Biomolecules & biomedicine最新文献

Inflammatory biomarkers and plasma lipidomics after raltegravir plus TDF/FTC initiation in people with HIV: A longitudinal pilot study. 艾滋病毒感染者在雷替重力韦加TDF/FTC治疗后的炎症生物标志物和血浆脂质组学：一项纵向试点研究

Biomolecules & biomedicine Pub Date : 2026-10-19 DOI: 10.17305/bb.2026.14928

Jose-Ramon Blanco, Enrique Bernal-Morell, Santiago Moreno, David Rial-Crestelo, María Martínez-Vives, Cristina Alonso, Leire Pérez-Latorre, Sergio Reus Bañuls, Lourdes Romero, Xabier Camino Ortiz de Barrón, Laura Perez-Martinez

{"title":"Inflammatory biomarkers and plasma lipidomics after raltegravir plus TDF/FTC initiation in people with HIV: A longitudinal pilot study.","authors":"Jose-Ramon Blanco, Enrique Bernal-Morell, Santiago Moreno, David Rial-Crestelo, María Martínez-Vives, Cristina Alonso, Leire Pérez-Latorre, Sergio Reus Bañuls, Lourdes Romero, Xabier Camino Ortiz de Barrón, Laura Perez-Martinez","doi":"10.17305/bb.2026.14928","DOIUrl":"https://doi.org/10.17305/bb.2026.14928","url":null,"abstract":"Persistent inflammation and altered lipid metabolism are linked to comorbidities in people with human immunodeficiency virus (HIV), yet their progression after initiating antiretroviral therapy (ART) is not fully understood. This exploratory, uncontrolled pilot study investigated longitudinal changes in biomarkers and plasma lipidomics in 11 ART-naïve adults starting raltegravir plus tenofovir disoproxil fumarate/emtricitabine. Circulating biomarkers were measured, and untargeted plasma lipidomics was performed using ultra-high-performance liquid chromatography-mass spectrometry at baseline and at a follow-up visit near week 48 (range: 47.7-61.9 weeks). Within-participant changes were analyzed using paired Wilcoxon signed-rank tests, with Benjamini-Hochberg false discovery rate (FDR) correction applied separately to biomarker and lipidomic datasets. Paired effect sizes and 95% confidence intervals were also estimated. All participants achieved virological suppression. After FDR correction, interleukin-10 (IL-10) concentrations increased, and soluble cluster of differentiation 163 (sCD163) concentrations decreased (both q = 0.019). However, none of the 490 lipid features remained significant after FDR correction. Twenty-six features showed large absolute paired Hedges' effect-size estimates (|gz| ≥ 0.80), with 24 positive and two negative estimates. These findings demonstrate longitudinal changes in IL-10 and sCD163 following ART initiation. The lipidomic results, however, are considered hypothesis-generating. Due to the absence of a comparator group, these changes cannot be definitively attributed to the specific ART regimen. Larger, controlled studies are necessary to confirm reproducibility and clinical relevance.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PMEPA1 in laryngeal squamous cell carcinoma: Prognostic value, immune associations, and predicted drug sensitivity. PMEPA1在喉部鳞状细胞癌中的预后价值、免疫关联和预测药物敏感性

Biomolecules & biomedicine Pub Date : 2026-10-19 DOI: 10.17305/bb.2026.14433

Ping Zhang, Haiying Wu, Jinfeng He, Dong Zhang, Jiliang Yi

{"title":"PMEPA1 in laryngeal squamous cell carcinoma: Prognostic value, immune associations, and predicted drug sensitivity.","authors":"Ping Zhang, Haiying Wu, Jinfeng He, Dong Zhang, Jiliang Yi","doi":"10.17305/bb.2026.14433","DOIUrl":"https://doi.org/10.17305/bb.2026.14433","url":null,"abstract":"Reliable prognostic biomarkers for laryngeal squamous cell carcinoma (LSCC) remain limited. This study evaluated prostate transmembrane protein androgen induced 1 (PMEPA1) expression, prognostic significance, functional effects, and immune and drug sensitivity associations. Transcriptomic and clinical data from The Cancer Genome Atlas and Gene Expression Omnibus were analyzed using Kaplan-Meier analysis, Cox regression, functional enrichment, immune deconvolution, and drug sensitivity prediction. Expression and knockdown effects were assessed using quantitative reverse transcription polymerase chain reaction, western blotting, cell viability assays, and Transwell assays. PMEPA1 was upregulated in tumors and cancer cell lines. Higher expression was associated with poorer overall survival in two cohorts and remained significant after multivariable adjustment in the discovery cohort. Areas under the receiver operating characteristic curve (AUCs) for tumor-normal discrimination ranged from 0.835 to 0.944; nomogram AUCs were 0.766, 0.772, and 0.809 at 1, 3, and 5 years. Enrichment analyses highlighted extracellular matrix organization. PMEPA1 knockdown reduced viability, migration, and invasion in one LSCC cell line. Computational analyses linked higher expression to greater proportions of unpolarized (M0) and alternatively activated (M2) macrophages, lower cluster of differentiation 8-positive (CD8+) T-cell proportions, and higher stromal and immune exclusion scores. Expression correlated positively with predicted half-maximal inhibitory concentrations (IC₅₀) of entinostat and vorinostat and negatively with those of dasatinib and AZD1332. PMEPA1 is a candidate prognostic biomarker with functional relevance in LSCC; its immune and drug sensitivity associations require experimental and clinical validation.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PAI-1-IL-17 statistical interaction and periventricular white matter hyperintensity severity in sporadic cerebral small vessel disease. 散发性脑血管病中PAI-1-IL-17的统计相互作用与脑室周围白质高强度的严重程度。

Biomolecules & biomedicine Pub Date : 2026-10-16 DOI: 10.17305/bb.2026.14993

Yang Zilong, Hou Chunlan, Wang Baoai

{"title":"PAI-1-IL-17 statistical interaction and periventricular white matter hyperintensity severity in sporadic cerebral small vessel disease.","authors":"Yang Zilong, Hou Chunlan, Wang Baoai","doi":"10.17305/bb.2026.14993","DOIUrl":"https://doi.org/10.17305/bb.2026.14993","url":null,"abstract":"The factors influencing the severity of periventricular white matter hyperintensity (WMH) in cerebral small vessel disease (CSVD) are not fully understood. This study investigated the multiplicative interaction between circulating plasminogen activator inhibitor-1 (PAI-1) and interleukin-17 (IL-17) concerning periventricular WMH severity. In this single-center, cross-sectional study, we enrolled 151 patients with sporadic CSVD, selected from 526 consecutively screened inpatients. Serum levels of PAI-1, IL-17, and matrix metalloproteinase-9 (MMP-9) were quantified using enzyme-linked immunosorbent assays. Regional WMH was graded on magnetic resonance imaging using the Fazekas scale. Multivariable logistic regression was employed to assess the PAI-1-IL-17 interaction for moderate-to-severe periventricular WMH (Fazekas scores 2-3 vs. 0-1), with additional adjustments for MMP-9 and sensitivity analyses. The primary interaction odds ratio (OR) was 1.72 per one-standard-deviation increase in the standardized product term (95% confidence interval [CI]: 1.07-2.77; p = 0.026). Adjusting for MMP-9 did not substantially change this estimate. The interaction was not statistically significant in the ordinal periventricular analysis (OR: 1.40; 95% CI: 0.99-1.99; p = 0.061) or the deep-WMH model (OR: 0.83; 95% CI: 0.51-1.37; p = 0.468). While alternative binary models showed positive interaction estimates, resampling analyses indicated limited stability, and additive interaction lacked consistent support. These exploratory findings suggest a multiplicative statistical interaction between PAI-1 and IL-17 for dichotomized periventricular WMH severity. However, they do not establish causality, biological synergy, or clinical biomarker utility. Prospective multicenter validation is essential.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early-treatment coagulation activation and radiographic progression during VEGF/VEGFR inhibitor therapy: A multicancer real-world cohort study. 在VEGF/VEGFR抑制剂治疗期间的早期治疗凝血激活和影像学进展：一项多癌症真实世界队列研究。

Biomolecules & biomedicine Pub Date : 2026-10-15 DOI: 10.17305/bb.2026.15049

Yingda Liu, Bai Liu, Su Liu, Teng Zhang, Tianye Lv, Sibei Han, Huichao Li, Shuo Sun, Xin Zhou

{"title":"Early-treatment coagulation activation and radiographic progression during VEGF/VEGFR inhibitor therapy: A multicancer real-world cohort study.","authors":"Yingda Liu, Bai Liu, Su Liu, Teng Zhang, Tianye Lv, Sibei Han, Huichao Li, Shuo Sun, Xin Zhou","doi":"10.17305/bb.2026.15049","DOIUrl":"https://doi.org/10.17305/bb.2026.15049","url":null,"abstract":"Coagulation activation is common in cancer, but its link to treatment durability is unclear. We investigated whether early-treatment coagulation activation correlated with radiographic progression during vascular endothelial growth factor (VEGF)/vascular endothelial growth factor receptor (VEGFR) inhibitor therapy. This single-center, retrospective, multicancer cohort study developed a coagulation activation score (0-2) based on the last available D-dimer (>500 ng/mL) and fibrinogen (>4.0 g/L) measurements taken within 100 days after baseline. Multivariable Cox models calculated hazard ratios (HRs) and 95% confidence intervals (CIs) for subsequent radiographic time to progression among patients who were progression-free at the 100-day landmark. Of 1400 eligible patients, 1089 had evaluable early-treatment measurements, and 909 were included in the landmark analysis. Compared to a score of 0, adjusted HRs were 1.29 (95% CI 1.02-1.62) for score 1 and 1.51 (95% CI 1.07-2.12) for score 2. Score 2 also correlated with lower odds of durable clinical benefit (adjusted odds ratio 0.56, 95% CI 0.38-0.84), while objective response and best tumor shrinkage did not show a consistent gradient. These associations remained significant in time-dependent and timing-restricted analyses. Exploratory subgroup analyses revealed the strongest association in colorectal cancer patients treated with bevacizumab (score 2 vs. 0: HR 2.23, 95% CI 1.21-4.11); however, no association was observed in lung cancer patients treated with bevacizumab. No significant interaction with documented immune checkpoint inhibitor exposure was detected. The score did not enhance discrimination beyond clinical variables and baseline coagulation markers. Early-treatment coagulation activation was associated with reduced treatment durability during VEGF/VEGFR inhibition. Prospective validation in specific cancer-drug contexts is necessary.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Independent association of the LNG index with extrahepatic metastasis in hepatocellular carcinoma. LNG指数与肝细胞癌肝外转移的独立关联。

Biomolecules & biomedicine Pub Date : 2026-10-15 DOI: 10.17305/bb.2026.14893

Huijiao Wang, Zixuan Huang, Xingchi Dong, Dehua Wang, Rongzhen Dong, Jialu Wang, Miao Wang, Jixian Meng, Huanwei Zheng, Huimin Yan

{"title":"Independent association of the LNG index with extrahepatic metastasis in hepatocellular carcinoma.","authors":"Huijiao Wang, Zixuan Huang, Xingchi Dong, Dehua Wang, Rongzhen Dong, Jialu Wang, Miao Wang, Jixian Meng, Huanwei Zheng, Huimin Yan","doi":"10.17305/bb.2026.14893","DOIUrl":"https://doi.org/10.17305/bb.2026.14893","url":null,"abstract":"Extrahepatic metastasis (EHM) indicates a poor prognosis in hepatocellular carcinoma (HCC). This study evaluated the association of the logarithmically transformed neutrophil-to-lymphocyte ratio-gamma-glutamyl transferase index (LNG) with EHM at initial assessment and its incremental discriminative value. This single-center retrospective cross-sectional study included 678 adults with newly diagnosed HCC. LNG was calculated as the natural logarithm of the product of its components. Associations were assessed using multivariable logistic and linear regression and restricted cubic splines. Model performance was evaluated using receiver operating characteristic, reclassification, and decision curve analyses. EHM was present in 270 patients (39.8%). The adjusted odds ratio (OR) per unit increase in LNG was 1.25 (95% confidence interval [CI]: 1.06-1.48; p = 0.007). Adjusted spline analysis indicated nonlinearity (p = 0.009). Compared with the lowest tertile, adjusted ORs were 2.45 (95% CI: 1.45-4.17) and 2.59 (95% CI: 1.44-4.69) in the middle and highest tertiles, respectively. LNG yielded an area under the receiver operating characteristic curve (AUC) of 0.748 (95% CI: 0.711-0.785). In an exploratory analysis, the AUC was 0.717 (95% CI: 0.652-0.783) among patients with alpha-fetoprotein (AFP) levels <20 ng/mL. Adding LNG to a baseline model, which included age, sex, tumor size, AFP, multifocality, and cirrhosis, increased the AUC from 0.762 to 0.797 (p < 0.001). This also improved reclassification and led to a greater estimated net benefit. Among patients with EHM, LNG was positively associated with albumin-bilirubin and Controlling Nutritional Status scores and platelet-to-lymphocyte ratio. LNG may complement initial EHM assessment in HCC, though external validation is required.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149208312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moderate carpal tunnel syndrome: Factors associated with long-term surgery risk after corticosteroid injection. 中度腕管综合征：皮质类固醇注射后长期手术风险的相关因素。

Biomolecules & biomedicine Pub Date : 2026-10-13 DOI: 10.17305/bb.2026.15005

Meral Bilgilisoy Filiz, Nur Altın, Muhammet Hüseyin Sarı, Hanife Hale Hekim, Ülkü Uçar, Tuncay Çakır, Şebnem Koldaş Doğan

{"title":"Moderate carpal tunnel syndrome: Factors associated with long-term surgery risk after corticosteroid injection.","authors":"Meral Bilgilisoy Filiz, Nur Altın, Muhammet Hüseyin Sarı, Hanife Hale Hekim, Ülkü Uçar, Tuncay Çakır, Şebnem Koldaş Doğan","doi":"10.17305/bb.2026.15005","DOIUrl":"https://doi.org/10.17305/bb.2026.15005","url":null,"abstract":"Corticosteroid injection is commonly used for moderate carpal tunnel syndrome (CTS), but factors associated with subsequent surgery remain unclear. This study evaluated baseline clinical, electrophysiological, and ultrasonographic factors associated with long-term surgery risk. This retrospective cohort included 73 of 84 participants with electrodiagnostically moderate CTS from a randomized trial comparing ultrasound-guided and landmark-guided injections. All received a single corticosteroid injection at baseline. Carpal tunnel release was ascertained through structured telephone interviews, with dates verified in national records for 16 of 17 events. Time to surgery was analyzed using Kaplan-Meier estimates and separate two-predictor Cox models. During a median follow-up of 54.7 months, 17 patients (23.3%) underwent surgery. Estimated 48-month surgery-free survival was 79.5%, with a 95% confidence interval (CI) of 68.3-87.1%. Higher baseline visual analog scale pain scores during activity and summed Boston Carpal Tunnel Questionnaire symptom severity scores were associated with surgery in separate adjusted models: hazard ratio (HR) per point 1.51 (95% CI, 1.14-2.00) and HR 1.11 (95% CI, 1.04-1.17), respectively. Longer motor distal latency (MDL) was associated with surgery in both models (HR per millisecond 1.90, 95% CI, 1.05-3.43; and 2.30, 95% CI, 1.24-4.29, respectively). Statistical support weakened with ridge penalization, particularly for MDL. No associations were detected for ultrasonographic measures or injection technique. Greater baseline symptom burden and prolonged MDL were associated with subsequent surgery, but these exploratory findings require validation in larger prospective cohorts before informing treatment selection.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of plant extract formulations on anxiety-like behavior, sociability, and gut microbiota diversity in paracetamol-treated adult zebrafish. 植物提取物配方对扑热息痛处理的成年斑马鱼的焦虑样行为、社交能力和肠道微生物群多样性的影响。

Biomolecules & biomedicine Pub Date : 2026-10-12 DOI: 10.17305/bb.2026.14533

Oana-Georgiana Oprea, Ioana-Miruna Balmus, Mitica Ciorpac, Tudor Ilie, Gabriel Plavan, Mircea Nicusor Nicoara, Lucian Gorgan, Bogdan Stoica, Bogdan-Ionel Tamba, Ramona-Alexandra Ciausu, Alin Ciobica, Anca Trifan

{"title":"Effects of plant extract formulations on anxiety-like behavior, sociability, and gut microbiota diversity in paracetamol-treated adult zebrafish.","authors":"Oana-Georgiana Oprea, Ioana-Miruna Balmus, Mitica Ciorpac, Tudor Ilie, Gabriel Plavan, Mircea Nicusor Nicoara, Lucian Gorgan, Bogdan Stoica, Bogdan-Ionel Tamba, Ramona-Alexandra Ciausu, Alin Ciobica, Anca Trifan","doi":"10.17305/bb.2026.14533","DOIUrl":"https://doi.org/10.17305/bb.2026.14533","url":null,"abstract":"Plant extract-based formulations may influence behavior and gut microbiota, but their effects following acute paracetamol (PAR) exposure remain unclear. This preliminary study evaluated the behavioral effects of two formulations, coded MOD and TEL, and explored TEL-associated microbiota changes in adult zebrafish. Sixty fish were randomized to six groups (10/group). PAR exposure (5 mM for 24 h) was followed by formulation treatment (500 mg/L for five days), alongside untreated, PAR-only, and formulation-only controls. Behavior was assessed using the novel tank test (NTT) and social behavior test. Full-length 16S ribosomal ribonucleic acid (rRNA) gene sequencing was performed in untreated, PAR-only, and PAR-plus-TEL groups (three fish/group). Compared with PAR alone, MOD increased swimming distance and velocity during social testing (p = 0.028 and p = 0.003, respectively), whereas TEL reduced vertical crossings in the NTT (p = 0.035) and time spent near social stimuli (p = 0.0033). Reductions in freezing duration with both formulations were not statistically significant compared with PAR alone. No significant differences in alpha or beta diversity were detected, and no genus-level differences remained significant after multiple-testing correction. These exploratory findings suggest formulation- and test-dependent behavioral responses without statistically supported microbiota changes. Larger studies with replicated tanks and deeper sequencing are needed to clarify these associations.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149170593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tear interleukin-6 and clinical activity in thyroid eye disease. 泪液白细胞介素-6与甲状腺眼病的临床活性。

Biomolecules & biomedicine Pub Date : 2026-10-08 DOI: 10.17305/bb.2026.14860

Nayeli Alcaraz-Lares, Daniel B Mejia-Llanes, Angel Nava-Castañeda

{"title":"Tear interleukin-6 and clinical activity in thyroid eye disease.","authors":"Nayeli Alcaraz-Lares, Daniel B Mejia-Llanes, Angel Nava-Castañeda","doi":"10.17305/bb.2026.14860","DOIUrl":"https://doi.org/10.17305/bb.2026.14860","url":null,"abstract":"Thyroid eye disease (TED) is an autoimmune orbital disorder in which interleukin-6 (IL-6) may reflect inflammatory activity. We evaluated tear IL-6 concentrations across disease activity groups, their association with the Clinical Activity Score (CAS), and paired tear-serum differences. This prospective cross-sectional study included 42 adults: 15 with active TED (CAS ≥3), 14 with inactive TED (CAS 0-2), and 13 healthy controls. IL-6 was measured in diluted tear samples collected by micro-volume wash and paired serum using cytometric bead array with flow cytometry. Analyses comprised one-way analysis of variance, paired Student t-tests, and Pearson correlation. Mean tear IL-6 concentrations were 22.33, 8.49, and 3.82 pg/mL in active TED, inactive TED, and controls, respectively, with a significant overall difference across groups (p = 0.002). Tear IL-6 concentrations exceeded serum concentrations in active and inactive TED (p = 0.001 and p = 0.005, respectively), with no significant difference in controls (p = 0.309). In active TED, tear IL-6 correlated positively with CAS (r = 0.55; 95% confidence interval, 0.05-0.83; p = 0.032). These exploratory findings support further investigation of tear IL-6 as a correlate of clinical activity. Unmeasured ocular-surface status, unquantified tear dilution, and extrapolation below the assay's lowest calibrator limit interpretation; larger longitudinal studies incorporating objective ocular-surface assessment and assays validated for tear fluid are needed before clinical application.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum FTIR spectral features associated with HDRA-defined gemcitabine sensitivity in cholangiocarcinoma. 血清FTIR光谱特征与hdra定义的胆管癌患者吉西他滨敏感性相关。

Biomolecules & biomedicine Pub Date : 2026-10-07 DOI: 10.17305/bb.2026.14765

Kanokporn Matra, Patutong Chatchawal, Molin Wongwattanakul, Patipat Rachawangmuang, Sutthiwan Janthamala, Thanaphong Rattanathip, Sirinart Chio-Srichan, Chanchai Hongsa, Malinee Thanee, Supakan Amontailak, Juthamas Jaimanee, Piyapong Simtong, Anchalee Techasen

{"title":"Serum FTIR spectral features associated with HDRA-defined gemcitabine sensitivity in cholangiocarcinoma.","authors":"Kanokporn Matra, Patutong Chatchawal, Molin Wongwattanakul, Patipat Rachawangmuang, Sutthiwan Janthamala, Thanaphong Rattanathip, Sirinart Chio-Srichan, Chanchai Hongsa, Malinee Thanee, Supakan Amontailak, Juthamas Jaimanee, Piyapong Simtong, Anchalee Techasen","doi":"10.17305/bb.2026.14765","DOIUrl":"https://doi.org/10.17305/bb.2026.14765","url":null,"abstract":"Cholangiocarcinoma (CCA) exhibits diverse responses to chemotherapy, underscoring the need for drug sensitivity biomarkers. This proof-of-concept study utilized Fourier transform infrared (FTIR) spectral features to identify ex vivo gemcitabine sensitivity and resistance, as determined by the histoculture drug response assay (HDRA). We analyzed ten formalin-fixed paraffin-embedded tumor samples and 21 serum samples (including seven matched pairs) using synchrotron radiation-based FTIR microspectroscopy and attenuated total reflectance FTIR spectroscopy, respectively. Tissue and serum spectra were evaluated using partial least squares discriminant analysis and principal component analysis, respectively, alongside patient-level spectral ratio comparisons. Both sample types primarily displayed differences in the protein-associated amide I and II regions, with additional variations in carbohydrate- and nucleic acid-associated regions. Tissue spectra demonstrated partial group separation, while serum spectra showed exploratory separation with some overlap. Three serum intensity ratios (1633/1649 cm⁻¹, 1539/1649 cm⁻¹, and 1539/1633 cm⁻¹) remained significantly different after Benjamini-Hochberg correction (adjusted p = 0.041 for each), whereas no significant differences were observed in tissue ratios. Serum-based FTIR spectroscopy shows preliminary promise as a less invasive method for identifying spectral features linked to HDRA-defined gemcitabine sensitivity in CCA. However, these findings do not establish clinical predictive utility and require validation in larger cohorts with comprehensive treatment-response data.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149129526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FTIR spectroscopy and chemometrics for monitoring fibrinogen-containing preparations during purification. FTIR光谱和化学计量学在纯化过程中监测含纤维蛋白原的制剂。

Biomolecules & biomedicine Pub Date : 2026-10-06 DOI: 10.17305/bb.2026.14830

Nikolay A Ryabov, Olga A Karyakina, Anastasiia A Surkova, Denis G Alekseev, Elena V Kudinova, Alisa A Klyuchnikova, Larisa T Volova

{"title":"FTIR spectroscopy and chemometrics for monitoring fibrinogen-containing preparations during purification.","authors":"Nikolay A Ryabov, Olga A Karyakina, Anastasiia A Surkova, Denis G Alekseev, Elena V Kudinova, Alisa A Klyuchnikova, Larisa T Volova","doi":"10.17305/bb.2026.14830","DOIUrl":"https://doi.org/10.17305/bb.2026.14830","url":null,"abstract":"Batch-to-batch variability in fibrinogen-containing plasma fractionation by-products complicates standardization, necessitating rapid process-monitoring tools. This proof-of-concept study explored Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA) to monitor a fibrinogen-containing preparation during processing. We analyzed raw precipitate, crude extract, and purified preparation from four independent production batches, alongside four commercial human fibrinogen lots, using FTIR spectroscopy and reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). PCA of spectra in the 1750-1107 cm⁻¹ region revealed four visually distinct clusters, with the first three principal components accounting for 99% of the total variance. Two additional final-product batches projected near the purified-preparation cluster. The purified preparation lacked a resolved feature at 1637-1639 cm⁻¹ and exhibited a dominant band at 1581-1582 cm⁻¹. SDS-PAGE demonstrated that the combined relative intensity of fibrinogen-associated Aα, Bβ, and γ bands increased from 64.6 ± 6.2% in raw precipitate to 82.5 ± 3.4% after purification, indicating fibrinogen enrichment rather than absolute purity. Due to differences in sampling configuration and matrix, spectral interpretation was limited to empirical fingerprints rather than direct measures of purity, aggregation, or secondary structure. These preliminary findings support further development of FTIR spectroscopy with PCA for process fingerprinting. However, routine implementation requires harmonized sampling, a larger number of independent production batches, predefined acceptance criteria, and external validation.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149129515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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