Biomolecules & biomedicine最新文献_第2页

Is SARS-CoV-2 facing constraints in its adaptive evolution? SARS-CoV-2的适应性进化是否面临制约？

Biomolecules & biomedicine Pub Date : 2025-06-09 DOI: 10.17305/bb.2025.12537

Yingguang Liu

{"title":"Is SARS-CoV-2 facing constraints in its adaptive evolution?","authors":"Yingguang Liu","doi":"10.17305/bb.2025.12537","DOIUrl":"https://doi.org/10.17305/bb.2025.12537","url":null,"abstract":"The ultimate measure of viral fitness is the ability to maintain high prevalence within its host species. Effective transmission, efficient replication, and rapid immune evasion all contribute to this outcome. Over the past five years, SARS-CoV-2 has successfully adapted to humans, establishing long-term reservoirs and enabling sustained coexistence with the human population. We have observed innovative, synergistic mutations in the spike (S) protein that enhance receptor binding. Adaptation to the upper respiratory tract has shortened the incubation period, thereby facilitating viral spread. These improvements have also enabled immune escape mutations, even when such changes compromise replicative fitness. Adaptive mutations have driven intermittent selective sweeps by dominant variants. However, there are limits to functional enhancement. The receptor binding affinity of the S protein appears to have peaked between 2022 and 2023. The accumulation of fixed mutations plateaued following the emergence of BA.2.86/JN.1 around late 2023 and early 2024. Purifying selection has been the dominant evolutionary force acting on nonsynonymous mutations in the Omicron lineage, and the overall fitness impact of missense mutations in key viral proteins has declined. Additionally, due to weak selection pressure on synonymous mutations, the codon adaptation index in humans has been decreasing among Omicron subvariants. As a result, Omicron lineages have replicated less efficiently in cell cultures compared to the original virus, and recent variants show further attenuation in animal models. In the human population, this attenuation is reflected in declining COVID-19-related mortality, despite persistently high infection rates.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OncoImmune machine-learning model predicts immune response and prognosis in leiomyosarcoma. OncoImmune机器学习模型预测平滑肌肉瘤的免疫反应和预后。

Biomolecules & biomedicine Pub Date : 2025-06-04 DOI: 10.17305/bb.2025.12342

Jingrong Deng, Changfa Shu, Dong Wang, Richard Nimbona, Xingping Zhao, Dabao Xu

{"title":"OncoImmune machine-learning model predicts immune response and prognosis in leiomyosarcoma.","authors":"Jingrong Deng, Changfa Shu, Dong Wang, Richard Nimbona, Xingping Zhao, Dabao Xu","doi":"10.17305/bb.2025.12342","DOIUrl":"https://doi.org/10.17305/bb.2025.12342","url":null,"abstract":"Leiomyosarcoma (LMS) is one of the most aggressive tumors originating from smooth muscle cells, characterized by a high recurrence rate and frequent distant metastasis. Despite advancements in targeted therapies and immunotherapies, these interventions have failed to significantly improve the long-term prognosis for LMS patients. Here, we identified OncoImmune differential expressed genes (DEGs) that influence monocytes differentiation and the progression of LMS, revealing varied immune activation states of LMS patients. Using a machine learning approach, we developed a prognostic model based on OncoImmune hub DEGs, which offers a moderate accuracy in predicting risk levels among LMS patients. Mechanistically, we found that ATRX mutation may regulate coiled-coil domain-containing protein 69 (CCDC69) expression, leading to functional alterations in mast cells and immune unresponsiveness through the modulation of various immune-related signaling pathways. This machine learning-based prognostic model, centered on seven OncoImmune hub DEGs, along with ATRX gene status, represents promising biomarkers for predicting prognosis, molecular characteristics, and immune features in LMS.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144267985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ending nuclear weapons, before they end us. 在核武器终结我们之前终结它们。

Biomolecules & biomedicine Pub Date : 2025-06-03 DOI: 10.17305/bb.2025.12736

Kamran Abbasi

引用次数: 0

Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes. CADPS低表达预示无融合基因的儿童急性淋巴细胞白血病预后不良。

Biomolecules & biomedicine Pub Date : 2025-05-30 DOI: 10.17305/bb.2025.12254

Bin Zhang, Chaoran Shi, Xiuxiu Wang, Jiajia Mi, Runan Wang, Shuang Li, Jiawei Yang, Qiuying He, Yujiao Wang, Zuofei Chi, Liangchun Hao

{"title":"Low expression of CADPS predicts poor prognosis in pediatric acute lymphoblastic leukemia without fusion genes.","authors":"Bin Zhang, Chaoran Shi, Xiuxiu Wang, Jiajia Mi, Runan Wang, Shuang Li, Jiawei Yang, Qiuying He, Yujiao Wang, Zuofei Chi, Liangchun Hao","doi":"10.17305/bb.2025.12254","DOIUrl":"https://doi.org/10.17305/bb.2025.12254","url":null,"abstract":"Pediatric acute lymphoblastic leukemia (ALL) is among the most prevalent hematological malignancies in children. Despite an overall cure rate approaching 90%, a subset of patients still experiences relapse, even with advanced therapeutic interventions. Research into the molecular characteristics and prognostic markers of fusion gene-negative (FG-negative) pediatric ALL remains limited. To address this gap, we performed whole-exome sequencing (WES) and whole-transcriptome sequencing (RNA-seq) on 54 FG-negative ALL cases from our center. Our results indicated that neither specific mutations nor tumor mutational burden significantly influenced relapse risk. Notably, we identified a significant downregulation of CADPS in FG-negative pediatric ALL patients who relapsed. The expression levels and prognostic significance of CADPS were further validated using data from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) cohort, where lower CADPS expression was associated with reduced event-free survival (EFS) and overall survival (OS) (P < 0.001 for both). Cox regression analyses were subsequently employed to identify OS-related factors and to construct a prognostic prediction model. Notably, this model demonstrated a significant correlation with therapeutic targets. In conclusion, our findings support the potential of CADPS expression as a novel biomarker for prognostic stratification in FG-negative pediatric ALL patients.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of menopause status on T-helper cell profiles in acute myocardial infarction. 绝经状态对急性心肌梗死患者t辅助细胞谱的影响。

Biomolecules & biomedicine Pub Date : 2025-05-28 DOI: 10.17305/bb.2025.12354

Fernanda Espinosa-Bautista, Varna Ramos-Rosillo, Yadira V Ázquez-Panchos, Fernanda Bocanegra-Zamora, Héctor González-Pacheco, Mariana Patlán, Araceli Páez, Felipe Massó, Luis M Amezcua-Guerra

{"title":"Influence of menopause status on T-helper cell profiles in acute myocardial infarction.","authors":"Fernanda Espinosa-Bautista, Varna Ramos-Rosillo, Yadira V Ázquez-Panchos, Fernanda Bocanegra-Zamora, Héctor González-Pacheco, Mariana Patlán, Araceli Páez, Felipe Massó, Luis M Amezcua-Guerra","doi":"10.17305/bb.2025.12354","DOIUrl":"https://doi.org/10.17305/bb.2025.12354","url":null,"abstract":"Estrogens modulate immune responses, particularly the activation and polarization of CD4+ T cells, which play key roles in cardiovascular homeostasis. This proof-of-concept study investigated the effect of menopausal status on the polarization of T-helper (Th) cells in women with acute myocardial infarction (AMI). A total of 41 female AMI patients were enrolled-seven premenopausal and 34 postmenopausal-and compared with a group of 17 male AMI patients. Flow cytometry was used to evaluate CD4+ T-cell subsets, including Th1 (T-bet+), Th2 (GATA3+), and Th17 (RORγt+) phenotypes. Serum levels of representative cytokines were also measured. Women exhibited higher numbers of circulating CD4+ T cells compared to men, with a marked shift toward the Th1 phenotype. Postmenopausal women demonstrated increased cardiovascular risk, as indicated by higher QRISK3 and GRACE scores, as well as elevated levels of C-reactive protein and cardiac troponin T compared to premenopausal women. However, menopausal status had minimal impact on Th cell polarization, as no significant differences were observed in the proportions of Th1, Th2, or Th17 subsets between premenopausal and postmenopausal women. Similarly, levels of interleukin (IL)-6, IL-1β, IL-10, tumor necrosis factor, and monocyte chemoattractant protein-1 were comparable between the two groups. This proof-of-concept study highlights sex-specific differences in immune responses and inflammatory profiles during AMI. Women exhibited a stronger polarization toward the Th1 phenotype, along with elevated markers of inflammation and myocardial injury. Notably, menopausal status did not significantly affect lymphocyte subpopulations or circulating cytokine levels.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical significance of a novel inflammatory-nutritional index in glaucoma severity evaluation. 一种新的炎症-营养指数在青光眼严重程度评价中的临床意义。

Biomolecules & biomedicine Pub Date : 2025-05-27 DOI: 10.17305/bb.2025.12374

Xiao Xiao, Yanping Gao, Gao Zhang, Zuo Wang, An Li, Donghua Liu, Jing Fu, Wenbo Xiu, Chang Lu, Jinxia Wang, Xiong Zhu, Yang Chen, Lingling Chen, Bolin Deng, Ping Shuai, Chong He, Fang Lu

{"title":"Clinical significance of a novel inflammatory-nutritional index in glaucoma severity evaluation.","authors":"Xiao Xiao, Yanping Gao, Gao Zhang, Zuo Wang, An Li, Donghua Liu, Jing Fu, Wenbo Xiu, Chang Lu, Jinxia Wang, Xiong Zhu, Yang Chen, Lingling Chen, Bolin Deng, Ping Shuai, Chong He, Fang Lu","doi":"10.17305/bb.2025.12374","DOIUrl":"https://doi.org/10.17305/bb.2025.12374","url":null,"abstract":"This study investigated the association between glaucoma and serum albumin (Alb), lymphocyte percentage (LYMPH%), and their combined index (LAP = LYMPH% × Alb), to evaluate their potential as biomarkers for systemic inflammation and disease progression in glaucoma. We enrolled 161 glaucoma patients and 181 healthy controls. Serum Alb and LYMPH% were measured using standard blood biochemistry and routine tests, and LAP was calculated accordingly. Statistical analyses were performed to compare these markers between groups and assess their correlation with disease severity. Both the median serum Alb level and peripheral blood LYMPH% were significantly lower in the glaucoma group compared to controls (Alb: 43.48 g/L vs 44.63 g/L, P < 0.001; LYMPH%: 24.25% vs 29.12%, P < 0.001). Correspondingly, LAP levels were also significantly reduced in glaucoma patients (1053 vs 1298, P < 0.001). Lower LYMPH% and LAP levels were associated with more severe glaucomatous visual impairment (LAP, healthy controls vs glaucoma: AUC = 0.7080, P < 0.001, Max Youden = 0.3621; early vs severe glaucoma: AUC = 0.8061, P < 0.001, Max Youden = 0.5377). In summary, LAP may serve as a supportive biomarker of systemic inflammation in glaucoma. It demonstrates good accuracy in reflecting glaucoma severity and shows potential for monitoring disease progression.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of COPD in survival of NSCLC patients receiving immune checkpoint inhibitors: A meta-analysis. COPD在接受免疫检查点抑制剂的非小细胞肺癌患者生存中的作用：一项荟萃分析。

Biomolecules & biomedicine Pub Date : 2025-05-26 DOI: 10.17305/bb.2025.12355

Yan Zhu, Chongyang Wang

{"title":"The role of COPD in survival of NSCLC patients receiving immune checkpoint inhibitors: A meta-analysis.","authors":"Yan Zhu, Chongyang Wang","doi":"10.17305/bb.2025.12355","DOIUrl":"https://doi.org/10.17305/bb.2025.12355","url":null,"abstract":"The impact of chronic obstructive pulmonary disease (COPD) on the survival of patients with non-small cell lung cancer (NSCLC) receiving immune checkpoint inhibitors (ICIs) remains unclear. Given the growing use of ICIs in NSCLC treatment and the high prevalence of COPD among these patients, understanding this relationship is essential. This meta-analysis aims to evaluate the association between COPD and survival outcomes in NSCLC patients treated with ICIs. A systematic search was conducted in PubMed, Embase, and Web of Science from inception to February 10, 2025. Observational studies reporting survival outcomes in NSCLC patients with and without COPD undergoing ICI therapy were included. Hazard ratios (HRs) with 95% confidence intervals (CIs) were pooled using a random-effects model to account for heterogeneity. Thirteen retrospective cohort studies involving 5,564 patients were included. COPD was associated with improved progression-free survival (PFS) (HR: 0.68, 95% CI: 0.54-0.85, p < 0.001) and overall survival (OS) (HR: 0.80, 95% CI: 0.68-0.95, p = 0.01) in NSCLC patients receiving ICIs. Heterogeneity was moderate (I² = 46% for PFS, I² = 43% for OS). Subgroup analyses indicated that the association between COPD and survival outcomes was consistent across study regions (Asian vs. Western countries), patient age, sex distribution, COPD diagnostic criteria (spirometry, clinical diagnosis, or CT-diagnosed emphysema), follow-up duration, analytic models (univariate vs. multivariate), and study quality scores (p for subgroup differences > 0.05). Furthermore, univariate meta-regression analysis showed no significant modification of results by sample size, mean age, sex distribution, follow-up duration, or study quality scores (all p > 0.05).","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The immunotherapy breakthroughs in cervical cancer: Focus on potential biomarkers and further therapy advances. 宫颈癌免疫治疗的突破：关注潜在的生物标志物和进一步的治疗进展。

Biomolecules & biomedicine Pub Date : 2025-05-22 DOI: 10.17305/bb.2025.12597

Maja Pezer Naletilić, Krešimir Tomić, Kristina Katić, Zoran Gatalica, Gordan Srkalović, Eduard Vrdoljak, Semir Vranić

{"title":"The immunotherapy breakthroughs in cervical cancer: Focus on potential biomarkers and further therapy advances.","authors":"Maja Pezer Naletilić, Krešimir Tomić, Kristina Katić, Zoran Gatalica, Gordan Srkalović, Eduard Vrdoljak, Semir Vranić","doi":"10.17305/bb.2025.12597","DOIUrl":"10.17305/bb.2025.12597","url":null,"abstract":"Despite the well-established role of human papillomavirus (HPV) as the primary cause of cervical cancer (CC) and the existence of an effective HPV vaccine, over half a million women are diagnosed with CC globally each year, with more than half of them dying from the disease. Immunotherapy has rapidly become a cornerstone of cancer treatment, offering substantial improvements in survival rates and reducing treatment-related side effects compared to traditional therapies. For the past 25 years, chemoradiotherapy (CRT) has been the standard treatment for locally advanced CC (LACC). However, while adjuvant chemotherapy has failed to improve outcomes in LACC, the integration of neoadjuvant chemotherapy (NACT) with CRT, as well as chemoimmunoradiotherapy followed by consolidation immunotherapy, has transformed treatment strategies, demonstrating superior efficacy compared to CRT alone. In the first-line treatment of CC, adding pembrolizumab to platinum-based chemotherapy, either with or without bevacizumab, has significantly improved outcomes compared to platinum-based chemotherapy and bevacizumab alone. This review explores the current landscape of immunotherapy and biomarker advancements in CC. Furthermore, we discuss promising future directions, including the potential of personalized immunotherapy approaches and novel combination therapies to further enhance treatment efficacy and improve prognoses for patients with CC.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144133065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the link between environmental chemical exposures and epigenetic modifications in diabetes mellitus. 探讨环境化学物质暴露与糖尿病表观遗传修饰之间的联系。

Biomolecules & biomedicine Pub Date : 2025-05-21 DOI: 10.17305/bb.2025.11801

Tasnim R Matarid, Menatallah Rayan, Ola J Hussein, Hanan H Abunada, Zaid H Maayah, Hesham M Korashy

{"title":"Exploring the link between environmental chemical exposures and epigenetic modifications in diabetes mellitus.","authors":"Tasnim R Matarid, Menatallah Rayan, Ola J Hussein, Hanan H Abunada, Zaid H Maayah, Hesham M Korashy","doi":"10.17305/bb.2025.11801","DOIUrl":"https://doi.org/10.17305/bb.2025.11801","url":null,"abstract":"Diabetes mellitus (DM) is a globally prevalent metabolic disorder characterized by impaired glucose homeostasis and insulin secretion. Beyond traditional risk factors like lifestyle and genetics, environmental pollutants, including particulate matter, heavy metals, and persistent organic pollutants, have become significant contributors to DM. One of the key mechanistic pathways through which these pollutants exert their effects is the activation of the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor that regulates the expression of cytochrome P450 family 1 (CYP1) enzymes. This cascade contributes to increased oxidative stress and systemic inflammation, hallmarks of metabolic impairment. Importantly, these environmental pollutants are also linked to epigenetic modifications, including aberrant DNA methylation, histone modifications, and microRNA dysregulation, which further disrupt insulin sensitivity and β-cell function. This review explores the possible mechanistic crosstalk between AhR/CYP1 pathway activation and epigenetic alterations in the context of diabetes development. By integrating findings from epidemiology, in vivo, and in vitro studies, we provide a summary of how environmental exposures may influence diabetes risk through epigenetic mechanisms. Understanding these interactions not only advances our knowledge of DM etiology but also highlights novel molecular targets for preventive and therapeutic strategies.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous inflammation alters nociceptor electrophysiology in guinea pigs but not rats. 皮肤炎症改变豚鼠的痛觉感受器电生理，而大鼠没有。

Biomolecules & biomedicine Pub Date : 2025-05-19 DOI: 10.17305/bb.2025.12195

Laiche Djouhri, Ahmed Eliwa, Alghalya Al-Emadi, Yehia Y Hussein, Hissa Al-Suwaidi, Al-Jouhara Albaloshi, Ayman Mustafa, Mohammed Seed Ahmed

{"title":"Cutaneous inflammation alters nociceptor electrophysiology in guinea pigs but not rats.","authors":"Laiche Djouhri, Ahmed Eliwa, Alghalya Al-Emadi, Yehia Y Hussein, Hissa Al-Suwaidi, Al-Jouhara Albaloshi, Ayman Mustafa, Mohammed Seed Ahmed","doi":"10.17305/bb.2025.12195","DOIUrl":"https://doi.org/10.17305/bb.2025.12195","url":null,"abstract":"Inflammatory pain hypersensitivity is believed to result, in part, from increased excitability of nociceptive dorsal root ganglion (DRG) neurons. We previously demonstrated in guinea pigs that hindlimb inflammation induces electrophysiological changes in these neurons, including faster action potential (AP) and afterhyperpolarization (AHP) kinetics. Given that rats and guinea pigs are distinct species with notable differences in genetic composition and physiology, we hypothesized that cutaneous inflammation would have different effects on the electrophysiological properties of nociceptive DRG neurons in rats-the predominant rodent model for pain research. To test this hypothesis, we performed intracellular voltage recordings from DRG neurons (n = 430) in deeply anesthetized, untreated (control) and complete Freund's adjuvant (CFA)-treated rats and guinea pigs. C-, Aδ-, and Aβ-nociceptors were identified based on their dorsal root conduction velocities (CVs) and responses to natural noxious stimuli. Consistent with our hypothesis, we observed no significant changes in any electrophysiological variables in rat nociceptive neurons four days after CFA-induced hindlimb inflammation. In contrast, guinea pig nociceptors exhibited a significant increase in CV and significant decreases in both AP and AHP durations. The inflammation-induced shortening of absolute and relative refractory periods likely contributes to increased firing frequency in nociceptive nerve fibers, thereby promoting inflammatory pain hypersensitivity. These findings suggest species-specific differences in peripheral neuronal mechanisms underlying inflammatory pain, potentially due to variation in ion channel expression and/or function in DRG neurons between rats and guinea pigs. Given the genetic and metabolic similarities between guinea pigs and humans, further research is warranted to determine whether guinea pigs may serve as a more accurate model of chronic inflammatory pain than rats.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0