Biomolecules & biomedicine最新文献_第9页

Research progress on melatonin, 5-HT, and orexin in sleep disorders of children with autism spectrum disorder. 自闭症谱系障碍儿童睡眠障碍中褪黑激素、5-羟色胺和奥曲肽的研究进展。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11182

Wenjun Ding, Yiran Xu, Wencong Ding, Qiongyan Tang, Bohao Zhang, Yangyang Yuan, Jian Jin

{"title":"Research progress on melatonin, 5-HT, and orexin in sleep disorders of children with autism spectrum disorder.","authors":"Wenjun Ding, Yiran Xu, Wencong Ding, Qiongyan Tang, Bohao Zhang, Yangyang Yuan, Jian Jin","doi":"10.17305/bb.2024.11182","DOIUrl":"10.17305/bb.2024.11182","url":null,"abstract":"Sleep disorders are among the common comorbidities of autism spectrum disorder (ASD), which not only affect the daily life and learning ability of children but may also exacerbate other symptoms of ASD, seriously impacting the quality of life of children and their families. Given this, understanding the neurobiological mechanisms of sleep disorders in children with ASD has significant research value for developing effective intervention strategies. Melatonin, 5-hydroxytryptamine (5-HT), and orexin are key neurotransmitters that regulate the sleep-wake cycle. Through in-depth analysis of the biological functions and regulatory pathways of these neurotransmitters, new perspectives may be provided for personalized treatment of sleep disorders in children with ASD. This article reviews the research progress on melatonin, 5-HT, and orexin in sleep disorders among children with ASD, focusing on exploring the mechanisms of these key neurotransmitters in sleep disorders of children with ASD and how they affect the sleep-wake cycle, providing a theoretical basis for improving the sleep quality of children with ASD.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"525-533"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting TXNIP for neuroprotection: A novel approach to reducing inflammation and promoting recovery in ischemic stroke. 靶向TXNIP神经保护：缺血性脑卒中减少炎症和促进恢复的新途径。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11366

Chongxin He, Yong Bao, Yong Xu, Jingjing Cheng, Xinxin Hu

{"title":"Targeting TXNIP for neuroprotection: A novel approach to reducing inflammation and promoting recovery in ischemic stroke.","authors":"Chongxin He, Yong Bao, Yong Xu, Jingjing Cheng, Xinxin Hu","doi":"10.17305/bb.2024.11366","DOIUrl":"10.17305/bb.2024.11366","url":null,"abstract":"Ischemic stroke often results in high mortality and significant disability. Current research primarily focuses on understanding neuroinflammation and cell death following a stroke to identify novel therapeutic targets. This study investigates the endothelial cell-specific role of Thioredoxin interacting protein (TXNIP) in ischemic stroke and its underlying molecular mechanisms both in vitro and in vivo. By targeting endothelial cells, we aim to determine how TXNIP knockdown promotes neuroprotection, enhances angiogenesis, and reduces inflammation post-stroke. In vitro, an oxygen-glucose deprivation (OGD) model using bEnd.3 cells simulated ischemic conditions. Cellular injury was evaluated through cell proliferation and angiogenesis assays, while dual immunofluorescence staining assessed ZO-1 and CD31 expression. Western blotting measured protein levels of TXNIP, nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), ASC, pro-caspase-1, and interleukin-1β (IL-1β). In vivo, a middle cerebral artery occlusion (MCAO) mouse model was employed to mimic ischemic stroke. Brain injury was evaluated using triphenyl tetrazolium chloride (TTC) and Nissl staining, and molecular changes in injury markers were assessed via Western blot analysis. In vitro, TXNIP knockdown promoted cell proliferation and angiogenesis, reduced inflammation, and decreased ZO-1 and CD31 fluorescence intensity. TXNIP knockdown also reversed OGD-induced upregulation of TXNIP, NLRP3, ASC, pro-caspase-1, and IL-1β. In vivo, TXNIP knockdown improved neurological recovery, reflected by lower Longa scores, increased Nissl body presence, and reduced infarct size. These findings suggest that TXNIP knockdown mitigates inflammation, enhances angiogenesis, and reduces cerebral damage following ischemic stroke. This provides valuable insights into potential endothelial cell-specific therapeutic strategies for stroke treatment.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"553-562"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A repeated strike loading organ culture model for studying compression-associated chronic disc degeneration. 用于研究与挤压相关的慢性椎间盘退变的重复撞击加载器官培养模型。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.10640

Baoliang Li, Xu Chen, Hongkun Chen, Fu Zhang, Jianfeng Li, Zhengya Zhu, Tao Tang, Manman Gao, Nianhu Li, Liang Ma, Zhiyu Zhou

{"title":"A repeated strike loading organ culture model for studying compression-associated chronic disc degeneration.","authors":"Baoliang Li, Xu Chen, Hongkun Chen, Fu Zhang, Jianfeng Li, Zhengya Zhu, Tao Tang, Manman Gao, Nianhu Li, Liang Ma, Zhiyu Zhou","doi":"10.17305/bb.2024.10640","DOIUrl":"10.17305/bb.2024.10640","url":null,"abstract":"Mechanical stress has been viewed as one of the key risk factors in accelerating the intervertebral disc degeneration process. The goal of the present study was to employ a repeated strike loading bovine caudal disc system to elucidate the pathophysiological impacts of cumulative mechanical stress on the disc. The discs in the model groups were subjected to two different mechanical stresses: one strike loading or repeated strike loading. The following indices were analyzed: histological morphology, glycosaminoglycan release, disc height, cell viability, apoptosis-related protein expression, and catabolism-related gene expression. Both mechanical stress modes induced degenerative changes in the discs by day 11, such as clefts and delamination of the annulus fibrosus; they increased glycosaminoglycan release. Cell viability was significantly decreased and catabolic gene expression was significantly up-regulated in the degenerative loading group and repeated strike loading group by day 9. These alterations remained evident in the annulus fibrosus tissue of the repeated strike loading group on day 11. Our data suggests that the repeated strike loading model adopted in this study could lead to degenerative changes in the disc organ model. Annulus fibrosus cells displayed a more noticeable response to mechanical stress damage and a slower recovery process, suggesting that the annulus fibrosus serves as a pivotal factor in disc degeneration due to mechanical stress injuries. The study also indicates that due to the gradual self-repair of intervertebral disc cells after injury, it is necessary to apply repeated strike loading on the disc at specific intervals when researching the repair of chronic disc injuries.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"708-719"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of action of exercise regulating intestinal microflora to improve spontaneous hypertension in rats. 运动调节肠道微生物菌群改善大鼠自发性高血压的作用机制。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11174

Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu

{"title":"Mechanism of action of exercise regulating intestinal microflora to improve spontaneous hypertension in rats.","authors":"Yu Li, Xiaoju Song, Lianjing Dai, Yangyi Wang, Qiong Luo, Lei Lei, Yunfei Pu","doi":"10.17305/bb.2024.11174","DOIUrl":"10.17305/bb.2024.11174","url":null,"abstract":"Hypertension is a prevalent cardiovascular disease. Exercise is widely recognized as an effective treatment for hypertension, and it may also influence the composition of the intestinal microflora. However, it remains unclear whether exercise can specifically regulate the intestinal microflora in the context of hypertension treatment. In this study, tail blood pressure in spontaneously hypertensive rats (SHR) was measured using a blood pressure meter after exercise intervention and fecal bacteria transplantation following exercise. Blood lipid levels were assessed using an automatic biochemical analyzer, and 16S rRNA sequencing was employed to analyze the intestinal microflora. Histological examinations of ileal tissue were conducted using HE and Masson staining. Intestinal permeability, inflammatory status, and sympathetic activity were evaluated by measuring the levels of diamine oxidase, D-lactic acid, C-reactive protein, interleukin-6, tumor necrosis factor-α, lipopolysaccharide, norepinephrine, angiotensin II, cyclic adenosine monophosphate, and cyclic guanosine monophosphate. Exercise was found to reduce blood pressure and blood lipid levels in SHR. It also improved the composition of the intestinal microflora, as evidenced by a reduced Firmicutes/Bacteroidetes ratio, an increase in bacteria that produce acetic and butyric acid, and higher Chao 1 and Shannon diversity indices. Furthermore, exercise reduced the thickness of the fibrotic and muscular layers in the ileum, increased the goblet cell/villus ratio and villus length, and decreased intestinal permeability, inflammatory markers, and sympathetic nerve activity. The intestinal microbial flora regulated by exercise demonstrated similar effects on hypertension. In conclusion, exercise appears to regulate the intestinal microflora, and this exercise-induced change in flora may contribute to improvements in hypertension in rats.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"648-662"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142559616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of simvastatin in ALS: Enhanced axonal integrity and motor neuron survival through Apoa4 and Alb modulation. 辛伐他汀对 ALS 的治疗潜力：通过调节 Apoa4 和 Alb 增强轴突完整性和运动神经元存活率

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11218

Song Luo, Xiaorui Wang, Bo Ma, Dongliang Liu, Li Li, Lijin Wang, Ning Ding, Liangyu Zou, Jie Wang, Jialin Pan, Daoqian Sang, Huadong Zhou, Hongdang Qu, Yi Lu, Lijuan Yang

{"title":"Therapeutic potential of simvastatin in ALS: Enhanced axonal integrity and motor neuron survival through Apoa4 and Alb modulation.","authors":"Song Luo, Xiaorui Wang, Bo Ma, Dongliang Liu, Li Li, Lijin Wang, Ning Ding, Liangyu Zou, Jie Wang, Jialin Pan, Daoqian Sang, Huadong Zhou, Hongdang Qu, Yi Lu, Lijuan Yang","doi":"10.17305/bb.2024.11218","DOIUrl":"10.17305/bb.2024.11218","url":null,"abstract":"Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the selective death of motor neurons in the spinal cord, brainstem, and motor cortex. This study investigates the effects of simvastatin on the G93A-copper/zinc superoxide dismutase (G93ASOD1) transgenic mouse model of ALS. The experiment included three groups: C57BL/6 wild-type mice, C57BL/6J SOD1G93A mice treated with PBS (SOD1G93A + PBS), and C57BL/6J SOD1G93A mice treated with simvastatin (SOD1G93A + simvastatin). The primary endpoints were survival rates, body weight changes, performance in pole climbing and suspension tests, and neurological deficit scores. Pathological changes were assessed using hematoxylin and eosin staining, transmission electron microscopy, Nissl staining, and Masson staining. Proteomic and metabolomic analyses were performed to identify differentially expressed proteins (DEPs) and metabolites. Quantitative real-time polymerase chain reaction and western blotting were used to measure gene expression. Although there were no significant differences in survival rates, body weight, pole climbing, and suspension test performance, or neurological deficit scores between the SOD1G93A + simvastatin and SOD1G93A + PBS groups, simvastatin treatment improved axonal organization within the spinal cord, increased the number of neurons, and reduced cytoplasmic swelling and gastrocnemius fibrosis. A total of 47 DEPs and 13 differential metabolites were identified between the SOD1G93A + PBS and SOD1G93A + simvastatin groups. Notably, the expression levels of Apoa4 and Alb were elevated in the SOD1G93A + simvastatin group compared to the SOD1G93A + PBS group. Our results suggest that simvastatin may have potential therapeutic effects in ALS, likely involving the modulation of Apoa4 and Alb expression.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"632-647"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathologic degenerative score as a predictor of minimal clinically important difference in pain and functionality following surgical treatment for disc herniation. 组织病理学退行性评分作为椎间盘突出症手术治疗后疼痛和功能性最小临床重要差异的预测指标。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.10877

Hakija Bečulić, Emir Begagić, Sabina Šegalo, Fatima Juković-Bihorac, Emsel Papić, Ragib Pugonja, Amina Džidić-Krivić, Adem Nuhović, Goran Lakičević, Semir Vranić, Mirza Pojskić

{"title":"Histopathologic degenerative score as a predictor of minimal clinically important difference in pain and functionality following surgical treatment for disc herniation.","authors":"Hakija Bečulić, Emir Begagić, Sabina Šegalo, Fatima Juković-Bihorac, Emsel Papić, Ragib Pugonja, Amina Džidić-Krivić, Adem Nuhović, Goran Lakičević, Semir Vranić, Mirza Pojskić","doi":"10.17305/bb.2024.10877","DOIUrl":"10.17305/bb.2024.10877","url":null,"abstract":"Lumbar disc herniation (LDH) often results in significant pain and disability, and histopathologic (HP) evaluation of intervertebral discs (IVDs) offers critical insights into treatment outcomes. This prospective observational study explores HP changes in IVDs and their association with clinical outcomes following surgical treatment for LDH. A cohort of 141 patients undergoing MRI-confirmed LDH surgery underwent HP evaluation using a semi-quantitative HP degeneration score (HDS). Preoperatively and at a six-month follow-up, the comprehensive clinical assessment included the Oswestry disability index (ODI) and visual analog scale (VAS), with a minimal clinically important difference (MCID) calculated from ODI and VAS. Results indicated significant associations between higher HDS and adverse clinical outcomes, including persistent pain and greater disability post-surgery. Specifically, an HDS ≥ 7 was predictive (OR = 6.25, 95% CI: 2.56-15.23) of disability outcomes measured with MCID-ODI (AUC: 0.692, 95% CI: 0.609-0.767, P < 0.001), and HDS ≥ 8 was predictive (OR = 1.72, 95% CI: 1.04-2.77) of persistent pain measured with MCID-VAS (AUC = 0.628, 95% CI: 0.598-0.737, P = 0.008), highlighting the diagnostic potential of HDS in assessing postoperative recovery. This study underscores the potential of HP evaluation using HDS to provide valuable insights into disease progression and outcomes in LDH patients, complementing conventional radiologic methods. The findings support the application of personalized treatment strategies based on HP findings while acknowledging challenges in interpretation and clinical implementation.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"623-631"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of parecoxib on postoperative cognitive function and analgesic safety in elderly patients undergoing gastrointestinal tumor resection: A retrospective study. 帕瑞昔布对接受胃肠道肿瘤切除术的老年患者术后认知功能和镇痛安全性的影响：一项回顾性研究。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11042

Yongli Li, Yan Peng

{"title":"Effect of parecoxib on postoperative cognitive function and analgesic safety in elderly patients undergoing gastrointestinal tumor resection: A retrospective study.","authors":"Yongli Li, Yan Peng","doi":"10.17305/bb.2024.11042","DOIUrl":"10.17305/bb.2024.11042","url":null,"abstract":"Neuroinflammation is associated with the development of postoperative cognitive dysfunction (POCD). Parecoxib has powerful anti-inflammatory and analgesic effects, which may reduce the occurrence of POCD. We hypothesized that parecoxib could reduce the incidence of POCD and relieve postoperative pain without increasing postoperative complications in elderly patients with gastrointestinal cancer. The study analyzed the effect of parecoxib on elderly patients undergoing elective radical resection of gastrointestinal tumors. Patients were divided into the NSAIDs group and the non-NSAIDs group according to whether parecoxib was administered. Demographic and clinical data were collected and compared. The incidence of POCD was set as the primary outcome, and postoperative pain as the secondary outcome. Among the 440 enrolled patients, the POCD incidence rates within 7 days after surgery in the NSAIDs and non-NSAIDs groups were 42.60% and 40.30%, respectively, with no statistically significant difference (P > 0.05). Patients in the NSAIDs group experienced significantly less pain on the first and second days after surgery compared to the non-NSAIDs group (P < 0.05). There were no statistically significant differences in postoperative adverse events between the two groups (P > 0.05). Parecoxib had no significant negative effect on early postoperative cognitive function, effectively alleviating early postoperative acute pain without increasing postoperative complications. The findings have implications for the broader use of parecoxib in postoperative pain management in elderly patients undergoing major surgery.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"720-726"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin ameliorates ischemic stroke injury by downregulating GMFB expression: An in vitro study. 姜黄素通过下调 GMFB 的表达改善缺血性中风损伤：一项体外研究。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.10957

Xiumei Bai, Yabin Song, Xiangyan Zhang, Liqiong Liu, Haixia Wu, Jiaqing Feng, Lihong Wu, Huizhen Liu, Diangui Zhou

{"title":"Curcumin ameliorates ischemic stroke injury by downregulating GMFB expression: An in vitro study.","authors":"Xiumei Bai, Yabin Song, Xiangyan Zhang, Liqiong Liu, Haixia Wu, Jiaqing Feng, Lihong Wu, Huizhen Liu, Diangui Zhou","doi":"10.17305/bb.2024.10957","DOIUrl":"10.17305/bb.2024.10957","url":null,"abstract":"Ischemic stroke (IS) is a cerebrovascular sickness, and cerebral ischemia-reperfusion (I/R) damage often occurs, but there is still a lack of drugs that can significantly alleviate it. Curcumin (Cur) exerts pharmacological effects such as antioxidative stress, anti-inflammation, and the promotion of apoptosis through regulating various pathways, but its efficacy and specific mechanism of action in IS have not been fully clarified. The purpose of this paper is to study the influence of Cur on IS. Brain microvascular endothelial cells (BMECs) were used to create an oxygen-glucose deprivation/reoxygenation (OGD/R) model to simulate I/R damage. The cell viability was assessed using an MTT assay. The LDH level and ROS positive rate were measured using commercial kits. The cell invasion was examined using a transwell assay. The apoptosis was assessed by flow cytometry. The contents of GMFB, Bax, and Bcl2 were measured using western blot. We confirmed that in the OGD/R-induced IS cell model, the abundance of GMFB was enhanced in the OGD/R group versus the control group. GMFB overexpression promoted OGD/R-induced cell viability diminution, increased LDH and ROS levels, lessened cell invasion ability, enhanced cell apoptosis, enhanced Bax levels, and decreased Bcl2 levels. Silencing GMFB ameliorated OGD/R-induced cell damage. Cur ameliorated OGD/R-induced cell damage. Cur curbed OGD/R-induced cell damage by downregulating GMFB expression. In conclusion, Cur cured ischemic stroke-induced cell damage by downregulating GMFB expression.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"578-587"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway. PF4通过调节CXCR3/AKT1/SLC7A11信号通路抑制铁蛋白沉积介导的脑内出血。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11283

Na Hu, Guohong Zhang, Liping An, Wei Wang, Ran An, Yunfeng Li

{"title":"PF4 inhibits ferroptosis-mediated intracerebral hemorrhage through modulating the CXCR3/AKT1/SLC7A11 signaling pathway.","authors":"Na Hu, Guohong Zhang, Liping An, Wei Wang, Ran An, Yunfeng Li","doi":"10.17305/bb.2024.11283","DOIUrl":"10.17305/bb.2024.11283","url":null,"abstract":"Ferroptosis plays a crucial role in the secondary pathophysiological damage to brain tissue surrounding hematomas after intracerebral hemorrhage (ICH). While platelet factor 4 (PF4) is known to promote regeneration following peripheral nerve injury, its role in brain tissue repair after cerebral hemorrhage remains unclear. In this study, Hemin-induced PC12 cells were treated with various inhibitors and assessed for viability, oxidative stress, and ferroptosis using a combination of assays, including CCK-8 (Cell Counting Kit-8), EdU (5-Ethynyl-2'-deoxyuridine), flow cytometry, and immunofluorescence. ICH cells were also treated with recombinant PF4 (Rm-PF4) and a CXCR3 antagonist (AMG487) to investigate the mechanism by which Rm-PF4 influences Hemin-induced PC12 cell injury and inflammation. Subsequently, ICH mouse models were established via collagenase injection. Neurological function in these mice was evaluated using the Cylinder and Corner tests. Histopathological damage to brain tissue was analyzed through HE, TUNEL, and Nissl staining, as well as immunohistochemistry, to further explore the role of Rm-PF4 in controlling neuroinflammation in vivo. Results showed that Rm-PF4 inhibited Hemin-mediated ferroptosis-induced PC12 cell damage and inflammation by activating the CXCR3/AKT1/SLC7A11 signaling pathway. Blocking the CXCR3/AKT1/SLC7A11 pathway partially reversed PF4's protective effects on Hemin-induced PC12 cells.In ICH mice, pro-inflammatory marker CD16 (3rd day) and anti-inflammatory marker Arg1 (7th day) were significantly decreased and increased, respectively (p<0.05). IL-6, TNF-α, and IL-1β levels were down-regulated in brain tissues after Rm-PF4 injection, which was significantly reversed by AMG487. PF4 inhibits ferroptosis after ICH reduced PC12 cell damage and the inflammatory response via activating the CXCR3/AKT1/SLC7A11 pathway.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"563-577"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142670083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined sonographic optic nerve sheath diameter and cerebral oximeter for predicting neurological outcome after cardiac arrest. 结合超声视神经鞘直径和脑氧化仪预测心脏骤停后的神经功能预后。

Biomolecules & biomedicine Pub Date : 2025-01-30 DOI: 10.17305/bb.2024.11442

Mehmet Akif Yazar, Betul Kozanhan, Yasin Tire, Nevin Sekmenli, Guzide Yazar, Murat Sevim

{"title":"Combined sonographic optic nerve sheath diameter and cerebral oximeter for predicting neurological outcome after cardiac arrest.","authors":"Mehmet Akif Yazar, Betul Kozanhan, Yasin Tire, Nevin Sekmenli, Guzide Yazar, Murat Sevim","doi":"10.17305/bb.2024.11442","DOIUrl":"10.17305/bb.2024.11442","url":null,"abstract":"Cardiac arrest (CA) remains a critical global health issue with high rates of mortality and morbidity. Accurate prediction of neurological outcomes in post-CA patients is essential for optimizing management strategies. Optic nerve sheath diameter (ONSD) and near-infrared spectroscopy (NIRS) are emerging as promising tools for evaluating brain oxygenation and intracranial pressure. However, the potential benefits of combining these methods for improved prognostic accuracy have not been thoroughly explored. This study investigates whether the combined use of ultrasonographic ONSD and NIRS measurements enhances the prediction of neurological outcomes after CA. In this prospective study, ONSD measurements were obtained three times at 24-hour intervals, while regional hemoglobin oxygen saturation (rSO2) using NIRS was recorded twice. Neurological outcomes were assessed using the Full Outline of Unresponsiveness (FOUR) and Cerebral Performance Categories (CPC) scores for both early and late evaluations. Results indicated that 47.5% of patients had poor outcomes and 52.5% had good outcomes based on the FOUR score, while 65% had poor outcomes and 35% had good outcomes according to the CPC score. The combination of ONSD and NIRS measurements showed superior prognostic performance compared to either method alone. While standalone NIRS measurements taken after 24 hours exhibited limited predictive value, combining ONSD and NIRS provided a more reliable approach for neurological assessment in the short term following CA. This integrated method may improve prognostic accuracy and support better clinical decision-making.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"672-681"},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0