Biomolecules & biomedicine最新文献

{"title":"Hantaviruses in Southeast Europe: Climate change, rodent reservoirs, and One Health challenges.","authors":"Jurica Arapović, Luka Laura, Maja Arapović","doi":"10.17305/bb.2026.14499","DOIUrl":"https://doi.org/10.17305/bb.2026.14499","url":null,"abstract":"<p><p>Hantaviruses are not emerging pathogens in the strict sense, but their public health relevance is being reshaped by climate change, environmental disruption, land-use change, and increasing human mobility. In Southeast Europe, where Dobrava-Belgrade virus and Puumala virus remain endemic, these infections should no longer be viewed only as sporadic rodent-borne diseases of rural or forested environments. Changing ecological conditions can alter reservoir abundance, virus circulation, and human exposure, while fragmented surveillance and variable diagnostic capacity may obscure the true burden of disease. Although human infection is still driven primarily by environmental exposure to infected rodent excreta, rare person-to-person transmission of Andes virus and recent travel-associated clusters illustrate how traditionally localized zoonoses can acquire wider international relevance. This Opinion argues that hantaviruses should be approached as a climate-sensitive One Health challenge, requiring closer integration of human, veterinary, ecological, and meteorological surveillance. Strengthening regional preparedness is essential before environmental change further expands the conditions for transmission.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148297476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coronary artery calcium and multiparametric cardiovascular risk assessment in non-dialysis chronic kidney disease.","authors":"Ivana Đuran, Nikola Komazec, Bojana Ljubičić, Ana Lazarević, Siniša Živković, Tijana Azaševac, Sonja Golubović, Mia Manojlović","doi":"10.17305/bb.2026.14105","DOIUrl":"10.17305/bb.2026.14105","url":null,"abstract":"<p><p>Cardiovascular disease is a leading cause of mortality in chronic kidney disease (CKD), but whether impaired renal function independently increases coronary artery calcium (CAC) burden in patients with comparable cardiovascular referral indications remains uncertain. This retrospective cross-sectional study included 206 adults who underwent coronary computed tomography angiography (CCTA) with simultaneous Agatston scoring at the Institute for Cardiovascular Diseases of Vojvodina between 2020 and 2025. Patients were classified as having eGFR-defined non-dialysis CKD stages 3-5 (n=110; estimated glomerular filtration rate [eGFR] ≤59 mL/min/1.73 m²) or preserved renal function (n=96; eGFR ≥60 mL/min/1.73 m²). Both groups were drawn from the same cardiovascular referral population. The primary outcome was total Agatston score, while secondary analyses included Agatston severity categories, significant vessel-level stenosis, calcified coronary segment count, eGFR-Agatston correlation, and predictors of severe CAC. Total Agatston scores did not differ significantly between CKD and control groups (median 248.5 vs. 198.0; p=0.240), and severe calcification was similarly frequent (40.0% vs. 41.6%). Vessel-level stenosis, calcified segment distribution, and the eGFR-Agatston correlation were also non-significant. In multivariable logistic regression adjusted for age, sex, hypertension, diabetes, and prior coronary artery bypass grafting (CABG), CKD was not independently associated with severe CAC. However, CKD patients showed a markedly more adverse cardiovascular phenotype, including more hypertension, angina, peripheral arterial disease, prior CABG, lower left ventricular ejection fraction (LVEF), higher right ventricular systolic pressure (RVSP), and more severe valvular disease. These findings suggest that CAC scoring alone does not capture the multidimensional cardiovascular burden of non-dialysis CKD and should be integrated with echocardiographic and systemic vascular assessment in high-risk referral populations.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2360-2371"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of preoperative mGPS, CAR, and NLR in patients undergoing resection for non-small cell lung cancer.","authors":"Mehmet Mermer, Hıdır Esme","doi":"10.17305/bb.2026.14295","DOIUrl":"10.17305/bb.2026.14295","url":null,"abstract":"<p><p>Systemic inflammation is increasingly recognized as a crucial prognostic factor in non-small cell lung cancer (NSCLC). However, comprehensive evaluations comparing multiple inflammation-based indices in surgically treated cohorts, especially across specific time-dependent survival endpoints, remain scarce. This retrospective observational cohort study analyzed 270 NSCLC patients who underwent surgical resection of the primary tumor within the Konya City Hospital healthcare network. Preoperative inflammatory indices calculated included C-reactive protein to albumin ratio (CAR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), platelet-to-albumin ratio (PAR), and modified Glasgow Prognostic Score (mGPS). We assessed overall survival and time-specific survival at 1, 2, and 5 years. Group comparisons were conducted based on survival status at each time point, utilizing univariate and multivariable logistic regression to identify factors associated with time-specific survival. The mean age of the cohort was 71.64 ± 9.71 years, with 78.9% being male. Lobectomy was the most frequent procedure (72.6%). During follow-up, 145 patients died (overall mortality rate of 53.7%). Among these, the mean time from surgery to death was 32.63 ± 23.83 months. Survival rates at 1, 2, and 5 years were 90.0%, 79.3%, and 53.3%, respectively. Inflammatory indices were consistently elevated in non-survivors across all evaluated time points. In multivariable models, CAR and high mGPS status were independently associated with reduced 1-year survival. At 2 years, CAR and high mGPS status maintained their independent association with diminished survival, while NLR demonstrated borderline significance. For 5-year survival, high mGPS status and NLR remained independently associated with reduced survival. Unadjusted analyses revealed a graded association between mGPS and survival. Our findings indicate that preoperative systemic inflammation, particularly elevated mGPS status, CAR, and NLR, is associated with mortality at multiple postoperative time points following NSCLC resection. These results underscore the potential prognostic utility of readily available inflammation-based biomarkers and necessitate further validation through prospective investigations.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2285-2298"},"PeriodicalIF":0.0,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of <i>BRCA1 </i>variants of uncertain significance using ancestry-stratified allele frequencies: A Middle East-focused analysis.","authors":"Lama Abujamous, Rozaimi Razali, Hamda Al-Thawadi","doi":"10.17305/bb.2026.14033","DOIUrl":"10.17305/bb.2026.14033","url":null,"abstract":"<p><p>Interpreting BRCA1 variants of uncertain significance (VUS) in underrepresented populations presents significant challenges due to the scarcity of ancestry-matched reference data, which can lead to an overestimation of variant rarity. This study evaluates the effectiveness of employing ancestry-stratified allele frequencies, including a subset from the Middle East, within a calibrated ACMG/AMP framework to enhance the interpretation of BRCA1 VUS. We analyzed 626 BRCA1 variants from ClinVar, comprising 608 missense, 15 splice-altering, and 3 nonsense variants categorized as VUS or \"not provided.\" Variants were annotated using gnomAD v4 (both global and ancestry-stratified subsets) and dbNSFP, and subsequently evaluated according to workflows aligned with ACMG/AMP and ClinGen ENIGMA BRCA1/2 guidelines, with cross-validation performed using the Bayesian point system. Our findings revealed that ten variants (1.6%) were reclassified. Nine missense variants were downgraded to benign or likely benign status based on BS1 and BP1 criteria; notably, none of these downgrades would have occurred if evaluated solely on global gnomAD allele frequencies. Among these, five downgrades relied on the Middle East subset, while four depended on other ancestry-stratified subgroups. Additionally, one splice-altering variant (rs1555582723) was upgraded to likely pathogenic status based on PVS1_Very Strong and PM2_Supporting criteria. In conclusion, utilizing ancestry-aware population data modestly improved the resolution of BRCA1 VUS, primarily through benign downgrades. This underscores the necessity for larger regional datasets and further functional or case-based evidence to enhance variant interpretation.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2407-2418"},"PeriodicalIF":0.0,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148266924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>SLC28A3 </i>expression and fludarabine-emergent autoimmune hemolytic anemia in chronic lymphocytic leukemia: A new player in an old equation.","authors":"Vojin Vukovic, Teodora Karan-Djurasevic, Darko Antic, Natasa Tosic, Irena Marjanovic, Branka Zukic, Ivana Grubisa, Tamara Bibic, Kristina Tomic Vujovic, Vladimir Otasevic, Sofija Kozarac, Jelena Ivanovic, Sonja Pavlovic, Biljana Mihaljevic","doi":"10.17305/bb.2026.14208","DOIUrl":"10.17305/bb.2026.14208","url":null,"abstract":"<p><p>Fludarabine treatment in patients with chronic lymphocytic leukemia (CLL) has been associated with immune dysregulation and the development of autoimmune hemolytic anemia (AIHA). Prior research indicates a potential correlation between the expression of the SLC28A3 gene, which encodes human concentrative nucleoside transporter 3 (responsible for fludarabine uptake), and treatment response. This retrospective study investigated the relationship between baseline SLC28A3 expression levels, quantified via reverse-transcriptase polymerase chain reaction (qRT-PCR), and the incidence of AIHA in a cohort of CLL patients receiving fludarabine and cyclophosphamide (FC) therapy. Patients were categorized into two groups: \"FC-emergent AIHA,\" encompassing patients who developed AIHA during or following FC therapy, and \"no FC-emergent AIHA,\" which included patients with pre-existing AIHA prior to any treatment and those who remained AIHA-free throughout follow-up. A comparative analysis of these groups revealed no significant differences in most clinical and biological variables. However, a statistically significant difference was observed in median baseline SLC28A3 expression, which was sevenfold lower in the \"FC-emergent AIHA\" group compared to the \"no FC-emergent AIHA\" group. Furthermore, SLC28A3 expression did not significantly correlate with most of the other clinical or biological parameters, including time to first treatment or overall survival. These findings suggest that diminished baseline SLC28A3 expression may correlate with an elevated risk of fludarabine-emergent AIHA in CLL patients. Further validation through larger, prospective cohorts is essential to confirm the predictive utility of SLC28A3 expression for optimizing fludarabine-based therapeutic strategies.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2261-2271"},"PeriodicalIF":0.0,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148266966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter regarding \"Maternal smoking during pregnancy and risk of childhood-onset type 1 diabetes in offspring: A systematic review and meta-analysis\".","authors":"Neha Gangane, Seshadri Reddy Varikasuvu","doi":"10.17305/bb.2026.14445","DOIUrl":"10.17305/bb.2026.14445","url":null,"abstract":"<p><p>This correspondence discusses the recent meta-analysis by Zhang et al. examining maternal smoking during pregnancy and the risk of childhood-onset type 1 diabetes in offspring. The reported inverse association aligns with findings from previous meta-analyses and family-based epidemiological studies. However, interpreting this association remains challenging because observational perinatal research is susceptible to residual confounding, selection effects, and collider bias. Maternal smoking is closely linked with socioeconomic and behavioral factors that may independently influence immune development and autoimmune susceptibility in offspring. Mechanistic evidence also remains inconsistent, as experimental and developmental studies have generally associated prenatal tobacco exposure with adverse immune and metabolic outcomes rather than sustained autoimmune protection. The correspondence further highlights the absence of a formal GRADE certainty assessment and emphasizes that statistical precision should not be interpreted as evidence of causality. Further studies utilizing biomarker-confirmed exposure assessment, Mendelian randomization, and other causal inference approaches are warranted.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum phoenixin-14 and phoenixin-20 concentrations in polycystic ovary syndrome: Associations with anti-Müllerian hormone and diagnostic utility.","authors":"Oznur Dundar Akin, Naile Fevziye Misirlioglu, Seyma Dumur, Mete Hakan Karalok, Yeliz Cecen Donmez, Gonul Simsek, Hafize Uzun","doi":"10.17305/bb.2026.14394","DOIUrl":"10.17305/bb.2026.14394","url":null,"abstract":"<p><p>Polycystic ovary syndrome (PCOS) is a complex metabolic disorder characterized by altered peptide signaling that may contribute to reproductive dysfunction, insulin resistance, and metabolic dysregulation. This case-control study investigated serum phoenixin (PNX) isoforms, phoenixin-14 (PNX-14) and phoenixin-20 (PNX-20), in women with PCOS to evaluate their associations with biochemical and hormonal markers, including anti-Müllerian hormone (AMH), and assess their diagnostic utility. Serum PNX-14 and PNX-20 levels were measured in 90 women with PCOS and 60 age-matched healthy controls. Demographic, metabolic, and hormonal parameters were compared between the groups. Statistical analyses included receiver operating characteristic (ROC) analysis, logistic regression, correlation analysis, principal component analysis (PCA)-based clustering, mediation analysis, and exploratory Random Forest analysis. Results indicated that PNX-14 and PNX-20 levels were significantly higher in women with PCOS compared to controls (p < 0.001). Both isoforms correlated positively with AMH, insulin, homeostatic model assessment for insulin resistance (HOMA-IR), and luteinizing hormone (LH), while demonstrating negative correlations with sex hormone-binding globulin (SHBG) and high-density lipoprotein (HDL) cholesterol. ROC analysis showed moderate discriminatory performance, with area under the curve (AUC) values of 0.834 for PNX-14 and 0.819 for PNX-20. Mediation analysis suggested an associative pattern among PNX isoforms, AMH, and PCOS status. PCA-based clustering identified two distinct phenotypic profiles. Random Forest analysis ranked AMH, SHBG, fasting insulin, and body mass index (BMI) as the most influential variables, with PNX isoforms demonstrating lower importance scores. In conclusion, circulating PNX-14 and PNX-20 were elevated in PCOS and associated with key reproductive and metabolic parameters, suggesting a potential complementary role within multi-marker assessment frameworks. However, given the cross-sectional design and exploratory nature of the analyses, these findings should be considered hypothesis-generating rather than causal. Future longitudinal, mechanistic, and externally validated multicenter studies are essential to further elucidate these relationships.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2272-2284"},"PeriodicalIF":0.0,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13668010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HCV RNA detection in hepatic and extrahepatic tissues after DAA-induced sustained virologic response.","authors":"Samah Mamdouh, Hussam Hamdy, Tarek Aboushousha, Mona Magdy, Gehan Hammad, Mohamed Shemis, Amr Abdelraouf","doi":"10.17305/bb.2026.13329","DOIUrl":"10.17305/bb.2026.13329","url":null,"abstract":"<p><p>The complete eradication of hepatitis C virus (HCV) from both hepatic and extrahepatic compartments following successful direct-acting antiviral (DAA) therapy remains an area of ongoing investigation. This study aimed to detect HCV RNA signals in serum and tissue samples from 40 patients who had achieved a sustained virologic response (SVR). Paired serum samples (pre-treatment and post-SVR) were analyzed concurrently with liver, gallbladder, bile, pancreatic, and splenic tissues. These tissue samples were obtained during clinically indicated surgical procedures performed 4-12 weeks after SVR. HCV RNA was quantified using a real-time PCR assay, adapted exploratorily for tissue matrices. Statistical analysis encompassed paired comparisons for serum samples and descriptive analysis for tissue findings. All patients exhibited complete clearance of serum HCV RNA post-treatment (p < 0.001). Nevertheless, HCV RNA signals were detected in 11 of 40 liver tissue samples (27.5%) and in 6 of 40 gallbladder and bile samples (15%). Furthermore, detection was observed in the sole pancreatic sample (1/1) and in two of three splenic samples (2/3). Due to the constrained sample sizes, the extrahepatic findings are presented descriptively. Viral load measurements varied across tissues and are reported as median values with interquartile ranges. In conclusion, HCV RNA signals were detected in hepatic and selected extrahepatic tissues despite undetectable serum PCR following DAA therapy. It is crucial to note that these findings represent molecular detection exclusively and do not confirm active viral replication or persistence. These results should be considered exploratory and necessitate validation through strand-specific assays and larger patient cohorts.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2234-2242"},"PeriodicalIF":0.0,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a predictive risk model for post-stroke deep vein thrombosis.","authors":"Baichen Wang","doi":"10.17305/bb.2026.14312","DOIUrl":"10.17305/bb.2026.14312","url":null,"abstract":"<p><p>Deep vein thrombosis (DVT) represents a common and potentially fatal complication following acute ischemic stroke (AIS). However, early risk stratification is often difficult due to the asymptomatic nature of post-stroke DVT and the scarcity of AIS-specific predictive instruments. This study aimed to develop a nomogram utilizing baseline patient characteristics to forecast DVT risk after AIS. In this retrospective study, 848 AIS patients were analyzed, with 79 subsequently developing DVT. Least Absolute Shrinkage and Selection Operator (LASSO) regression and multivariable logistic regression identified five independent predictors: age (odds ratio [OR] = 1.074), fibrinogen (OR = 1.700), D-dimer (OR = 1.123), platelet count (OR = 1.001), and sex (OR = 0.475). A predictive nomogram was then constructed based on these variables. The model achieved an initial area under the receiver operating characteristic curve (AUC) of 0.809 and a Brier score of 0.074. Internal validation, conducted via 1,000-resample bootstrapping, yielded a stable optimism-corrected AUC of 0.800. Calibration plots demonstrated excellent concordance between predicted probabilities and observed outcomes. Decision curve analysis (DCA) indicated a positive net clinical benefit across a threshold probability range of 1%-91%, encompassing the clinically relevant 5%-20% decision window. In conclusion, this study successfully developed an exploratory nomogram for the early estimation of DVT risk in patients with AIS.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2328-2334"},"PeriodicalIF":0.0,"publicationDate":"2026-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148254600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroportin and soluble transferrin receptor in women with first-trimester missed abortion.","authors":"Ana Cuk, Lada Rumora, Ivanka Mikulic, Nikolina Penava, Ivona Cvetkovic, Vinka Mikulic, Kristina Ljubic, Ante Pusic, Andrea Prce, Dejan Tiric, Vajdana Tomic","doi":"10.17305/bb.2026.14261","DOIUrl":"10.17305/bb.2026.14261","url":null,"abstract":"<p><p>Laboratory diagnostics of iron homeostasis during pregnancy typically rely on routine parameters, which often inadequately reflect the actual maternal iron status. This study aimed to evaluate the investigative and diagnostic utility of soluble transferrin receptor (sTfR) and ferroportin (Fpn) concentrations as key indicators of iron dysregulation in women with normal early pregnancy and those experiencing missed abortion. These primary biomarkers were assessed in conjunction with standard laboratory parameters of iron status across the study groups. Notably, Fpn concentration had not previously been determined in women with either normal or abnormal pregnancies. The study enrolled 45 women with missed abortion and 45 women with normal first-trimester pregnancies. Peripheral blood concentrations of iron (Fe), unsaturated iron-binding capacity (UIBC), ferritin, hemoglobin (Hb), transferrin, and sTfR were measured using automated analyzers, while hepcidin and Fpn were quantified using ELISA kits. Transferrin saturation (TSAT), the sTfR/log ferritin index (sTfR-F index), and the ferroportin to soluble transferrin receptor ratio (Fpn/sTfR) were calculated. In the missed abortion group, Fpn (p < 0.001), Fe (p = 0.008), TSAT (p = 0.018), and Fpn/sTfR (p < 0.001) were significantly decreased, whereas sTfR (p = 0.001) and the sTfR-F index (p = 0.003) were significantly increased compared to women with normal pregnancies. Proposed multiparameter models incorporating sTfR and Fpn, or sTfR, hepcidin, and Fpn, accurately classified over 80% of cases and demonstrated excellent area under the curve (AUC) values of approximately 0.9. These findings indicate that Fpn and sTfR may serve as valuable investigative biomarkers for identifying dysregulation of iron homeostasis in early pregnancy.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2243-2250"},"PeriodicalIF":0.0,"publicationDate":"2026-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148240670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}