Biomolecules & biomedicine最新文献_第2页

Kidney-directed AAV9 Klotho gene delivery improves renal function in a mouse model of chronic kidney disease. 肾脏定向的AAV9 Klotho基因递送改善慢性肾脏疾病小鼠模型的肾功能。

Biomolecules & biomedicine Pub Date : 2026-10-01 DOI: 10.17305/bb.2026.14798

Chaoying Yan, Li Jian

{"title":"Kidney-directed AAV9 Klotho gene delivery improves renal function in a mouse model of chronic kidney disease.","authors":"Chaoying Yan, Li Jian","doi":"10.17305/bb.2026.14798","DOIUrl":"https://doi.org/10.17305/bb.2026.14798","url":null,"abstract":"Chronic kidney disease (CKD) is associated with progressive renal dysfunction, Klotho deficiency, and activation of profibrotic signaling. This study evaluated the functional and molecular effects of kidney-directed adeno-associated virus serotype 9 (AAV9)-mediated human Klotho delivery in experimental CKD. Forty-eight male mice were studied in six groups: sham, untreated 5/6 nephrectomy, empty vector, low- and high-dose AAV9-Klotho, and recombinant Klotho. Vector was administered via the renal vein at week 4. The primary endpoint was week-16 serum creatinine, analyzed using a baseline-adjusted mixed model for repeated measures. High-dose AAV9-Klotho was associated with lower serum creatinine than empty vector (adjusted difference, -20.6 µmol/L; 95% confidence interval, -33.3 to -7.9; p = 0.0018), with concordant changes in blood urea nitrogen, proteinuria, and urinary albumin-to-creatinine ratio. Human Klotho expression increased with vector dose, accompanied by recovery of endogenous mouse Klotho. Fibrosis-associated and inflammatory markers, transforming growth factor-β receptor I abundance, and phosphorylation of mothers against decapentaplegic homologs 2 and 3 (Smad2/3) were lower after treatment, whereas transforming growth factor-β1 (TGF-β1) abundance remained unchanged. These findings associate kidney-directed AAV9-Klotho delivery with improved renal-function measures and modulation of TGF-β1/Smad2/3 signaling in experimental CKD. Histological antifibrotic efficacy and direct receptor-level mechanisms remain unconfirmed.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conditional cancer-specific survival in breast cancer with de novo bone metastases: A SEER-based study. 伴有新发骨转移的乳腺癌的有条件癌症特异性生存率：一项基于seer的研究。

Biomolecules & biomedicine Pub Date : 2026-09-30 DOI: 10.17305/bb.2026.14614

Shilong Wang, Long Jia

{"title":"Conditional cancer-specific survival in breast cancer with de novo bone metastases: A SEER-based study.","authors":"Shilong Wang, Long Jia","doi":"10.17305/bb.2026.14614","DOIUrl":"https://doi.org/10.17305/bb.2026.14614","url":null,"abstract":"Baseline survival estimates do not capture evolving prognosis in breast cancer with de novo bone metastases. We aimed to develop and internally evaluate a prognostic framework integrating baseline and conditional cancer-specific survival (CSS) estimates. Using the Surveillance, Epidemiology, and End Results (SEER) database, we retrospectively analysed 4,013 women diagnosed in 2010-2015 and randomly allocated 70% to training and 30% to internal validation. Random survival forest-assisted variable selection and Cox proportional hazards regression were used to construct baseline and conditional survival nomograms. Seven predictors were retained: age, primary tumour surgery, nodal stage, molecular subtype, and concomitant brain, liver, and lung metastases. The validation concordance index was 0.662 (95% confidence interval: 0.642-0.681), indicating moderate discrimination. Model-averaged probabilities of surviving to 10 years conditional on 5-year survival were 43.0% and 43.7% in training and validation, respectively, compared with unconditional CSS estimates of 17.2% and 17.7%. Landmark calibration was satisfactory for predicting subsequent 3-year CSS among 3-year survivors; other conditional scenarios were not empirically validated. The framework may support updated prognostic assessment once initial treatment status is known. However, 10-year estimates remain exploratory because of limited late follow-up and non-proportional hazards, and external validation is needed before clinical application.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149082831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer-cell-intrinsic GAS2L3 promotes migration and is associated with poor prognosis in cholangiocarcinoma. 在胆管癌中，癌细胞固有的GAS2L3促进迁移并与不良预后相关。

Biomolecules & biomedicine Pub Date : 2026-09-30 DOI: 10.17305/bb.2026.14818

Natnicha Paungpan, Kulthida Vaeteewoottacharn, Ryusho Kariya, Saowaluk Saisomboon, Sakda Waraasawapati, Han Ni Zin Myint, Kanlayanee Sawanyawisuth, Sukanya Luang, Worachart Lert-Itthiporn, Wunchana Seubwai, Chawalit Pairojkul, Seiji Okada

{"title":"Cancer-cell-intrinsic GAS2L3 promotes migration and is associated with poor prognosis in cholangiocarcinoma.","authors":"Natnicha Paungpan, Kulthida Vaeteewoottacharn, Ryusho Kariya, Saowaluk Saisomboon, Sakda Waraasawapati, Han Ni Zin Myint, Kanlayanee Sawanyawisuth, Sukanya Luang, Worachart Lert-Itthiporn, Wunchana Seubwai, Chawalit Pairojkul, Seiji Okada","doi":"10.17305/bb.2026.14818","DOIUrl":"https://doi.org/10.17305/bb.2026.14818","url":null,"abstract":"The role of growth arrest-specific 2-like 3 (GAS2L3) in cholangiocarcinoma (CCA) cells remains unclear. This study explored GAS2L3 expression, its prognostic value, and its impact on CCA cell migration. We integrated public transcriptomic data with immunohistochemistry from tumor tissues of 55 patients who underwent resection for intrahepatic CCA. Survival associations were determined using Kaplan-Meier analysis and exploratory multivariable Cox regression. To assess proliferation, actin organization, three-dimensional migration, and epithelial-mesenchymal transition (EMT) markers, we employed stable GAS2L3 knockdown in KKU-213A and KKU-055 cells. GAS2L3 transcript levels were significantly higher in tumors compared to non-tumor tissues (p < 0.01), though this transcript-level association with survival was not significant (p = 0.254). However, high GAS2L3 protein expression correlated with shorter overall survival (adjusted hazard ratio, 1.921; 95% confidence interval, 1.082-3.411; p = 0.026). GAS2L3 knockdown suppressed migration in both cell lines without significantly affecting proliferation over 72 hours. In KKU-055 cells, we observed actin redistribution and reduced cell size, while KKU-213A morphology remained largely unchanged. EMT marker responses varied by cell line: Slug and vimentin decreased in KKU-213A, whereas claudin-1 and E-cadherin increased in KKU-055. These findings suggest an intracellular role for GAS2L3 in CCA cell migration and highlight its potential as a prognostic biomarker. Further independent clinical and mechanistic validation is warranted.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathologic complete response and residual disease after neoadjuvant chemotherapy in breast cancer: Comparison of contrast-enhanced mammography, DCE-MRI, and ¹⁸F-FDG PET/CT. 乳腺癌新辅助化疗后的病理完全缓解和残留疾病：对比增强乳房x线摄影、DCE-MRI和¹⁸F-FDG PET/CT的比较。

Biomolecules & biomedicine Pub Date : 2026-09-28 DOI: 10.17305/bb.2026.14598

Ebru Ozan Sanhal, İmren Mutlu, Evrim Sürer Budak, Veli Vural, İsmail Zihni, Kübra Simşek, Sema Sezgin Göksu, Cumhur Arıcı

{"title":"Pathologic complete response and residual disease after neoadjuvant chemotherapy in breast cancer: Comparison of contrast-enhanced mammography, DCE-MRI, and ¹⁸F-FDG PET/CT.","authors":"Ebru Ozan Sanhal, İmren Mutlu, Evrim Sürer Budak, Veli Vural, İsmail Zihni, Kübra Simşek, Sema Sezgin Göksu, Cumhur Arıcı","doi":"10.17305/bb.2026.14598","DOIUrl":"https://doi.org/10.17305/bb.2026.14598","url":null,"abstract":"Accurate assessment of breast cancer response to neoadjuvant chemotherapy (NAC) is important for surgical planning. We compared contrast-enhanced mammography (CEM), dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), and fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography (¹⁸F-FDG PET/CT) for predicting breast pathologic complete response (pCR) and assessing residual disease. This retrospective single-center study included 76 patients who underwent all three modalities before and after NAC, followed by surgery. Postoperative histopathology was the reference standard; breast pCR was defined as the absence of residual invasive carcinoma in the breast, irrespective of residual ductal carcinoma in situ. Breast pCR occurred in 28 patients (36.8%). CEM had higher sensitivity than DCE-MRI (89.3% versus 60.7%; p = 0.012) and the highest negative predictive value (92.1%), whereas DCE-MRI had the highest specificity (85.4%) and positive predictive value (70.8%). Accuracy was 79.0%, 76.3%, and 73.7% for CEM, DCE-MRI, and ¹⁸F-FDG PET/CT, respectively, without a significant difference among modalities (p = 0.661). Corresponding areas under the receiver operating characteristic curve were 0.841, 0.844, and 0.769. Among 48 patients with residual invasive disease, intraclass correlation coefficients for CEM and DCE-MRI measurements versus pathology were 0.733 and 0.773, respectively. However, 95% limits of agreement were wide (-34.16 to 25.16 mm and -29.26 to 20.93 mm, respectively), indicating substantial individual-level discrepancies. CEM showed promise for post-NAC breast response assessment in this selected cohort, but the findings do not establish equivalence or interchangeability with DCE-MRI. Individual tumor size estimates require caution, and prospective multicenter validation is warranted.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjunctive screening and risk stratification performance of neutrophil-to-lymphocyte ratio for gestational diabetes mellitus: A systematic review and meta-analysis. 妊娠期糖尿病中性粒细胞与淋巴细胞比值的辅助筛查和风险分层表现：一项系统回顾和荟萃分析。

Biomolecules & biomedicine Pub Date : 2026-09-28 DOI: 10.17305/bb.2026.14713

Ling Chen, Wei Hu, Fei Zhao, Qian Pan, Chunhong Li, Xiangyuan Ju, Rennan Yao

{"title":"Adjunctive screening and risk stratification performance of neutrophil-to-lymphocyte ratio for gestational diabetes mellitus: A systematic review and meta-analysis.","authors":"Ling Chen, Wei Hu, Fei Zhao, Qian Pan, Chunhong Li, Xiangyuan Ju, Rennan Yao","doi":"10.17305/bb.2026.14713","DOIUrl":"https://doi.org/10.17305/bb.2026.14713","url":null,"abstract":"The neutrophil-to-lymphocyte ratio (NLR) is an inexpensive inflammatory marker with uncertain value for gestational diabetes mellitus (GDM) risk assessment. This systematic review and meta-analysis evaluated NLR for first-trimester risk stratification and second-trimester adjunctive screening of GDM. PubMed, Embase, Web of Science, and the Cochrane Library were searched from inception to April 15, 2026. Risk of bias was assessed using the Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) tool. Sensitivity and specificity were pooled separately by trimester, and summary receiver operating characteristic curves were fitted using the Moses-Littenberg method. Eleven studies comprising 12 independent datasets used the oral glucose tolerance test (OGTT) as the reference standard. Pooled sensitivity, specificity, and area under the curve (AUC), with 95% confidence intervals (CIs), were 0.702 (0.660-0.742), 0.426 (0.401-0.452), and 0.694 (0.559-0.828), respectively, in the first trimester and 0.665 (0.632-0.697), 0.644 (0.618-0.670), and 0.798 (0.723-0.873) in the second trimester. At cutoffs ≤ 3.0, exploratory AUC estimates were 0.845 and 0.850, respectively, but subgroup differences were not formally tested. All studies were at high risk of index-test bias from post hoc threshold selection, seven used case-control designs, and heterogeneity was substantial. NLR may have an adjunctive role in GDM risk assessment but should not replace OGTT or serve as a standalone screening test. No clinical cutoff can be recommended; prospective studies with prespecified thresholds and independent validation cohorts are required.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concomitant chemoradiotherapy for head and neck squamous cell carcinoma: Real-world treatment delivery, toxicity, and survival in Bosnia and Herzegovina. 头颈部鳞状细胞癌的同步放化疗：波斯尼亚和黑塞哥维那真实世界的治疗递送、毒性和生存率。

Biomolecules & biomedicine Pub Date : 2026-09-23 DOI: 10.17305/bb.2026.14774

Gordana Berić Jozić, Mirela Mabić, Monika Glibić, Sanda Čale, Krešimir Tomić, Inga Marijanović

{"title":"Concomitant chemoradiotherapy for head and neck squamous cell carcinoma: Real-world treatment delivery, toxicity, and survival in Bosnia and Herzegovina.","authors":"Gordana Berić Jozić, Mirela Mabić, Monika Glibić, Sanda Čale, Krešimir Tomić, Inga Marijanović","doi":"10.17305/bb.2026.14774","DOIUrl":"https://doi.org/10.17305/bb.2026.14774","url":null,"abstract":"Real-world evidence on concomitant chemoradiotherapy (CCRT) for head and neck squamous cell carcinoma (HNSCC) in Bosnia and Herzegovina remains limited. We aimed to characterize treatment delivery, toxicity, and survival and identify modifiable barriers to optimal care. This retrospective single-institution cohort study included 116 consecutive patients with HNSCC receiving curative-intent definitive or adjuvant CCRT at University Hospital Mostar during 2012-2022, with follow-up through May 2025. Overall survival (OS) from diagnosis and event-free survival (EFS) from radiotherapy initiation were evaluated using Kaplan-Meier estimates and univariable Cox regression. Most patients (88.8%) presented with stage IVA-IVB disease. Radiotherapy interruptions of ≥4 days occurred in 50.9%, and 51.7% discontinued concomitant chemotherapy. Among 107 cisplatin-exposed patients, 29.0% received a cumulative dose ≥200 mg/m². Median follow-up was 85 months for OS and 78 months for EFS. Estimated 2- and 5-year OS rates were 50.9% and 32.9%, respectively; corresponding EFS rates were 45.7% and 31.0%. Both outcomes differed across stage groups (p < 0.001 for both), with exploratory univariable analyses indicating poorer outcomes in stage IVB than stage III. Acute grade ≥3 toxicity occurred in 74.1%, including one treatment-related death; late toxicity of any grade was documented in 47.4%. This cohort highlights substantial toxicity and frequent treatment-delivery modifications in routine practice. The findings identify priorities for improving treatment continuity, cisplatin delivery, proactive supportive care, renal monitoring, and multidisciplinary coordination, while survival associations remain exploratory.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149027035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Notch signaling and ferroptosis - Context-dependent regulation and therapeutic implications: A review. Notch信号和铁下垂-环境依赖性调节和治疗意义：综述。

Biomolecules & biomedicine Pub Date : 2026-09-22 DOI: 10.17305/bb.2026.14693

Zhongyue Wu, Mingren Liu, Bowei Liu, Jun Huang, Zhiwei Xia

{"title":"Notch signaling and ferroptosis - Context-dependent regulation and therapeutic implications: A review.","authors":"Zhongyue Wu, Mingren Liu, Bowei Liu, Jun Huang, Zhiwei Xia","doi":"10.17305/bb.2026.14693","DOIUrl":"https://doi.org/10.17305/bb.2026.14693","url":null,"abstract":"Ferroptosis is an iron-dependent form of regulated cell death driven by lipid peroxidation, but its regulation by Notch signaling remains incompletely understood. This narrative review examines the mechanisms and disease relevance of Notch-ferroptosis interactions. Evidence from experimental models and patient-tissue analyses is synthesized into three categories: Notch-mediated regulation, indirect or Notch-independent associations, and Notch-induced ferroptosis sensitivity. In selected models, Notch activation suppresses ferroptosis by supporting nuclear factor E2-related factor 2 (NRF2)-dependent antioxidant defenses, maintaining selenium uptake, or stabilizing glutathione peroxidase 4 (GPX4). Conversely, loss of protective Notch signaling is associated with increased ferroptosis in hepatic stellate cells. Context-specific Notch-dependent promotion has been reported in hepatocellular carcinoma, although receptor-proximal mechanisms remain unresolved. In glioblastoma, Notch activation creates a metabolically vulnerable state that increases ferroptosis susceptibility without directly initiating ferroptosis. Other reported relationships involve Notch-independent mechanisms or remain associative. Cell type, metabolic state, and microenvironment contribute to these divergent outcomes. Overall, the evidence is predominantly preclinical, with direct causal support restricted to selected models and limited clinical validation. Therapeutic strategies targeting Notch-ferroptosis interactions should therefore be tailored to the underlying mechanism and disease context, with further mechanistic confirmation and clinical validation required before therapeutic translation.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149018795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dexmedetomidine versus midazolam during total thyroidectomy with intraoperative neuromonitoring: A prospective non-randomized study. 右美托咪定与咪达唑仑在全甲状腺切除术中术中神经监测：一项前瞻性非随机研究。

Biomolecules & biomedicine Pub Date : 2026-09-18 DOI: 10.17305/bb.2026.14894

Suat Evirgen, Harun Tolga Duran, Bülent Meriç Çam, Mürsel Kahveci, Osman Özgür Kılınç, Şirin Çetin

{"title":"Dexmedetomidine versus midazolam during total thyroidectomy with intraoperative neuromonitoring: A prospective non-randomized study.","authors":"Suat Evirgen, Harun Tolga Duran, Bülent Meriç Çam, Mürsel Kahveci, Osman Özgür Kılınç, Şirin Çetin","doi":"10.17305/bb.2026.14894","DOIUrl":"10.17305/bb.2026.14894","url":null,"abstract":"Anesthetic adjuncts may influence intraoperative neuromonitoring (IONM) during thyroidectomy. We compared dexmedetomidine and midazolam as adjuncts to propofol-based total intravenous anesthesia (TIVA). This prospective, single-center, non-randomized, unblinded study included 60 adults undergoing elective total thyroidectomy with intermittent IONM, assigned alternately to dexmedetomidine or midazolam (30 per group). The primary outcome was recurrent laryngeal nerve (RLN) identification time from skin incision on the first dissected side; secondary outcomes were exploratory. Median (interquartile range) RLN identification time was shorter with dexmedetomidine (21.0 [19.0-26.8] versus 27.0 [21.2-31.0] minutes; Hodges-Lehmann median difference, -4.0 minutes; 95% confidence interval [CI], -7.0 to -1.0; p < 0.001). Median vagus nerve identification time was also shorter (12.5 versus 17.0 minutes; p < 0.001), whereas total operative time did not differ. Pre-resection electromyography (EMG) amplitudes were lower with dexmedetomidine at the vagus nerve (810 versus 905 µV) and RLN (835 versus 1000 µV; both p < 0.001), but all recorded amplitudes were ≥700 µV, with no loss of signal. Median extubation and recovery times were shorter (8.0 versus 13.0 and 6.0 versus 9.0 minutes, respectively; both p < 0.001). Bradycardia occurred in 16.7% versus 0% of patients (p = 0.052). Dexmedetomidine was associated with shorter nerve identification and early recovery times, although lower EMG amplitudes and observed bradycardia warrant caution. These findings require randomized confirmation; postoperative vocal cord function was not assessed, precluding conclusions about nerve safety.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":"2433-2442"},"PeriodicalIF":0.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13667946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149009455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum soluble endoglin is associated with clinical and endoscopic disease activity in ulcerative colitis. 血清可溶性内啡肽与溃疡性结肠炎的临床和内镜疾病活动有关。

Biomolecules & biomedicine Pub Date : 2026-09-17 DOI: 10.17305/bb.2026.14722

Tingting Wang, Ying Jiang, Chen Chen, Zhou Zhou, Caiping Gao, Jinxia Wang, Yan Pan

{"title":"Serum soluble endoglin is associated with clinical and endoscopic disease activity in ulcerative colitis.","authors":"Tingting Wang, Ying Jiang, Chen Chen, Zhou Zhou, Caiping Gao, Jinxia Wang, Yan Pan","doi":"10.17305/bb.2026.14722","DOIUrl":"https://doi.org/10.17305/bb.2026.14722","url":null,"abstract":"Endothelial dysfunction is implicated in ulcerative colitis (UC), but the value of soluble endoglin (sENG) for assessing disease activity remains unclear. We investigated associations between serum sENG and clinical and endoscopic activity in UC. This prospective, single-center case-control study enrolled 105 patients with UC and 68 healthy controls. Serum sENG was measured by enzyme-linked immunosorbent assay; clinical and endoscopic activity were defined by partial Mayo scores ≥3 and Mayo endoscopic subscores ≥2, respectively. Receiver operating characteristic analysis and multivariable logistic regression evaluated discriminatory performance and adjusted associations. Serum sENG was higher in patients with UC than in controls (median, 6.94 vs. 3.40 ng/mL; p < 0.001) and correlated with clinical and endoscopic scores (Spearman's correlation coefficients, 0.596 and 0.625, respectively; both p < 0.0001). Areas under the curve were 0.786 for distinguishing UC from controls, 0.765 for clinical activity, and 0.808 for endoscopic activity. Higher sENG remained independently associated with clinically active disease (adjusted odds ratio, 1.324; 95% confidence interval, 1.132-1.600; p = 0.001). Positive correlations were also observed with interleukin-6, tumor necrosis factor-α, interferon-γ, and interleukin-17A (all p < 0.001). Serum sENG shows potential as a noninvasive biomarker of UC activity, but its clinical utility requires external validation and comparison with established biomarkers.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148992716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LNK expression, subcellular localisation and survival associations in breast, cervical and colorectal cancer. LNK在乳腺癌、宫颈癌和结直肠癌中的表达、亚细胞定位和生存相关性

Biomolecules & biomedicine Pub Date : 2026-09-17 DOI: 10.17305/bb.2026.14287

Jessica C Mantilla-Ollarves, Alejandro Sánchez-Carballido, Alejandro Ordaz-Ramos, Cecilia Aguilar, Miguel Tapia-Rodríguez, Liliana Cortés-Ballinas, Laura Velázquez, Leticia Rocha-Zavaleta

{"title":"LNK expression, subcellular localisation and survival associations in breast, cervical and colorectal cancer.","authors":"Jessica C Mantilla-Ollarves, Alejandro Sánchez-Carballido, Alejandro Ordaz-Ramos, Cecilia Aguilar, Miguel Tapia-Rodríguez, Liliana Cortés-Ballinas, Laura Velázquez, Leticia Rocha-Zavaleta","doi":"10.17305/bb.2026.14287","DOIUrl":"https://doi.org/10.17305/bb.2026.14287","url":null,"abstract":"The role of lymphocyte adaptor protein (LNK) in breast, cervical and colorectal cancer remains incompletely characterised. We examined LNK expression, subcellular localisation and associations with survival across these cancers. Gene Expression Profiling Interactive Analysis and cBioPortal were used to assess LNK messenger ribonucleic acid (mRNA) expression, while Kaplan-Meier Plotter was used for exploratory survival analyses. Western blotting and quantitative confocal immunofluorescence assessed protein expression and localisation in 12 cancer cell lines and three non-tumoural controls. LNK mRNA expression varied among tumour subtypes and was significantly lower in cervical tumours than in adjacent normal tissue. Higher expression was associated with longer overall and recurrence-free survival in triple-negative breast cancer but shorter survival for both endpoints in colorectal cancer; no significant associations were observed in cervical cancer. LNK was detected in the nucleus and cytoplasm of all cancer cell lines. Significant nuclear predominance was observed in one of four triple-negative breast cancer cell lines and three of four cervical cancer cell lines, whereas their non-tumoural controls showed cytoplasmic predominance. Colorectal cancer cell lines displayed heterogeneous subcellular distributions. These exploratory findings suggest cancer type-specific associations between LNK expression and survival that require independent validation, while nuclear localisation warrants investigation of potential nuclear functions.","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148992745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0