was used to assess the hsa_circ_0001492 level in LUAD. The RNAse R digestion test was employed to isolate hsa_circ_0001492. The primary location of hsa_circ_0001492 enrichment in LUAD cells was identified through a nucleoplasmic separation test. LUAD cell migration, proliferation, and spherogenicity were examined using wound healing, transwell, EdU, and cell spherogenicity assays. The association between miR-145-5p and hsa_circ_0001492/ovarian carcinoma immunoreactive antigen domain 2 (OCIAD2) was validated using a dual luciferase experiment. The interaction between sh-hsa_circ_0001492 and miR-145-5p was confirmed through an RNA pull-down assay. The effects of hsa_circ_0001492, miR-145-5p, and OCIAD2 on LUAD tumor development were examined using xenograft mouse models and immunohistochemistry tests. Results showed a higher amount of hsa_circ_0001492 in LUAD. The cytoplasm of LUAD cells was observed in the area where hsa_circ_0001492 mainly accumulated; hsa_circ_0001492 enhanced LUAD cell migration, proliferation, and sphere-forming ability. MiR-145-5p and OCIAD2 were identified as targets of hsa_circ_0001492 and miR-145-5p, respectively. The level of OCIAD2 was increased by hsa_circ_0001492 through targeted binding to miR-145-5p. In nude mice, tumor growth was inhibited by silencing hsa_circ_0001492, while knockdown of miR-145-5p and overexpression of OCIAD2 promoted the growth of LUAD tumors. In conclusion, hsa_circ_0001492 regulates the hsa-miR-145-5p/OCIAD2 axis to promote the progression of LUAD, and could be a useful target for the diagnosis and treatment of LUAD.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The gut microbiota modulates airway inflammation in allergic asthma through the gut-lung axis related immune modulation: A review. 肠道微生物群通过与免疫调节相关的肠肺轴调节过敏性哮喘的气道炎症：综述。

Biomolecules & biomedicine Pub Date : 2024-10-26 DOI: 10.17305/bb.2024.11280

Meng Zhang, Ziwen Qin, Chuanjun Huang, Bin Liang, Xiuqing Zhang, Weitao Sun

{"title":"The gut microbiota modulates airway inflammation in allergic asthma through the gut-lung axis related immune modulation: A review.","authors":"Meng Zhang, Ziwen Qin, Chuanjun Huang, Bin Liang, Xiuqing Zhang, Weitao Sun","doi":"10.17305/bb.2024.11280","DOIUrl":"10.17305/bb.2024.11280","url":null,"abstract":"The human gut microbiota is a vast and complex microbial community. According to statistics, the number of bacteria residing in the human intestinal tract is approximately ten times that of total human cells, with over 1000 different species. The interaction between the gut microbiota and various organ tissues plays a crucial role in the pathogenesis of local and systemic diseases, exerting a significant influence on disease progression. The relationship between the gut microbiota and intestinal diseases, along with its connection to the pulmonary immune environment and the development of lung diseases, is commonly referred to as the “gut–lung axis.” The incidence of bronchial asthma is rising globally. With ongoing research on gut microbiota, it is widely believed that intestinal microorganisms and their metabolic products directly or indirectly participate in the occurrence and development of asthma. Based on the gut–lung axis, this review examines recent research suggesting that the intestinal microbiota can influence the occurrence and progression of allergic asthma through the modulation of cytokine immune balance and mucosal integrity. Though the precise immune pathways or microbial species influencing asthma through the gut–lung axis are still under exploration, summarizing the immune modulation through the gut–lung axis in allergic asthma may provide insights for the clinical management of the condition.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene polymorphisms of miR-323b and miR-1343 that regulate kininogen L are associated with schizophrenia susceptibility: A preliminary population‑based study. 调控激肽原 L 的 miR-323b 和 miR-1343 基因多态性与精神分裂症易感性有关：一项基于人群的初步研究。

Biomolecules & biomedicine Pub Date : 2024-10-25 DOI: 10.17305/bb.2024.11100

Xiaoyu Liu, Mengdi Jin, Mingjia Yang, Lijuan Yan, Weijiao Zhao, Lizhuo Liu, Hongmin Wang, Yongzhuo Ding, Yanyan Sun, Yanchi Zhang, Qiong Yu

{"title":"Gene polymorphisms of miR-323b and miR-1343 that regulate kininogen L are associated with schizophrenia susceptibility: A preliminary population‑based study.","authors":"Xiaoyu Liu, Mengdi Jin, Mingjia Yang, Lijuan Yan, Weijiao Zhao, Lizhuo Liu, Hongmin Wang, Yongzhuo Ding, Yanyan Sun, Yanchi Zhang, Qiong Yu","doi":"10.17305/bb.2024.11100","DOIUrl":"https://doi.org/10.17305/bb.2024.11100","url":null,"abstract":"miR-SNP is a type of functional single nucleotide polymorphism (SNP) that affects the regulatory functions of miRNA genes, miRNA binding sites, or components of miRNA biogenesis. This study aimed to explore the relationship between miRNA gene polymorphisms that regulate the kininogen L protein and schizophrenia (SCZ). Bioinformatics methods predicted miRNA gene polymorphism sites regulating the kininogen L protein. The polymorphisms of rs56103835, rs6513496, rs651349, and rs2986407 were detected using imLDR multiple SNP typing technologies in 513 SCZ patients and 509 controls. The association of miR-SNP variations with SCZ susceptibility and symptoms was evaluated using SNPstat to determine the optimal inheritance model. Generalized multifactor dimensionality reduction (GMDR) analysis and logistic regression were used to calculate miR-SNP interactions. The association between hsa-miR-323b-rs56103835 and SCZ was statistically significant under the dominant model. The result of gene-gene interaction showed that the three-factor model (rs56103835 / rs2986407 / rs2155248) was the best, but it could not be considered significantly related to SCZ. Additionally, SCZ patients with the CC or CT genotype on rs2986407 were more likely to experience auditory hallucinations than those with the TT genotype. Our data revealed that the mutation of hsa-miR-323b-rs56103835 from C to T was associated with susceptibility to SCZ. The mutation of hsa-miR-1343-rs2986407 from T to C increases the risk of auditory hallucinations in SCZ patients.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the proteomic signatures of coronary artery disease and hypercholesterolemia. 揭示冠心病和高胆固醇血症的蛋白质组特征。

Biomolecules & biomedicine Pub Date : 2024-10-24 DOI: 10.17305/bb.2024.10111

Gulsen Guliz Anlar, Shona Pedersen, Sarah Al Ashmar, Hubert Krzyslak, Layla Kamareddine, Asad Zeidan

{"title":"Unraveling the proteomic signatures of coronary artery disease and hypercholesterolemia.","authors":"Gulsen Guliz Anlar, Shona Pedersen, Sarah Al Ashmar, Hubert Krzyslak, Layla Kamareddine, Asad Zeidan","doi":"10.17305/bb.2024.10111","DOIUrl":"10.17305/bb.2024.10111","url":null,"abstract":"Atherosclerosis, a leading cause of coronary artery disease (CAD), is heavily influenced by hypercholesterolemia (HC). Proteomics research has shown promise in identifying biological markers for CAD diagnosis and prognosis. This cross-sectional study aimed to identify novel biomarkers for detecting HC and CAD. Through the analysis of proteome data from healthy controls (n = 45) and patients diagnosed with HC (n = 51) or CAD (n = 32), distinct protein patterns associated with each condition were identified. Significant alterations in protein levels were identified with a false discovery rate (FDR)-corrected q-value of <0.05. Subsequent receiver operating characteristic (ROC) analysis, with an area under the curve (AUC) greater than 0.75, was conducted. CAD patients exhibited significantly increased levels of the cholesterol-metabolizing protein proprotein convertase subtilisin/kexin type 9 (PCSK9) and varied levels of the angiogenesis-related protein stromal-cell-derived factor-1 (SDF-1) compared to controls. In pairwise comparisons among the study groups, 65 proteins showed significant differential expression. Notably, 14 of these proteins had significant correlations with blood cholesterol levels. Additionally, 22 of the identified proteins were associated with CAD or HC pathways, with nine proteins being common to both conditions (APO E, APO E3, MMP-3, PCSK9, SDF-1, APO B, PAFAH, 60 kDa heat shock protein, and TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 fusion). Nevertheless, this is an exploratory study, and validation studies are needed to confirm these potential protein biomarkers for CAD in the context of HC.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The diagnostic accuracy of exosomes for glioma: A meta-analysis. 外泌体对胶质瘤的诊断准确性：荟萃分析

Biomolecules & biomedicine Pub Date : 2024-10-22 DOI: 10.17305/bb.2024.11268

XiangMin Zhang, YanDi Tan, XiaoYa He, Jie Huang, XiaoYing Ni, Qian Hu, JinHua Cai

{"title":"The diagnostic accuracy of exosomes for glioma: A meta-analysis.","authors":"XiangMin Zhang, YanDi Tan, XiaoYa He, Jie Huang, XiaoYing Ni, Qian Hu, JinHua Cai","doi":"10.17305/bb.2024.11268","DOIUrl":"https://doi.org/10.17305/bb.2024.11268","url":null,"abstract":"Glioma is one of the most prevalent primary intracranial tumors, and biomarker testing offers a non-invasive modality with high diagnostic efficiency. The aim of this meta-analysis is to evaluate the diagnostic effectiveness of exosomes as biomarkers for glioma. We included 16 studies on exosomes as biomarkers for gliomas. The pooled sensitivity (SEN), specificity (SPE), positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) for 25 biomarkers across these 16 studies were as follows: 82% (95% CI: 0.77-0.86), 91% (95% CI: 0.86-0.94), 9.10 (95% CI: 5.64-14.68), 0.20 (95% CI: 0.16-0.25), 45.94 (95% CI: 25.40-83.09), and 0.92 (95% CI: 0.89-0.94), respectively. Meta-regression indicated that biomarker analysis, biomarker type, and sample size may be sources of heterogeneity. Subgroup analysis suggested that ultracentrifugation (UC) was a better method for extracting exosomes. miRNA and other RNA groups (sncRNA, lncRNA, circRNA) provided higher SEN (0.88 vs. 0.84 vs. 0.78) compared to proteins. This study demonstrates the superior diagnostic efficacy of exosomes as biomarkers for gliomas, with high accuracy in diagnosing gliomas.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin regulates sensitivity to X-ray radiation of hepatocellular carcinoma through miR-216a-3p. 槲皮素通过 miR-216a-3p 调节肝细胞癌对 X 射线辐射的敏感性

Biomolecules & biomedicine Pub Date : 2024-10-22 DOI: 10.17305/bb.2024.11125

Nuran Bedolla, Linyu Liu, Qiuxian Xie, Xueting Liu, Yanli Ren

{"title":"Quercetin regulates sensitivity to X-ray radiation of hepatocellular carcinoma through miR-216a-3p.","authors":"Nuran Bedolla, Linyu Liu, Qiuxian Xie, Xueting Liu, Yanli Ren","doi":"10.17305/bb.2024.11125","DOIUrl":"https://doi.org/10.17305/bb.2024.11125","url":null,"abstract":"Hepatocellular carcinoma (HCC) is a highly aggressive liver cancer with limited therapeutic options, and enhancing radiosensitivity remains a key challenge in improving treatment outcomes. Quercetin (Que) can inhibit the progression of hepatocellular carcinoma (HCC); however, its effect on HCC radiosensitivity remains unclear. This research investigates the role of Que in regulating HCC growth and radiosensitivity, aiming to provide a scientific foundation for enhancing the clinical efficacy of radiation therapy in HCC. The CCK-8 assay was used to determine the optimal treatment conditions for Que and X-rays. Changes in cell growth, cycle arrest, invasion, migration, the relative proportion of JC-1 red and green fluorescence (mitochondrial membrane potential), and the levels of ROS, MDA, SOD, and GSH-Px (oxidative stress) were assessed using flow cytometry, Transwell assays, JC-1 staining, Western blot, and ELISA, respectively, under Que, X-ray, and co-treatment conditions. The effect of miR-216a-3p knockdown on the action of Que was also explored, and the potential pathways by which Que regulates HCC growth and radiosensitivity were investigated in conjunction with in vivo subcutaneous transplantation tumor experiments. The in vitro treatment parameters for Que and X-rays were 100 µM and 4 Gy. Que combined with X-ray therapy enhanced HCC cell radiosensitivity, reduced proliferation, invasion, and migration, and promoted oxidative stress and apoptosis. Que was found to upregulate miR-216a-3p in HCC cells. Rescue experiments with miR-216a-3p knockdowns demonstrated that Que regulates HCC cell radiosensitivity via miR-216a-3p. In vivo research further showed that Que increased tumor sensitivity to X-rays by upregulating miR-216a-3p, thereby inhibiting HCC growth. In conclusion, Que has been shown to enhance HCC radiosensitization by upregulating miR-216a-3p and inhibiting HCC progression. Que may be a promising agent for increasing the radiosensitivity of HCC.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surgical Approach and Recurrence Risk in Struma Ovarii: A Retrospective and Systematic Analysis. 手术方法与卵巢瘤复发风险：一项回顾性系统分析。

Biomolecules & biomedicine Pub Date : 2024-10-20 DOI: 10.17305/bb.2024.11287

Miao Ao, You Wu, Zhiping Huo, He Zhang, Wei Mao, Bin Li

{"title":"Surgical Approach and Recurrence Risk in Struma Ovarii: A Retrospective and Systematic Analysis.","authors":"Miao Ao, You Wu, Zhiping Huo, He Zhang, Wei Mao, Bin Li","doi":"10.17305/bb.2024.11287","DOIUrl":"10.17305/bb.2024.11287","url":null,"abstract":"Struma ovarii (SO) represents a rare subset of ovarian germ cell tumors, with approximately 5% transforming into malignant SO (MSO). This study retrospectively analyzed clinical data from 23 SO patients treated at the Cancer Hospital of the Chinese Academy of Medical Sciences between January 2013 and December 2023, including 17 benign SO and 6 MSO cases. Additionally, a systematic review of 164 cases of MSO confined to the ovary, reported in the literature from 1946 to 2024, was conducted. Data on pathological type, treatment, and prognosis were extracted, and univariate and multivariate Cox regression analyses were performed to identify risk factors for recurrence in stage I MSO. The median age at diagnosis was higher for benign SO compared to MSO (58 vs. 42.5 years), with 70.6% of patients being postmenopausal. Benign SO commonly presented with abdominal distension or mass, with more than half having ascites, while MSO patients were asymptomatic and lacked ascites. Cox regression analyses revealed that ovarian cystectomy was adversely associated with recurrence risk in stage I MSO, likely due to surgically induced capsular rent and potential tumor spillage. Significantly lower recurrence risks were observed in patients who underwent unilateral or bilateral salpingo-oophorectomy (HR = 0.36, P = 0.019; HR = 0.19, P = 0.004, respectively). This study highlights the importance of the surgical approach in the management of stage I MSO. A thorough preoperative discussion of the benefits and risks of different surgical approaches is recommended for patients desiring fertility preservation. Postoperative adjuvant therapy has not been shown to have a significant impact on prognosis. For the treatment of recurrent MSO, selecting appropriate surgical and adjuvant therapeutic strategies is essential to improve the long-term prognosis of MSO patients.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0